FvH4_6g44243

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000412
Physical Location & Seq
Reverse (-)
34096826 .. 34101551
4726 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44243.t8

Sequence Viewer

Length: 2700 bp
ATGACTATGACAGCAAAGATCATGGACACTGGTAATCTTGTTGTGAGCAATAGAGAGCAAGGAAACAACTCAGCTCAAATTGTCTGGCAGAGTTTTGAAAATCCAACCGACACATTTCTTCCTGGCATGAAAATGAGCGCAAAATTAGTACTGAGTTCATGGAAGAGTTACAATGATCCCGCAACAGGAAATTTCACATTTCAGCAAGATCAAGAGGAAGCAAACCACTTTGTTATCTGGAAAAGATCGAAAAGGTACTGGAAGAATGAAGATCATGGCAACTTCATTAGTTCAGATGAGATGGCTTCTGCAATTCTCTACTTGCTATCAAACTTCACCTCTACAGCAGTCCACAATAACTCTGTGCCATATCTCACCTCCTCATTATACACTTCTACAAGGCTGGTTATGAGTTTCTCCGGCCAAATTCAGTATCTATTGTGGGATAGTGAGAAGGTTTGGTCTATGATATGGGCAGATCCTAGAGATAGATGCAGTGTATACAATGCATGTGGAAATTTTGGTAGTTGTAACAGCAAAAATGGTTTGGTTTGCAAGTGTGTGCCTGGTTTTAAGCCTAGTTCACCGGATAATTGGAATCATGGAGATTATTCAGGTGGATGCACTAGGACGTCAACATTATGTGGCAACAATGCTGAGAGTGACACATTCTTGAGCTTAAAGATGATGAAAGTGGGAGATCCAGATTCCCAATTCAATGCCAAAAGTGAAGTGGAATGCAAAGTAGAGTGCCTTAACAACTGTGATTGTCAAGCTTATTTCTATGAAGAGGTGGAGAACAGTAAATCTGGTGGGAGGAGTAGTTCAACATGTTGGATCTGGTCACAAGACGTCACCAATCTTCAGGAGGACTATGAAGGTGGCCGAGATCTACAGGTTCGTGTAGCAGTTTCAGATATAGAATCAACAGCAAGAAGCTGTGGAAGTTGTGGCACAAACCTGATCCCTTATCCCTTGAGCACTGGACCAAGGTGTGGTGATCTTACTTACTACAGTTTTTTATGTAATATCTCAACTGGCCAGTTGAGCTTCGAAGCACCAAGCGGCACCTACCATGTCACAAGCATCAATGCGGACACACAAACATTTGTCATCCAAGCTAATGATGCAGATGGATGTAGAGATGAAAAATTTCTGAAGCTCAACCAGTCTTCTCCATATAATGTGACAGGGATGTGCAAAGCTGATCCGACACGTTTTAGTCCTAACTTGTCATTTAAAGGAGGATATGAAGTTGAAGTTGCTTGGGAGTCACCCTTGGAACCACCTTGTTCGTCATCTACAGACTGCAAGGACTGGGCCCATTCGATATGTGAAGCTACTCTTGATGGGAAGAAAAGGTGTCTTTGCACGGCAGACTCAAAATGGGATGGCAGGAGTTTGAATTGTACTCAAATTGGTCACAGAAAGCAAACTGGTGAGCAAGGGAAGATGACGCTGGCTCTAATCATTGCAGTAACTTGCATAAGTGTAGCTGTTCTGGCAATTCTTTCAAGTACTTTTGCTTATGCTTATCTGTGGAGAAGAAGGCGCATTAAGACAAAAGAAGGCAGGGCATATCTTCAAAAATGTTCAACACTTCATCACTTTTATGACAGTGAGAGAAAAGTCAAGAACTTGATTGAATCAGGCCGATTTAGAGATGATGATACGGAGGGCATTGATGTACCCTCTTTTGATTTGGAAAGCATACTGGTAGCTACAAAATACTTCTCCATTGCAAATAAACTTGGACAAGGAGGATTTGGTCCTGTCTACAAGGGTAAGCTTCCGGGAGGAGAAGAAATCGCTGTAAAAAGGCTCTCAAGTTGTTCAGGCCAAGGCTTAGAAGAATTCAAAAATGAGGTTTTGTTAATTGCCAAACTCCAACATCGAAATCTGGTTCGACTTTTGGGCTATTGCGCTGAGGGAGATGAAAAGATGTTAATCTATGAATACATGGCCAACAAAAGCTTAGACTCTTTCATATTTGATCGGAAAGTATGCGTATCATTGGACTGGAATACACGCTTTAACATCATCCTAGGCATTGCTCGGGGGCTTCTTTATCTTCACCAAGATTCTAGATTAAGGATTATTCATAGAGATCTGAAAACTAGCAACATTCTACTGAGTGAAGAGATGAACCCCAAAATATCAGACTTCGGCTTGGCAAGGATCTTTGGAGGCAATGAAACTTCAGCAAACACCAATAGAGTAGTGGGAACATATGGCTATATGTCTCCGGAGTATGCATTAGATGGATTATTCTCAGTAAAATCTGATGTTTTTAGCTTTGGTGTAGTTGTGATTGAGATCATCACTGGGAAAAGGAACACAGGATTTTATCAGCCTGAAACATCTTTGAGTCTTCTTGGCTATGCATGGCATTTGTGGAAAGAAGAAAAGGCATTAGATTTGCTAGAAAACACACTTTGTCACAGCTGCAACAAGGAGGAGTACTTCAAATGTGTCAATGTTGGACTCTTATGTGTACAAGAAGATCCAGGTGATCGGCCAACCATGTCACAAGTGGTTTTCATGCTCGGAAGTGAAAGTGCAACAATTCCAACCCCTAAACAACCTGCGTTTGTTGTTAGGCGATGCCCTTCTAGTTCTAGCAGGGCTTCTAACTCTTCAAGCAAGCCTGAAACAGTTTCGAACAATGGTTTAACTGTTACCTTAGAAGATGGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

900

Amino Acids

100.18

Weight (kDa)

5.98

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 102 - 197 3.4e-30 D-mannose binding lectin
S_locus_glycop PF00954 241 - 342 2.1e-22 S-locus glycoprotein domain
PAN_2 PF08276 366 - 435 1.5e-10 PAN-like domain
GUB_WAK_bind PF13947 463 - 520 4.5e-06 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 728 - 812 4e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 729 - 812 1e-14 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 635, 855
Acc36I ACCTGC 1 cut(s) 2593
AccB1I GGYRCC 1 cut(s) 1067
AccB7I CCANNNNNTGG 1 cut(s) 995
AccI GTMKAC 2 cut(s) 501, 1776
AccIII TCCGGA 1 cut(s) 2245
AciI CCGC 3 cut(s) 180, 1065, 1094
AclWI GGATC 8 cut(s) 170, 473, 695, 845, 958, 1202, 2186, 2498
AcoI YGGCCR 5 cut(s) 421, 883, 1039, 1962, 2516
AcsI RAATTY 5 cut(s) 190, 426, 517, 1151, 1853
AcuI CTGAAG 3 cut(s) 848, 1178, 2184
AcyI GRCGYC 2 cut(s) 632, 852
AfaI GTAC 7 cut(s) 150, 257, 1411, 1519, 1689, 2462, 2496
AfiI CCNNNNNNNGG 2 cut(s) 185, 995
AflIII ACRYGT 2 cut(s) 830, 1214
AjnI CCWGG 3 cut(s) 121, 565, 2506
AloI GAACNNNNNNTCC 2 cut(s) 808, 840
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 1 cut(s) 2247
AlwI GGATC 8 cut(s) 170, 473, 695, 845, 958, 1202, 2186, 2498
Ama87I CYCGRG 1 cut(s) 2055
Aor13HI TCCGGA 1 cut(s) 2245
AoxI GGCC 8 cut(s) 421, 883, 1039, 1320, 1651, 1837, 1962, 2516
