MD09G1100000.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000412
Physical Location & Seq
Forward (+)
7319236 .. 7323098
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1100000.v1.1.491

Sequence Viewer

Length: 2421 bp
ATGATGTTTCTCAGAGGGAGGTTAACAAGATCAAATTACGTCATTGCATCGTTTGTGAATCGCATGCTGTTTTACATATTTTTTTCGTATGCAGCAGTCTTCTTGTGCTCATCTCACCCATTTTGCTGTGCTAGAGATACAATAACATATGGCGCTCCAATTACCGACGGTGTTGAATCAGGAACTCTTGTTTCATCTGGTGGAAGATTTGAACTCGGATTCTTTAGCCCTTCACTTAATGGGACTCAAAGATATGTTGGCATATGGTATCACCAACTGAGTCCGAGGACAGTGGTATGGGTTGCCAACAGAGAAACCCCAGTTCTCAATTCCACTAGAACCGGATTTCTTGCACTCCAAGATGGGAACCTCCATGTGTTGGATGCTGCTGGAAAGAGGTACTGGTCAGCGGAGGGTGAAACATCCAAGTCTTTGACGCAGACTGTGACGCTCATGGATTCCGGGAACCTTCTTTCAAAGCCAACTGATACATTCATTCCTGGGATGGTAATGGATAAAATCTTGGAGTTGTCTTCATGGAGAGGCGAAGATGACCCGGCAAGCGGGCAATTCATCTTCAAACAAGATCCAGTTGGAGAGAACCAGTATGTCATCACAAAATCAGAATCGATTCTGTACTGGAAAGGCGGAGAAAGGGCATCTGATACATTTTACAGCTTTGATCAAATGCTTCCTGCAGTTACTTACTTATTATCAAATTTCAGCAAGAAATATGTTGTTCGAAATTCCACCAACATTTTCAATTCTCCGAGTCAGAACTCTTCTTATAAAAATATAGAAATTCTGCCACAATCAGAATACAAATATACAAGGTTGGTGATTAGTATTACTGGAGAGATACAGTTTTGGACGTGGATTAATTCCACAAAGCGGTGGAAATTGTATTGGTCGGCGCCAAGAGATAAATGCAGTGTGTTTAATGCTTGCGGCAACTATGGCAGCTGCAATGATAATAATATGCCGTTTCTGTGCAAATGTTTGCCAGGTTTCAAACCCCAGTATCTACCGCAGTGGAATTCTGGAGACTTTTCAGGTGGATGCACAAGAAAGTCCTTATGTGTTGACAGCAAGAATGATACCTTCTTGAGCTTGAAGAAGATGAAAGTGGGAAACCCAGATTCACAAATCAACGTTGACAATGAAACAGTATGCTGGAACCAGTGCTTAAATAGCTGCCAGTGTAAGGCTTATTCATATGCTAAATCTGAAAACTATAGTCATCGAAGGGATGCGCATGCGCCTACTTCATTGTGCAGAATATGGAACGAGGGAGATCTAAATAATCTTGAAGAAGAGTATACTGATGGGGGTCAGGACCTCTCTGTTCGTGTTGCATTGTCTGATTTAGTACTGTCATCCAGAGGTCCTAATGTAATGGCGATGGGTTGGGAACTACCAATGGAACCAACCTGTACAACACCCGCAGACTGCAGGGATTGGCCACATTCAATTTGCAATCCTGCAAGAGACAGAAAGAAAAGATGCCTTTGCAAAACCAATTTTCGATGGGATGGCTTGAATTGTACTCAAGATCAAATAAGACGAGGACAATTTGATAGTGAAAGGAGAGTCAAGGAGTTGATAGACACAAGCGAGTTCAAGGAAGAGGAAGGTATAGATGTACCTTTTTTTGATATGCAAACTATACTAGACGCAACAGATAATTTCTCAAATGCAAACAAGCTTGGACAAGGGGGATACGGGCCTGTTTACAAGGGAGTGTTTCTTGGAGGCCAAGAAATCGCGGTGAAAAGGCTATCAAGGGTGTCAGGACAAGGCTTACAGGAATTTAGAAATGAGGTGGTGCTGATCGCCAAACTTCAACACCGAAACCTTGTTAGACTTAAGGGCTATTGCATGAAAGGAGAAGAAAAGATCTTACTCTATGACTACATGCCTAACAAAAGTTTAGACTCCTTTATATTCGCTACTAAATTTTTCTCTAAATGGAAAATGGAAATTCTGCAAGATCAAACAAAAAGCATGTTTCTCAACTGGGAGATGCGTTTTAGCATCATTTTGGGAATCGCTCGAGGGCTTCTTTATCTTCATCAAGATTCCAGATTGAGGATCATTCATAGAGATTTAAAAACCAGCAACATTCTCCTAGATGAGGAGATGAACCCCAAAATATCCGACTTCGGTTTAGCGAGGATTGTTGGAGGCAAGGAAACTCAGGCAAACACAAACACTGTAGTTGGAACTTATGGTTACATGGCTCCGGAGTATGCATTGGACGGAACTTTCTCAGTGAAATCAGATGTCTTTAGCTTTGGCGTGGTTCTTCTTGACATAATCAGCGGAAAAAAGAACACAGGCTTTTACCAATCCGAACAAACTTTCAGCCTCATAAATTATGTAAGACAGCAAGTGCGTCGAATATTTCATATCCATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

