Rh4AG077200

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000412
Physical Location & Seq
Reverse (-)
15642969 .. 15661463
18495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG077200.1

Sequence Viewer

Length: 2091 bp
ATGGTATCTTACGTCTTTTTCTTGTATGTAGTACTATTGCTGTGCGCTTATGACAACCTATTTTGTTCTGCTAGAGATACACTAACATCTGACGATGCAGTTATCGATGATGGATCAGAAGCTCTTGTTTCAGCAGGAGGTGAGTTTGAACTGGGTTTCTTCTCCACTACTACGGGGAAAAGATATGTGGGCATATGGTATCATAAGGTCACTCGAAGAACAGTTGTATGGGTTGCCAACAGAGCTGAACCTCTTCCTGCAGATTCCACTGGAGTTCTTACAATTGAAGAAGGGAATCTCCGCGTGTTTGATAATGTTACTGGGAAGCGTTATTGGTCAACAGAGATTGGAAAATCTTCACCTTTTAACAAGACAGTGAAGATAATGGATTCAGGGAACCTGGTGTTAAGTGATACTGGTGAGTTGACAGCGGTGATTTTGTGGCAGAGCTTTCAAAATCCAACTGATACATTCATTCCTGGGATGCGAATGGATAGTAACTTGAAGTTGACTTCATGGAGCGACGAAGATGACCGAAGAACTGGGAACTACACCTTCGAACTAGATCAAGAAGGGAAGTCAGAGAACCAGTTTACCATCTTAAATAGTTCGTTTCGTTACTGGAAAAGTGGAGAGCCAGGTAAATATTTAAGCTCAGATGAGATGCGTCCTGAGGTAGCTTACTTGTTATCAAATTTCAGCAAGAATTCTTCTCTTTATACAACTCAGCCTCAATTTGATTACAATTATACAAGATTGGTGATGAATTCTACTGGAGTGCTCAATGGTTTGACCTGGAATGACACCATTAAGCAGTGGGTTGTGTTTTTCTCTGCGCCAAAAGATCAATGCAGTGTGATTAATGCTTGTGGAAACTTTGGCAGCTGCAACATTAATAATACCCCGCTGGTGTGCAAGTGTTTGCCAGGTTTCAACCCCCAGTTTTTAGAGAAATGGAAATATGGAGATTTTTCAGATGGATGCAAAAGACAGTCGTCCATATGTGGCATGAAAGATACCTTCTTAAGCATAAAGGTGAACAAAGTGGGAATCTGGGAGTCATCGACCAATGTTACCAGTGATACAGAATGCGAGAAAGCGTGCCTTGGTAATTGCAAGTGCCAGGCTTACTCATCTGATTCATCTGTAAAGGCCACTGTTCGAAGGGATGATCTGAATATCACTGCTTGTTATCAATGGTCCACGGATCTAAATGATCTTGTAGAGGAGTCTGGTGACAATGGCAAGATTCTCAATGCTCGAGTTCCCGTGCCTGATTTAGAATCAACTACAAGAGACTGTAGCCCTTGTGGCACAACTACAATTCCTTATCCTCTGAGCACTACTGGAACAGATTGTGGCGACCCCATGTACAAAAGTTTCGCGTGCAATGCCTCCATTAATGAATTTAGCTTCCTTGGACCAGATGGCAAATCCTTTAGAGTTATAAATATCAGCCGAAGTACACAACGATTCATTATCAAATCCAAAAATATGAATTATTGTGATTCTAGAAACAGAGTGCTGAACATTTCCTTCCCGTTTAAGGTAATCAGTTGGTGTAGTTCTGACCAGATGAGGAAATTTATTTCTGGCATAGATTCATCCAGCAGTCCTTCAGTAATAGAGATGGGTTGGGAGCCACCATCGGAAGCAAACTGCACTATCTTAGACTGCAGGGATTGGCCATATTCAACTTGCAAAACAGTAAGAGGGGGAGAGAAAAAATGCCTTTGCAATACAAATTTTCAATGGAACGGCATTGACTTAAATTGTACTCAAGAAGGAAATCTACAAGAGCTATCAAATAAGAGTTCAACGAAAAAATTGTTATTGTCGCTGGTTGTCGTGGCAGTCATAAGTGGGATTTTTCTGGCATGCATCATTGGTTTTTGGATATGGAGAAGAAAGATTACAAACAGACGAGTCAATGAGGAGCCTGAGAGGGACACAATAACCTTTGCTTTATTACCTGCATTGTTCCCGATATATACATTGCTATGGAGTGTTCCGGCGCTTCTACCTTACACAAGGAACTATCATGGAACCCAAATTGGGGTCTATTATTTTGCTTGTTTGTCATATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

