pycom09g02410

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000412
Physical Location & Seq
Forward (+)
1816082 .. 1820097
4016 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02410.6

Sequence Viewer

Length: 3054 bp
ATGCTGTTTTCCATCTTCGTTTCGTATGCTGTAGCAGTCTTGTTGTGCTCATCTCACCCATTTTGCCGAGCTAGAGATACAATAACATACGGCACTCCAATGAATGAATCAGAAACTCTTGTTTCATCTGGAGGAAGATTTGAACTCGGATTCTTTAGCCCTTCACCTAAAGGGACTCAAAGATATGTTGGCATATGGTATCACCAACTGAGTCCGAGGACAGTGGTATGGGTTGCCAACAGAGAAAAGCCAGTTCTCAATTCCACTGGTACCAGACTCCTTGCACTCCGAGATGATGACCTCCAAGTGTTGGATGCTACTGGAAAGAGTTACTGGTCAACGGGGCTTGAAACATCCAAGTCTTCAGCACGGACTGCGAAGCTCATGGATTCCGGGAACCTTGTTCTAAGCAGTGGGGATGATCAGTTGGCAGAGAAATTTTTATGGCAGAGCTTTCAAAGTCCAACTGATACATTCATTCCTGGGATGATGATGGGTGGAAGCTTGGTGTTGACTTCGTGGACAGGCAAAGAAGACCCGGCAAGCGGGCAATTCAGCTTCAAACAAGATCCAGATAAAGAGGACCAGTATGTCATCACAAATTTAGAGTCGATCACGTACTGGAAAGGCGGAGAACGGGCATCTGATACAATTGACAGCTTTGATCAAATGCTCCTTGAAGTTAGTGACTTATTATCAAATTTCAGGAAGAATGTTCGAAATTCCAACAGCATTCTGAATAAAAATAAATATGCAAGGTTGGTGCTTAACATTACTGGAGAGATACAGTTTTGGAAGTGGATTAATTCCACAAAACAGTGGCATTTGTATTGGTGGGCGCCAAGAGATAAATGCAGTGTGTTTAATGCTTGCGGCAACTTTGGCAGTTGCAATGTTAATAATATGCCGTTTCTGTGCAAATGTTTGCCAGGTTTCAAACCCCTGTATCCACAGCAGTGGAATTCTGGAGACTTTTCAGGTGGATGCACAAGAAAGTCCGCATTATGTGACAACAACAAGAATTATACCTTCTTGAGCTTGAAGAAGATGAAAGTGGGAAACCCAGATTCACATATCAACGTTAGTGAAACAGAATGCAGGAACCAGTGCTTAAATGAGTGCCAGTGCAAGGCTTATTCATATGCAAAATCTGAAAACTATAGTCCTCGAAGGCAGGCGCATGGGCCTACTTCATCGTGCAGAATATGGAAGGTGGGAGATCTAAATAATCCTGAAGAAGAGTATACTGATGGGGGCCAGGACCTCTCTGTTCGTGTTGCATTATCTGATTTAGAATCAACTGTAAGAGACTGTAAGCCTTGTGGCACAACCATCATTCCTTATCCCCTGAGCACAAGACTGGGTTGCGGTGACCCCATGTACTTCCGTTTCAAGTGCAACGACTTCACAGGTCAGGTTAACTTTGTGGGACTGAATGAGACCTTGTTTCCAGTTATTAGCATAAACCCAAGTACACAGAGATTTTTCATCCAAGGCTTCCAGACCGAAAATGTAGATAACTGTGATCCTAGAAACAGAGGGGCTGATCTGCAGATCAATCCATCGTTTCCATTTGAAGTAATCAGTCCGTGTAATGCTGACCTGGGTAATTTTAGTTCTGAAGTACTATCATCCGGAGGTCCTAATGTAGTGGGGATGCGTTGGGAGCTACCAATGGAACCAACCTGTACAACATCTGCAGACTGCAGGGATTGGGCACATTCAATTTGCAAGCGTGCACAAGATGGAAAGAAAAGATGCCTGTGCGAAACCAATTTTCGATGGAATGGCTTGATATTAAGATGTACTCAAGAAGGTAATCTTCTAGGGCCACCAAAAGAAGAGCATACGAGAAGAAAATTGACATTGTCTCTTATAATTGTAGCGGTGCTTTTAAGTGTGATTTTTCTGGCGTGCATCGTCATTTCCATTTATATATGGCGAAGAAATATTAACAACAAACGAGATCAAATAAGACGAGGACAGTTTGATAGTGAAAGGAGAGTCAAGGAGTTGATAGACACAAGCGAGTTCAAGGAAGAGGAAGGTATAGATGTACCTTTTTTTGATATGCAAACTATACTAGACGCTACAGATAATTTCTCAAATGCAAACAAGCTTGGACAAGGGGGATACGGGCCTGTTTACAAGGGAATGTTTATTGGAGGCCAAGAAATCGCGGTGAAAAGGCTATCAAGGGTTTCAGGACAAGGCTTGCAGGAATTTAGGAATGAGGTGGTGCTGATTGCCAAACTTCAACACCGAAACCTTGTTAGACTTAAGGGATATTGCATGAAAGGAGAAGAAAAGATCTTACTCTATGAGTACATGCCTAACAAAAGTTTAGACTTCTTTATATTCGATCATACAAAAAGCATGTTTCTCAACTGGGAGATGCGTTTTAGCATCATTTTGGGAATCGCTCGAGGGCTTCTTTATCTTCATCAAGATTCCAGATTGAGGATCATTCATAGAGATTTGAAAACCAGCAACATTCTCCTAGATGAGGAGATGAACCCCAAAATATCTGACTTCGGTTTGGCGAGGATTGTTGGAGGCAAGGAAACTCAGGCAAACACAAACACTGTTGTTGGAACTTATGGTTACATGGCTCCGGAGTATGCATTGGACGGAACTTTCTCAGTGAAATCAGATGTCTTTAGCTTTGGCGTGGTTCTTCTTGAGATAATCAGCGGAAAAAAGAATACAGGCTTTTACCAATCCGAACAAACTTTCAGCCTCATAAATTATGCATGGAGCCTCTGGACAGAAAACAAAGTGCTGGATTTAATGGACAAGACTTTGGACGAAAGTTGCAACAAAAACCAGTTTATCCAGTGTGTCAACGTCGGCCTCTTATGCGTACAAGAAGATCCAAACGATCGGCCCGCCATGTCAAACGTTATCACCCTGCTTGACAGTGATACTGCAACCTCTACATCTCCTAAACAACCAGGGTTTCTCACAAGGAGAGGCAAATCCAGCACAGCTTCTTCTTCTAGTAAGCCAGAAACTATATCTGAAATAACCACCAGTACAGTAGAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1018

Amino Acids

114.61

Weight (kDa)

7.35

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1877
AasI GACNNNNNNGTC 1 cut(s) 590
Acc65I GGTACC 1 cut(s) 269
AccB1I GGYRCC 2 cut(s) 269, 838
AccB7I CCANNNNNTGG 1 cut(s) 310
AccI GTMKAC 1 cut(s) 1244
AccII CGCG 1 cut(s) 2180
AccIII TCCGGA 2 cut(s) 1634, 2614
AciI CCGC 9 cut(s) 546, 630, 873, 999, 1368, 1886, 2180, 2694, 2889
AclI AACGTT 2 cut(s) 1080, 2901
AclWI GGATC 4 cut(s) 563, 1520, 2471, 2867
AcsI RAATTY 6 cut(s) 437, 601, 700, 721, 961, 2222
AcuI CTGAAG 3 cut(s) 348, 1254, 1641
AcyI GRCGYC 1 cut(s) 839
AfiI CCNNNNNNNGG 5 cut(s) 310, 545, 1129, 2460, 2506
AflII CTTAAG 1 cut(s) 2279
AjnI CCWGG 5 cut(s) 481, 928, 1257, 1602, 2953
AleI CACNNNNGTG 1 cut(s) 955
Alw21I GWGCWC 3 cut(s) 50, 1355, 1741
Alw26I GTCTC 4 cut(s) 963, 1302, 1433, 1875
Alw44I GTGCAC 1 cut(s) 1737
AlwI GGATC 4 cut(s) 563, 1520, 2471, 2867
Ama87I CYCGRG 1 cut(s) 2424
