Rmu_sc0027953.1_g000002

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027953.1
OG0000863
Physical Location & Seq
Reverse (-)
469 .. 1278
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027953.1_g000002.1.cds

Sequence Viewer

Length: 810 bp
atggctaccagcacccccgatacacatgaagattatcagtctcgcgaaggaccaacgcgtgaggttcaacacaggctagaattcctcagcccaaacaaccaaacaccctcagtcatgacgaccgacgccacaaccgcacttaaaaccagaatgcaggacctcatggaatcgcgtgcgcaggcagagaccgagtgcacggcagctgcgacagcccatatggacatggaaaacatggccaatcagaaccgcattcttcgcgatgcactccagcgaagagtcgagaataatgaagcactcaatagggctcgccaacaagagaccccaatacctaccatcagcggtgtgaatacacctggggtcgtcctaaccacagatatgaatactagcttagctggcactttcatcccacccacatcaaaaacttctggaagcctcgcgagccctatactagggagcacaggcacagggccacaaatagtgtacctaaactcacctctctttcctaaccagctaggtatagcaccaccacctcttcccgaacttaatatggcaagcacatcgggcaccaacacaactatggccaccgccactgtgccctcacctgatcccaacacacaactgctaattcaaatgagcgagactatcactgccctacaggcacgaatcgagaatgcaaagagcagatctggctggcagtcgagaatgacgggattccaagggaaaatgggaccattcatacctcaaatacaggccgaaaaaagcccacaagaagcaaagcttttcaaaatagagaaatacaatggcacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

269

Amino Acids

29.19

Weight (kDa)

