Rh5DG438000

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000184
Physical Location & Seq
Forward (+)
69822072 .. 69822683
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG438000.1

Sequence Viewer

Length: 612 bp
ATGAGCTTCCTCGACTTACGCTCCTCCGCCACTCCCTCAGCTCCCTACAAAGGCGTCACCACCTCCGACGTCACCGTCGACCAAACCCGCAACCTCTGGTTCCGCTTCTTCGTCCCCTTTACGACGTCGTCTTCCCCCGCCTCTTTACCCTTCGTCGTTTTCTTCCACGGCGGCAGCTTCAGCTTTCTCAGCCCCGACTCCTACGCCTACGACGCCGTCTGCCGCAAATTCGCTCGCGAAATCCCCGCTGTCGTAGTCTCCGTCAACTACCGCCTCTCCCCAAAGCACGGCTACCCCTGCCAGTACGACGACGGCCTCGACGTCCTCAAATTCCTCGACCAAAACCCCGACTCCCTCCCCGACCTCGCTGACTGCTCTCGCTGCTTCCTCGCCGGAGACAGCGTCGGCGAGAACATTGCCCACCACGTGGCGGTGCGAGCCTGCCGCGAGCCGTTTCGGGTCGTGATATCGATCCAACCGTTCTTCGGCGGCGAGGAATGGACCGAGTCAAAGATCCGACTCGAGGGCGCGCCGCTGGTGTCGGTGACCCGAACCGATTGGCTTTGGAAGGCTTTCTTGCCACAAAACCCAGAAACCCGGTGGCTTCGTTGA

Protein Analysis

203

Amino Acids

22.87

Weight (kDa)