ApaI GGGCCC 1 cut(s) 1324
ApeKI GCWGC 1 cut(s) 2445
ApoI RAATTY 5 cut(s) 190, 426, 517, 1151, 1853
Asp700I GAANNNNTTC 1 cut(s) 1152
AspA2I CCTAGG 1 cut(s) 2044
AspLEI GCGC 3 cut(s) 140, 1554, 1925
AspS9I GGNCC 4 cut(s) 986, 1320, 1321, 1769
AsuC2I CCSGG 1 cut(s) 1794
AsuHPI GGTGA 9 cut(s) 328, 367, 576, 847, 1010, 1266, 1451, 2066, 2522
AsuII TTCGAA 2 cut(s) 1053, 2660
AvaI CYCGRG 1 cut(s) 2055
AvaII GGWCC 2 cut(s) 986, 1769
AvrII CCTAGG 1 cut(s) 2044
BaeGI GKGCMC 1 cut(s) 1324
BalI TGGCCA 2 cut(s) 1041, 1964
BanI GGYRCC 1 cut(s) 1067
BanII GRGCYC 1 cut(s) 1324
BbsI GAAGAC 2 cut(s) 1164, 2363
Bbv12I GWGCWC 1 cut(s) 983
BbvCI CCTCAGC 1 cut(s) 1926
BbvI GCAGC 1 cut(s) 2432
BccI CCATC 6 cut(s) 295, 1127, 1343, 1385, 2255, 2684
BceAI ACGGC 1 cut(s) 1389
BciT130I CCWGG 3 cut(s) 123, 567, 2508
BcnI CCSGG 1 cut(s) 1794
BcoDI GTCTC 1 cut(s) 2247
BfaI CTAG 9 cut(s) 483, 579, 627, 2045, 2085, 2118, 2423, 2613, 2619
BfmI CTRYAG 4 cut(s) 342, 893, 1012, 1302
BfuAI ACCTGC 1 cut(s) 2593
BglII AGATCT 2 cut(s) 889, 2107
BisI GCNGC 2 cut(s) 1066, 2446
BlnI CCTAGG 1 cut(s) 2044
BlsI GCNGC 2 cut(s) 1067, 2447
BmcAI AGTACT 3 cut(s) 150, 1519, 2462
Bme1390I CCNGG 4 cut(s) 123, 567, 1794, 2508
Bme18I GGWCC 2 cut(s) 986, 1769
BmeT110I CYCGRG 1 cut(s) 2055
BmgT120I GGNCC 4 cut(s) 986, 1320, 1321, 1769
BmiI GGNNCC 3 cut(s) 1069, 1284, 1322
BmrFI CCNGG 4 cut(s) 123, 567, 1794, 2508
BmrI ACTGGG 2 cut(s) 1327, 2334
BmsI GCATC 5 cut(s) 482, 611, 1095, 1117, 2594
BmuI ACTGGG 2 cut(s) 1327, 2334
BpiI GAAGAC 2 cut(s) 1164, 2363
Bpu10I CCTNAGC 1 cut(s) 1926
Bpu14I TTCGAA 2 cut(s) 1053, 2660
BpuEI CTTGAG 3 cut(s) 694, 997, 1810
BpuMI CCSGG 1 cut(s) 1794
BsaBI GATNNNNATC 2 cut(s) 1946, 2315
BsaHI GRCGYC 2 cut(s) 632, 852
BsaJI CCNNGG 4 cut(s) 989, 1278, 1840, 2044
BsaWI WCCGGW 2 cut(s) 586, 2245
BsaXI ACNNNNNCTCC 2 cut(s) 808, 838
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 995
Bse3DI GCAATG 4 cut(s) 1470, 1737, 2049, 2197
Bse8I GATNNNNATC 2 cut(s) 1946, 2315
BseAI TCCGGA 1 cut(s) 2245
BseBI CCWGG 3 cut(s) 123, 567, 2508
BseDI CCNNGG 4 cut(s) 989, 1278, 1840, 2044
BseGI GGATG 6 cut(s) 626, 1113, 1142, 1200, 1396, 2040
BseJI GATNNNNATC 2 cut(s) 1946, 2315
