serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1007 |
| AccB1I | GGYRCC | 1 cut(s) | 928 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 382 |
| AccII | CGCG | 1 cut(s) | 340 |
| AciI | CCGC | 4 cut(s) | 579, 663, 945, 963 |
| AcsI | RAATTY | 6 cut(s) | 469, 584, 702, 733, 760, 816 |
| AcyI | GRCGYC | 1 cut(s) | 929 |
| AfaI | GTAC | 3 cut(s) | 343, 445, 653 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 382, 447, 578 |
| AflIII | ACRYGT | 1 cut(s) | 338 |
| AgsI | TTSAA | 6 cut(s) | 179, 215, 422, 491, 595, 1027 |
| AjiI | CACGTC | 1 cut(s) | 888 |
| AjnI | CCWGG | 2 cut(s) | 514, 1018 |
| AluBI | AGCT | 4 cut(s) | 486, 537, 693, 978 |
| AluI | AGCT | 4 cut(s) | 486, 537, 693, 978 |
| Alw21I | GWGCWC | 1 cut(s) | 113 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 883 |
| ApeKI | GCWGC | 1 cut(s) | 978 |
| ApoI | RAATTY | 6 cut(s) | 469, 584, 702, 733, 760, 816 |
| AspLEI | GCGC | 2 cut(s) | 931, 1008 |
| AsuHPI | GGTGA | 4 cut(s) | 110, 228, 266, 865 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1019, 1052 |
| BanI | GGYRCC | 1 cut(s) | 928 |
| Bbv12I | GWGCWC | 1 cut(s) | 113 |
| BbvI | GCAGC | 1 cut(s) | 965 |
| BccI | CCATC | 3 cut(s) | 83, 359, 514 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 46, 80 |
| BciT130I | CCWGG | 2 cut(s) | 516, 1020 |
| BciVI | GTATCC | 1 cut(s) | 379 |
| BfaI | CTAG | 1 cut(s) | 135 |
| BfmI | CTRYAG | 1 cut(s) | 1061 |
| BfoI | RGCGCY | 1 cut(s) | 932 |
| BfuI | GTATCC | 1 cut(s) | 379 |
| BisI | GCNGC | 2 cut(s) | 964, 979 |
| BlsI | GCNGC | 2 cut(s) | 965, 980 |
| BmcAI | AGTACT | 1 cut(s) | 445 |
| Bme1390I | CCNGG | 2 cut(s) | 516, 1020 |
| BmgBI | CACGTC | 1 cut(s) | 888 |
| BmiI | GGNNCC | 1 cut(s) | 930 |
| BmrFI | CCNGG | 2 cut(s) | 516, 1020 |
| BmrI | ACTGGG | 3 cut(s) | 317, 456, 1027 |
| BmuI | ACTGGG | 3 cut(s) | 317, 456, 1027 |
| BpmI | CTGGAG | 2 cut(s) | 222, 888 |
| BpuEI | CTTGAG | 1 cut(s) | 225 |
| Bsa29I | ATCGAT | 1 cut(s) | 644 |
| BsaHI | GRCGYC | 1 cut(s) | 929 |
| BsaJI | CCNNGG | 2 cut(s) | 287, 515 |
| BsaWI | WCCGGW | 1 cut(s) | 344 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 382, 447, 578 |
| Bse1I | ACTGG | 7 cut(s) | 323, 410, 441, 451, 619, 871, 1033 |
| Bse3DI | GCAATG | 2 cut(s) | 42, 988 |
| BseBI | CCWGG | 2 cut(s) | 516, 1020 |
| BseCI | ATCGAT | 1 cut(s) | 644 |
| BseDI | CCNNGG | 2 cut(s) | 287, 515 |
| BseGI | GGATG | 3 cut(s) | 425, 457, 525 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 382, 447, 578 |
| BseMI | GCAATG | 2 cut(s) | 42, 988 |
| BseMII | CTCAG | 3 cut(s) | 25, 263, 272 |
| BseNI | ACTGG | 7 cut(s) | 323, 410, 441, 451, 619, 871, 1033 |
