Rmu_sc0002312.1_g000012

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002312.1
OG0000412
Physical Location & Seq
Reverse (-)
67555 .. 78631
11077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002312.1_g000012.1.cds

Sequence Viewer

Length: 3966 bp
atggataatgcattgggtgcaacagatgttaatgtagagaattttgattttgatttggaattggattggaaaccaagaaaaggaatggagtttgattatgaacaagcagcttattacttctacaatagatatggaggaaaagaggggtttagtattagaaggaaaagtcatgctaagaataagaaaactagtgaaaccacttcaagagtatttgtctgctgtaaggaaggtattcgatccaaatataagagggattacatgattagaaaatctagagcagagacaagaccaggttgccatgctgaactttatattaaattgaatagggagaacaataagttctttgttacccattttgttgaagagcacaatcatcctcttgtggtgaaagaatgttcccacatgcttccttcgcaacggaaagtacaagactgtcaaggcattgatatagacttagcacacgattttggaattggtgtcacgtctctatatgagttgatgggtaagcaagcaggtggaagagatgttgttgggtatacaaaacaagatgtgaaaaattaccttagatcaaagagacaaagaagcttggagtatggagaagctggatatattatgaaatatttttcagaccaaacattggaaaacccttcattctaccatgctatgcaattagacagtgaggagcaaataacaaacttattttgggcagatgctaaaatgatcattgattatggccaatttggggatgttgttagttttgatacgacatacaagattaacaaatctaatcgacctcttgttgtgttcgttggattcaatcaccaccgtgagactattgtatttggggtggctttaatgtatgatgaaacagcagattcatttatatggttgtttgagactttttttagggcaatgtctggaaaggctccaaagacaatatttacagatcaagatgctgcaatggcaaaggctcttcgacatgttatgccagatgcatatcataggctttgcaaatggcatatgatgcaaaatgccataaagcatgggagtagttttttgatagaggatggtggaatcacaagtattttgtcaagagatacactaacatctgacgatccagttatcgatgatggatcagaagctcttgtttcagcaggaagtgagtttgaactgggtttcttctctactactacggggaaaagatatgtgggcatatggtatcataaggtcactccaagaacagttgtatgggttgccaacagagctgaacctctaccagcagattccactggagttcttacaattgaagaagggaatctccgcgtgtttgattatgttactcggaagcgttattggtcaacagagattggaaaatcttcaccttttaacaagacagtgaagataatggattcagggaacctggtgttaagggatactggtgagttgacagcggatcctttgtggcagagctttcaaaatccaactgatacattcattcctgggatgcgaatggatagtaacttgaagttgacttcatggagcgacgaagatgaccgaagaactgggaactacaccttcgaactagatcaagaagggaagtcagagaaccagtttaccatcttaaataattcgtttcgttactggaaaagtggagagccaggtaaatatttaagcgcagatgagatgcgtcctgaggtagcttacttgttatcaaatttcagcaagaattcttctctttatacaactcagccgcaatttgattacaattatacaagattggtgatgaattctactggagtgctcaatggtttgacctggaatgacaccattaagcagtgggttgtgtttttctctgcgccaaaagatcaatgcagtgtgattaatgcttgtggaaactttggcagctgcaatgttaataataccccgctggtgtgcaaatgtttgccaggtttcaacccccagtttttagagaaatggaaatatggagatttttcagatggatgcaaaagacagtcgtccatatgtggcatgaaagataccttcttaagcataaaggtgaacaaagtgggagtctgggattcatggaccaatgttaccagtgatacagaatgcgagaatgcgtgccttggtgattgcaagtgccaggcttactcatctgattcatctgtaaatgccactgctcgaagggatgatctgaatatcactgcttgttatcaatggtccaaggatctaaatgatcttgtagaggagtctggtgacaatggaaagaatctcagtgctcgagttcccgtgcctgatttagaatcaactacaagagactgtagcccttgtggcacaactacaattccttatcctctgagcactagaaaagattgtggcgaccccatgtacttaagtttcgcgtgcaatgcctccattaatgaatttagcttccttggaccagatggcaaatcctttagggttataaatatcagccgaagtacacaacgattcattatcaaatccaaaaatatgaattattgtgattctagaaacagagtgctgaacatttccttcccgtttaaggtaatcagttggtgtagttctgaccagatgaggaaatttatttccgccatagattcatccagcagtccttcagtaatagagatgggttgggagccaccatcggaagcaaactgcactatcttagactgcagggattggccatattcaacttgcaaaacagtaagagagggagagaaaaaatgcctttgcaatacaaattttcaatggaacggcattgacttaaattgtactcaagaaggaaatctacaagagctatcaaataagagttcaacgaaaaaattgttattgtctctggttgtcgtggcagtcataagtgggatttttctggcatgcatcattggtttttggatatggagaagaaagattacaaacagacgagatcaagtaagtcgtccgcagctcgatagtgaaagacgagtcaaagaactcatagacacgagagactttaatgaagaagatgacaaaggcatagatgtacctttttttgatttgcaaagcatactagacgctacagataatttctcaattggaaacaagctcggacaaggggggtatgggcctgtttacaaggggatctttcctggaggtcaagaaattgctgtaaagaggctatcaagagtttcgggacaaggcttacaggaatttaagaatgaggtggtgctaatcgccaaacttcaacatcgtaatcttgtaagactcagaggatattgcatgaaaggagaagaaaagattttactctatgagtacatgcctaacaaaagcttagattccttcatatttggacttctttatcttcatcaagattccaggttgaggatcattcatagagatttgaaaaccagcaatgttcttttggatgaggagataaaccccaaaatatctgacttcggcttggccaggattgttggaggcaaagaaaccgaggcaaacacaaatacagtagtaggaacctatggttacatgtcgccggaatatgcattggatggaaatttctctgtcaaatcagatgtctttagtttcggtgtggttcttctagagataatcagcggaaaaaagaatgcaggattttatcagtccaaacaaactttcagtctccttagttatacatggggactctggacagaaaacaaggtgttggatttaatggacaacaatttggaagaaagttgcaacagaagtgaatttataaagtgtgtaaatgttgggctcttatgcgtacaagaagatccagttgatcgccctacaatgtcaaatgttgttaccttgcttgacagtgaaactgcaatcccaccgactcctaaacaaccagcgttttttatacggagaggcaactctagcacagcttcctcttctactaaaccagaaacatactctgaaataactactctggaaggtagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1321

Amino Acids

149.9

Weight (kDa)

6.04

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2465, 3752
AarI CACCTGC 1 cut(s) 503
Acc36I ACCTGC 1 cut(s) 503
AccB7I CCANNNNNTGG 1 cut(s) 637
AccI GTMKAC 1 cut(s) 536
AccII CGCG 2 cut(s) 1323, 2402
AciI CCGC 7 cut(s) 1321, 1451, 1751, 1925, 2610, 2960, 3612
AclWI GGATC 9 cut(s) 231, 1109, 1141, 1448, 1461, 2235, 3146, 3389, 3785
AcoI YGGCCR 3 cut(s) 733, 2702, 3459
AcuI CTGAAG 1 cut(s) 2619
