RLG00000022548

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000184
Physical Location & Seq
Reverse (-)
3383573 .. 3384365
793 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022548

Sequence Viewer

Length: 669 bp
ATGACGACGTCGTCTCAGTCCCAACAATCCACTCCCTCCTCCTGGGCTTTGCTCCCTTGGAGGACCCGGATCTCGTTATCGCTTCTGTCAAAGTTAACGGACGCCGCTCGCCGACCAAACGGCACCATCAACCGCCGTCTCATGAGCTTCCTCGACTTACGCTCCTCCGCCACTCCCTCAGCTCCCTACAAAGGCGTCACCACCTCCGACGTCACCGTCGACCAAACCTGCAACCTCTGGTTCCGCTTCTTCGTCCCCTCTAAGACGTCGTCTTCCCCCGCCTCTTTACCCGTCGTCGTTTTCTTCCACGGCGGCGGCTTCAGCTTTCTCAGCCCTGACTCCTACGCCTACGACGCCGTCTGCCGCAAATTCGCCCTCGAAATCCCCGCCGTCGTAGTCTCCATCAACTACCGCCTCTCCCCGGAGCACGGCTACCCCTGCCAGTACGATGACGGCCTCGACGTCCTCAAATTCCTCGACCAAAACCCCGACTCCCTCCCCGACCTCGCTGACTGCTCCCGCTGCTTCCTCGCCGGAGACAGCGCCGGCGTGAACATTGCCCACCATGTGGCGGTGCGAGCTTGCCGCGAGCCGTTTCGGGTCGTGAAACCAATCGGGTTGATATCGATCCAACCGTTCTTCGGCGGCGAGGAGACTGAGGAGATTTGA

Protein Analysis

223

Amino Acids

24.4

Weight (kDa)

6.41

Isoelectric Point (pI)