696

Amino Acids

78.21

Weight (kDa)

5.56

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 169 1.1e-24 D-mannose binding lectin
S_locus_glycop PF00954 214 - 314 4.2e-22 S-locus glycoprotein domain
PAN_2 PF08276 335 - 406 2.1e-07 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1448
Acc36I ACCTGC 1 cut(s) 1981
AccII CGCG 2 cut(s) 303, 1385
AciI CCGC 3 cut(s) 301, 431, 905
AclWI GGATC 2 cut(s) 121, 1215
AcoI YGGCCR 1 cut(s) 1685
AcsI RAATTY 6 cut(s) 694, 706, 766, 1406, 1583, 1744
AcuI CTGAAG 1 cut(s) 1602
AfaI GTAC 4 cut(s) 33, 1373, 1465, 1777
AfiI CCNNNNNNNGG 3 cut(s) 1546, 2055, 2056
AflII CTTAAG 1 cut(s) 1024
AgsI TTSAA 8 cut(s) 149, 287, 455, 505, 934, 1695, 1751, 1818
AjnI CCWGG 6 cut(s) 399, 478, 637, 794, 925, 1122
AluBI AGCT 8 cut(s) 122, 245, 450, 654, 680, 885, 1413, 1801
AluI AGCT 8 cut(s) 122, 245, 450, 654, 680, 885, 1413, 1801
Alw21I GWGCWC 2 cut(s) 783, 1343
Alw26I GTCTC 1 cut(s) 1290
AlwI GGATC 2 cut(s) 121, 1215
Ama87I CYCGRG 1 cut(s) 1260
AoxI GGCC 2 cut(s) 1152, 1685
ApeKI GCWGC 2 cut(s) 882, 885
ApoI RAATTY 6 cut(s) 694, 706, 766, 1406, 1583, 1744
AseI ATTAAT 3 cut(s) 861, 894, 1401
Asp700I GAANNNNTTC 2 cut(s) 252, 355
AspLEI GCGC 3 cut(s) 47, 838, 2017
AspS9I GGNCC 2 cut(s) 1200, 1421
AsuHPI GGTGA 7 cut(s) 152, 351, 431, 445, 772, 1048, 1247
AsuII TTCGAA 2 cut(s) 558, 1162
AvaI CYCGRG 1 cut(s) 1260
AvaII GGWCC 2 cut(s) 1200, 1421
AxyI CCTNAGG 1 cut(s) 672
BalI TGGCCA 1 cut(s) 1687
BarI GAAGNNNNNNTAC 4 cut(s) 1776, 1808, 1897, 1929
Bbv12I GWGCWC 2 cut(s) 783, 1343
BbvI GCAGC 2 cut(s) 872, 894
BccI CCATC 6 cut(s) 104, 605, 971, 1421, 1624, 1654
BceAI ACGGC 1 cut(s) 1774
BciT130I CCWGG 6 cut(s) 401, 480, 639, 796, 927, 1124
BcoDI GTCTC 1 cut(s) 1290
BfaI CTAG 3 cut(s) 72, 563, 1512
BfmI CTRYAG 3 cut(s) 258, 1300, 1675
BfoI RGCGCY 1 cut(s) 2018
BfrI CTTAAG 1 cut(s) 1024
BfuAI ACCTGC 1 cut(s) 1981
BglI GCCNNNNNGGC 1 cut(s) 1311
BisI GCNGC 2 cut(s) 883, 886
BlsI GCNGC 2 cut(s) 884, 887
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 6 cut(s) 401, 480, 639, 796, 927, 1124
Bme18I GGWCC 2 cut(s) 1200, 1421
BmeT110I CYCGRG 1 cut(s) 1260
BmgT120I GGNCC 2 cut(s) 1200, 1421
BmiI GGNNCC 4 cut(s) 398, 1641, 1938, 2047
BmrFI CCNGG 6 cut(s) 401, 480, 639, 796, 927, 1124
BmrI ACTGGG 4 cut(s) 161, 330, 552, 934
BmsI GCATC 5 cut(s) 85, 474, 654, 971, 1890