5.7

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000863)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29436 FvH4_4g08647
prunus_persica Prupe.1G244600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041081 RchiOBHm_Chr7g0227381
rosa_multiflora Rmu_co8094356.1_g000001 Rmu_co8097010.1_g000001 Rmu_co8307317.1_g000001 Rmu_co8331341.1_g000001 Rmu_co8353833.1_g000001 Rmu_co8426601.1_g000001 Rmu_co8464885.1_g000001 Rmu_sc0000161.1_g000045 Rmu_sc0000367.1_g000181 Rmu_sc0000438.1_g000020 Rmu_sc0000637.1_g000008 Rmu_sc0000706.1_g000036 Rmu_sc0000745.1_g000002 Rmu_sc0000964.1_g000007 Rmu_sc0001016.1_g000045 Rmu_sc0001190.1_g000046 Rmu_sc0002014.1_g000040 Rmu_sc0002229.1_g000012 Rmu_sc0002279.1_g000021 Rmu_sc0002279.1_g000049 Rmu_sc0002308.1_g000074 Rmu_sc0002482.1_g000017 Rmu_sc0002809.1_g000002 Rmu_sc0003147.1_g000020 Rmu_sc0003226.1_g000065 Rmu_sc0003246.1_g000004 Rmu_sc0003260.1_g000011 Rmu_sc0003441.1_g000081 Rmu_sc0003895.1_g000019 Rmu_sc0004228.1_g000001 Rmu_sc0004646.1_g000038 Rmu_sc0004688.1_g000018 Rmu_sc0005389.1_g000016 Rmu_sc0005537.1_g000011 Rmu_sc0005954.1_g000010 Rmu_sc0006021.1_g000020 Rmu_sc0006695.1_g000056 Rmu_sc0006822.1_g000010 Rmu_sc0007877.1_g000013 Rmu_sc0008049.1_g000067 Rmu_sc0008691.1_g000004 Rmu_sc0009206.1_g000002 Rmu_sc0011734.1_g000008 Rmu_sc0012136.1_g000012 Rmu_sc0012746.1_g000018 Rmu_sc0013612.1_g000011 Rmu_sc0016149.1_g000005 Rmu_sc0018867.1_g000011 Rmu_sc0020611.1_g000002 Rmu_sc0021291.1_g000004 Rmu_sc0021291.1_g000005 Rmu_sc0022400.1_g000007 Rmu_sc0025427.1_g000001 Rmu_sc0027953.1_g000002 Rmu_sc0033748.1_g000002 Rmu_ssc0000072.1_g000004 Rmu_ssc0000197.1_g000082
rosa_roxburghii Rroxscaffold_4G00282700 Rroxscaffold_5G00374470 Rroxscaffold_7G00183200
rosa_rugosa Rorug02G0187100 Rorug05G0255100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 563
AccII CGCG 5 cut(s) 45, 58, 172, 258, 437
AciI CCGC 4 cut(s) 135, 247, 339, 585
AclWI GGATC 1 cut(s) 599
AcoI YGGCCR 2 cut(s) 234, 579
AcsI RAATTY 1 cut(s) 80
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 3 cut(s) 68, 629, 784
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 5 cut(s) 203, 387, 392, 511, 778
AluI AGCT 5 cut(s) 203, 387, 392, 511, 778
Alw21I GWGCWC 2 cut(s) 197, 458
Alw26I GTCTC 4 cut(s) 45, 179, 311, 632
Alw44I GTGCAC 1 cut(s) 193
AlwI GGATC 1 cut(s) 599
AoxI GGCC 4 cut(s) 234, 467, 579, 750
ApaLI GTGCAC 1 cut(s) 193
ApeKI GCWGC 2 cut(s) 200, 203
ApoI RAATTY 1 cut(s) 80
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 4 cut(s) 50, 157, 467, 728
AsuHPI GGTGA 2 cut(s) 483, 591
AvaII GGWCC 3 cut(s) 50, 157, 728
BaeGI GKGCMC 3 cut(s) 197, 566, 597
BalI TGGCCA 2 cut(s) 236, 581
BanI GGYRCC 1 cut(s) 563
BanII GRGCYC 2 cut(s) 307, 443
Bbv12I GWGCWC 2 cut(s) 197, 458
BbvCI CCTCAGC 1 cut(s) 86
BbvI GCAGC 2 cut(s) 190, 212
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 2 cut(s) 540, 574
BciT130I CCWGG 1 cut(s) 354
BcoDI GTCTC 4 cut(s) 45, 179, 311, 632
BfaI CTAG 4 cut(s) 77, 384, 449, 512
BfmI CTRYAG 1 cut(s) 653
BglI GCCNNNNNGGC 1 cut(s) 656
BglII AGATCT 1 cut(s) 683
BisI GCNGC 2 cut(s) 201, 204
BlpI GCTNAGC 1 cut(s) 388
BlsI GCNGC 2 cut(s) 202, 205
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 3 cut(s) 50, 157, 728
BmgT120I GGNCC 4 cut(s) 50, 157, 467, 728
BmiI GGNNCC 2 cut(s) 565, 729
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 1 cut(s) 86
Bpu1102I GCTNAGC 1 cut(s) 388
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 2 cut(s) 179, 311
BsaJI CCNNGG 2 cut(s) 353, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 449
