pycom05g05160

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000184
Physical Location & Seq
Forward (+)
6135643 .. 6136665
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g05160.1

Sequence Viewer

Length: 1023 bp
ATGACGACGTCGTTGGAATCCAATACTCAAAAGACCGCAAAGTCATGGACTTCTCTCCCCTGGAGGACGCGGGTGTCGTTGTCCCTTCTCTCAAAAGTAACCGACGCCGCACGCCGCGCGGACGGCACTGTCAATCGCCGTCTCATGAACATACTCGACTTCAAGTCCTCAGCCACTCCTGCCGCTCCCGTCAGAGGCGTCACCTCATCCGACGTCACTGTGGACCCCGCTCGCAAACTCCGGTTCCGCCTCTTCGTCCCCCAAACGACGTCGCCGACTCCATCCCATCTTCCCGTCGTCGTATTCTTCCACGGCGGCGGATTCACCTTCCTCAGCCCCGCCTCATTCGCGTACGACGTCGTTTGCCGCAAGTTTGCTCGGAAAATTCCCGCCGTCGTCGTATCTGTCGATTACCGCCTCTGCCCCGAACACCGGTACCCCTCCCAATACGACGACGGGTTCGACGTCCTTACCTTCCTTGACCAAAACGACGACGTCCTCCCCAAAAACGCAGACAGATCTAGAGTCTTCTTCGCCGGGGACAGCGCCGGAGCCAACCTGGCCCACCACGTGGCTGTTCGCGCCGCCGGCGAGAAGGATCGATTCCGGGTCGTGAAACCTGTCGGGTTGATATCGATACAGCCATTTTTGGGGGGAGAAGAGCGGGTCGAGTCTGAGATCCGGCTCCACGGAGCTCCTCTGGTATCGGTGGCCCGAACGGACTGGCTGTGGAAAGTGTTTTTGCCAGACGGGTCGGACCGGGACCACGAGGCGGCGAACCCGAGTGGGCCGAACGCGGTGGACATTGCGGGTCTGGAGTATCCGAATACGCTTGTATTCACAGGGGGATTGGATCCGCTACAAGACTGGCAGAGGAGGTACTACCAGTGGCTGAAGAAATCGGGGAAGGAGGCGAAGCTAATCGAGTATCCAAACATGTGCCACGCGTTTTATGTGTTTCCTGAATTACCTGAGTCTAACCAGCTGGTTAACCAGGTCAAAGATTTCATTGCTTCTGTTTGA

Protein Analysis

341

Amino Acids

37.91

Weight (kDa)