6.07

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 37 - 94 2.6e-06 Carboxylesterase family
BD-FAE PF20434 45 - 146 2e-08 BD-FAE
Abhydrolase_3 PF07859 52 - 197 1.6e-35 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
AatII GACGTC 3 cut(s) 72, 128, 324
AccB7I CCANNNNNTGG 1 cut(s) 427
AccI GTMKAC 1 cut(s) 78
AccII CGCG 3 cut(s) 237, 447, 530
AclWI GGATC 2 cut(s) 466, 508
AcsI RAATTY 2 cut(s) 227, 329
AcuI CTGAAG 1 cut(s) 163
AcvI CACGTG 1 cut(s) 427
AcyI GRCGYC 5 cut(s) 54, 69, 125, 213, 321
AdeI CACNNNGTG 1 cut(s) 427
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 6 cut(s) 50, 287, 427, 430, 485, 523
AluBI AGCT 4 cut(s) 6, 41, 177, 183
AluI AGCT 4 cut(s) 6, 41, 177, 183
Alw26I GTCTC 2 cut(s) 262, 390
AlwI GGATC 2 cut(s) 466, 508
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 2 cut(s) 174, 381
ApoI RAATTY 2 cut(s) 227, 329
AscI GGCGCGCC 1 cut(s) 528
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 2 cut(s) 530, 532
AspS9I GGNCC 1 cut(s) 501
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 3 cut(s) 49, 64, 556
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 123
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 2 cut(s) 186, 368
BceAI ACGGC 5 cut(s) 184, 200, 304, 328, 436
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 2 cut(s) 262, 390
BisI GCNGC 7 cut(s) 172, 175, 223, 382, 445, 490, 533
BlsI GCNGC 7 cut(s) 173, 176, 224, 383, 446, 491, 534
Bme1390I CCNGG 1 cut(s) 598
Bme18I GGWCC 1 cut(s) 501
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 501
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 123
Bpu10I CCTNAGC 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 598
Bsa29I ATCGAT 1 cut(s) 470
BsaAI YACGTR 1 cut(s) 427
BsaBI GATNNNNATC 1 cut(s) 470
BsaHI GRCGYC 5 cut(s) 54, 69, 125, 213, 321
BsaJI CCNNGG 1 cut(s) 166
BsaXI ACNNNNNCTCC 8 cut(s) 5, 35, 260, 290, 335, 365, 387, 417
Bsc4I CCNNNNNNNGG 6 cut(s) 50, 287, 427, 430, 485, 523
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 470
BseCI ATCGAT 1 cut(s) 470
BseDI CCNNGG 1 cut(s) 166
BseJI GATNNNNATC 1 cut(s) 470
BseLI CCNNNNNNNGG 6 cut(s) 50, 287, 427, 430, 485, 523
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 2 cut(s) 51, 202
BseNI ACTGG 1 cut(s) 301
BsePI GCGCGC 1 cut(s) 528
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 2 cut(s) 186, 368
Bsh1236I CGCG 3 cut(s) 237, 447, 530
BshFI GGCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 470
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 2 cut(s) 393, 598
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 6 cut(s) 50, 287, 427, 430, 485, 523
BsmAI GTCTC 2 cut(s) 262, 390
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 1 cut(s) 315
BsoBI CYCGRG 1 cut(s) 521
Bsp143I GATC 2 cut(s) 471, 513
Bsp68I TCGCGA 1 cut(s) 237
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 2 cut(s) 50, 201
BspDI ATCGAT 1 cut(s) 470
BspFNI CGCG 3 cut(s) 237, 447, 530
BspLI GGNNCC 1 cut(s) 101
BspPI GGATC 2 cut(s) 466, 508
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 1 cut(s) 166
BssHII GCGCGC 1 cut(s) 528
BssMI GATC 2 cut(s) 471, 513
BssNI GRCGYC 5 cut(s) 54, 69, 125, 213, 321
Bst4CI ACNGT 2 cut(s) 76, 480
BstACI GRCGYC 5 cut(s) 54, 69, 125, 213, 321
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 5 cut(s) 235, 438, 442, 449, 530
BstDEI CTNAG 2 cut(s) 37, 188
BstDSI CCRYGG 1 cut(s) 166
BstEII GGTNACC 1 cut(s) 544
BstFNI CGCG 3 cut(s) 237, 447, 530
BstHHI GCGC 2 cut(s) 530, 532
BstKTI GATC 2 cut(s) 474, 516
BstMAI GTCTC 2 cut(s) 262, 390
BstMBI GATC 2 cut(s) 471, 513
BstMWI GCNNNNNNNGC 6 cut(s) 180, 189, 212, 297, 381, 437
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 1 cut(s) 596
BstUI CGCG 3 cut(s) 237, 447, 530
BstV1I GCAGC 2 cut(s) 186, 368
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
Bsu15I ATCGAT 1 cut(s) 470
BsuRI GGCC 1 cut(s) 315
BsuTUI ATCGAT 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 166
BtuMI TCGCGA 1 cut(s) 237
Cac8I GCNNGC 5 cut(s) 235, 438, 442, 449, 530
CfoI GCGC 2 cut(s) 530, 532
Cfr13I GGNCC 1 cut(s) 501
ClaI ATCGAT 1 cut(s) 470
CseI GACGC 3 cut(s) 43, 221, 391
Csp6I GTAC 1 cut(s) 304
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 2 cut(s) 37, 188
DpnI GATC 2 cut(s) 473, 515
