Rh7DG040800

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000184
Physical Location & Seq
Forward (+)
2983227 .. 2984406
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG040800.1

Sequence Viewer

Length: 999 bp
ATGGCCAAAATCCAAAGCTCACCAGATCTGCCATGGACCGTCCGCCTCTTCCTCTCCGTCGGTTCCTTCGTAGGCGACGCCTCTCGCCGCTCAAATTTCACTGTCAACCGTCGCCTCTGGAACTTGTTCGACTACAAAACCTCTCCCTTAACAAAACCTCACGGAAACCTCATGAGCTCCGACGTCGTCACCGACCCTTCCCGCAACCTCTGGTTCCGCCTCTACACTCCCACGGCCGCAACCACCAAGCTCCCCATCATCGTCTTCTTCCACGGAGGCGGGTTCGTCTTCATGTCCGCCAGTTCAAAACCCTTCGACGATTTTTGTCAACGCCTTGCTCGGGAGCTCCCCGCCGTCGTCGTCTCCGTCAACTACCGCCTCTCGCCGGAGCATCGATATCCTTGCCAGTACGACGACGGATTCGACGTCCTCAAGTTCATCGACGGCAGCAGCAGCAGCTGCTTGTTCGAGGGCGCCAATCTCGGACAGTGCTTTCTGGCCGGGGACAGCGCCGGCGGGAACATAGCGCACCACGTGGCGATCAGAGCCAGCGGGCACGAGTTCCGGCACGTAAAGGTGGTCGGCATGTTGGCGATTCAGCCGTTTTTCGGCGGAGAAGAGCGGACGGAATCGGAGACGAGACTGAAAAATATGCCGCTGGTGAACATAGAGCGGACGGACTGGATGTGGAAGGCGTTTTTGCCGGAGGGGTCGGATCGGGACCATCCGGTGGCGAATGTGTTCGGGCCGAACGCAGTGGATATATCGGGGGTGGATTTTCCGGCGACGGTTGTGATTGTTGGGGGATTGGATCCGTTGCAAGATTGGCAGAAGAGGTACTACGAGGGATTGAAAAAAAGCGGGAAACAAGCGGAGCTGGTGGTGTTTCCGAATGCAATTCACACTTTCTATGGTTTTCCCGAGTTGGATGACTCTTCTGTGTTTATTCACAAAGTCAGGGATTTCGTACAAGCGCATGCAACTGAAACTCAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

332

Amino Acids

37.15

Weight (kDa)

