Rmu_ssc0000072.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000072.1
OG0000863
Physical Location & Seq
Forward (+)
14458 .. 15039
582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000072.1_g000004.1.cds

Sequence Viewer

Length: 582 bp
atggctactagtaataacgataccagcagcgacgatctccatagtgagacaacgatccgcgaggtccaacgtattctcgagtttcagagcccgaacaccgagactcaattggtcatgactaccgatccaattactgtgctacagatagcgatgcgcgacctcatggagacgtgtgcacaggtcgaggcagagcgcgcagcggccgtggcagcccccgaggaagtcaaaaatataaccaacaataatcggaccctactagaggcactccagctgggggtcgcgcatgacgacaccctggacagggcccacacgcaggaaaccccgataccgaccattggtggtgtccccacaccgggacaggcgctcactgcggaccccgccaccaacctcgctggcgcctccatgccccgaaaccccgcatctggaagtcccgctggccccacgcatggcaacaccagcacagaccctcaagtggtctacctgaactcccttctctttccaggcagccgccggtgcagcggggactttagatgccatcactgctacgactaccaacatacctctacccaatcccaatactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

20.83

Weight (kDa)

4.78

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000863)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29436 FvH4_4g08647
prunus_persica Prupe.1G244600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041081 RchiOBHm_Chr7g0227381
rosa_multiflora Rmu_co8094356.1_g000001 Rmu_co8097010.1_g000001 Rmu_co8307317.1_g000001 Rmu_co8331341.1_g000001 Rmu_co8353833.1_g000001 Rmu_co8426601.1_g000001 Rmu_co8464885.1_g000001 Rmu_sc0000161.1_g000045 Rmu_sc0000367.1_g000181 Rmu_sc0000438.1_g000020 Rmu_sc0000637.1_g000008 Rmu_sc0000706.1_g000036 Rmu_sc0000745.1_g000002 Rmu_sc0000964.1_g000007 Rmu_sc0001016.1_g000045 Rmu_sc0001190.1_g000046 Rmu_sc0002014.1_g000040 Rmu_sc0002229.1_g000012 Rmu_sc0002279.1_g000021 Rmu_sc0002279.1_g000049 Rmu_sc0002308.1_g000074 Rmu_sc0002482.1_g000017 Rmu_sc0002809.1_g000002 Rmu_sc0003147.1_g000020 Rmu_sc0003226.1_g000065 Rmu_sc0003246.1_g000004 Rmu_sc0003260.1_g000011 Rmu_sc0003441.1_g000081 Rmu_sc0003895.1_g000019 Rmu_sc0004228.1_g000001 Rmu_sc0004646.1_g000038 Rmu_sc0004688.1_g000018 Rmu_sc0005389.1_g000016 Rmu_sc0005537.1_g000011 Rmu_sc0005954.1_g000010 Rmu_sc0006021.1_g000020 Rmu_sc0006695.1_g000056 Rmu_sc0006822.1_g000010 Rmu_sc0007877.1_g000013 Rmu_sc0008049.1_g000067 Rmu_sc0008691.1_g000004 Rmu_sc0009206.1_g000002 Rmu_sc0011734.1_g000008 Rmu_sc0012136.1_g000012 Rmu_sc0012746.1_g000018 Rmu_sc0013612.1_g000011 Rmu_sc0016149.1_g000005 Rmu_sc0018867.1_g000011 Rmu_sc0020611.1_g000002 Rmu_sc0021291.1_g000004 Rmu_sc0021291.1_g000005 Rmu_sc0022400.1_g000007 Rmu_sc0025427.1_g000001 Rmu_sc0027953.1_g000002 Rmu_sc0033748.1_g000002 Rmu_ssc0000072.1_g000004 Rmu_ssc0000197.1_g000082
rosa_roxburghii Rroxscaffold_4G00282700 Rroxscaffold_5G00374470 Rroxscaffold_7G00183200
rosa_rugosa Rorug02G0187100 Rorug05G0255100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 395
AccI GTMKAC 1 cut(s) 477
AccII CGCG 4 cut(s) 60, 156, 195, 281
AciI CCGC 8 cut(s) 58, 200, 371, 378, 417, 432, 508, 519
AclWI GGATC 2 cut(s) 49, 119
AcoI YGGCCR 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 396
AflIII ACRYGT 1 cut(s) 170
