RLG00000021257

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000412
Physical Location & Seq
Reverse (-)
73441124 .. 73449610
8487 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021257

Sequence Viewer

Length: 2310 bp
ATGAAGTTTATCCAAAGAACAAAGATAACAAAAGCAAATGGCATTATTATATGCTTTGCAAATGGCATGCTATCTTCCATCTTCTTATCATATGCACTCTTCTTGTGCTTTTCTCACCCCTTCAGCTCTGCTAGAGATACAATAACCAAAGATAATCCAATTAAAGATGATGGTTCAGAAGGTCTTGTTTCAGCTGGAGGAAAATTTGAATTGGGATTCTTTCCCCTACATGGAAGAGGAAGCTCAGGCAGTTACAGAAGATATGTTGGCATATGGTATCACAAACTGACTCCAAGGACAGTTATATGGGTTGCCAACAGAGACAATCCACTGCTTGCCAATCACACTGGAGTTCTTGCAATCAAACAGGGTACCCTCCAAGTGTTGGATATAACTTCTAGAAAGTCTTTATGGTCGGCAGAGATCAAAGTAATACAGTCTTTCAATCGGACAGTGAAACTCAAGGATGATGGGAACTTGGTGTTAAGTGATACTGATGCACATTTGGCAGAAGTTATGTGGCAAAGCTTTGAAATTCCTACTGATACATTCATTCCTGGGATGATAATGGATAAAAACTTAATTTTGACTTCATGGAACGACAAAGATAACCCAGGACCCGGGAACTTTACCTTTAAAGTCGATGAAGACAACCAGTATATCATCAAGAAATCAGCTACAGATTACTGGAGAAGTAGAGCACCAAATGGTAATTCTTTCGGCTCAGATGAAATGCCTGCTGCAGTTGCTTGCCAAATTTTCAATGCCTCGGGTTCACACTCTTACAATGAGACAAAAGACCTGCCACGATCAGGGTACAATTATACAAGATTGGTAATAGATTCTACTGGAAAGCTGAACTTTCTTGTGTACACACACAACTGGAGCTCGACATGGAGTGAGCCAAGTGATTATTGCAGTGTGTTTAACCGTTGTGGGGACTTCGGTATCTGCAATAGTACTAATAAGCCAATCTGTCGATGTTTGCGAGGTTTCAAGCCCCAGCTTCAACAGAACTGGAACTCTGGAGATTTTTCAGGTGGATGCTTAAGAAAGTCCCCATTATGTGCTAACAGGAGTGAGACGTTCTTCAGTTTGAAGGTCAAGAAAGCGCCAAATCCAGACCCAGAGACCTATGTTGCCAATGAAACAGAATGCAGGAACATATGCTTGAAAAAGTGCGAATGCCTGGCTTACTCATTAGCTACAGGTGAAAAGAGAAATCGGTACACTACCACGTGTTTAATATGGGCTTCGAGGGTCCTAAGTAATCTTGAAGAGTCTTCTTCTGGCCAGAACCTCTATGCTCGCATTGCCGGGTCTGATTTAGAAGGAAATCATCAAGAGCCAACTAAACCGTCATTGGAAGATCATTCAAGAAGAAAAATTCCATTGTTTCTGATAGTTGTTGCAGTGCTTGGGGAGTTTCCTGGAGGTCAAGAAATTGCAGTTAAGAGGCTATCAAGGGTTTCAGTACAAGGCTTACAGGAATTTAAGAACGAGATGGTACTTAAAGGATATTGCATGAAAGGAGAAGAAAAGATTTTACTCTATGAATATATGTCCAACAAAAGCTTAGACTTCTTCATATTTGATCACACTAAAAGTGTGTTTCTCAATTGGGATATGCGCTTTAACATCATTTTGGGAATTGCTCGAGGACTTCTTTATCTTCATCAAGATTCCAGATTGAGGATCATTCATAGAGATTTGAAGACCAGCAACATTCTCTTAGACGAGGAGATGAACCACAAAATATCAGACTTTGGCTTGGCGAGGATTGTTGGAGGCAAAGAAACTAAGGCAAACACAAACACTGTAGTTGGAACTTATGGGTACATGTCTCCAGAGTATGCATTAGATGAAACGTTCTCGGTCAAGTCAGATGTCTTTAGCTTTGGTGTTGCATGGAGACTTTGGACAGAAAACAAGGGGTTGGATTTGATAGACATGAATTTGCATGAAAGTTGCAACAAAAATCAGTTCCTTGCATGTGTGAATGTTGGGCTCTTATGCGTGCAAGAAGACCCTAATGACCGGCCTGCCATGTCAACTGTTGTTACCTTGCTTGATAGTGGAACTGCAATCCCTCCAACTCCCAAGCAACCCGCCTTTGTCATAAAGAGAGGCAACTCCAGCACAGCTTCTACTTCCAGTAAGCAAGAAGCTTACCCTGAAATTACTAATACTCTAGAAGAAGATGACGATGATGATGACAATGATTCTCATGTTGATGATAATATATGGTATGGTAAATTGTCTCCGAGCACCCTGCAGCATCAAGTCTTTTGCCGGATCAACATGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