6.45

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 73 - 128 1.5e-08 Carboxylesterase family
BD-FAE PF20434 74 - 188 1.5e-12 BD-FAE
Abhydrolase_3 PF07859 87 - 304 5.7e-60 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 186, 429
AccB1I GGYRCC 1 cut(s) 473
AccB7I CCANNNNNTGG 1 cut(s) 730
AccBSI CCGCTC 3 cut(s) 90, 622, 673
AclWI GGATC 3 cut(s) 723, 806, 819
AcoI YGGCCR 3 cut(s) 3, 234, 498
AcsI RAATTY 1 cut(s) 94
AcvI CACGTG 1 cut(s) 535
AcyI GRCGYC 4 cut(s) 78, 183, 426, 474
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 3 cut(s) 410, 839, 969
AfiI CCNNNNNNNGG 4 cut(s) 340, 385, 608, 730
AgsI TTSAA 2 cut(s) 306, 853
AluBI AGCT 6 cut(s) 18, 177, 250, 346, 459, 877
AluI AGCT 6 cut(s) 18, 177, 250, 346, 459, 877
Alw21I GWGCWC 2 cut(s) 179, 348
Alw26I GTCTC 3 cut(s) 367, 629, 634
AlwI GGATC 3 cut(s) 723, 806, 819
AlwNI CAGNNNCTG 1 cut(s) 459
Ama87I CYCGRG 2 cut(s) 339, 920
AoxI GGCC 4 cut(s) 3, 234, 498, 746
ApeKI GCWGC 5 cut(s) 447, 450, 453, 456, 459
ApoI RAATTY 1 cut(s) 94
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
Asp700I GAANNNNTTC 2 cut(s) 125, 740
AspLEI GCGC 4 cut(s) 476, 512, 529, 976
AspS9I GGNCC 3 cut(s) 36, 721, 746
AsuC2I CCSGG 1 cut(s) 502
AsuHPI GGTGA 3 cut(s) 12, 181, 673
AvaI CYCGRG 2 cut(s) 339, 920
AvaII GGWCC 2 cut(s) 36, 721
BaeGI GKGCMC 1 cut(s) 558
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 811
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 2 cut(s) 179, 348
BarI GAAGNNNNNNTAC 2 cut(s) 824, 856
BauI CACGAG 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 535
BbsI GAAGAC 2 cut(s) 256, 280
Bbv12I GWGCWC 2 cut(s) 179, 348
BbvI GCAGC 5 cut(s) 446, 459, 462, 465, 468
BccI CCATC 2 cut(s) 263, 732
BceAI ACGGC 4 cut(s) 249, 338, 460, 586
BcgI CGANNNNNNTGC 2 cut(s) 384, 418
BcnI CCSGG 1 cut(s) 502
BcoDI GTCTC 3 cut(s) 367, 629, 634
BfoI RGCGCY 2 cut(s) 477, 513
BglII AGATCT 1 cut(s) 25
BisI GCNGC 8 cut(s) 88, 237, 448, 451, 454, 457, 460, 656
BlsI GCNGC 8 cut(s) 89, 238, 449, 452, 455, 458, 461, 657
Bme1390I CCNGG 1 cut(s) 502
Bme18I GGWCC 2 cut(s) 36, 721
BmeT110I CYCGRG 2 cut(s) 339, 920
BmgT120I GGNCC 3 cut(s) 36, 721, 746
BmiI GGNNCC 5 cut(s) 64, 215, 475, 722, 813
BmrFI CCNGG 1 cut(s) 502
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 2 cut(s) 256, 280
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 502
Bsa29I ATCGAT 1 cut(s) 394
BsaAI YACGTR 2 cut(s) 535, 571
BsaHI GRCGYC 4 cut(s) 78, 183, 426, 474
BsaJI CCNNGG 4 cut(s) 32, 231, 271, 501
BsaWI WCCGGW 1 cut(s) 727
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 385, 608, 730
Bse118I RCCGGY 1 cut(s) 512
Bse1I ACTGG 3 cut(s) 300, 406, 686
BseCI ATCGAT 1 cut(s) 394
BseDI CCNNGG 4 cut(s) 32, 231, 271, 501
BseGI GGATG 3 cut(s) 690, 724, 934
BseLI CCNNNNNNNGG 4 cut(s) 340, 385, 608, 730
BseNI ACTGG 3 cut(s) 300, 406, 686
BseSI GKGCMC 1 cut(s) 558
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 5 cut(s) 446, 459, 462, 465, 468
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 4 cut(s) 5, 236, 500, 748
