MD09G1100100.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000412
Physical Location & Seq
Forward (+)
7333715 .. 7337964
4250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1100100.v1.1.491

Sequence Viewer

Length: 3141 bp
ATGATGTTTCTCAGAGGGAGGTTAACAAGATCAAATTACGTGGTTGCATCGCTTGCGAATCGCATGCTGTTTTCCATCTTCGTTTCGTATGCAGCAGTGTTGTTGTGCTCATCTCAACCATTTTGCTGTGCTAGAGATACAATAACACACGACACTCCAATTACCGACGGTGTTGAATCAGAAACTCTTGTTTCATCTGGTGGAAGATTTGAACTCGGATTCTTTAGCCCTTCACCTGAAGGGACTCAAAGATATGTTGGCATATGGTATCACCAACTGAGTCCGAGGACAGTGGTATGGGTTGCCAATAGAGAAAAGCCAGTTGACAATTCCACTAGTAATGGAAGTCTTGCACTCCAAGATGGTAACCTCCATGTGTTGGATGCTGCTGGAAACAGGTACTGGTCAACGGAGGTTAAAACATCCGAGTCTTTGACGCAGACTGTGACGCTCATGGATTCCGGGAACTTTGTTCTAAGCAGTGGCCATGATAAGTTGGCAGTGAATATTCTGTGGCAGAGCTTTCAAAGTCCAACCGATACATTCATTCCTGGGATGGTAATGGATAAAAGATTGGTGTTGACTTCATGGAGAGGCGAAGATGACCCGAAAAGCGGGCAATTCATCTTCAAAAAAGATCTAGTTAGAGAGGACCAGTATGTCATCTCAAAATCAGAATCGATTCCGTACTGGAAGGGCGGAGAAAGGGCATCTGATACAATTGACAGCTTTGATCAAATGCTTCCTGAAGTTAGTGACTTATTATCAAATTTCAGCAAGAAATCTGTTCGAAATTTCACCAACATTTTCAATTCTCCGAGTCAAAACTCTTCTTATAGAACTAAATATACAAGGTTGGTGATTAATATTACTGGAGAGATACAGTTTTGGAGGTGGATTAATTCCACAAAGCAGTGGAATTTGTATTGGTGGGCGCCAAGAGATAAATGCAGTGTGTTTAATGCTTGCGGCAACTTTGGCAGCTGCAATGATAATAATATGCCGTTTCTGTGCAAATGTTTGCCAGGTTTCAAACCCCAGTATCTACCGCTGTGGAATTCTGGAGACGTTTCGGGTGGATGCACAAGAAAGTCCGCATTATGTGACGACAACAAGAATTATACCTTCTTGAGCTTGAAGAAGATGAAAGTGGGAAACCCAGATTCAGAAAGCAACATTGACAATGAAACAGAATGCAGGAACGGGTGCTTAAATAACTGCCTGTGCAAGGCTTATTCATATGCTAAATCTGAAAGCAATAGTCATCAAAGGGAGGCGCCTACTTCATTGTGCAGAATATGGAACGTGGGAGATCTAAATAATCTTGAAGAAGAGTATACCAATACGGGCCAAGACCTCTATGTTCGTGTTGCGTTGTCTGATTTAGAATCAACTCTAAGAGACTGTAAGCCTTGCGGCACAACCATCATCCCTTATCCCCTGAGCACAAGACTGGGTTGCGGTGACCCTTTGTACTTCCGTTTCAAGTGCAACTACTTCACAGGTCAGGTTAGCTTTGTGGGACTGAATGACGCCTTGTTTAGAGTTATTAGCATAAACTCAAGTACCCAGAGATTTTTCATCCAAGGCTTCCATACCGAAAATGTAGATAACAGTGATCCTAGAAACAGAGCGGCTAAGCAGATCAATCCATCGTTTCCATTTAAAGTAATCAGTCCAGGTAATGCTGACCTGGCTAATTTTAGTTCTGAAGTACTGTTGTCCAGCGGTCCTAATGTAGTGGAGATGGGTTGGAAGCTACCAATGGAACCAAACTGTACAACATCTGCAGACTGCAGGGATTGGGCACATTCAATTTGCAAGCGTGCACGAGGTGGAAAGAAAAGATGCCTGTGCGAAACCAATTTTCGATGGAATGGCTTGATATTAAGATGTACTCAAGAAGGTAATCTTCTAGGGCAACCAAAAGAAGAGCATACGAGAAGAAAATTGCCATTGTCTCTGATAATTGTAGCGGTCCTTTTAAGTGTGATTTTTCTGGCGTGCATCGTCATTTCCATTTATATATGGCGAAGAAATATGAACAACAAACGAGATCAAATAAGACGAGGACAATTTGATAGTGAAAGGAGAGTCAAGGAGTTGATAGACACAAGCGAGTTCAAGGAAGAGGAAGGTATAGATGTACCTTTTTTTGATATGCAAACTATACTAGACGCTACAGATAATTTCTCAAATGCAAACAAGCTTGGACAAGGGGGATACGGGCCTGTTTACAAGGGAATGTTTCTTGGAGGCCAAGAAATCGCGGTGAAAAGGCTATCAAGGGTTTCAGGACAAGGCTTACAGGAATTTAGAAATGAGGTGGTGCTGATCGCCAAACTTCAACACCGAAACCTTGTTAGACTTAAGGGCTATTGCATGAAAGGAGAAGAAAAGATCTTACTCTATGAGTACATGCCTAACAAAAGTTTAGACTACTTTATATTCGATCATACAAAAAGCATGTTTCTCAACTGGGAGATGCGTTTTAGCATCATTTTGGGAATCGCTCGAGGGCTTCTTTATCTTCATCAAGATTCCAGATTGAGGATCATTCATAGAGATTTGAAAACCAGCAACATTCTCCTAGATGAGGAGATGAACCCCAAAATATCTGACTTCGGTTTAGCGAGGATTGTTGGAGGCAAGGAAACTCAGGCAAACACAAACACTGTAGTTGGAACTTATGGTTACATGGCTCCTGAGTATGCATTGGACGGAACTTTCTCAGTGAAATCAGATGTCTTTAGCTTTGGCGTGGTTCTTCTTGAGATAATCAGTGGAAAAAAGAACACAGGCTTTTACCAATCCGAACAAACTTTCAGCCTCATAAATTATGCATGGAGACTCTGGACAGAAAACAAGGTGCTGGATTTAATGGACAAGACTTTGGACGAAAGTTGCAACAAAAACCAGTTTATCCAGTGTGTCAATGTCGGCCTCTTATGCGTACAAGAAGATCCAAACGATCGGCCCGCCATGTCAAATGTTATCACCATGCTCGACAGTGAAACTGCAACCTCTCCGGCTCCTAAACAACCAGGGTTTCTCACAAGGAGAGGCAACTCCAGCACAGCTTCTTCTAGTAAGCCAGAAACTATATCTGAATTAACCACCAGTATAGTAGAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1047

Amino Acids

118.24

Weight (kDa)

8.07

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 97 - 190 4.7e-25 D-mannose binding lectin
S_locus_glycop PF00954 231 - 347 1.3e-20 S-locus glycoprotein domain
PAN_2 PF08276 368 - 439 4.1e-09 PAN-like domain
Pkinase PF00069 732 - 998 1.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 734 - 1001 2.3e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 1004 - 1046 7.1e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 659
AccB1I GGYRCC 2 cut(s) 934, 1276
AccB7I CCANNNNNTGG 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 1634
AccI GTMKAC 1 cut(s) 1337
AccII CGCG 1 cut(s) 2270
AclWI GGATC 3 cut(s) 1613, 2561, 2957
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 5 cut(s) 769, 793, 919, 1057, 2312
AcuI CTGAAG 3 cut(s) 258, 768, 1731
AcyI GRCGYC 3 cut(s) 935, 1277, 1533
AdeI CACNNNGTG 1 cut(s) 1835
AfiI CCNNNNNNNGG 5 cut(s) 379, 614, 1228, 2550, 2596
AflII CTTAAG 1 cut(s) 2369