770

Amino Acids

86.49

Weight (kDa)

6.62

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 100 - 194 2.3e-22 D-mannose binding lectin
S_locus_glycop PF00954 256 - 335 3.8e-17 S-locus glycoprotein domain
PAN_2 PF08276 356 - 422 4.2e-10 PAN-like domain
PK_Tyr_Ser-Thr PF07714 499 - 688 2.2e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 503 - 636 1.5e-27 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 810
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 385
AciI CCGC 1 cut(s) 2109
AclI AACGTT 1 cut(s) 1868
AclWI GGATC 2 cut(s) 1703, 2303
AcoI YGGCCR 1 cut(s) 1291
AcsI RAATTY 6 cut(s) 203, 534, 756, 1386, 1490, 1954
AcuI CTGAAG 2 cut(s) 106, 1075
AcvI CACGTG 1 cut(s) 1239
AfaI GTAC 8 cut(s) 373, 818, 872, 961, 1229, 1476, 1509, 1838
AfiI CCNNNNNNNGG 6 cut(s) 230, 385, 620, 812, 937, 1692
AflII CTTAAG 1 cut(s) 1048
AflIII ACRYGT 2 cut(s) 1238, 1839
AjnI CCWGG 4 cut(s) 556, 613, 1188, 1429
AjuI GAANNNNNNNTTGG 2 cut(s) 697, 729
Alw21I GWGCWC 3 cut(s) 703, 890, 2270
Alw26I GTCTC 7 cut(s) 315, 785, 1076, 1124, 1848, 1906, 2265
AlwI GGATC 2 cut(s) 1703, 2303
Ama87I CYCGRG 3 cut(s) 620, 769, 1656
AoxI GGCC 2 cut(s) 1291, 2039
ApeKI GCWGC 2 cut(s) 740, 2275
ApoI RAATTY 6 cut(s) 203, 534, 756, 1386, 1490, 1954
Asp718I GGTACC 1 cut(s) 371
AspLEI GCGC 2 cut(s) 1114, 1632
AspS9I GGNCC 2 cut(s) 617, 1261
AsuC2I CCSGG 3 cut(s) 621, 622, 1318
AsuHPI GGTGA 2 cut(s) 107, 1223
AvaI CYCGRG 3 cut(s) 620, 769, 1656
AvaII GGWCC 2 cut(s) 617, 1261
BaeI ACNNNNGTAYC 2 cut(s) 931, 964
BalI TGGCCA 1 cut(s) 1293
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 2 cut(s) 890, 2010
BbrPI CACGTG 1 cut(s) 1239
BbsI GAAGAC 4 cut(s) 654, 1275, 1721, 2031
Bbv12I GWGCWC 3 cut(s) 703, 890, 2270
BbvI GCAGC 2 cut(s) 727, 2287
BccI CCATC 4 cut(s) 86, 164, 464, 1498
BcgI CGANNNNNNTGC 2 cut(s) 2254, 2288
BciT130I CCWGG 4 cut(s) 558, 615, 1190, 1431
BclI TGATCA 1 cut(s) 1594
BcnI CCSGG 3 cut(s) 621, 622, 1318
BcoDI GTCTC 7 cut(s) 315, 785, 1076, 1124, 1848, 1906, 2265
BfaI CTAG 3 cut(s) 132, 399, 2192
BfmI CTRYAG 5 cut(s) 678, 741, 1206, 1818, 2273
BfoI RGCGCY 1 cut(s) 1115
BfrI CTTAAG 1 cut(s) 1048
BfuAI ACCTGC 1 cut(s) 810
BisI GCNGC 2 cut(s) 741, 2276
BlsI GCNGC 2 cut(s) 742, 2277
BmcAI AGTACT 1 cut(s) 961
Bme1390I CCNGG 7 cut(s) 558, 615, 621, 622, 1190, 1318, 1431
Bme18I GGWCC 2 cut(s) 617, 1261
BmeT110I CYCGRG 3 cut(s) 620, 769, 1656
