Rmu_ssc0000197.1_g000082

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000197.1
OG0000863
Physical Location & Seq
Reverse (-)
336626 .. 341333
4708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000197.1_g000082.1.cds

Sequence Viewer

Length: 870 bp
atggagagagataagaaaaggcgatgtcgagccaatttgagtgaaaacttgccaacgacagtgacagaatacaccgcgatcttgagtgaatatatggcgatcattagagaagacgtgacggtcatccgagaatacatggcgatcattggagaacacattgtgatcattggagaatacattgcgatcgttagagaatacattgagatcattggagaactgggcacgttcgaggagttaagagagtgcctactaaggtataagcgtgaagctaaagtatgggcgaaggctgccaccgtactcgagattgccaagtgcgacctcatgcagctgagagcacaggcagaggccgagtgcctagaagaaaatgaggctcgtaggcaggtggaaaacatggtaaaccagaatagcatcctccgtgatgcactgaagagaagaatatcgaacaatgaggcacttgacaaggcccgtcaacaaactccaaatgcccaccatcggtctcgccggcaccttcaacaccacccccaaacaggagaggcaacacccggaactcttgcaggttccaaactagggaacacgagcacgggcccacaagtcgtttacatcaactcatccctattcccaagttccctcactgcagcaccattgccaccaccacctcagcctgtacttgacatggcgggcacatccggcattgctacaaccacaaccacctctactgtacctccacctgattttaatacaagattgctcctccaaatgagcgaaactatcgctaccctacaagcgcggattgagaccgtagaaaatagatttggctggcagccgataacgacaagatttgaaggaacgatggaccattcacacctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

289

Amino Acids

32.51

Weight (kDa)