BseLI CCNNNNNNNGG 2 cut(s) 185, 995
BseMI GCAATG 4 cut(s) 1470, 1737, 2049, 2197
BseMII CTCAG 6 cut(s) 84, 143, 648, 1917, 2123, 2286
BseRI GAGGAG 4 cut(s) 370, 832, 1812, 2471
BseSI GKGCMC 1 cut(s) 1324
BseXI GCAGC 1 cut(s) 2432
BshFI GGCC 8 cut(s) 423, 885, 1041, 1322, 1653, 1839, 1964, 2518
BshNI GGYRCC 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 983
BsiHKCI CYCGRG 1 cut(s) 2055
BsiSI CCGG 4 cut(s) 420, 587, 1793, 2246
BslI CCNNNNNNNGG 2 cut(s) 185, 995
BsmAI GTCTC 1 cut(s) 2247
BsmI GAATGC 1 cut(s) 743
BsnI GGCC 8 cut(s) 423, 885, 1041, 1322, 1653, 1839, 1964, 2518
BsoBI CYCGRG 1 cut(s) 2055
Bsp119I TTCGAA 2 cut(s) 1053, 2660
Bsp120I GGGCCC 1 cut(s) 1320
Bsp1286I GDGCHC 2 cut(s) 983, 1324
Bsp13I TCCGGA 1 cut(s) 2245
Bsp1407I TGTACA 1 cut(s) 2494
BspACI CCGC 3 cut(s) 180, 1065, 1094
BspANI GGCC 8 cut(s) 423, 885, 1041, 1322, 1653, 1839, 1964, 2518
BspCNI CTCAG 6 cut(s) 83, 144, 649, 1918, 2124, 2285
BspEI TCCGGA 1 cut(s) 2245
BspLI GGNNCC 3 cut(s) 1069, 1284, 1322
BspMI ACCTGC 1 cut(s) 2593
BspPI GGATC 8 cut(s) 170, 473, 695, 845, 958, 1202, 2186, 2498
BspT104I TTCGAA 2 cut(s) 1053, 2660
BspT107I GGYRCC 1 cut(s) 1067
BsrDI GCAATG 4 cut(s) 1470, 1737, 2049, 2197
BsrGI TGTACA 1 cut(s) 2494
BssECI CCNNGG 4 cut(s) 989, 1278, 1840, 2044
BssNAI GTATAC 1 cut(s) 502
BssNI GRCGYC 2 cut(s) 632, 852
BssT1I CCWWGG 4 cut(s) 989, 1278, 1840, 2044
Bst1107I GTATAC 1 cut(s) 502
Bst2UI CCWGG 3 cut(s) 123, 567, 2508
Bst4CI ACNGT 6 cut(s) 764, 803, 1016, 1619, 2656, 2677
Bst6I CTCTTC 4 cut(s) 158, 783, 2133, 2641
BstACI GRCGYC 2 cut(s) 632, 852
BstAUI TGTACA 1 cut(s) 2494
BstBI TTCGAA 2 cut(s) 1053, 2660
BstC8I GCNNGC 2 cut(s) 1461, 2645
BstDEI CTNAG 9 cut(s) 70, 152, 657, 1846, 1926, 1975, 2132, 2272, 2683
BstF5I GGATG 6 cut(s) 626, 1113, 1142, 1200, 1396, 2040
BstHHI GCGC 3 cut(s) 140, 1554, 1925
BstMAI GTCTC 1 cut(s) 2247
BstMWI GCNNNNNNNGC 3 cut(s) 1047, 1127, 1502
BstNI CCWGG 3 cut(s) 123, 567, 2508
BstNSI RCATGY 2 cut(s) 513, 834
BstSCI CCNGG 4 cut(s) 121, 565, 1792, 2506
BstSFI CTRYAG 4 cut(s) 342, 893, 1012, 1302
BstSLI GKGCMC 1 cut(s) 1324
BstV1I GCAGC 1 cut(s) 2432
BstV2I GAAGAC 2 cut(s) 1164, 2363
BstX2I RGATCY 7 cut(s) 478, 700, 837, 889, 2107, 2178, 2503