807

Amino Acids

91.8

Weight (kDa)

8.45

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 97 - 158 2e-12 D-mannose binding lectin
S_locus_glycop PF00954 254 - 340 3.5e-20 S-locus glycoprotein domain
PAN_2 PF08276 360 - 430 5.5e-09 PAN-like domain
Pkinase PF00069 564 - 787 2.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 566 - 796 1.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
Acc16I TGCGCA 1 cut(s) 1254
AccB1I GGYRCC 1 cut(s) 913
AccB7I CCANNNNNTGG 1 cut(s) 379
AccI GTMKAC 1 cut(s) 1319
AccII CGCG 1 cut(s) 1766
AccIII TCCGGA 1 cut(s) 2242
AciI CCGC 9 cut(s) 410, 564, 648, 892, 948, 1028, 1443, 1766, 2322
AclI AACGTT 1 cut(s) 1152
AclWI GGATC 2 cut(s) 581, 2099
AcoI YGGCCR 1 cut(s) 1460
AcsI RAATTY 7 cut(s) 718, 745, 801, 1036, 1808, 1955, 1980
AcyI GRCGYC 1 cut(s) 914
AfaI GTAC 6 cut(s) 401, 638, 1371, 1435, 1546, 1644
AfiI CCNNNNNNNGG 6 cut(s) 379, 563, 891, 1204, 2088, 2134
AflII CTTAAG 1 cut(s) 1865
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 2 cut(s) 499, 1003
AluBI AGCT 6 cut(s) 678, 963, 1110, 1194, 1705, 2292
AluI AGCT 6 cut(s) 678, 963, 1110, 1194, 1705, 2292
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 1038, 1482
AlwI GGATC 2 cut(s) 581, 2099
Ama87I CYCGRG 1 cut(s) 2052
Aor13HI TCCGGA 1 cut(s) 2242
AoxI GGCC 3 cut(s) 1460, 1724, 1753
ApeKI GCWGC 5 cut(s) 92, 386, 960, 963, 1194
ApoI RAATTY 7 cut(s) 718, 745, 801, 1036, 1808, 1955, 1980
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 1 cut(s) 630
AspLEI GCGC 4 cut(s) 155, 916, 1255, 1261
AspS9I GGNCC 3 cut(s) 1336, 1385, 1724
AsuC2I CCSGG 2 cut(s) 463, 557
AsuHPI GGTGA 5 cut(s) 107, 263, 428, 850, 1780
AsuII TTCGAA 1 cut(s) 742
AvaI CYCGRG 1 cut(s) 2052
AvaII GGWCC 2 cut(s) 1336, 1385
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BalI TGGCCA 1 cut(s) 1462
BanI GGYRCC 1 cut(s) 913
BbsI GAAGAC 2 cut(s) 91, 525
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 5 cut(s) 104, 373, 950, 972, 1181
BccI CCATC 6 cut(s) 356, 499, 1319, 1396, 1521, 1526
BceAI ACGGC 1 cut(s) 967
BcgI CGANNNNNNTGC 2 cut(s) 2378, 2412
BciT130I CCWGG 2 cut(s) 501, 1005
BciVI GTATCC 1 cut(s) 1712
BclI TGATCA 1 cut(s) 682
BcnI CCSGG 2 cut(s) 463, 557
BcoDI GTCTC 2 cut(s) 1038, 1482
BfaI CTAG 4 cut(s) 132, 336, 1670, 2129
BfmI CTRYAG 4 cut(s) 696, 1234, 1450, 2214
BfoI RGCGCY 2 cut(s) 156, 917
BfrI CTTAAG 1 cut(s) 1865
BfuI GTATCC 1 cut(s) 1712
BglII AGATCT 2 cut(s) 1294, 1896
BisI GCNGC 6 cut(s) 93, 387, 949, 961, 964, 1195
