pycom03g13020

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000184
Physical Location & Seq
Reverse (-)
13925538 .. 13926572
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13020.1

Sequence Viewer

Length: 1035 bp
ATGCATGCATACACCGACAGAAAGAGCAAGATGGCCCAAAACAACCCCATATCGCCGAACCTTCCATGCAAGGTCCGTCTCTTAATCTCTATCCTGACCACCGTCATCGACGCCACTCGCCGCTCCAATGGCACTGTTAACCGCCGTCTTATGAGCCTATTCGACTTCAAAGCCTCCCCTTCGGACAAACCCAACAACGCGGTCAAAACCTCCGACGTAATGGTGGACCCTTCCCGCAACCTCTGGTTCCGCCTCTACGTCCCCACCACCACCGACCCTGCCTCCAAGCTTCCACTTGTTATCTACTTCCACGGTGGTGGATTCGTGTTCTTTTCTGCCAACTCAAAACCCTACAACGACCTCTGCAAACGTCTCGCCACCAAACTCCCTGCCGTCATCATCTCCGTCAACTACCGCCATGCGCCGGAGCATCGGTATCCTTCCCAATACGAAGACGGCATGGACGTCCTCAAATTCATTGACACAACAAGAATTGAGGGTCTTGATCTCAACAACGTTGACATCACCCGGTGTTTCCTCGCCGGAGACAGCGCCGGCGGCAACCTGGCCCACCACGTGGTAATCAAGACGAGCGACCATGAGTTTTGCCAGATGAGGGTGATGGGACTAATAGCGATACAGCCATTTTTTGGCGGGAAAGAAAGGACCGAATCGGAAACAAGGCTTGCAAAAGAGGGCTTGGCGATCACTTTGGAGCAGACTGACTGGTATTGGAAGGCGTTTTTGCCGGAGGGATCGAATAGGGACCACGCGGTGGTGAACGTGTTTGGGCCAGAGTCAAGCGGTATCTTGGGGGTCAAGTTCCCGGCCACGATTGTTTTCATGGGAGGTTTCGATCCGTTACAAGACTGGCAAAAGATGTACTACCAGGGATTGAAGAAAAAGGGGATACAAGCTTACTTGATCGAGTACCCAAATTCGTTTCATGGATTTTATGTTTTTCCTGAGCTAAAGGAGTCTTCCTTTTTTATTAAGGAGGTGAAGGATTTCATCGACAAGCTATCAACTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

345

Amino Acids

38.93

Weight (kDa)