Aor13HI TCCGGA 2 cut(s) 1634, 2614
AoxI GGCC 7 cut(s) 1184, 1255, 1829, 2138, 2167, 2851, 2885
ApaLI GTGCAC 1 cut(s) 1737
ApoI RAATTY 6 cut(s) 437, 601, 700, 721, 961, 2222
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
AseI ATTAAT 1 cut(s) 804
Asp718I GGTACC 1 cut(s) 269
AspLEI GCGC 2 cut(s) 841, 1180
AspS9I GGNCC 8 cut(s) 583, 1184, 1255, 1261, 1640, 1829, 2138, 2886
AsuC2I CCSGG 2 cut(s) 394, 539
AsuHPI GGTGA 6 cut(s) 47, 156, 194, 1382, 2194, 2899
AsuII TTCGAA 1 cut(s) 718
AvaI CYCGRG 1 cut(s) 2424
AvaII GGWCC 3 cut(s) 583, 1261, 1640
BaeGI GKGCMC 2 cut(s) 1720, 1741
BaeI ACNNNNGTAYC 2 cut(s) 929, 962
BanI GGYRCC 2 cut(s) 269, 838
BbsI GAAGAC 2 cut(s) 354, 540
Bbv12I GWGCWC 3 cut(s) 50, 1355, 1741
BccI CCATC 7 cut(s) 20, 487, 1244, 1340, 1570, 1739, 1776
BceAI ACGGC 2 cut(s) 106, 892
BciT130I CCWGG 5 cut(s) 483, 930, 1259, 1604, 2955
BciVI GTATCC 2 cut(s) 957, 2126
BclI TGATCA 2 cut(s) 421, 664
BcnI CCSGG 2 cut(s) 394, 539
BcoDI GTCTC 4 cut(s) 963, 1302, 1433, 1875
BfaI CTAG 6 cut(s) 72, 1530, 1826, 2084, 2501, 3000
BfmI CTRYAG 6 cut(s) 30, 1159, 1550, 1698, 1705, 2091
BfoI RGCGCY 1 cut(s) 842
BfrI CTTAAG 1 cut(s) 2279
BfuI GTATCC 2 cut(s) 957, 2126
BglII AGATCT 2 cut(s) 1219, 2310
BisI GCNGC 1 cut(s) 874
BlsI GCNGC 1 cut(s) 875
BmcAI AGTACT 1 cut(s) 1626
Bme1390I CCNGG 7 cut(s) 394, 483, 539, 930, 1259, 1604, 2955
Bme18I GGWCC 3 cut(s) 583, 1261, 1640
BmeT110I CYCGRG 1 cut(s) 2424
BmgT120I GGNCC 8 cut(s) 583, 1184, 1255, 1261, 1640, 1829, 2138, 2886
BmiI GGNNCC 8 cut(s) 271, 398, 840, 1103, 1256, 1680, 2613, 2759
BmrFI CCNGG 7 cut(s) 394, 483, 539, 930, 1259, 1604, 2955
BmrI ACTGGG 2 cut(s) 1370, 2398
BmsI GCATC 8 cut(s) 304, 650, 974, 1647, 1748, 1926, 2385, 2415
BmuI ACTGGG 2 cut(s) 1370, 2398
BpiI GAAGAC 2 cut(s) 354, 540
BpmI CTGGAG 3 cut(s) 150, 798, 987
Bpu10I CCTNAGC 1 cut(s) 1349
Bpu14I TTCGAA 1 cut(s) 718
BpuEI CTTGAG 3 cut(s) 1054, 1794, 2702
BpuMI CCSGG 2 cut(s) 394, 539
BsaAI YACGTR 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 1433
BsaJI CCNNGG 5 cut(s) 215, 482, 1492, 1603, 2954
BsaWI WCCGGW 2 cut(s) 1634, 2614
