Rh5BG354600

Steryl acetyl hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000184
Physical Location & Seq
Reverse (-)
54866215 .. 54867756
1542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG354600.1

Sequence Viewer

Length: 993 bp
ATGACCCGAAACCCTCCAACATCACCGGACCTTCCATGCACGCTCCGGTTCTTCCTCTTCATCCTCACCTTCGTTCTCAATACCGCTCGCCGCTCCAACGGCACGGTAAACCGCCCTTTTCTGAAAGTCTTCGACTCCAAGGCCTCTCCTTCAAACAAACGCGACGTCAAAACCACTGATGTAATGGTTGACCCCTCCCGCAATCTATGGTTCCGCCTCTTCGTCCCCACCACCACCACAACCACCAAACTCCCTGTGATAATATACTTCCATGGTGGCGGTTTCGTCTTCTTCTCTGCCAACTCAACTCTCTTCGAAGGCATCTGCAGACGACTCGCCCTGAAGCTCCCTGCCATCGTCATCTCCGTCAACTACCGTCTCGCGCCGGAGTATCGTTACCCGTCAGCTTTCGAAGACGGATTCGACGTGCTGAAGTTTGTTGATACGGAAAACTTCGAGGGGTTTCCGAGTATCGCTGACCTCGGTAGGTGTTTCCTTGCCGGTGACAGCGCCGGCGGCAACATAGCTCACCACGTGGCTGTCATGGCGGGTGAGCACGAGTTTTTGAAGATGAAAGTGATTGGCATCATAGGGATACAACCGTTTTTTGGCGGGGAAGAAAGGACTGAATCGGAAACCAAGCTTAAAAACACGTTGATACTCTCTCTGGAGATAACTGACTGGTACTGGAAGGCGTTTTTGCCGGTCGGGGAGGACAGAGACCACCCTTTGGTTAATGTATTCGGTCCCCGATCGGATGCTGTTCTTGGGATAAACTTTCCGGCATCGATTGTTTTCGTTGCTGGGTTTGATCCATTGAAAGACTGGCAAAAGAGATACTGCGAGGGGTTGAAGAAAGCTGGAAAAGAAGTTTACATGGTCGAGTACCCGAATGCATTTCATGGATTTTACTGTTTTCCTCAGCTAAAGGAGTGGTCGTTATTAGTTGAGGAGGTGAAGGATTTCATTGAGAAGCAATCAGCTGCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

330

Amino Acids

37.3

Weight (kDa)