AfaI GTAC 7 cut(s) 426, 2390, 2482, 2794, 3042, 3311, 3783
AfiI CCNNNNNNNGG 5 cut(s) 80, 637, 742, 2563, 3378
AflII CTTAAG 2 cut(s) 2044, 2392
AflIII ACRYGT 2 cut(s) 979, 3525
AhlI ACTAGT 1 cut(s) 188
AjiI CACGTC 1 cut(s) 483
AjuI GAANNNNNNNTTGG 2 cut(s) 3635, 3667
AleI CACNNNNGTG 1 cut(s) 825
Alw21I GWGCWC 4 cut(s) 369, 1803, 2281, 2363
Alw26I GTCTC 9 cut(s) 275, 489, 568, 824, 890, 2310, 2859, 3000, 3662
AlwI GGATC 9 cut(s) 231, 1109, 1141, 1448, 1461, 2235, 3146, 3389, 3785
Ama87I CYCGRG 1 cut(s) 2280
AoxI GGCC 4 cut(s) 733, 2702, 3122, 3459
ApeKI GCWGC 5 cut(s) 107, 956, 1902, 1905, 2962
ArsI GACNNNNNNTTYG 6 cut(s) 2055, 2087, 3558, 3590, 3640, 3672
AseI ATTAAT 2 cut(s) 1881, 2418
Asp700I GAANNNNTTC 2 cut(s) 231, 1375
AspLEI GCGC 2 cut(s) 1676, 1858
AspS9I GGNCC 4 cut(s) 2085, 2220, 2438, 3122
AsuHPI GGTGA 8 cut(s) 397, 812, 1371, 1451, 1792, 2068, 2141, 2267
AsuII TTCGAA 1 cut(s) 1578
AvaI CYCGRG 1 cut(s) 2280
AvaII GGWCC 3 cut(s) 2085, 2220, 2438
AxyI CCTNAGG 1 cut(s) 1692
BalI TGGCCA 3 cut(s) 735, 2704, 3461
BamHI GGATCC 1 cut(s) 1453
BanII GRGCYC 1 cut(s) 3774
BarI GAAGNNNNNNTAC 4 cut(s) 2793, 2825, 2914, 2946
BauI CACGAG 1 cut(s) 3001
Bbv12I GWGCWC 4 cut(s) 369, 1803, 2281, 2363
BbvI GCAGC 5 cut(s) 119, 943, 1892, 1914, 2974
BccI CCATC 9 cut(s) 493, 1061, 1124, 1625, 1991, 2438, 2641, 2671, 3542
BceAI ACGGC 1 cut(s) 2791
BciVI GTATCC 1 cut(s) 1426
BclI TGATCA 1 cut(s) 720
BcoDI GTCTC 9 cut(s) 275, 489, 568, 824, 890, 2310, 2859, 3000, 3662
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 8 cut(s) 189, 273, 1583, 2364, 2529, 3068, 3599, 3900
BfmI CTRYAG 3 cut(s) 2320, 2692, 3075
BfrI CTTAAG 2 cut(s) 2044, 2392
BfuAI ACCTGC 1 cut(s) 503
BfuI GTATCC 1 cut(s) 1426
BglI GCCNNNNNGGC 1 cut(s) 2331
BisI GCNGC 6 cut(s) 108, 957, 1751, 1903, 1906, 2963
BlsI GCNGC 6 cut(s) 109, 958, 1752, 1904, 1907, 2964
Bme18I GGWCC 3 cut(s) 2085, 2220, 2438
BmeT110I CYCGRG 1 cut(s) 2280
BmgBI CACGTC 1 cut(s) 483
BmgT120I GGNCC 4 cut(s) 2085, 2220, 2438, 3122
BmiI GGNNCC 5 cut(s) 927, 1418, 1455, 2658, 3514
BmrI ACTGGG 3 cut(s) 1181, 1572, 1954
BmsI GCATC 8 cut(s) 700, 943, 982, 1014, 1494, 1674, 1991, 2907
BmuI ACTGGG 3 cut(s) 1181, 1572, 1954
BoxI GACNNNNGTC 1 cut(s) 2014
BpmI CTGGAG 3 cut(s) 1311, 1815, 3168
Bpu14I TTCGAA 1 cut(s) 1578
BpuEI CTTGAG 1 cut(s) 2781
Bsa29I ATCGAT 1 cut(s) 1125
BsaBI GATNNNNATC 1 cut(s) 996