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 179, 348
BsiHKCI CYCGRG 2 cut(s) 339, 920
BsiSI CCGG 7 cut(s) 386, 501, 513, 565, 704, 728, 782
BslFI GGGAC 2 cut(s) 518, 734
BslI CCNNNNNNNGG 4 cut(s) 340, 385, 608, 730
BsmAI GTCTC 3 cut(s) 367, 629, 634
BsmBI CGTCTC 2 cut(s) 367, 629
BsmFI GGGAC 2 cut(s) 518, 734
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 4 cut(s) 5, 236, 500, 748
BsoBI CYCGRG 2 cut(s) 339, 920
Bsp1286I GDGCHC 3 cut(s) 179, 348, 558
Bsp143I GATC 4 cut(s) 25, 540, 715, 811
Bsp19I CCATGG 1 cut(s) 32
BspANI GGCC 4 cut(s) 5, 236, 500, 748
BspDI ATCGAT 1 cut(s) 394
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 5 cut(s) 64, 215, 475, 722, 813
BspPI GGATC 3 cut(s) 723, 806, 819
BspQI GCTCTTC 1 cut(s) 612
BspT107I GGYRCC 1 cut(s) 473
BsrBI CCGCTC 3 cut(s) 90, 622, 673
BsrFI RCCGGY 1 cut(s) 512
BsrI ACTGG 3 cut(s) 300, 406, 686
BssAI RCCGGY 1 cut(s) 512
BssECI CCNNGG 4 cut(s) 32, 231, 271, 501
BssMI GATC 4 cut(s) 25, 540, 715, 811
BssNI GRCGYC 4 cut(s) 78, 183, 426, 474
BssSI CACGAG 1 cut(s) 557
BssT1I CCWWGG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 557
Bst4CI ACNGT 5 cut(s) 40, 103, 110, 489, 790
Bst6I CTCTTC 4 cut(s) 53, 612, 827, 940
BstACI GRCGYC 4 cut(s) 78, 183, 426, 474
BstAPI GCANNNNNTGC 1 cut(s) 459
BstBAI YACGTR 2 cut(s) 535, 571
BstC8I GCNNGC 4 cut(s) 514, 550, 554, 978
BstDSI CCRYGG 3 cut(s) 32, 231, 271
BstF5I GGATG 3 cut(s) 690, 724, 934
BstH2I RGCGCY 2 cut(s) 477, 513
BstHHI GCGC 4 cut(s) 476, 512, 529, 976
BstKTI GATC 4 cut(s) 28, 543, 718, 814
BstMAI GTCTC 3 cut(s) 367, 629, 634
BstMBI GATC 4 cut(s) 25, 540, 715, 811
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 5 cut(s) 453, 456, 459, 545, 826
BstNSI RCATGY 2 cut(s) 589, 980
BstSCI CCNGG 1 cut(s) 500
BstSLI GKGCMC 1 cut(s) 558
BstV1I GCAGC 5 cut(s) 446, 459, 462, 465, 468
BstV2I GAAGAC 2 cut(s) 256, 280
BstX2I RGATCY 2 cut(s) 25, 811
BstYI RGATCY 2 cut(s) 25, 811
BstZI CGGCCG 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 394
BsuRI GGCC 4 cut(s) 5, 236, 500, 748
BsuTUI ATCGAT 1 cut(s) 394
BtgI CCRYGG 3 cut(s) 32, 231, 271
BtsCI GGATG 3 cut(s) 690, 724, 934
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 99, 494, 762
Cac8I GCNNGC 4 cut(s) 514, 550, 554, 978
CaiI CAGNNNCTG 1 cut(s) 459
CciI TCATGA 1 cut(s) 171
CfoI GCGC 4 cut(s) 476, 512, 529, 976
Cfr10I RCCGGY 1 cut(s) 512
Cfr13I GGNCC 3 cut(s) 36, 721, 746
ClaI ATCGAT 1 cut(s) 394
CseI GACGC 1 cut(s) 86
Csp6I GTAC 3 cut(s) 409, 838, 968
CviAII CATG 5 cut(s) 33, 172, 292, 586, 977
CviQI GTAC 3 cut(s) 409, 838, 968
DinI GGCGCC 1 cut(s) 475
DpnI GATC 4 cut(s) 27, 542, 717, 813
DpnII GATC 4 cut(s) 25, 540, 715, 811
DraIII CACNNNGTG 1 cut(s) 535
EaeI YGGCCR 3 cut(s) 3, 234, 498
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 4 cut(s) 53, 612, 827, 940
EarI CTCTTC 4 cut(s) 53, 612, 827, 940
EciI GGCGGA 4 cut(s) 32, 206, 286, 627
Ecl136II GAGCTC 2 cut(s) 177, 346