AhlI ACTAGT 1 cut(s) 335
AjnI CCWGG 5 cut(s) 550, 1024, 1678, 1692, 3043
AluBI AGCT 9 cut(s) 522, 729, 984, 1134, 1515, 1759, 2209, 2754, 3080
AluI AGCT 9 cut(s) 522, 729, 984, 1134, 1515, 1759, 2209, 2754, 3080
Alw21I GWGCWC 3 cut(s) 110, 1448, 1831
Alw26I GTCTC 4 cut(s) 1059, 1395, 1965, 2842
Alw44I GTGCAC 1 cut(s) 1827
AlwI GGATC 3 cut(s) 1613, 2561, 2957
AlwNI CAGNNNCTG 1 cut(s) 402
Ama87I CYCGRG 1 cut(s) 2514
AoxI GGCC 6 cut(s) 484, 1348, 2228, 2257, 2941, 2975
ApaLI GTGCAC 1 cut(s) 1827
ApeKI GCWGC 4 cut(s) 92, 386, 981, 984
ApoI RAATTY 5 cut(s) 769, 793, 919, 1057, 2312
AseI ATTAAT 2 cut(s) 864, 900
Asp700I GAANNNNTTC 1 cut(s) 681
AspLEI GCGC 2 cut(s) 937, 1279
AspS9I GGNCC 6 cut(s) 652, 1348, 1730, 1978, 2228, 2976
AsuC2I CCSGG 1 cut(s) 463
AsuHPI GGTGA 7 cut(s) 225, 263, 790, 871, 1475, 2284, 2989
AsuII TTCGAA 1 cut(s) 790
AvaI CYCGRG 1 cut(s) 2514
AvaII GGWCC 3 cut(s) 652, 1730, 1978
BaeGI GKGCMC 2 cut(s) 1810, 1831
BaeI ACNNNNGTAYC 4 cut(s) 1025, 1058, 1549, 1582
BalI TGGCCA 1 cut(s) 486
BanI GGYRCC 2 cut(s) 934, 1276
BauI CACGAG 1 cut(s) 1830
Bbv12I GWGCWC 3 cut(s) 110, 1448, 1831
BbvI GCAGC 4 cut(s) 104, 373, 971, 993
BccI CCATC 7 cut(s) 83, 356, 550, 1433, 1660, 1741, 1866
BceAI ACGGC 1 cut(s) 988
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 5 cut(s) 552, 1026, 1680, 1694, 3045
BciVI GTATCC 1 cut(s) 2216
BclI TGATCA 1 cut(s) 733
BcnI CCSGG 1 cut(s) 463
BcoDI GTCTC 4 cut(s) 1059, 1395, 1965, 2842
BcuI ACTAGT 1 cut(s) 335
BfaI CTAG 8 cut(s) 132, 336, 641, 1623, 1916, 2174, 2591, 3087
BfmI CTRYAG 4 cut(s) 1788, 1795, 2181, 2676
BfoI RGCGCY 2 cut(s) 938, 1280
BfrI CTTAAG 1 cut(s) 2369
BfuI GTATCC 1 cut(s) 2216
BglII AGATCT 3 cut(s) 637, 1312, 2400
BisI GCNGC 7 cut(s) 93, 387, 970, 982, 985, 1417, 1635
BlpI GCTNAGC 1 cut(s) 1638
BlsI GCNGC 7 cut(s) 94, 388, 971, 983, 986, 1418, 1636
BmcAI AGTACT 1 cut(s) 1716
Bme1390I CCNGG 6 cut(s) 463, 552, 1026, 1680, 1694, 3045
Bme18I GGWCC 3 cut(s) 652, 1730, 1978
BmeT110I CYCGRG 1 cut(s) 2514
BmgT120I GGNCC 6 cut(s) 652, 1348, 1730, 1978, 2228, 2976
BmiI GGNNCC 5 cut(s) 936, 1278, 1770, 2703, 3033
BmrFI CCNGG 6 cut(s) 463, 552, 1026, 1680, 1694, 3045
BmrI ACTGGG 3 cut(s) 1033, 1463, 2488
