FvH4_4g08647

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000863
Physical Location & Seq
Forward (+)
9110122 .. 9110694
573 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08647.t1

Sequence Viewer

Length: 573 bp
ATGGAAAAGAGGCCACCCGAGGCCAAGCCTTTTAAAGTGGACAAGTATAAGGGCACCGAAGACCCTCATCTTCACCTTGAAATATTCCTCTCGTTATGCAGTTCCACTCGGTACACAGATGCTATGGCCTGTCATGCCTTCCAAGAGACCCTCTCCGATGAAGCGCTTAGTTGGTTCCTTAACTTGCCTCCCAACTCCATTGACAATTTCGACCAGCTCTCGGAACGCTTCCTCAACCGCTTCATACTCCATGCCAGCATCTATCGCAGTGTGGATGCCTTGTTCCACATCAAGCAAAAAGAGGGTGAAGGACTCCATGAGTTACTGTCCAGGTGGCAATCAGCCACGGCACGCTGTCACAACTTGGAACCCCGGGTCGCTGAGCAAGCTTTTCGCCAATCTTTGCTTCCCAACAGCTTCCTAATGGAGATTAACAAGAACCCTCTAGGGTCATACGATGCCTTGATGGACCTTGCTGTCCGCTGGGCACGCGCCGAGTTTGCCACCTTTGGACATTCTAACCCAACTACCACATTAGCACTCCTAGCCCCACCCCCCAAGCAGCAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

191

Amino Acids

21.73

Weight (kDa)