8.87

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 83 - 141 5.5e-07 Carboxylesterase family
Say1_Mug180 PF10340 84 - 225 1.9e-06 Steryl acetyl hydrolase
BD-FAE PF20434 95 - 195 5.5e-12 BD-FAE
Abhydrolase_3 PF07859 100 - 318 9e-59 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 40, 73, 128
AatII GACGTC 6 cut(s) 11, 216, 272, 360, 468, 498
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccB7I CCANNNNNTGG 1 cut(s) 571
AccBSI CCGCTC 3 cut(s) 185, 230, 664
AccII CGCG 7 cut(s) 70, 117, 119, 350, 582, 797, 947
AclWI GGATC 4 cut(s) 606, 673, 848, 861
AcsI RAATTY 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 914
AcvI CACGTG 1 cut(s) 571
AcyI GRCGYC 8 cut(s) 8, 105, 198, 213, 269, 357, 465, 495
AdeI CACNNNGTG 1 cut(s) 571
AfaI GTAC 3 cut(s) 353, 437, 881
AfiI CCNNNNNNNGG 5 cut(s) 194, 432, 571, 650, 772
AflIII ACRYGT 2 cut(s) 936, 945
AgeI ACCGGT 1 cut(s) 432
AgsI TTSAA 1 cut(s) 163
AhdI GACNNNNNGTC 1 cut(s) 163
AjnI CCWGG 3 cut(s) 59, 558, 993
AluBI AGCT 3 cut(s) 695, 919, 985
AluI AGCT 3 cut(s) 695, 919, 985
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 146
AlwI GGATC 4 cut(s) 606, 673, 848, 861
AlwNI CAGNNNCTG 1 cut(s) 892
Ama87I CYCGRG 1 cut(s) 781
AoxI GGCC 3 cut(s) 561, 711, 788
ApoI RAATTY 1 cut(s) 384
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
AsiGI ACCGGT 1 cut(s) 432
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 3 cut(s) 119, 548, 584
AspS9I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
AsuC2I CCSGG 3 cut(s) 538, 608, 761
AsuHPI GGTGA 2 cut(s) 193, 316
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 3 cut(s) 223, 757, 763
BaeI ACNNNNGTAYC 2 cut(s) 419, 452
BamHI GGATCC 1 cut(s) 853
BanI GGYRCC 1 cut(s) 435
BanII GRGCYC 1 cut(s) 697
BauI CACGAG 1 cut(s) 767
BbrPI CACGTG 1 cut(s) 571
BbsI GAAGAC 1 cut(s) 520
Bbv12I GWGCWC 1 cut(s) 697
BbvCI CCTCAGC 2 cut(s) 169, 332
BccI CCATC 2 cut(s) 289, 294
BceAI ACGGC 4 cut(s) 123, 139, 328, 377
BciT130I CCWGG 3 cut(s) 61, 560, 995
BciVI GTATCC 2 cut(s) 831, 939
BcnI CCSGG 3 cut(s) 538, 608, 761
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 549
BfuI GTATCC 2 cut(s) 831, 939
BglI GCCNNNNNGGC 1 cut(s) 560
BglII AGATCT 1 cut(s) 518
BisI GCNGC 7 cut(s) 108, 115, 183, 316, 367, 585, 774
BlsI GCNGC 7 cut(s) 109, 116, 184, 317, 368, 586, 775
Bme1390I CCNGG 6 cut(s) 61, 538, 560, 608, 761, 995
Bme18I GGWCC 3 cut(s) 223, 757, 763
BmeRI GACNNNNNGTC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 781
BmgT120I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
BmiI GGNNCC 7 cut(s) 225, 245, 437, 553, 686, 764, 855
BmrFI CCNGG 6 cut(s) 61, 538, 560, 608, 761, 995
BpiI GAAGAC 1 cut(s) 520
BpmI CTGGAG 2 cut(s) 82, 836
Bpu10I CCTNAGC 2 cut(s) 169, 332
BpuMI CCSGG 3 cut(s) 538, 608, 761
Bsa29I ATCGAT 2 cut(s) 601, 635
BsaAI YACGTR 1 cut(s) 571
BsaHI GRCGYC 8 cut(s) 8, 105, 198, 213, 269, 357, 465, 495
BsaJI CCNNGG 4 cut(s) 59, 310, 537, 688
BsaWI WCCGGW 2 cut(s) 240, 432
Bsc4I CCNNNNNNNGG 5 cut(s) 194, 432, 571, 650, 772
Bse118I RCCGGY 2 cut(s) 432, 587
Bse1I ACTGG 3 cut(s) 728, 872, 886
Bse3DI GCAATG 2 cut(s) 804, 1008
BseBI CCWGG 3 cut(s) 61, 560, 995
BseCI ATCGAT 2 cut(s) 601, 635
BseDI CCNNGG 4 cut(s) 59, 310, 537, 688
