Rw5G032500

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000184
Physical Location & Seq
Reverse (-)
55052387 .. 55053378
992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032500.1

Sequence Viewer

Length: 945 bp
ATGACGACGACATCGGCGCCACAACTTCCATGCAAGCTCCGCTTCTTACTCTTCATCCTCTCCATCCTCCTCGACGCAGCTCGCCGCCACAACGGCACCGTCAACCGCCGTCTCATGTCCCTCTTCGACTTCAAGGCCTCTCCTTCACCCAAACCCACCAACCGCGTCAAAACCTCCGATGTAATGGTCGACCACTCCCGTAACCTCTGGTTCCGCTTCTACATCCCCACCAGCACCGACGCCGCCAAACTCCCCGTCATAATATACTTCCATGGTGGCGGTTTTGTCTTCTTCTCTGCCAACTCAAAACCCTACGACGACCTATGCAAGCGCCTTGCCCTCGAGCTCCCAGCCGTCGTCATCTCCGTCAACTACCGCCTCGCGCCGGAGCATCCGTACCCGGCGCAATACGACGACGGGTTCGACGTCCTGAAATTTCCGTGTTTTCTCGCCGGAGATAGCGCCGGCGGCAACGTGGCTCACCACGTGGCAGTAAAGGCCAGTGATCACGAGTTTTCCAAAATGAAGGTTATTGGTGTTATAGGGATACAACCGTTTTTTGGAGGGGAAGAGAGGACTGAATCAGAAACCAAGCTGACCAAGGCTCTTTTAATTACTCTGGAGCGAACCGACTGGTACTGGAAGGCGCTTTTGCCGGAAGGGACGGATAGAGACCACCCAGTGGTTCATGTATTCGGTCCACAGTCAGATGGTGTTTTGGGGAAGAAGTTTCCGGCGACGATCGTTTTCATGGGAGGGTTTGATCCTTTACAAGACTGGCAAAAGAGATACTACGAGGGGTTGAAGAAAGCTGGGAAAGAAGCTTACTTGGTCGAGTACCCGAACTCGTTTCATGGGTTTTACTGTTTTCCTGAACTAAAGGAGTCTTCATTATTAATTGAGGAGGTGAAAGATTTCATCGAGAAGCAATCAGCTCCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

314

Amino Acids

35.47

Weight (kDa)