BmsI GCATC 8 cut(s) 56, 373, 719, 1070, 1838, 2016, 2475, 2505
BmuI ACTGGG 3 cut(s) 1033, 1463, 2488
BpmI CTGGAG 3 cut(s) 894, 1083, 3055
Bpu10I CCTNAGC 1 cut(s) 1442
Bpu1102I GCTNAGC 1 cut(s) 1638
Bpu14I TTCGAA 1 cut(s) 790
BpuEI CTTGAG 4 cut(s) 1150, 1546, 1884, 2792
BpuMI CCSGG 1 cut(s) 463
Bsa29I ATCGAT 1 cut(s) 680
BsaAI YACGTR 1 cut(s) 40
BsaHI GRCGYC 3 cut(s) 935, 1277, 1533
BsaJI CCNNGG 4 cut(s) 284, 551, 1585, 3044
Bsc4I CCNNNNNNNGG 5 cut(s) 379, 614, 1228, 2550, 2596
Bse3DI GCAATG 1 cut(s) 994
BseBI CCWGG 5 cut(s) 552, 1026, 1680, 1694, 3045
BseCI ATCGAT 1 cut(s) 680
BseDI CCNNGG 4 cut(s) 284, 551, 1585, 3044
BseGI GGATG 6 cut(s) 388, 422, 561, 1085, 1428, 1581
BseLI CCNNNNNNNGG 5 cut(s) 379, 614, 1228, 2550, 2596
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 6 cut(s) 25, 269, 1433, 2672, 2697, 2745
BseRI GAGGAG 1 cut(s) 2612
BseSI GKGCMC 2 cut(s) 1810, 1831
BseXI GCAGC 4 cut(s) 104, 373, 971, 993
BsgI GTGCAG 1 cut(s) 1312
Bsh1236I CGCG 1 cut(s) 2270
Bsh1285I CGRYCG 1 cut(s) 2974
BshFI GGCC 6 cut(s) 486, 1350, 2230, 2259, 2943, 2977
BshNI GGYRCC 2 cut(s) 934, 1276
BshVI ATCGAT 1 cut(s) 680
BsiEI CGRYCG 1 cut(s) 2974
BsiHKAI GWGCWC 3 cut(s) 110, 1448, 1831
BsiHKCI CYCGRG 1 cut(s) 2514
BsiSI CCGG 2 cut(s) 462, 3029
BslFI GGGAC 2 cut(s) 256, 1536
BslI CCNNNNNNNGG 5 cut(s) 379, 614, 1228, 2550, 2596
BsmAI GTCTC 4 cut(s) 1059, 1395, 1965, 2842
BsmBI CGTCTC 1 cut(s) 1059
BsmFI GGGAC 2 cut(s) 256, 1536
BsmI GAATGC 1 cut(s) 1199
BsnI GGCC 6 cut(s) 486, 1350, 2230, 2259, 2943, 2977
BsoBI CYCGRG 1 cut(s) 2514
Bsp119I TTCGAA 1 cut(s) 790
Bsp1286I GDGCHC 4 cut(s) 110, 1448, 1810, 1831
Bsp1407I TGTACA 1 cut(s) 1778
Bsp1720I GCTNAGC 1 cut(s) 1638
BspANI GGCC 6 cut(s) 486, 1350, 2230, 2259, 2943, 2977
BspCNI CTCAG 6 cut(s) 24, 270, 1434, 2671, 2698, 2744
BspDI ATCGAT 1 cut(s) 680
BspFNI CGCG 1 cut(s) 2270
BspLI GGNNCC 5 cut(s) 936, 1278, 1770, 2703, 3033
BspMAI CTGCAG 2 cut(s) 1792, 1799
BspPI GGATC 3 cut(s) 1613, 2561, 2957
BspQI GCTCTTC 1 cut(s) 1926
BspT104I TTCGAA 1 cut(s) 790
BspT107I GGYRCC 2 cut(s) 934, 1276
BspTI CTTAAG 1 cut(s) 2369
BsrBI CCGCTC 1 cut(s) 1634
BsrDI GCAATG 1 cut(s) 994
BsrGI TGTACA 1 cut(s) 1778