BstYI RGATCY 7 cut(s) 478, 700, 837, 889, 2107, 2178, 2503
BstZ17I GTATAC 1 cut(s) 502
BsuRI GGCC 8 cut(s) 423, 885, 1041, 1322, 1653, 1839, 1964, 2518
BtgZI GCGATG 1 cut(s) 2617
BtsCI GGATG 6 cut(s) 626, 1113, 1142, 1200, 1396, 2040
BtsI GCAGTG 1 cut(s) 502
BtsIMutI CAGTG 5 cut(s) 27, 502, 981, 1624, 2322
BveI ACCTGC 1 cut(s) 2593
Cac8I GCNNGC 2 cut(s) 1461, 2645
CfoI GCGC 3 cut(s) 140, 1554, 1925
Cfr13I GGNCC 4 cut(s) 986, 1320, 1321, 1769
CseI GACGC 1 cut(s) 1465
Csp6I GTAC 7 cut(s) 149, 256, 1410, 1518, 1688, 2461, 2495
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CviQI GTAC 7 cut(s) 149, 256, 1410, 1518, 1688, 2461, 2495
DdeI CTNAG 9 cut(s) 70, 152, 657, 1846, 1926, 1975, 2132, 2272, 2683
DraI TTTAAA 1 cut(s) 1240
EaeI YGGCCR 5 cut(s) 421, 883, 1039, 1962, 2516
Eam1104I CTCTTC 4 cut(s) 158, 783, 2133, 2641
EarI CTCTTC 4 cut(s) 158, 783, 2133, 2641
Eco130I CCWWGG 4 cut(s) 989, 1278, 1840, 2044
Eco24I GRGCYC 1 cut(s) 1324
Eco47I GGWCC 2 cut(s) 986, 1769
Eco57I CTGAAG 3 cut(s) 848, 1178, 2184
Eco88I CYCGRG 1 cut(s) 2055
EcoRI GAATTC 1 cut(s) 1853
EcoRII CCWGG 3 cut(s) 121, 565, 2506
EcoT14I CCWWGG 4 cut(s) 989, 1278, 1840, 2044
EcoT22I ATGCAT 3 cut(s) 511, 2257, 2386
EcoT38I GRGCYC 1 cut(s) 1324
ErhI CCWWGG 4 cut(s) 989, 1278, 1840, 2044
FalI AAGNNNNNCTT 2 cut(s) 1329, 1361
FauI CCCGC 1 cut(s) 187
FauNDI CATATG 1 cut(s) 2230
FblI GTMKAC 2 cut(s) 501, 1776
Fnu4HI GCNGC 2 cut(s) 1066, 2446
FokI GGATG 6 cut(s) 633, 1100, 1149, 1207, 1403, 2027
FriOI GRGCYC 1 cut(s) 1324
Fsp4HI GCNGC 2 cut(s) 1066, 2446
FspBI CTAG 9 cut(s) 483, 579, 627, 2045, 2085, 2118, 2423, 2613, 2619
GlaI GCGC 3 cut(s) 139, 1553, 1924
GluI GCNGC 2 cut(s) 1066, 2446
HaeIII GGCC 8 cut(s) 423, 885, 1041, 1322, 1653, 1839, 1964, 2518
HapII CCGG 4 cut(s) 420, 587, 1793, 2246
HgaI GACGC 1 cut(s) 1465
HhaI GCGC 3 cut(s) 140, 1554, 1925
Hin1I GRCGYC 2 cut(s) 632, 852
Hin6I GCGC 3 cut(s) 138, 1552, 1923
HinP1I GCGC 3 cut(s) 138, 1552, 1923
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HindIII AAGCTT 3 cut(s) 774, 1787, 1972
HpaII CCGG 4 cut(s) 420, 587, 1793, 2246
HphI GGTGA 9 cut(s) 328, 367, 576, 847, 1010, 1266, 1451, 2066, 2522