6.27

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000863)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29436 FvH4_4g08647
prunus_persica Prupe.1G244600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0041081 RchiOBHm_Chr7g0227381
rosa_multiflora Rmu_co8094356.1_g000001 Rmu_co8097010.1_g000001 Rmu_co8307317.1_g000001 Rmu_co8331341.1_g000001 Rmu_co8353833.1_g000001 Rmu_co8426601.1_g000001 Rmu_co8464885.1_g000001 Rmu_sc0000161.1_g000045 Rmu_sc0000367.1_g000181 Rmu_sc0000438.1_g000020 Rmu_sc0000637.1_g000008 Rmu_sc0000706.1_g000036 Rmu_sc0000745.1_g000002 Rmu_sc0000964.1_g000007 Rmu_sc0001016.1_g000045 Rmu_sc0001190.1_g000046 Rmu_sc0002014.1_g000040 Rmu_sc0002229.1_g000012 Rmu_sc0002279.1_g000021 Rmu_sc0002279.1_g000049 Rmu_sc0002308.1_g000074 Rmu_sc0002482.1_g000017 Rmu_sc0002809.1_g000002 Rmu_sc0003147.1_g000020 Rmu_sc0003226.1_g000065 Rmu_sc0003246.1_g000004 Rmu_sc0003260.1_g000011 Rmu_sc0003441.1_g000081 Rmu_sc0003895.1_g000019 Rmu_sc0004228.1_g000001 Rmu_sc0004646.1_g000038 Rmu_sc0004688.1_g000018 Rmu_sc0005389.1_g000016 Rmu_sc0005537.1_g000011 Rmu_sc0005954.1_g000010 Rmu_sc0006021.1_g000020 Rmu_sc0006695.1_g000056 Rmu_sc0006822.1_g000010 Rmu_sc0007877.1_g000013 Rmu_sc0008049.1_g000067 Rmu_sc0008691.1_g000004 Rmu_sc0009206.1_g000002 Rmu_sc0011734.1_g000008 Rmu_sc0012136.1_g000012 Rmu_sc0012746.1_g000018 Rmu_sc0013612.1_g000011 Rmu_sc0016149.1_g000005 Rmu_sc0018867.1_g000011 Rmu_sc0020611.1_g000002 Rmu_sc0021291.1_g000004 Rmu_sc0021291.1_g000005 Rmu_sc0022400.1_g000007 Rmu_sc0025427.1_g000001 Rmu_sc0027953.1_g000002 Rmu_sc0033748.1_g000002 Rmu_ssc0000072.1_g000004 Rmu_ssc0000197.1_g000082
rosa_roxburghii Rroxscaffold_4G00282700 Rroxscaffold_5G00374470 Rroxscaffold_7G00183200
rosa_rugosa Rorug02G0187100 Rorug05G0255100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 370
AasI GACNNNNNNGTC 1 cut(s) 119
Acc36I ACCTGC 2 cut(s) 370, 545
AccB1I GGYRCC 1 cut(s) 504
AccII CGCG 2 cut(s) 77, 787
AciI CCGC 3 cut(s) 75, 677, 787
AcuI CTGAAG 1 cut(s) 446
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 3 cut(s) 297, 666, 720
AfiI CCNNNNNNNGG 3 cut(s) 492, 527, 567
AgsI TTSAA 2 cut(s) 512, 842
AjiI CACGTC 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 795, 827
AluBI AGCT 2 cut(s) 269, 328
AluI AGCT 2 cut(s) 269, 328
Alw21I GWGCWC 2 cut(s) 337, 581
Alw26I GTCTC 2 cut(s) 501, 788
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 3 cut(s) 345, 462, 583
ApaI GGGCCC 1 cut(s) 587
ApeKI GCWGC 4 cut(s) 287, 325, 635, 820
AspLEI GCGC 1 cut(s) 787
AspS9I GGNCC 4 cut(s) 463, 583, 584, 853
AsuC2I CCSGG 1 cut(s) 543
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 1 cut(s) 853
BaeGI GKGCMC 3 cut(s) 224, 587, 683
BanI GGYRCC 1 cut(s) 504
BanII GRGCYC 1 cut(s) 587
BauI CACGAG 1 cut(s) 574
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 2 cut(s) 337, 581
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 4 cut(s) 274, 337, 647, 832
BccI CCATC 2 cut(s) 498, 844
BclI TGATCA 1 cut(s) 162
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 2 cut(s) 501, 788
BfaI CTAG 2 cut(s) 356, 566
BfmI CTRYAG 1 cut(s) 633
BfuAI ACCTGC 2 cut(s) 370, 545
BisI GCNGC 4 cut(s) 288, 326, 636, 821
BlsI GCNGC 4 cut(s) 289, 327, 637, 822
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 1 cut(s) 853
BmeT110I CYCGRG 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 4 cut(s) 463, 583, 584, 853
BmiI GGNNCC 3 cut(s) 506, 559, 585
BmrFI CCNGG 1 cut(s) 543