BsaJI CCNNGG 5 cut(s) 1499, 2125, 2223, 2434, 3486
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 637, 742, 2563, 3378
Bse21I CCTNAGG 1 cut(s) 1692
Bse3DI GCAATG 5 cut(s) 918, 966, 1915, 2413, 3415
Bse8I GATNNNNATC 1 cut(s) 996
BseCI ATCGAT 1 cut(s) 1125
BseDI CCNNGG 5 cut(s) 1499, 2125, 2223, 2434, 3486
BseGI GGATG 9 cut(s) 373, 751, 1072, 1509, 2006, 2194, 2621, 3427, 3553
BseJI GATNNNNATC 1 cut(s) 996
BseLI CCNNNNNNNGG 5 cut(s) 80, 637, 742, 2563, 3378
BseMI GCAATG 5 cut(s) 918, 966, 1915, 2413, 3415
BseMII CTCAG 5 cut(s) 1683, 1760, 2287, 2348, 3277
BseRI GAGGAG 3 cut(s) 695, 2261, 3440
BseXI GCAGC 5 cut(s) 119, 943, 1892, 1914, 2974
BsgI GTGCAG 1 cut(s) 2662
Bsh1236I CGCG 2 cut(s) 1323, 2402
BshFI GGCC 4 cut(s) 735, 2704, 3124, 3461
BshVI ATCGAT 1 cut(s) 1125
BsiHKAI GWGCWC 4 cut(s) 369, 1803, 2281, 2363
BsiHKCI CYCGRG 1 cut(s) 2280
BsiSI CCGG 1 cut(s) 3533
BslFI GGGAC 2 cut(s) 3204, 3690
BslI CCNNNNNNNGG 5 cut(s) 80, 637, 742, 2563, 3378
BsmAI GTCTC 9 cut(s) 275, 489, 568, 824, 890, 2310, 2859, 3000, 3662
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 3204, 3690
BsmI GAATGC 3 cut(s) 2114, 2122, 3628
BsnI GGCC 4 cut(s) 735, 2704, 3124, 3461
BsoBI CYCGRG 1 cut(s) 2280
Bsp119I TTCGAA 1 cut(s) 1578
Bsp1286I GDGCHC 5 cut(s) 369, 1803, 2281, 2363, 3774
BspACI CCGC 7 cut(s) 1321, 1451, 1751, 1925, 2610, 2960, 3612
BspANI GGCC 4 cut(s) 735, 2704, 3124, 3461
BspCNI CTCAG 5 cut(s) 1684, 1759, 2286, 2349, 3276
BspDI ATCGAT 1 cut(s) 1125
BspFNI CGCG 2 cut(s) 1323, 2402
BspLI GGNNCC 5 cut(s) 927, 1418, 1455, 2658, 3514
BspMAI CTGCAG 1 cut(s) 2696
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 9 cut(s) 231, 1109, 1141, 1448, 1461, 2235, 3146, 3389, 3785
BspQI GCTCTTC 2 cut(s) 357, 978
BspT104I TTCGAA 1 cut(s) 1578
BspTI CTTAAG 2 cut(s) 2044, 2392
BsrDI GCAATG 5 cut(s) 918, 966, 1915, 2413, 3415
BssECI CCNNGG 5 cut(s) 1499, 2125, 2223, 2434, 3486
BssNAI GTATAC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 3001
BssT1I CCWWGG 3 cut(s) 2125, 2223, 2434
Bst1107I GTATAC 1 cut(s) 537
Bst2BI CACGAG 1 cut(s) 3001
Bst6I CTCTTC 4 cut(s) 357, 514, 978, 3919
BstAFI CTTAAG 2 cut(s) 2044, 2392
BstAPI GCANNNNNTGC 2 cut(s) 17, 1024
BstBI TTCGAA 1 cut(s) 1578
BstC8I GCNNGC 4 cut(s) 510, 2122, 2404, 2896
BstF5I GGATG 9 cut(s) 373, 751, 1072, 1509, 2006, 2194, 2621, 3427, 3553
BstFNI CGCG 2 cut(s) 1323, 2402
