Rroxscaffold_4G00282700

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000863
Physical Location & Seq
Forward (+)
4813035 .. 4814843
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282700.1

Sequence Viewer

Length: 447 bp
ATGGCATGTCATGCATTCGAGGAAACCCTAACCGACGAGGCCTTGCGTTGGTTTCTCAATCTTCCTCCCAACTTCATCGATAGCTTCCGGGAGCTTGGGGAAAAATTTCTCCGCATATTCATTCCGTGCGGTAGCGGCTACCGCACGACACCGATCTATTCGAGCTTAAACGGCGGCCAAATGAACTATTACGAGATTTCATCCGCCGCCGGCGTAAAACAAGTCGCTCGTTGTCGTTCTCTTGACCCGGCCTCTTGCGCATTGGCATTCAAACAAGCACTAAAGCTAGGATACTTCCTACTACAAATCAACACCAATCCCCCGCGACATATGATGATCTCCTCGATGCCGCTACCGCCTTCGCCTGTGCCGAATAAGATACTTTTGGTCGCGATACTAGCTCTAGTGCGCACCCGGCTAGTGTCCCTCAAGCCAACAACCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

148

Amino Acids

16.49

Weight (kDa)

9.39

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000863)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29436 FvH4_4g08647
prunus_persica Prupe.1G244600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041081 RchiOBHm_Chr7g0227381
rosa_multiflora Rmu_co8094356.1_g000001 Rmu_co8097010.1_g000001 Rmu_co8307317.1_g000001 Rmu_co8331341.1_g000001 Rmu_co8353833.1_g000001 Rmu_co8426601.1_g000001 Rmu_co8464885.1_g000001 Rmu_sc0000161.1_g000045 Rmu_sc0000367.1_g000181 Rmu_sc0000438.1_g000020 Rmu_sc0000637.1_g000008 Rmu_sc0000706.1_g000036 Rmu_sc0000745.1_g000002 Rmu_sc0000964.1_g000007 Rmu_sc0001016.1_g000045 Rmu_sc0001190.1_g000046 Rmu_sc0002014.1_g000040 Rmu_sc0002229.1_g000012 Rmu_sc0002279.1_g000021 Rmu_sc0002279.1_g000049 Rmu_sc0002308.1_g000074 Rmu_sc0002482.1_g000017 Rmu_sc0002809.1_g000002 Rmu_sc0003147.1_g000020 Rmu_sc0003226.1_g000065 Rmu_sc0003246.1_g000004 Rmu_sc0003260.1_g000011 Rmu_sc0003441.1_g000081 Rmu_sc0003895.1_g000019 Rmu_sc0004228.1_g000001 Rmu_sc0004646.1_g000038 Rmu_sc0004688.1_g000018 Rmu_sc0005389.1_g000016 Rmu_sc0005537.1_g000011 Rmu_sc0005954.1_g000010 Rmu_sc0006021.1_g000020 Rmu_sc0006695.1_g000056 Rmu_sc0006822.1_g000010 Rmu_sc0007877.1_g000013 Rmu_sc0008049.1_g000067 Rmu_sc0008691.1_g000004 Rmu_sc0009206.1_g000002 Rmu_sc0011734.1_g000008 Rmu_sc0012136.1_g000012 Rmu_sc0012746.1_g000018 Rmu_sc0013612.1_g000011 Rmu_sc0016149.1_g000005 Rmu_sc0018867.1_g000011 Rmu_sc0020611.1_g000002 Rmu_sc0021291.1_g000004 Rmu_sc0021291.1_g000005 Rmu_sc0022400.1_g000007 Rmu_sc0025427.1_g000001 Rmu_sc0027953.1_g000002 Rmu_sc0033748.1_g000002 Rmu_ssc0000072.1_g000004 Rmu_ssc0000197.1_g000082
rosa_roxburghii Rroxscaffold_4G00282700 Rroxscaffold_5G00374470 Rroxscaffold_7G00183200
rosa_rugosa Rorug02G0187100 Rorug05G0255100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 259, 410
AccII CGCG 2 cut(s) 325, 392
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 48
AgsI TTSAA 1 cut(s) 271
AluBI AGCT 5 cut(s) 84, 94, 165, 286, 401
AluI AGCT 5 cut(s) 84, 94, 165, 286, 401
AoxI GGCC 3 cut(s) 39, 175, 249
ApoI RAATTY 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 2 cut(s) 260, 411
AsuC2I CCSGG 3 cut(s) 89, 248, 415
BceAI ACGGC 1 cut(s) 187
BciVI GTATCC 1 cut(s) 284
BcnI CCSGG 3 cut(s) 89, 248, 415
BfaI CTAG 4 cut(s) 287, 398, 404, 419
BfuI GTATCC 1 cut(s) 284
BisI GCNGC 4 cut(s) 136, 175, 207, 350
BlsI GCNGC 4 cut(s) 137, 176, 208, 351
Bme1390I CCNGG 3 cut(s) 89, 248, 415
BmrFI CCNGG 3 cut(s) 89, 248, 415
BmsI GCATC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 3 cut(s) 89, 248, 415
Bsa29I ATCGAT 1 cut(s) 78
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse118I RCCGGY 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 78
BseGI GGATG 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 331
Bsh1236I CGCG 2 cut(s) 325, 392
BshFI GGCC 3 cut(s) 41, 177, 251
BshVI ATCGAT 1 cut(s) 78
BsiSI CCGG 4 cut(s) 88, 210, 248, 415
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 2 cut(s) 14, 266
BsnI GGCC 3 cut(s) 41, 177, 251
Bsp143I GATC 2 cut(s) 153, 336
Bsp68I TCGCGA 1 cut(s) 392
BspANI GGCC 3 cut(s) 41, 177, 251
BspDI ATCGAT 1 cut(s) 78
BspFNI CGCG 2 cut(s) 325, 392