| BseXI | GCAGC | 1 cut(s) | 965 |
| BseYI | CCCAGC | 1 cut(s) | 571 |
| Bsh1236I | CGCG | 1 cut(s) | 340 |
| BshNI | GGYRCC | 1 cut(s) | 928 |
| BshVI | ATCGAT | 1 cut(s) | 644 |
| BsiHKAI | GWGCWC | 1 cut(s) | 113 |
| BsiSI | CCGG | 1 cut(s) | 345 |
| BslFI | GGGAC | 1 cut(s) | 259 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 382, 447, 578 |
| BsmFI | GGGAC | 1 cut(s) | 259 |
| Bsp1286I | GDGCHC | 1 cut(s) | 113 |
| Bsp143I | GATC | 3 cut(s) | 601, 697, 827 |
| BspACI | CCGC | 4 cut(s) | 579, 663, 945, 963 |
| BspCNI | CTCAG | 3 cut(s) | 24, 262, 273 |
| BspDI | ATCGAT | 1 cut(s) | 644 |
| BspFNI | CGCG | 1 cut(s) | 340 |
| BspLI | GGNNCC | 1 cut(s) | 930 |
| BspT107I | GGYRCC | 1 cut(s) | 928 |
| BsrDI | GCAATG | 2 cut(s) | 42, 988 |
| BsrI | ACTGG | 7 cut(s) | 323, 410, 441, 451, 619, 871, 1033 |
| BssECI | CCNNGG | 2 cut(s) | 287, 515 |
| BssMI | GATC | 3 cut(s) | 601, 697, 827 |
| BssNI | GRCGYC | 1 cut(s) | 929 |
| Bst2UI | CCWGG | 2 cut(s) | 516, 1020 |
| Bst4CI | ACNGT | 4 cut(s) | 51, 131, 295, 879 |
| BstACI | GRCGYC | 1 cut(s) | 929 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 53 |
| BstC8I | GCNNGC | 3 cut(s) | 65, 577, 961 |
| BstDEI | CTNAG | 3 cut(s) | 11, 249, 281 |
| BstEII | GGTNACC | 1 cut(s) | 368 |
| BstF5I | GGATG | 3 cut(s) | 425, 457, 525 |
| BstFNI | CGCG | 1 cut(s) | 340 |
| BstH2I | RGCGCY | 1 cut(s) | 932 |
| BstHHI | GCGC | 2 cut(s) | 931, 1008 |
| BstKTI | GATC | 3 cut(s) | 604, 700, 830 |
| BstMBI | GATC | 3 cut(s) | 601, 697, 827 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 53 |
| BstNI | CCWGG | 2 cut(s) | 516, 1020 |
| BstNSI | RCATGY | 1 cut(s) | 67 |
| BstPI | GGTNACC | 1 cut(s) | 368 |
| BstSCI | CCNGG | 2 cut(s) | 514, 1018 |
| BstSFI | CTRYAG | 1 cut(s) | 1061 |
| BstUI | CGCG | 1 cut(s) | 340 |
| BstV1I | GCAGC | 1 cut(s) | 965 |
| Bsu15I | ATCGAT | 1 cut(s) | 644 |
| BsuI | GTATCC | 1 cut(s) | 379 |
| BsuTUI | ATCGAT | 1 cut(s) | 644 |
| BtrI | CACGTC | 1 cut(s) | 888 |
| BtsCI | GGATG | 3 cut(s) | 425, 457, 525 |
| BtsI | GCAGTG | 2 cut(s) | 471, 914 |
| BtsIMutI | CAGTG | 3 cut(s) | 300, 471, 914 |
| Cac8I | GCNNGC | 3 cut(s) | 65, 577, 961 |
| CaiI | CAGNNNCTG | 1 cut(s) | 883 |
| CfoI | GCGC | 2 cut(s) | 931, 1008 |
| ClaI | ATCGAT | 1 cut(s) | 644 |
| Csp6I | GTAC | 3 cut(s) | 342, 444, 652 |
| CviAII | CATG | 2 cut(s) | 64, 377 |
| CviJI | RGCY | 5 cut(s) | 231, 486, 537, 693, 978 |
| CviKI_1 | RGCY | 5 cut(s) | 231, 486, 537, 693, 978 |
| CviQI | GTAC | 3 cut(s) | 342, 444, 652 |
| DdeI | CTNAG | 3 cut(s) | 11, 249, 281 |
| DinI | GGCGCC | 1 cut(s) | 930 |
| DpnI | GATC | 3 cut(s) | 603, 699, 829 |