BstHHI GCGC 2 cut(s) 1676, 1858
BstMAI GTCTC 9 cut(s) 275, 489, 568, 824, 890, 2310, 2859, 3000, 3662
BstMWI GCNNNNNNNGC 8 cut(s) 17, 412, 962, 1024, 1262, 2331, 2408, 3900
BstNSI RCATGY 5 cut(s) 406, 983, 2898, 3316, 3529
BstPAI GACNNNNGTC 1 cut(s) 2014
BstSFI CTRYAG 3 cut(s) 2320, 2692, 3075
BstUI CGCG 2 cut(s) 1323, 2402
BstV1I GCAGC 5 cut(s) 119, 943, 1892, 1914, 2974
BstX2I RGATCY 4 cut(s) 1453, 2227, 3138, 3790
BstYI RGATCY 4 cut(s) 1453, 2227, 3138, 3790
BstZ17I GTATAC 1 cut(s) 537
Bsu15I ATCGAT 1 cut(s) 1125
Bsu36I CCTNAGG 1 cut(s) 1692
BsuI GTATCC 1 cut(s) 1426
BsuRI GGCC 4 cut(s) 735, 2704, 3124, 3461
BsuTUI ATCGAT 1 cut(s) 1125
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 9 cut(s) 373, 751, 1072, 1509, 2006, 2194, 2621, 3427, 3553
BtsI GCAGTG 4 cut(s) 1841, 1879, 2175, 2202
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 4 cut(s) 510, 2122, 2404, 2896
CfoI GCGC 2 cut(s) 1676, 1858
Cfr13I GGNCC 4 cut(s) 2085, 2220, 2438, 3122
ClaI ATCGAT 1 cut(s) 1125
CseI GACGC 2 cut(s) 1676, 3080
CsiI ACCWGGT 2 cut(s) 289, 1419
Csp6I GTAC 7 cut(s) 425, 2389, 2481, 2793, 3041, 3310, 3782
CviQI GTAC 7 cut(s) 425, 2389, 2481, 2793, 3041, 3310, 3782
EaeI YGGCCR 3 cut(s) 733, 2702, 3459
Eam1104I CTCTTC 4 cut(s) 357, 514, 978, 3919
EarI CTCTTC 4 cut(s) 357, 514, 978, 3919
EciI GGCGGA 1 cut(s) 2599
Eco130I CCWWGG 3 cut(s) 2125, 2223, 2434
Eco24I GRGCYC 1 cut(s) 3774
Eco47I GGWCC 3 cut(s) 2085, 2220, 2438
Eco57I CTGAAG 1 cut(s) 2619
Eco81I CCTNAGG 1 cut(s) 1692
Eco88I CYCGRG 1 cut(s) 2280
EcoRI GAATTC 2 cut(s) 1726, 1786
EcoT14I CCWWGG 3 cut(s) 2125, 2223, 2434
EcoT22I ATGCAT 4 cut(s) 13, 997, 2900, 3544
EcoT38I GRGCYC 1 cut(s) 3774
ErhI CCWWGG 3 cut(s) 2125, 2223, 2434
Esp3I CGTCTC 1 cut(s) 489
FalI AAGNNNNNCTT 2 cut(s) 3125, 3157
FaqI GGGAC 2 cut(s) 3204, 3690
FauI CCCGC 1 cut(s) 1932
FauNDI CATATG 3 cut(s) 1020, 1214, 2021
FbaI TGATCA 1 cut(s) 720
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 6 cut(s) 108, 957, 1751, 1903, 1906, 2963
FokI GGATG 9 cut(s) 360, 758, 1079, 1516, 2013, 2201, 2608, 3434, 3560
FriOI GRGCYC 1 cut(s) 3774
Fsp4HI GCNGC 6 cut(s) 108, 957, 1751, 1903, 1906, 2963
FspBI CTAG 8 cut(s) 189, 273, 1583, 2364, 2529, 3068, 3599, 3900
GlaI GCGC 2 cut(s) 1675, 1857
GluI GCNGC 6 cut(s) 108, 957, 1751, 1903, 1906, 2963
GsuI CTGGAG 3 cut(s) 1311, 1815, 3168
HaeIII GGCC 4 cut(s) 735, 2704, 3124, 3461
