RchiOBHm_Chr6g0250101

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000184
Physical Location & Seq
Reverse (-)
5773419 .. 5774024
606 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22420

Sequence Viewer

Length: 606 bp
ATGGACAGTCCGCCTCTTCGTCTCCGCCGCTCAAACCTCACCATCAACCGTCGCCTCTTTAACTTGTTTGACTACAAACCATCTCCCTTAACAAAACCTCACAAAAACCTCAAGAGCTCCGACGTCGTCGTCGTCGCTGACCCTTCCCGCAACCTCTGGTTCCGCCTCTACACTCCCACCGCCGCTACCACCAAGCTCCCCATCATCGTCTTCTTCCACGGTGGCGGGTTCGAGGTCATGTCTGCCGCTTCAAAACCCTACGACGACTTCTGTCAACGCCTCGCTGGCGAGATCCCCGCCGTCGTCGTCTCCGTCAACTACCGCCTCGAGCCGGAGTATCGGTATCCGTGCCAGTACGACGACTGTTTCGACGTCCTCAAGTTCATCGACGACAGCAGCTTGTTTGAGGGTGCGAATCTCGAGCAGTGTTTTCTAGCCTGGAACATAGCGTACCACGTGGCGATCAGAGCGAGCGGACACGAGTTTCGGGATCTGAAGGTGGTTGGGATCTTGGCGATTCAGCCGTTTTTCGGCGGAGAAGTGCGGACAGAATCGGAGAAGAGAATGAAGAGTATGCCGCTGGTGAACGTAAAGCGGACGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

201

Amino Acids

23.0

Weight (kDa)

