Carboxylesterase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 3812, 4649 |
| AatII | GACGTC | 1 cut(s) | 15 |
| Acc16I | TGCGCA | 1 cut(s) | 2098 |
| Acc36I | ACCTGC | 1 cut(s) | 1740 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 4136, 4560 |
| AccI | GTMKAC | 5 cut(s) | 15, 138, 1643, 3031, 4453 |
| AccII | CGCG | 1 cut(s) | 3849 |
| AciI | CCGC | 7 cut(s) | 161, 183, 371, 1893, 3495, 3565, 4480 |
| AclWI | GGATC | 3 cut(s) | 3736, 3874, 5592 |
| AcoI | YGGCCR | 2 cut(s) | 3, 5691 |
| AcuI | CTGAAG | 8 cut(s) | 431, 786, 1202, 2174, 4577, 4628, 4752, 5846 |
| AcyI | GRCGYC | 3 cut(s) | 12, 18, 1085 |
| AdeI | CACNNNGTG | 1 cut(s) | 3524 |
| AfeI | AGCGCT | 1 cut(s) | 2592 |
| AflII | CTTAAG | 1 cut(s) | 3788 |
| AflIII | ACRYGT | 1 cut(s) | 3847 |
| AgeI | ACCGGT | 1 cut(s) | 3831 |
| AjnI | CCWGG | 7 cut(s) | 128, 419, 831, 1015, 1552, 3389, 4017 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 780, 812, 3391, 3423 |
| Alw21I | GWGCWC | 6 cut(s) | 357, 1796, 3064, 4969, 5014, 5058 |
| Alw44I | GTGCAC | 2 cut(s) | 353, 4965 |
| AlwI | GGATC | 3 cut(s) | 3736, 3874, 5592 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 4262, 4316, 4418 |
| Ama87I | CYCGRG | 1 cut(s) | 62 |
| Aor51HI | AGCGCT | 1 cut(s) | 2592 |
| ApaLI | GTGCAC | 2 cut(s) | 353, 4965 |
| AseI | ATTAAT | 3 cut(s) | 527, 971, 4704 |
| AsiGI | ACCGGT | 1 cut(s) | 3831 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1713, 1716, 4155, 4185 |
| AspLEI | GCGC | 4 cut(s) | 1677, 2099, 2593, 5498 |
| AspS9I | GGNCC | 8 cut(s) | 34, 853, 965, 1145, 1306, 1328, 1534, 1681 |
| AsuC2I | CCSGG | 2 cut(s) | 1654, 5862 |
| AsuII | TTCGAA | 1 cut(s) | 5765 |
| AvaI | CYCGRG | 1 cut(s) | 62 |
| AvaII | GGWCC | 5 cut(s) | 34, 853, 1306, 1328, 1534 |
| BaeGI | GKGCMC | 3 cut(s) | 357, 4969, 5215 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 3076, 3109 |
| BalI | TGGCCA | 2 cut(s) | 5, 5693 |
| BanII | GRGCYC | 2 cut(s) | 1796, 3064 |
| BauI | CACGAG | 3 cut(s) | 356, 4674, 5007 |
| BbsI | GAAGAC | 5 cut(s) | 139, 627, 726, 1334, 4755 |
| Bbv12I | GWGCWC | 6 cut(s) | 357, 1796, 3064, 4969, 5014, 5058 |
| BbvCI | CCTCAGC | 4 cut(s) | 603, 1965, 2560, 4494 |
| BceAI | ACGGC | 2 cut(s) | 2406, 3696 |
| BciT130I | CCWGG | 7 cut(s) | 130, 421, 833, 1017, 1554, 3391, 4019 |
| BciVI | GTATCC | 4 cut(s) | 1304, 2509, 3212, 5159 |