6.23

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 47 - 135 1.3e-11 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000863)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29436 FvH4_4g08647
prunus_persica Prupe.1G244600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041081 RchiOBHm_Chr7g0227381
rosa_multiflora Rmu_co8094356.1_g000001 Rmu_co8097010.1_g000001 Rmu_co8307317.1_g000001 Rmu_co8331341.1_g000001 Rmu_co8353833.1_g000001 Rmu_co8426601.1_g000001 Rmu_co8464885.1_g000001 Rmu_sc0000161.1_g000045 Rmu_sc0000367.1_g000181 Rmu_sc0000438.1_g000020 Rmu_sc0000637.1_g000008 Rmu_sc0000706.1_g000036 Rmu_sc0000745.1_g000002 Rmu_sc0000964.1_g000007 Rmu_sc0001016.1_g000045 Rmu_sc0001190.1_g000046 Rmu_sc0002014.1_g000040 Rmu_sc0002229.1_g000012 Rmu_sc0002279.1_g000021 Rmu_sc0002279.1_g000049 Rmu_sc0002308.1_g000074 Rmu_sc0002482.1_g000017 Rmu_sc0002809.1_g000002 Rmu_sc0003147.1_g000020 Rmu_sc0003226.1_g000065 Rmu_sc0003246.1_g000004 Rmu_sc0003260.1_g000011 Rmu_sc0003441.1_g000081 Rmu_sc0003895.1_g000019 Rmu_sc0004228.1_g000001 Rmu_sc0004646.1_g000038 Rmu_sc0004688.1_g000018 Rmu_sc0005389.1_g000016 Rmu_sc0005537.1_g000011 Rmu_sc0005954.1_g000010 Rmu_sc0006021.1_g000020 Rmu_sc0006695.1_g000056 Rmu_sc0006822.1_g000010 Rmu_sc0007877.1_g000013 Rmu_sc0008049.1_g000067 Rmu_sc0008691.1_g000004 Rmu_sc0009206.1_g000002 Rmu_sc0011734.1_g000008 Rmu_sc0012136.1_g000012 Rmu_sc0012746.1_g000018 Rmu_sc0013612.1_g000011 Rmu_sc0016149.1_g000005 Rmu_sc0018867.1_g000011 Rmu_sc0020611.1_g000002 Rmu_sc0021291.1_g000004 Rmu_sc0021291.1_g000005 Rmu_sc0022400.1_g000007 Rmu_sc0025427.1_g000001 Rmu_sc0027953.1_g000002 Rmu_sc0033748.1_g000002 Rmu_ssc0000072.1_g000004 Rmu_ssc0000197.1_g000082
rosa_roxburghii Rroxscaffold_4G00282700 Rroxscaffold_5G00374470 Rroxscaffold_7G00183200
rosa_rugosa Rorug02G0187100 Rorug05G0255100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 476
AccB1I GGYRCC 1 cut(s) 53
AccII CGCG 1 cut(s) 492
AciI CCGC 2 cut(s) 238, 481
AfaI GTAC 1 cut(s) 113
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 220
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 1 cut(s) 329
AjuI GAANNNNNNNTTGG 2 cut(s) 390, 422
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 3 cut(s) 217, 389, 417
AluI AGCT 3 cut(s) 217, 389, 417
Alw26I GTCTC 1 cut(s) 140
Ama87I CYCGRG 2 cut(s) 17, 372
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 3 cut(s) 11, 21, 126
ApeKI GCWGC 1 cut(s) 562
AspLEI GCGC 2 cut(s) 166, 494
AspS9I GGNCC 1 cut(s) 469
AsuC2I CCSGG 2 cut(s) 373, 374
AsuHPI GGTGA 2 cut(s) 65, 317
AvaI CYCGRG 2 cut(s) 17, 372
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 2 cut(s) 56, 490
BanI GGYRCC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 66
BccI CCATC 1 cut(s) 460
BceAI ACGGC 1 cut(s) 363
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 2 cut(s) 373, 374
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 3 cut(s) 446, 545, 571
BfoI RGCGCY 1 cut(s) 167
BisI GCNGC 1 cut(s) 563
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 564
Bme1390I CCNGG 3 cut(s) 331, 373, 374
Bme18I GGWCC 1 cut(s) 469
BmeT110I CYCGRG 2 cut(s) 17, 372
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 3 cut(s) 55, 176, 369
BmrFI CCNGG 3 cut(s) 331, 373, 374
BmsI GCATC 4 cut(s) 109, 265, 267, 448
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 137, 169
Bpu1102I GCTNAGC 1 cut(s) 381
BpuMI CCSGG 2 cut(s) 373, 374
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 18, 345, 371, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 4 cut(s) 18, 345, 371, 372
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 372
BseSI GKGCMC 2 cut(s) 56, 490
BseYI CCCAGC 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 3 cut(s) 13, 23, 128
BshNI GGYRCC 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 17, 372
BsiSI CCGG 1 cut(s) 373
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 1 cut(s) 140
BsnI GGCC 3 cut(s) 13, 23, 128
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 2 cut(s) 17, 372
Bsp1286I GDGCHC 2 cut(s) 56, 490
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 2 cut(s) 238, 481
BspANI GGCC 3 cut(s) 13, 23, 128
BspCNI CTCAG 1 cut(s) 373