7.71

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 73 - 169 7.9e-08 BD-FAE
COesterase PF00135 73 - 128 1.1e-07 Carboxylesterase family
Abhydrolase_3 PF07859 87 - 288 1.7e-55 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 429
AccB1I GGYRCC 2 cut(s) 16, 95
AccB7I CCANNNNNTGG 1 cut(s) 682
AccI GTMKAC 1 cut(s) 189
AccII CGCG 2 cut(s) 165, 383
AciI CCGC 9 cut(s) 40, 85, 106, 163, 214, 243, 279, 376, 468
AclWI GGATC 1 cut(s) 758
AcsI RAATTY 1 cut(s) 434
AcvI CACGTG 1 cut(s) 487
AcyI GRCGYC 3 cut(s) 17, 240, 426
AdeI CACNNNGTG 2 cut(s) 487, 682
AfaI GTAC 3 cut(s) 398, 638, 839
AfiI CCNNNNNNNGG 3 cut(s) 385, 560, 682
AgsI TTSAA 2 cut(s) 133, 805
AluBI AGCT 7 cut(s) 37, 80, 346, 595, 812, 824, 935
AluI AGCT 7 cut(s) 37, 80, 346, 595, 812, 824, 935
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 116, 666
AlwI GGATC 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 2 cut(s) 135, 498
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 1 cut(s) 434
ArsI GACNNNNNNTTYG 4 cut(s) 240, 272, 404, 436
AseI ATTAAT 1 cut(s) 896
Asp700I GAANNNNTTC 1 cut(s) 914
AspLEI GCGC 6 cut(s) 19, 333, 385, 406, 464, 649
AspS9I GGNCC 1 cut(s) 698
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 138, 473, 919
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 1 cut(s) 698
BaeI ACNNNNGTAYC 2 cut(s) 539, 572
BanI GGYRCC 2 cut(s) 16, 95
BanII GRGCYC 1 cut(s) 348
BauI CACGAG 1 cut(s) 509
BbrPI CACGTG 1 cut(s) 487
BbsI GAAGAC 2 cut(s) 280, 879
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 89
BccI CCATC 2 cut(s) 71, 704
BceAI ACGGC 3 cut(s) 93, 109, 338
BciVI GTATCC 1 cut(s) 540
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 2 cut(s) 116, 666
BfoI RGCGCY 4 cut(s) 20, 334, 465, 650
BfuI GTATCC 1 cut(s) 540
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 4 cut(s) 78, 85, 243, 469
BlsI GCNGC 4 cut(s) 79, 86, 244, 470
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 1 cut(s) 698
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 698
BmiI GGNNCC 3 cut(s) 18, 97, 212
BmrFI CCNGG 1 cut(s) 401
BmrI ACTGGG 1 cut(s) 674
BmsI GCATC 1 cut(s) 400
BmuI ACTGGG 1 cut(s) 674
BpiI GAAGAC 2 cut(s) 280, 879
BpmI CTGGAG 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 487
BsaHI GRCGYC 3 cut(s) 17, 240, 426
BsaI GGTCTC 1 cut(s) 666
BsaJI CCNNGG 2 cut(s) 271, 600
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 560, 682
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 5 cut(s) 501, 638, 644, 680, 782
BseDI CCNNGG 2 cut(s) 271, 600
BseGI GGATG 4 cut(s) 54, 63, 222, 391
BseLI CCNNNNNNNGG 3 cut(s) 385, 560, 682
BseNI ACTGG 5 cut(s) 501, 638, 644, 680, 782
BseRI GAGGAG 3 cut(s) 59, 917, 927
BseXI GCAGC 1 cut(s) 89
BseYI CCCAGC 2 cut(s) 349, 812
Bsh1236I CGCG 2 cut(s) 165, 383
Bsh1285I CGRYCG 1 cut(s) 744
BshFI GGCC 2 cut(s) 137, 500
BshNI GGYRCC 2 cut(s) 16, 95
BsiEI CGRYCG 1 cut(s) 744
BsiHKAI GWGCWC 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 6 cut(s) 386, 401, 453, 465, 656, 734
BslFI GGGAC 2 cut(s) 103, 676
BslI CCNNNNNNNGG 3 cut(s) 385, 560, 682
BsmAI GTCTC 2 cut(s) 116, 666
BsmBI CGTCTC 1 cut(s) 116
BsmFI GGGAC 2 cut(s) 103, 676
BsnI GGCC 2 cut(s) 137, 500
Bso31I GGTCTC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 3 cut(s) 505, 741, 763
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 9 cut(s) 40, 85, 106, 163, 214, 243, 279, 376, 468
BspANI GGCC 2 cut(s) 137, 500
BspFNI CGCG 2 cut(s) 165, 383
BspLI GGNNCC 3 cut(s) 18, 97, 212
BspPI GGATC 1 cut(s) 758
BspT107I GGYRCC 2 cut(s) 16, 95
BspTNI GGTCTC 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 5 cut(s) 501, 638, 644, 680, 782