BmgT120I GGNCC 2 cut(s) 617, 1261
BmiI GGNNCC 3 cut(s) 373, 619, 1262
BmrFI CCNGG 7 cut(s) 558, 615, 621, 622, 1190, 1318, 1431
BmsI GCATC 3 cut(s) 487, 1034, 2287
BpiI GAAGAC 4 cut(s) 654, 1275, 1721, 2031
BpmI CTGGAG 8 cut(s) 216, 369, 709, 904, 1047, 1452, 1830, 2119
Bpu10I CCTNAGC 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 3 cut(s) 621, 622, 1318
BsaAI YACGTR 1 cut(s) 1239
BsaI GGTCTC 1 cut(s) 1124
BsaJI CCNNGG 5 cut(s) 293, 557, 613, 620, 768
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 385, 620, 812, 937, 1692
Bse118I RCCGGY 1 cut(s) 2037
Bse1I ACTGG 7 cut(s) 352, 655, 692, 853, 887, 1022, 2154
Bse3DI GCAATG 1 cut(s) 1311
BseBI CCWGG 4 cut(s) 558, 615, 1190, 1431
BseDI CCNNGG 5 cut(s) 293, 557, 613, 620, 768
BseGI GGATG 3 cut(s) 472, 567, 1049
BseLI CCNNNNNNNGG 6 cut(s) 230, 385, 620, 812, 937, 1692
BseMI GCAATG 1 cut(s) 1311
BseMII CTCAG 2 cut(s) 258, 738
BseNI ACTGG 7 cut(s) 352, 655, 692, 853, 887, 1022, 2154
BseRI GAGGAG 1 cut(s) 1754
BseXI GCAGC 2 cut(s) 727, 2287
BseYI CCCAGC 1 cut(s) 1002
BshFI GGCC 2 cut(s) 1293, 2041
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 3 cut(s) 703, 890, 2270
BsiHKCI CYCGRG 3 cut(s) 620, 769, 1656
BsiSI CCGG 4 cut(s) 621, 1317, 2038, 2293
BslFI GGGAC 2 cut(s) 953, 1042
BslI CCNNNNNNNGG 6 cut(s) 230, 385, 620, 812, 937, 1692
BsmAI GTCTC 7 cut(s) 315, 785, 1076, 1124, 1848, 1906, 2265
BsmBI CGTCTC 1 cut(s) 1076
BsmFI GGGAC 2 cut(s) 953, 1042
BsmI GAATGC 2 cut(s) 1160, 1190
BsnI GGCC 2 cut(s) 1293, 2041
Bso31I GGTCTC 1 cut(s) 1124
BsoBI CYCGRG 3 cut(s) 620, 769, 1656
Bsp1286I GDGCHC 4 cut(s) 703, 890, 2010, 2270
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
BspACI CCGC 1 cut(s) 2109
BspANI GGCC 2 cut(s) 1293, 2041
BspCNI CTCAG 2 cut(s) 257, 737
BspLI GGNNCC 3 cut(s) 373, 619, 1262
BspMAI CTGCAG 2 cut(s) 745, 2277
BspMI ACCTGC 1 cut(s) 810
BspPI GGATC 2 cut(s) 1703, 2303
BspT107I GGYRCC 1 cut(s) 371
BspTI CTTAAG 1 cut(s) 1048
BspTNI GGTCTC 1 cut(s) 1124
BsrDI GCAATG 1 cut(s) 1311
BsrFI RCCGGY 1 cut(s) 2037
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 7 cut(s) 352, 655, 692, 853, 887, 1022, 2154
BssAI RCCGGY 1 cut(s) 2037
BssECI CCNNGG 5 cut(s) 293, 557, 613, 620, 768
BssMI GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 4 cut(s) 558, 615, 1190, 1431
Bst4CI ACNGT 7 cut(s) 301, 438, 454, 932, 1359, 1819, 2056
Bst6I CTCTTC 3 cut(s) 104, 229, 1272
BstAFI CTTAAG 1 cut(s) 1048
BstAUI TGTACA 1 cut(s) 870
BstBAI YACGTR 1 cut(s) 1239
BstC8I GCNNGC 7 cut(s) 68, 336, 738, 751, 1309, 2018, 2043