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 2 cut(s) 179, 348
Eco32I GATATC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 36, 721
Eco52I CGGCCG 1 cut(s) 234
Eco53kI GAGCTC 2 cut(s) 177, 346
Eco72I CACGTG 1 cut(s) 535
Eco88I CYCGRG 2 cut(s) 339, 920
EcoICRI GAGCTC 2 cut(s) 177, 346
EcoRV GATATC 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 32
EcoT38I GRGCYC 2 cut(s) 179, 348
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 32
Esp3I CGTCTC 2 cut(s) 367, 629
FaeI CATG 5 cut(s) 36, 175, 295, 589, 980
FaqI GGGAC 2 cut(s) 518, 734
FatI CATG 5 cut(s) 32, 171, 291, 585, 976
FauI CCCGC 6 cut(s) 209, 272, 358, 509, 545, 854
Fnu4HI GCNGC 8 cut(s) 88, 237, 448, 451, 454, 457, 460, 656
FokI GGATG 3 cut(s) 697, 711, 941
FriOI GRGCYC 2 cut(s) 179, 348
Fsp4HI GCNGC 8 cut(s) 88, 237, 448, 451, 454, 457, 460, 656
GlaI GCGC 4 cut(s) 475, 511, 528, 975
GluI GCNGC 8 cut(s) 88, 237, 448, 451, 454, 457, 460, 656
HaeII RGCGCY 2 cut(s) 477, 513
HaeIII GGCC 4 cut(s) 5, 236, 500, 748
HapII CCGG 7 cut(s) 386, 501, 513, 565, 704, 728, 782
HgaI GACGC 1 cut(s) 86
HhaI GCGC 4 cut(s) 476, 512, 529, 976
Hin1I GRCGYC 4 cut(s) 78, 183, 426, 474
Hin1II CATG 5 cut(s) 36, 175, 295, 589, 980
Hin6I GCGC 4 cut(s) 474, 510, 527, 974
HinP1I GCGC 4 cut(s) 474, 510, 527, 974
HincII GTYRAC 3 cut(s) 106, 329, 370
HindII GTYRAC 3 cut(s) 106, 329, 370
HinfI GANTC 4 cut(s) 420, 595, 629, 932
HpaII CCGG 7 cut(s) 386, 501, 513, 565, 704, 728, 782
HphI GGTGA 3 cut(s) 12, 181, 673
Hpy166II GTNNAC 4 cut(s) 106, 329, 370, 664
Hpy188I TCNGA 6 cut(s) 181, 485, 545, 634, 715, 891
Hpy188III TCNNGA 5 cut(s) 118, 172, 341, 719, 920
Hpy8I GTNNAC 4 cut(s) 106, 329, 370, 664
HpyAV CCTTC 4 cut(s) 76, 207, 322, 685
HpyCH4III ACNGT 5 cut(s) 40, 103, 110, 489, 790
HpyCH4IV ACGT 4 cut(s) 183, 426, 534, 570
HpyCH4V TGCA 3 cut(s) 820, 896, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 453, 456, 459, 545, 826
HpySE526I ACGT 4 cut(s) 183, 426, 534, 570
Hsp92I GRCGYC 4 cut(s) 78, 183, 426, 474
Hsp92II CATG 5 cut(s) 36, 175, 295, 589, 980
HspAI GCGC 4 cut(s) 474, 510, 527, 974
KasI GGCGCC 1 cut(s) 473
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Kzo9I GATC 4 cut(s) 25, 540, 715, 811
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 6 cut(s) 182, 255, 343, 351, 388, 874
Lsp1109I GCAGC 5 cut(s) 446, 459, 462, 465, 468
LweI GCATC 1 cut(s) 400
MaeII ACGT 4 cut(s) 183, 426, 534, 570
MaeIII GTNAC 1 cut(s) 187
MalI GATC 4 cut(s) 27, 542, 717, 813
MbiI CCGCTC 3 cut(s) 90, 622, 673
MboI GATC 4 cut(s) 25, 540, 715, 811
MboII GAAGA 7 cut(s) 40, 256, 259, 280, 629, 844, 927
MflI RGATCY 2 cut(s) 25, 811
MhlI GDGCHC 3 cut(s) 179, 348, 558
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 94, 897
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 1 cut(s) 926
MmeI TCCRAC 3 cut(s) 204, 693, 906
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 512
MroNI GCCGGC 1 cut(s) 512
MroXI GAANNNNTTC 2 cut(s) 125, 740
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 149, 997
