Rh2BG566400

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000412
Physical Location & Seq
Reverse (-)
77986111 .. 77989774
3664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG566400.1

Sequence Viewer

Length: 897 bp
ATGATGAAAGTGGGAAACCCAGATTCCCAATTTAATGCCAAAAGTGAAATGGAATGCAGAGTAGAGTGCCTTAACAACTGTGAGTGTCAAGCTTATTTCTATGAAGAGGTGGAGAACAAAAACACGGGTGGGAGTAGTAGTTCAACATGTTGGATTTGGTCACAAGATGTCACCAATCTTCAGGAGGACTATGACGGTGGCCAGAATCTACAGGTTCGTGTAGCAGTTTCAGATATAGAATCAACAGCGAGAAGCTGTGGAAGTTGTGGCACAAACCTGATCCCTTATCCCTTAAGCACTGGACCAAAGTGTGGTGATGTCACCTACTATAGTTTCCACTGCAATATTTCAACTGGCCAGCTGAGCTTTGAGGCACCAAGCGGCACCTACCATGTCACAAGCATCAACGCAGACACACAAACATTTGTCATCCAAGCTAATGATGCAGATGAATGTAGAGATAAAAAATTTCTGAAGCTCATACAGTCTTCTCCATATAATGTGACAAACATGTGCAATGCTGATCCGACCCGTTTTAGTCCTGATTTGTCATTTAAAGGAGGATATGAAGTTGAAGTTGCTTGGGAGTCACCCTTGGAACCACCTTGTTCCTCATCTACAGACTGCAAGGACTGGCCTCGTTCGATATGTGATGCTGCTCTAGATGGGAAGAATAGGTGTCTTTGCCCTGCAAACTCAAAATGGGATAGCAGGAGTTTAAATTGTACTCAAGAAGTTGGCCACAGAAAGCAAACTGGTGAGCAAGGGAAGATGACCCTAGCTCTAATCATTGCAGTAACTTGTATAAGTGTAGCTGTTCTAGCAATTCTTTCAAGTACCTTTGTTTATGCTTATATATGGAGAAGAAGGCGCATTAAGACACAAGCTACACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

298

Amino Acids

32.99

Weight (kDa)

5.12

Isoelectric Point (pI)