BmrI ACTGGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 409, 417
BmuI ACTGGG 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 543
BsaI GGTCTC 2 cut(s) 501, 788
BsaXI ACNNNNNCTCC 2 cut(s) 706, 736
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 527, 567
Bse118I RCCGGY 1 cut(s) 501
Bse1I ACTGG 1 cut(s) 222
Bse3DI GCAATG 3 cut(s) 177, 641, 690
BseGI GGATG 4 cut(s) 123, 408, 608, 683
BseLI CCNNNNNNNGG 3 cut(s) 492, 527, 567
BseMI GCAATG 3 cut(s) 177, 641, 690
BseMII CTCAG 2 cut(s) 320, 671
BseNI ACTGG 1 cut(s) 222
BseRI GAGGAG 2 cut(s) 245, 740
BseSI GKGCMC 3 cut(s) 224, 587, 683
BseXI GCAGC 4 cut(s) 274, 337, 647, 832
Bsh1236I CGCG 2 cut(s) 77, 787
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 347, 464, 585
BshNI GGYRCC 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 2 cut(s) 337, 581
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 502, 543, 687
BslI CCNNNNNNNGG 3 cut(s) 492, 527, 567
BsmAI GTCTC 2 cut(s) 501, 788
BsnI GGCC 3 cut(s) 347, 464, 585
Bso31I GGTCTC 2 cut(s) 501, 788
BsoBI CYCGRG 1 cut(s) 299
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 5 cut(s) 224, 337, 581, 587, 683
Bsp143I GATC 6 cut(s) 78, 99, 141, 162, 183, 204
BspACI CCGC 3 cut(s) 75, 677, 787
BspANI GGCC 3 cut(s) 347, 464, 585
BspCNI CTCAG 2 cut(s) 321, 670
BspFNI CGCG 2 cut(s) 77, 787
BspLI GGNNCC 3 cut(s) 506, 559, 585
BspMAI CTGCAG 1 cut(s) 637
BspMI ACCTGC 2 cut(s) 370, 545
BspT107I GGYRCC 1 cut(s) 504
BspTNI GGTCTC 2 cut(s) 501, 788
BsrDI GCAATG 3 cut(s) 177, 641, 690
BsrFI RCCGGY 1 cut(s) 501
BsrI ACTGG 1 cut(s) 222
BssAI RCCGGY 1 cut(s) 501
BssMI GATC 6 cut(s) 78, 99, 141, 162, 183, 204
BssSI CACGAG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 574
Bst4CI ACNGT 5 cut(s) 61, 121, 295, 718, 799
Bst6I CTCTTC 1 cut(s) 422
BstC8I GCNNGC 3 cut(s) 503, 679, 818
BstDEI CTNAG 3 cut(s) 251, 329, 657
BstF5I GGATG 4 cut(s) 123, 408, 608, 683
BstFNI CGCG 2 cut(s) 77, 787
BstHHI GCGC 1 cut(s) 787
BstKTI GATC 6 cut(s) 81, 102, 144, 165, 186, 207
BstMAI GTCTC 2 cut(s) 501, 788
BstMBI GATC 6 cut(s) 78, 99, 141, 162, 183, 204
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 287, 687
BstSCI CCNGG 1 cut(s) 541
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 3 cut(s) 224, 587, 683
BstUI CGCG 2 cut(s) 77, 787
BstV1I GCAGC 4 cut(s) 274, 337, 647, 832
BstV2I GAAGAC 1 cut(s) 117
BsuRI GGCC 3 cut(s) 347, 464, 585
BtgZI GCGATG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 4 cut(s) 123, 408, 608, 683
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 3 cut(s) 66, 422, 630
BveI ACCTGC 2 cut(s) 370, 545
Cac8I GCNNGC 3 cut(s) 503, 679, 818
CfoI GCGC 1 cut(s) 787
Cfr10I RCCGGY 1 cut(s) 501
Cfr13I GGNCC 4 cut(s) 463, 583, 584, 853
Csp6I GTAC 3 cut(s) 296, 665, 719
CviAII CATG 4 cut(s) 136, 322, 391, 673
CviQI GTAC 3 cut(s) 296, 665, 719
DdeI CTNAG 3 cut(s) 251, 329, 657
DpnI GATC 6 cut(s) 80, 101, 143, 164, 185, 206
DpnII GATC 6 cut(s) 78, 99, 141, 162, 183, 204
DraIII CACNNNGTG 1 cut(s) 160
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 587
Eco31I GGTCTC 2 cut(s) 501, 788
Eco47I GGWCC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 587
FaeI CATG 4 cut(s) 139, 325, 394, 676
FaiI YATR 8 cut(s) 93, 95, 137, 258, 277, 323, 392, 674
FatI CATG 4 cut(s) 135, 321, 390, 672
FauI CCCGC 1 cut(s) 670
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 4 cut(s) 288, 326, 636, 821
FokI GGATG 4 cut(s) 110, 395, 595, 670
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 4 cut(s) 288, 326, 636, 821