BssECI CCNNGG 4 cut(s) 284, 551, 1585, 3044
BssNAI GTATAC 1 cut(s) 1338
BssNI GRCGYC 3 cut(s) 935, 1277, 1533
BssSI CACGAG 1 cut(s) 1830
BssT1I CCWWGG 1 cut(s) 1585
Bst1107I GTATAC 1 cut(s) 1338
Bst2BI CACGAG 1 cut(s) 1830
Bst2UI CCWGG 5 cut(s) 552, 1026, 1680, 1694, 3045
Bst6I CTCTTC 4 cut(s) 835, 1326, 1926, 2124
BstACI GRCGYC 3 cut(s) 935, 1277, 1533
BstAFI CTTAAG 1 cut(s) 2369
BstAPI GCANNNNNTGC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 1778
BstBAI YACGTR 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 790
BstC8I GCNNGC 8 cut(s) 54, 65, 617, 967, 1823, 1827, 2005, 2979
BstDEI CTNAG 9 cut(s) 11, 278, 476, 1397, 1442, 1638, 2658, 2706, 2731
BstEII GGTNACC 2 cut(s) 365, 1463
BstENI CCTNNNNNAGG 2 cut(s) 1226, 2594
BstF5I GGATG 6 cut(s) 388, 422, 561, 1085, 1428, 1581
BstFNI CGCG 1 cut(s) 2270
BstH2I RGCGCY 2 cut(s) 938, 1280
BstHHI GCGC 2 cut(s) 937, 1279
BstMAI GTCTC 4 cut(s) 1059, 1395, 1965, 2842
BstMCI CGRYCG 1 cut(s) 2974
BstMWI GCNNNNNNNGC 5 cut(s) 53, 978, 1694, 2949, 3072
BstNI CCWGG 5 cut(s) 552, 1026, 1680, 1694, 3045
BstNSI RCATGY 3 cut(s) 67, 2422, 2470
BstPI GGTNACC 2 cut(s) 365, 1463
BstSCI CCNGG 6 cut(s) 461, 550, 1024, 1678, 1692, 3043
BstSFI CTRYAG 4 cut(s) 1788, 1795, 2181, 2676
BstSLI GKGCMC 2 cut(s) 1810, 1831
BstUI CGCG 1 cut(s) 2270
BstV1I GCAGC 4 cut(s) 104, 373, 971, 993
BstX2I RGATCY 4 cut(s) 637, 1312, 2400, 2962
BstYI RGATCY 4 cut(s) 637, 1312, 2400, 2962
BstZ17I GTATAC 1 cut(s) 1338
Bsu15I ATCGAT 1 cut(s) 680
BsuI GTATCC 1 cut(s) 2216
BsuRI GGCC 6 cut(s) 486, 1350, 2230, 2259, 2943, 2977
BsuTUI ATCGAT 1 cut(s) 680
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 6 cut(s) 388, 422, 561, 1085, 1428, 1581
BtsI GCAGTG 5 cut(s) 102, 487, 507, 920, 958
Cac8I GCNNGC 8 cut(s) 54, 65, 617, 967, 1823, 1827, 2005, 2979
CaiI CAGNNNCTG 1 cut(s) 402
CfoI GCGC 2 cut(s) 937, 1279
Cfr13I GGNCC 6 cut(s) 652, 1348, 1730, 1978, 2228, 2976
ClaI ATCGAT 1 cut(s) 680
CseI GACGC 4 cut(s) 445, 457, 1541, 2186
CspCI CAANNNNNGTGG 2 cut(s) 21, 56
DdeI CTNAG 9 cut(s) 11, 278, 476, 1397, 1442, 1638, 2658, 2706, 2731
DinI GGCGCC 2 cut(s) 936, 1278
DraI TTTAAA 1 cut(s) 1666
DraIII CACNNNGTG 1 cut(s) 1835
DrdI GACNNNNNNGTC 1 cut(s) 659