55.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 7 - 59 3.3e-08 PAN-like domain
GUB_WAK_bind PF13947 87 - 144 5.1e-07 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 373, 383
AccB7I CCANNNNNTGG 1 cut(s) 311
AciI CCGC 1 cut(s) 381
AclWI GGATC 2 cut(s) 274, 518
AcoI YGGCCR 3 cut(s) 199, 355, 739
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 2 cut(s) 164, 494
AfaI GTAC 2 cut(s) 727, 838
AfiI CCNNNNNNNGG 1 cut(s) 311
AflII CTTAAG 1 cut(s) 292
AflIII ACRYGT 2 cut(s) 146, 510
AgsI TTSAA 4 cut(s) 144, 351, 575, 834
AloI GAACNNNNNNTCC 2 cut(s) 124, 156
AluBI AGCT 9 cut(s) 92, 255, 361, 366, 437, 478, 782, 815, 887
AluI AGCT 9 cut(s) 92, 255, 361, 366, 437, 478, 782, 815, 887
AlwI GGATC 2 cut(s) 274, 518
AoxI GGCC 4 cut(s) 199, 355, 635, 739
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 1 cut(s) 467
AspLEI GCGC 1 cut(s) 873
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 5 cut(s) 163, 313, 326, 582, 770
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 3 cut(s) 201, 357, 741
BanI GGYRCC 2 cut(s) 373, 383
BbsI GAAGAC 1 cut(s) 480
BbvI GCAGC 1 cut(s) 643
BccI CCATC 1 cut(s) 659
BfaI CTAG 3 cut(s) 662, 779, 821
BfmI CTRYAG 3 cut(s) 209, 328, 618
BfrI CTTAAG 1 cut(s) 292
BisI GCNGC 2 cut(s) 382, 657
BlpI GCTNAGC 1 cut(s) 362
BlsI GCNGC 2 cut(s) 383, 658
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 3 cut(s) 375, 385, 600
BmsI GCATC 3 cut(s) 411, 433, 643
BpiI GAAGAC 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 714
BsaJI CCNNGG 1 cut(s) 594
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse1I ACTGG 4 cut(s) 304, 358, 638, 760
Bse3DI GCAATG 2 cut(s) 523, 789
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMI GCAATG 2 cut(s) 523, 789
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 4 cut(s) 304, 358, 638, 760
BseXI GCAGC 1 cut(s) 643
BshFI GGCC 4 cut(s) 201, 357, 637, 741
BshNI GGYRCC 2 cut(s) 373, 383
BslI CCNNNNNNNGG 1 cut(s) 311
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 4 cut(s) 201, 357, 637, 741
Bsp143I GATC 2 cut(s) 279, 523
Bsp1720I GCTNAGC 1 cut(s) 362
BspACI CCGC 1 cut(s) 381
BspANI GGCC 4 cut(s) 201, 357, 637, 741
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 3 cut(s) 375, 385, 600
BspPI GGATC 2 cut(s) 274, 518
BspT107I GGYRCC 2 cut(s) 373, 383
BspTI CTTAAG 1 cut(s) 292
BsrDI GCAATG 2 cut(s) 523, 789
BsrI ACTGG 4 cut(s) 304, 358, 638, 760
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 2 cut(s) 279, 523
BssT1I CCWWGG 1 cut(s) 594
Bst4CI ACNGT 3 cut(s) 80, 197, 486
Bst6I CTCTTC 1 cut(s) 99
BstAFI CTTAAG 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 362
BstF5I GGATG 1 cut(s) 429
BstHHI GCGC 1 cut(s) 873
BstKTI GATC 2 cut(s) 282, 526
BstMBI GATC 2 cut(s) 279, 523
BstMWI GCNNNNNNNGC 3 cut(s) 363, 443, 821
BstNSI RCATGY 2 cut(s) 150, 514
BstSFI CTRYAG 3 cut(s) 209, 328, 618
BstV1I GCAGC 1 cut(s) 643
BstV2I GAAGAC 1 cut(s) 480
BsuRI GGCC 4 cut(s) 201, 357, 637, 741
BtsCI GGATG 1 cut(s) 429
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 297, 337
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 873
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 726, 837
CviAII CATG 3 cut(s) 147, 392, 511
CviQI GTAC 2 cut(s) 726, 837
DdeI CTNAG 1 cut(s) 362
DpnI GATC 2 cut(s) 281, 525
DpnII GATC 2 cut(s) 279, 523
DraI TTTAAA 2 cut(s) 556, 720
EaeI YGGCCR 3 cut(s) 199, 355, 739
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 594
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 2 cut(s) 164, 494
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 3 cut(s) 150, 395, 514
