MD00G1203100.v1.1

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000184
Physical Location & Seq
Reverse (-)
48655644 .. 48656519
876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1203100.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGTGGACCCCTCTCGCAACCTCTGGTTCCGCCTCTACATCCCTACCACCACCGACGTTGCCGCCAAACTACCACTCGTTATCTACTTCCACGGTGGTGGATTCGTGTTCTTTTCTGCCAACTCACAACCCTACAACGACCTCTGCAAACGCCTCGCCACCGAACTCCCCGCAGTAGTCACCTCAGTCAACTACCGCCATGCGCCGGAGCATCGGTATCCCTCCCAATACGAAGACGGCTTCGACGTCCTCAAATTCATTGACACAACAAGAATTGAAGGCCTTGATCTTAACAAGGTTGACATCACCAGGTGTTTCCTCGCCGGAGACAGCGCTGGCGGCAACCTGGCCCACCACGTGGCAATCAAGGCGAGTAACCATGAGTTTTTGCCAGATGAGGAAAGGACCGAATCGGAAACAAGGCTTGCAAAAAAGGGCTTGGCGATTACTTTGGAGCAGACTGACTGGTATTGGAAGGCGTTTTTGCCGGAGGGGTCGGATAGGGACCACGCGGTGGCGAACGTGTTCGGACCAGAATCACGCTGTGTCTTGGGGGTGAAGTTTCCGGCGACGATTGTTTTCGTGGGAGGTTTTGATCCGTTAAAGGACTGGCAAAAGATATACTACCAGGGGTTGATGAAGAACAGGATACAAGCTTACTTGATCGAGTACCCGAATTCATTTCATGGATTTTACGCGTTTCCTGAGCTCAAAGAGTCATCTTTTTTTATTAAGGAGGTGAAGGATTTCATTGACAAGCAATCTACTTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

259

Amino Acids

29.49

Weight (kDa)