7.01

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Say1_Mug180 PF10340 49 - 185 2.3e-06 Steryl acetyl hydrolase
COesterase PF00135 71 - 128 3.3e-07 Carboxylesterase family
BD-FAE PF20434 75 - 188 6.7e-11 BD-FAE
Abhydrolase_3 PF07859 87 - 304 2.6e-60 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AccB7I CCANNNNNTGG 1 cut(s) 730
AccBSI CCGCTC 2 cut(s) 86, 93
AccII CGCG 2 cut(s) 162, 383
AciI CCGC 9 cut(s) 84, 91, 112, 199, 214, 279, 516, 548, 612
AclWI GGATC 1 cut(s) 806
AcuI CTGAAG 2 cut(s) 362, 452
AcvI CACGTG 1 cut(s) 535
AcyI GRCGYC 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 2 cut(s) 686, 887
AfiI CCNNNNNNNGG 2 cut(s) 608, 730
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 4 cut(s) 153, 568, 820, 853
AjiI CACGTC 1 cut(s) 427
AluBI AGCT 7 cut(s) 346, 407, 527, 643, 860, 925, 983
AluI AGCT 7 cut(s) 346, 407, 527, 643, 860, 925, 983
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 2 cut(s) 383, 714
AlwI GGATC 1 cut(s) 806
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 983
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
Asp700I GAANNNNTTC 2 cut(s) 128, 962
AspLEI GCGC 2 cut(s) 385, 512
AspS9I GGNCC 2 cut(s) 28, 746
AsuHPI GGTGA 6 cut(s) 15, 58, 515, 521, 563, 967
AsuII TTCGAA 2 cut(s) 315, 411
AvaII GGWCC 2 cut(s) 28, 746
BaeI ACNNNNGTAYC 2 cut(s) 587, 620
BauI CACGAG 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 535
BbsI GAAGAC 3 cut(s) 121, 280, 420
Bbv12I GWGCWC 1 cut(s) 558
BbvCI CCTCAGC 1 cut(s) 921
BbvI GCAGC 1 cut(s) 970
BccI CCATC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciVI GTATCC 1 cut(s) 588
BcoDI GTCTC 2 cut(s) 383, 714
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 513
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 3 cut(s) 91, 517, 984
BlsI GCNGC 3 cut(s) 92, 518, 985
Bme18I GGWCC 2 cut(s) 28, 746
BmgBI CACGTC 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 28, 746
BmiI GGNNCC 2 cut(s) 212, 748
BmsI GCATC 4 cut(s) 330, 594, 748, 794
BpiI GAAGAC 3 cut(s) 121, 280, 420
BpmI CTGGAG 1 cut(s) 689
Bpu10I CCTNAGC 1 cut(s) 921
Bpu14I TTCGAA 2 cut(s) 315, 411
Bsa29I ATCGAT 1 cut(s) 788
BsaAI YACGTR 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 584
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 3 cut(s) 138, 271, 481
BsaWI WCCGGW 2 cut(s) 25, 45
BsaXI ACNNNNNCTCC 2 cut(s) 923, 953
Bsc4I CCNNNNNNNGG 2 cut(s) 608, 730
Bse118I RCCGGY 3 cut(s) 500, 512, 703
Bse1I ACTGG 3 cut(s) 686, 692, 830
Bse8I GATNNNNATC 1 cut(s) 584
BseCI ATCGAT 1 cut(s) 788
BseDI CCNNGG 3 cut(s) 138, 271, 481
BseGI GGATG 2 cut(s) 60, 763
BseJI GATNNNNATC 1 cut(s) 584
BseLI CCNNNNNNNGG 2 cut(s) 608, 730
BseMII CTCAG 1 cut(s) 935
BseNI ACTGG 3 cut(s) 686, 692, 830
BseRI GAGGAG 1 cut(s) 965
BseXI GCAGC 1 cut(s) 970
BseYI CCCAGC 1 cut(s) 803
Bsh1236I CGCG 2 cut(s) 162, 383
Bsh1285I CGRYCG 2 cut(s) 708, 755
BshFI GGCC 1 cut(s) 143
BshVI ATCGAT 1 cut(s) 788
BsiEI CGRYCG 2 cut(s) 708, 755
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 7 cut(s) 26, 46, 386, 501, 513, 704, 782
BslFI GGGAC 2 cut(s) 209, 732
BslI CCNNNNNNNGG 2 cut(s) 608, 730
BsmAI GTCTC 2 cut(s) 383, 714
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 209, 732
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 714
Bsp119I TTCGAA 2 cut(s) 315, 411
Bsp1286I GDGCHC 1 cut(s) 558
Bsp143I GATC 2 cut(s) 752, 811
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 9 cut(s) 84, 91, 112, 199, 214, 279, 516, 548, 612
BspANI GGCC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 934
