RLG00000021249

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000412
Physical Location & Seq
Reverse (-)
73343055 .. 73346856
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021249

Sequence Viewer

Length: 2595 bp
ATGTTCACTAACTTCTTTCTGTATTCATTCTTGTTATGCACTTTTCTTCATTGTTGCATTGCTAAAGATACAATAGCATATGACAGTTTGATTAGTGATGACAAAGGTCATACTATTGTTTCATCAGGAGAAAAATTTGAACTTGGGTTCTTTACTCCTAATGGAAGCTCTGGTACTAGAAGATATGTTGGAATTTGGTATTACAGATCGAATCCACAGACTGTTGTGTGGCTTGCAAATAGAGACAACCCTCTTTCAGATACTAGTGGAGTTTTCACTATAGCAGAAGATGGAAATCTTAAGGTGCTGGATGGCAAAAGAAAAACTTACTGGTCATCAAGTCTTGAAAGTTCATCATCAACAATTAGGACAGCAAAGCTCATGGACACTGGTAATCTTGTTGTGAGCAATAGAGAGCAAGGAAACAACTCAGTTCAAATTGTCTGGCAGAGTTTTGAAAATCCAACCGACACATTTCTTCCTGGCATGAAAATGAGCGGAAAATTAGCACTGAGTTCATGGAAGAGTTACAATGATCCAGCAACAGGAAATTTCACATTCCAGCAAGACCAAGAGGATGCAAACCACTTTGTTATCTGGAAAAAATCGAGAAGGTACTGGAAGAGTGAAGATTATGGCAACTTCATTAGTTCTGATGAGATGGCTTCTGCAATTCTCTACTTGCTATCAAACTTCACCTCTACAGCAGTCCACAATGACTCTGTGCCATATCTCACCTCCTCATTATACACTTCTACAAGGCTGGTTATGAGTTTTTCGGGCCAGATTCAGTATTTGATGTGGGATAGTGAGAAGGTTTGGTCTTTGATATGGGCAGATCCTAGAGATAGATGCAGTGTGTATAATGCATGTGGAAATTTTGGTAGCTGTAACAGCAAAAATGGTTTGGTTTGCAAGTGTTTGCCTGGTTTTAAGCCTAGTTCACCGGATAATTGGAATCATGGAGATTATTCAGGCGGATGCACTAGGAAGTCAACATTATGTGGCAACAATGCTGAGAGTTACACATTCTTGAGCTTAAAGATGATGAAAGTGGGAAACCCAGATTCCCAATTTAATGCCAAAAGTGAAGTGGAATGCAGAGTAGAGTGCCTTAACAACTGTGAGTGTCAAGCTTATTTCTTTGAAGAGGTGGAGAACAAAAAAACGGGTGGGAGTAGTAGTTCAACATGTTGGATTTGGTCACAAGATGTCACCAATCTTCAGGAGGACTATGACGGTGGCCAGAATCTACAGGTTCGTGTAGCAGTTTCAGATATAGAATCAACAGCGAGGAGCTGTGGAAGTTGTGGCACAAACCTGATCCCTTATCCCTTAAGCACTGGACCAAAGTGTGGTGATGTCACCTACTATAGTTTCCACTGCAATATTTCAACTGGCCAGCTGAGCTTTGAGGCACCAAGCGGCACCTACCATGTCACAAGCATCAACGCGGACACACAAACATTTGTCATCCAAGCTAATGATGCAGATGAATGTAGAGATAAAAAATTTCTGAAGCTCATCCAGTCTTCTCCATATAATGTGACAAACATGTGCAATGCTGATCCGACCCGTTTTAGTCCTGACTTGTCATTTAAAGGAGGATATGAAGTTGAAGTTGCTTGGGAGTCACCCTTGGAACCACCTTGTTCCTCATCTACAGACTGCAAGGACTGGCCTCGGTCGATATGTGATGCTGCTCTAGATGGGAAGAATAGGTGTCTTTGCCCTGCAAACTCAAAATGGGATAGCAGGAGTTTAAATTGTACTCAAGAAGTTGGCCGCAGAAAGCAAACTGGTGAGCAAGGGAAGATGACCCTAGCTCTAATCATTGCAGTAACTTGTATAAGTGTAGCTGTTCTAGCAATTCTTTCAAGTACCTTTGTTTATGCTTATATATGGAGAAGAAGGCGCATTAAGACACAAGAAGGCAGGGCAAATCTTCAAAAGTGTTCAACACTTAATCACTTTTATGACAGTGAGAGAAAAGTCAAGAACTTGATTGAATCAGGCCGATTTAAGGATGACGATACGGAGGGCATTGATGTACCCTCTTTTGATTTGGAAAGCATACTGGTAGCTACAACATACTTCTCTAATGCAAATAAACTTGGACAAGGAGGATTTGGTCCTGTTTACAAGGGTAAGCTTCCAGGAGGAGAAGAAATTGCTGTAAAGAGGCTCTCGAGTTGTTCAGGCCAAGGCTTAGAGGAATTCAAAAATGAAGTTTTGTTAATTGCCAAACTCCAACATCGGAATCTGGTTCGACTTTTGGGCTATTGTGCTGAGGGAGATGAAAAGATGTTAATCTATGAATACATGGCCAACAAAAGCTTAGACTCTTTCATCTTTGATCGAAAAGTATGTGTATCGTTGGACTGGAATACACGCTTTAACATCATCTTAGGAATTGCTCGGGGGCTTCTTTATCTTCACCAAGATTCTAGATTAAGGGTTATTCATAGAGATCTGAAAACCAGCAACATTCTACTGAGTGAAGAGATGAACCCCAAAATATCAGACTTCGGTTTGGCAAGGATCTTTGGAGGCAATGAAACTTCTGCAAACACCAATAGAGTAGTGGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

865

Amino Acids

96.61

Weight (kDa)

6.21

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 168 2.7e-30 D-mannose binding lectin
S_locus_glycop PF00954 214 - 314 1.5e-22 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 1.8e-08 PAN-like domain
GUB_WAK_bind PF13947 435 - 492 2.4e-06 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 700 - 864 5.1e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 702 - 852 4.2e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1417, 1427
AccB7I CCANNNNNTGG 1 cut(s) 1355
AccBSI CCGCTC 1 cut(s) 498
AccII CGCG 1 cut(s) 1454
AciI CCGC 5 cut(s) 498, 978, 1425, 1454, 1786
AclWI GGATC 5 cut(s) 530, 833, 1318, 1562, 2549
AcoI YGGCCR 4 cut(s) 1243, 1399, 1783, 2325
AcsI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2216
AcuI CTGAAG 2 cut(s) 1208, 1538
AfaI GTAC 5 cut(s) 175, 617, 1771, 1882, 2052
AfiI CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
AflII CTTAAG 2 cut(s) 299, 1336
AflIII ACRYGT 2 cut(s) 1190, 1554
AhlI ACTAGT 1 cut(s) 263
AjnI CCWGG 3 cut(s) 481, 925, 2155
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 5 cut(s) 530, 833, 1318, 1562, 2549
Ama87I CYCGRG 2 cut(s) 2188, 2418
AoxI GGCC 8 cut(s) 781, 1243, 1399, 1679, 1783, 2014, 2200, 2325
ApeKI GCWGC 1 cut(s) 1700
ApoI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2216
AspLEI GCGC 1 cut(s) 1917
AspS9I GGNCC 3 cut(s) 781, 1346, 2132
AsuHPI GGTGA 9 cut(s) 688, 727, 936, 1207, 1357, 1370, 1626, 1814, 2429
AvaI CYCGRG 2 cut(s) 2188, 2418
AvaII GGWCC 2 cut(s) 1346, 2132
BalI TGGCCA 3 cut(s) 1245, 1401, 2327
BanI GGYRCC 2 cut(s) 1417, 1427
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BbsI GAAGAC 1 cut(s) 1524
BbvCI CCTCAGC 1 cut(s) 2289
BbvI GCAGC 1 cut(s) 1687
BccI CCATC 4 cut(s) 284, 305, 655, 1703
BciT130I CCWGG 3 cut(s) 483, 927, 2157
BcoDI GTCTC 1 cut(s) 237
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 9 cut(s) 177, 264, 843, 939, 987, 1706, 1823, 1865, 2448