Bsc4I CCNNNNNNNGG 5 cut(s) 310, 545, 1129, 2460, 2506
Bse3DI GCAATG 1 cut(s) 898
BseAI TCCGGA 2 cut(s) 1634, 2614
BseBI CCWGG 5 cut(s) 483, 930, 1259, 1604, 2955
BseDI CCNNGG 5 cut(s) 215, 482, 1492, 1603, 2954
BseGI GGATG 8 cut(s) 319, 353, 424, 492, 989, 1488, 1631, 1662
BseLI CCNNNNNNNGG 5 cut(s) 310, 545, 1129, 2460, 2506
BseMI GCAATG 1 cut(s) 898
BseMII CTCAG 4 cut(s) 200, 1340, 2582, 2655
BseRI GAGGAG 1 cut(s) 2522
BseSI GKGCMC 2 cut(s) 1720, 1741
BsgI GTGCAG 1 cut(s) 1219
Bsh1236I CGCG 1 cut(s) 2180
Bsh1285I CGRYCG 1 cut(s) 2884
BshFI GGCC 7 cut(s) 1186, 1257, 1831, 2140, 2169, 2853, 2887
BshNI GGYRCC 2 cut(s) 269, 838
BsiEI CGRYCG 1 cut(s) 2884
BsiHKAI GWGCWC 3 cut(s) 50, 1355, 1741
BsiHKCI CYCGRG 1 cut(s) 2424
BsiSI CCGG 4 cut(s) 393, 539, 1635, 2615
BslFI GGGAC 2 cut(s) 187, 1443
BslI CCNNNNNNNGG 5 cut(s) 310, 545, 1129, 2460, 2506
BsmAI GTCTC 4 cut(s) 963, 1302, 1433, 1875
BsmFI GGGAC 2 cut(s) 187, 1443
BsmI GAATGC 2 cut(s) 732, 1100
BsnI GGCC 7 cut(s) 1186, 1257, 1831, 2140, 2169, 2853, 2887
Bso31I GGTCTC 1 cut(s) 1433
BsoBI CYCGRG 1 cut(s) 2424
Bsp119I TTCGAA 1 cut(s) 718
Bsp1286I GDGCHC 4 cut(s) 50, 1355, 1720, 1741
Bsp13I TCCGGA 2 cut(s) 1634, 2614
Bsp1407I TGTACA 1 cut(s) 1688
BspACI CCGC 9 cut(s) 546, 630, 873, 999, 1368, 1886, 2180, 2694, 2889
BspANI GGCC 7 cut(s) 1186, 1257, 1831, 2140, 2169, 2853, 2887
BspCNI CTCAG 4 cut(s) 201, 1341, 2581, 2654
BspEI TCCGGA 2 cut(s) 1634, 2614
BspFNI CGCG 1 cut(s) 2180
BspLI GGNNCC 8 cut(s) 271, 398, 840, 1103, 1256, 1680, 2613, 2759
BspMAI CTGCAG 3 cut(s) 1554, 1702, 1709
BspPI GGATC 4 cut(s) 563, 1520, 2471, 2867
BspQI GCTCTTC 1 cut(s) 1836
BspT104I TTCGAA 1 cut(s) 718
BspT107I GGYRCC 2 cut(s) 269, 838
BspTI CTTAAG 1 cut(s) 2279
BspTNI GGTCTC 1 cut(s) 1433
BsrDI GCAATG 1 cut(s) 898
BsrGI TGTACA 1 cut(s) 1688
BssECI CCNNGG 5 cut(s) 215, 482, 1492, 1603, 2954
BssNAI GTATAC 1 cut(s) 1245
BssNI GRCGYC 1 cut(s) 839
BssT1I CCWWGG 1 cut(s) 1492
Bst1107I GTATAC 1 cut(s) 1245
Bst2UI CCWGG 5 cut(s) 483, 930, 1259, 1604, 2955
Bst6I CTCTTC 3 cut(s) 1233, 1836, 2034
BstACI GRCGYC 1 cut(s) 839
BstAFI CTTAAG 1 cut(s) 2279
BstAPI GCANNNNNTGC 1 cut(s) 374