| BclI | TGATCA | 4 cut(s) | 1051, 4063, 4084, 4987 |
| BcnI | CCSGG | 2 cut(s) | 1654, 5862 |
| BfmI | CTRYAG | 7 cut(s) | 2129, 2580, 2805, 3275, 4329, 5438, 5717 |
| BfoI | RGCGCY | 1 cut(s) | 2594 |
| BfrI | CTTAAG | 1 cut(s) | 3788 |
| BfuAI | ACCTGC | 1 cut(s) | 1740 |
| BfuI | GTATCC | 4 cut(s) | 1304, 2509, 3212, 5159 |
| BglII | AGATCT | 7 cut(s) | 2737, 2791, 2999, 3871, 4056, 5442, 5565 |
| BlpI | GCTNAGC | 2 cut(s) | 1736, 5013 |
| BmcAI | AGTACT | 1 cut(s) | 2216 |
| Bme1390I | CCNGG | 9 cut(s) | 130, 421, 833, 1017, 1554, 1654, 3391, 4019, 5862 |
| Bme18I | GGWCC | 5 cut(s) | 34, 853, 1306, 1328, 1534 |
| BmeT110I | CYCGRG | 1 cut(s) | 62 |
| BmgT120I | GGNCC | 8 cut(s) | 34, 853, 965, 1145, 1306, 1328, 1534, 1681 |
| BmiI | GGNNCC | 4 cut(s) | 35, 36, 1307, 4128 |
| BmrFI | CCNGG | 9 cut(s) | 130, 421, 833, 1017, 1554, 1654, 3391, 4019, 5862 |
| BmrI | ACTGGG | 4 cut(s) | 159, 1572, 4311, 4847 |
| BmsI | GCATC | 9 cut(s) | 893, 1208, 1521, 2049, 2149, 2965, 3517, 3814, 4686 |
| BmuI | ACTGGG | 4 cut(s) | 159, 1572, 4311, 4847 |
| BpiI | GAAGAC | 5 cut(s) | 139, 627, 726, 1334, 4755 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 4207, 4239 |
| BpmI | CTGGAG | 4 cut(s) | 483, 4052, 4113, 4571 |
| Bpu10I | CCTNAGC | 5 cut(s) | 603, 1965, 2560, 4494, 5547 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1736, 5013 |
| Bpu14I | TTCGAA | 1 cut(s) | 5765 |
| BpuEI | CTTGAG | 5 cut(s) | 305, 2789, 3192, 3575, 4158 |
| BpuMI | CCSGG | 2 cut(s) | 1654, 5862 |
| BsaBI | GATNNNNATC | 1 cut(s) | 3801 |
| BsaHI | GRCGYC | 3 cut(s) | 12, 18, 1085 |
| BsaI | GGTCTC | 1 cut(s) | 321 |
| BsaJI | CCNNGG | 5 cut(s) | 1309, 1552, 1653, 1911, 5188 |
| BsaWI | WCCGGW | 3 cut(s) | 1335, 3831, 4969 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 5507, 5537 |
| Bse118I | RCCGGY | 1 cut(s) | 3831 |
| Bse3DI | GCAATG | 2 cut(s) | 337, 4482 |
| Bse8I | GATNNNNATC | 1 cut(s) | 3801 |
| BseBI | CCWGG | 7 cut(s) | 130, 421, 833, 1017, 1554, 3391, 4019 |
| BseDI | CCNNGG | 5 cut(s) | 1309, 1552, 1653, 1911, 5188 |
| BseJI | GATNNNNATC | 1 cut(s) | 3801 |
| BseMI | GCAATG | 2 cut(s) | 337, 4482 |
| BseRI | GAGGAG | 6 cut(s) | 63, 620, 1295, 2548, 3165, 5528 |
| BseSI | GKGCMC | 3 cut(s) | 357, 4969, 5215 |
| BseYI | CCCAGC | 1 cut(s) | 5130 |
| BsgI | GTGCAG | 4 cut(s) | 2814, 3663, 3966, 4710 |
| Bsh1236I | CGCG | 1 cut(s) | 3849 |
| BshTI | ACCGGT | 1 cut(s) | 3831 |