BfmI CTRYAG 5 cut(s) 279, 702, 1253, 1372, 1662
BfrI CTTAAG 2 cut(s) 299, 1336
BglII AGATCT 1 cut(s) 2470
BisI GCNGC 3 cut(s) 1426, 1701, 1786
BlpI GCTNAGC 1 cut(s) 1406
BlsI GCNGC 3 cut(s) 1427, 1702, 1787
Bme1390I CCNGG 3 cut(s) 483, 927, 2157
Bme18I GGWCC 2 cut(s) 1346, 2132
BmeT110I CYCGRG 2 cut(s) 2188, 2418
BmgT120I GGNCC 3 cut(s) 781, 1346, 2132
BmiI GGNNCC 3 cut(s) 1419, 1429, 1644
BmrFI CCNGG 3 cut(s) 483, 927, 2157
BmsI GCATC 6 cut(s) 568, 842, 971, 1455, 1477, 1687
BoxI GACNNNNGTC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 1524
Bpu10I CCTNAGC 1 cut(s) 2289
Bpu1102I GCTNAGC 1 cut(s) 1406
BpuEI CTTGAG 2 cut(s) 1054, 1758
BsaBI GATNNNNATC 2 cut(s) 294, 2309
BsaJI CCNNGG 3 cut(s) 1638, 1682, 2203
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 1168, 1198
Bsc4I CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
Bse3DI GCAATG 4 cut(s) 57, 1567, 1833, 2560
Bse8I GATNNNNATC 2 cut(s) 294, 2309
BseBI CCWGG 3 cut(s) 483, 927, 2157
BseDI CCNNGG 3 cut(s) 1638, 1682, 2203
BseGI GGATG 6 cut(s) 316, 583, 986, 1473, 1524, 2032
BseJI GATNNNNATC 2 cut(s) 294, 2309
BseLI CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
BseMI GCAATG 4 cut(s) 57, 1567, 1833, 2560
BseMII CTCAG 6 cut(s) 444, 503, 1008, 1397, 2280, 2486
BseRI GAGGAG 3 cut(s) 730, 1309, 2175
BseXI GCAGC 1 cut(s) 1687
Bsh1236I CGCG 1 cut(s) 1454
Bsh1285I CGRYCG 1 cut(s) 1688
BshFI GGCC 8 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327
BshNI GGYRCC 2 cut(s) 1417, 1427
BsiEI CGRYCG 1 cut(s) 1688
BsiHKCI CYCGRG 2 cut(s) 2188, 2418
BsiSI CCGG 1 cut(s) 947
BslI CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
BsmAI GTCTC 1 cut(s) 237
BsmI GAATGC 1 cut(s) 1103
BsnI GGCC 8 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327
BsoBI CYCGRG 2 cut(s) 2188, 2418
Bsp143I GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
Bsp1720I GCTNAGC 1 cut(s) 1406
BspACI CCGC 5 cut(s) 498, 978, 1425, 1454, 1786
BspANI GGCC 8 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327
BspCNI CTCAG 6 cut(s) 443, 504, 1009, 1398, 2281, 2487
BspFNI CGCG 1 cut(s) 1454
BspLI GGNNCC 3 cut(s) 1419, 1429, 1644
BspPI GGATC 5 cut(s) 530, 833, 1318, 1562, 2549
BspT107I GGYRCC 2 cut(s) 1417, 1427
BspTI CTTAAG 2 cut(s) 299, 1336
BsrBI CCGCTC 1 cut(s) 498
BsrDI GCAATG 4 cut(s) 57, 1567, 1833, 2560
BssECI CCNNGG 3 cut(s) 1638, 1682, 2203
BssMI GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
BssT1I CCWWGG 2 cut(s) 1638, 2203