BspDI ATCGAT 1 cut(s) 788
BspFNI CGCG 2 cut(s) 162, 383
BspLI GGNNCC 2 cut(s) 212, 748
BspMAI CTGCAG 1 cut(s) 329
BspPI GGATC 1 cut(s) 806
BspT104I TTCGAA 2 cut(s) 315, 411
BspTNI GGTCTC 1 cut(s) 714
BsrBI CCGCTC 2 cut(s) 86, 93
BsrFI RCCGGY 3 cut(s) 500, 512, 703
BsrI ACTGG 3 cut(s) 686, 692, 830
BssAI RCCGGY 3 cut(s) 500, 512, 703
BssECI CCNNGG 3 cut(s) 138, 271, 481
BssMI GATC 2 cut(s) 752, 811
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 1 cut(s) 557
BssT1I CCWWGG 2 cut(s) 138, 271
Bst2BI CACGAG 1 cut(s) 557
Bst4CI ACNGT 4 cut(s) 106, 377, 603, 914
Bst6I CTCTTC 3 cut(s) 62, 224, 317
BstACI GRCGYC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 535
BstBI TTCGAA 2 cut(s) 315, 411
BstC8I GCNNGC 3 cut(s) 41, 88, 514
BstDEI CTNAG 2 cut(s) 921, 987
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 2 cut(s) 60, 763
BstFNI CGCG 2 cut(s) 162, 383
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 2 cut(s) 385, 512
BstKTI GATC 2 cut(s) 755, 814
BstMAI GTCTC 2 cut(s) 383, 714
BstMBI GATC 2 cut(s) 752, 811
BstMCI CGRYCG 2 cut(s) 708, 755
BstMWI GCNNNNNNNGC 3 cut(s) 99, 516, 545
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 2 cut(s) 162, 383
BstV1I GCAGC 1 cut(s) 970
BstV2I GAAGAC 3 cut(s) 121, 280, 420
Bsu15I ATCGAT 1 cut(s) 788
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 1 cut(s) 143
BsuTUI ATCGAT 1 cut(s) 788
BtgI CCRYGG 1 cut(s) 271
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 2 cut(s) 60, 763
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 3 cut(s) 41, 88, 514
CfoI GCGC 2 cut(s) 385, 512
Cfr10I RCCGGY 3 cut(s) 500, 512, 703
Cfr13I GGNCC 2 cut(s) 28, 746
ClaI ATCGAT 1 cut(s) 788
Csp6I GTAC 2 cut(s) 685, 886
CviAII CATG 5 cut(s) 36, 272, 544, 877, 902
CviJI RGCY 9 cut(s) 143, 346, 407, 527, 539, 643, 860, 925, 983
CviKI_1 RGCY 9 cut(s) 143, 346, 407, 527, 539, 643, 860, 925, 983
CviQI GTAC 2 cut(s) 685, 886
DdeI CTNAG 2 cut(s) 921, 987
DpnI GATC 2 cut(s) 754, 813
DpnII GATC 2 cut(s) 752, 811
DraIII CACNNNGTG 1 cut(s) 535
Eam1104I CTCTTC 3 cut(s) 62, 224, 317
EarI CTCTTC 3 cut(s) 62, 224, 317
EciI GGCGGA 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 138, 271
Eco147I AGGCCT 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 714
Eco47I GGWCC 2 cut(s) 28, 746
Eco57I CTGAAG 2 cut(s) 362, 452
Eco72I CACGTG 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 138, 271
EcoT22I ATGCAT 1 cut(s) 898
ErhI CCWWGG 2 cut(s) 138, 271
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 5 cut(s) 39, 275, 547, 880, 905
FaiI YATR 9 cut(s) 37, 208, 265, 273, 524, 545, 590, 878, 903
FaqI GGGAC 2 cut(s) 209, 732
FatI CATG 5 cut(s) 35, 271, 543, 876, 901
FauI CCCGC 3 cut(s) 206, 541, 605
Fnu4HI GCNGC 3 cut(s) 91, 517, 984
FokI GGATG 2 cut(s) 47, 770
Fsp4HI GCNGC 3 cut(s) 91, 517, 984
GlaI GCGC 2 cut(s) 384, 511
GluI GCNGC 3 cut(s) 91, 517, 984
GsaI CCCAGC 1 cut(s) 807
GsuI CTGGAG 1 cut(s) 689
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 1 cut(s) 143
HapII CCGG 7 cut(s) 26, 46, 386, 501, 513, 704, 782
HhaI GCGC 2 cut(s) 385, 512
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 5 cut(s) 39, 275, 547, 880, 905
Hin6I GCGC 2 cut(s) 383, 510
HinP1I GCGC 2 cut(s) 383, 510
HincII GTYRAC 2 cut(s) 190, 370
HindII GTYRAC 2 cut(s) 190, 370
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 4 cut(s) 134, 333, 420, 629
HpaII CCGG 7 cut(s) 26, 46, 386, 501, 513, 704, 782
HphI GGTGA 6 cut(s) 15, 58, 515, 521, 563, 967
Hpy166II GTNNAC 4 cut(s) 109, 190, 370, 874