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 353, 715
BseGI GGATG 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMII CTCAG 2 cut(s) 100, 123
BseSI GKGCMC 3 cut(s) 197, 566, 597
BseXI GCAGC 2 cut(s) 190, 212
Bsh1236I CGCG 5 cut(s) 45, 58, 172, 258, 437
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 4 cut(s) 236, 469, 581, 752
BshNI GGYRCC 1 cut(s) 563
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 2 cut(s) 197, 458
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 4 cut(s) 45, 179, 311, 632
BsmFI GGGAC 1 cut(s) 741
BsmI GAATGC 3 cut(s) 156, 249, 676
BsnI GGCC 4 cut(s) 236, 469, 581, 752
Bso31I GGTCTC 2 cut(s) 179, 311
Bsp1286I GDGCHC 6 cut(s) 197, 307, 443, 458, 566, 597
Bsp143I GATC 2 cut(s) 604, 683
Bsp1720I GCTNAGC 1 cut(s) 388
Bsp68I TCGCGA 3 cut(s) 45, 258, 437
BspACI CCGC 4 cut(s) 135, 247, 339, 585
BspANI GGCC 4 cut(s) 236, 469, 581, 752
BspCNI CTCAG 2 cut(s) 99, 122
BspFNI CGCG 5 cut(s) 45, 58, 172, 258, 437
BspHI TCATGA 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 565, 729
BspPI GGATC 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 563
BspTNI GGTCTC 2 cut(s) 179, 311
BssECI CCNNGG 2 cut(s) 353, 715
BssMI GATC 2 cut(s) 604, 683
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 715
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 2 cut(s) 268, 537
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 7 cut(s) 174, 180, 307, 394, 439, 553, 692
BstDEI CTNAG 3 cut(s) 86, 109, 388
BstENI CCTNNNNNAGG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 402
BstFNI CGCG 5 cut(s) 45, 58, 172, 258, 437
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 2 cut(s) 607, 686
BstMAI GTCTC 4 cut(s) 45, 179, 311, 632
BstMBI GATC 2 cut(s) 604, 683
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 8 cut(s) 134, 209, 255, 393, 438, 561, 656, 687
BstNI CCWGG 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 1 cut(s) 653
BstSLI GKGCMC 3 cut(s) 197, 566, 597
BstUI CGCG 5 cut(s) 45, 58, 172, 258, 437
BstV1I GCAGC 2 cut(s) 190, 212
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BsuRI GGCC 4 cut(s) 236, 469, 581, 752
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 402
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 2 cut(s) 588, 645
BtuMI TCGCGA 3 cut(s) 45, 258, 437
Cac8I GCNNGC 7 cut(s) 174, 180, 307, 394, 439, 553, 692
CciI TCATGA 1 cut(s) 114
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 4 cut(s) 50, 157, 467, 728
CseI GACGC 1 cut(s) 134
Csp6I GTAC 1 cut(s) 481
CviAII CATG 6 cut(s) 26, 115, 163, 223, 232, 807
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 3 cut(s) 86, 109, 388
DpnI GATC 2 cut(s) 606, 685
DpnII GATC 2 cut(s) 604, 683
EaeI YGGCCR 2 cut(s) 234, 579
Eam1104I CTCTTC 2 cut(s) 268, 537
EarI CTCTTC 2 cut(s) 268, 537
Eco130I CCWWGG 1 cut(s) 715
Eco24I GRGCYC 2 cut(s) 307, 443
Eco31I GGTCTC 2 cut(s) 179, 311
Eco47I GGWCC 3 cut(s) 50, 157, 728
EcoNI CCTNNNNNAGG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 157
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 1 cut(s) 715
EcoT38I GRGCYC 2 cut(s) 307, 443
ErhI CCWWGG 1 cut(s) 715
FaeI CATG 6 cut(s) 29, 118, 166, 226, 235, 810
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 1 cut(s) 741
FatI CATG 6 cut(s) 25, 114, 162, 222, 231, 806
FauNDI CATATG 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 201, 204
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 2 cut(s) 307, 443
Fsp4HI GCNGC 2 cut(s) 201, 204
FspBI CTAG 4 cut(s) 77, 384, 449, 512
FspI TGCGCA 1 cut(s) 177
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 201, 204