| BsiHKAI | GWGCWC | 6 cut(s) | 357, 1796, 3064, 4969, 5014, 5058 |
| BsiHKCI | CYCGRG | 1 cut(s) | 62 |
| BsiSI | CCGG | 6 cut(s) | 1336, 1653, 1741, 3832, 4970, 5862 |
| BslFI | GGGAC | 5 cut(s) | 47, 1319, 1535, 3547, 4848 |
| BsmBI | CGTCTC | 3 cut(s) | 1523, 5792, 5826 |
| BsmFI | GGGAC | 5 cut(s) | 47, 1319, 1535, 3547, 4848 |
| BsmI | GAATGC | 3 cut(s) | 1076, 3107, 3567 |
| Bso31I | GGTCTC | 1 cut(s) | 321 |
| BsoBI | CYCGRG | 1 cut(s) | 62 |
| Bsp119I | TTCGAA | 1 cut(s) | 5765 |
| Bsp1286I | GDGCHC | 7 cut(s) | 357, 1796, 3064, 4969, 5014, 5058, 5215 |
| Bsp1407I | TGTACA | 2 cut(s) | 1440, 2384 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1736, 5013 |
| BspACI | CCGC | 7 cut(s) | 161, 183, 371, 1893, 3495, 3565, 4480 |
| BspFNI | CGCG | 1 cut(s) | 3849 |
| BspHI | TCATGA | 1 cut(s) | 4990 |
| BspLI | GGNNCC | 4 cut(s) | 35, 36, 1307, 4128 |
| BspMAI | CTGCAG | 3 cut(s) | 2133, 3279, 5442 |
| BspMI | ACCTGC | 1 cut(s) | 1740 |
| BspPI | GGATC | 3 cut(s) | 3736, 3874, 5592 |
| BspT104I | TTCGAA | 1 cut(s) | 5765 |
| BspTI | CTTAAG | 1 cut(s) | 3788 |
| BspTNI | GGTCTC | 1 cut(s) | 321 |
| BsrDI | GCAATG | 2 cut(s) | 337, 4482 |
| BsrFI | RCCGGY | 1 cut(s) | 3831 |
| BsrGI | TGTACA | 2 cut(s) | 1440, 2384 |
| BssAI | RCCGGY | 1 cut(s) | 3831 |
| BssECI | CCNNGG | 5 cut(s) | 1309, 1552, 1653, 1911, 5188 |
| BssNAI | GTATAC | 2 cut(s) | 1644, 3032 |
| BssNI | GRCGYC | 3 cut(s) | 12, 18, 1085 |
| BssSI | CACGAG | 3 cut(s) | 356, 4674, 5007 |
| BssT1I | CCWWGG | 1 cut(s) | 5188 |
| Bst1107I | GTATAC | 2 cut(s) | 1644, 3032 |
| Bst2BI | CACGAG | 3 cut(s) | 356, 4674, 5007 |
| Bst2UI | CCWGG | 7 cut(s) | 130, 421, 833, 1017, 1554, 3391, 4019 |
| Bst6I | CTCTTC | 3 cut(s) | 158, 2193, 4034 |
| BstACI | GRCGYC | 3 cut(s) | 12, 18, 1085 |
| BstAFI | CTTAAG | 1 cut(s) | 3788 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 3476 |
| BstAUI | TGTACA | 2 cut(s) | 1440, 2384 |
| BstBI | TTCGAA | 1 cut(s) | 5765 |
| BstDSI | CCRYGG | 1 cut(s) | 1911 |
| BstEII | GGTNACC | 1 cut(s) | 1747 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1412, 5240 |
| BstFNI | CGCG | 1 cut(s) | 3849 |
| BstH2I | RGCGCY | 1 cut(s) | 2594 |
| BstHHI | GCGC | 4 cut(s) | 1677, 2099, 2593, 5498 |
| BstNI | CCWGG | 7 cut(s) | 130, 421, 833, 1017, 1554, 3391, 4019 |
| BstNSI | RCATGY | 5 cut(s) | 1550, 1840, 3296, 4601, 5807 |