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssMI GATC 2 cut(s) 153, 336
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 211
BstF5I GGATG 1 cut(s) 200
BstFNI CGCG 2 cut(s) 325, 392
BstHHI GCGC 2 cut(s) 260, 411
BstKTI GATC 2 cut(s) 156, 339
BstMBI GATC 2 cut(s) 153, 336
BstMWI GCNNNNNNNGC 8 cut(s) 11, 135, 141, 171, 257, 355, 398, 415
BstNSI RCATGY 1 cut(s) 9
BstSCI CCNGG 3 cut(s) 87, 246, 413
BstUI CGCG 2 cut(s) 325, 392
Bsu15I ATCGAT 1 cut(s) 78
BsuI GTATCC 1 cut(s) 284
BsuRI GGCC 3 cut(s) 41, 177, 251
BsuTUI ATCGAT 1 cut(s) 78
BtsCI GGATG 1 cut(s) 200
BtuMI TCGCGA 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 211
CfoI GCGC 2 cut(s) 260, 411
Cfr10I RCCGGY 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 78
CviAII CATG 2 cut(s) 6, 11
DpnI GATC 2 cut(s) 155, 338
DpnII GATC 2 cut(s) 153, 336
EaeI YGGCCR 1 cut(s) 175
EciI GGCGGA 1 cut(s) 193
Eco147I AGGCCT 1 cut(s) 41
EcoT22I ATGCAT 1 cut(s) 16
FaeI CATG 2 cut(s) 9, 14
FaiI YATR 6 cut(s) 7, 12, 116, 330, 332, 445
FaqI GGGAC 1 cut(s) 409
FatI CATG 2 cut(s) 5, 10
FauI CCCGC 1 cut(s) 330
FauNDI CATATG 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 136, 175, 207, 350
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 4 cut(s) 136, 175, 207, 350
FspAI RTGCGCAY 1 cut(s) 410
FspBI CTAG 4 cut(s) 287, 398, 404, 419
FspI TGCGCA 2 cut(s) 259, 410
GlaI GCGC 2 cut(s) 259, 410
GluI GCNGC 4 cut(s) 136, 175, 207, 350
HaeIII GGCC 3 cut(s) 41, 177, 251
HapII CCGG 4 cut(s) 88, 210, 248, 415
HhaI GCGC 2 cut(s) 260, 411
Hin1II CATG 2 cut(s) 9, 14
Hin6I GCGC 2 cut(s) 258, 409
HinP1I GCGC 2 cut(s) 258, 409
HpaII CCGG 4 cut(s) 88, 210, 248, 415
Hpy188III TCNNGA 2 cut(s) 242, 391
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 1 cut(s) 369
HpyCH4V TGCA 1 cut(s) 14
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 135, 141, 171, 257, 355, 398, 415
Hsp92II CATG 2 cut(s) 9, 14
HspAI GCGC 2 cut(s) 258, 409
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 153, 336
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 5 cut(s) 101, 223, 261, 378, 428
LweI GCATC 1 cut(s) 336
MaeI CTAG 4 cut(s) 287, 398, 404, 419
MalI GATC 2 cut(s) 155, 338
MboI GATC 2 cut(s) 153, 336
MboII GAAGA 1 cut(s) 53
MluCI AATT 1 cut(s) 104
MnlI CCTC 6 cut(s) 13, 31, 75, 262, 352, 437
Mph1103I ATGCAT 1 cut(s) 16
MreI CGCCGGCG 1 cut(s) 209
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 1 cut(s) 167
MspI CCGG 4 cut(s) 88, 210, 248, 415
MspR9I CCNGG 3 cut(s) 89, 248, 415
Mva1269I GAATGC 2 cut(s) 14, 266
MvnI CGCG 2 cut(s) 325, 392
MwoI GCNNNNNNNGC 8 cut(s) 11, 135, 141, 171, 257, 355, 398, 415
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 3 cut(s) 89, 248, 415
NdeI CATATG 1 cut(s) 330
NdeII GATC 2 cut(s) 153, 336
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 2 cut(s) 9, 14
NruI TCGCGA 1 cut(s) 392
NsbI TGCGCA 2 cut(s) 259, 410
NsiI ATGCAT 1 cut(s) 16
NspI RCATGY 1 cut(s) 9
PceI AGGCCT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PctI GAATGC 2 cut(s) 14, 266
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 87
PkrI GCNGC 4 cut(s) 137, 176, 208, 351
RruI TCGCGA 1 cut(s) 392
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 4 cut(s) 136, 175, 207, 350
Sau3AI GATC 2 cut(s) 153, 336
ScrFI CCNGG 3 cut(s) 89, 248, 415
SetI ASST 5 cut(s) 86, 96, 167, 288, 403
SfaNI GCATC 1 cut(s) 336
SgrAI CRCCGGYG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 1 cut(s) 104
SseBI AGGCCT 1 cut(s) 41
SspMI CTAG 4 cut(s) 287, 398, 404, 419
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 3 cut(s) 87, 246, 413
TaqI TCGA 4 cut(s) 18, 78, 161, 344
TasI AATT 1 cut(s) 104
TauI GCSGC 4 cut(s) 138, 177, 209, 352
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TspDTI ATGAA 4 cut(s) 64, 109, 189, 197
TspGWI ACGGA 1 cut(s) 114
XapI RAATTY 1 cut(s) 104
XceI RCATGY 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 4 cut(s) 287, 398, 404, 419
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.