DseDI GACNNNNNNGTC 1 cut(s) 659
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 4 cut(s) 835, 1326, 1926, 2124
EarI CTCTTC 4 cut(s) 835, 1326, 1926, 2124
EciI GGCGGA 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 1585
Eco47I GGWCC 3 cut(s) 652, 1730, 1978
Eco57I CTGAAG 3 cut(s) 258, 768, 1731
Eco88I CYCGRG 1 cut(s) 2514
Eco91I GGTNACC 2 cut(s) 365, 1463
EcoNI CCTNNNNNAGG 2 cut(s) 1226, 2594
EcoO65I GGTNACC 2 cut(s) 365, 1463
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 5 cut(s) 550, 1024, 1678, 1692, 3043
EcoT14I CCWWGG 1 cut(s) 1585
EcoT22I ATGCAT 2 cut(s) 2716, 2845
EgeI GGCGCC 2 cut(s) 936, 1278
EheI GGCGCC 2 cut(s) 936, 1278
ErhI CCWWGG 1 cut(s) 1585
Esp3I CGTCTC 1 cut(s) 1059
FalI AAGNNNNNCTT 2 cut(s) 1896, 1928
FaqI GGGAC 2 cut(s) 256, 1536
FauI CCCGC 2 cut(s) 608, 2986
FauNDI CATATG 2 cut(s) 263, 1240
FbaI TGATCA 1 cut(s) 733
FblI GTMKAC 1 cut(s) 1337
Fnu4HI GCNGC 7 cut(s) 93, 387, 970, 982, 985, 1417, 1635
FokI GGATG 6 cut(s) 395, 409, 568, 1092, 1415, 1568
Fsp4HI GCNGC 7 cut(s) 93, 387, 970, 982, 985, 1417, 1635
FspBI CTAG 8 cut(s) 132, 336, 641, 1623, 1916, 2174, 2591, 3087
GlaI GCGC 2 cut(s) 936, 1278
GluI GCNGC 7 cut(s) 93, 387, 970, 982, 985, 1417, 1635
GsuI CTGGAG 3 cut(s) 894, 1083, 3055
HaeII RGCGCY 2 cut(s) 938, 1280
HaeIII GGCC 6 cut(s) 486, 1350, 2230, 2259, 2943, 2977
HapII CCGG 2 cut(s) 462, 3029
HgaI GACGC 4 cut(s) 445, 457, 1541, 2186
HhaI GCGC 2 cut(s) 937, 1279
Hin1I GRCGYC 3 cut(s) 935, 1277, 1533
Hin6I GCGC 2 cut(s) 935, 1277
HinP1I GCGC 2 cut(s) 935, 1277
HincII GTYRAC 4 cut(s) 24, 325, 408, 582
HindII GTYRAC 4 cut(s) 24, 325, 408, 582
HindIII AAGCTT 1 cut(s) 2207
HpaI GTTAAC 1 cut(s) 24
HpaII CCGG 2 cut(s) 462, 3029
HphI GGTGA 7 cut(s) 225, 263, 790, 871, 1475, 2284, 2989
Hpy166II GTNNAC 7 cut(s) 24, 325, 408, 582, 1338, 1829, 2236
Hpy8I GTNNAC 7 cut(s) 24, 325, 408, 582, 1338, 1829, 2236
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 7 cut(s) 233, 240, 688, 1135, 1898, 2129, 3125
HpyCH4IV ACGT 3 cut(s) 39, 1068, 1305
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 978, 1694, 2949, 3072
HpyF3I CTNAG 9 cut(s) 11, 278, 476, 1397, 1442, 1638, 2658, 2706, 2731
HpySE526I ACGT 3 cut(s) 39, 1068, 1305