| BstPI | GGTNACC | 1 cut(s) | 1747 |
| BstSCI | CCNGG | 9 cut(s) | 128, 419, 831, 1015, 1552, 1652, 3389, 4017, 5860 |
| BstSFI | CTRYAG | 7 cut(s) | 2129, 2580, 2805, 3275, 4329, 5438, 5717 |
| BstSLI | GKGCMC | 3 cut(s) | 357, 4969, 5215 |
| BstUI | CGCG | 1 cut(s) | 3849 |
| BstV2I | GAAGAC | 5 cut(s) | 139, 627, 726, 1334, 4755 |
| BstX2I | RGATCY | 7 cut(s) | 2737, 2791, 2999, 3871, 4056, 5442, 5565 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1814, 5701 |
| BstYI | RGATCY | 7 cut(s) | 2737, 2791, 2999, 3871, 4056, 5442, 5565 |
| BstZ17I | GTATAC | 2 cut(s) | 1644, 3032 |
| BsuI | GTATCC | 4 cut(s) | 1304, 2509, 3212, 5159 |
| BtgI | CCRYGG | 1 cut(s) | 1911 |
| BtsI | GCAGTG | 2 cut(s) | 3626, 4371 |
| BveI | ACCTGC | 1 cut(s) | 1740 |
| CaiI | CAGNNNCTG | 3 cut(s) | 4262, 4316, 4418 |
| CciI | TCATGA | 1 cut(s) | 4990 |
| CfoI | GCGC | 4 cut(s) | 1677, 2099, 2593, 5498 |
| Cfr10I | RCCGGY | 1 cut(s) | 3831 |
| Cfr13I | GGNCC | 8 cut(s) | 34, 853, 965, 1145, 1306, 1328, 1534, 1681 |
| CseI | GACGC | 4 cut(s) | 26, 1074, 3083, 5809 |
| CspAI | ACCGGT | 1 cut(s) | 3831 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2952, 2987 |
| DraI | TTTAAA | 3 cut(s) | 939, 3576, 3736 |
| DraIII | CACNNNGTG | 1 cut(s) | 3524 |
| EaeI | YGGCCR | 2 cut(s) | 3, 5691 |
| Eam1104I | CTCTTC | 3 cut(s) | 158, 2193, 4034 |
| EarI | CTCTTC | 3 cut(s) | 158, 2193, 4034 |
| Ecl136II | GAGCTC | 2 cut(s) | 1794, 3062 |
| Eco130I | CCWWGG | 1 cut(s) | 5188 |
| Eco24I | GRGCYC | 2 cut(s) | 1796, 3064 |
| Eco31I | GGTCTC | 1 cut(s) | 321 |
| Eco32I | GATATC | 3 cut(s) | 229, 907, 3227 |
| Eco47I | GGWCC | 5 cut(s) | 34, 853, 1306, 1328, 1534 |
| Eco47III | AGCGCT | 1 cut(s) | 2592 |
| Eco53kI | GAGCTC | 2 cut(s) | 1794, 3062 |
| Eco57I | CTGAAG | 8 cut(s) | 431, 786, 1202, 2174, 4577, 4628, 4752, 5846 |
| Eco88I | CYCGRG | 1 cut(s) | 62 |
| Eco91I | GGTNACC | 1 cut(s) | 1747 |
| EcoICRI | GAGCTC | 2 cut(s) | 1794, 3062 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1412, 5240 |
| EcoO109I | RGGNCCY | 1 cut(s) | 34 |
| EcoO65I | GGTNACC | 1 cut(s) | 1747 |
| EcoRI | GAATTC | 1 cut(s) | 5095 |
| EcoRII | CCWGG | 7 cut(s) | 128, 419, 831, 1015, 1552, 3389, 4017 |
| EcoRV | GATATC | 3 cut(s) | 229, 907, 3227 |
| EcoT14I | CCWWGG | 1 cut(s) | 5188 |
| EcoT38I | GRGCYC | 2 cut(s) | 1796, 3064 |
| ErhI | CCWWGG | 1 cut(s) | 5188 |