Hpy188I TCNGA 4 cut(s) 123, 468, 634, 757
Hpy188III TCNNGA 1 cut(s) 668
Hpy8I GTNNAC 4 cut(s) 109, 190, 370, 874
Hpy99I CGWCG 2 cut(s) 167, 428
HpyAV CCTTC 6 cut(s) 41, 79, 159, 311, 685, 952
HpyCH4III ACNGT 4 cut(s) 106, 377, 603, 914
HpyCH4IV ACGT 4 cut(s) 165, 426, 534, 653
HpyCH4V TGCA 3 cut(s) 39, 327, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 516, 545
HpyF3I CTNAG 2 cut(s) 921, 987
HpySE526I ACGT 4 cut(s) 165, 426, 534, 653
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 5 cut(s) 39, 275, 547, 880, 905
HspAI GCGC 2 cut(s) 383, 510
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Kzo9I GATC 2 cut(s) 752, 811
LmnI GCTCC 3 cut(s) 48, 98, 351
Lsp1109I GCAGC 1 cut(s) 970
LweI GCATC 4 cut(s) 330, 594, 748, 794
MaeII ACGT 4 cut(s) 165, 426, 534, 653
MaeIII GTNAC 2 cut(s) 395, 503
MalI GATC 2 cut(s) 754, 813
MbiI CCGCTC 2 cut(s) 86, 93
MboI GATC 2 cut(s) 752, 811
MhlI GDGCHC 1 cut(s) 558
MlyI GAGTC 2 cut(s) 128, 327
MmeI TCCRAC 2 cut(s) 41, 120
Mph1103I ATGCAT 1 cut(s) 898
MreI CGCCGGCG 1 cut(s) 512
MroNI GCCGGC 1 cut(s) 512
MroXI GAANNNNTTC 2 cut(s) 128, 962
MseI TTAA 2 cut(s) 645, 735
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 7 cut(s) 26, 46, 386, 501, 513, 704, 782
Mva1269I GAATGC 1 cut(s) 898
MvnI CGCG 2 cut(s) 162, 383
MwoI GCNNNNNNNGC 3 cut(s) 99, 516, 545
NaeI GCCGGC 1 cut(s) 514
NcoI CCATGG 1 cut(s) 271
NdeII GATC 2 cut(s) 752, 811
NgoMIV GCCGGC 1 cut(s) 512
NlaIII CATG 5 cut(s) 39, 275, 547, 880, 905
NlaIV GGNNCC 2 cut(s) 212, 748
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 898
NspV TTCGAA 2 cut(s) 315, 411
PceI AGGCCT 1 cut(s) 143
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 423
PctI GAATGC 1 cut(s) 898
PdiI GCCGGC 1 cut(s) 514
PdmI GAANNNNTTC 2 cut(s) 128, 962
PfeI GAWTC 2 cut(s) 420, 629
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 3 cut(s) 92, 518, 985
Ple19I CGATCG 1 cut(s) 755
PleI GAGTC 2 cut(s) 128, 327
PmaCI CACGTG 1 cut(s) 535
PmlI CACGTG 1 cut(s) 535
PpsI GAGTC 2 cut(s) 128, 327
Ppu21I YACGTR 1 cut(s) 535
PspCI CACGTG 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 803
PspN4I GGNNCC 2 cut(s) 212, 748
PspPI GGNCC 2 cut(s) 28, 746
PstI CTGCAG 1 cut(s) 329
PvuI CGATCG 1 cut(s) 755
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 2 cut(s) 686, 887
RsaNI GTAC 2 cut(s) 685, 886
SaqAI TTAA 2 cut(s) 645, 735
SatI GCNGC 3 cut(s) 91, 517, 984
Sau3AI GATC 2 cut(s) 752, 811
Sau96I GGNCC 2 cut(s) 28, 746
SchI GAGTC 2 cut(s) 128, 327
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 4 cut(s) 330, 594, 748, 794
SfcI CTRYAG 1 cut(s) 325
SfuI TTCGAA 2 cut(s) 315, 411
SgrAI CRCCGGYG 1 cut(s) 512
SinI GGWCC 2 cut(s) 28, 746
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 9 cut(s) 84, 91, 112, 199, 214, 279, 516, 548, 612
StuI AGGCCT 1 cut(s) 143
StyI CCWWGG 2 cut(s) 138, 271
TaaI ACNGT 4 cut(s) 106, 377, 603, 914
TaiI ACGT 4 cut(s) 168, 429, 537, 656
TaqI TCGA 7 cut(s) 132, 315, 411, 423, 456, 788, 882
TaqII GACCGA 1 cut(s) 734
TauI GCSGC 2 cut(s) 93, 519
TfiI GAWTC 2 cut(s) 420, 629
Tru1I TTAA 2 cut(s) 645, 735
Tru9I TTAA 2 cut(s) 645, 735
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 1 cut(s) 983
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 4 cut(s) 49, 587, 890, 955
TspGWI ACGGA 3 cut(s) 355, 432, 461
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 28, 746
XcmI CCANNNNNNNNNTGG 2 cut(s) 181, 822
XmnI GAANNNNTTC 2 cut(s) 128, 962
ZraI GACGTC 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.