Rw4G006230

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000412
Physical Location & Seq
Reverse (-)
12718923 .. 12722947
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006230.1

Sequence Viewer

Length: 3003 bp
ATGGTATCTTACGTCTTTTTCTTGTATGTAGTACTATTGCTGTGCGCTTATGACAACCTATTTTGTTCTGCTAGAGATACACTAACATCTGACGATCCAGTTATCGATGATGGATCAGAAGCTCTTGTTTCAGCAGGAGGTGAGTTTGAACTGGGTTTCTTCTCTACTACTACGGGGAAAAGATATGTGGGCATATGGTATCATAAGGTCACTCCAAGAACAGTTGTATGGGTTGCCAACAGAGCTGAACCTCTTCCTGCAGATTCCACTGGAGTTCTTACAATTGAAGAAGGGAATCTCCGCGTGTTTGATCATGTTACTGGGAAGCGTTATTGGTCAACAGAGATTGGAAAATCTTTTAACAAGACAGTGAAGATAATGGATTCAGGGAACCTGGTGTTAAGGGATACTGGTGAGTTGACAGCGGATCCTTTGTGGCAGAGCTTTCAAAATCCAACTGATACATTCATTCCTGGGATGCGAATGGATAGTAACTTGAAGTTGACTTCATGGAGCGACGAAGATGACCGACGAACTGGGAACTACACCTTCGAACTAGATCAAGAAGGGAAGTCAGAGAACCAGTTTACCATCTTAAATAGTTCGTTTCGTTACTGGAAAAGTGGAGAGCCAGGTAAATATTTACGCTCAGATGAGATGCGTCCTGAGGTAGCTTACTTGTTATCAAATTTCAGCAAGAATTCTTCTCTTAATACAACTCAGCCTCAATTTGATTACAATTATACAAGATTGGTGATGAATTCTACTGGAGTGCTCAATGGTTTGACCTGGAATGACACCATTAAGCAGTGGGTTGTGTTTTTCTCTGCGCCAAAAGATCAATGCAGTGTGATTAATGCTTGTGGAAACTTTGGCAGCTGCAACATTAATAATACCCCGCTGGTGTGCAAATGTTTGCCAGGTTTCAACCCCCAGTTTTTAGAGAAATGGAAATATGGAGATTTTTCAGATGGATGCAAAAGACAGTCGTCCATATGTGGCATGAAAGATACCTTCTTAAGCATAAAGGTGAACAAAGTGGGAATCTGGGAGTCATCGACCAATGTTACCAGTGATACAGAATGCGAGAAAGCGTGCCGTGGTAATTGCAAGTGCCAGGCTTACTCATCTGATTCATCTGTAAAGGCCACTGTTCGAAGGGATGATCTGAATATCTGTTATCAATGGTCCACGGATCTAAATGATCTTGTAGAGGAGTCTGGTGACAATGGCAAGATTCTCTATGCTCGAGTTCCCATGCCTGATTTAGCATCAACTACAAGAGACTGTAAACCTTGTGGCACAACTACAATTCCTTATCCTCTGAGCACTACTGGAACAGATTGTGGCGACCCCATGTACAAAAGTTTCGCGTGCAATGCCTCCATTAATGAATTTAGCTTCCTTGGACCAGATGGCAAATCCTTTAGAGTTATAAATATCAGCCGAAGTACACAACGATTCATTATCAAATCCAAAAATATGAATTATTGTGATTCTAGAAACAGAGTGCTGAACATTTCCTTCCCGTTTAAGGTAATCAGTTGGTGTAGTTCTGACCAGATGAGGAAATTTATTTCTGGCATAGATTCATCCAGCAGTCCTTCAGTAATAGAGATGGGTTGGGAGCCACCATCGGAAGCAAACTGCACTATCATAGACTGCAGGGATTGGTCATATTCAACTTGCAAAACAGTAAGAGGGGGAGAGAAAAAATGCCTTTGCAATACAAATTTTCAATGGAACGGCATTGACTTAAATTGTACTCAAGAAGGAAATCTACAAGAGCTATCAAATAAGAGTTCAACGAAAAAATTGTTATTGTCTCTGGTTGTCGTGGCAGTCATAAGTGGGATTTTTCTGGCATGCATCATTGGTTTTTGGATATGGAGAAGAAAGATTACAAACAGACGAGATCAAGTAAGTCGTCCGCAGCTCGATAGTGAAAGACGAGTCAAAGAACTCATAGACACGAGTGACTTTAATGAAGAAGCTGACAAAGGCATAGATGTACCTTTTTTTGATTTGCAAAGCATACTAGACGCTACAGATAATTTCTCAATTGGAAACAAGCTTGGACAAGGGGGGTATGGGCCTGTTTACAAGGGGATCTTTCCTGGAGGTCAAGAAATTGCTGTAAAGAGGCTATCAAGAGTTTCGGGACAAGGCTTACAGGAGTTTAAGAATGAGGTGGTGCTAATCGCCAAACTTCAACATCGTAATCTTGTAAGACTCAGAGGATATTGCATGAAAGGAGAAGAAAAGATTTTACTCTACGAGTACATGCCTAACAAAAGCTTAGATTCCTTCATATTTGATCATACACAAAGCATGTTTCTCAATTGGGAGATGCGGTTTAACATCATTTTGGGAATTGCTCGAGGACTTCTTTATCTTCATCAGGATTCCAGGTTGAGGATCATTCATAGAGATTTGAAAACCAGCAATGTTCTTTTGGATGAGGAGATGAACCCCAAAATATCTGACTTCGGCTTGGCCAGGATTGTTGGAGGCAAAGAAACCGAGGCAAACACAAATACAGTAGTAGGAACCTATGGTTACATGTCGCCGGAATATGCATTGGATGGAAATTTCTCTGTCAAATCAGATGTCTTTAGTTTCGGTGTGGTTCTTCTAGAGATAATCAGCGGAAAAAAGAATGCAGGATTTTATCAGTCCAAACAAACTTTCAGTCTCCTTAGTTATACATGGGGACTCTGGACAGAAAACAAGGTGTTGGATTTAATGGACAACAATTTGGAAGAAAGTTGCAACAGAAGTGAATTTATAAAGTGTGTAAATGTTGGGCTCTTATGCGTACAAGAAGATCCAGTTGATCGCCCTACAATGTCAAATGTTGTTACCTTGCTTGACAGTGAAACTGCAATCCTACCAACTCCTAAACAACCAGCGTTTTTTATACGGAGAGGCAACTCTAGCACAGCTTCCTCTTCTACTAAACCAGAAACATACTCTGAAATAACTACTCTGGAAGGAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1000

Amino Acids

112.57

Weight (kDa)

5.71

Isoelectric Point (pI)

42.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 167 2.2e-23 D-mannose binding lectin
S_locus_glycop PF00954 217 - 312 7.5e-21 S-locus glycoprotein domain
PAN_2 PF08276 333 - 403 6.5e-07 PAN-like domain
Pkinase PF00069 687 - 954 1.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 688 - 956 1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1436, 2789
AccII CGCG 2 cut(s) 303, 1373
AciI CCGC 6 cut(s) 301, 425, 899, 1931, 2353, 2649
AclWI GGATC 8 cut(s) 89, 121, 422, 435, 1203, 2117, 2426, 2822
AcoI YGGCCR 1 cut(s) 2496
AcsI RAATTY 8 cut(s) 688, 700, 760, 1394, 1571, 1732, 2590, 2783
AcuI CTGAAG 1 cut(s) 1590
AfaI GTAC 7 cut(s) 33, 1361, 1453, 1765, 2013, 2282, 2820
AfiI CCNNNNNNNGG 2 cut(s) 1534, 2415
AflII CTTAAG 1 cut(s) 1018
AflIII ACRYGT 1 cut(s) 2562
AjnI CCWGG 9 cut(s) 393, 472, 631, 788, 919, 1116, 2116, 2408, 2498
AjuI GAANNNNNNNTTGG 2 cut(s) 2672, 2704
Alw21I GWGCWC 2 cut(s) 777, 1331
Alw26I GTCTC 3 cut(s) 1278, 1830, 2699
AlwI GGATC 8 cut(s) 89, 121, 422, 435, 1203, 2117, 2426, 2822
Ama87I CYCGRG 2 cut(s) 1248, 2379
AoxI GGCC 3 cut(s) 1146, 2093, 2496