HapII CCGG 1 cut(s) 3533
HgaI GACGC 2 cut(s) 1676, 3080
HhaI GCGC 2 cut(s) 1676, 1858
Hin6I GCGC 2 cut(s) 1674, 1856
HinP1I GCGC 2 cut(s) 1674, 1856
HincII GTYRAC 3 cut(s) 1359, 1446, 1530
HindII GTYRAC 3 cut(s) 1359, 1446, 1530
HindIII AAGCTT 2 cut(s) 583, 3325
HpaII CCGG 1 cut(s) 3533
HphI GGTGA 8 cut(s) 397, 812, 1371, 1451, 1792, 2068, 2141, 2267
Hpy166II GTNNAC 8 cut(s) 537, 1359, 1446, 1530, 1614, 2059, 2483, 3130
Hpy8I GTNNAC 8 cut(s) 537, 1359, 1446, 1530, 1614, 2059, 2483, 3130
Hpy99I CGWCG 1 cut(s) 1547
HpyCH4IV ACGT 1 cut(s) 482
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 412, 962, 1024, 1262, 2331, 2408, 3900
HpySE526I ACGT 1 cut(s) 482
HspAI GCGC 2 cut(s) 1674, 1856
Ksp22I TGATCA 1 cut(s) 720
LguI GCTCTTC 2 cut(s) 357, 978
LmnI GCTCC 4 cut(s) 682, 931, 1539, 2656
Lsp1109I GCAGC 5 cut(s) 119, 943, 1892, 1914, 2974
LweI GCATC 8 cut(s) 700, 943, 982, 1014, 1494, 1674, 1991, 2907
MabI ACCWGGT 2 cut(s) 289, 1419
MaeI CTAG 8 cut(s) 189, 273, 1583, 2364, 2529, 3068, 3599, 3900
MaeII ACGT 1 cut(s) 482
MfeI CAATTG 2 cut(s) 1302, 3090
MflI RGATCY 4 cut(s) 1453, 2227, 3138, 3790
MhlI GDGCHC 5 cut(s) 369, 1803, 2281, 2363, 3774
MlsI TGGCCA 3 cut(s) 735, 2704, 3461
MluNI TGGCCA 3 cut(s) 735, 2704, 3461
MlyI GAGTC 6 cut(s) 2079, 2258, 2991, 3255, 3672, 3853
MmeI TCCRAC 4 cut(s) 790, 1505, 3451, 3681
Mox20I TGGCCA 3 cut(s) 735, 2704, 3461
Mph1103I ATGCAT 4 cut(s) 13, 997, 2900, 3544
MroXI GAANNNNTTC 2 cut(s) 231, 1375
MscI TGGCCA 3 cut(s) 735, 2704, 3461
MslI CAYNNNNRTG 3 cut(s) 825, 883, 2054
Msp20I TGGCCA 3 cut(s) 735, 2704, 3461
MspA1I CMGCKG 4 cut(s) 1451, 1905, 1927, 3612
MspCI CTTAAG 2 cut(s) 2044, 2392
MspI CCGG 1 cut(s) 3533
MunI CAATTG 2 cut(s) 1302, 3090
Mva1269I GAATGC 3 cut(s) 2114, 2122, 3628
MvnI CGCG 2 cut(s) 1323, 2402
MwoI GCNNNNNNNGC 8 cut(s) 17, 412, 962, 1024, 1262, 2331, 2408, 3900
NdeI CATATG 3 cut(s) 1020, 1214, 2021
NlaIV GGNNCC 5 cut(s) 927, 1418, 1455, 2658, 3514
NmuCI GTSAC 3 cut(s) 478, 1228, 2255
NsiI ATGCAT 4 cut(s) 13, 997, 2900, 3544
NspI RCATGY 5 cut(s) 406, 983, 2898, 3316, 3529
NspV TTCGAA 1 cut(s) 1578
OliI CACNNNNGTG 1 cut(s) 825
PaeI GCATGC 1 cut(s) 2898
PaeR7I CTCGAG 1 cut(s) 2280
PaqCI CACCTGC 1 cut(s) 503
PciI ACATGT 2 cut(s) 979, 3525
PciSI GCTCTTC 2 cut(s) 357, 978
PctI GAATGC 3 cut(s) 2114, 2122, 3628
PdmI GAANNNNTTC 2 cut(s) 231, 1375
PflMI CCANNNNNTGG 1 cut(s) 637
PfoI TCCNGGA 1 cut(s) 3145