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 459, 552, 658
MspI CCGG 7 cut(s) 386, 501, 513, 565, 704, 728, 782
MspR9I CCNGG 1 cut(s) 502
Mva1269I GAATGC 1 cut(s) 898
MwoI GCNNNNNNNGC 5 cut(s) 453, 456, 459, 545, 826
NaeI GCCGGC 1 cut(s) 514
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 1 cut(s) 502
NcoI CCATGG 1 cut(s) 32
NdeII GATC 4 cut(s) 25, 540, 715, 811
NgoMIV GCCGGC 1 cut(s) 512
NlaIII CATG 5 cut(s) 36, 175, 295, 589, 980
NlaIV GGNNCC 5 cut(s) 64, 215, 475, 722, 813
NmuCI GTSAC 1 cut(s) 187
NspI RCATGY 2 cut(s) 589, 980
PaeI GCATGC 1 cut(s) 980
PagI TCATGA 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 612
PcsI WCGNNNNNNNCGW 5 cut(s) 66, 189, 363, 420, 423
PctI GAATGC 1 cut(s) 898
PdiI GCCGGC 1 cut(s) 514
PdmI GAANNNNTTC 2 cut(s) 125, 740
PfeI GAWTC 3 cut(s) 420, 595, 629
PflFI GACNNNGTC 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 8 cut(s) 89, 238, 449, 452, 455, 458, 461, 657
PleI GAGTC 1 cut(s) 926
PluTI GGCGCC 1 cut(s) 477
PmaCI CACGTG 1 cut(s) 535
PmlI CACGTG 1 cut(s) 535
PpsI GAGTC 1 cut(s) 926
Ppu21I YACGTR 2 cut(s) 535, 571
Psp124BI GAGCTC 2 cut(s) 179, 348
PspCI CACGTG 1 cut(s) 535
PspN4I GGNNCC 5 cut(s) 64, 215, 475, 722, 813
PspPI GGNCC 3 cut(s) 36, 721, 746
PstNI CAGNNNCTG 1 cut(s) 459
PsuI RGATCY 2 cut(s) 25, 811
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 459
RsaI GTAC 3 cut(s) 410, 839, 969
RsaNI GTAC 3 cut(s) 409, 838, 968
SacI GAGCTC 2 cut(s) 179, 348
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 149, 997
SatI GCNGC 8 cut(s) 88, 237, 448, 451, 454, 457, 460, 656
Sau3AI GATC 4 cut(s) 25, 540, 715, 811
Sau96I GGNCC 3 cut(s) 36, 721, 746
SchI GAGTC 1 cut(s) 926
ScrFI CCNGG 1 cut(s) 502
SduI GDGCHC 3 cut(s) 179, 348, 558
SfaNI GCATC 1 cut(s) 400
SfoI GGCGCC 1 cut(s) 475
SgrAI CRCCGGYG 1 cut(s) 512
SinI GGWCC 2 cut(s) 36, 721
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SphI GCATGC 1 cut(s) 980
Sse9I AATT 2 cut(s) 94, 897
SspDI GGCGCC 1 cut(s) 473
SstI GAGCTC 2 cut(s) 179, 348
StyD4I CCNGG 1 cut(s) 500
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 5 cut(s) 40, 103, 110, 489, 790
TaiI ACGT 4 cut(s) 186, 429, 537, 573
TaqI TCGA 6 cut(s) 129, 315, 394, 423, 441, 468
TasI AATT 2 cut(s) 94, 897
TauI GCSGC 3 cut(s) 90, 239, 658
TfiI GAWTC 3 cut(s) 420, 595, 629
Tru1I TTAA 2 cut(s) 149, 997
Tru9I TTAA 2 cut(s) 149, 997
TscAI CASTG 3 cut(s) 106, 494, 762
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 5 cut(s) 447, 450, 453, 456, 459
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 2 cut(s) 280, 427
TspGWI ACGGA 8 cut(s) 46, 177, 288, 355, 432, 641, 692, 804
TspRI CASTG 3 cut(s) 106, 494, 762
Tth111I GACNNNGTC 1 cut(s) 185
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 36, 721
XapI RAATTY 1 cut(s) 94
XceI RCATGY 2 cut(s) 589, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 30
XmnI GAANNNNTTC 2 cut(s) 125, 740
ZraI GACGTC 2 cut(s) 184, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.