BmuI ACTGGG 4 cut(s) 161, 330, 552, 934
BoxI GACNNNNGTC 1 cut(s) 994
BpmI CTGGAG 2 cut(s) 291, 795
Bpu14I TTCGAA 2 cut(s) 558, 1162
BpuEI CTTGAG 1 cut(s) 1764
Bsa29I ATCGAT 1 cut(s) 105
BsaJI CCNNGG 4 cut(s) 479, 1105, 1203, 1417
Bsc4I CCNNNNNNNGG 3 cut(s) 1546, 2055, 2056
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 2 cut(s) 1396, 1994
BseBI CCWGG 6 cut(s) 401, 480, 639, 796, 927, 1124
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 4 cut(s) 479, 1105, 1203, 1417
BseGI GGATG 4 cut(s) 489, 986, 1174, 1604
BseLI CCNNNNNNNGG 3 cut(s) 1546, 2055, 2056
BseMI GCAATG 2 cut(s) 1396, 1994
BseMII CTCAG 5 cut(s) 663, 669, 740, 1328, 1932
BseRI GAGGAG 2 cut(s) 1241, 1949
BseXI GCAGC 2 cut(s) 872, 894
BsgI GTGCAG 1 cut(s) 1645
Bsh1236I CGCG 2 cut(s) 303, 1385
BshFI GGCC 2 cut(s) 1154, 1687
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 783, 1343
BsiHKCI CYCGRG 1 cut(s) 1260
BsiSI CCGG 1 cut(s) 2012
BslFI GGGAC 1 cut(s) 1961
BslI CCNNNNNNNGG 3 cut(s) 1546, 2055, 2056
BsmAI GTCTC 1 cut(s) 1290
BsmFI GGGAC 1 cut(s) 1961
BsmI GAATGC 1 cut(s) 1094
BsnI GGCC 2 cut(s) 1154, 1687
BsoBI CYCGRG 1 cut(s) 1260
Bsp119I TTCGAA 2 cut(s) 558, 1162
Bsp1286I GDGCHC 2 cut(s) 783, 1343
Bsp1407I TGTACA 1 cut(s) 1371
Bsp143I GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
BspACI CCGC 3 cut(s) 301, 431, 905
BspANI GGCC 2 cut(s) 1154, 1687
BspCNI CTCAG 5 cut(s) 664, 668, 739, 1329, 1933
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 2 cut(s) 303, 1385
BspLI GGNNCC 4 cut(s) 398, 1641, 1938, 2047
BspMAI CTGCAG 2 cut(s) 262, 1679
BspMI ACCTGC 1 cut(s) 1981
BspPI GGATC 2 cut(s) 121, 1215
BspT104I TTCGAA 2 cut(s) 558, 1162
BspTI CTTAAG 1 cut(s) 1024
BsrDI GCAATG 2 cut(s) 1396, 1994
BsrGI TGTACA 1 cut(s) 1371
BssECI CCNNGG 4 cut(s) 479, 1105, 1203, 1417
BssMI GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
BssT1I CCWWGG 2 cut(s) 1105, 1417
Bst2UI CCWGG 6 cut(s) 401, 480, 639, 796, 927, 1124
Bst4CI ACNGT 6 cut(s) 223, 376, 993, 1159, 1301, 1708
Bst6I CTCTTC 1 cut(s) 258
BstAFI CTTAAG 1 cut(s) 1024
BstAUI TGTACA 1 cut(s) 1371
BstBI TTCGAA 2 cut(s) 558, 1162
BstC8I GCNNGC 3 cut(s) 1102, 1387, 1879
BstDEI CTNAG 6 cut(s) 655, 672, 726, 1337, 1669, 1941
BstDSI CCRYGG 1 cut(s) 1203
BstF5I GGATG 4 cut(s) 489, 986, 1174, 1604
BstFNI CGCG 2 cut(s) 303, 1385
BstH2I RGCGCY 1 cut(s) 2018
