Rroxscaffold_4G00321430

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000412
Physical Location & Seq
Reverse (-)
50913208 .. 50932081
18874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321430.1

Sequence Viewer

Length: 1287 bp
ATGAAGAGGTGGAGAACAAAAAAACGAGTAGTAATTCAACATGTTGGATTTGGTCACAAGATGTCACCAATCTTCAGAGGACTATGCCGGTGGCCGAATCTACGTGTTCGTGTAGCAGCTTTCGTATATAGGCCACTCAAGGAAAGGCTAAAAAGGCCGGAGATTGAGGAGAAGAAGGTCGAAGAATCAACAGCGAGGAGCTGTGGAAGTTGTGGCACAAACCTGATCCCTTATCCCTTAAGCACTGGACCAAAGTGTGGTGATGTCACCTACTATAGTTTCCACTGCAATATTTCAACTGGCCAGCTGAGCTTTGAGGCACCAAGCGGCACCTACCATGTCACAAGCATCAACGCGGACACACAAACATTTGTCATCCAAGCTAATGATGCAGATGAATGTAGAGATAAAAAATTTCTGAAGCTCATCCAGTCTTCTCCATATAATGTGACAAACATGTGCAATGCTGATCCAACCCGTTTTAGTCCTGACTTGTCATTTAAAGGAGGATATGAAATTGAAGTTGCTTGGGAGTCACCCTTGGAACCACCTTGTTCCTCATCTACAGACTGGCCTCGTTCGATATGTGATGCTGCTCTAGATGGGAAGAATAGGTGTCTTTGCCCTGCAAACTCAAAATGGGATAGCGGGAGTTTAAATTGTACTCAAGAAGACAGGGCAAATCTTCAAAAGTGTTCAACACTTAATCACTTTTATGACAGTGAGAGAAAAGTCAAGAACTTGATTGAATCAGGCCGATTTAAGGATGACGATACGGAGGGCATTGATGTACCCTCTTTTGATTTGGAAAGCATACTGGTAGCTACAACATACTTCTCCAATGCAAATAAACTTGGACAAGGAGGATTTGGTCCTGTTTACAAGGGTAAACTTCCGGGAGGAGAAGAAATCGCTGTAAAGAGGCTCTCAAGTTGTTCAGGCCAAGGCTTAGAGGAATTCAAAAATGAAGTTTTGTTAATTGCCAAACTTCAACATCAGAATCTGGTTCGACTTTTTTGGGCTATTGTGCTGAGGGAGATGAAAAGATATCAAAAAGTATGTGTATCATTGGACTGGAATAGACGCTTTAACATCATCTCAGGAATTGCTCGGGGGCTTCTTTATCTTCACCAAGATTCTAGATTAAGGGTTATTCATAGAGATCTGAAAACCAGCAACATTCTACAGAGTGAAGAGATGAACCCCAAAATATCAGACTTCGGTTTGGCAAGGATCTTTGGAGGCAATGAAACTTCAGCAAACACCAATAGAGTAGTGGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

428

Amino Acids

48.26

Weight (kDa)