BstAUI TGTACA 1 cut(s) 1688
BstBAI YACGTR 1 cut(s) 618
BstBI TTCGAA 1 cut(s) 718
BstC8I GCNNGC 9 cut(s) 544, 548, 871, 1176, 1733, 1737, 1915, 2216, 2889
BstDEI CTNAG 5 cut(s) 209, 407, 1349, 2568, 2641
BstEII GGTNACC 1 cut(s) 1370
BstENI CCTNNNNNAGG 1 cut(s) 2504
BstF5I GGATG 8 cut(s) 319, 353, 424, 492, 989, 1488, 1631, 1662
BstFNI CGCG 1 cut(s) 2180
BstH2I RGCGCY 1 cut(s) 842
BstHHI GCGC 2 cut(s) 841, 1180
BstMAI GTCTC 4 cut(s) 963, 1302, 1433, 1875
BstMCI CGRYCG 1 cut(s) 2884
BstMWI GCNNNNNNNGC 5 cut(s) 374, 882, 1666, 2859, 2982
BstNI CCWGG 5 cut(s) 483, 930, 1259, 1604, 2955
BstNSI RCATGY 2 cut(s) 2332, 2380
BstPI GGTNACC 1 cut(s) 1370
BstSCI CCNGG 7 cut(s) 392, 481, 537, 928, 1257, 1602, 2953
BstSFI CTRYAG 6 cut(s) 30, 1159, 1550, 1698, 1705, 2091
BstSLI GKGCMC 2 cut(s) 1720, 1741
BstUI CGCG 1 cut(s) 2180
BstV2I GAAGAC 2 cut(s) 354, 540
BstX2I RGATCY 4 cut(s) 568, 1219, 2310, 2872
BstXI CCANNNNNNTGG 1 cut(s) 957
BstYI RGATCY 4 cut(s) 568, 1219, 2310, 2872
BstZ17I GTATAC 1 cut(s) 1245
BsuI GTATCC 2 cut(s) 957, 2126
BsuRI GGCC 7 cut(s) 1186, 1257, 1831, 2140, 2169, 2853, 2887
BtsCI GGATG 8 cut(s) 319, 353, 424, 492, 989, 1488, 1631, 1662
BtsI GCAGTG 3 cut(s) 418, 862, 962
Cac8I GCNNGC 9 cut(s) 544, 548, 871, 1176, 1733, 1737, 1915, 2216, 2889
CfoI GCGC 2 cut(s) 841, 1180
Cfr13I GGNCC 8 cut(s) 583, 1184, 1255, 1261, 1640, 1829, 2138, 2886
CseI GACGC 1 cut(s) 2096
CviAII CATG 9 cut(s) 385, 1181, 1378, 2293, 2329, 2377, 2608, 2754, 2893
DdeI CTNAG 5 cut(s) 209, 407, 1349, 2568, 2641
DinI GGCGCC 1 cut(s) 840
DrdI GACNNNNNNGTC 1 cut(s) 590
DseDI GACNNNNNNGTC 1 cut(s) 590
Eam1104I CTCTTC 3 cut(s) 1233, 1836, 2034
EarI CTCTTC 3 cut(s) 1233, 1836, 2034
EciI GGCGGA 1 cut(s) 645
Eco130I CCWWGG 1 cut(s) 1492
Eco31I GGTCTC 1 cut(s) 1433
Eco47I GGWCC 3 cut(s) 583, 1261, 1640
Eco57I CTGAAG 3 cut(s) 348, 1254, 1641
Eco88I CYCGRG 1 cut(s) 2424
Eco91I GGTNACC 1 cut(s) 1370
EcoNI CCTNNNNNAGG 1 cut(s) 2504
EcoO109I RGGNCCY 2 cut(s) 1261, 1640
EcoO65I GGTNACC 1 cut(s) 1370
EcoRI GAATTC 1 cut(s) 961
EcoRII CCWGG 5 cut(s) 481, 928, 1257, 1602, 2953
EcoT14I CCWWGG 1 cut(s) 1492
EcoT22I ATGCAT 2 cut(s) 2626, 2755