BstHHI GCGC 3 cut(s) 47, 838, 2017
BstKTI GATC 6 cut(s) 116, 568, 847, 1174, 1210, 1219
BstMAI GTCTC 1 cut(s) 1290
BstMBI GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 242, 1311, 1391
BstNI CCWGG 6 cut(s) 401, 480, 639, 796, 927, 1124
BstNSI RCATGY 1 cut(s) 1881
BstPAI GACNNNNGTC 1 cut(s) 994
BstSCI CCNGG 6 cut(s) 399, 478, 637, 794, 925, 1122
BstSFI CTRYAG 3 cut(s) 258, 1300, 1675
BstUI CGCG 2 cut(s) 303, 1385
BstV1I GCAGC 2 cut(s) 872, 894
BstX2I RGATCY 1 cut(s) 1207
BstYI RGATCY 1 cut(s) 1207
Bsu15I ATCGAT 1 cut(s) 105
Bsu36I CCTNAGG 1 cut(s) 672
BsuRI GGCC 2 cut(s) 1154, 1687
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 1203
BtsCI GGATG 4 cut(s) 489, 986, 1174, 1604
BtsI GCAGTG 3 cut(s) 821, 859, 1182
BtsIMutI CAGTG 7 cut(s) 267, 381, 821, 859, 1084, 1155, 1182
BveI ACCTGC 1 cut(s) 1981
Cac8I GCNNGC 3 cut(s) 1102, 1387, 1879
CfoI GCGC 3 cut(s) 47, 838, 2017
Cfr13I GGNCC 2 cut(s) 1200, 1421
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 1 cut(s) 656
CsiI ACCWGGT 1 cut(s) 399
Csp6I GTAC 4 cut(s) 32, 1372, 1464, 1776
CviAII CATG 5 cut(s) 516, 1009, 1369, 1878, 2042
CviQI GTAC 4 cut(s) 32, 1372, 1464, 1776
DdeI CTNAG 6 cut(s) 655, 672, 726, 1337, 1669, 1941
DpnI GATC 6 cut(s) 115, 567, 846, 1173, 1209, 1218
DpnII GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
EaeI YGGCCR 1 cut(s) 1685
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 1105, 1417
Eco47I GGWCC 2 cut(s) 1200, 1421
Eco57I CTGAAG 1 cut(s) 1602
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 1260
EcoRI GAATTC 2 cut(s) 706, 766
EcoRII CCWGG 6 cut(s) 399, 478, 637, 794, 925, 1122
EcoT14I CCWWGG 2 cut(s) 1105, 1417
EcoT22I ATGCAT 1 cut(s) 1883
ErhI CCWWGG 2 cut(s) 1105, 1417
FaeI CATG 5 cut(s) 519, 1012, 1372, 1881, 2045
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 1 cut(s) 1961
FatI CATG 5 cut(s) 515, 1008, 1368, 1877, 2041
FauI CCCGC 1 cut(s) 912
FauNDI CATATG 2 cut(s) 194, 1001
Fnu4HI GCNGC 2 cut(s) 883, 886
FokI GGATG 4 cut(s) 496, 993, 1181, 1591
Fsp4HI GCNGC 2 cut(s) 883, 886
FspBI CTAG 3 cut(s) 72, 563, 1512
GlaI GCGC 3 cut(s) 46, 837, 2016
GluI GCNGC 2 cut(s) 883, 886
GsuI CTGGAG 2 cut(s) 291, 795
HaeII RGCGCY 1 cut(s) 2018
HaeIII GGCC 2 cut(s) 1154, 1687
HapII CCGG 1 cut(s) 2012
HgaI GACGC 1 cut(s) 656
HhaI GCGC 3 cut(s) 47, 838, 2017
Hin1II CATG 5 cut(s) 519, 1012, 1372, 1881, 2045