PkrI GCNGC 6 cut(s) 109, 958, 1752, 1904, 1907, 2964
PleI GAGTC 6 cut(s) 2078, 2257, 2990, 3255, 3672, 3853
PpsI GAGTC 6 cut(s) 2078, 2257, 2990, 3255, 3672, 3853
PscI ACATGT 2 cut(s) 979, 3525
PshAI GACNNNNGTC 1 cut(s) 2014
PshBI ATTAAT 2 cut(s) 1881, 2418
PsiI TTATAA 2 cut(s) 2465, 3752
PspN4I GGNNCC 5 cut(s) 927, 1418, 1455, 2658, 3514
PspPI GGNCC 4 cut(s) 2085, 2220, 2438, 3122
PspXI VCTCGAGB 1 cut(s) 2280
PstI CTGCAG 1 cut(s) 2696
PsuI RGATCY 4 cut(s) 1453, 2227, 3138, 3790
PvuII CAGCTG 1 cut(s) 1905
RsaI GTAC 7 cut(s) 426, 2390, 2482, 2794, 3042, 3311, 3783
RsaNI GTAC 7 cut(s) 425, 2389, 2481, 2793, 3041, 3310, 3782
RseI CAYNNNNRTG 3 cut(s) 825, 883, 2054
SapI GCTCTTC 2 cut(s) 357, 978
SatI GCNGC 6 cut(s) 108, 957, 1751, 1903, 1906, 2963
Sau96I GGNCC 4 cut(s) 2085, 2220, 2438, 3122
SchI GAGTC 6 cut(s) 2079, 2258, 2991, 3255, 3672, 3853
SduI GDGCHC 5 cut(s) 369, 1803, 2281, 2363, 3774
SexAI ACCWGGT 2 cut(s) 289, 1419
SfaNI GCATC 8 cut(s) 700, 943, 982, 1014, 1494, 1674, 1991, 2907
SfcI CTRYAG 3 cut(s) 2320, 2692, 3075
Sfr274I CTCGAG 1 cut(s) 2280
SfuI TTCGAA 1 cut(s) 1578
SinI GGWCC 3 cut(s) 2085, 2220, 2438
SlaI CTCGAG 1 cut(s) 2280
SmiMI CAYNNNNRTG 3 cut(s) 825, 883, 2054
SmlI CTYRAG 4 cut(s) 2044, 2280, 2392, 2796
SmoI CTYRAG 4 cut(s) 2044, 2280, 2392, 2796
SpeI ACTAGT 1 cut(s) 188
SphI GCATGC 1 cut(s) 2898
SsiI CCGC 7 cut(s) 1321, 1451, 1751, 1925, 2610, 2960, 3612
SspI AATATT 3 cut(s) 620, 939, 1667
SspMI CTAG 8 cut(s) 189, 273, 1583, 2364, 2529, 3068, 3599, 3900
StyI CCWWGG 3 cut(s) 2125, 2223, 2434
TaiI ACGT 1 cut(s) 485
TaqI TCGA 8 cut(s) 235, 790, 976, 1125, 1578, 2182, 2281, 2967
TaqII GACCGA 1 cut(s) 1569
TatI WGTACW 5 cut(s) 424, 2388, 2480, 2792, 3309
TauI GCSGC 1 cut(s) 1753
TseFI GTSAC 3 cut(s) 478, 1228, 2255
TseI GCWGC 5 cut(s) 107, 956, 1902, 1905, 2962
Tsp45I GTSAC 3 cut(s) 478, 1228, 2255
TspGWI ACGGA 2 cut(s) 433, 3901
Van91I CCANNNNNTGG 1 cut(s) 637
Vha464I CTTAAG 2 cut(s) 2044, 2392
VpaK11BI GGWCC 3 cut(s) 2085, 2220, 2438
VspI ATTAAT 2 cut(s) 1881, 2418
XbaI TCTAGA 3 cut(s) 272, 2528, 3598
XceI RCATGY 5 cut(s) 406, 983, 2898, 3316, 3529
XhoI CTCGAG 1 cut(s) 2280
XmiI GTMKAC 1 cut(s) 536
XmnI GAANNNNTTC 2 cut(s) 231, 1375
XspI CTAG 8 cut(s) 189, 273, 1583, 2364, 2529, 3068, 3599, 3900
Zsp2I ATGCAT 4 cut(s) 13, 997, 2900, 3544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.