60.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 83 - 141 7.4e-09 Carboxylesterase family
Say1_Mug180 PF10340 86 - 214 4.8e-06 Steryl acetyl hydrolase
BD-FAE PF20434 93 - 193 4.8e-11 BD-FAE
Abhydrolase_3 PF07859 99 - 214 1.7e-32 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23530
fragaria_vesca FvH4_2g00440 FvH4_2g09720 FvH4_3g28580 FvH4_3g28600
malus_domestica MD00G1203100.v1.1 MD03G1173000.v1.1 MD03G1173100.v1.1 MD05G1060700.v1.1 MD10G1068500.v1.1 MD11G1191000.v1.1
prunus_persica Prupe.4G252600_v2.0.a1 Prupe.8G004100_v2.0.a1 Prupe.8G090400_v2.0.a1
pyrus_communis pycom03g13020 pycom05g05160 pycom10g05450
rosa_chinensis RchiOBHm_Chr2g0154081 RchiOBHm_Chr3g0458401 RchiOBHm_Chr4g0422631 RchiOBHm_Chr5g0013851 RchiOBHm_Chr5g0052531 RchiOBHm_Chr5g0052561 RchiOBHm_Chr5g0062251 RchiOBHm_Chr6g0249211 RchiOBHm_Chr6g0249811 RchiOBHm_Chr6g0250041 RchiOBHm_Chr6g0250061 RchiOBHm_Chr6g0250071 RchiOBHm_Chr6g0250101 RchiOBHm_Chr6g0250261 RchiOBHm_Chr6g0250271 RchiOBHm_Chr6g0250281 RchiOBHm_Chr6g0250341 RchiOBHm_Chr6g0250361 RchiOBHm_Chr6g0266021 RchiOBHm_Chr7g0181171 RchiOBHm_Chr7g0181191
rosa_laevigata RLG00000002200 RLG00000007738 RLG00000014126 RLG00000014137 RLG00000015547 RLG00000015553 RLG00000019990 RLG00000022548 RLG00000025161 RLG00000034812 RLG00000034813 RLG00000035546
rosa_multiflora Rmu_co8002032.1_g000001 Rmu_co8108364.1_g000001 Rmu_co8201470.1_g000001 Rmu_co8274775.1_g000001 Rmu_sc0000441.1_g000004 Rmu_sc0000704.1_g000006 Rmu_sc0000704.1_g000038 Rmu_sc0000704.1_g000044 Rmu_sc0000776.1_g000016 Rmu_sc0000830.1_g000025 Rmu_sc0001147.1_g000015 Rmu_sc0001147.1_g000016 Rmu_sc0001209.1_g000044 Rmu_sc0002200.1_g000049 Rmu_sc0002311.1_g000008 Rmu_sc0003627.1_g000015 Rmu_sc0004691.1_g000004 Rmu_sc0005070.1_g000006 Rmu_sc0005798.1_g000012 Rmu_sc0006032.1_g000002 Rmu_sc0006032.1_g000009 Rmu_sc0006110.1_g000003 Rmu_sc0006803.1_g000002 Rmu_sc0007793.1_g000026 Rmu_sc0008209.1_g000004 Rmu_sc0008209.1_g000007 Rmu_sc0009002.1_g000003 Rmu_sc0009002.1_g000008 Rmu_sc0013424.1_g000007 Rmu_sc0013809.1_g000001 Rmu_sc0015580.1_g000005 Rmu_sc0015938.1_g000005 Rmu_sc0022309.1_g000001 Rmu_ssc0000066.1_g000013 Rmu_ssc0000409.1_g000028
rosa_roxburghii Rroxscaffold_174G00435120 Rroxscaffold_1G00028400 Rroxscaffold_1G00028440 Rroxscaffold_1G00028450 Rroxscaffold_6G00421100 Rroxscaffold_7G00202080 Rroxscaffold_7G00216660 Rroxscaffold_7G00217380
rosa_rugosa Rorug03G0027600 Rorug05G0270900 Rorug05G0271000 Rorug05G0347300 Rorug05G0494400 Rorug05G0494500 Rorug05G0495500 Rorug06G0022600 Rorug06G0324400 Rorug06G0439500
rosa_samantha Rh1CG424500 Rh2AG352300 Rh2BG391600 Rh3AG087400 Rh3CG090500 Rh3DG091100 Rh3DG119700 Rh4CG247600 Rh4DG207900 Rh5AG408000 Rh5BG354600 Rh5BG354800 Rh5BG421800 Rh5BG423400 Rh5CG115500 Rh5CG380400 Rh5CG380500 Rh5CG445800 Rh5CG447200 Rh5DG370100 Rh5DG370300 Rh5DG436700 Rh5DG438000 Rh5DG534300 Rh6AG004500 Rh6AG005000 Rh6AG005100 Rh6AG005400 Rh6AG005500 Rh6AG085300 Rh6AG143900 Rh6BG005100 Rh6BG005200 Rh6BG005300 Rh6BG144200 Rh6CG003500 Rh6CG074600 Rh6CG140900 Rh6DG003300 Rh6DG071500 Rh6DG128600 Rh7BG040400 Rh7BG040600 Rh7CG026000 Rh7CG042800 Rh7CG433400 Rh7DG040800 Rh7DG040900
rosa_wichuraiana Rw0G012560 Rw1G010810 Rw3G007420 Rw5G032480 Rw5G032500 Rw5G038450 Rw5G038560 Rw6G000490 Rw6G012430 Rw7G003390 Rw7G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 10, 215
AatII GACGTC 4 cut(s) 11, 213, 269, 465
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 588
AclWI GGATC 2 cut(s) 77, 622
AcsI RAATTY 2 cut(s) 368, 470
AcuI CTGAAG 1 cut(s) 304
AcyI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
AdeI CACNNNGTG 1 cut(s) 568
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 7 cut(s) 42, 191, 421, 428, 568, 571, 641
AjnI CCWGG 1 cut(s) 41
AluBI AGCT 4 cut(s) 147, 182, 324, 581
AluI AGCT 4 cut(s) 147, 182, 324, 581
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 5 cut(s) 18, 143, 403, 531, 647
AlwI GGATC 2 cut(s) 77, 622
AoxI GGCC 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 2 cut(s) 368, 470
AspLEI GCGC 1 cut(s) 545
AspS9I GGNCC 1 cut(s) 63
AsuC2I CCSGG 2 cut(s) 67, 422