8.92

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 69 - 126 9.3e-07 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 280 - 428 1.7e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 282 - 416 4.2e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 319, 329
AccB7I CCANNNNNTGG 1 cut(s) 257
AccII CGCG 1 cut(s) 356
AciI CCGC 3 cut(s) 327, 356, 648
AclWI GGATC 3 cut(s) 220, 464, 1241
AcoI YGGCCR 2 cut(s) 92, 301
AcsI RAATTY 2 cut(s) 413, 956
AcuI CTGAAG 3 cut(s) 58, 440, 1239
AfaI GTAC 2 cut(s) 664, 792
AfiI CCNNNNNNNGG 2 cut(s) 257, 763
AflII CTTAAG 1 cut(s) 238
AflIII ACRYGT 3 cut(s) 40, 103, 456
AgsI TTSAA 8 cut(s) 38, 297, 521, 689, 699, 749, 961, 992
AluBI AGCT 7 cut(s) 119, 201, 307, 312, 383, 424, 824
AluI AGCT 7 cut(s) 119, 201, 307, 312, 383, 424, 824
AlwI GGATC 3 cut(s) 220, 464, 1241
AlwNI CAGNNNCTG 1 cut(s) 1003
Ama87I CYCGRG 1 cut(s) 1110
AoxI GGCC 7 cut(s) 92, 131, 155, 301, 572, 754, 940
ApeKI GCWGC 2 cut(s) 116, 593
ApoI RAATTY 2 cut(s) 413, 956
AspS9I GGNCC 2 cut(s) 248, 872
AsuC2I CCSGG 1 cut(s) 897
AsuHPI GGTGA 5 cut(s) 57, 259, 272, 528, 1121
AvaI CYCGRG 1 cut(s) 1110
AvaII GGWCC 2 cut(s) 248, 872
BalI TGGCCA 1 cut(s) 303
BanI GGYRCC 2 cut(s) 319, 329
BbsI GAAGAC 2 cut(s) 426, 678
BbvCI CCTCAGC 1 cut(s) 1031
BbvI GCAGC 2 cut(s) 128, 580
BccI CCATC 1 cut(s) 596
BcnI CCSGG 1 cut(s) 897
BfaI CTAG 2 cut(s) 599, 1140
BfmI CTRYAG 3 cut(s) 274, 564, 1184
BfrI CTTAAG 1 cut(s) 238
BglII AGATCT 1 cut(s) 1162
BisI GCNGC 3 cut(s) 117, 328, 594
BlpI GCTNAGC 1 cut(s) 308
BlsI GCNGC 3 cut(s) 118, 329, 595
Bme1390I CCNGG 1 cut(s) 897
Bme18I GGWCC 2 cut(s) 248, 872
BmeT110I CYCGRG 1 cut(s) 1110
BmgT120I GGNCC 2 cut(s) 248, 872
BmiI GGNNCC 3 cut(s) 321, 331, 546
BmrFI CCNGG 1 cut(s) 897
BmsI GCATC 3 cut(s) 357, 379, 580
BpiI GAAGAC 2 cut(s) 426, 678
Bpu10I CCTNAGC 1 cut(s) 1031
Bpu1102I GCTNAGC 1 cut(s) 308
BpuEI CTTGAG 3 cut(s) 122, 651, 913
BpuMI CCSGG 1 cut(s) 897
BsaAI YACGTR 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 540, 943
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 763
Bse118I RCCGGY 1 cut(s) 87
Bse1I ACTGG 6 cut(s) 250, 304, 430, 575, 822, 1079
Bse3DI GCAATG 2 cut(s) 469, 1252
BseDI CCNNGG 2 cut(s) 540, 943
BseGI GGATG 3 cut(s) 375, 426, 772
BseLI CCNNNNNNNGG 2 cut(s) 257, 763
BseMI GCAATG 2 cut(s) 469, 1252
BseMII CTCAG 3 cut(s) 299, 1022, 1113
BseNI ACTGG 6 cut(s) 250, 304, 430, 575, 822, 1079
BseRI GAGGAG 3 cut(s) 182, 211, 915
BseXI GCAGC 2 cut(s) 128, 580
Bsh1236I CGCG 1 cut(s) 356
BshFI GGCC 7 cut(s) 94, 133, 157, 303, 574, 756, 942
BshNI GGYRCC 2 cut(s) 319, 329
BsiHKCI CYCGRG 1 cut(s) 1110
BsiSI CCGG 3 cut(s) 88, 158, 896
BslI CCNNNNNNNGG 2 cut(s) 257, 763
BsnI GGCC 7 cut(s) 94, 133, 157, 303, 574, 756, 942
BsoBI CYCGRG 1 cut(s) 1110
Bsp143I GATC 4 cut(s) 225, 469, 1162, 1233
Bsp1720I GCTNAGC 1 cut(s) 308
BspACI CCGC 3 cut(s) 327, 356, 648
BspANI GGCC 7 cut(s) 94, 133, 157, 303, 574, 756, 942
BspCNI CTCAG 3 cut(s) 300, 1023, 1112
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 3 cut(s) 321, 331, 546