AhlI ACTAGT 1 cut(s) 8
AjiI CACGTC 1 cut(s) 171
AjnI CCWGG 2 cut(s) 294, 499
AluBI AGCT 1 cut(s) 271
AluI AGCT 1 cut(s) 271
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 3 cut(s) 41, 95, 161
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 2 cut(s) 49, 119
Ama87I CYCGRG 2 cut(s) 77, 215
AoxI GGCC 3 cut(s) 201, 303, 436
ApaI GGGCCC 1 cut(s) 307
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 5 cut(s) 27, 197, 209, 504, 516
AspLEI GCGC 6 cut(s) 156, 195, 197, 283, 364, 398
AspS9I GGNCC 6 cut(s) 64, 249, 303, 304, 373, 437
AsuC2I CCSGG 1 cut(s) 354
AvaI CYCGRG 2 cut(s) 77, 215
AvaII GGWCC 3 cut(s) 64, 249, 373
BaeGI GKGCMC 2 cut(s) 178, 307
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 2 cut(s) 92, 307
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 5 cut(s) 39, 209, 221, 516, 528
BccI CCATC 1 cut(s) 543
BceAI ACGGC 1 cut(s) 188
BciT130I CCWGG 2 cut(s) 296, 501
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 3 cut(s) 41, 95, 161
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 3 cut(s) 9, 257, 580
BfmI CTRYAG 1 cut(s) 140
BfoI RGCGCY 2 cut(s) 365, 399
BisI GCNGC 7 cut(s) 28, 198, 201, 210, 505, 508, 517
BlsI GCNGC 7 cut(s) 29, 199, 202, 211, 506, 509, 518
Bme1390I CCNGG 3 cut(s) 296, 354, 501
Bme18I GGWCC 3 cut(s) 64, 249, 373
BmeT110I CYCGRG 2 cut(s) 77, 215
BmgBI CACGTC 1 cut(s) 171
BmgT120I GGNCC 6 cut(s) 64, 249, 303, 304, 373, 437
BmiI GGNNCC 5 cut(s) 251, 305, 375, 397, 439
BmrFI CCNGG 3 cut(s) 296, 354, 501
BmsI GCATC 3 cut(s) 141, 428, 521
BpmI CTGGAG 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 453
BpuMI CCSGG 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 204, 216, 294
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bse118I RCCGGY 1 cut(s) 510
BseBI CCWGG 2 cut(s) 296, 501
BseDI CCNNGG 3 cut(s) 204, 216, 294
BsePI GCGCGC 1 cut(s) 193
BseSI GKGCMC 2 cut(s) 178, 307
BseX3I CGGCCG 1 cut(s) 201
BseXI GCAGC 5 cut(s) 39, 209, 221, 516, 528
BseYI CCCAGC 1 cut(s) 271
BsgI GTGCAG 1 cut(s) 535
Bsh1236I CGCG 4 cut(s) 60, 156, 195, 281
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 3 cut(s) 203, 305, 438
BshNI GGYRCC 1 cut(s) 395
BsiEI CGRYCG 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 178
BsiHKCI CYCGRG 2 cut(s) 77, 215
BsiSI CCGG 2 cut(s) 353, 511
BslFI GGGAC 4 cut(s) 329, 369, 414, 536
BsmAI GTCTC 3 cut(s) 41, 95, 161
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 4 cut(s) 329, 369, 414, 536
BsnI GGCC 3 cut(s) 203, 305, 438
BsoBI CYCGRG 2 cut(s) 77, 215
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 3 cut(s) 92, 178, 307
Bsp143I GATC 3 cut(s) 34, 54, 124
BspACI CCGC 8 cut(s) 58, 200, 371, 378, 417, 432, 508, 519
BspANI GGCC 3 cut(s) 203, 305, 438
BspFNI CGCG 4 cut(s) 60, 156, 195, 281
BspHI TCATGA 1 cut(s) 114
BspLI GGNNCC 5 cut(s) 251, 305, 375, 397, 439
BspPI GGATC 2 cut(s) 49, 119
BspT107I GGYRCC 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 3 cut(s) 204, 216, 294
BssHII GCGCGC 1 cut(s) 193
BssMI GATC 3 cut(s) 34, 54, 124
