Rh7CG433400

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000184
Physical Location & Seq
Reverse (-)
56745056 .. 56745730
675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG433400.1

Sequence Viewer

Length: 675 bp
ATGACGACGTCGTCTCAGTCCCAACAATCCACTCCCTCCTCCTGGGCTTTGCTCCCTTGGAGGACCCGGATCTCGTTATCGCTTCTGTCAAAGTTAACGGACGCCGCTCGCAGACCAAACGGCACCATCAACCGTCGTCTCATGAGCTTCCTCGACTTACGCTCCTCCACCACTCCCTCAGCTCCCTACAAAGGCGTCACCACCTCCGACGTCACCGTCGACCAAACCCGCAACCTCTGGTTTCGCTTCTTCGTCCCCTCTACGACGTCGTCTTCCCTCGCCTCTTTACCCGTCGTCGTTTTCTTCCACGGCGGCGGCTTCAGCTTTCTCAACCCCGACTCCTATGCCTACGACGCCATCTGGGGCAAATTCGCCCGCGAAATCCCTGCCGTCGTAGTCTCCGTCAACTACCGCCTCTCCCCAGATCACGGCTACCCCTGCCAGTACGACGACGGCCTCGACGTCCTCAAATTCCTCGACCAAAACCCCGACTCCCTCCCCGACCTCGCTGACTGCTCCCGCTGCTTCCTCGCCGGAGACAGAGCCGGCGGGAACATTGCCCACCACGTGGCGGTGCGAGCCTGCCGTGAGCCATTTCGGGTCATGAAACCAATCGGGTTGATATCGATCCAACCGTTCTTCGGCGGTGAGGAATGGACCGAGTCCGAGATCTGA

Protein Analysis

224

Amino Acids

24.85

Weight (kDa)