| Esp3I | CGTCTC | 3 cut(s) | 1523, 5792, 5826 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 786, 818, 1221, 1253, 1701, 1733, 1704, 1736 |
| FaqI | GGGAC | 5 cut(s) | 47, 1319, 1535, 3547, 4848 |
| FauI | CCCGC | 1 cut(s) | 3572 |
| FauNDI | CATATG | 2 cut(s) | 4091, 5222 |
| FbaI | TGATCA | 4 cut(s) | 1051, 4063, 4084, 4987 |
| FblI | GTMKAC | 5 cut(s) | 15, 138, 1643, 3031, 4453 |
| FriOI | GRGCYC | 2 cut(s) | 1796, 3064 |
| FspI | TGCGCA | 1 cut(s) | 2098 |
| GlaI | GCGC | 4 cut(s) | 1676, 2098, 2592, 5497 |
| GsaI | CCCAGC | 1 cut(s) | 5134 |
| GsuI | CTGGAG | 4 cut(s) | 483, 4052, 4113, 4571 |
| HaeII | RGCGCY | 1 cut(s) | 2594 |
| HapII | CCGG | 6 cut(s) | 1336, 1653, 1741, 3832, 4970, 5862 |
| HgaI | GACGC | 4 cut(s) | 26, 1074, 3083, 5809 |
| HhaI | GCGC | 4 cut(s) | 1677, 2099, 2593, 5498 |
| Hin1I | GRCGYC | 3 cut(s) | 12, 18, 1085 |
| Hin6I | GCGC | 4 cut(s) | 1675, 2097, 2591, 5496 |
| HinP1I | GCGC | 4 cut(s) | 1675, 2097, 2591, 5496 |
| HincII | GTYRAC | 5 cut(s) | 16, 139, 583, 2206, 5278 |
| HindII | GTYRAC | 5 cut(s) | 16, 139, 583, 2206, 5278 |
| HindIII | AAGCTT | 1 cut(s) | 4610 |
| HpaI | GTTAAC | 1 cut(s) | 2206 |
| HpaII | CCGG | 6 cut(s) | 1336, 1653, 1741, 3832, 4970, 5862 |
| Hpy99I | CGWCG | 6 cut(s) | 14, 17, 20, 86, 140, 5797 |
| HpyCH4IV | ACGT | 7 cut(s) | 12, 689, 775, 1421, 2833, 4456, 5403 |
| HpySE526I | ACGT | 7 cut(s) | 12, 689, 775, 1421, 2833, 4456, 5403 |
| Hsp92I | GRCGYC | 3 cut(s) | 12, 18, 1085 |
| HspAI | GCGC | 4 cut(s) | 1675, 2097, 2591, 5496 |
| KflI | GGGWCCC | 1 cut(s) | 34 |
| Ksp22I | TGATCA | 4 cut(s) | 1051, 4063, 4084, 4987 |
| KspAI | GTTAAC | 1 cut(s) | 2206 |
| LmnI | GCTCC | 8 cut(s) | 76, 272, 1384, 1791, 3178, 3588, 3652, 4132 |
| LweI | GCATC | 9 cut(s) | 893, 1208, 1521, 2049, 2149, 2965, 3517, 3814, 4686 |
| MaeII | ACGT | 7 cut(s) | 12, 689, 775, 1421, 2833, 4456, 5403 |
| MfeI | CAATTG | 1 cut(s) | 5592 |
| MflI | RGATCY | 7 cut(s) | 2737, 2791, 2999, 3871, 4056, 5442, 5565 |
| MhlI | GDGCHC | 7 cut(s) | 357, 1796, 3064, 4969, 5014, 5058, 5215 |
| MlsI | TGGCCA | 2 cut(s) | 5, 5693 |
| MluI | ACGCGT | 1 cut(s) | 3847 |
| MluNI | TGGCCA | 2 cut(s) | 5, 5693 |
| MmeI | TCCRAC | 5 cut(s) | 105, 2710, 2914, 4312, 4818 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 5693 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1713, 1716, 4155, 4185 |