BlsI GCNGC 6 cut(s) 94, 388, 950, 962, 965, 1196
BmcAI AGTACT 1 cut(s) 1371
Bme1390I CCNGG 4 cut(s) 463, 501, 557, 1005
Bme18I GGWCC 2 cut(s) 1336, 1385
BmeT110I CYCGRG 1 cut(s) 2052
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 3 cut(s) 1336, 1385, 1724
BmiI GGNNCC 6 cut(s) 368, 467, 915, 1178, 1425, 2241
BmrFI CCNGG 4 cut(s) 463, 501, 557, 1005
BmrI ACTGGG 3 cut(s) 314, 1012, 2026
BmsI GCATC 8 cut(s) 56, 373, 668, 1049, 1240, 1493, 2013, 2043
BmuI ACTGGG 3 cut(s) 314, 1012, 2026
BpiI GAAGAC 2 cut(s) 91, 525
BpmI CTGGAG 2 cut(s) 873, 1062
Bpu14I TTCGAA 1 cut(s) 742
BpuEI CTTGAG 2 cut(s) 1126, 1533
BpuMI CCSGG 2 cut(s) 463, 557
Bsa29I ATCGAT 1 cut(s) 629
BsaHI GRCGYC 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 284, 500
BsaWI WCCGGW 2 cut(s) 341, 2242
Bsc4I CCNNNNNNNGG 6 cut(s) 379, 563, 891, 1204, 2088, 2134
Bse3DI GCAATG 2 cut(s) 42, 973
BseAI TCCGGA 1 cut(s) 2242
BseBI CCWGG 2 cut(s) 501, 1005
BseCI ATCGAT 1 cut(s) 629
BseDI CCNNGG 2 cut(s) 284, 500
BseGI GGATG 7 cut(s) 388, 422, 510, 1064, 1255, 1376, 1537
BseLI CCNNNNNNNGG 6 cut(s) 379, 563, 891, 1204, 2088, 2134
BseMI GCAATG 2 cut(s) 42, 973
BseMII CTCAG 4 cut(s) 25, 269, 2210, 2283
BseRI GAGGAG 1 cut(s) 2150
BseXI GCAGC 5 cut(s) 104, 373, 950, 972, 1181
BsgI GTGCAG 1 cut(s) 1294
Bsh1236I CGCG 1 cut(s) 1766
BshFI GGCC 3 cut(s) 1462, 1726, 1755
BshNI GGYRCC 1 cut(s) 913
BshVI ATCGAT 1 cut(s) 629
BsiHKAI GWGCWC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 2052
BsiSI CCGG 4 cut(s) 342, 462, 557, 2243
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 6 cut(s) 379, 563, 891, 1204, 2088, 2134
BsmAI GTCTC 2 cut(s) 1038, 1482
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 1462, 1726, 1755
BsoBI CYCGRG 1 cut(s) 2052
Bsp119I TTCGAA 1 cut(s) 742
Bsp1286I GDGCHC 1 cut(s) 110
Bsp13I TCCGGA 1 cut(s) 2242
Bsp1407I TGTACA 1 cut(s) 1433
Bsp143I GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
BspACI CCGC 9 cut(s) 410, 564, 648, 892, 948, 1028, 1443, 1766, 2322
BspANI GGCC 3 cut(s) 1462, 1726, 1755
BspCNI CTCAG 4 cut(s) 24, 270, 2209, 2282
BspDI ATCGAT 1 cut(s) 629
BspEI TCCGGA 1 cut(s) 2242
BspFNI CGCG 1 cut(s) 1766
BspLI GGNNCC 6 cut(s) 368, 467, 915, 1178, 1425, 2241
BspMAI CTGCAG 2 cut(s) 700, 1454
BspPI GGATC 2 cut(s) 581, 2099
BspT104I TTCGAA 1 cut(s) 742
BspT107I GGYRCC 1 cut(s) 913
BspTI CTTAAG 1 cut(s) 1865
BsrDI GCAATG 2 cut(s) 42, 973
BsrGI TGTACA 1 cut(s) 1433
BssECI CCNNGG 2 cut(s) 284, 500
BssMI GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