| DpnII | GATC | 3 cut(s) | 601, 697, 827 |
| Eco91I | GGTNACC | 1 cut(s) | 368 |
| EcoO65I | GGTNACC | 1 cut(s) | 368 |
| EcoRI | GAATTC | 1 cut(s) | 816 |
| EcoRII | CCWGG | 2 cut(s) | 514, 1018 |
| EgeI | GGCGCC | 1 cut(s) | 930 |
| EheI | GGCGCC | 1 cut(s) | 930 |
| FaeI | CATG | 2 cut(s) | 67, 380 |
| FaqI | GGGAC | 1 cut(s) | 259 |
| FatI | CATG | 2 cut(s) | 63, 376 |
| FauI | CCCGC | 1 cut(s) | 572 |
| FauNDI | CATATG | 1 cut(s) | 266 |
| Fnu4HI | GCNGC | 2 cut(s) | 964, 979 |
| FokI | GGATG | 3 cut(s) | 412, 464, 532 |
| Fsp4HI | GCNGC | 2 cut(s) | 964, 979 |
| FspBI | CTAG | 1 cut(s) | 135 |
| FspI | TGCGCA | 1 cut(s) | 1007 |
| GlaI | GCGC | 2 cut(s) | 930, 1007 |
| GluI | GCNGC | 2 cut(s) | 964, 979 |
| GsaI | CCCAGC | 1 cut(s) | 575 |
| GsuI | CTGGAG | 2 cut(s) | 222, 888 |
| HaeII | RGCGCY | 1 cut(s) | 932 |
| HapII | CCGG | 1 cut(s) | 345 |
| HhaI | GCGC | 2 cut(s) | 931, 1008 |
| Hin1I | GRCGYC | 1 cut(s) | 929 |
| Hin1II | CATG | 2 cut(s) | 67, 380 |
| Hin6I | GCGC | 2 cut(s) | 929, 1006 |
| HinP1I | GCGC | 2 cut(s) | 929, 1006 |
| HincII | GTYRAC | 3 cut(s) | 24, 411, 546 |
| HindII | GTYRAC | 3 cut(s) | 24, 411, 546 |
| HindIII | AAGCTT | 1 cut(s) | 535 |
| HinfI | GANTC | 8 cut(s) | 58, 179, 222, 247, 283, 646, 787, 896 |
| HpaI | GTTAAC | 1 cut(s) | 24 |
| HpaII | CCGG | 1 cut(s) | 345 |
| HphI | GGTGA | 4 cut(s) | 110, 228, 266, 865 |
| Hpy166II | GTNNAC | 3 cut(s) | 24, 411, 546 |
| Hpy188I | TCNGA | 9 cut(s) | 14, 184, 221, 252, 288, 697, 786, 832, 885 |
| Hpy188III | TCNNGA | 1 cut(s) | 201 |
| Hpy8I | GTNNAC | 3 cut(s) | 24, 411, 546 |
| Hpy99I | CGWCG | 1 cut(s) | 173 |
| HpyAV | CCTTC | 1 cut(s) | 243 |
| HpyCH4III | ACNGT | 4 cut(s) | 51, 131, 295, 879 |
| HpyCH4IV | ACGT | 2 cut(s) | 39, 887 |
| HpyCH4V | TGCA | 5 cut(s) | 47, 92, 356, 912, 981 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 53 |
| HpyF3I | CTNAG | 3 cut(s) | 11, 249, 281 |
| HpySE526I | ACGT | 2 cut(s) | 39, 887 |
| Hsp92I | GRCGYC | 1 cut(s) | 929 |
| Hsp92II | CATG | 2 cut(s) | 67, 380 |
| HspAI | GCGC | 2 cut(s) | 929, 1006 |
| KasI | GGCGCC | 1 cut(s) | 928 |
| KspAI | GTTAAC | 1 cut(s) | 24 |
| Kzo9I | GATC | 3 cut(s) | 601, 697, 827 |
| LmnI | GCTCC | 1 cut(s) | 698 |
| Lsp1109I | GCAGC | 1 cut(s) | 965 |
| MaeI | CTAG | 1 cut(s) | 135 |
| MaeII | ACGT | 2 cut(s) | 39, 887 |
| MaeIII | GTNAC | 2 cut(s) | 368, 401 |
| MalI | GATC | 3 cut(s) | 603, 699, 829 |
| MboI | GATC | 3 cut(s) | 601, 697, 827 |
| MboII | GAAGA | 5 cut(s) | 70, 219, 575, 583, 698 |
| MhlI | GDGCHC | 1 cut(s) | 113 |
| MluI | ACGCGT | 1 cut(s) | 338 |
| Mly113I | GGCGCC | 1 cut(s) | 929 |