ApeKI GCWGC 3 cut(s) 876, 879, 1933
ApoI RAATTY 8 cut(s) 688, 700, 760, 1394, 1571, 1732, 2590, 2783
ArsI GACNNNNNNTTYG 4 cut(s) 2595, 2627, 2677, 2709
AseI ATTAAT 3 cut(s) 855, 888, 1389
Asp700I GAANNNNTTC 1 cut(s) 252
AspLEI GCGC 2 cut(s) 47, 832
AspS9I GGNCC 3 cut(s) 1188, 1409, 2093
AsuHPI GGTGA 5 cut(s) 152, 425, 766, 1042, 1235
AsuII TTCGAA 2 cut(s) 552, 1156
AvaI CYCGRG 2 cut(s) 1248, 2379
AvaII GGWCC 2 cut(s) 1188, 1409
AxyI CCTNAGG 1 cut(s) 666
BalI TGGCCA 1 cut(s) 2498
BamHI GGATCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 2811
BarI GAAGNNNNNNTAC 4 cut(s) 1764, 1796, 1885, 1917
BauI CACGAG 1 cut(s) 1972
Bbv12I GWGCWC 2 cut(s) 777, 1331
BbvI GCAGC 3 cut(s) 866, 888, 1945
BccI CCATC 7 cut(s) 104, 599, 965, 1409, 1612, 1642, 2579
BceAI ACGGC 2 cut(s) 1083, 1762
BciT130I CCWGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
BciVI GTATCC 1 cut(s) 400
BclI TGATCA 2 cut(s) 310, 2317
BcoDI GTCTC 3 cut(s) 1278, 1830, 2699
BfaI CTAG 6 cut(s) 72, 557, 1500, 2039, 2636, 2937
BfmI CTRYAG 3 cut(s) 258, 1663, 2046
BfrI CTTAAG 1 cut(s) 1018
BfuI GTATCC 1 cut(s) 400
BisI GCNGC 3 cut(s) 877, 880, 1934
BlsI GCNGC 3 cut(s) 878, 881, 1935
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
Bme18I GGWCC 2 cut(s) 1188, 1409
BmeT110I CYCGRG 2 cut(s) 1248, 2379
BmgT120I GGNCC 3 cut(s) 1188, 1409, 2093
BmiI GGNNCC 4 cut(s) 392, 429, 1629, 2551
BmrFI CCNGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
BmrI ACTGGG 4 cut(s) 161, 330, 546, 928
BmsI GCATC 6 cut(s) 468, 648, 965, 1280, 1878, 2340
BmuI ACTGGG 4 cut(s) 161, 330, 546, 928
BoxI GACNNNNGTC 1 cut(s) 988
BpmI CTGGAG 3 cut(s) 291, 789, 2139
Bpu14I TTCGAA 2 cut(s) 552, 1156
BpuEI CTTGAG 1 cut(s) 1752
Bsa29I ATCGAT 1 cut(s) 105
BsaJI CCNNGG 5 cut(s) 473, 1099, 1191, 1405, 2523
BsaXI ACNNNNNCTCC 2 cut(s) 2339, 2369
Bsc4I CCNNNNNNNGG 2 cut(s) 1534, 2415
Bse21I CCTNAGG 1 cut(s) 666
Bse3DI GCAATG 2 cut(s) 1384, 2452
BseBI CCWGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 5 cut(s) 473, 1099, 1191, 1405, 2523
BseGI GGATG 6 cut(s) 483, 980, 1168, 1592, 2464, 2590
BseLI CCNNNNNNNGG 2 cut(s) 1534, 2415
BseMI GCAATG 2 cut(s) 1384, 2452
BseMII CTCAG 5 cut(s) 657, 663, 734, 1316, 2248
BseRI GAGGAG 2 cut(s) 1229, 2477