BseGI GGATG 2 cut(s) 206, 281
BseLI CCNNNNNNNGG 5 cut(s) 194, 432, 571, 650, 772
BseMI GCAATG 2 cut(s) 804, 1008
BseMII CTCAG 4 cut(s) 183, 346, 666, 963
BseNI ACTGG 3 cut(s) 728, 872, 886
BseRI GAGGAG 2 cut(s) 687, 889
Bsh1236I CGCG 7 cut(s) 70, 117, 119, 350, 582, 797, 947
BshFI GGCC 3 cut(s) 563, 713, 790
BshNI GGYRCC 1 cut(s) 435
BshTI ACCGGT 1 cut(s) 432
BshVI ATCGAT 2 cut(s) 601, 635
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 1 cut(s) 781
BsiSI CCGG 8 cut(s) 241, 433, 537, 549, 588, 607, 682, 760
BsiWI CGTACG 1 cut(s) 351
BslFI GGGAC 4 cut(s) 67, 242, 554, 776
BslI CCNNNNNNNGG 5 cut(s) 194, 432, 571, 650, 772
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 4 cut(s) 67, 242, 554, 776
BsnI GGCC 3 cut(s) 563, 713, 790
BsoBI CYCGRG 1 cut(s) 781
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 4 cut(s) 518, 598, 678, 853
BspANI GGCC 3 cut(s) 563, 713, 790
BspCNI CTCAG 4 cut(s) 182, 345, 667, 964
BspDI ATCGAT 2 cut(s) 601, 635
BspFNI CGCG 7 cut(s) 70, 117, 119, 350, 582, 797, 947
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 7 cut(s) 225, 245, 437, 553, 686, 764, 855
BspPI GGATC 4 cut(s) 606, 673, 848, 861
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 435
BsrBI CCGCTC 3 cut(s) 185, 230, 664
BsrDI GCAATG 2 cut(s) 804, 1008
BsrFI RCCGGY 2 cut(s) 432, 587
BsrI ACTGG 3 cut(s) 728, 872, 886
BssAI RCCGGY 2 cut(s) 432, 587
BssECI CCNNGG 4 cut(s) 59, 310, 537, 688
BssMI GATC 4 cut(s) 518, 598, 678, 853
BssNI GRCGYC 8 cut(s) 8, 105, 198, 213, 269, 357, 465, 495
BssSI CACGAG 1 cut(s) 767
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 3 cut(s) 61, 560, 995
Bst4CI ACNGT 2 cut(s) 130, 220
Bst6I CTCTTC 2 cut(s) 257, 654
BstACI GRCGYC 8 cut(s) 8, 105, 198, 213, 269, 357, 465, 495
BstBAI YACGTR 1 cut(s) 571
BstC8I GCNNGC 3 cut(s) 112, 232, 589
BstDEI CTNAG 4 cut(s) 169, 332, 675, 972
BstDSI CCRYGG 2 cut(s) 310, 688
BstF5I GGATG 2 cut(s) 206, 281
BstFNI CGCG 7 cut(s) 70, 117, 119, 350, 582, 797, 947
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 3 cut(s) 119, 548, 584
BstKTI GATC 4 cut(s) 521, 601, 681, 856
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 4 cut(s) 518, 598, 678, 853
BstMWI GCNNNNNNNGC 7 cut(s) 116, 123, 179, 347, 560, 581, 588
BstNI CCWGG 3 cut(s) 61, 560, 995
BstNSI RCATGY 1 cut(s) 940
BstSCI CCNGG 6 cut(s) 59, 536, 558, 606, 759, 993
BstUI CGCG 7 cut(s) 70, 117, 119, 350, 582, 797, 947
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 3 cut(s) 518, 678, 853
BstYI RGATCY 3 cut(s) 518, 678, 853
Bsu15I ATCGAT 2 cut(s) 601, 635
BsuI GTATCC 2 cut(s) 831, 939
BsuRI GGCC 3 cut(s) 563, 713, 790
BsuTUI ATCGAT 2 cut(s) 601, 635
BtgI CCRYGG 2 cut(s) 310, 688
BtsCI GGATG 2 cut(s) 206, 281
BtsIMutI CAGTG 3 cut(s) 126, 216, 893
Cac8I GCNNGC 3 cut(s) 112, 232, 589
CaiI CAGNNNCTG 1 cut(s) 892
CciI TCATGA 1 cut(s) 144
CfoI GCGC 3 cut(s) 119, 548, 584
Cfr10I RCCGGY 2 cut(s) 432, 587
Cfr13I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
ClaI ATCGAT 2 cut(s) 601, 635
CpoI CGGWCCG 1 cut(s) 757
CseI GACGC 3 cut(s) 76, 113, 187
CsiI ACCWGGT 1 cut(s) 993
Csp6I GTAC 3 cut(s) 352, 436, 880
CspAI ACCGGT 1 cut(s) 432
CspI CGGWCCG 1 cut(s) 757