Hsp92I GRCGYC 3 cut(s) 935, 1277, 1533
HspAI GCGC 2 cut(s) 935, 1277
KasI GGCGCC 2 cut(s) 934, 1276
Ksp22I TGATCA 1 cut(s) 733
KspAI GTTAAC 1 cut(s) 24
LguI GCTCTTC 1 cut(s) 1926
LmnI GCTCC 2 cut(s) 2707, 3037
Lsp1109I GCAGC 4 cut(s) 104, 373, 971, 993
LweI GCATC 8 cut(s) 56, 373, 719, 1070, 1838, 2016, 2475, 2505
MaeI CTAG 8 cut(s) 132, 336, 641, 1623, 1916, 2174, 2591, 3087
MaeII ACGT 3 cut(s) 39, 1068, 1305
MaeIII GTNAC 6 cut(s) 365, 445, 755, 1103, 1463, 2693
MbiI CCGCTC 1 cut(s) 1634
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 4 cut(s) 637, 1312, 2400, 2962
MhlI GDGCHC 4 cut(s) 110, 1448, 1810, 1831
MlsI TGGCCA 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 486
Mly113I GGCGCC 2 cut(s) 935, 1277
MlyI GAGTC 6 cut(s) 238, 289, 437, 829, 2103, 2844
MmeI TCCRAC 5 cut(s) 360, 557, 1733, 2623, 2662
Mox20I TGGCCA 1 cut(s) 486
Mph1103I ATGCAT 2 cut(s) 2716, 2845
MroXI GAANNNNTTC 1 cut(s) 681
MscI TGGCCA 1 cut(s) 486
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 3 cut(s) 984, 1051, 1728
MspCI CTTAAG 1 cut(s) 2369
MspI CCGG 2 cut(s) 462, 3029
MspR9I CCNGG 6 cut(s) 463, 552, 1026, 1680, 1694, 3045
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 1 cut(s) 1199
MvaI CCWGG 5 cut(s) 552, 1026, 1680, 1694, 3045
MvnI CGCG 1 cut(s) 2270
MwoI GCNNNNNNNGC 5 cut(s) 53, 978, 1694, 2949, 3072
NarI GGCGCC 2 cut(s) 935, 1277
NciI CCSGG 1 cut(s) 463
NdeI CATATG 2 cut(s) 263, 1240
NlaIV GGNNCC 5 cut(s) 936, 1278, 1770, 2703, 3033
NmuCI GTSAC 4 cut(s) 445, 755, 1103, 1463
NsiI ATGCAT 2 cut(s) 2716, 2845
NspI RCATGY 3 cut(s) 67, 2422, 2470
NspV TTCGAA 1 cut(s) 790
PaeI GCATGC 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 2514
PciSI GCTCTTC 1 cut(s) 1926
PctI GAATGC 1 cut(s) 1199
PdmI GAANNNNTTC 1 cut(s) 681
PflMI CCANNNNNTGG 1 cut(s) 379
PfoI TCCNGGA 1 cut(s) 461
PkrI GCNGC 7 cut(s) 94, 388, 971, 983, 986, 1418, 1636
Ple19I CGATCG 1 cut(s) 2974
PleI GAGTC 6 cut(s) 238, 288, 436, 828, 2102, 2844
PluTI GGCGCC 2 cut(s) 938, 1280
PpsI GAGTC 6 cut(s) 238, 288, 436, 828, 2102, 2844
Ppu21I YACGTR 1 cut(s) 40
PshBI ATTAAT 2 cut(s) 864, 900
Psp6I CCWGG 5 cut(s) 550, 1024, 1678, 1692, 3043
PspEI GGTNACC 2 cut(s) 365, 1463