FatI CATG 3 cut(s) 146, 391, 510
Fnu4HI GCNGC 2 cut(s) 382, 657
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 2 cut(s) 382, 657
FspBI CTAG 3 cut(s) 662, 779, 821
GlaI GCGC 1 cut(s) 872
GluI GCNGC 2 cut(s) 382, 657
HaeIII GGCC 4 cut(s) 201, 357, 637, 741
HhaI GCGC 1 cut(s) 873
Hin1II CATG 3 cut(s) 150, 395, 514
Hin6I GCGC 1 cut(s) 871
HinP1I GCGC 1 cut(s) 871
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 4 cut(s) 23, 205, 239, 587
HphI GGTGA 5 cut(s) 163, 313, 326, 582, 770
Hpy188I TCNGA 3 cut(s) 232, 474, 528
Hpy188III TCNNGA 4 cut(s) 182, 542, 662, 731
HpyAV CCTTC 1 cut(s) 861
HpyCH4III ACNGT 3 cut(s) 80, 197, 486
HpyCH4V TGCA 7 cut(s) 57, 342, 446, 516, 627, 692, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 363, 443, 821
HpyF3I CTNAG 1 cut(s) 362
Hsp92II CATG 3 cut(s) 150, 395, 514
HspAI GCGC 1 cut(s) 871
Kzo9I GATC 2 cut(s) 279, 523
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 3 cut(s) 411, 433, 643
MaeI CTAG 3 cut(s) 662, 779, 821
MaeIII GTNAC 7 cut(s) 159, 169, 319, 394, 502, 588, 796
MalI GATC 2 cut(s) 281, 525
MboI GATC 2 cut(s) 279, 523
MboII GAAGA 6 cut(s) 116, 170, 480, 682, 781, 876
MlsI TGGCCA 3 cut(s) 201, 357, 741
MluCI AATT 4 cut(s) 29, 467, 721, 825
MluNI TGGCCA 3 cut(s) 201, 357, 741
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 2 cut(s) 131, 551
MnlI CCTC 6 cut(s) 100, 178, 364, 554, 622, 648
Mox20I TGGCCA 3 cut(s) 201, 357, 741
MscI TGGCCA 3 cut(s) 201, 357, 741
MseI TTAA 7 cut(s) 33, 72, 293, 555, 719, 876, 895
Msp20I TGGCCA 3 cut(s) 201, 357, 741
MspA1I CMGCKG 1 cut(s) 361
MspCI CTTAAG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 59
MwoI GCNNNNNNNGC 3 cut(s) 363, 443, 821
NdeII GATC 2 cut(s) 279, 523
NlaIII CATG 3 cut(s) 150, 395, 514
NlaIV GGNNCC 3 cut(s) 375, 385, 600
NmuCI GTSAC 6 cut(s) 159, 169, 319, 394, 502, 588
NspI RCATGY 2 cut(s) 150, 514
PciI ACATGT 2 cut(s) 146, 510
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 3 cut(s) 23, 205, 239
PflMI CCANNNNNTGG 1 cut(s) 311
PkrI GCNGC 2 cut(s) 383, 658
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PscI ACATGT 2 cut(s) 146, 510
PspN4I GGNNCC 3 cut(s) 375, 385, 600
PspPI GGNCC 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 361
RsaI GTAC 2 cut(s) 727, 838
RsaNI GTAC 2 cut(s) 726, 837
SaqAI TTAA 7 cut(s) 33, 72, 293, 555, 719, 876, 895
SatI GCNGC 2 cut(s) 382, 657
Sau3AI GATC 2 cut(s) 279, 523
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 596
SfaNI GCATC 3 cut(s) 411, 433, 643
SfcI CTRYAG 3 cut(s) 209, 328, 618
SinI GGWCC 1 cut(s) 302
SmlI CTYRAG 2 cut(s) 292, 729
SmoI CTYRAG 2 cut(s) 292, 729
Sse9I AATT 4 cut(s) 29, 467, 721, 825
SsiI CCGC 1 cut(s) 381
SspI AATATT 1 cut(s) 346
SspMI CTAG 3 cut(s) 662, 779, 821
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 3 cut(s) 80, 197, 486
TaqI TCGA 1 cut(s) 644
TasI AATT 4 cut(s) 29, 467, 721, 825
TatI WGTACW 1 cut(s) 725
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 3 cut(s) 23, 205, 239
Tru1I TTAA 7 cut(s) 33, 72, 293, 555, 719, 876, 895
Tru9I TTAA 7 cut(s) 33, 72, 293, 555, 719, 876, 895
TscAI CASTG 2 cut(s) 304, 344
TseFI GTSAC 6 cut(s) 159, 169, 319, 394, 502, 588
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 6 cut(s) 159, 169, 319, 394, 502, 588
TspDTI ATGAA 4 cut(s) 20, 117, 465, 582
TspRI CASTG 2 cut(s) 304, 344
Van91I CCANNNNNTGG 1 cut(s) 311
Vha464I CTTAAG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 467
XbaI TCTAGA 1 cut(s) 661
XceI RCATGY 2 cut(s) 150, 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 3 cut(s) 662, 779, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.