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 4 cut(s) 236, 469, 581, 752
HgaI GACGC 1 cut(s) 134
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 6 cut(s) 29, 118, 166, 226, 235, 810
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 4 cut(s) 167, 276, 663, 711
HphI GGTGA 2 cut(s) 483, 591
Hpy166II GTNNAC 2 cut(s) 195, 481
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 9 cut(s) 44, 115, 257, 280, 426, 436, 536, 667, 699
Hpy8I GTNNAC 2 cut(s) 195, 481
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4V TGCA 4 cut(s) 154, 195, 263, 674
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 209, 255, 393, 438, 561, 656, 687
HpyF3I CTNAG 3 cut(s) 86, 109, 388
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 6 cut(s) 29, 118, 166, 226, 235, 810
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 2 cut(s) 604, 683
LmnI GCTCC 1 cut(s) 453
Lsp1109I GCAGC 2 cut(s) 190, 212
LweI GCATC 1 cut(s) 250
MaeI CTAG 4 cut(s) 77, 384, 449, 512
MalI GATC 2 cut(s) 606, 685
MboI GATC 2 cut(s) 604, 683
MboII GAAGA 4 cut(s) 41, 245, 285, 524
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 6 cut(s) 197, 307, 443, 458, 566, 597
MlsI TGGCCA 2 cut(s) 236, 581
MluCI AATT 2 cut(s) 80, 624
MluI ACGCGT 1 cut(s) 56
MluNI TGGCCA 2 cut(s) 236, 581
MlyI GAGTC 1 cut(s) 285
MnlI CCTC 9 cut(s) 55, 95, 118, 170, 443, 504, 540, 607, 750
Mox20I TGGCCA 2 cut(s) 236, 581
MscI TGGCCA 2 cut(s) 236, 581
MseI TTAA 2 cut(s) 141, 543
MslI CAYNNNNRTG 2 cut(s) 374, 575
Msp20I TGGCCA 2 cut(s) 236, 581
MspA1I CMGCKG 2 cut(s) 203, 339
MspR9I CCNGG 1 cut(s) 354
Mva1269I GAATGC 3 cut(s) 156, 249, 676
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 5 cut(s) 45, 58, 172, 258, 437
MwoI GCNNNNNNNGC 8 cut(s) 134, 209, 255, 393, 438, 561, 656, 687
NdeI CATATG 1 cut(s) 216
NdeII GATC 2 cut(s) 604, 683
NlaIII CATG 6 cut(s) 29, 118, 166, 226, 235, 810
NlaIV GGNNCC 2 cut(s) 565, 729
NruI TCGCGA 3 cut(s) 45, 258, 437
NsbI TGCGCA 1 cut(s) 177
PagI TCATGA 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 3 cut(s) 156, 249, 676
PfeI GAWTC 3 cut(s) 167, 663, 711
PkrI GCNGC 2 cut(s) 202, 205
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 157
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 2 cut(s) 565, 729
PspPI GGNCC 4 cut(s) 50, 157, 467, 728
PspPPI RGGWCCY 1 cut(s) 157
PsuI RGATCY 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 203
RruI TCGCGA 3 cut(s) 45, 258, 437
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
RseI CAYNNNNRTG 2 cut(s) 374, 575
SaqAI TTAA 2 cut(s) 141, 543
SatI GCNGC 2 cut(s) 201, 204
Sau3AI GATC 2 cut(s) 604, 683
Sau96I GGNCC 4 cut(s) 50, 157, 467, 728
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 6 cut(s) 197, 307, 443, 458, 566, 597
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 653
SinI GGWCC 3 cut(s) 50, 157, 728
SmiMI CAYNNNNRTG 2 cut(s) 374, 575
Sse9I AATT 2 cut(s) 80, 624
SsiI CCGC 4 cut(s) 135, 247, 339, 585
SspMI CTAG 4 cut(s) 77, 384, 449, 512
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 715
TaaI ACNGT 1 cut(s) 592
TaqI TCGA 3 cut(s) 279, 666, 698
TaqII GACCGA 2 cut(s) 137, 203
TasI AATT 2 cut(s) 80, 624
TfiI GAWTC 3 cut(s) 167, 663, 711
Tru1I TTAA 2 cut(s) 141, 543
Tru9I TTAA 2 cut(s) 141, 543
TscAI CASTG 2 cut(s) 595, 652
TseI GCWGC 2 cut(s) 200, 203
TspDTI ATGAA 5 cut(s) 42, 303, 391, 392, 724
TspRI CASTG 2 cut(s) 595, 652
VneI GTGCAC 1 cut(s) 193
VpaK11BI GGWCC 3 cut(s) 50, 157, 728
XagI CCTNNNNNAGG 1 cut(s) 447
XapI RAATTY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XspI CTAG 4 cut(s) 77, 384, 449, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.