8.64

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 55 - 109 3.6e-08 Carboxylesterase family
BD-FAE PF20434 56 - 131 3.2e-09 BD-FAE
Abhydrolase_3 PF07859 69 - 191 7.9e-24 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 2 cut(s) 126, 375
AccBSI CCGCTC 2 cut(s) 30, 474
AclWI GGATC 3 cut(s) 286, 498, 515
AcuI CTGAAG 1 cut(s) 515
AcvI CACGTG 1 cut(s) 457
AcyI GRCGYC 2 cut(s) 123, 372
AfaI GTAC 2 cut(s) 356, 452
AfiI CCNNNNNNNGG 2 cut(s) 331, 530
AgsI TTSAA 1 cut(s) 252
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 3 cut(s) 117, 196, 399
AluI AGCT 3 cut(s) 117, 196, 399
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 2 cut(s) 26, 313
AlwI GGATC 3 cut(s) 286, 498, 515
Ama87I CYCGRG 2 cut(s) 326, 419
ApeKI GCWGC 1 cut(s) 396
ArsI GACNNNNNNTTYG 4 cut(s) 350, 382, 468, 500
AsuHPI GGTGA 2 cut(s) 31, 595
AvaI CYCGRG 2 cut(s) 326, 419
BanII GRGCYC 1 cut(s) 119
BauI CACGAG 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 408
BccI CCATC 3 cut(s) 50, 88, 209
BceAI ACGGC 2 cut(s) 284, 508
BciT130I CCWGG 1 cut(s) 439
BciVI GTATCC 1 cut(s) 354
BcoDI GTCTC 2 cut(s) 26, 313
BfaI CTAG 2 cut(s) 434, 604
BfuI GTATCC 1 cut(s) 354
BglI GCCNNNNNGGC 1 cut(s) 285
BisI GCNGC 5 cut(s) 28, 183, 246, 397, 578
BlsI GCNGC 5 cut(s) 29, 184, 247, 398, 579
Bme1390I CCNGG 1 cut(s) 439
BmeT110I CYCGRG 2 cut(s) 326, 419
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 1 cut(s) 439
BoxI GACNNNNGTC 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 202
BpuEI CTTGAG 2 cut(s) 95, 362
BsaAI YACGTR 1 cut(s) 457
BsaHI GRCGYC 2 cut(s) 123, 372
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 530
Bse1I ACTGG 1 cut(s) 352
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 331, 530
BseNI ACTGG 1 cut(s) 352
BseXI GCAGC 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 119
BsiHKCI CYCGRG 2 cut(s) 326, 419
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 331, 530
BsmAI GTCTC 2 cut(s) 26, 313
BsmBI CGTCTC 2 cut(s) 26, 313
BsoBI CYCGRG 2 cut(s) 326, 419
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 4 cut(s) 291, 462, 490, 507
BspLI GGNNCC 1 cut(s) 161
BspPI GGATC 3 cut(s) 286, 498, 515
BsrBI CCGCTC 2 cut(s) 30, 474
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 4 cut(s) 291, 462, 490, 507
BssNI GRCGYC 2 cut(s) 123, 372
BssSI CACGAG 1 cut(s) 479
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 4 cut(s) 8, 50, 221, 365
Bst6I CTCTTC 3 cut(s) 21, 554, 563
BstACI GRCGYC 2 cut(s) 123, 372
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 286, 472
BstDSI CCRYGG 1 cut(s) 217
BstKTI GATC 4 cut(s) 294, 465, 493, 510
BstMAI GTCTC 2 cut(s) 26, 313
BstMBI GATC 4 cut(s) 291, 462, 490, 507
BstMWI GCNNNNNNNGC 2 cut(s) 285, 467
BstNI CCWGG 1 cut(s) 439
BstPAI GACNNNNGTC 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 437
BstV1I GCAGC 1 cut(s) 408
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 3 cut(s) 291, 490, 507
BstYI RGATCY 3 cut(s) 291, 490, 507
BsuI GTATCC 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 217
BtsI GCAGTG 1 cut(s) 431
BtsIMutI CAGTG 1 cut(s) 431
Cac8I GCNNGC 2 cut(s) 286, 472
Csp6I GTAC 2 cut(s) 355, 451
CviAII CATG 1 cut(s) 238
CviJI RGCY 6 cut(s) 117, 196, 331, 399, 437, 523
CviKI_1 RGCY 6 cut(s) 117, 196, 331, 399, 437, 523
CviQI GTAC 2 cut(s) 355, 451
DpnI GATC 4 cut(s) 293, 464, 492, 509
DpnII GATC 4 cut(s) 291, 462, 490, 507
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
Eam1104I CTCTTC 3 cut(s) 21, 554, 563
EarI CTCTTC 3 cut(s) 21, 554, 563
EciI GGCGGA 3 cut(s) 14, 152, 549