Hin6I GCGC 3 cut(s) 45, 836, 2015
HinP1I GCGC 3 cut(s) 45, 836, 2015
HincII GTYRAC 3 cut(s) 339, 426, 510
HindII GTYRAC 3 cut(s) 339, 426, 510
HpaII CCGG 1 cut(s) 2012
HphI GGTGA 7 cut(s) 152, 351, 431, 445, 772, 1048, 1247
Hpy166II GTNNAC 7 cut(s) 339, 426, 510, 594, 1039, 1203, 1466
Hpy188III TCNNGA 5 cut(s) 569, 671, 1512, 1781, 1984
Hpy8I GTNNAC 7 cut(s) 339, 426, 510, 594, 1039, 1203, 1466
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 8 cut(s) 284, 565, 566, 1030, 1158, 1546, 1626, 1778
HpyCH4III ACNGT 6 cut(s) 223, 376, 993, 1159, 1301, 1708
HpyCH4IV ACGT 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 1311, 1391
HpyF3I CTNAG 6 cut(s) 655, 672, 726, 1337, 1669, 1941
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 5 cut(s) 519, 1012, 1372, 1881, 2045
HspAI GCGC 3 cut(s) 45, 836, 2015
Kzo9I GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
LmnI GCTCC 3 cut(s) 519, 1639, 1936
Lsp1109I GCAGC 2 cut(s) 872, 894
LweI GCATC 5 cut(s) 85, 474, 654, 971, 1890
MabI ACCWGGT 1 cut(s) 399
MaeI CTAG 3 cut(s) 72, 563, 1512
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 6 cut(s) 208, 316, 497, 617, 1072, 1235
MalI GATC 6 cut(s) 115, 567, 846, 1173, 1209, 1218
MboI GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 1207
MhlI GDGCHC 2 cut(s) 783, 1343
MlsI TGGCCA 1 cut(s) 1687
MluNI TGGCCA 1 cut(s) 1687
MlyI GAGTC 3 cut(s) 1067, 1238, 1935
MmeI TCCRAC 1 cut(s) 485
Mox20I TGGCCA 1 cut(s) 1687
Mph1103I ATGCAT 1 cut(s) 1883
MroXI GAANNNNTTC 2 cut(s) 252, 355
MscI TGGCCA 1 cut(s) 1687
MslI CAYNNNNRTG 2 cut(s) 1034, 1999
Msp20I TGGCCA 1 cut(s) 1687
MspA1I CMGCKG 3 cut(s) 431, 885, 907
MspCI CTTAAG 1 cut(s) 1024
MspI CCGG 1 cut(s) 2012
MspR9I CCNGG 6 cut(s) 401, 480, 639, 796, 927, 1124
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 1094
MvaI CCWGG 6 cut(s) 401, 480, 639, 796, 927, 1124
MvnI CGCG 2 cut(s) 303, 1385
MwoI GCNNNNNNNGC 3 cut(s) 242, 1311, 1391
NdeI CATATG 2 cut(s) 194, 1001
NdeII GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
NlaIII CATG 5 cut(s) 519, 1012, 1372, 1881, 2045
NlaIV GGNNCC 4 cut(s) 398, 1641, 1938, 2047
NmuCI GTSAC 2 cut(s) 208, 1235
NsiI ATGCAT 1 cut(s) 1883
NspI RCATGY 1 cut(s) 1881
NspV TTCGAA 2 cut(s) 558, 1162
PaeI GCATGC 1 cut(s) 1881
PaeR7I CTCGAG 1 cut(s) 1260
PctI GAATGC 1 cut(s) 1094
PdmI GAANNNNTTC 2 cut(s) 252, 355
