Rh6BG005100

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000184
Physical Location & Seq
Reverse (-)
753047 .. 753487
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG005100.1

Sequence Viewer

Length: 441 bp
ATGTCCGCCACTTCAAAACCCTACGACGACTTCTGTCAACGCCTCGCTCTCCAGCTCCCCGCCGTCGTCGTCTCCTTCAACTACCGCCTCGCGCCGGAGCATCGGTATCCGTACCAGTACGACGACGGATTCGACGTCCTCAAGTTCATCGACAACAGTCAAAGCAGCTTGTTCGAGGGCGCGAATCTCGGGCAGTGCTTTCTGGCCGGGGACAGCGCCGGCGGGAACATAGCGCACCACGTGGCGATCAGAGCGAGCGGGCACGAGTTTCGGGATCTGAAAGTGGTCAGAATGTTGGCGATTCAGCCGTTTTTCGGCGGAGAAGAGCGGACAGAATCGGAGACGAGACTGAAGACAGTGCCGCTGGTGAACGTAGAGCGGACGGACTGGATGTGGAAGGCGTTTTTGCCGGTGGGGTCGGATCGGGACCACCCGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

146

Amino Acids

16.58

Weight (kDa)

5.68

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 6 - 146 1.3e-35 alpha/beta hydrolase fold
BD-FAE PF20434 22 - 90 6.7e-06 BD-FAE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccBSI CCGCTC 3 cut(s) 258, 328, 379
AccII CGCG 2 cut(s) 92, 182
AciI CCGC 9 cut(s) 6, 60, 85, 222, 258, 318, 328, 362, 379
AclWI GGATC 2 cut(s) 282, 429
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 1 cut(s) 371
AcvI CACGTG 1 cut(s) 241
AcyI GRCGYC 1 cut(s) 135
AdeI CACNNNGTG 1 cut(s) 241
AfaI GTAC 2 cut(s) 113, 119
AfiI CCNNNNNNNGG 2 cut(s) 94, 314
AgsI TTSAA 2 cut(s) 15, 79
AluBI AGCT 2 cut(s) 55, 168
AluI AGCT 2 cut(s) 55, 168
Alw26I GTCTC 3 cut(s) 76, 335, 340
AlwI GGATC 2 cut(s) 282, 429
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 165
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AspLEI GCGC 4 cut(s) 94, 182, 218, 235
AspS9I GGNCC 1 cut(s) 427
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 427
BaeGI GKGCMC 1 cut(s) 264
BauI CACGAG 1 cut(s) 263
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 177
BceAI ACGGC 2 cut(s) 47, 292
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 251, 285
BciVI GTATCC 1 cut(s) 117
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 3 cut(s) 76, 335, 340
BfoI RGCGCY 1 cut(s) 219
BfuI GTATCC 1 cut(s) 117
BisI GCNGC 2 cut(s) 166, 362
BlsI GCNGC 2 cut(s) 167, 363
Bme1390I CCNGG 1 cut(s) 208
Bme18I GGWCC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 428
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 1 cut(s) 109
BoxI GACNNNNGTC 2 cut(s) 33, 156
BpiI GAAGAC 1 cut(s) 359
BpmI CTGGAG 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 208
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 207, 434
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 314
Bse118I RCCGGY 2 cut(s) 218, 409
Bse1I ACTGG 2 cut(s) 115, 392
BseDI CCNNGG 2 cut(s) 207, 434
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 2 cut(s) 94, 314
BseNI ACTGG 2 cut(s) 115, 392
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 1 cut(s) 177
Bsh1236I CGCG 2 cut(s) 92, 182
BshFI GGCC 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 95, 207, 219, 410
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 94, 314
BsmAI GTCTC 3 cut(s) 76, 335, 340
BsmBI CGTCTC 2 cut(s) 76, 335
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 3 cut(s) 246, 274, 421
BspACI CCGC 9 cut(s) 6, 60, 85, 222, 258, 318, 328, 362, 379
BspANI GGCC 1 cut(s) 206
BspFNI CGCG 2 cut(s) 92, 182
BspLI GGNNCC 1 cut(s) 428
BspPI GGATC 2 cut(s) 282, 429
BspQI GCTCTTC 1 cut(s) 318
BsrBI CCGCTC 3 cut(s) 258, 328, 379
BsrFI RCCGGY 2 cut(s) 218, 409
BsrI ACTGG 2 cut(s) 115, 392
BssAI RCCGGY 2 cut(s) 218, 409
BssECI CCNNGG 2 cut(s) 207, 434
BssMI GATC 3 cut(s) 246, 274, 421
BssNI GRCGYC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 263
Bst4CI ACNGT 2 cut(s) 158, 358
Bst6I CTCTTC 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 135
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 3 cut(s) 220, 256, 260
BstDSI CCRYGG 1 cut(s) 434
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 2 cut(s) 92, 182
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 4 cut(s) 94, 182, 218, 235
BstKTI GATC 3 cut(s) 249, 277, 424
BstMAI GTCTC 3 cut(s) 76, 335, 340
BstMBI GATC 3 cut(s) 246, 274, 421
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstPAI GACNNNNGTC 2 cut(s) 33, 156
BstSCI CCNGG 1 cut(s) 206
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 2 cut(s) 92, 182