BssNI GRCGYC 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 296, 501
Bst4CI ACNGT 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 3 cut(s) 195, 394, 436
BstDSI CCRYGG 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 257
BstFNI CGCG 4 cut(s) 60, 156, 195, 281
BstH2I RGCGCY 2 cut(s) 365, 399
BstHHI GCGC 6 cut(s) 156, 195, 197, 283, 364, 398
BstKTI GATC 3 cut(s) 37, 57, 127
BstMAI GTCTC 3 cut(s) 41, 95, 161
BstMBI GATC 3 cut(s) 34, 54, 124
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 9 cut(s) 194, 206, 209, 368, 377, 456, 513, 516, 540
BstNI CCWGG 2 cut(s) 296, 501
BstSCI CCNGG 3 cut(s) 294, 352, 499
BstSFI CTRYAG 1 cut(s) 140
BstSLI GKGCMC 2 cut(s) 178, 307
BstUI CGCG 4 cut(s) 60, 156, 195, 281
BstV1I GCAGC 5 cut(s) 39, 209, 221, 516, 528
BstZI CGGCCG 1 cut(s) 201
BsuRI GGCC 3 cut(s) 203, 305, 438
BtgI CCRYGG 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 164
BtrI CACGTC 1 cut(s) 171
BtsI GCAGTG 2 cut(s) 366, 538
BtsIMutI CAGTG 2 cut(s) 366, 538
Cac8I GCNNGC 3 cut(s) 195, 394, 436
CciI TCATGA 1 cut(s) 114
CfoI GCGC 6 cut(s) 156, 195, 197, 283, 364, 398
Cfr10I RCCGGY 1 cut(s) 510
Cfr13I GGNCC 6 cut(s) 64, 249, 303, 304, 373, 437
CviAII CATG 5 cut(s) 115, 163, 284, 403, 446
CviJI RGCY 8 cut(s) 5, 90, 203, 212, 271, 305, 438, 507
CviKI_1 RGCY 8 cut(s) 5, 90, 203, 212, 271, 305, 438, 507
DinI GGCGCC 1 cut(s) 397
DpnI GATC 3 cut(s) 36, 56, 126
DpnII GATC 3 cut(s) 34, 54, 124
EaeI YGGCCR 1 cut(s) 201
EagI CGGCCG 1 cut(s) 201
EclXI CGGCCG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 92, 307
Eco47I GGWCC 3 cut(s) 64, 249, 373
Eco52I CGGCCG 1 cut(s) 201
Eco88I CYCGRG 2 cut(s) 77, 215
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 294, 499
EcoT38I GRGCYC 2 cut(s) 92, 307
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 5 cut(s) 118, 166, 287, 406, 449
FaiI YATR 8 cut(s) 42, 116, 164, 233, 285, 404, 447, 558
FaqI GGGAC 4 cut(s) 329, 369, 414, 536
FatI CATG 5 cut(s) 114, 162, 283, 402, 445
FauI CCCGC 4 cut(s) 385, 424, 439, 512
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 7 cut(s) 28, 198, 201, 210, 505, 508, 517
FriOI GRGCYC 2 cut(s) 92, 307
Fsp4HI GCNGC 7 cut(s) 28, 198, 201, 210, 505, 508, 517
FspBI CTAG 3 cut(s) 9, 257, 580
GlaI GCGC 6 cut(s) 155, 194, 196, 282, 363, 397
GluI GCNGC 7 cut(s) 28, 198, 201, 210, 505, 508, 517
GsaI CCCAGC 1 cut(s) 275
GsuI CTGGAG 1 cut(s) 251
HaeII RGCGCY 2 cut(s) 365, 399
HaeIII GGCC 3 cut(s) 203, 305, 438
HapII CCGG 2 cut(s) 353, 511
HhaI GCGC 6 cut(s) 156, 195, 197, 283, 364, 398
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 5 cut(s) 118, 166, 287, 406, 449
Hin6I GCGC 6 cut(s) 154, 193, 195, 281, 362, 396
HinP1I GCGC 6 cut(s) 154, 193, 195, 281, 362, 396
HinfI GANTC 1 cut(s) 103
HpaII CCGG 2 cut(s) 353, 511
Hpy166II GTNNAC 2 cut(s) 176, 478
Hpy188I TCNGA 2 cut(s) 87, 249
Hpy188III TCNNGA 3 cut(s) 77, 115, 423
Hpy8I GTNNAC 2 cut(s) 176, 478
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 1 cut(s) 500
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 70, 170