| MscI | TGGCCA | 2 cut(s) | 5, 5693 |
| MslI | CAYNNNNRTG | 7 cut(s) | 360, 1040, 1238, 1841, 3525, 4859, 5063 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 5693 |
| MspA1I | CMGCKG | 4 cut(s) | 223, 2458, 4259, 5134 |
| MspCI | CTTAAG | 1 cut(s) | 3788 |
| MspI | CCGG | 6 cut(s) | 1336, 1653, 1741, 3832, 4970, 5862 |
| MspR9I | CCNGG | 9 cut(s) | 130, 421, 833, 1017, 1554, 1654, 3391, 4019, 5862 |
| MunI | CAATTG | 1 cut(s) | 5592 |
| Mva1269I | GAATGC | 3 cut(s) | 1076, 3107, 3567 |
| MvaI | CCWGG | 7 cut(s) | 130, 421, 833, 1017, 1554, 3391, 4019 |
| MvnI | CGCG | 1 cut(s) | 3849 |
| NciI | CCSGG | 2 cut(s) | 1654, 5862 |
| NdeI | CATATG | 2 cut(s) | 4091, 5222 |
| NlaIV | GGNNCC | 4 cut(s) | 35, 36, 1307, 4128 |
| NmeAIII | GCCGAG | 1 cut(s) | 3322 |
| NmuCI | GTSAC | 6 cut(s) | 663, 1284, 2090, 3300, 3426, 3490 |
| NsbI | TGCGCA | 1 cut(s) | 2098 |
| NspI | RCATGY | 5 cut(s) | 1550, 1840, 3296, 4601, 5807 |
| NspV | TTCGAA | 1 cut(s) | 5765 |
| PaeI | GCATGC | 1 cut(s) | 5807 |
| PaeR7I | CTCGAG | 1 cut(s) | 62 |
| PagI | TCATGA | 1 cut(s) | 4990 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 132, 1538, 2004, 2623 |
| PctI | GAATGC | 3 cut(s) | 1076, 3107, 3567 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1713, 1716, 4155, 4185 |
| PflFI | GACNNNGTC | 2 cut(s) | 143, 3424 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 4136, 4560 |
| PfoI | TCCNGGA | 1 cut(s) | 5860 |
| PinAI | ACCGGT | 1 cut(s) | 3831 |
| PpuMI | RGGWCCY | 1 cut(s) | 34 |
| PshBI | ATTAAT | 3 cut(s) | 527, 971, 4704 |
| PsiI | TTATAA | 2 cut(s) | 3812, 4649 |
| Psp124BI | GAGCTC | 2 cut(s) | 1796, 3064 |
| Psp5II | RGGWCCY | 1 cut(s) | 34 |
| Psp6I | CCWGG | 7 cut(s) | 128, 419, 831, 1015, 1552, 3389, 4017 |
| PspEI | GGTNACC | 1 cut(s) | 1747 |
| PspFI | CCCAGC | 1 cut(s) | 5130 |
| PspGI | CCWGG | 7 cut(s) | 128, 419, 831, 1015, 1552, 3389, 4017 |
| PspN4I | GGNNCC | 4 cut(s) | 35, 36, 1307, 4128 |
| PspPI | GGNCC | 8 cut(s) | 34, 853, 965, 1145, 1306, 1328, 1534, 1681 |
| PspPPI | RGGWCCY | 1 cut(s) | 34 |
| PstI | CTGCAG | 3 cut(s) | 2133, 3279, 5442 |
| PstNI | CAGNNNCTG | 3 cut(s) | 4262, 4316, 4418 |
| PsuI | RGATCY | 7 cut(s) | 2737, 2791, 2999, 3871, 4056, 5442, 5565 |
| PsyI | GACNNNGTC | 2 cut(s) | 143, 3424 |
| PvuII | CAGCTG | 4 cut(s) | 223, 2458, 4259, 5134 |
| RseI | CAYNNNNRTG | 7 cut(s) | 360, 1040, 1238, 1841, 3525, 4859, 5063 |