DpnII GATC 2 cut(s) 471, 513
DraIII CACNNNGTG 1 cut(s) 427
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EciI GGCGGA 1 cut(s) 16
Eco32I GATATC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 501
Eco57I CTGAAG 1 cut(s) 163
Eco72I CACGTG 1 cut(s) 427
Eco88I CYCGRG 1 cut(s) 521
Eco91I GGTNACC 1 cut(s) 544
EcoO65I GGTNACC 1 cut(s) 544
EcoRV GATATC 1 cut(s) 468
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 98
FauI CCCGC 3 cut(s) 95, 145, 253
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 7 cut(s) 172, 175, 223, 382, 445, 490, 533
Fsp4HI GCNGC 7 cut(s) 172, 175, 223, 382, 445, 490, 533
GlaI GCGC 2 cut(s) 529, 531
GluI GCNGC 7 cut(s) 172, 175, 223, 382, 445, 490, 533
HaeIII GGCC 1 cut(s) 315
HapII CCGG 2 cut(s) 393, 598
HgaI GACGC 3 cut(s) 43, 221, 391
HhaI GCGC 2 cut(s) 530, 532
Hin1I GRCGYC 5 cut(s) 54, 69, 125, 213, 321
Hin6I GCGC 2 cut(s) 528, 530
HinP1I GCGC 2 cut(s) 528, 530
HincII GTYRAC 2 cut(s) 79, 265
HindII GTYRAC 2 cut(s) 79, 265
HinfI GANTC 4 cut(s) 197, 350, 506, 519
HpaII CCGG 2 cut(s) 393, 598
HphI GGTGA 3 cut(s) 49, 64, 556
Hpy166II GTNNAC 2 cut(s) 79, 265
Hpy188I TCNGA 2 cut(s) 67, 518
Hpy188III TCNNGA 2 cut(s) 236, 463
Hpy8I GTNNAC 2 cut(s) 79, 265
HpyAV CCTTC 2 cut(s) 160, 562
HpyCH4III ACNGT 2 cut(s) 76, 480
HpyCH4IV ACGT 4 cut(s) 69, 125, 321, 426
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 189, 212, 297, 381, 437
HpyF3I CTNAG 2 cut(s) 37, 188
HpySE526I ACGT 4 cut(s) 69, 125, 321, 426
Hsp92I GRCGYC 5 cut(s) 54, 69, 125, 213, 321
HspAI GCGC 2 cut(s) 528, 530
Kzo9I GATC 2 cut(s) 471, 513
LmnI GCTCC 2 cut(s) 26, 46
LpnPI CCDG 7 cut(s) 82, 310, 314, 406, 454, 521, 603
Lsp1109I GCAGC 2 cut(s) 186, 368
MaeII ACGT 4 cut(s) 69, 125, 321, 426
MaeIII GTNAC 3 cut(s) 55, 70, 544
MalI GATC 2 cut(s) 473, 515
MboI GATC 2 cut(s) 471, 513
MboII GAAGA 4 cut(s) 100, 123, 154, 475
MflI RGATCY 1 cut(s) 513
MluCI AATT 2 cut(s) 227, 329
MlyI GAGTC 4 cut(s) 191, 344, 513, 515
MmeI TCCRAC 3 cut(s) 90, 499, 541
MroXI GAANNNNTTC 1 cut(s) 572
MspA1I CMGCKG 2 cut(s) 248, 535
MspI CCGG 2 cut(s) 393, 598
MspR9I CCNGG 1 cut(s) 598
MvnI CGCG 3 cut(s) 237, 447, 530
MwoI GCNNNNNNNGC 6 cut(s) 180, 189, 212, 297, 381, 437
NciI CCSGG 1 cut(s) 598
NdeII GATC 2 cut(s) 471, 513
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 3 cut(s) 55, 70, 544
NruI TCGCGA 1 cut(s) 237
PaeR7I CTCGAG 1 cut(s) 521
PalAI GGCGCGCC 1 cut(s) 528
PauI GCGCGC 1 cut(s) 528
PcsI WCGNNNNNNNCGW 5 cut(s) 75, 258, 315, 318, 476
PdmI GAANNNNTTC 1 cut(s) 572
PflFI GACNNNGTC 4 cut(s) 127, 215, 401, 505
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 7 cut(s) 173, 176, 224, 383, 446, 491, 534
PleI GAGTC 4 cut(s) 191, 344, 513, 514
PmaCI CACGTG 1 cut(s) 427
PmlI CACGTG 1 cut(s) 427
PpsI GAGTC 4 cut(s) 191, 344, 513, 514
Ppu21I YACGTR 1 cut(s) 427
PspCI CACGTG 1 cut(s) 427
PspEI GGTNACC 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 501
PspXI VCTCGAGB 1 cut(s) 521
PsuI RGATCY 1 cut(s) 513
PsyI GACNNNGTC 4 cut(s) 127, 215, 401, 505
PteI GCGCGC 1 cut(s) 528
RruI TCGCGA 1 cut(s) 237
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
SalI GTCGAC 1 cut(s) 77
SatI GCNGC 7 cut(s) 172, 175, 223, 382, 445, 490, 533
Sau3AI GATC 2 cut(s) 471, 513
Sau96I GGNCC 1 cut(s) 501
SchI GAGTC 4 cut(s) 191, 344, 513, 515
ScrFI CCNGG 1 cut(s) 598
Sfr274I CTCGAG 1 cut(s) 521
SgsI GGCGCGCC 1 cut(s) 528
SinI GGWCC 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 521
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 2 cut(s) 227, 329
StyD4I CCNGG 1 cut(s) 596
TaaI ACNGT 2 cut(s) 76, 480
TaiI ACGT 4 cut(s) 72, 128, 324, 429
TaqI TCGA 6 cut(s) 12, 78, 318, 336, 470, 522
TaqII GACCGA 1 cut(s) 518
TasI AATT 2 cut(s) 227, 329
TauI GCSGC 5 cut(s) 174, 225, 447, 492, 535
TseFI GTSAC 3 cut(s) 55, 70, 544
TseI GCWGC 2 cut(s) 174, 381
Tsp45I GTSAC 3 cut(s) 55, 70, 544
TspGWI ACGGA 1 cut(s) 250
Tth111I GACNNNGTC 4 cut(s) 127, 215, 401, 505
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 1 cut(s) 501
XapI RAATTY 2 cut(s) 227, 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 597
XhoI CTCGAG 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 572
ZraI GACGTC 3 cut(s) 70, 126, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.