BstDEI CTNAG 6 cut(s) 244, 724, 1265, 1576, 1732, 1800
BstF5I GGATG 3 cut(s) 472, 567, 1049
BstH2I RGCGCY 1 cut(s) 1115
BstHHI GCGC 2 cut(s) 1114, 1632
BstKTI GATC 7 cut(s) 426, 812, 1372, 1597, 1698, 2298, 2307
BstMAI GTCTC 7 cut(s) 315, 785, 1076, 1124, 1848, 1906, 2265
BstMBI GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
BstMWI GCNNNNNNNGC 4 cut(s) 506, 746, 1313, 2136
BstNI CCWGG 4 cut(s) 558, 615, 1190, 1431
BstNSI RCATGY 3 cut(s) 70, 1843, 1995
BstSCI CCNGG 7 cut(s) 556, 613, 619, 620, 1188, 1316, 1429
BstSFI CTRYAG 5 cut(s) 678, 741, 1206, 1818, 2273
BstV1I GCAGC 2 cut(s) 727, 2287
BstV2I GAAGAC 4 cut(s) 654, 1275, 1721, 2031
BsuRI GGCC 2 cut(s) 1293, 2041
BtsCI GGATG 3 cut(s) 472, 567, 1049
BtsI GCAGTG 3 cut(s) 329, 925, 1419
BtsIMutI CAGTG 6 cut(s) 329, 345, 459, 925, 1419, 1815
BveI ACCTGC 1 cut(s) 810
Cac8I GCNNGC 7 cut(s) 68, 336, 738, 751, 1309, 2018, 2043
CfoI GCGC 2 cut(s) 1114, 1632
Cfr10I RCCGGY 1 cut(s) 2037
Cfr13I GGNCC 2 cut(s) 617, 1261
Cfr9I CCCGGG 1 cut(s) 620
Csp6I GTAC 8 cut(s) 372, 817, 871, 960, 1228, 1475, 1508, 1837
CviQI GTAC 8 cut(s) 372, 817, 871, 960, 1228, 1475, 1508, 1837
DdeI CTNAG 6 cut(s) 244, 724, 1265, 1576, 1732, 1800
DpnI GATC 7 cut(s) 425, 811, 1371, 1596, 1697, 2297, 2306
DpnII GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
DraI TTTAAA 1 cut(s) 637
EaeI YGGCCR 1 cut(s) 1291
Eam1104I CTCTTC 3 cut(s) 104, 229, 1272
EarI CTCTTC 3 cut(s) 104, 229, 1272
Ecl136II GAGCTC 1 cut(s) 888
Eco130I CCWWGG 1 cut(s) 293
Eco24I GRGCYC 2 cut(s) 890, 2010
Eco31I GGTCTC 1 cut(s) 1124
Eco47I GGWCC 2 cut(s) 617, 1261
Eco53kI GAGCTC 1 cut(s) 888
Eco57I CTGAAG 2 cut(s) 106, 1075
Eco72I CACGTG 1 cut(s) 1239
Eco88I CYCGRG 3 cut(s) 620, 769, 1656
EcoICRI GAGCTC 1 cut(s) 888
EcoO109I RGGNCCY 2 cut(s) 617, 1261
EcoRII CCWGG 4 cut(s) 556, 613, 1188, 1429
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 1858
EcoT38I GRGCYC 2 cut(s) 890, 2010
ErhI CCWWGG 1 cut(s) 293
Esp3I CGTCTC 1 cut(s) 1076
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FaqI GGGAC 2 cut(s) 953, 1042
FauI CCCGC 1 cut(s) 2116
FauNDI CATATG 3 cut(s) 91, 272, 1166
FbaI TGATCA 1 cut(s) 1594
Fnu4HI GCNGC 2 cut(s) 741, 2276
FokI GGATG 3 cut(s) 479, 574, 1056
FriOI GRGCYC 2 cut(s) 890, 2010
Fsp4HI GCNGC 2 cut(s) 741, 2276
FspBI CTAG 3 cut(s) 132, 399, 2192
GlaI GCGC 2 cut(s) 1113, 1631
GluI GCNGC 2 cut(s) 741, 2276
GsaI CCCAGC 1 cut(s) 1006
GsuI CTGGAG 8 cut(s) 216, 369, 709, 904, 1047, 1452, 1830, 2119
HaeII RGCGCY 1 cut(s) 1115