AsuHPI GGTGA 2 cut(s) 190, 205
AvaII GGWCC 1 cut(s) 63
BanI GGYRCC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 429
BbvCI CCTCAGC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 509
BccI CCATC 2 cut(s) 134, 410
BceAI ACGGC 8 cut(s) 120, 136, 325, 341, 374, 445, 469, 577
BciT130I CCWGG 1 cut(s) 43
BcnI CCSGG 2 cut(s) 67, 422
BcoDI GTCTC 5 cut(s) 18, 143, 403, 531, 647
BfoI RGCGCY 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 7 cut(s) 105, 313, 316, 364, 523, 586, 646
BlsI GCNGC 7 cut(s) 106, 314, 317, 365, 524, 587, 647
Bme1390I CCNGG 3 cut(s) 43, 67, 422
Bme18I GGWCC 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 3 cut(s) 65, 124, 242
BmrFI CCNGG 3 cut(s) 43, 67, 422
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 178
BpuMI CCSGG 2 cut(s) 67, 422
Bsa29I ATCGAT 1 cut(s) 626
BsaBI GATNNNNATC 1 cut(s) 626
BsaHI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
BsaJI CCNNGG 4 cut(s) 42, 56, 307, 420
BsaXI ACNNNNNCTCC 6 cut(s) 146, 176, 401, 431, 476, 506
Bsc4I CCNNNNNNNGG 7 cut(s) 42, 191, 421, 428, 568, 571, 641
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 1 cut(s) 442
Bse3DI GCAATG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 1 cut(s) 43
BseCI ATCGAT 1 cut(s) 626
BseDI CCNNGG 4 cut(s) 42, 56, 307, 420
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 7 cut(s) 42, 191, 421, 428, 568, 571, 641
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 4 cut(s) 29, 192, 343, 648
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 3 cut(s) 28, 154, 665
BseXI GCAGC 1 cut(s) 509
Bsh1236I CGCG 1 cut(s) 588
BshFI GGCC 1 cut(s) 456
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 626
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 4 cut(s) 67, 422, 534, 546
BslFI GGGAC 2 cut(s) 4, 239
BslI CCNNNNNNNGG 7 cut(s) 42, 191, 421, 428, 568, 571, 641
BsmAI GTCTC 5 cut(s) 18, 143, 403, 531, 647
BsmBI CGTCTC 2 cut(s) 18, 143
BsmFI GGGAC 2 cut(s) 4, 239
BsnI GGCC 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 2 cut(s) 69, 627
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 4 cut(s) 28, 191, 342, 649
BspDI ATCGAT 1 cut(s) 626
BspFNI CGCG 1 cut(s) 588
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 3 cut(s) 65, 124, 242
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 2 cut(s) 77, 622
BspT107I GGYRCC 1 cut(s) 122
BsrBI CCGCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 1 cut(s) 442
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 4 cut(s) 42, 56, 307, 420
BssMI GATC 2 cut(s) 69, 627
BssNI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
BssT1I CCWWGG 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 2 cut(s) 217, 636
BstACI GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
BstC8I GCNNGC 5 cut(s) 109, 547, 579, 583, 590
BstDEI CTNAG 5 cut(s) 15, 178, 261, 329, 657
BstDSI CCRYGG 1 cut(s) 307
BstFNI CGCG 1 cut(s) 588
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 2 cut(s) 72, 630
BstMAI GTCTC 5 cut(s) 18, 143, 403, 531, 647
BstMBI GATC 2 cut(s) 69, 627
BstMWI GCNNNNNNNGC 6 cut(s) 321, 330, 353, 438, 522, 578
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 3 cut(s) 41, 65, 420
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 69
BstYI RGATCY 1 cut(s) 69
Bsu15I ATCGAT 1 cut(s) 626
BsuRI GGCC 1 cut(s) 456
BsuTUI ATCGAT 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 307
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 5 cut(s) 109, 547, 579, 583, 590
CciI TCATGA 1 cut(s) 141
CfoI GCGC 1 cut(s) 545
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 63
ClaI ATCGAT 1 cut(s) 626
CseI GACGC 3 cut(s) 110, 184, 362
Csp6I GTAC 1 cut(s) 445
CviAII CATG 2 cut(s) 142, 566
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 5 cut(s) 15, 178, 261, 329, 657
DpnI GATC 2 cut(s) 71, 629
DpnII GATC 2 cut(s) 69, 627
DraIII CACNNNGTG 1 cut(s) 568
DrdI GACNNNNNNGTC 2 cut(s) 10, 215
DseDI GACNNNNNNGTC 2 cut(s) 10, 215
EciI GGCGGA 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 56
Eco32I GATATC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 63
Eco57I CTGAAG 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 41