| MlyI | GAGTC | 3 cut(s) | 241, 292, 796 |
| MmeI | TCCRAC | 3 cut(s) | 363, 521, 589 |
| MseI | TTAA | 3 cut(s) | 23, 437, 954 |
| MspI | CCGG | 1 cut(s) | 345 |
| MspR9I | CCNGG | 2 cut(s) | 516, 1020 |
| MvaI | CCWGG | 2 cut(s) | 516, 1020 |
| MvnI | CGCG | 1 cut(s) | 340 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 53 |
| NarI | GGCGCC | 1 cut(s) | 929 |
| NdeI | CATATG | 1 cut(s) | 266 |
| NdeII | GATC | 3 cut(s) | 601, 697, 827 |
| NlaIII | CATG | 2 cut(s) | 67, 380 |
| NlaIV | GGNNCC | 1 cut(s) | 930 |
| NsbI | TGCGCA | 1 cut(s) | 1007 |
| NspI | RCATGY | 1 cut(s) | 67 |
| PaeI | GCATGC | 1 cut(s) | 67 |
| PfeI | GAWTC | 5 cut(s) | 58, 179, 222, 646, 896 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 382 |
| PkrI | GCNGC | 2 cut(s) | 965, 980 |
| PleI | GAGTC | 3 cut(s) | 241, 291, 795 |
| PluTI | GGCGCC | 1 cut(s) | 932 |
| PpsI | GAGTC | 3 cut(s) | 241, 291, 795 |
| Psp6I | CCWGG | 2 cut(s) | 514, 1018 |
| PspEI | GGTNACC | 1 cut(s) | 368 |
| PspFI | CCCAGC | 1 cut(s) | 571 |
| PspGI | CCWGG | 2 cut(s) | 514, 1018 |
| PspN4I | GGNNCC | 1 cut(s) | 930 |
| PstNI | CAGNNNCTG | 1 cut(s) | 883 |
| RsaI | GTAC | 3 cut(s) | 343, 445, 653 |
| RsaNI | GTAC | 3 cut(s) | 342, 444, 652 |
| SaqAI | TTAA | 3 cut(s) | 23, 437, 954 |
| SatI | GCNGC | 2 cut(s) | 964, 979 |
| Sau3AI | GATC | 3 cut(s) | 601, 697, 827 |
| ScaI | AGTACT | 1 cut(s) | 445 |
| SchI | GAGTC | 3 cut(s) | 241, 292, 796 |
| ScrFI | CCNGG | 2 cut(s) | 516, 1020 |
| SduI | GDGCHC | 1 cut(s) | 113 |
| SfcI | CTRYAG | 1 cut(s) | 1061 |
| SfoI | GGCGCC | 1 cut(s) | 930 |
| SmlI | CTYRAG | 1 cut(s) | 240 |
| SmoI | CTYRAG | 1 cut(s) | 240 |
| SphI | GCATGC | 1 cut(s) | 67 |
| SsiI | CCGC | 4 cut(s) | 579, 663, 945, 963 |
| SspDI | GGCGCC | 1 cut(s) | 928 |
| SspI | AATATT | 1 cut(s) | 994 |
| SspMI | CTAG | 1 cut(s) | 135 |
| StyD4I | CCNGG | 2 cut(s) | 514, 1018 |
| TaaI | ACNGT | 4 cut(s) | 51, 131, 295, 879 |
| TaiI | ACGT | 2 cut(s) | 42, 890 |
| TaqI | TCGA | 1 cut(s) | 644 |
| TatI | WGTACW | 2 cut(s) | 443, 651 |
| TauI | GCSGC | 1 cut(s) | 966 |
| TfiI | GAWTC | 5 cut(s) | 58, 179, 222, 646, 896 |
| Tru1I | TTAA | 3 cut(s) | 23, 437, 954 |
| Tru9I | TTAA | 3 cut(s) | 23, 437, 954 |
| TscAI | CASTG | 3 cut(s) | 300, 471, 914 |
| TseI | GCWGC | 1 cut(s) | 978 |
| TspDTI | ATGAA | 3 cut(s) | 186, 499, 577 |
| TspGWI | ACGGA | 2 cut(s) | 428, 987 |
| TspRI | CASTG | 3 cut(s) | 300, 471, 914 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 382 |
| XapI | RAATTY | 6 cut(s) | 469, 584, 702, 733, 760, 816 |
| XceI | RCATGY | 1 cut(s) | 67 |
| XspI | CTAG | 1 cut(s) | 135 |
| ZrmI | AGTACT | 1 cut(s) | 445 |