HaeIII GGCC 2 cut(s) 1293, 2041
HapII CCGG 4 cut(s) 621, 1317, 2038, 2293
HhaI GCGC 2 cut(s) 1114, 1632
Hin6I GCGC 2 cut(s) 1112, 1630
HinP1I GCGC 2 cut(s) 1112, 1630
HincII GTYRAC 1 cut(s) 2052
HindII GTYRAC 1 cut(s) 2052
HindIII AAGCTT 3 cut(s) 526, 1573, 2166
HinfI GANTC 6 cut(s) 216, 289, 842, 1280, 1682, 2222
HpaII CCGG 4 cut(s) 621, 1317, 2038, 2293
HphI GGTGA 2 cut(s) 107, 1223
Hpy166II GTNNAC 5 cut(s) 776, 871, 873, 1230, 2052
Hpy188I TCNGA 9 cut(s) 178, 450, 727, 1324, 1401, 1762, 1885, 2265, 2309
Hpy8I GTNNAC 5 cut(s) 776, 871, 873, 1230, 2052
HpyAV CCTTC 4 cut(s) 130, 173, 1093, 1325
HpyCH4III ACNGT 7 cut(s) 301, 438, 454, 932, 1359, 1819, 2056
HpyCH4IV ACGT 3 cut(s) 1085, 1238, 1868
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 746, 1313, 2136
HpyF3I CTNAG 6 cut(s) 244, 724, 1265, 1576, 1732, 1800
HpySE526I ACGT 3 cut(s) 1085, 1238, 1868
HspAI GCGC 2 cut(s) 1112, 1630
KpnI GGTACC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 1594
Kzo9I GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
LmnI GCTCC 1 cut(s) 885
Lsp1109I GCAGC 2 cut(s) 727, 2287
LweI GCATC 3 cut(s) 487, 1034, 2287
MaeI CTAG 3 cut(s) 132, 399, 2192
MaeII ACGT 3 cut(s) 1085, 1238, 1868
MaeIII GTNAC 2 cut(s) 251, 2059
MalI GATC 7 cut(s) 425, 811, 1371, 1596, 1697, 2297, 2306
MboI GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
MfeI CAATTG 1 cut(s) 1618
MhlI GDGCHC 4 cut(s) 703, 890, 2010, 2270
MlsI TGGCCA 1 cut(s) 1293
MluNI TGGCCA 1 cut(s) 1293
MlyI GAGTC 2 cut(s) 283, 1289
MmeI TCCRAC 6 cut(s) 366, 1590, 1765, 1804, 1917, 2117
Mox20I TGGCCA 1 cut(s) 1293
Mph1103I ATGCAT 1 cut(s) 1858
MscI TGGCCA 1 cut(s) 1293
MslI CAYNNNNRTG 1 cut(s) 2232
Msp20I TGGCCA 1 cut(s) 1293
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 1048
MspI CCGG 4 cut(s) 621, 1317, 2038, 2293
MspR9I CCNGG 7 cut(s) 558, 615, 621, 622, 1190, 1318, 1431
MunI CAATTG 1 cut(s) 1618
Mva1269I GAATGC 2 cut(s) 1160, 1190
MvaI CCWGG 4 cut(s) 558, 615, 1190, 1431
MwoI GCNNNNNNNGC 4 cut(s) 506, 746, 1313, 2136
NciI CCSGG 3 cut(s) 621, 622, 1318
NdeI CATATG 3 cut(s) 91, 272, 1166
NdeII GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
NlaIV GGNNCC 3 cut(s) 373, 619, 1262
NsiI ATGCAT 1 cut(s) 1858
NspI RCATGY 3 cut(s) 70, 1843, 1995
PaeI GCATGC 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 1656
PciI ACATGT 1 cut(s) 1839
PcsI WCGNNNNNNNCGW 1 cut(s) 985
PctI GAATGC 2 cut(s) 1160, 1190
PfeI GAWTC 4 cut(s) 216, 842, 1682, 2222
PflMI CCANNNNNTGG 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 1429