EcoRV GATATC 1 cut(s) 624
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
Esp3I CGTCTC 2 cut(s) 18, 143
FaeI CATG 2 cut(s) 145, 569
FaiI YATR 2 cut(s) 143, 567
FaqI GGGAC 2 cut(s) 4, 239
FatI CATG 2 cut(s) 141, 565
FauI CCCGC 3 cut(s) 286, 394, 527
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 7 cut(s) 105, 313, 316, 364, 523, 586, 646
Fsp4HI GCNGC 7 cut(s) 105, 313, 316, 364, 523, 586, 646
GlaI GCGC 1 cut(s) 544
GluI GCNGC 7 cut(s) 105, 313, 316, 364, 523, 586, 646
HaeII RGCGCY 1 cut(s) 546
HaeIII GGCC 1 cut(s) 456
HapII CCGG 4 cut(s) 67, 422, 534, 546
HgaI GACGC 3 cut(s) 110, 184, 362
HhaI GCGC 1 cut(s) 545
Hin1I GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
Hin1II CATG 2 cut(s) 145, 569
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HincII GTYRAC 2 cut(s) 96, 220
HindII GTYRAC 2 cut(s) 96, 220
HinfI GANTC 2 cut(s) 338, 491
HpaI GTTAAC 1 cut(s) 96
HpaII CCGG 4 cut(s) 67, 422, 534, 546
HphI GGTGA 2 cut(s) 190, 205
Hpy166II GTNNAC 3 cut(s) 96, 220, 553
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 2 cut(s) 142, 604
Hpy8I GTNNAC 3 cut(s) 96, 220, 553
HpyCH4III ACNGT 2 cut(s) 217, 636
HpyCH4IV ACGT 4 cut(s) 8, 210, 266, 462
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 6 cut(s) 321, 330, 353, 438, 522, 578
HpyF3I CTNAG 5 cut(s) 15, 178, 261, 329, 657
HpySE526I ACGT 4 cut(s) 8, 210, 266, 462
Hsp92I GRCGYC 7 cut(s) 8, 102, 195, 210, 266, 354, 462
Hsp92II CATG 2 cut(s) 145, 569
HspAI GCGC 1 cut(s) 543
KroI GCCGGC 1 cut(s) 545
KroNI GCCGGC 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 96
Kzo9I GATC 2 cut(s) 69, 627
LmnI GCTCC 5 cut(s) 57, 167, 187, 424, 521
Lsp1109I GCAGC 1 cut(s) 509
MaeII ACGT 4 cut(s) 8, 210, 266, 462
MaeIII GTNAC 2 cut(s) 196, 211
MalI GATC 2 cut(s) 71, 629
MbiI CCGCTC 1 cut(s) 107
MboI GATC 2 cut(s) 69, 627
MboII GAAGA 4 cut(s) 241, 264, 295, 631
MflI RGATCY 1 cut(s) 69
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 2 cut(s) 368, 470
MlyI GAGTC 2 cut(s) 332, 485
MmeI TCCRAC 2 cut(s) 231, 655
MreI CGCCGGCG 1 cut(s) 545
MroNI GCCGGC 1 cut(s) 545
MseI TTAA 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 522
MspI CCGG 4 cut(s) 67, 422, 534, 546
MspR9I CCNGG 3 cut(s) 43, 67, 422
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 6 cut(s) 321, 330, 353, 438, 522, 578
NaeI GCCGGC 1 cut(s) 547
NciI CCSGG 2 cut(s) 67, 422
NdeII GATC 2 cut(s) 69, 627
NgoMIV GCCGGC 1 cut(s) 545
NlaIII CATG 2 cut(s) 145, 569
NlaIV GGNNCC 3 cut(s) 65, 124, 242
NmuCI GTSAC 2 cut(s) 196, 211
PagI TCATGA 1 cut(s) 141
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 459, 632
PdiI GCCGGC 1 cut(s) 547
PflFI GACNNNGTC 4 cut(s) 7, 10, 268, 356
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 7 cut(s) 106, 314, 317, 365, 524, 587, 647
PleI GAGTC 2 cut(s) 332, 485
PpsI GAGTC 2 cut(s) 332, 485
PpuMI RGGWCCY 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 3 cut(s) 65, 124, 242
PspPI GGNCC 1 cut(s) 63
PspPPI RGGWCCY 1 cut(s) 63
PsuI RGATCY 1 cut(s) 69
PsyI GACNNNGTC 4 cut(s) 7, 10, 268, 356
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 7 cut(s) 105, 313, 316, 364, 523, 586, 646
Sau3AI GATC 2 cut(s) 69, 627
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 2 cut(s) 332, 485
ScrFI CCNGG 3 cut(s) 43, 67, 422
SduI GDGCHC 1 cut(s) 429
SgrAI CRCCGGYG 1 cut(s) 545
SinI GGWCC 1 cut(s) 63
Sse9I AATT 2 cut(s) 368, 470
StyD4I CCNGG 3 cut(s) 41, 65, 420
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 2 cut(s) 217, 636
TaiI ACGT 4 cut(s) 11, 213, 269, 465
TaqI TCGA 6 cut(s) 153, 219, 378, 459, 477, 626
TasI AATT 2 cut(s) 368, 470
TauI GCSGC 6 cut(s) 107, 315, 318, 366, 588, 648
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TseFI GTSAC 2 cut(s) 196, 211
TseI GCWGC 1 cut(s) 522
Tsp45I GTSAC 2 cut(s) 196, 211
TspGWI ACGGA 1 cut(s) 113
Tth111I GACNNNGTC 4 cut(s) 7, 10, 268, 356
Van91I CCANNNNNTGG 1 cut(s) 568
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 2 cut(s) 368, 470
XmiI GTMKAC 1 cut(s) 219
ZraI GACGTC 4 cut(s) 9, 211, 267, 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.