CviAII CATG 3 cut(s) 45, 145, 937
CviQI GTAC 3 cut(s) 352, 436, 880
DdeI CTNAG 4 cut(s) 169, 332, 675, 972
DpnI GATC 4 cut(s) 520, 600, 680, 855
DpnII GATC 4 cut(s) 518, 598, 678, 853
DraIII CACNNNGTG 1 cut(s) 571
DrdI GACNNNNNNGTC 3 cut(s) 40, 73, 128
DriI GACNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 3 cut(s) 40, 73, 128
Eam1104I CTCTTC 2 cut(s) 257, 654
Eam1105I GACNNNNNGTC 1 cut(s) 163
EarI CTCTTC 2 cut(s) 257, 654
EciI GGCGGA 2 cut(s) 236, 333
Ecl136II GAGCTC 1 cut(s) 695
Eco24I GRGCYC 1 cut(s) 697
Eco32I GATATC 1 cut(s) 633
Eco47I GGWCC 3 cut(s) 223, 757, 763
Eco53kI GAGCTC 1 cut(s) 695
Eco57I CTGAAG 1 cut(s) 914
Eco72I CACGTG 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 781
EcoICRI GAGCTC 1 cut(s) 695
EcoRII CCWGG 3 cut(s) 59, 558, 993
EcoRV GATATC 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 697
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 3 cut(s) 48, 148, 940
FaiI YATR 5 cut(s) 46, 146, 152, 938, 954
FaqI GGGAC 4 cut(s) 67, 242, 554, 776
FatI CATG 3 cut(s) 44, 144, 936
FauI CCCGC 6 cut(s) 63, 235, 346, 397, 657, 802
Fnu4HI GCNGC 7 cut(s) 108, 115, 183, 316, 367, 585, 774
FokI GGATG 2 cut(s) 193, 268
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 7 cut(s) 108, 115, 183, 316, 367, 585, 774
FspBI CTAG 1 cut(s) 522
GlaI GCGC 3 cut(s) 118, 547, 583
GluI GCNGC 7 cut(s) 108, 115, 183, 316, 367, 585, 774
GsuI CTGGAG 2 cut(s) 82, 836
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 3 cut(s) 563, 713, 790
HapII CCGG 8 cut(s) 241, 433, 537, 549, 588, 607, 682, 760
HgaI GACGC 3 cut(s) 76, 113, 187
HhaI GCGC 3 cut(s) 119, 548, 584
Hin1I GRCGYC 8 cut(s) 8, 105, 198, 213, 269, 357, 465, 495
Hin1II CATG 3 cut(s) 48, 148, 940
Hin6I GCGC 3 cut(s) 117, 546, 582
HinP1I GCGC 3 cut(s) 117, 546, 582
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 7 cut(s) 17, 277, 321, 525, 603, 671, 974
HpaI GTTAAC 1 cut(s) 991
HpaII CCGG 8 cut(s) 241, 433, 537, 549, 588, 607, 682, 760
HphI GGTGA 2 cut(s) 193, 316
Hpy166II GTNNAC 3 cut(s) 223, 802, 991
Hpy188I TCNGA 6 cut(s) 194, 211, 381, 676, 757, 825
Hpy188III TCNNGA 5 cut(s) 145, 522, 613, 815, 962
Hpy8I GTNNAC 3 cut(s) 223, 802, 991
HpyAV CCTTC 5 cut(s) 95, 337, 484, 589, 901
HpyCH4III ACNGT 2 cut(s) 130, 220
HpyCH4IV ACGT 7 cut(s) 8, 213, 269, 357, 465, 495, 570
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 123, 179, 347, 560, 581, 588
HpyF3I CTNAG 4 cut(s) 169, 332, 675, 972
HpySE526I ACGT 7 cut(s) 8, 213, 269, 357, 465, 495, 570
Hsp92I GRCGYC 8 cut(s) 8, 105, 198, 213, 269, 357, 465, 495
Hsp92II CATG 3 cut(s) 48, 148, 940
HspAI GCGC 3 cut(s) 117, 546, 582
KpnI GGTACC 1 cut(s) 439
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 4 cut(s) 518, 598, 678, 853
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 5 cut(s) 190, 551, 690, 692, 700
MabI ACCWGGT 1 cut(s) 993
MaeI CTAG 1 cut(s) 522
MaeII ACGT 7 cut(s) 8, 213, 269, 357, 465, 495, 570
MaeIII GTNAC 3 cut(s) 97, 199, 214
MalI GATC 4 cut(s) 520, 600, 680, 855
MbiI CCGCTC 3 cut(s) 185, 230, 664
MboI GATC 4 cut(s) 518, 598, 678, 853
MboII GAAGA 7 cut(s) 244, 281, 298, 520, 523, 671, 907
MflI RGATCY 3 cut(s) 518, 678, 853
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 2 cut(s) 384, 965