Bst2UI CCWGG 3 cut(s) 483, 927, 2157
Bst4CI ACNGT 5 cut(s) 86, 223, 1124, 1241, 1982
Bst6I CTCTTC 4 cut(s) 518, 617, 1143, 2496
BstAFI CTTAAG 2 cut(s) 299, 1336
BstC8I GCNNGC 2 cut(s) 234, 1403
BstDEI CTNAG 9 cut(s) 430, 512, 1017, 1406, 2209, 2289, 2338, 2407, 2495
BstF5I GGATG 6 cut(s) 316, 583, 986, 1473, 1524, 2032
BstFNI CGCG 1 cut(s) 1454
BstHHI GCGC 1 cut(s) 1917
BstKTI GATC 8 cut(s) 209, 538, 841, 1326, 1570, 2359, 2473, 2544
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
BstMCI CGRYCG 1 cut(s) 1688
BstMWI GCNNNNNNNGC 4 cut(s) 894, 1407, 1487, 1865
BstNI CCWGG 3 cut(s) 483, 927, 2157
BstNSI RCATGY 3 cut(s) 873, 1194, 1558
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 3 cut(s) 481, 925, 2155
BstSFI CTRYAG 5 cut(s) 279, 702, 1253, 1372, 1662
BstUI CGCG 1 cut(s) 1454
BstV1I GCAGC 1 cut(s) 1687
BstV2I GAAGAC 1 cut(s) 1524
BstX2I RGATCY 3 cut(s) 838, 2470, 2541
BstYI RGATCY 3 cut(s) 838, 2470, 2541
BsuRI GGCC 8 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327
BtsCI GGATG 6 cut(s) 316, 583, 986, 1473, 1524, 2032
BtsI GCAGTG 2 cut(s) 862, 1381
BtsIMutI CAGTG 6 cut(s) 387, 509, 862, 1341, 1381, 1987
Cac8I GCNNGC 2 cut(s) 234, 1403
CfoI GCGC 1 cut(s) 1917
Cfr13I GGNCC 3 cut(s) 781, 1346, 2132
Csp6I GTAC 5 cut(s) 174, 616, 1770, 1881, 2051
CviAII CATG 9 cut(s) 382, 487, 519, 870, 962, 1191, 1436, 1555, 2323
CviQI GTAC 5 cut(s) 174, 616, 1770, 1881, 2051
DdeI CTNAG 9 cut(s) 430, 512, 1017, 1406, 2209, 2289, 2338, 2407, 2495
DpnI GATC 8 cut(s) 208, 537, 840, 1325, 1569, 2358, 2472, 2543
DpnII GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
DraI TTTAAA 2 cut(s) 1600, 1764
EaeI YGGCCR 4 cut(s) 1243, 1399, 1783, 2325
Eam1104I CTCTTC 4 cut(s) 518, 617, 1143, 2496
EarI CTCTTC 4 cut(s) 518, 617, 1143, 2496
EciI GGCGGA 1 cut(s) 993
Eco130I CCWWGG 2 cut(s) 1638, 2203
Eco47I GGWCC 2 cut(s) 1346, 2132
Eco57I CTGAAG 2 cut(s) 1208, 1538
Eco88I CYCGRG 2 cut(s) 2188, 2418
EcoRI GAATTC 1 cut(s) 2216
EcoRII CCWGG 3 cut(s) 481, 925, 2155
EcoT14I CCWWGG 2 cut(s) 1638, 2203
EcoT22I ATGCAT 1 cut(s) 871
ErhI CCWWGG 2 cut(s) 1638, 2203
FaeI CATG 9 cut(s) 385, 490, 522, 873, 965, 1194, 1439, 1558, 2326
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FatI CATG 9 cut(s) 381, 486, 518, 869, 961, 1190, 1435, 1554, 2322
FauNDI CATATG 1 cut(s) 79
Fnu4HI GCNGC 3 cut(s) 1426, 1701, 1786
FokI GGATG 6 cut(s) 323, 590, 993, 1460, 1511, 2039
Fsp4HI GCNGC 3 cut(s) 1426, 1701, 1786