5.91

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 12 - 133 8.8e-14 BD-FAE
Abhydrolase_3 PF07859 27 - 232 3.3e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
AccB7I CCANNNNNTGG 2 cut(s) 358, 514
AccII CGCG 2 cut(s) 512, 698
AciI CCGC 6 cut(s) 31, 63, 171, 196, 339, 512
AclWI GGATC 1 cut(s) 590
AcsI RAATTY 2 cut(s) 254, 676
AcvI CACGTG 1 cut(s) 358
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 4 cut(s) 312, 358, 514, 545
AfaI GTAC 1 cut(s) 671
AfeI AGCGCT 1 cut(s) 334
AfiI CCNNNNNNNGG 3 cut(s) 205, 358, 514
AflIII ACRYGT 2 cut(s) 522, 696
AgsI TTSAA 1 cut(s) 278
AjnI CCWGG 3 cut(s) 308, 345, 627
AleI CACNNNNGTG 1 cut(s) 96
AluBI AGCT 2 cut(s) 656, 709
AluI AGCT 2 cut(s) 656, 709
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 590
Aor51HI AGCGCT 1 cut(s) 334
AoxI GGCC 2 cut(s) 280, 348
ApoI RAATTY 2 cut(s) 254, 676
Asp700I GAANNNNTTC 2 cut(s) 524, 746
AspLEI GCGC 2 cut(s) 205, 335
AspS9I GGNCC 5 cut(s) 7, 349, 405, 505, 530
AsuHPI GGTGA 4 cut(s) 172, 298, 568, 751
AvaII GGWCC 4 cut(s) 7, 405, 505, 530
BanII GRGCYC 1 cut(s) 711
BbrPI CACGTG 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 711
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 3 cut(s) 310, 347, 629
BciVI GTATCC 2 cut(s) 228, 642
BcoDI GTCTC 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 336
BfuI GTATCC 2 cut(s) 228, 642
BisI GCNGC 2 cut(s) 63, 340
BlsI GCNGC 2 cut(s) 64, 341
Bme1390I CCNGG 3 cut(s) 310, 347, 629
Bme18I GGWCC 4 cut(s) 7, 405, 505, 530
BmgT120I GGNCC 5 cut(s) 7, 349, 405, 505, 530
BmiI GGNNCC 3 cut(s) 9, 29, 506
BmrFI CCNGG 3 cut(s) 310, 347, 629
BmsI GCATC 1 cut(s) 220
BpiI GAAGAC 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 358
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 91, 628
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 358, 514
Bse1I ACTGG 2 cut(s) 470, 614
BseBI CCWGG 3 cut(s) 310, 347, 629
BseDI CCNNGG 2 cut(s) 91, 628
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 205, 358, 514
BseMII CTCAG 2 cut(s) 198, 696
BseNI ACTGG 2 cut(s) 470, 614
Bsh1236I CGCG 2 cut(s) 512, 698
BshFI GGCC 2 cut(s) 282, 350
BsiHKAI GWGCWC 1 cut(s) 711
BsiSI CCGG 4 cut(s) 206, 324, 488, 566
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 3 cut(s) 205, 358, 514
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 2 cut(s) 282, 350
Bsp1286I GDGCHC 1 cut(s) 711
Bsp143I GATC 3 cut(s) 286, 595, 663
BspACI CCGC 6 cut(s) 31, 63, 171, 196, 339, 512
BspANI GGCC 2 cut(s) 282, 350
BspCNI CTCAG 2 cut(s) 197, 697
BspFNI CGCG 2 cut(s) 512, 698
BspLI GGNNCC 3 cut(s) 9, 29, 506
BspPI GGATC 1 cut(s) 590
BsrI ACTGG 2 cut(s) 470, 614
BssECI CCNNGG 2 cut(s) 91, 628
BssMI GATC 3 cut(s) 286, 595, 663
BssNI GRCGYC 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 310, 347, 629
Bst4CI ACNGT 1 cut(s) 95
BstACI GRCGYC 1 cut(s) 246
BstBAI YACGTR 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 337, 426
BstDEI CTNAG 2 cut(s) 184, 705
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 39
BstFNI CGCG 2 cut(s) 512, 698
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 205, 335
BstKTI GATC 3 cut(s) 289, 598, 666
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 3 cut(s) 286, 595, 663
BstMWI GCNNNNNNNGC 2 cut(s) 339, 368
BstNI CCWGG 3 cut(s) 310, 347, 629
BstSCI CCNGG 3 cut(s) 308, 345, 627
BstUI CGCG 2 cut(s) 512, 698
BstV2I GAAGAC 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 98
BsuI GTATCC 2 cut(s) 228, 642
BsuRI GGCC 2 cut(s) 282, 350
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 337, 426
CfoI GCGC 2 cut(s) 205, 335
Cfr13I GGNCC 5 cut(s) 7, 349, 405, 505, 530
CsiI ACCWGGT 1 cut(s) 308
Csp6I GTAC 1 cut(s) 670
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
CviAII CATG 3 cut(s) 200, 380, 686
CviJI RGCY 7 cut(s) 240, 282, 350, 424, 438, 656, 709
CviKI_1 RGCY 7 cut(s) 240, 282, 350, 424, 438, 656, 709
CviQI GTAC 1 cut(s) 670
DdeI CTNAG 2 cut(s) 184, 705
DpnI GATC 3 cut(s) 288, 597, 665
DpnII GATC 3 cut(s) 286, 595, 663
DraIII CACNNNGTG 4 cut(s) 312, 358, 514, 545
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 709
Eco147I AGGCCT 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 711
Eco47I GGWCC 4 cut(s) 7, 405, 505, 530
Eco47III AGCGCT 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 709