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 2 cut(s) 271, 600
BssMI GATC 3 cut(s) 505, 741, 763
BssNI GRCGYC 3 cut(s) 17, 240, 426
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 2 cut(s) 271, 600
Bst2BI CACGAG 1 cut(s) 509
Bst4CI ACNGT 4 cut(s) 100, 555, 705, 866
Bst6I CTCTTC 3 cut(s) 56, 128, 564
BstACI GRCGYC 3 cut(s) 17, 240, 426
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 4 cut(s) 35, 82, 329, 466
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 4 cut(s) 54, 63, 222, 391
BstFNI CGCG 2 cut(s) 165, 383
BstH2I RGCGCY 4 cut(s) 20, 334, 465, 650
BstHHI GCGC 6 cut(s) 19, 333, 385, 406, 464, 649
BstKTI GATC 3 cut(s) 508, 744, 766
BstMAI GTCTC 2 cut(s) 116, 666
BstMBI GATC 3 cut(s) 505, 741, 763
BstMCI CGRYCG 1 cut(s) 744
BstMWI GCNNNNNNNGC 4 cut(s) 39, 93, 468, 497
BstSCI CCNGG 1 cut(s) 399
BstUI CGCG 2 cut(s) 165, 383
BstV1I GCAGC 1 cut(s) 89
BstV2I GAAGAC 2 cut(s) 280, 879
BsuI GTATCC 1 cut(s) 540
BsuRI GGCC 2 cut(s) 137, 500
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 4 cut(s) 54, 63, 222, 391
BtsIMutI CAGTG 2 cut(s) 508, 687
Cac8I GCNNGC 4 cut(s) 35, 82, 329, 466
CfoI GCGC 6 cut(s) 19, 333, 385, 406, 464, 649
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 698
CseI GACGC 3 cut(s) 83, 154, 248
Csp6I GTAC 3 cut(s) 397, 637, 838
CviAII CATG 6 cut(s) 30, 115, 272, 689, 751, 854
CviQI GTAC 3 cut(s) 397, 637, 838
DinI GGCGCC 1 cut(s) 18
DpnI GATC 3 cut(s) 507, 743, 765
DpnII GATC 3 cut(s) 505, 741, 763
DraIII CACNNNGTG 2 cut(s) 487, 682
Eam1104I CTCTTC 3 cut(s) 56, 128, 564
EarI CTCTTC 3 cut(s) 56, 128, 564
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 2 cut(s) 271, 600
Eco147I AGGCCT 1 cut(s) 137
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 698
Eco53kI GAGCTC 1 cut(s) 346
Eco72I CACGTG 1 cut(s) 487
Eco88I CYCGRG 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 346
EcoT14I CCWWGG 2 cut(s) 271, 600
EcoT38I GRGCYC 1 cut(s) 348
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
ErhI CCWWGG 2 cut(s) 271, 600
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 6 cut(s) 33, 118, 275, 692, 754, 857
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FaqI GGGAC 2 cut(s) 103, 676
FatI CATG 6 cut(s) 29, 114, 271, 688, 750, 853
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 4 cut(s) 78, 85, 243, 469
FokI GGATG 4 cut(s) 41, 50, 209, 378
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 4 cut(s) 78, 85, 243, 469
GlaI GCGC 6 cut(s) 18, 332, 384, 405, 463, 648
GluI GCNGC 4 cut(s) 78, 85, 243, 469
GsaI CCCAGC 2 cut(s) 353, 816
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 4 cut(s) 20, 334, 465, 650
HaeIII GGCC 2 cut(s) 137, 500
HapII CCGG 6 cut(s) 386, 401, 453, 465, 656, 734
HgaI GACGC 3 cut(s) 83, 154, 248
HhaI GCGC 6 cut(s) 19, 333, 385, 406, 464, 649
Hin1I GRCGYC 3 cut(s) 17, 240, 426
Hin1II CATG 6 cut(s) 33, 118, 275, 692, 754, 857
Hin6I GCGC 6 cut(s) 17, 331, 383, 404, 462, 647
HinP1I GCGC 6 cut(s) 17, 331, 383, 404, 462, 647
HincII GTYRAC 3 cut(s) 103, 190, 370
HindII GTYRAC 3 cut(s) 103, 190, 370
HindIII AAGCTT 1 cut(s) 822
HinfI GANTC 2 cut(s) 581, 884
HpaII CCGG 6 cut(s) 386, 401, 453, 465, 656, 734
HphI GGTGA 3 cut(s) 138, 473, 919
Hpy166II GTNNAC 4 cut(s) 103, 190, 370, 701
Hpy188I TCNGA 3 cut(s) 178, 586, 709
Hpy188III TCNNGA 5 cut(s) 430, 509, 620, 872, 922
Hpy8I GTNNAC 4 cut(s) 103, 190, 370, 701
Hpy99I CGWCG 9 cut(s) 10, 77, 242, 320, 359, 416, 419, 428, 742
HpyAV CCTTC 4 cut(s) 153, 520, 637, 653
HpyCH4III ACNGT 4 cut(s) 100, 555, 705, 866