FspBI CTAG 2 cut(s) 356, 566
GlaI GCGC 1 cut(s) 786
GluI GCNGC 4 cut(s) 288, 326, 636, 821
HaeIII GGCC 3 cut(s) 347, 464, 585
HapII CCGG 3 cut(s) 502, 543, 687
HhaI GCGC 1 cut(s) 787
Hin1II CATG 4 cut(s) 139, 325, 394, 676
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HincII GTYRAC 1 cut(s) 470
HindII GTYRAC 1 cut(s) 470
HpaII CCGG 3 cut(s) 502, 543, 687
Hpy166II GTNNAC 3 cut(s) 397, 470, 598
Hpy188I TCNGA 2 cut(s) 128, 869
Hpy188III TCNNGA 2 cut(s) 82, 301
Hpy8I GTNNAC 3 cut(s) 397, 470, 598
HpyAV CCTTC 3 cut(s) 277, 518, 836
HpyCH4III ACNGT 5 cut(s) 61, 121, 295, 718, 799
HpyCH4IV ACGT 2 cut(s) 114, 224
HpyCH4V TGCA 4 cut(s) 325, 422, 554, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 687
HpyF3I CTNAG 3 cut(s) 251, 329, 657
HpySE526I ACGT 2 cut(s) 114, 224
Hsp92II CATG 4 cut(s) 139, 325, 394, 676
HspAI GCGC 1 cut(s) 785
KroI GCCGGC 1 cut(s) 501
KroNI GCCGGC 1 cut(s) 503
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 6 cut(s) 78, 99, 141, 162, 183, 204
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 4 cut(s) 274, 337, 647, 832
LweI GCATC 2 cut(s) 409, 417
MaeI CTAG 2 cut(s) 356, 566
MaeII ACGT 2 cut(s) 114, 224
MaeIII GTNAC 2 cut(s) 61, 115
MalI GATC 6 cut(s) 80, 101, 143, 164, 185, 206
MboI GATC 6 cut(s) 78, 99, 141, 162, 183, 204
MboII GAAGA 4 cut(s) 122, 371, 439, 444
MhlI GDGCHC 5 cut(s) 224, 337, 581, 587, 683
MluCI AATT 1 cut(s) 34
MroNI GCCGGC 1 cut(s) 501
MseI TTAA 2 cut(s) 236, 735
MspA1I CMGCKG 1 cut(s) 328
MspI CCGG 3 cut(s) 502, 543, 687
MspR9I CCNGG 1 cut(s) 543
MvnI CGCG 2 cut(s) 77, 787
MwoI GCNNNNNNNGC 2 cut(s) 287, 687
NaeI GCCGGC 1 cut(s) 503
NciI CCSGG 1 cut(s) 543
NdeII GATC 6 cut(s) 78, 99, 141, 162, 183, 204
NgoMIV GCCGGC 1 cut(s) 501
NlaIII CATG 4 cut(s) 139, 325, 394, 676
NlaIV GGNNCC 3 cut(s) 506, 559, 585
NmeAIII GCCGAG 1 cut(s) 373
NmuCI GTSAC 2 cut(s) 61, 115
PaeR7I CTCGAG 1 cut(s) 299
PaqCI CACCTGC 1 cut(s) 370
PdiI GCCGGC 1 cut(s) 503
PkrI GCNGC 4 cut(s) 289, 327, 637, 822
Ple19I CGATCG 1 cut(s) 186
PspN4I GGNNCC 3 cut(s) 506, 559, 585
PspOMI GGGCCC 1 cut(s) 583
PspPI GGNCC 4 cut(s) 463, 583, 584, 853
PstI CTGCAG 1 cut(s) 637
PvuI CGATCG 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 328
RsaI GTAC 3 cut(s) 297, 666, 720
RsaNI GTAC 3 cut(s) 296, 665, 719
SaqAI TTAA 2 cut(s) 236, 735
SatI GCNGC 4 cut(s) 288, 326, 636, 821
Sau3AI GATC 6 cut(s) 78, 99, 141, 162, 183, 204
Sau96I GGNCC 4 cut(s) 463, 583, 584, 853
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 5 cut(s) 224, 337, 581, 587, 683
SfaNI GCATC 2 cut(s) 409, 417
SfcI CTRYAG 1 cut(s) 633
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 1 cut(s) 853
SlaI CTCGAG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 82, 299
SmoI CTYRAG 2 cut(s) 82, 299
Sse9I AATT 1 cut(s) 34
SsiI CCGC 3 cut(s) 75, 677, 787
SspMI CTAG 2 cut(s) 356, 566
StyD4I CCNGG 1 cut(s) 541
TaaI ACNGT 5 cut(s) 61, 121, 295, 718, 799
TaiI ACGT 2 cut(s) 117, 227
TaqI TCGA 4 cut(s) 28, 228, 300, 440
TaqII GACCGA 1 cut(s) 483
TasI AATT 1 cut(s) 34
TatI WGTACW 1 cut(s) 664
Tru1I TTAA 2 cut(s) 236, 735
Tru9I TTAA 2 cut(s) 236, 735
TscAI CASTG 3 cut(s) 66, 429, 637
TseFI GTSAC 2 cut(s) 61, 115
TseI GCWGC 4 cut(s) 287, 325, 635, 820
Tsp45I GTSAC 2 cut(s) 61, 115
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 3 cut(s) 66, 429, 637
VpaK11BI GGWCC 1 cut(s) 853
XhoI CTCGAG 1 cut(s) 299
XspI CTAG 2 cut(s) 356, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.