Eco72I CACGTG 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 709
EcoRI GAATTC 1 cut(s) 676
EcoRII CCWGG 3 cut(s) 308, 345, 627
EcoT38I GRGCYC 1 cut(s) 711
FaeI CATG 3 cut(s) 203, 383, 689
FaiI YATR 5 cut(s) 201, 381, 622, 687, 771
FaqI GGGAC 1 cut(s) 518
FatI CATG 3 cut(s) 199, 379, 685
FauI CCCGC 1 cut(s) 178
Fnu4HI GCNGC 2 cut(s) 63, 340
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 2 cut(s) 63, 340
GlaI GCGC 2 cut(s) 204, 334
GluI GCNGC 2 cut(s) 63, 340
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 2 cut(s) 282, 350
HapII CCGG 4 cut(s) 206, 324, 488, 566
HhaI GCGC 2 cut(s) 205, 335
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 3 cut(s) 203, 383, 689
Hin6I GCGC 2 cut(s) 203, 333
HinP1I GCGC 2 cut(s) 203, 333
HincII GTYRAC 2 cut(s) 190, 301
HindII GTYRAC 2 cut(s) 190, 301
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 4 cut(s) 102, 410, 536, 716
HpaII CCGG 4 cut(s) 206, 324, 488, 566
HphI GGTGA 4 cut(s) 172, 298, 568, 751
Hpy166II GTNNAC 3 cut(s) 7, 190, 301
Hpy188I TCNGA 3 cut(s) 415, 499, 530
Hpy188III TCNNGA 1 cut(s) 704
Hpy8I GTNNAC 3 cut(s) 7, 190, 301
Hpy99I CGWCG 3 cut(s) 59, 248, 574
HpyAV CCTTC 3 cut(s) 272, 469, 736
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4IV ACGT 4 cut(s) 57, 246, 357, 522
HpyCH4V TGCA 2 cut(s) 147, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 368
HpyF3I CTNAG 2 cut(s) 184, 705
HpySE526I ACGT 4 cut(s) 57, 246, 357, 522
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 3 cut(s) 203, 383, 689
HspAI GCGC 2 cut(s) 203, 333
Kzo9I GATC 3 cut(s) 286, 595, 663
LmnI GCTCC 2 cut(s) 208, 454
LweI GCATC 1 cut(s) 220
MabI ACCWGGT 1 cut(s) 308
MaeII ACGT 4 cut(s) 57, 246, 357, 522
MaeIII GTNAC 2 cut(s) 178, 374
MalI GATC 3 cut(s) 288, 597, 665
MboI GATC 3 cut(s) 286, 595, 663
MboII GAAGA 2 cut(s) 245, 652
MhlI GDGCHC 1 cut(s) 711
MluCI AATT 3 cut(s) 254, 273, 676
MluI ACGCGT 1 cut(s) 696
MlyI GAGTC 1 cut(s) 725
MmeI TCCRAC 1 cut(s) 477
MroXI GAANNNNTTC 2 cut(s) 524, 746
MseI TTAA 3 cut(s) 291, 602, 732
MslI CAYNNNNRTG 1 cut(s) 96
MspI CCGG 4 cut(s) 206, 324, 488, 566
MspR9I CCNGG 3 cut(s) 310, 347, 629
MvaI CCWGG 3 cut(s) 310, 347, 629
MvnI CGCG 2 cut(s) 512, 698
MwoI GCNNNNNNNGC 2 cut(s) 339, 368
NdeII GATC 3 cut(s) 286, 595, 663
NlaIII CATG 3 cut(s) 203, 383, 689
NlaIV GGNNCC 3 cut(s) 9, 29, 506
NmuCI GTSAC 1 cut(s) 178
OliI CACNNNNGTG 1 cut(s) 96
PceI AGGCCT 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PdmI GAANNNNTTC 2 cut(s) 524, 746
PfeI GAWTC 3 cut(s) 102, 410, 536
PflMI CCANNNNNTGG 2 cut(s) 358, 514
PkrI GCNGC 2 cut(s) 64, 341
PleI GAGTC 1 cut(s) 724
PmaCI CACGTG 1 cut(s) 358
PmlI CACGTG 1 cut(s) 358
PpsI GAGTC 1 cut(s) 724
Ppu21I YACGTR 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 308, 345, 627
PspCI CACGTG 1 cut(s) 358
PspGI CCWGG 3 cut(s) 308, 345, 627
PspN4I GGNNCC 3 cut(s) 9, 29, 506
PspPI GGNCC 5 cut(s) 7, 349, 405, 505, 530
RsaI GTAC 1 cut(s) 671
RsaNI GTAC 1 cut(s) 670
RseI CAYNNNNRTG 1 cut(s) 96
SacI GAGCTC 1 cut(s) 711
SaqAI TTAA 3 cut(s) 291, 602, 732
SatI GCNGC 2 cut(s) 63, 340
Sau3AI GATC 3 cut(s) 286, 595, 663
Sau96I GGNCC 5 cut(s) 7, 349, 405, 505, 530
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 3 cut(s) 310, 347, 629
SduI GDGCHC 1 cut(s) 711
SexAI ACCWGGT 1 cut(s) 308
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 4 cut(s) 7, 405, 505, 530
SmiMI CAYNNNNRTG 1 cut(s) 96
Sse9I AATT 3 cut(s) 254, 273, 676
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 6 cut(s) 31, 63, 171, 196, 339, 512
SstI GAGCTC 1 cut(s) 711
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 308, 345, 627
TaaI ACNGT 1 cut(s) 95
TaiI ACGT 4 cut(s) 60, 249, 360, 525
TaqI TCGA 2 cut(s) 243, 666
TaqII GACCGA 1 cut(s) 422
TasI AATT 3 cut(s) 254, 273, 676
TauI GCSGC 2 cut(s) 65, 342
TfiI GAWTC 3 cut(s) 102, 410, 536
Tru1I TTAA 3 cut(s) 291, 602, 732
Tru9I TTAA 3 cut(s) 291, 602, 732
TseFI GTSAC 1 cut(s) 178
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 5 cut(s) 247, 653, 669, 674, 739
TspGWI ACGGA 1 cut(s) 588
Van91I CCANNNNNTGG 2 cut(s) 358, 514
VpaK11BI GGWCC 4 cut(s) 7, 405, 505, 530
XapI RAATTY 2 cut(s) 254, 676
XmnI GAANNNNTTC 2 cut(s) 524, 746
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.