EgeI GGCGCC 1 cut(s) 840
EheI GGCGCC 1 cut(s) 840
ErhI CCWWGG 1 cut(s) 1492
FaeI CATG 9 cut(s) 388, 1184, 1381, 2296, 2332, 2380, 2611, 2757, 2896
FalI AAGNNNNNCTT 2 cut(s) 1806, 1838
FaqI GGGAC 2 cut(s) 187, 1443
FatI CATG 9 cut(s) 384, 1180, 1377, 2292, 2328, 2376, 2607, 2753, 2892
FauI CCCGC 2 cut(s) 539, 2896
FauNDI CATATG 2 cut(s) 194, 1141
FbaI TGATCA 2 cut(s) 421, 664
FblI GTMKAC 1 cut(s) 1244
Fnu4HI GCNGC 1 cut(s) 874
FokI GGATG 8 cut(s) 326, 340, 431, 499, 996, 1475, 1618, 1669
Fsp4HI GCNGC 1 cut(s) 874
FspBI CTAG 6 cut(s) 72, 1530, 1826, 2084, 2501, 3000
GlaI GCGC 2 cut(s) 840, 1179
GluI GCNGC 1 cut(s) 874
GsuI CTGGAG 3 cut(s) 150, 798, 987
HaeII RGCGCY 1 cut(s) 842
HaeIII GGCC 7 cut(s) 1186, 1257, 1831, 2140, 2169, 2853, 2887
HapII CCGG 4 cut(s) 393, 539, 1635, 2615
HgaI GACGC 1 cut(s) 2096
HhaI GCGC 2 cut(s) 841, 1180
Hin1I GRCGYC 1 cut(s) 839
Hin1II CATG 9 cut(s) 388, 1184, 1381, 2296, 2332, 2380, 2611, 2757, 2896
Hin6I GCGC 2 cut(s) 839, 1178
HinP1I GCGC 2 cut(s) 839, 1178
HincII GTYRAC 4 cut(s) 339, 513, 1420, 2845
HindII GTYRAC 4 cut(s) 339, 513, 1420, 2845
HindIII AAGCTT 2 cut(s) 502, 2117
HpaI GTTAAC 1 cut(s) 1420
HpaII CCGG 4 cut(s) 393, 539, 1635, 2615
HphI GGTGA 6 cut(s) 47, 156, 194, 1382, 2194, 2899
Hpy166II GTNNAC 9 cut(s) 339, 513, 522, 1245, 1420, 1475, 1739, 2146, 2845
Hpy8I GTNNAC 9 cut(s) 339, 513, 522, 1245, 1420, 1475, 1739, 2146, 2845
Hpy99I CGWCG 1 cut(s) 2852
HpyAV CCTTC 7 cut(s) 171, 1039, 1164, 1204, 1808, 2039, 3038
HpyCH4IV ACGT 4 cut(s) 617, 1080, 2847, 2901
HpyF10VI GCNNNNNNNGC 5 cut(s) 374, 882, 1666, 2859, 2982
HpyF3I CTNAG 5 cut(s) 209, 407, 1349, 2568, 2641
HpySE526I ACGT 4 cut(s) 617, 1080, 2847, 2901
Hsp92I GRCGYC 1 cut(s) 839
Hsp92II CATG 9 cut(s) 388, 1184, 1381, 2296, 2332, 2380, 2611, 2757, 2896
HspAI GCGC 2 cut(s) 839, 1178
KasI GGCGCC 1 cut(s) 838
Kpn2I TCCGGA 2 cut(s) 1634, 2614
KpnI GGTACC 1 cut(s) 273
Ksp22I TGATCA 2 cut(s) 421, 664
KspAI GTTAAC 1 cut(s) 1420
LguI GCTCTTC 1 cut(s) 1836
LmnI GCTCC 4 cut(s) 678, 1666, 2617, 2757
LweI GCATC 8 cut(s) 304, 650, 974, 1647, 1748, 1926, 2385, 2415