BspPI GGATC 3 cut(s) 220, 464, 1241
BspT107I GGYRCC 2 cut(s) 319, 329
BspTI CTTAAG 1 cut(s) 238
BsrDI GCAATG 2 cut(s) 469, 1252
BsrFI RCCGGY 1 cut(s) 87
BsrI ACTGG 6 cut(s) 250, 304, 430, 575, 822, 1079
BssAI RCCGGY 1 cut(s) 87
BssECI CCNNGG 2 cut(s) 540, 943
BssMI GATC 4 cut(s) 225, 469, 1162, 1233
BssT1I CCWWGG 2 cut(s) 540, 943
Bst4CI ACNGT 1 cut(s) 722
Bst6I CTCTTC 1 cut(s) 1188
BstAFI CTTAAG 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 4 cut(s) 308, 949, 1031, 1099
BstF5I GGATG 3 cut(s) 375, 426, 772
BstFNI CGCG 1 cut(s) 356
BstKTI GATC 4 cut(s) 228, 472, 1165, 1236
BstMBI GATC 4 cut(s) 225, 469, 1162, 1233
BstMWI GCNNNNNNNGC 3 cut(s) 154, 309, 389
BstNSI RCATGY 2 cut(s) 44, 460
BstSCI CCNGG 1 cut(s) 895
BstSFI CTRYAG 3 cut(s) 274, 564, 1184
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 2 cut(s) 128, 580
BstV2I GAAGAC 2 cut(s) 426, 678
BstX2I RGATCY 2 cut(s) 1162, 1233
BstYI RGATCY 2 cut(s) 1162, 1233
BsuRI GGCC 7 cut(s) 94, 133, 157, 303, 574, 756, 942
BtsCI GGATG 3 cut(s) 375, 426, 772
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 3 cut(s) 243, 283, 727
Cac8I GCNNGC 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 1003
Cfr10I RCCGGY 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 248, 872
CseI GACGC 1 cut(s) 1092
Csp6I GTAC 2 cut(s) 663, 791
CviAII CATG 3 cut(s) 41, 338, 457
CviQI GTAC 2 cut(s) 663, 791
DdeI CTNAG 4 cut(s) 308, 949, 1031, 1099
DpnI GATC 4 cut(s) 227, 471, 1164, 1235
DpnII GATC 4 cut(s) 225, 469, 1162, 1233
DraI TTTAAA 2 cut(s) 502, 657
EaeI YGGCCR 2 cut(s) 92, 301
Eam1104I CTCTTC 1 cut(s) 1188
EarI CTCTTC 1 cut(s) 1188
Eco130I CCWWGG 2 cut(s) 540, 943
Eco32I GATATC 1 cut(s) 1049
Eco47I GGWCC 2 cut(s) 248, 872
Eco57I CTGAAG 3 cut(s) 58, 440, 1239
Eco88I CYCGRG 1 cut(s) 1110
EcoRI GAATTC 1 cut(s) 956
EcoRV GATATC 1 cut(s) 1049
EcoT14I CCWWGG 2 cut(s) 540, 943
ErhI CCWWGG 2 cut(s) 540, 943
FaeI CATG 3 cut(s) 44, 341, 460
FatI CATG 3 cut(s) 40, 337, 456
FauI CCCGC 1 cut(s) 641
Fnu4HI GCNGC 3 cut(s) 117, 328, 594
FokI GGATG 3 cut(s) 362, 413, 779
Fsp4HI GCNGC 3 cut(s) 117, 328, 594
FspBI CTAG 2 cut(s) 599, 1140
GluI GCNGC 3 cut(s) 117, 328, 594
HaeIII GGCC 7 cut(s) 94, 133, 157, 303, 574, 756, 942
HapII CCGG 3 cut(s) 88, 158, 896
HgaI GACGC 1 cut(s) 1092
Hin1II CATG 3 cut(s) 44, 341, 460
HinfI GANTC 6 cut(s) 97, 185, 533, 749, 1000, 1136
HpaII CCGG 3 cut(s) 88, 158, 896
HphI GGTGA 5 cut(s) 57, 259, 272, 528, 1121
Hpy166II GTNNAC 2 cut(s) 880, 890
Hpy188I TCNGA 5 cut(s) 77, 420, 999, 1167, 1216
Hpy188III TCNNGA 6 cut(s) 488, 599, 668, 736, 1101, 1140
Hpy8I GTNNAC 2 cut(s) 880, 890
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 1 cut(s) 722
HpyCH4IV ACGT 1 cut(s) 103
HpyCH4V TGCA 5 cut(s) 288, 392, 462, 629, 845
HpyF10VI GCNNNNNNNGC 3 cut(s) 154, 309, 389
HpyF3I CTNAG 4 cut(s) 308, 949, 1031, 1099
HpySE526I ACGT 1 cut(s) 103
Hsp92II CATG 3 cut(s) 44, 341, 460
Kzo9I GATC 4 cut(s) 225, 469, 1162, 1233
LmnI GCTCC 1 cut(s) 198
Lsp1109I GCAGC 2 cut(s) 128, 580
LweI GCATC 3 cut(s) 357, 379, 580
MaeI CTAG 2 cut(s) 599, 1140
MaeII ACGT 1 cut(s) 103