Hpy166II GTNNAC 6 cut(s) 352, 502, 584, 636, 1777, 2495
Hpy188I TCNGA 9 cut(s) 295, 916, 1158, 1212, 1998, 2112, 2161, 2284, 2549
Hpy188III TCNNGA 9 cut(s) 212, 238, 673, 704, 866, 1346, 1633, 2085, 2246
Hpy8I GTNNAC 6 cut(s) 352, 502, 584, 636, 1777, 2495
HpyAV CCTTC 5 cut(s) 448, 872, 1542, 1562, 2619
HpyCH4III ACNGT 6 cut(s) 764, 803, 1016, 1619, 2656, 2677
HpyCH4IV ACGT 3 cut(s) 632, 852, 1216
HpyF10VI GCNNNNNNNGC 3 cut(s) 1047, 1127, 1502
HpyF3I CTNAG 9 cut(s) 70, 152, 657, 1846, 1926, 1975, 2132, 2272, 2683
HpySE526I ACGT 3 cut(s) 632, 852, 1216
Hsp92I GRCGYC 2 cut(s) 632, 852
HspAI GCGC 3 cut(s) 138, 1552, 1923
Kpn2I TCCGGA 1 cut(s) 2245
Lsp1109I GCAGC 1 cut(s) 2432
LweI GCATC 5 cut(s) 482, 611, 1095, 1117, 2594
MaeI CTAG 9 cut(s) 483, 579, 627, 2045, 2085, 2118, 2423, 2613, 2619
MaeII ACGT 3 cut(s) 632, 852, 1216
MflI RGATCY 7 cut(s) 478, 700, 837, 889, 2107, 2178, 2503
MhlI GDGCHC 2 cut(s) 983, 1324
MlsI TGGCCA 2 cut(s) 1041, 1964
MluNI TGGCCA 2 cut(s) 1041, 1964
MlyI GAGTC 5 cut(s) 1280, 1373, 1973, 2377, 2478
MmeI TCCRAC 6 cut(s) 128, 815, 1235, 1912, 2461, 2594
Mox20I TGGCCA 2 cut(s) 1041, 1964
Mph1103I ATGCAT 3 cut(s) 511, 2257, 2386
MroI TCCGGA 1 cut(s) 2245
MroXI GAANNNNTTC 1 cut(s) 1152
MscI TGGCCA 2 cut(s) 1041, 1964
MslI CAYNNNNRTG 2 cut(s) 131, 1611
Msp20I TGGCCA 2 cut(s) 1041, 1964
MspA1I CMGCKG 1 cut(s) 2445
MspI CCGG 4 cut(s) 420, 587, 1793, 2246
MspR9I CCNGG 4 cut(s) 123, 567, 1794, 2508
Mva1269I GAATGC 1 cut(s) 743
MvaI CCWGG 3 cut(s) 123, 567, 2508
MwoI GCNNNNNNNGC 3 cut(s) 1047, 1127, 1502
NciI CCSGG 1 cut(s) 1794
NdeI CATATG 1 cut(s) 2230
NlaIV GGNNCC 3 cut(s) 1069, 1284, 1322
NmeAIII GCCGAG 1 cut(s) 911
NmuCI GTSAC 9 cut(s) 662, 843, 853, 1078, 1186, 1272, 1421, 2438, 2526
NsiI ATGCAT 3 cut(s) 511, 2257, 2386
NspI RCATGY 2 cut(s) 513, 834
NspV TTCGAA 2 cut(s) 1053, 2660
PciI ACATGT 1 cut(s) 830
PctI GAATGC 1 cut(s) 743
PdmI GAANNNNTTC 1 cut(s) 1152
PfeI GAWTC 5 cut(s) 598, 707, 923, 1646, 2081
PflMI CCANNNNNTGG 1 cut(s) 995
PfoI TCCNGGA 1 cut(s) 1792
PkrI GCNGC 2 cut(s) 1067, 2447
PleI GAGTC 5 cut(s) 1279, 1373, 1973, 2376, 2478
PpsI GAGTC 5 cut(s) 1279, 1373, 1973, 2376, 2478
PscI ACATGT 1 cut(s) 830