BseXI GCAGC 3 cut(s) 866, 888, 1945
BsgI GTGCAG 1 cut(s) 1633
Bsh1236I CGCG 2 cut(s) 303, 1373
BshFI GGCC 3 cut(s) 1148, 2095, 2498
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 777, 1331
BsiHKCI CYCGRG 2 cut(s) 1248, 2379
BsiSI CCGG 1 cut(s) 2570
BslFI GGGAC 2 cut(s) 2175, 2727
BslI CCNNNNNNNGG 2 cut(s) 1534, 2415
BsmAI GTCTC 3 cut(s) 1278, 1830, 2699
BsmFI GGGAC 2 cut(s) 2175, 2727
BsmI GAATGC 2 cut(s) 1088, 2665
BsnI GGCC 3 cut(s) 1148, 2095, 2498
BsoBI CYCGRG 2 cut(s) 1248, 2379
Bsp119I TTCGAA 2 cut(s) 552, 1156
Bsp1286I GDGCHC 3 cut(s) 777, 1331, 2811
Bsp1407I TGTACA 1 cut(s) 1359
BspACI CCGC 6 cut(s) 301, 425, 899, 1931, 2353, 2649
BspANI GGCC 3 cut(s) 1148, 2095, 2498
BspCNI CTCAG 5 cut(s) 658, 662, 733, 1317, 2247
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 2 cut(s) 303, 1373
BspLI GGNNCC 4 cut(s) 392, 429, 1629, 2551
BspMAI CTGCAG 2 cut(s) 262, 1667
BspPI GGATC 8 cut(s) 89, 121, 422, 435, 1203, 2117, 2426, 2822
BspT104I TTCGAA 2 cut(s) 552, 1156
BspTI CTTAAG 1 cut(s) 1018
BsrDI GCAATG 2 cut(s) 1384, 2452
BsrGI TGTACA 1 cut(s) 1359
BssECI CCNNGG 5 cut(s) 473, 1099, 1191, 1405, 2523
BssSI CACGAG 1 cut(s) 1972
BssT1I CCWWGG 1 cut(s) 1405
Bst2BI CACGAG 1 cut(s) 1972
Bst2UI CCWGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
Bst4CI ACNGT 8 cut(s) 223, 370, 987, 1153, 1289, 1696, 2542, 2876
Bst6I CTCTTC 2 cut(s) 258, 2956
BstAFI CTTAAG 1 cut(s) 1018
BstAUI TGTACA 1 cut(s) 1359
BstBI TTCGAA 2 cut(s) 552, 1156
BstC8I GCNNGC 3 cut(s) 1096, 1375, 1867
BstDEI CTNAG 7 cut(s) 649, 666, 720, 1325, 2234, 2299, 2699
BstDSI CCRYGG 2 cut(s) 1099, 1191
BstF5I GGATG 6 cut(s) 483, 980, 1168, 1592, 2464, 2590
BstFNI CGCG 2 cut(s) 303, 1373
BstHHI GCGC 2 cut(s) 47, 832
BstMAI GTCTC 3 cut(s) 1278, 1830, 2699
BstMWI GCNNNNNNNGC 3 cut(s) 242, 1379, 2937
BstNI CCWGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
BstNSI RCATGY 4 cut(s) 1869, 2287, 2335, 2566
BstPAI GACNNNNGTC 1 cut(s) 988
BstSCI CCNGG 9 cut(s) 393, 472, 631, 788, 919, 1116, 2116, 2408, 2498
BstSFI CTRYAG 3 cut(s) 258, 1663, 2046
BstUI CGCG 2 cut(s) 303, 1373
BstV1I GCAGC 3 cut(s) 866, 888, 1945
BstX2I RGATCY 4 cut(s) 427, 1195, 2109, 2827
BstYI RGATCY 4 cut(s) 427, 1195, 2109, 2827