MaeIII GTNAC 6 cut(s) 53, 63, 265, 340, 448, 534
MalI GATC 4 cut(s) 227, 471, 1164, 1235
MboI GATC 4 cut(s) 225, 469, 1162, 1233
MflI RGATCY 2 cut(s) 1162, 1233
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 7 cut(s) 33, 413, 516, 658, 956, 978, 1104
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 1 cut(s) 542
MmeI TCCRAC 2 cut(s) 25, 497
Mox20I TGGCCA 1 cut(s) 303
MscI TGGCCA 1 cut(s) 303
MseI TTAA 8 cut(s) 239, 501, 656, 705, 762, 977, 1089, 1145
MslI CAYNNNNRTG 1 cut(s) 714
Msp20I TGGCCA 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 307
MspCI CTTAAG 1 cut(s) 238
MspI CCGG 3 cut(s) 88, 158, 896
MspR9I CCNGG 1 cut(s) 897
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 3 cut(s) 154, 309, 389
NciI CCSGG 1 cut(s) 897
NdeII GATC 4 cut(s) 225, 469, 1162, 1233
NlaIII CATG 3 cut(s) 44, 341, 460
NlaIV GGNNCC 3 cut(s) 321, 331, 546
NmuCI GTSAC 6 cut(s) 53, 63, 265, 340, 448, 534
NspI RCATGY 2 cut(s) 44, 460
PciI ACATGT 2 cut(s) 40, 456
PfeI GAWTC 5 cut(s) 97, 185, 749, 1000, 1136
PflMI CCANNNNNTGG 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 3 cut(s) 118, 329, 595
PleI GAGTC 1 cut(s) 541
PpsI GAGTC 1 cut(s) 541
Ppu21I YACGTR 1 cut(s) 104
PscI ACATGT 2 cut(s) 40, 456
PspN4I GGNNCC 3 cut(s) 321, 331, 546
PspPI GGNCC 2 cut(s) 248, 872
PstNI CAGNNNCTG 1 cut(s) 1003
PsuI RGATCY 2 cut(s) 1162, 1233
PvuII CAGCTG 1 cut(s) 307
RsaI GTAC 2 cut(s) 664, 792
RsaNI GTAC 2 cut(s) 663, 791
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 8 cut(s) 239, 501, 656, 705, 762, 977, 1089, 1145
SatI GCNGC 3 cut(s) 117, 328, 594
Sau3AI GATC 4 cut(s) 225, 469, 1162, 1233
Sau96I GGNCC 2 cut(s) 248, 872
SchI GAGTC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 897
SfaNI GCATC 3 cut(s) 357, 379, 580
SfcI CTRYAG 3 cut(s) 274, 564, 1184
SinI GGWCC 2 cut(s) 248, 872
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 4 cut(s) 137, 238, 666, 928
SmoI CTYRAG 4 cut(s) 137, 238, 666, 928
Sse9I AATT 7 cut(s) 33, 413, 516, 658, 956, 978, 1104
SsiI CCGC 3 cut(s) 327, 356, 648
SspI AATATT 1 cut(s) 292
SspMI CTAG 2 cut(s) 599, 1140
StyD4I CCNGG 1 cut(s) 895
StyI CCWWGG 2 cut(s) 540, 943
TaaI ACNGT 1 cut(s) 722
TaiI ACGT 1 cut(s) 106
TaqI TCGA 3 cut(s) 180, 581, 1009
TasI AATT 7 cut(s) 33, 413, 516, 658, 956, 978, 1104
TatI WGTACW 1 cut(s) 662
TauI GCSGC 1 cut(s) 330
TfiI GAWTC 5 cut(s) 97, 185, 749, 1000, 1136
Tru1I TTAA 8 cut(s) 239, 501, 656, 705, 762, 977, 1089, 1145
Tru9I TTAA 8 cut(s) 239, 501, 656, 705, 762, 977, 1089, 1145
TscAI CASTG 3 cut(s) 250, 290, 727
TseFI GTSAC 6 cut(s) 53, 63, 265, 340, 448, 534
TseI GCWGC 2 cut(s) 116, 593
Tsp45I GTSAC 6 cut(s) 53, 63, 265, 340, 448, 534
TspDTI ATGAA 8 cut(s) 17, 411, 528, 981, 1055, 1145, 1214, 1263
TspGWI ACGGA 1 cut(s) 791
TspRI CASTG 3 cut(s) 250, 290, 727
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 248, 872
XapI RAATTY 2 cut(s) 413, 956
XbaI TCTAGA 2 cut(s) 598, 1139
XceI RCATGY 2 cut(s) 44, 460
XcmI CCANNNNNNNNNTGG 1 cut(s) 1273
XspI CTAG 2 cut(s) 599, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.