HpyCH4IV ACGT 3 cut(s) 426, 474, 486
HpyCH4V TGCA 2 cut(s) 33, 327
HpyF10VI GCNNNNNNNGC 4 cut(s) 39, 93, 468, 497
HpySE526I ACGT 3 cut(s) 426, 474, 486
Hsp92I GRCGYC 3 cut(s) 17, 240, 426
Hsp92II CATG 6 cut(s) 33, 118, 275, 692, 754, 857
HspAI GCGC 6 cut(s) 17, 331, 383, 404, 462, 647
KasI GGCGCC 1 cut(s) 16
KroI GCCGGC 1 cut(s) 464
KroNI GCCGGC 1 cut(s) 466
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 3 cut(s) 505, 741, 763
LmnI GCTCC 5 cut(s) 42, 351, 388, 622, 940
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 1 cut(s) 400
MaeII ACGT 3 cut(s) 426, 474, 486
MaeIII GTNAC 1 cut(s) 200
MalI GATC 3 cut(s) 507, 743, 765
MboI GATC 3 cut(s) 505, 741, 763
MboII GAAGA 8 cut(s) 43, 115, 280, 283, 581, 736, 817, 879
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 3 cut(s) 434, 612, 897
Mly113I GGCGCC 1 cut(s) 17
MlyI GAGTC 1 cut(s) 893
MreI CGCCGGCG 1 cut(s) 464
MroNI GCCGGC 1 cut(s) 464
MroXI GAANNNNTTC 1 cut(s) 914
MseI TTAA 2 cut(s) 611, 896
MspI CCGG 6 cut(s) 386, 401, 453, 465, 656, 734
MspR9I CCNGG 1 cut(s) 401
MvnI CGCG 2 cut(s) 165, 383
MwoI GCNNNNNNNGC 4 cut(s) 39, 93, 468, 497
NaeI GCCGGC 1 cut(s) 466
NarI GGCGCC 1 cut(s) 17
NciI CCSGG 1 cut(s) 401
NcoI CCATGG 1 cut(s) 271
NdeII GATC 3 cut(s) 505, 741, 763
NgoMIV GCCGGC 1 cut(s) 464
NlaIII CATG 6 cut(s) 33, 118, 275, 692, 754, 857
NlaIV GGNNCC 3 cut(s) 18, 97, 212
PaeR7I CTCGAG 1 cut(s) 341
PceI AGGCCT 1 cut(s) 137
PcsI WCGNNNNNNNCGW 3 cut(s) 363, 420, 423
PdiI GCCGGC 1 cut(s) 466
PdmI GAANNNNTTC 1 cut(s) 914
PfeI GAWTC 1 cut(s) 581
PflMI CCANNNNNTGG 1 cut(s) 682
PkrI GCNGC 4 cut(s) 79, 86, 244, 470
Ple19I CGATCG 1 cut(s) 744
PleI GAGTC 1 cut(s) 892
PluTI GGCGCC 1 cut(s) 20
PmaCI CACGTG 1 cut(s) 487
PmlI CACGTG 1 cut(s) 487
PpsI GAGTC 1 cut(s) 892
Ppu21I YACGTR 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 896
Psp124BI GAGCTC 1 cut(s) 348
PspCI CACGTG 1 cut(s) 487
PspFI CCCAGC 2 cut(s) 349, 812
PspN4I GGNNCC 3 cut(s) 18, 97, 212
PspPI GGNCC 1 cut(s) 698
PspXI VCTCGAGB 1 cut(s) 341
PvuI CGATCG 1 cut(s) 744
RsaI GTAC 3 cut(s) 398, 638, 839
RsaNI GTAC 3 cut(s) 397, 637, 838
SacI GAGCTC 1 cut(s) 348
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 611, 896
SatI GCNGC 4 cut(s) 78, 85, 243, 469
Sau3AI GATC 3 cut(s) 505, 741, 763
Sau96I GGNCC 1 cut(s) 698
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 400
SfoI GGCGCC 1 cut(s) 18
Sfr274I CTCGAG 1 cut(s) 341
SgrAI CRCCGGYG 1 cut(s) 464
SinI GGWCC 1 cut(s) 698
SlaI CTCGAG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 3 cut(s) 434, 612, 897
SseBI AGGCCT 1 cut(s) 137
SsiI CCGC 9 cut(s) 40, 85, 106, 163, 214, 243, 279, 376, 468
SspDI GGCGCC 1 cut(s) 16
SstI GAGCTC 1 cut(s) 348
StuI AGGCCT 1 cut(s) 137
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 2 cut(s) 271, 600
TaaI ACNGT 4 cut(s) 100, 555, 705, 866
TaiI ACGT 3 cut(s) 429, 477, 489
TaqI TCGA 7 cut(s) 72, 126, 189, 342, 423, 834, 921
TaqII GACCGA 1 cut(s) 686
TasI AATT 3 cut(s) 434, 612, 897
TauI GCSGC 3 cut(s) 87, 245, 471
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 2 cut(s) 611, 896
Tru9I TTAA 2 cut(s) 611, 896
TscAI CASTG 2 cut(s) 508, 687
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 7 cut(s) 43, 539, 677, 739, 842, 879, 907
TspGWI ACGGA 4 cut(s) 355, 384, 429, 680
TspRI CASTG 2 cut(s) 508, 687
Van91I CCANNNNNTGG 1 cut(s) 682
VpaK11BI GGWCC 1 cut(s) 698
VspI ATTAAT 1 cut(s) 896
XapI RAATTY 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 341
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 914
ZraI GACGTC 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.