Bsu15I ATCGAT 1 cut(s) 105
Bsu36I CCTNAGG 1 cut(s) 666
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 3 cut(s) 1148, 2095, 2498
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 2 cut(s) 1099, 1191
BtsCI GGATG 6 cut(s) 483, 980, 1168, 1592, 2464, 2590
BtsI GCAGTG 2 cut(s) 815, 853
BtsIMutI CAGTG 7 cut(s) 267, 375, 815, 853, 1078, 1149, 2881
Cac8I GCNNGC 3 cut(s) 1096, 1375, 1867
CfoI GCGC 2 cut(s) 47, 832
Cfr13I GGNCC 3 cut(s) 1188, 1409, 2093
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 2 cut(s) 650, 2051
CsiI ACCWGGT 1 cut(s) 393
Csp6I GTAC 7 cut(s) 32, 1360, 1452, 1764, 2012, 2281, 2819
CviQI GTAC 7 cut(s) 32, 1360, 1452, 1764, 2012, 2281, 2819
DdeI CTNAG 7 cut(s) 649, 666, 720, 1325, 2234, 2299, 2699
EaeI YGGCCR 1 cut(s) 2496
Eam1104I CTCTTC 2 cut(s) 258, 2956
EarI CTCTTC 2 cut(s) 258, 2956
Eco130I CCWWGG 1 cut(s) 1405
Eco24I GRGCYC 1 cut(s) 2811
Eco47I GGWCC 2 cut(s) 1188, 1409
Eco57I CTGAAG 1 cut(s) 1590
Eco81I CCTNAGG 1 cut(s) 666
Eco88I CYCGRG 2 cut(s) 1248, 2379
EcoRI GAATTC 2 cut(s) 700, 760
EcoRII CCWGG 9 cut(s) 393, 472, 631, 788, 919, 1116, 2116, 2408, 2498
EcoT14I CCWWGG 1 cut(s) 1405
EcoT22I ATGCAT 2 cut(s) 1871, 2581
EcoT38I GRGCYC 1 cut(s) 2811
ErhI CCWWGG 1 cut(s) 1405
FalI AAGNNNNNCTT 2 cut(s) 2096, 2128
FaqI GGGAC 2 cut(s) 2175, 2727
FauI CCCGC 1 cut(s) 906
FauNDI CATATG 2 cut(s) 194, 995
FbaI TGATCA 2 cut(s) 310, 2317
Fnu4HI GCNGC 3 cut(s) 877, 880, 1934
FokI GGATG 6 cut(s) 490, 987, 1175, 1579, 2471, 2597
FriOI GRGCYC 1 cut(s) 2811
Fsp4HI GCNGC 3 cut(s) 877, 880, 1934
FspBI CTAG 6 cut(s) 72, 557, 1500, 2039, 2636, 2937
GlaI GCGC 2 cut(s) 46, 831
GluI GCNGC 3 cut(s) 877, 880, 1934
GsuI CTGGAG 3 cut(s) 291, 789, 2139
HaeIII GGCC 3 cut(s) 1148, 2095, 2498
HapII CCGG 1 cut(s) 2570
HgaI GACGC 2 cut(s) 650, 2051
HhaI GCGC 2 cut(s) 47, 832
Hin6I GCGC 2 cut(s) 45, 830
HinP1I GCGC 2 cut(s) 45, 830
HincII GTYRAC 3 cut(s) 339, 420, 504
HindII GTYRAC 3 cut(s) 339, 420, 504
HindIII AAGCTT 2 cut(s) 2072, 2296
HpaII CCGG 1 cut(s) 2570
HphI GGTGA 5 cut(s) 152, 425, 766, 1042, 1235
Hpy166II GTNNAC 9 cut(s) 339, 420, 504, 588, 1033, 1191, 1292, 1454, 2101
Hpy8I GTNNAC 9 cut(s) 339, 420, 504, 588, 1033, 1191, 1292, 1454, 2101
Hpy99I CGWCG 2 cut(s) 521, 534