MaeI CTAG 6 cut(s) 72, 1530, 1826, 2084, 2501, 3000
MaeII ACGT 4 cut(s) 617, 1080, 2847, 2901
MaeIII GTNAC 5 cut(s) 329, 686, 1007, 1370, 2603
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 4 cut(s) 568, 1219, 2310, 2872
MhlI GDGCHC 4 cut(s) 50, 1355, 1720, 1741
Mly113I GGCGCC 1 cut(s) 839
MlyI GAGTC 5 cut(s) 169, 220, 270, 617, 2013
MmeI TCCRAC 5 cut(s) 291, 488, 750, 2533, 2572
Mph1103I ATGCAT 2 cut(s) 2626, 2755
MroI TCCGGA 2 cut(s) 1634, 2614
MslI CAYNNNNRTG 2 cut(s) 98, 955
MspA1I CMGCKG 1 cut(s) 2694
MspCI CTTAAG 1 cut(s) 2279
MspI CCGG 4 cut(s) 393, 539, 1635, 2615
MspR9I CCNGG 7 cut(s) 394, 483, 539, 930, 1259, 1604, 2955
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 2 cut(s) 732, 1100
MvaI CCWGG 5 cut(s) 483, 930, 1259, 1604, 2955
MvnI CGCG 1 cut(s) 2180
MwoI GCNNNNNNNGC 5 cut(s) 374, 882, 1666, 2859, 2982
NarI GGCGCC 1 cut(s) 839
NciI CCSGG 2 cut(s) 394, 539
NdeI CATATG 2 cut(s) 194, 1141
NlaIII CATG 9 cut(s) 388, 1184, 1381, 2296, 2332, 2380, 2611, 2757, 2896
NlaIV GGNNCC 8 cut(s) 271, 398, 840, 1103, 1256, 1680, 2613, 2759
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 3 cut(s) 686, 1007, 1370
NsiI ATGCAT 2 cut(s) 2626, 2755
NspI RCATGY 2 cut(s) 2332, 2380
NspV TTCGAA 1 cut(s) 718
OliI CACNNNNGTG 1 cut(s) 955
PaeR7I CTCGAG 1 cut(s) 2424
PciSI GCTCTTC 1 cut(s) 1836
PctI GAATGC 2 cut(s) 732, 1100
PfeI GAWTC 7 cut(s) 107, 150, 389, 1067, 1295, 2418, 2450
PflFI GACNNNGTC 1 cut(s) 1867
PflMI CCANNNNNTGG 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 392
PkrI GCNGC 1 cut(s) 875
Ple19I CGATCG 1 cut(s) 2884
PleI GAGTC 5 cut(s) 169, 219, 270, 616, 2012
PluTI GGCGCC 1 cut(s) 842
PpsI GAGTC 5 cut(s) 169, 219, 270, 616, 2012
Ppu21I YACGTR 1 cut(s) 618
PpuMI RGGWCCY 2 cut(s) 1261, 1640
PshBI ATTAAT 1 cut(s) 804
PsiI TTATAA 1 cut(s) 1877
Psp1406I AACGTT 2 cut(s) 1080, 2901
Psp5II RGGWCCY 2 cut(s) 1261, 1640
Psp6I CCWGG 5 cut(s) 481, 928, 1257, 1602, 2953
PspEI GGTNACC 1 cut(s) 1370
PspGI CCWGG 5 cut(s) 481, 928, 1257, 1602, 2953
PspN4I GGNNCC 8 cut(s) 271, 398, 840, 1103, 1256, 1680, 2613, 2759
PspPI GGNCC 8 cut(s) 583, 1184, 1255, 1261, 1640, 1829, 2138, 2886