| SacI | GAGCTC | 2 cut(s) | 1796, 3064 |
| SalI | GTCGAC | 2 cut(s) | 14, 137 |
| Sau96I | GGNCC | 8 cut(s) | 34, 853, 965, 1145, 1306, 1328, 1534, 1681 |
| ScaI | AGTACT | 1 cut(s) | 2216 |
| ScrFI | CCNGG | 9 cut(s) | 130, 421, 833, 1017, 1554, 1654, 3391, 4019, 5862 |
| SduI | GDGCHC | 7 cut(s) | 357, 1796, 3064, 4969, 5014, 5058, 5215 |
| SfaNI | GCATC | 9 cut(s) | 893, 1208, 1521, 2049, 2149, 2965, 3517, 3814, 4686 |
| SfcI | CTRYAG | 7 cut(s) | 2129, 2580, 2805, 3275, 4329, 5438, 5717 |
| Sfr274I | CTCGAG | 1 cut(s) | 62 |
| SfuI | TTCGAA | 1 cut(s) | 5765 |
| SgrDI | CGTCGACG | 1 cut(s) | 14 |
| SinI | GGWCC | 5 cut(s) | 34, 853, 1306, 1328, 1534 |
| SlaI | CTCGAG | 1 cut(s) | 62 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 360, 1040, 1238, 1841, 3525, 4859, 5063 |
| SmlI | CTYRAG | 7 cut(s) | 62, 320, 2768, 3207, 3554, 3788, 4173 |
| SmoI | CTYRAG | 7 cut(s) | 62, 320, 2768, 3207, 3554, 3788, 4173 |
| SphI | GCATGC | 1 cut(s) | 5807 |
| SsiI | CCGC | 7 cut(s) | 161, 183, 371, 1893, 3495, 3565, 4480 |
| SstI | GAGCTC | 2 cut(s) | 1796, 3064 |
| StyD4I | CCNGG | 9 cut(s) | 128, 419, 831, 1015, 1552, 1652, 3389, 4017, 5860 |
| StyI | CCWWGG | 1 cut(s) | 5188 |
| TaiI | ACGT | 7 cut(s) | 15, 692, 778, 1424, 2836, 4459, 5406 |
| TaqII | GACCGA | 1 cut(s) | 1323 |
| TatI | WGTACW | 7 cut(s) | 671, 1440, 2164, 2214, 2384, 4043, 5697 |
| TauI | GCSGC | 2 cut(s) | 163, 185 |
| TseFI | GTSAC | 6 cut(s) | 663, 1284, 2090, 3300, 3426, 3490 |
| Tsp45I | GTSAC | 6 cut(s) | 663, 1284, 2090, 3300, 3426, 3490 |
| TspGWI | ACGGA | 3 cut(s) | 1547, 1900, 5810 |
| Tth111I | GACNNNGTC | 2 cut(s) | 143, 3424 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 4136, 4560 |
| Vha464I | CTTAAG | 1 cut(s) | 3788 |
| VneI | GTGCAC | 2 cut(s) | 353, 4965 |
| VpaK11BI | GGWCC | 5 cut(s) | 34, 853, 1306, 1328, 1534 |
| VspI | ATTAAT | 3 cut(s) | 527, 971, 4704 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1412, 5240 |
| XbaI | TCTAGA | 5 cut(s) | 448, 1450, 2476, 3370, 5501 |
| XceI | RCATGY | 5 cut(s) | 1550, 1840, 3296, 4601, 5807 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 5119 |
| XhoI | CTCGAG | 1 cut(s) | 62 |
| XmiI | GTMKAC | 5 cut(s) | 15, 138, 1643, 3031, 4453 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1713, 1716, 4155, 4185 |
| ZraI | GACGTC | 1 cut(s) | 13 |
| ZrmI | AGTACT | 1 cut(s) | 2216 |