8.79

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Say1_Mug180 PF10340 77 - 251 1.9e-08 Steryl acetyl hydrolase
COesterase PF00135 81 - 140 3.5e-06 Carboxylesterase family
BD-FAE PF20434 85 - 202 1.7e-12 BD-FAE
Abhydrolase_3 PF07859 100 - 319 4.9e-57 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 468
AccB7I CCANNNNNTGG 3 cut(s) 577, 650, 775
AccBSI CCGCTC 1 cut(s) 123
AccII CGCG 2 cut(s) 200, 773
AclI AACGTT 1 cut(s) 516
AclWI GGATC 2 cut(s) 763, 851
AcoI YGGCCR 1 cut(s) 828
AcsI RAATTY 2 cut(s) 473, 937
AcvI CACGTG 1 cut(s) 577
AcyI GRCGYC 2 cut(s) 111, 465
AdeI CACNNNGTG 3 cut(s) 531, 577, 775
AfaI GTAC 2 cut(s) 884, 932
AfiI CCNNNNNNNGG 5 cut(s) 424, 577, 616, 650, 775
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 2 cut(s) 169, 898
AjnI CCWGG 2 cut(s) 564, 888
AleI CACNNNNGTG 1 cut(s) 315
AluBI AGCT 4 cut(s) 289, 917, 970, 1021
AluI AGCT 4 cut(s) 289, 917, 970, 1021
Alw26I GTCTC 3 cut(s) 83, 377, 540
AlwI GGATC 2 cut(s) 763, 851
AoxI GGCC 4 cut(s) 33, 567, 791, 828
ApoI RAATTY 2 cut(s) 473, 937
Asp700I GAANNNNTTC 1 cut(s) 1007
AspLEI GCGC 2 cut(s) 424, 554
AspS9I GGNCC 7 cut(s) 34, 73, 226, 568, 666, 766, 791
AsuC2I CCSGG 2 cut(s) 529, 827
AsuHPI GGTGA 4 cut(s) 517, 631, 790, 1012
AvaII GGWCC 4 cut(s) 73, 226, 666, 766
BbrPI CACGTG 1 cut(s) 577
BbsI GAAGAC 2 cut(s) 459, 972
BccI CCATC 2 cut(s) 25, 616
BceAI ACGGC 3 cut(s) 129, 377, 472
BcgI CGANNNNNNTGC 2 cut(s) 355, 389
BciT130I CCWGG 2 cut(s) 566, 890
BciVI GTATCC 2 cut(s) 447, 903
BcnI CCSGG 2 cut(s) 529, 827
BcoDI GTCTC 3 cut(s) 83, 377, 540
BfoI RGCGCY 1 cut(s) 555
BfuI GTATCC 2 cut(s) 447, 903
BisI GCNGC 2 cut(s) 121, 559
BlsI GCNGC 2 cut(s) 122, 560
Bme1390I CCNGG 4 cut(s) 529, 566, 827, 890
Bme18I GGWCC 4 cut(s) 73, 226, 666, 766
BmgT120I GGNCC 7 cut(s) 34, 73, 226, 568, 666, 766, 791
BmiI GGNNCC 3 cut(s) 228, 248, 767
BmrFI CCNGG 4 cut(s) 529, 566, 827, 890
BmsI GCATC 1 cut(s) 439
BoxI GACNNNNGTC 1 cut(s) 101
BpiI GAAGAC 2 cut(s) 459, 972
Bpu10I CCTNAGC 1 cut(s) 966
BpuMI CCSGG 2 cut(s) 529, 827
BsaAI YACGTR 1 cut(s) 577
BsaHI GRCGYC 2 cut(s) 111, 465
BsaJI CCNNGG 2 cut(s) 310, 889
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 419, 449
Bsc4I CCNNNNNNNGG 5 cut(s) 424, 577, 616, 650, 775
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 2 cut(s) 731, 875
BseBI CCWGG 2 cut(s) 566, 890
BseDI CCNNGG 2 cut(s) 310, 889
BseLI CCNNNNNNNGG 5 cut(s) 424, 577, 616, 650, 775
BseMII CTCAG 1 cut(s) 957
BseNI ACTGG 2 cut(s) 731, 875
Bsh1236I CGCG 2 cut(s) 200, 773
BshFI GGCC 4 cut(s) 35, 569, 793, 830
BsiSI CCGG 6 cut(s) 425, 529, 543, 555, 749, 827
BslFI GGGAC 3 cut(s) 245, 639, 779
BslI CCNNNNNNNGG 5 cut(s) 424, 577, 616, 650, 775
BsmAI GTCTC 3 cut(s) 83, 377, 540
BsmBI CGTCTC 2 cut(s) 83, 377
BsmFI GGGAC 3 cut(s) 245, 639, 779
BsnI GGCC 4 cut(s) 35, 569, 793, 830
Bsp143I GATC 5 cut(s) 505, 705, 755, 856, 924
BspANI GGCC 4 cut(s) 35, 569, 793, 830
BspCNI CTCAG 1 cut(s) 958
BspFNI CGCG 2 cut(s) 200, 773
BspLI GGNNCC 3 cut(s) 228, 248, 767
BspPI GGATC 2 cut(s) 763, 851
BsrBI CCGCTC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 2 cut(s) 731, 875