PspPPI RGGWCCY 2 cut(s) 1261, 1640
PspXI VCTCGAGB 1 cut(s) 2424
PsrI GAACNNNNNNTAC 2 cut(s) 1600, 1632
PstI CTGCAG 3 cut(s) 1554, 1702, 1709
PsuI RGATCY 4 cut(s) 568, 1219, 2310, 2872
PsyI GACNNNGTC 1 cut(s) 1867
PvuI CGATCG 1 cut(s) 2884
RseI CAYNNNNRTG 2 cut(s) 98, 955
SapI GCTCTTC 1 cut(s) 1836
SatI GCNGC 1 cut(s) 874
Sau96I GGNCC 8 cut(s) 583, 1184, 1255, 1261, 1640, 1829, 2138, 2886
ScaI AGTACT 1 cut(s) 1626
SchI GAGTC 5 cut(s) 169, 220, 270, 617, 2013
ScrFI CCNGG 7 cut(s) 394, 483, 539, 930, 1259, 1604, 2955
SduI GDGCHC 4 cut(s) 50, 1355, 1720, 1741
SfaNI GCATC 8 cut(s) 304, 650, 974, 1647, 1748, 1926, 2385, 2415
SfcI CTRYAG 6 cut(s) 30, 1159, 1550, 1698, 1705, 2091
SfoI GGCGCC 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 2424
SfuI TTCGAA 1 cut(s) 718
SinI GGWCC 3 cut(s) 583, 1261, 1640
SlaI CTCGAG 1 cut(s) 2424
SmiMI CAYNNNNRTG 2 cut(s) 98, 955
SmlI CTYRAG 5 cut(s) 1033, 1809, 2279, 2424, 2681
SmoI CTYRAG 5 cut(s) 1033, 1809, 2279, 2424, 2681
SsiI CCGC 9 cut(s) 546, 630, 873, 999, 1368, 1886, 2180, 2694, 2889
SspDI GGCGCC 1 cut(s) 838
SspI AATATT 1 cut(s) 1951
SspMI CTAG 6 cut(s) 72, 1530, 1826, 2084, 2501, 3000
StyD4I CCNGG 7 cut(s) 392, 481, 537, 928, 1257, 1602, 2953
StyI CCWWGG 1 cut(s) 1492
TaiI ACGT 4 cut(s) 620, 1083, 2850, 2904
TaqI TCGA 6 cut(s) 611, 718, 1168, 1780, 2361, 2425
TaqII GACCGA 1 cut(s) 1520
TatI WGTACW 7 cut(s) 1380, 1472, 1624, 1688, 1805, 2325, 3035
TauI GCSGC 1 cut(s) 876
TfiI GAWTC 7 cut(s) 107, 150, 389, 1067, 1295, 2418, 2450
TseFI GTSAC 3 cut(s) 686, 1007, 1370
Tsp45I GTSAC 3 cut(s) 686, 1007, 1370
TspGWI ACGGA 4 cut(s) 385, 1376, 1578, 2646
Tth111I GACNNNGTC 1 cut(s) 1867
Van91I CCANNNNNTGG 1 cut(s) 310
Vha464I CTTAAG 1 cut(s) 2279
VneI GTGCAC 1 cut(s) 1737
VpaK11BI GGWCC 3 cut(s) 583, 1261, 1640
VspI ATTAAT 1 cut(s) 804
XagI CCTNNNNNAGG 1 cut(s) 2504
XapI RAATTY 6 cut(s) 437, 601, 700, 721, 961, 2222
XceI RCATGY 2 cut(s) 2332, 2380
XhoI CTCGAG 1 cut(s) 2424
XmiI GTMKAC 1 cut(s) 1244
XspI CTAG 6 cut(s) 72, 1530, 1826, 2084, 2501, 3000
ZrmI AGTACT 1 cut(s) 1626
Zsp2I ATGCAT 2 cut(s) 2626, 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.