Psp6I CCWGG 3 cut(s) 121, 565, 2506
PspGI CCWGG 3 cut(s) 121, 565, 2506
PspN4I GGNNCC 3 cut(s) 1069, 1284, 1322
PspOMI GGGCCC 1 cut(s) 1320
PspPI GGNCC 4 cut(s) 986, 1320, 1321, 1769
PsuI RGATCY 7 cut(s) 478, 700, 837, 889, 2107, 2178, 2503
PvuII CAGCTG 1 cut(s) 2445
RsaI GTAC 7 cut(s) 150, 257, 1411, 1519, 1689, 2462, 2496
RsaNI GTAC 7 cut(s) 149, 256, 1410, 1518, 1688, 2461, 2495
RseI CAYNNNNRTG 2 cut(s) 131, 1611
SatI GCNGC 2 cut(s) 1066, 2446
Sau96I GGNCC 4 cut(s) 986, 1320, 1321, 1769
ScaI AGTACT 3 cut(s) 150, 1519, 2462
SchI GAGTC 5 cut(s) 1280, 1373, 1973, 2377, 2478
ScrFI CCNGG 4 cut(s) 123, 567, 1794, 2508
SduI GDGCHC 2 cut(s) 983, 1324
SfaNI GCATC 5 cut(s) 482, 611, 1095, 1117, 2594
SfcI CTRYAG 4 cut(s) 342, 893, 1012, 1302
SfuI TTCGAA 2 cut(s) 1053, 2660
SinI GGWCC 2 cut(s) 986, 1769
SmiMI CAYNNNNRTG 2 cut(s) 131, 1611
SmlI CTYRAG 3 cut(s) 673, 976, 1825
SmoI CTYRAG 3 cut(s) 673, 976, 1825
SsiI CCGC 3 cut(s) 180, 1065, 1094
SspMI CTAG 9 cut(s) 483, 579, 627, 2045, 2085, 2118, 2423, 2613, 2619
StyD4I CCNGG 4 cut(s) 121, 565, 1792, 2506
StyI CCWWGG 4 cut(s) 989, 1278, 1840, 2044
TaaI ACNGT 6 cut(s) 764, 803, 1016, 1619, 2656, 2677
TaiI ACGT 3 cut(s) 635, 855, 1219
TaqI TCGA 7 cut(s) 248, 1053, 1328, 1894, 1906, 2660, 2695
TatI WGTACW 5 cut(s) 148, 1409, 1517, 2460, 2494
TauI GCSGC 1 cut(s) 1068
TfiI GAWTC 5 cut(s) 598, 707, 923, 1646, 2081
TscAI CASTG 5 cut(s) 34, 502, 988, 1624, 2329
TseFI GTSAC 9 cut(s) 662, 843, 853, 1078, 1186, 1272, 1421, 2438, 2526
TseI GCWGC 1 cut(s) 2445
Tsp45I GTSAC 9 cut(s) 662, 843, 853, 1078, 1186, 1272, 1421, 2438, 2526
TspGWI ACGGA 1 cut(s) 1688
TspRI CASTG 5 cut(s) 34, 502, 988, 1624, 2329
Van91I CCANNNNNTGG 1 cut(s) 995
VpaK11BI GGWCC 2 cut(s) 986, 1769
XapI RAATTY 5 cut(s) 190, 426, 517, 1151, 1853
XbaI TCTAGA 1 cut(s) 2084
XceI RCATGY 2 cut(s) 513, 834
XcmI CCANNNNNNNNNTGG 3 cut(s) 730, 2218, 2530
XmaJI CCTAGG 1 cut(s) 2044
XmiI GTMKAC 2 cut(s) 501, 1776
XmnI GAANNNNTTC 1 cut(s) 1152
XspI CTAG 9 cut(s) 483, 579, 627, 2045, 2085, 2118, 2423, 2613, 2619
ZraI GACGTC 2 cut(s) 633, 853
ZrmI AGTACT 3 cut(s) 150, 1519, 2462
Zsp2I ATGCAT 3 cut(s) 511, 2257, 2386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.