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 2 cut(s) 310, 889
BssMI GATC 5 cut(s) 505, 705, 755, 856, 924
BssNI GRCGYC 2 cut(s) 111, 465
Bst2UI CCWGG 2 cut(s) 566, 890
Bst4CI ACNGT 3 cut(s) 103, 136, 314
BstACI GRCGYC 2 cut(s) 111, 465
BstBAI YACGTR 1 cut(s) 577
BstC8I GCNNGC 3 cut(s) 6, 556, 687
BstDEI CTNAG 1 cut(s) 966
BstDSI CCRYGG 1 cut(s) 310
BstFNI CGCG 2 cut(s) 200, 773
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 2 cut(s) 424, 554
BstKTI GATC 5 cut(s) 508, 708, 758, 859, 927
BstMAI GTCTC 3 cut(s) 83, 377, 540
BstMBI GATC 5 cut(s) 505, 705, 755, 856, 924
BstMWI GCNNNNNNNGC 2 cut(s) 129, 558
BstNI CCWGG 2 cut(s) 566, 890
BstNSI RCATGY 1 cut(s) 8
BstPAI GACNNNNGTC 1 cut(s) 101
BstSCI CCNGG 4 cut(s) 527, 564, 825, 888
BstUI CGCG 2 cut(s) 200, 773
BstV2I GAAGAC 2 cut(s) 459, 972
BstXI CCANNNNNNTGG 1 cut(s) 317
BsuI GTATCC 2 cut(s) 447, 903
BsuRI GGCC 4 cut(s) 35, 569, 793, 830
BtgI CCRYGG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 3 cut(s) 6, 556, 687
CfoI GCGC 2 cut(s) 424, 554
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 7 cut(s) 34, 73, 226, 568, 666, 766, 791
CseI GACGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 883, 931
CviAII CATG 7 cut(s) 5, 66, 419, 460, 599, 844, 947
CviQI GTAC 2 cut(s) 883, 931
DdeI CTNAG 1 cut(s) 966
DpnI GATC 5 cut(s) 507, 707, 757, 858, 926
DpnII GATC 5 cut(s) 505, 705, 755, 856, 924
DraIII CACNNNGTG 3 cut(s) 531, 577, 775
EaeI YGGCCR 1 cut(s) 828
EciI GGCGGA 1 cut(s) 239
Eco47I GGWCC 4 cut(s) 73, 226, 666, 766
Eco72I CACGTG 1 cut(s) 577
EcoRII CCWGG 2 cut(s) 564, 888
EcoT22I ATGCAT 2 cut(s) 6, 10
Esp3I CGTCTC 2 cut(s) 83, 377
FaeI CATG 7 cut(s) 8, 69, 422, 463, 602, 847, 950
FaqI GGGAC 3 cut(s) 245, 639, 779
FatI CATG 7 cut(s) 4, 65, 418, 459, 598, 843, 946
FauI CCCGC 2 cut(s) 242, 647
Fnu4HI GCNGC 2 cut(s) 121, 559
Fsp4HI GCNGC 2 cut(s) 121, 559
GlaI GCGC 2 cut(s) 423, 553
GluI GCNGC 2 cut(s) 121, 559
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 4 cut(s) 35, 569, 793, 830
HapII CCGG 6 cut(s) 425, 529, 543, 555, 749, 827
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 424, 554
Hin1I GRCGYC 2 cut(s) 111, 465
Hin1II CATG 7 cut(s) 8, 69, 422, 463, 602, 847, 950
Hin6I GCGC 2 cut(s) 422, 552
HinP1I GCGC 2 cut(s) 422, 552
HincII GTYRAC 3 cut(s) 139, 409, 520
HindII GTYRAC 3 cut(s) 139, 409, 520
HindIII AAGCTT 2 cut(s) 287, 915
HinfI GANTC 4 cut(s) 321, 671, 797, 977
HpaI GTTAAC 1 cut(s) 139
HpaII CCGG 6 cut(s) 425, 529, 543, 555, 749, 827
HphI GGTGA 4 cut(s) 517, 631, 790, 1012
Hpy166II GTNNAC 5 cut(s) 139, 226, 409, 520, 781
Hpy188I TCNGA 3 cut(s) 184, 214, 676
Hpy188III TCNNGA 4 cut(s) 94, 503, 586, 965
Hpy8I GTNNAC 5 cut(s) 139, 226, 409, 520, 781
Hpy99I CGWCG 2 cut(s) 113, 218
HpyAV CCTTC 6 cut(s) 71, 189, 240, 450, 730, 997
HpyCH4III ACNGT 3 cut(s) 103, 136, 314
HpyCH4IV ACGT 7 cut(s) 216, 258, 370, 465, 516, 576, 783
HpyCH4V TGCA 5 cut(s) 4, 8, 69, 366, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 558
HpyF3I CTNAG 1 cut(s) 966
HpySE526I ACGT 7 cut(s) 216, 258, 370, 465, 516, 576, 783
Hsp92I GRCGYC 2 cut(s) 111, 465
Hsp92II CATG 7 cut(s) 8, 69, 422, 463, 602, 847, 950
HspAI GCGC 2 cut(s) 422, 552