PkrI GCNGC 2 cut(s) 884, 887
PleI GAGTC 3 cut(s) 1066, 1237, 1934
PpsI GAGTC 3 cut(s) 1066, 1237, 1934
PshAI GACNNNNGTC 1 cut(s) 994
PshBI ATTAAT 3 cut(s) 861, 894, 1401
PsiI TTATAA 1 cut(s) 1448
Psp6I CCWGG 6 cut(s) 399, 478, 637, 794, 925, 1122
PspGI CCWGG 6 cut(s) 399, 478, 637, 794, 925, 1122
PspN4I GGNNCC 4 cut(s) 398, 1641, 1938, 2047
PspPI GGNCC 2 cut(s) 1200, 1421
PspXI VCTCGAGB 1 cut(s) 1260
PstI CTGCAG 2 cut(s) 262, 1679
PsuI RGATCY 1 cut(s) 1207
PvuII CAGCTG 1 cut(s) 885
RsaI GTAC 4 cut(s) 33, 1373, 1465, 1777
RsaNI GTAC 4 cut(s) 32, 1372, 1464, 1776
RseI CAYNNNNRTG 2 cut(s) 1034, 1999
SatI GCNGC 2 cut(s) 883, 886
Sau3AI GATC 6 cut(s) 113, 565, 844, 1171, 1207, 1216
Sau96I GGNCC 2 cut(s) 1200, 1421
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 3 cut(s) 1067, 1238, 1935
ScrFI CCNGG 6 cut(s) 401, 480, 639, 796, 927, 1124
SduI GDGCHC 2 cut(s) 783, 1343
SexAI ACCWGGT 1 cut(s) 399
SfaNI GCATC 5 cut(s) 85, 474, 654, 971, 1890
SfcI CTRYAG 3 cut(s) 258, 1300, 1675
Sfr274I CTCGAG 1 cut(s) 1260
SfuI TTCGAA 2 cut(s) 558, 1162
SinI GGWCC 2 cut(s) 1200, 1421
SlaI CTCGAG 1 cut(s) 1260
SmiMI CAYNNNNRTG 2 cut(s) 1034, 1999
SmlI CTYRAG 3 cut(s) 1024, 1260, 1779
SmoI CTYRAG 3 cut(s) 1024, 1260, 1779
SphI GCATGC 1 cut(s) 1881
SsiI CCGC 3 cut(s) 301, 431, 905
SspI AATATT 1 cut(s) 647
SspMI CTAG 3 cut(s) 72, 563, 1512
StyD4I CCNGG 6 cut(s) 399, 478, 637, 794, 925, 1122
StyI CCWWGG 2 cut(s) 1105, 1417
TaaI ACNGT 6 cut(s) 223, 376, 993, 1159, 1301, 1708
TaiI ACGT 1 cut(s) 15
TaqI TCGA 6 cut(s) 105, 214, 558, 1064, 1162, 1261
TaqII GACCGA 1 cut(s) 549
TatI WGTACW 4 cut(s) 31, 1371, 1463, 1775
TscAI CASTG 7 cut(s) 274, 381, 821, 859, 1084, 1162, 1189
TseFI GTSAC 2 cut(s) 208, 1235
TseI GCWGC 2 cut(s) 882, 885
Tsp45I GTSAC 2 cut(s) 208, 1235
TspDTI ATGAA 9 cut(s) 463, 504, 779, 1025, 1131, 1419, 1465, 1511, 1593
TspGWI ACGGA 1 cut(s) 1220
TspRI CASTG 7 cut(s) 274, 381, 821, 859, 1084, 1162, 1189
Vha464I CTTAAG 1 cut(s) 1024
VpaK11BI GGWCC 2 cut(s) 1200, 1421
VspI ATTAAT 3 cut(s) 861, 894, 1401
XapI RAATTY 6 cut(s) 694, 706, 766, 1406, 1583, 1744
XbaI TCTAGA 1 cut(s) 1511
XceI RCATGY 1 cut(s) 1881
XhoI CTCGAG 1 cut(s) 1260
XmnI GAANNNNTTC 2 cut(s) 252, 355
XspI CTAG 3 cut(s) 72, 563, 1512
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 1883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.