FspBI CTAG 9 cut(s) 177, 264, 843, 939, 987, 1706, 1823, 1865, 2448
GlaI GCGC 1 cut(s) 1916
GluI GCNGC 3 cut(s) 1426, 1701, 1786
HaeIII GGCC 8 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327
HapII CCGG 1 cut(s) 947
HhaI GCGC 1 cut(s) 1917
Hin1II CATG 9 cut(s) 385, 490, 522, 873, 965, 1194, 1439, 1558, 2326
Hin6I GCGC 1 cut(s) 1915
HinP1I GCGC 1 cut(s) 1915
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HindIII AAGCTT 3 cut(s) 1134, 2150, 2335
HpaII CCGG 1 cut(s) 947
HphI GGTGA 9 cut(s) 688, 727, 936, 1207, 1357, 1370, 1626, 1814, 2429
Hpy166II GTNNAC 5 cut(s) 6, 712, 944, 996, 2140
Hpy188I TCNGA 8 cut(s) 259, 655, 1276, 1518, 1572, 2259, 2475, 2524
Hpy8I GTNNAC 5 cut(s) 6, 712, 944, 996, 2140
HpyAV CCTTC 4 cut(s) 606, 808, 1905, 1925
HpyCH4III ACNGT 5 cut(s) 86, 223, 1124, 1241, 1982
HpyF10VI GCNNNNNNNGC 4 cut(s) 894, 1407, 1487, 1865
HpyF3I CTNAG 9 cut(s) 430, 512, 1017, 1406, 2209, 2289, 2338, 2407, 2495
Hsp92II CATG 9 cut(s) 385, 490, 522, 873, 965, 1194, 1439, 1558, 2326
HspAI GCGC 1 cut(s) 1915
Kzo9I GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
LmnI GCTCC 1 cut(s) 1296
Lsp1109I GCAGC 1 cut(s) 1687
LweI GCATC 6 cut(s) 568, 842, 971, 1455, 1477, 1687
MaeI CTAG 9 cut(s) 177, 264, 843, 939, 987, 1706, 1823, 1865, 2448
MalI GATC 8 cut(s) 208, 537, 840, 1325, 1569, 2358, 2472, 2543
MbiI CCGCTC 1 cut(s) 498
MboI GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
MflI RGATCY 3 cut(s) 838, 2470, 2541
MlsI TGGCCA 3 cut(s) 1245, 1401, 2327
MluNI TGGCCA 3 cut(s) 1245, 1401, 2327
MlyI GAGTC 3 cut(s) 713, 1640, 2336
MmeI TCCRAC 6 cut(s) 169, 488, 1175, 1595, 2275, 2358
Mox20I TGGCCA 3 cut(s) 1245, 1401, 2327
Mph1103I ATGCAT 1 cut(s) 871
MscI TGGCCA 3 cut(s) 1245, 1401, 2327
MslI CAYNNNNRTG 2 cut(s) 491, 1974
Msp20I TGGCCA 3 cut(s) 1245, 1401, 2327
MspA1I CMGCKG 1 cut(s) 1405
MspCI CTTAAG 2 cut(s) 299, 1336
MspI CCGG 1 cut(s) 947
MspR9I CCNGG 3 cut(s) 483, 927, 2157
Mva1269I GAATGC 1 cut(s) 1103
MvaI CCWGG 3 cut(s) 483, 927, 2157
MvnI CGCG 1 cut(s) 1454
MwoI GCNNNNNNNGC 4 cut(s) 894, 1407, 1487, 1865
NdeI CATATG 1 cut(s) 79
NdeII GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
NlaIII CATG 9 cut(s) 385, 490, 522, 873, 965, 1194, 1439, 1558, 2326
NlaIV GGNNCC 3 cut(s) 1419, 1429, 1644
NmuCI GTSAC 6 cut(s) 1203, 1213, 1363, 1438, 1546, 1632
NsiI ATGCAT 1 cut(s) 871
NspI RCATGY 3 cut(s) 873, 1194, 1558
PaeR7I CTCGAG 1 cut(s) 2188