HpyCH4V TGCA 2 cut(s) 176, 516
HpyF10VI GCNNNNNNNGC 9 cut(s) 194, 206, 209, 368, 377, 456, 513, 516, 540
HpySE526I ACGT 2 cut(s) 70, 170
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 5 cut(s) 118, 166, 287, 406, 449
HspAI GCGC 6 cut(s) 154, 193, 195, 281, 362, 396
KasI GGCGCC 1 cut(s) 395
Kzo9I GATC 3 cut(s) 34, 54, 124
Lsp1109I GCAGC 5 cut(s) 39, 209, 221, 516, 528
LweI GCATC 3 cut(s) 141, 428, 521
MaeI CTAG 3 cut(s) 9, 257, 580
MaeII ACGT 2 cut(s) 70, 170
MalI GATC 3 cut(s) 36, 56, 126
MboI GATC 3 cut(s) 34, 54, 124
MfeI CAATTG 1 cut(s) 107
MhlI GDGCHC 3 cut(s) 92, 178, 307
MluCI AATT 2 cut(s) 107, 129
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 9 cut(s) 55, 170, 178, 211, 253, 398, 409, 477, 571
MspA1I CMGCKG 4 cut(s) 200, 271, 434, 519
MspI CCGG 2 cut(s) 353, 511
MspR9I CCNGG 3 cut(s) 296, 354, 501
MunI CAATTG 1 cut(s) 107
MvaI CCWGG 2 cut(s) 296, 501
MvnI CGCG 4 cut(s) 60, 156, 195, 281
MwoI GCNNNNNNNGC 9 cut(s) 194, 206, 209, 368, 377, 456, 513, 516, 540
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 1 cut(s) 354
NdeII GATC 3 cut(s) 34, 54, 124
NlaIII CATG 5 cut(s) 118, 166, 287, 406, 449
NlaIV GGNNCC 5 cut(s) 251, 305, 375, 397, 439
PaeR7I CTCGAG 1 cut(s) 77
PagI TCATGA 1 cut(s) 114
PauI GCGCGC 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PkrI GCNGC 7 cut(s) 29, 199, 202, 211, 506, 509, 518
PleI GAGTC 1 cut(s) 97
PluTI GGCGCC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 294, 499
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 2 cut(s) 294, 499
PspN4I GGNNCC 5 cut(s) 251, 305, 375, 397, 439
PspOMI GGGCCC 1 cut(s) 303
PspPI GGNCC 6 cut(s) 64, 249, 303, 304, 373, 437
PteI GCGCGC 1 cut(s) 193
PvuII CAGCTG 1 cut(s) 271
SatI GCNGC 7 cut(s) 28, 198, 201, 210, 505, 508, 517
Sau3AI GATC 3 cut(s) 34, 54, 124
Sau96I GGNCC 6 cut(s) 64, 249, 303, 304, 373, 437
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 3 cut(s) 296, 354, 501
SduI GDGCHC 3 cut(s) 92, 178, 307
SetI ASST 9 cut(s) 66, 73, 162, 173, 183, 273, 390, 483, 563
SfaNI GCATC 3 cut(s) 141, 428, 521
SfcI CTRYAG 1 cut(s) 140
SfoI GGCGCC 1 cut(s) 397
Sfr274I CTCGAG 1 cut(s) 77
SgrAI CRCCGGYG 1 cut(s) 510
SinI GGWCC 3 cut(s) 64, 249, 373
SlaI CTCGAG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 77, 468
SmoI CTYRAG 2 cut(s) 77, 468
SpeI ACTAGT 1 cut(s) 8
Sse9I AATT 2 cut(s) 107, 129
SsiI CCGC 8 cut(s) 58, 200, 371, 378, 417, 432, 508, 519
SspDI GGCGCC 1 cut(s) 395
SspMI CTAG 3 cut(s) 9, 257, 580
StyD4I CCNGG 3 cut(s) 294, 352, 499
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 73, 173
TaqI TCGA 2 cut(s) 78, 183
TasI AATT 2 cut(s) 107, 129
TauI GCSGC 2 cut(s) 203, 510
TscAI CASTG 2 cut(s) 373, 545
TseI GCWGC 5 cut(s) 27, 197, 209, 504, 516
TspRI CASTG 2 cut(s) 373, 545
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 3 cut(s) 64, 249, 373
XagI CCTNNNNNAGG 1 cut(s) 257
XhoI CTCGAG 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 3 cut(s) 9, 257, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.