KroI GCCGGC 1 cut(s) 554
KroNI GCCGGC 1 cut(s) 556
KspAI GTTAAC 1 cut(s) 139
Kzo9I GATC 5 cut(s) 505, 705, 755, 856, 924
LmnI GCTCC 3 cut(s) 128, 427, 715
LweI GCATC 1 cut(s) 439
MaeII ACGT 7 cut(s) 216, 258, 370, 465, 516, 576, 783
MaeIII GTNAC 1 cut(s) 861
MalI GATC 5 cut(s) 507, 707, 757, 858, 926
MbiI CCGCTC 1 cut(s) 123
MboI GATC 5 cut(s) 505, 705, 755, 856, 924
MboII GAAGA 3 cut(s) 464, 910, 972
MluCI AATT 3 cut(s) 473, 492, 937
MlyI GAGTC 2 cut(s) 806, 986
MmeI TCCRAC 1 cut(s) 237
Mph1103I ATGCAT 2 cut(s) 6, 10
MreI CGCCGGCG 1 cut(s) 554
MroNI GCCGGC 1 cut(s) 554
MroXI GAANNNNTTC 1 cut(s) 1007
MseI TTAA 4 cut(s) 83, 138, 993, 1033
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 6 cut(s) 425, 529, 543, 555, 749, 827
MspR9I CCNGG 4 cut(s) 529, 566, 827, 890
MvaI CCWGG 2 cut(s) 566, 890
MvnI CGCG 2 cut(s) 200, 773
MwoI GCNNNNNNNGC 2 cut(s) 129, 558
NaeI GCCGGC 1 cut(s) 556
NciI CCSGG 2 cut(s) 529, 827
NdeII GATC 5 cut(s) 505, 705, 755, 856, 924
NgoMIV GCCGGC 1 cut(s) 554
NlaIII CATG 7 cut(s) 8, 69, 422, 463, 602, 847, 950
NlaIV GGNNCC 3 cut(s) 228, 248, 767
NsiI ATGCAT 2 cut(s) 6, 10
NspI RCATGY 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 315
PaeI GCATGC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PdiI GCCGGC 1 cut(s) 556
PdmI GAANNNNTTC 1 cut(s) 1007
PfeI GAWTC 2 cut(s) 321, 671
PflMI CCANNNNNTGG 3 cut(s) 577, 650, 775
PkrI GCNGC 2 cut(s) 122, 560
PleI GAGTC 2 cut(s) 805, 985
PmaCI CACGTG 1 cut(s) 577
PmlI CACGTG 1 cut(s) 577
PpsI GAGTC 2 cut(s) 805, 985
Ppu21I YACGTR 1 cut(s) 577
PshAI GACNNNNGTC 1 cut(s) 101
Psp1406I AACGTT 1 cut(s) 516
Psp6I CCWGG 2 cut(s) 564, 888
PspCI CACGTG 1 cut(s) 577
PspGI CCWGG 2 cut(s) 564, 888
PspN4I GGNNCC 3 cut(s) 228, 248, 767
PspPI GGNCC 7 cut(s) 34, 73, 226, 568, 666, 766, 791
RsaI GTAC 2 cut(s) 884, 932
RsaNI GTAC 2 cut(s) 883, 931
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 4 cut(s) 83, 138, 993, 1033
SatI GCNGC 2 cut(s) 121, 559
Sau3AI GATC 5 cut(s) 505, 705, 755, 856, 924
Sau96I GGNCC 7 cut(s) 34, 73, 226, 568, 666, 766, 791
SchI GAGTC 2 cut(s) 806, 986
ScrFI CCNGG 4 cut(s) 529, 566, 827, 890
SfaNI GCATC 1 cut(s) 439
SgrAI CRCCGGYG 1 cut(s) 554
SinI GGWCC 4 cut(s) 73, 226, 666, 766
SmiMI CAYNNNNRTG 1 cut(s) 315
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 473, 492, 937
StyD4I CCNGG 4 cut(s) 527, 564, 825, 888
TaaI ACNGT 3 cut(s) 103, 136, 314
TaiI ACGT 7 cut(s) 219, 261, 373, 468, 519, 579, 786
TaqI TCGA 6 cut(s) 108, 162, 758, 855, 927, 1014
TaqII GACCGA 1 cut(s) 683
TasI AATT 3 cut(s) 473, 492, 937
TatI WGTACW 1 cut(s) 882
TauI GCSGC 2 cut(s) 123, 561
TfiI GAWTC 2 cut(s) 321, 671
Tru1I TTAA 4 cut(s) 83, 138, 993, 1033
Tru9I TTAA 4 cut(s) 83, 138, 993, 1033
TscAI CASTG 1 cut(s) 139
TspDTI ATGAA 4 cut(s) 466, 832, 935, 1000
TspGWI ACGGA 3 cut(s) 65, 394, 849
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 3 cut(s) 577, 650, 775
VpaK11BI GGWCC 4 cut(s) 73, 226, 666, 766
XapI RAATTY 2 cut(s) 473, 937
XceI RCATGY 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 1007
ZraI GACGTC 1 cut(s) 466
Zsp2I ATGCAT 2 cut(s) 6, 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.