PciI ACATGT 2 cut(s) 1190, 1554
PctI GAATGC 1 cut(s) 1103
PfeI GAWTC 9 cut(s) 211, 787, 958, 1067, 1249, 1283, 2009, 2260, 2444
PflMI CCANNNNNTGG 1 cut(s) 1355
PfoI TCCNGGA 1 cut(s) 2155
PkrI GCNGC 3 cut(s) 1427, 1702, 1787
PleI GAGTC 3 cut(s) 713, 1639, 2336
PpsI GAGTC 3 cut(s) 713, 1639, 2336
PscI ACATGT 2 cut(s) 1190, 1554
PshAI GACNNNNGTC 1 cut(s) 105
Psp6I CCWGG 3 cut(s) 481, 925, 2155
PspGI CCWGG 3 cut(s) 481, 925, 2155
PspN4I GGNNCC 3 cut(s) 1419, 1429, 1644
PspPI GGNCC 3 cut(s) 781, 1346, 2132
PsuI RGATCY 3 cut(s) 838, 2470, 2541
PvuII CAGCTG 1 cut(s) 1405
RsaI GTAC 5 cut(s) 175, 617, 1771, 1882, 2052
RsaNI GTAC 5 cut(s) 174, 616, 1770, 1881, 2051
RseI CAYNNNNRTG 2 cut(s) 491, 1974
SatI GCNGC 3 cut(s) 1426, 1701, 1786
Sau3AI GATC 8 cut(s) 206, 535, 838, 1323, 1567, 2356, 2470, 2541
Sau96I GGNCC 3 cut(s) 781, 1346, 2132
SchI GAGTC 3 cut(s) 713, 1640, 2336
ScrFI CCNGG 3 cut(s) 483, 927, 2157
SfaNI GCATC 6 cut(s) 568, 842, 971, 1455, 1477, 1687
SfcI CTRYAG 5 cut(s) 279, 702, 1253, 1372, 1662
Sfr274I CTCGAG 1 cut(s) 2188
SinI GGWCC 2 cut(s) 1346, 2132
SlaI CTCGAG 1 cut(s) 2188
SmiMI CAYNNNNRTG 2 cut(s) 491, 1974
SmlI CTYRAG 5 cut(s) 299, 1033, 1336, 1773, 2188
SmoI CTYRAG 5 cut(s) 299, 1033, 1336, 1773, 2188
SpeI ACTAGT 1 cut(s) 263
SsiI CCGC 5 cut(s) 498, 978, 1425, 1454, 1786
SspI AATATT 1 cut(s) 1390
SspMI CTAG 9 cut(s) 177, 264, 843, 939, 987, 1706, 1823, 1865, 2448
StyD4I CCNGG 3 cut(s) 481, 925, 2155
StyI CCWWGG 2 cut(s) 1638, 2203
TaaI ACNGT 5 cut(s) 86, 223, 1124, 1241, 1982
TaqI TCGA 6 cut(s) 209, 608, 1688, 2189, 2269, 2359
TaqII GACCGA 1 cut(s) 1674
TatI WGTACW 1 cut(s) 1769
TauI GCSGC 2 cut(s) 1428, 1788
TfiI GAWTC 9 cut(s) 211, 787, 958, 1067, 1249, 1283, 2009, 2260, 2444
TscAI CASTG 6 cut(s) 394, 516, 862, 1348, 1388, 1987
TseFI GTSAC 6 cut(s) 1203, 1213, 1363, 1438, 1546, 1632
TseI GCWGC 1 cut(s) 1700
Tsp45I GTSAC 6 cut(s) 1203, 1213, 1363, 1438, 1546, 1632
TspGWI ACGGA 1 cut(s) 2051
TspRI CASTG 6 cut(s) 394, 516, 862, 1348, 1388, 1987
Van91I CCANNNNNTGG 1 cut(s) 1355
Vha464I CTTAAG 2 cut(s) 299, 1336
VpaK11BI GGWCC 2 cut(s) 1346, 2132
XapI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2216
XbaI TCTAGA 2 cut(s) 1705, 2447
XceI RCATGY 3 cut(s) 873, 1194, 1558
XcmI CCANNNNNNNNNTGG 2 cut(s) 1090, 2581
XhoI CTCGAG 1 cut(s) 2188
XspI CTAG 9 cut(s) 177, 264, 843, 939, 987, 1706, 1823, 1865, 2448
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.