6.89

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 83 - 141 3e-07 Carboxylesterase family
BD-FAE PF20434 94 - 193 6.3e-10 BD-FAE
Abhydrolase_3 PF07859 99 - 216 1.2e-30 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 10, 215
AatII GACGTC 4 cut(s) 11, 213, 269, 465
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 378
AclWI GGATC 2 cut(s) 77, 622
AcsI RAATTY 2 cut(s) 368, 470
AcuI CTGAAG 1 cut(s) 304
AcvI CACGTG 1 cut(s) 568
AcyI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
AdeI CACNNNGTG 1 cut(s) 568
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 6 cut(s) 42, 191, 428, 568, 571, 641
AjnI CCWGG 1 cut(s) 41
AluBI AGCT 3 cut(s) 147, 182, 324
AluI AGCT 3 cut(s) 147, 182, 324
Alw26I GTCTC 4 cut(s) 18, 143, 403, 531
AlwI GGATC 2 cut(s) 77, 622
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 2 cut(s) 368, 470
AspS9I GGNCC 2 cut(s) 63, 657
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 3 cut(s) 190, 205, 659
AvaII GGWCC 2 cut(s) 63, 657
BanI GGYRCC 1 cut(s) 122
BbrPI CACGTG 1 cut(s) 568
BbsI GAAGAC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 509
BccI CCATC 2 cut(s) 134, 365
BceAI ACGGC 6 cut(s) 136, 325, 374, 445, 469, 570
BcgI CGANNNNNNTGC 4 cut(s) 326, 360, 368, 402
BciT130I CCWGG 1 cut(s) 43
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 4 cut(s) 18, 143, 403, 531
BglII AGATCT 1 cut(s) 669
BisI GCNGC 4 cut(s) 105, 313, 316, 523
BlsI GCNGC 4 cut(s) 106, 314, 317, 524
Bme1390I CCNGG 2 cut(s) 43, 67
Bme18I GGWCC 2 cut(s) 63, 657
BmgT120I GGNCC 2 cut(s) 63, 657
BmiI GGNNCC 2 cut(s) 65, 124
BmrFI CCNGG 2 cut(s) 43, 67
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 67
Bsa29I ATCGAT 1 cut(s) 626
BsaAI YACGTR 1 cut(s) 568
BsaBI GATNNNNATC 1 cut(s) 626
BsaHI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
BsaJI CCNNGG 3 cut(s) 42, 56, 307
BsaXI ACNNNNNCTCC 8 cut(s) 146, 176, 323, 353, 401, 431, 476, 506
Bsc4I CCNNNNNNNGG 6 cut(s) 42, 191, 428, 568, 571, 641
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 1 cut(s) 43
BseCI ATCGAT 1 cut(s) 626
BseDI CCNNGG 3 cut(s) 42, 56, 307
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 6 cut(s) 42, 191, 428, 568, 571, 641
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 2 cut(s) 29, 192
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 2 cut(s) 28, 154
BseXI GCAGC 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 456
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 626
BsiSI CCGG 3 cut(s) 67, 534, 546
BslFI GGGAC 2 cut(s) 4, 239
BslI CCNNNNNNNGG 6 cut(s) 42, 191, 428, 568, 571, 641
BsmAI GTCTC 4 cut(s) 18, 143, 403, 531
BsmBI CGTCTC 2 cut(s) 18, 143
BsmFI GGGAC 2 cut(s) 4, 239
BsnI GGCC 1 cut(s) 456
Bsp143I GATC 4 cut(s) 69, 424, 627, 669
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 2 cut(s) 28, 191
BspDI ATCGAT 1 cut(s) 626
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 2 cut(s) 141, 603
BspLI GGNNCC 2 cut(s) 65, 124
BspPI GGATC 2 cut(s) 77, 622
BspT107I GGYRCC 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 1 cut(s) 442
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 3 cut(s) 42, 56, 307
BssMI GATC 4 cut(s) 69, 424, 627, 669
BssNI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
BssT1I CCWWGG 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 3 cut(s) 134, 217, 636
BstACI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
BstBAI YACGTR 1 cut(s) 568
BstC8I GCNNGC 5 cut(s) 109, 376, 547, 579, 583
BstDEI CTNAG 2 cut(s) 15, 178
BstDSI CCRYGG 1 cut(s) 307
BstFNI CGCG 1 cut(s) 378
BstKTI GATC 4 cut(s) 72, 427, 630, 672
BstMAI GTCTC 4 cut(s) 18, 143, 403, 531
BstMBI GATC 4 cut(s) 69, 424, 627, 669
BstMWI GCNNNNNNNGC 5 cut(s) 321, 353, 438, 522, 578
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 41, 65
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 2 cut(s) 69, 669
BstYI RGATCY 2 cut(s) 69, 669
Bsu15I ATCGAT 1 cut(s) 626
BsuRI GGCC 1 cut(s) 456
BsuTUI ATCGAT 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 307
Cac8I GCNNGC 5 cut(s) 109, 376, 547, 579, 583
CciI TCATGA 2 cut(s) 141, 603
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 2 cut(s) 63, 657
ClaI ATCGAT 1 cut(s) 626
CseI GACGC 3 cut(s) 110, 184, 362
Csp6I GTAC 1 cut(s) 445
CviAII CATG 2 cut(s) 142, 604
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 2 cut(s) 15, 178