HpyCH4III ACNGT 8 cut(s) 223, 370, 987, 1153, 1289, 1696, 2542, 2876
HpyCH4IV ACGT 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 1379, 2937
HpyF3I CTNAG 7 cut(s) 649, 666, 720, 1325, 2234, 2299, 2699
HpySE526I ACGT 1 cut(s) 12
HspAI GCGC 2 cut(s) 45, 830
Ksp22I TGATCA 2 cut(s) 310, 2317
LmnI GCTCC 2 cut(s) 513, 1627
Lsp1109I GCAGC 3 cut(s) 866, 888, 1945
LweI GCATC 6 cut(s) 468, 648, 965, 1280, 1878, 2340
MabI ACCWGGT 1 cut(s) 393
MaeI CTAG 6 cut(s) 72, 557, 1500, 2039, 2636, 2937
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 9 cut(s) 208, 316, 491, 611, 1066, 1223, 1976, 2558, 2860
MfeI CAATTG 3 cut(s) 282, 2061, 2341
MflI RGATCY 4 cut(s) 427, 1195, 2109, 2827
MhlI GDGCHC 3 cut(s) 777, 1331, 2811
MlsI TGGCCA 1 cut(s) 2498
MluNI TGGCCA 1 cut(s) 2498
MlyI GAGTC 5 cut(s) 1061, 1226, 1962, 2226, 2709
MmeI TCCRAC 3 cut(s) 479, 2488, 2718
Mox20I TGGCCA 1 cut(s) 2498
Mph1103I ATGCAT 2 cut(s) 1871, 2581
MroXI GAANNNNTTC 1 cut(s) 252
MscI TGGCCA 1 cut(s) 2498
MslI CAYNNNNRTG 1 cut(s) 1028
Msp20I TGGCCA 1 cut(s) 2498
MspA1I CMGCKG 4 cut(s) 425, 879, 901, 2649
MspCI CTTAAG 1 cut(s) 1018
MspI CCGG 1 cut(s) 2570
MspR9I CCNGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
MunI CAATTG 3 cut(s) 282, 2061, 2341
Mva1269I GAATGC 2 cut(s) 1088, 2665
MvaI CCWGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
MvnI CGCG 2 cut(s) 303, 1373
MwoI GCNNNNNNNGC 3 cut(s) 242, 1379, 2937
NdeI CATATG 2 cut(s) 194, 995
NlaIV GGNNCC 4 cut(s) 392, 429, 1629, 2551
NmuCI GTSAC 3 cut(s) 208, 1223, 1976
NsiI ATGCAT 2 cut(s) 1871, 2581
NspI RCATGY 4 cut(s) 1869, 2287, 2335, 2566
NspV TTCGAA 2 cut(s) 552, 1156
PaeI GCATGC 1 cut(s) 1869
PaeR7I CTCGAG 2 cut(s) 1248, 2379
PciI ACATGT 1 cut(s) 2562
PctI GAATGC 2 cut(s) 1088, 2665
PdmI GAANNNNTTC 1 cut(s) 252
PfoI TCCNGGA 1 cut(s) 2116
PkrI GCNGC 3 cut(s) 878, 881, 1935
PleI GAGTC 5 cut(s) 1060, 1225, 1961, 2226, 2709
PpsI GAGTC 5 cut(s) 1060, 1225, 1961, 2226, 2709
PscI ACATGT 1 cut(s) 2562
PshAI GACNNNNGTC 1 cut(s) 988
PshBI ATTAAT 3 cut(s) 855, 888, 1389
PsiI TTATAA 2 cut(s) 1436, 2789
Psp6I CCWGG 9 cut(s) 393, 472, 631, 788, 919, 1116, 2116, 2408, 2498
PspGI CCWGG 9 cut(s) 393, 472, 631, 788, 919, 1116, 2116, 2408, 2498