BspFNI CGCG 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 55, 176, 369
BspT107I GGYRCC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 140
BssECI CCNNGG 4 cut(s) 18, 345, 371, 372
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 1 cut(s) 327
BstC8I GCNNGC 4 cut(s) 256, 352, 387, 490
BstDEI CTNAG 2 cut(s) 167, 381
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 492
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 2 cut(s) 166, 494
BstMAI GTCTC 1 cut(s) 140
BstMWI GCNNNNNNNGC 6 cut(s) 134, 264, 386, 489, 500, 545
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 329, 371, 372
BstSLI GKGCMC 2 cut(s) 56, 490
BstUI CGCG 1 cut(s) 492
BstV2I GAAGAC 1 cut(s) 66
BsuRI GGCC 3 cut(s) 13, 23, 128
BtgI CCRYGG 1 cut(s) 345
BtsCI GGATG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 4 cut(s) 256, 352, 387, 490
CfoI GCGC 2 cut(s) 166, 494
Cfr13I GGNCC 1 cut(s) 469
Cfr9I CCCGGG 1 cut(s) 372
Csp6I GTAC 1 cut(s) 112
CviAII CATG 3 cut(s) 134, 251, 317
CviJI RGCY 9 cut(s) 13, 23, 28, 128, 217, 344, 389, 417, 548
CviKI_1 RGCY 9 cut(s) 13, 23, 28, 128, 217, 344, 389, 417, 548
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 2 cut(s) 167, 381
DraI TTTAAA 1 cut(s) 34
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 469
Eco47III AGCGCT 1 cut(s) 165
Eco88I CYCGRG 2 cut(s) 17, 372
EcoRII CCWGG 1 cut(s) 329
FaeI CATG 3 cut(s) 137, 254, 320
FaiI YATR 8 cut(s) 48, 97, 125, 135, 245, 252, 318, 454
FatI CATG 3 cut(s) 133, 250, 316
Fnu4HI GCNGC 1 cut(s) 563
FokI GGATG 1 cut(s) 287
Fsp4HI GCNGC 1 cut(s) 563
FspBI CTAG 3 cut(s) 446, 545, 571
GlaI GCGC 2 cut(s) 165, 493
GluI GCNGC 1 cut(s) 563
GsaI CCCAGC 1 cut(s) 487
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 3 cut(s) 13, 23, 128
HapII CCGG 1 cut(s) 373
HhaI GCGC 2 cut(s) 166, 494
Hin1II CATG 3 cut(s) 137, 254, 320
Hin6I GCGC 2 cut(s) 164, 492
HinP1I GCGC 2 cut(s) 164, 492
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 1 cut(s) 312
HpaII CCGG 1 cut(s) 373
HphI GGTGA 2 cut(s) 65, 317
Hpy166II GTNNAC 2 cut(s) 40, 114
Hpy188I TCNGA 2 cut(s) 157, 223
Hpy8I GTNNAC 2 cut(s) 40, 114
HpyAV CCTTC 2 cut(s) 148, 302
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 264, 386, 489, 500, 545
HpyF3I CTNAG 2 cut(s) 167, 381
Hsp92II CATG 3 cut(s) 137, 254, 320
HspAI GCGC 2 cut(s) 164, 492
LpnPI CCDG 7 cut(s) 142, 227, 268, 316, 343, 386, 469
LweI GCATC 4 cut(s) 109, 265, 267, 448
MaeI CTAG 3 cut(s) 446, 545, 571
MaeIII GTNAC 2 cut(s) 321, 356
MboII GAAGA 2 cut(s) 62, 71
MhlI GDGCHC 2 cut(s) 56, 490
MluCI AATT 1 cut(s) 205
MlyI GAGTC 1 cut(s) 306
MnlI CCTC 9 cut(s) 3, 13, 75, 98, 161, 198, 242, 295, 453
MseI TTAA 3 cut(s) 33, 180, 432
MspA1I CMGCKG 1 cut(s) 483
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 3 cut(s) 331, 373, 374
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 6 cut(s) 134, 264, 386, 489, 500, 545
NciI CCSGG 2 cut(s) 373, 374
NlaIII CATG 3 cut(s) 137, 254, 320
NlaIV GGNNCC 3 cut(s) 55, 176, 369
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 356
PkrI GCNGC 1 cut(s) 564
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 483
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 55, 176, 369
PspPI GGNCC 1 cut(s) 469
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SaqAI TTAA 3 cut(s) 33, 180, 432
SatI GCNGC 1 cut(s) 563
Sau96I GGNCC 1 cut(s) 469
SchI GAGTC 1 cut(s) 306
ScrFI CCNGG 3 cut(s) 331, 373, 374
SduI GDGCHC 2 cut(s) 56, 490
SetI ASST 7 cut(s) 78, 219, 335, 391, 419, 474, 509
SfaNI GCATC 4 cut(s) 109, 265, 267, 448
SinI GGWCC 1 cut(s) 469
SmaI CCCGGG 1 cut(s) 374
Sse9I AATT 1 cut(s) 205
SsiI CCGC 2 cut(s) 238, 481
SspI AATATT 1 cut(s) 84
SspMI CTAG 3 cut(s) 446, 545, 571
StyD4I CCNGG 3 cut(s) 329, 371, 372
TaaI ACNGT 1 cut(s) 327
TaqI TCGA 1 cut(s) 210
TasI AATT 1 cut(s) 205
Tru1I TTAA 3 cut(s) 33, 180, 432
Tru9I TTAA 3 cut(s) 33, 180, 432
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 1 cut(s) 562
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 2 cut(s) 174, 232
TspMI CCCGGG 1 cut(s) 372
TspRI CASTG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 469
XmaI CCCGGG 1 cut(s) 372
XspI CTAG 3 cut(s) 446, 545, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.