DpnI GATC 4 cut(s) 71, 426, 629, 671
DpnII GATC 4 cut(s) 69, 424, 627, 669
DraIII CACNNNGTG 1 cut(s) 568
DrdI GACNNNNNNGTC 2 cut(s) 10, 215
DseDI GACNNNNNNGTC 2 cut(s) 10, 215
Eco130I CCWWGG 1 cut(s) 56
Eco32I GATATC 1 cut(s) 624
Eco47I GGWCC 2 cut(s) 63, 657
Eco57I CTGAAG 1 cut(s) 304
Eco72I CACGTG 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 41
EcoRV GATATC 1 cut(s) 624
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
Esp3I CGTCTC 2 cut(s) 18, 143
FaeI CATG 2 cut(s) 145, 607
FaiI YATR 3 cut(s) 143, 345, 605
FaqI GGGAC 2 cut(s) 4, 239
FatI CATG 2 cut(s) 141, 603
FauI CCCGC 4 cut(s) 236, 383, 527, 542
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 4 cut(s) 105, 313, 316, 523
Fsp4HI GCNGC 4 cut(s) 105, 313, 316, 523
GluI GCNGC 4 cut(s) 105, 313, 316, 523
HaeIII GGCC 1 cut(s) 456
HapII CCGG 3 cut(s) 67, 534, 546
HgaI GACGC 3 cut(s) 110, 184, 362
Hin1I GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
Hin1II CATG 2 cut(s) 145, 607
HincII GTYRAC 3 cut(s) 96, 220, 406
HindII GTYRAC 3 cut(s) 96, 220, 406
HinfI GANTC 3 cut(s) 338, 491, 662
HpaI GTTAAC 1 cut(s) 96
HpaII CCGG 3 cut(s) 67, 534, 546
HphI GGTGA 3 cut(s) 190, 205, 659
Hpy166II GTNNAC 3 cut(s) 96, 220, 406
Hpy188I TCNGA 3 cut(s) 208, 667, 674
Hpy188III TCNNGA 2 cut(s) 142, 604
Hpy8I GTNNAC 3 cut(s) 96, 220, 406
HpyCH4III ACNGT 3 cut(s) 134, 217, 636
HpyCH4IV ACGT 5 cut(s) 8, 210, 266, 462, 567
HpyF10VI GCNNNNNNNGC 5 cut(s) 321, 353, 438, 522, 578
HpyF3I CTNAG 2 cut(s) 15, 178
HpySE526I ACGT 5 cut(s) 8, 210, 266, 462, 567
Hsp92I GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
Hsp92II CATG 2 cut(s) 145, 607
KroI GCCGGC 1 cut(s) 545
KroNI GCCGGC 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 96
Kzo9I GATC 4 cut(s) 69, 424, 627, 669
LmnI GCTCC 4 cut(s) 57, 167, 187, 521
Lsp1109I GCAGC 1 cut(s) 509
MaeII ACGT 5 cut(s) 8, 210, 266, 462, 567
MaeIII GTNAC 2 cut(s) 196, 211
MalI GATC 4 cut(s) 71, 426, 629, 671
MbiI CCGCTC 1 cut(s) 107
MboI GATC 4 cut(s) 69, 424, 627, 669
MboII GAAGA 4 cut(s) 241, 264, 295, 631
MflI RGATCY 2 cut(s) 69, 669
MluCI AATT 2 cut(s) 368, 470
MlyI GAGTC 3 cut(s) 332, 485, 671
MmeI TCCRAC 2 cut(s) 231, 655
MroNI GCCGGC 1 cut(s) 545
MseI TTAA 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 522
MspI CCGG 3 cut(s) 67, 534, 546
MspR9I CCNGG 2 cut(s) 43, 67
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 5 cut(s) 321, 353, 438, 522, 578
NaeI GCCGGC 1 cut(s) 547
NciI CCSGG 1 cut(s) 67
NdeII GATC 4 cut(s) 69, 424, 627, 669
NgoMIV GCCGGC 1 cut(s) 545
NlaIII CATG 2 cut(s) 145, 607
NlaIV GGNNCC 2 cut(s) 65, 124
NmuCI GTSAC 2 cut(s) 196, 211
PagI TCATGA 2 cut(s) 141, 603
PcsI WCGNNNNNNNCGW 5 cut(s) 216, 399, 456, 459, 632
PdiI GCCGGC 1 cut(s) 547
PflFI GACNNNGTC 4 cut(s) 7, 10, 268, 661
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 4 cut(s) 106, 314, 317, 524
PleI GAGTC 3 cut(s) 332, 485, 670
PmaCI CACGTG 1 cut(s) 568
PmlI CACGTG 1 cut(s) 568
PpsI GAGTC 3 cut(s) 332, 485, 670
Ppu21I YACGTR 1 cut(s) 568
PpuMI RGGWCCY 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 41
PspCI CACGTG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 65, 124
PspPI GGNCC 2 cut(s) 63, 657
PspPPI RGGWCCY 1 cut(s) 63
PsuI RGATCY 2 cut(s) 69, 669
PsyI GACNNNGTC 4 cut(s) 7, 10, 268, 661
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 4 cut(s) 105, 313, 316, 523
Sau3AI GATC 4 cut(s) 69, 424, 627, 669
Sau96I GGNCC 2 cut(s) 63, 657
SchI GAGTC 3 cut(s) 332, 485, 671
ScrFI CCNGG 2 cut(s) 43, 67
SinI GGWCC 2 cut(s) 63, 657
Sse9I AATT 2 cut(s) 368, 470
StyD4I CCNGG 2 cut(s) 41, 65
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 3 cut(s) 134, 217, 636
TaiI ACGT 5 cut(s) 11, 213, 269, 465, 570
TaqI TCGA 5 cut(s) 153, 219, 459, 477, 626
TaqII GACCGA 1 cut(s) 674
TasI AATT 2 cut(s) 368, 470
TauI GCSGC 3 cut(s) 107, 315, 318
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TseFI GTSAC 2 cut(s) 196, 211
TseI GCWGC 1 cut(s) 522
Tsp45I GTSAC 2 cut(s) 196, 211
TspDTI ATGAA 1 cut(s) 620
TspGWI ACGGA 2 cut(s) 113, 391
Tth111I GACNNNGTC 4 cut(s) 7, 10, 268, 661
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 2 cut(s) 63, 657
XapI RAATTY 2 cut(s) 368, 470
XmiI GTMKAC 1 cut(s) 219
ZraI GACGTC 4 cut(s) 9, 211, 267, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.