Ecl136II GAGCTC 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 117
Eco57I CTGAAG 1 cut(s) 515
Eco72I CACGTG 1 cut(s) 457
Eco88I CYCGRG 2 cut(s) 326, 419
EcoICRI GAGCTC 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 119
Esp3I CGTCTC 2 cut(s) 26, 313
FaeI CATG 1 cut(s) 241
FaiI YATR 3 cut(s) 239, 446, 575
FatI CATG 1 cut(s) 237
FauI CCCGC 3 cut(s) 155, 218, 304
Fnu4HI GCNGC 5 cut(s) 28, 183, 246, 397, 578
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 5 cut(s) 28, 183, 246, 397, 578
FspBI CTAG 2 cut(s) 434, 604
GluI GCNGC 5 cut(s) 28, 183, 246, 397, 578
HapII CCGG 1 cut(s) 332
Hin1I GRCGYC 2 cut(s) 123, 372
Hin1II CATG 1 cut(s) 241
HincII GTYRAC 2 cut(s) 275, 316
HindII GTYRAC 2 cut(s) 275, 316
HinfI GANTC 3 cut(s) 415, 517, 551
HpaII CCGG 1 cut(s) 332
HphI GGTGA 2 cut(s) 31, 595
Hpy166II GTNNAC 3 cut(s) 275, 316, 586
Hpy188I TCNGA 4 cut(s) 121, 467, 495, 556
Hpy188III TCNNGA 3 cut(s) 112, 419, 488
Hpy8I GTNNAC 3 cut(s) 275, 316, 586
HpyAV CCTTC 2 cut(s) 153, 490
HpyCH4III ACNGT 4 cut(s) 8, 50, 221, 365
HpyCH4IV ACGT 4 cut(s) 123, 372, 456, 588
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 467
HpySE526I ACGT 4 cut(s) 123, 372, 456, 588
Hsp92I GRCGYC 2 cut(s) 123, 372
Hsp92II CATG 1 cut(s) 241
Kzo9I GATC 4 cut(s) 291, 462, 490, 507
LmnI GCTCC 2 cut(s) 122, 201
LpnPI CCDG 7 cut(s) 142, 270, 345, 365, 424, 451, 566
Lsp1109I GCAGC 1 cut(s) 408
MaeI CTAG 2 cut(s) 434, 604
MaeII ACGT 4 cut(s) 123, 372, 456, 588
MalI GATC 4 cut(s) 293, 464, 492, 509
MbiI CCGCTC 2 cut(s) 30, 474
MboI GATC 4 cut(s) 291, 462, 490, 507
MboII GAAGA 5 cut(s) 8, 202, 205, 571, 580
MflI RGATCY 3 cut(s) 291, 490, 507
MhlI GDGCHC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 144
MseI TTAA 2 cut(s) 60, 89
MspA1I CMGCKG 1 cut(s) 580
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 439
MvaI CCWGG 1 cut(s) 439
MwoI GCNNNNNNNGC 2 cut(s) 285, 467
NdeII GATC 4 cut(s) 291, 462, 490, 507
NlaIII CATG 1 cut(s) 241
NlaIV GGNNCC 1 cut(s) 161
PaeR7I CTCGAG 2 cut(s) 326, 419
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 309, 366
PfeI GAWTC 3 cut(s) 415, 517, 551
PflFI GACNNNGTC 1 cut(s) 125
PkrI GCNGC 5 cut(s) 29, 184, 247, 398, 579
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 457
PshAI GACNNNNGTC 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 437
PspCI CACGTG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 1 cut(s) 161
PspXI VCTCGAGB 1 cut(s) 326
PsrI GAACNNNNNNTAC 2 cut(s) 434, 466
PsuI RGATCY 3 cut(s) 291, 490, 507
PsyI GACNNNGTC 1 cut(s) 125
RsaI GTAC 2 cut(s) 356, 452
RsaNI GTAC 2 cut(s) 355, 451
SacI GAGCTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 60, 89
SatI GCNGC 5 cut(s) 28, 183, 246, 397, 578
Sau3AI GATC 4 cut(s) 291, 462, 490, 507
ScrFI CCNGG 1 cut(s) 439
SduI GDGCHC 1 cut(s) 119
Sfr274I CTCGAG 2 cut(s) 326, 419
SlaI CTCGAG 2 cut(s) 326, 419
SmlI CTYRAG 4 cut(s) 110, 326, 377, 419
SmoI CTYRAG 4 cut(s) 110, 326, 377, 419
SspMI CTAG 2 cut(s) 434, 604
SstI GAGCTC 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 437
TaaI ACNGT 4 cut(s) 8, 50, 221, 365
TaiI ACGT 4 cut(s) 126, 375, 459, 591
TaqI TCGA 5 cut(s) 231, 327, 369, 387, 420
TauI GCSGC 4 cut(s) 30, 185, 248, 580
TfiI GAWTC 3 cut(s) 415, 517, 551
Tru1I TTAA 2 cut(s) 60, 89
Tru9I TTAA 2 cut(s) 60, 89
TscAI CASTG 1 cut(s) 431
TseI GCWGC 1 cut(s) 396
TspDTI ATGAA 2 cut(s) 373, 581
TspGWI ACGGA 2 cut(s) 301, 336
TspRI CASTG 1 cut(s) 431
Tth111I GACNNNGTC 1 cut(s) 125
XhoI CTCGAG 2 cut(s) 326, 419
XspI CTAG 2 cut(s) 434, 604
ZraI GACGTC 2 cut(s) 124, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.