PspN4I GGNNCC 4 cut(s) 392, 429, 1629, 2551
PspPI GGNCC 3 cut(s) 1188, 1409, 2093
PspXI VCTCGAGB 2 cut(s) 1248, 2379
PstI CTGCAG 2 cut(s) 262, 1667
PsuI RGATCY 4 cut(s) 427, 1195, 2109, 2827
PvuII CAGCTG 1 cut(s) 879
RsaI GTAC 7 cut(s) 33, 1361, 1453, 1765, 2013, 2282, 2820
RsaNI GTAC 7 cut(s) 32, 1360, 1452, 1764, 2012, 2281, 2819
RseI CAYNNNNRTG 1 cut(s) 1028
SatI GCNGC 3 cut(s) 877, 880, 1934
Sau96I GGNCC 3 cut(s) 1188, 1409, 2093
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 5 cut(s) 1061, 1226, 1962, 2226, 2709
ScrFI CCNGG 9 cut(s) 395, 474, 633, 790, 921, 1118, 2118, 2410, 2500
SduI GDGCHC 3 cut(s) 777, 1331, 2811
SexAI ACCWGGT 1 cut(s) 393
SfaNI GCATC 6 cut(s) 468, 648, 965, 1280, 1878, 2340
SfcI CTRYAG 3 cut(s) 258, 1663, 2046
Sfr274I CTCGAG 2 cut(s) 1248, 2379
SfuI TTCGAA 2 cut(s) 552, 1156
SinI GGWCC 2 cut(s) 1188, 1409
SlaI CTCGAG 2 cut(s) 1248, 2379
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 4 cut(s) 1018, 1248, 1767, 2379
SmoI CTYRAG 4 cut(s) 1018, 1248, 1767, 2379
SphI GCATGC 1 cut(s) 1869
SsiI CCGC 6 cut(s) 301, 425, 899, 1931, 2353, 2649
SspI AATATT 1 cut(s) 641
SspMI CTAG 6 cut(s) 72, 557, 1500, 2039, 2636, 2937
StyD4I CCNGG 9 cut(s) 393, 472, 631, 788, 919, 1116, 2116, 2408, 2498
StyI CCWWGG 1 cut(s) 1405
TaaI ACNGT 8 cut(s) 223, 370, 987, 1153, 1289, 1696, 2542, 2876
TaiI ACGT 1 cut(s) 15
TaqI TCGA 7 cut(s) 105, 552, 1058, 1156, 1249, 1938, 2380
TaqII GACCGA 1 cut(s) 543
TatI WGTACW 5 cut(s) 31, 1359, 1451, 1763, 2280
TscAI CASTG 7 cut(s) 274, 375, 815, 853, 1078, 1156, 2881
TseFI GTSAC 3 cut(s) 208, 1223, 1976
TseI GCWGC 3 cut(s) 876, 879, 1933
Tsp45I GTSAC 3 cut(s) 208, 1223, 1976
TspGWI ACGGA 2 cut(s) 1208, 2938
TspRI CASTG 7 cut(s) 274, 375, 815, 853, 1078, 1156, 2881
Vha464I CTTAAG 1 cut(s) 1018
VpaK11BI GGWCC 2 cut(s) 1188, 1409
VspI ATTAAT 3 cut(s) 855, 888, 1389
XapI RAATTY 8 cut(s) 688, 700, 760, 1394, 1571, 1732, 2590, 2783
XbaI TCTAGA 2 cut(s) 1499, 2635
XceI RCATGY 4 cut(s) 1869, 2287, 2335, 2566
XhoI CTCGAG 2 cut(s) 1248, 2379
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 6 cut(s) 72, 557, 1500, 2039, 2636, 2937
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 2 cut(s) 1871, 2581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.