PspGI CCWGG 5 cut(s) 550, 1024, 1678, 1692, 3043
PspN4I GGNNCC 5 cut(s) 936, 1278, 1770, 2703, 3033
PspPI GGNCC 6 cut(s) 652, 1348, 1730, 1978, 2228, 2976
PspXI VCTCGAGB 1 cut(s) 2514
PsrI GAACNNNNNNTAC 2 cut(s) 832, 864
PstI CTGCAG 2 cut(s) 1792, 1799
PstNI CAGNNNCTG 1 cut(s) 402
PsuI RGATCY 4 cut(s) 637, 1312, 2400, 2962
PvuI CGATCG 1 cut(s) 2974
PvuII CAGCTG 1 cut(s) 984
SapI GCTCTTC 1 cut(s) 1926
SatI GCNGC 7 cut(s) 93, 387, 970, 982, 985, 1417, 1635
Sau96I GGNCC 6 cut(s) 652, 1348, 1730, 1978, 2228, 2976
ScaI AGTACT 1 cut(s) 1716
SchI GAGTC 6 cut(s) 238, 289, 437, 829, 2103, 2844
ScrFI CCNGG 6 cut(s) 463, 552, 1026, 1680, 1694, 3045
SduI GDGCHC 4 cut(s) 110, 1448, 1810, 1831
SfaNI GCATC 8 cut(s) 56, 373, 719, 1070, 1838, 2016, 2475, 2505
SfcI CTRYAG 4 cut(s) 1788, 1795, 2181, 2676
SfoI GGCGCC 2 cut(s) 936, 1278
Sfr274I CTCGAG 1 cut(s) 2514
SfuI TTCGAA 1 cut(s) 790
SinI GGWCC 3 cut(s) 652, 1730, 1978
SlaI CTCGAG 1 cut(s) 2514
SmlI CTYRAG 6 cut(s) 1129, 1561, 1899, 2369, 2514, 2771
SmoI CTYRAG 6 cut(s) 1129, 1561, 1899, 2369, 2514, 2771
SpeI ACTAGT 1 cut(s) 335
SphI GCATGC 1 cut(s) 67
SspDI GGCGCC 2 cut(s) 934, 1276
SspI AATATT 2 cut(s) 508, 868
SspMI CTAG 8 cut(s) 132, 336, 641, 1623, 1916, 2174, 2591, 3087
StyD4I CCNGG 6 cut(s) 461, 550, 1024, 1678, 1692, 3043
StyI CCWWGG 1 cut(s) 1585
TaiI ACGT 3 cut(s) 42, 1071, 1308
TaqI TCGA 6 cut(s) 680, 790, 1870, 2451, 2515, 3006
TatI WGTACW 5 cut(s) 1473, 1714, 1778, 1895, 2415
TauI GCSGC 3 cut(s) 972, 1419, 1637
TseFI GTSAC 4 cut(s) 445, 755, 1103, 1463
TseI GCWGC 4 cut(s) 92, 386, 981, 984
Tsp45I GTSAC 4 cut(s) 445, 755, 1103, 1463
TspGWI ACGGA 4 cut(s) 425, 675, 1469, 2736
Van91I CCANNNNNTGG 1 cut(s) 379
Vha464I CTTAAG 1 cut(s) 2369
VneI GTGCAC 1 cut(s) 1827
VpaK11BI GGWCC 3 cut(s) 652, 1730, 1978
VspI ATTAAT 2 cut(s) 864, 900
XagI CCTNNNNNAGG 2 cut(s) 1226, 2594
XapI RAATTY 5 cut(s) 769, 793, 919, 1057, 2312
XceI RCATGY 3 cut(s) 67, 2422, 2470
XhoI CTCGAG 1 cut(s) 2514
XmiI GTMKAC 1 cut(s) 1337
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 8 cut(s) 132, 336, 641, 1623, 1916, 2174, 2591, 3087
ZrmI AGTACT 1 cut(s) 1716
Zsp2I ATGCAT 2 cut(s) 2716, 2845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.