BstV1I GCAGC 1 cut(s) 177
BstV2I GAAGAC 1 cut(s) 359
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuI GTATCC 1 cut(s) 117
BsuRI GGCC 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 434
BtsCI GGATG 1 cut(s) 396
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 200, 363
Cac8I GCNNGC 3 cut(s) 220, 256, 260
CfoI GCGC 4 cut(s) 94, 182, 218, 235
Cfr10I RCCGGY 2 cut(s) 218, 409
Cfr13I GGNCC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 112, 118
CviJI RGCY 4 cut(s) 55, 168, 206, 307
CviKI_1 RGCY 4 cut(s) 55, 168, 206, 307
CviQI GTAC 2 cut(s) 112, 118
DpnI GATC 3 cut(s) 248, 276, 423
DpnII GATC 3 cut(s) 246, 274, 421
DraIII CACNNNGTG 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
EciI GGCGGA 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 371
Eco72I CACGTG 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 188
Esp3I CGTCTC 2 cut(s) 76, 335
FaiI YATR 1 cut(s) 230
FaqI GGGAC 1 cut(s) 224
FauI CCCGC 3 cut(s) 67, 215, 251
Fnu4HI GCNGC 2 cut(s) 166, 362
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 166, 362
GlaI GCGC 4 cut(s) 93, 181, 217, 234
GluI GCNGC 2 cut(s) 166, 362
GsuI CTGGAG 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 1 cut(s) 206
HapII CCGG 4 cut(s) 95, 207, 219, 410
HhaI GCGC 4 cut(s) 94, 182, 218, 235
Hin1I GRCGYC 1 cut(s) 135
Hin6I GCGC 4 cut(s) 92, 180, 216, 233
HinP1I GCGC 4 cut(s) 92, 180, 216, 233
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 4 cut(s) 129, 184, 301, 335
HpaII CCGG 4 cut(s) 95, 207, 219, 410
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 2 cut(s) 38, 370
Hpy188I TCNGA 5 cut(s) 251, 279, 290, 340, 421
Hpy188III TCNNGA 2 cut(s) 272, 425
Hpy8I GTNNAC 2 cut(s) 38, 370
Hpy99I CGWCG 6 cut(s) 29, 68, 71, 125, 128, 137
HpyAV CCTTC 2 cut(s) 85, 391
HpyCH4III ACNGT 2 cut(s) 158, 358
HpyCH4IV ACGT 3 cut(s) 135, 240, 372
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpySE526I ACGT 3 cut(s) 135, 240, 372
Hsp92I GRCGYC 1 cut(s) 135
HspAI GCGC 4 cut(s) 92, 180, 216, 233
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Kzo9I GATC 3 cut(s) 246, 274, 421
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 2 cut(s) 60, 97
LpnPI CCDG 9 cut(s) 65, 108, 128, 188, 220, 232, 350, 373, 423
Lsp1109I GCAGC 1 cut(s) 177
LweI GCATC 1 cut(s) 109
MaeII ACGT 3 cut(s) 135, 240, 372
MalI GATC 3 cut(s) 248, 276, 423
MbiI CCGCTC 3 cut(s) 258, 328, 379
MboI GATC 3 cut(s) 246, 274, 421
MboII GAAGA 2 cut(s) 335, 364
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 4 cut(s) 53, 98, 149, 169
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 364
MspI CCGG 4 cut(s) 95, 207, 219, 410
MspR9I CCNGG 1 cut(s) 208
MvnI CGCG 2 cut(s) 92, 182
MwoI GCNNNNNNNGC 1 cut(s) 251
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 208
NdeII GATC 3 cut(s) 246, 274, 421
NgoMIV GCCGGC 1 cut(s) 218
NlaIV GGNNCC 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 318
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 132
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 129, 184, 301, 335
PkrI GCNGC 2 cut(s) 167, 363
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
Ppu21I YACGTR 1 cut(s) 241
PshAI GACNNNNGTC 2 cut(s) 33, 156
PspCI CACGTG 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 428
PspPI GGNCC 1 cut(s) 427
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 2 cut(s) 113, 119
RsaNI GTAC 2 cut(s) 112, 118
SapI GCTCTTC 1 cut(s) 318
SatI GCNGC 2 cut(s) 166, 362
Sau3AI GATC 3 cut(s) 246, 274, 421
Sau96I GGNCC 1 cut(s) 427
ScrFI CCNGG 1 cut(s) 208
SduI GDGCHC 1 cut(s) 264
SetI ASST 5 cut(s) 57, 138, 170, 243, 375
SfaNI GCATC 1 cut(s) 109
SgrAI CRCCGGYG 1 cut(s) 218
SinI GGWCC 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
SsiI CCGC 9 cut(s) 6, 60, 85, 222, 258, 318, 328, 362, 379
StyD4I CCNGG 1 cut(s) 206
TaaI ACNGT 2 cut(s) 158, 358
TaiI ACGT 3 cut(s) 138, 243, 375
TaqI TCGA 3 cut(s) 132, 150, 174
TauI GCSGC 1 cut(s) 364
TfiI GAWTC 4 cut(s) 129, 184, 301, 335
TscAI CASTG 2 cut(s) 200, 363
TseI GCWGC 1 cut(s) 165
TspDTI ATGAA 1 cut(s) 136
TspGWI ACGGA 3 cut(s) 99, 141, 398
TspRI CASTG 2 cut(s) 200, 363
VpaK11BI GGWCC 1 cut(s) 427
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.