PkrI GCNGC 2 cut(s) 742, 2277
PleI GAGTC 2 cut(s) 283, 1288
PmaCI CACGTG 1 cut(s) 1239
PmlI CACGTG 1 cut(s) 1239
PpsI GAGTC 2 cut(s) 283, 1288
Ppu21I YACGTR 1 cut(s) 1239
PpuMI RGGWCCY 2 cut(s) 617, 1261
PscI ACATGT 1 cut(s) 1839
Psp124BI GAGCTC 1 cut(s) 890
Psp1406I AACGTT 1 cut(s) 1868
Psp5II RGGWCCY 2 cut(s) 617, 1261
Psp6I CCWGG 4 cut(s) 556, 613, 1188, 1429
PspCI CACGTG 1 cut(s) 1239
PspFI CCCAGC 1 cut(s) 1002
PspGI CCWGG 4 cut(s) 556, 613, 1188, 1429
PspN4I GGNNCC 3 cut(s) 373, 619, 1262
PspPI GGNCC 2 cut(s) 617, 1261
PspPPI RGGWCCY 2 cut(s) 617, 1261
PspXI VCTCGAGB 1 cut(s) 1656
PstI CTGCAG 2 cut(s) 745, 2277
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 8 cut(s) 373, 818, 872, 961, 1229, 1476, 1509, 1838
RsaNI GTAC 8 cut(s) 372, 817, 871, 960, 1228, 1475, 1508, 1837
RseI CAYNNNNRTG 1 cut(s) 2232
SacI GAGCTC 1 cut(s) 890
SatI GCNGC 2 cut(s) 741, 2276
Sau3AI GATC 7 cut(s) 423, 809, 1369, 1594, 1695, 2295, 2304
Sau96I GGNCC 2 cut(s) 617, 1261
ScaI AGTACT 1 cut(s) 961
SchI GAGTC 2 cut(s) 283, 1289
ScrFI CCNGG 7 cut(s) 558, 615, 621, 622, 1190, 1318, 1431
SduI GDGCHC 4 cut(s) 703, 890, 2010, 2270
SfaNI GCATC 3 cut(s) 487, 1034, 2287
SfcI CTRYAG 5 cut(s) 678, 741, 1206, 1818, 2273
Sfr274I CTCGAG 1 cut(s) 1656
SinI GGWCC 2 cut(s) 617, 1261
SlaI CTCGAG 1 cut(s) 1656
SmaI CCCGGG 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 2232
SmlI CTYRAG 3 cut(s) 461, 1048, 1656
SmoI CTYRAG 3 cut(s) 461, 1048, 1656
SphI GCATGC 1 cut(s) 70
SsiI CCGC 1 cut(s) 2109
SspMI CTAG 3 cut(s) 132, 399, 2192
SstI GAGCTC 1 cut(s) 890
StyD4I CCNGG 7 cut(s) 556, 613, 619, 620, 1188, 1316, 1429
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 7 cut(s) 301, 438, 454, 932, 1359, 1819, 2056
TaiI ACGT 3 cut(s) 1088, 1241, 1871
TaqI TCGA 5 cut(s) 642, 890, 979, 1256, 1657
TaqII GACCGA 1 cut(s) 1864
TatI WGTACW 3 cut(s) 870, 959, 1474
TfiI GAWTC 4 cut(s) 216, 842, 1682, 2222
TscAI CASTG 6 cut(s) 336, 352, 459, 925, 1419, 1822
TseI GCWGC 2 cut(s) 740, 2275
TspMI CCCGGG 1 cut(s) 620
TspRI CASTG 6 cut(s) 336, 352, 459, 925, 1419, 1822
Van91I CCANNNNNTGG 1 cut(s) 385
Vha464I CTTAAG 1 cut(s) 1048
VpaK11BI GGWCC 2 cut(s) 617, 1261
XapI RAATTY 6 cut(s) 203, 534, 756, 1386, 1490, 1954
XbaI TCTAGA 2 cut(s) 398, 2191
XceI RCATGY 3 cut(s) 70, 1843, 1995
XhoI CTCGAG 1 cut(s) 1656
XmaI CCCGGG 1 cut(s) 620
XspI CTAG 3 cut(s) 132, 399, 2192
ZrmI AGTACT 1 cut(s) 961
Zsp2I ATGCAT 1 cut(s) 1858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.