MluI ACGCGT 1 cut(s) 945
MlyI GAGTC 4 cut(s) 271, 534, 680, 983
MmeI TCCRAC 2 cut(s) 234, 735
MreI CGCCGGCG 1 cut(s) 587
MroNI GCCGGC 1 cut(s) 587
MseI TTAA 1 cut(s) 990
MspA1I CMGCKG 1 cut(s) 985
MspI CCGG 8 cut(s) 241, 433, 537, 549, 588, 607, 682, 760
MspR9I CCNGG 6 cut(s) 61, 538, 560, 608, 761, 995
MvaI CCWGG 3 cut(s) 61, 560, 995
MvnI CGCG 7 cut(s) 70, 117, 119, 350, 582, 797, 947
MwoI GCNNNNNNNGC 7 cut(s) 116, 123, 179, 347, 560, 581, 588
NaeI GCCGGC 1 cut(s) 589
NciI CCSGG 3 cut(s) 538, 608, 761
NdeII GATC 4 cut(s) 518, 598, 678, 853
NgoMIV GCCGGC 1 cut(s) 587
NlaIII CATG 3 cut(s) 48, 148, 940
NlaIV GGNNCC 7 cut(s) 225, 245, 437, 553, 686, 764, 855
NmuCI GTSAC 2 cut(s) 199, 214
NspI RCATGY 1 cut(s) 940
PagI TCATGA 1 cut(s) 144
PciI ACATGT 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 6 cut(s) 74, 272, 354, 405, 459, 462
PdiI GCCGGC 1 cut(s) 589
PfeI GAWTC 3 cut(s) 17, 321, 603
Pfl23II CGTACG 1 cut(s) 351
PflFI GACNNNGTC 2 cut(s) 7, 494
PflMI CCANNNNNTGG 1 cut(s) 571
PinAI ACCGGT 1 cut(s) 432
PkrI GCNGC 7 cut(s) 109, 116, 184, 317, 368, 586, 775
PleI GAGTC 4 cut(s) 271, 533, 679, 982
PmaCI CACGTG 1 cut(s) 571
PmlI CACGTG 1 cut(s) 571
PpsI GAGTC 4 cut(s) 271, 533, 679, 982
Ppu21I YACGTR 1 cut(s) 571
PscI ACATGT 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 697
Psp6I CCWGG 3 cut(s) 59, 558, 993
PspCI CACGTG 1 cut(s) 571
PspGI CCWGG 3 cut(s) 59, 558, 993
PspLI CGTACG 1 cut(s) 351
PspN4I GGNNCC 7 cut(s) 225, 245, 437, 553, 686, 764, 855
PspPI GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
PstNI CAGNNNCTG 1 cut(s) 892
PsuI RGATCY 3 cut(s) 518, 678, 853
PsyI GACNNNGTC 2 cut(s) 7, 494
PvuII CAGCTG 1 cut(s) 985
RsaI GTAC 3 cut(s) 353, 437, 881
RsaNI GTAC 3 cut(s) 352, 436, 880
Rsr2I CGGWCCG 1 cut(s) 757
RsrII CGGWCCG 1 cut(s) 757
SacI GAGCTC 1 cut(s) 697
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 1 cut(s) 990
SatI GCNGC 7 cut(s) 108, 115, 183, 316, 367, 585, 774
Sau3AI GATC 4 cut(s) 518, 598, 678, 853
Sau96I GGNCC 6 cut(s) 223, 562, 712, 757, 763, 788
SchI GAGTC 4 cut(s) 271, 534, 680, 983
ScrFI CCNGG 6 cut(s) 61, 538, 560, 608, 761, 995
SduI GDGCHC 1 cut(s) 697
SexAI ACCWGGT 1 cut(s) 993
SgrAI CRCCGGYG 1 cut(s) 587
SinI GGWCC 3 cut(s) 223, 757, 763
Sse9I AATT 2 cut(s) 384, 965
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 697
StyD4I CCNGG 6 cut(s) 59, 536, 558, 606, 759, 993
TaaI ACNGT 2 cut(s) 130, 220
TaiI ACGT 7 cut(s) 11, 216, 272, 360, 468, 498, 573
TaqI TCGA 7 cut(s) 156, 408, 462, 601, 635, 669, 924
TasI AATT 2 cut(s) 384, 965
TauI GCSGC 7 cut(s) 110, 117, 185, 318, 369, 587, 776
TfiI GAWTC 3 cut(s) 17, 321, 603
Tru1I TTAA 1 cut(s) 990
Tru9I TTAA 1 cut(s) 990
TscAI CASTG 3 cut(s) 133, 223, 893
TseFI GTSAC 2 cut(s) 199, 214
Tsp45I GTSAC 2 cut(s) 199, 214
TspDTI ATGAA 2 cut(s) 161, 997
TspGWI ACGGA 2 cut(s) 705, 734
TspRI CASTG 3 cut(s) 133, 223, 893
Tth111I GACNNNGTC 2 cut(s) 7, 494
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 3 cut(s) 223, 757, 763
XapI RAATTY 1 cut(s) 384
XbaI TCTAGA 1 cut(s) 521
XceI RCATGY 1 cut(s) 940
XspI CTAG 1 cut(s) 522
ZraI GACGTC 6 cut(s) 9, 214, 270, 358, 466, 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.