BssNAI GTATAC 1 cut(s) 1320
BssNI GRCGYC 1 cut(s) 914
Bst1107I GTATAC 1 cut(s) 1320
Bst2UI CCWGG 2 cut(s) 501, 1005
Bst4CI ACNGT 7 cut(s) 170, 292, 445, 864, 1168, 1374, 2215
Bst6I CTCTTC 3 cut(s) 787, 1308, 1620
BstACI GRCGYC 1 cut(s) 914
BstAFI CTTAAG 1 cut(s) 1865
BstAUI TGTACA 1 cut(s) 1433
BstBI TTCGAA 1 cut(s) 742
BstC8I GCNNGC 5 cut(s) 65, 562, 566, 946, 1257
BstDEI CTNAG 4 cut(s) 11, 278, 2196, 2269
BstENI CCTNNNNNAGG 1 cut(s) 2132
BstF5I GGATG 7 cut(s) 388, 422, 510, 1064, 1255, 1376, 1537
BstFNI CGCG 1 cut(s) 1766
BstH2I RGCGCY 2 cut(s) 156, 917
BstHHI GCGC 4 cut(s) 155, 916, 1255, 1261
BstKTI GATC 9 cut(s) 32, 589, 685, 1297, 1555, 1833, 1899, 1993, 2094
BstMAI GTCTC 2 cut(s) 1038, 1482
BstMBI GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
BstMWI GCNNNNNNNGC 2 cut(s) 957, 1191
BstNI CCWGG 2 cut(s) 501, 1005
BstNSI RCATGY 4 cut(s) 67, 1259, 1918, 2008
BstSCI CCNGG 4 cut(s) 461, 499, 555, 1003
BstSFI CTRYAG 4 cut(s) 696, 1234, 1450, 2214
BstUI CGCG 1 cut(s) 1766
BstV1I GCAGC 5 cut(s) 104, 373, 950, 972, 1181
BstV2I GAAGAC 2 cut(s) 91, 525
BstX2I RGATCY 3 cut(s) 586, 1294, 1896
BstYI RGATCY 3 cut(s) 586, 1294, 1896
BstZ17I GTATAC 1 cut(s) 1320
Bsu15I ATCGAT 1 cut(s) 629
BsuI GTATCC 1 cut(s) 1712
BsuRI GGCC 3 cut(s) 1462, 1726, 1755
BsuTUI ATCGAT 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 1415
BtrI CACGTC 1 cut(s) 873
BtsCI GGATG 7 cut(s) 388, 422, 510, 1064, 1255, 1376, 1537
BtsI GCAGTG 2 cut(s) 937, 1037
BtsIMutI CAGTG 7 cut(s) 297, 937, 1037, 1187, 1205, 2211, 2277
Cac8I GCNNGC 5 cut(s) 65, 562, 566, 946, 1257
CfoI GCGC 4 cut(s) 155, 916, 1255, 1261
Cfr13I GGNCC 3 cut(s) 1336, 1385, 1724
ClaI ATCGAT 1 cut(s) 629
CseI GACGC 4 cut(s) 445, 457, 1682, 2384
Csp6I GTAC 6 cut(s) 400, 637, 1370, 1434, 1545, 1643
CviAII CATG 9 cut(s) 64, 374, 454, 537, 1256, 1879, 1915, 2005, 2236
CviQI GTAC 6 cut(s) 400, 637, 1370, 1434, 1545, 1643
DdeI CTNAG 4 cut(s) 11, 278, 2196, 2269
DinI GGCGCC 1 cut(s) 915
DpnI GATC 9 cut(s) 31, 588, 684, 1296, 1554, 1832, 1898, 1992, 2093
DpnII GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
DraI TTTAAA 1 cut(s) 2109
EaeI YGGCCR 1 cut(s) 1460
Eam1104I CTCTTC 3 cut(s) 787, 1308, 1620
EarI CTCTTC 3 cut(s) 787, 1308, 1620
EciI GGCGGA 1 cut(s) 663
Eco47I GGWCC 2 cut(s) 1336, 1385
Eco88I CYCGRG 1 cut(s) 2052
EcoNI CCTNNNNNAGG 1 cut(s) 2132
EcoO109I RGGNCCY 2 cut(s) 1336, 1385
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 2 cut(s) 499, 1003
EcoT22I ATGCAT 1 cut(s) 2254
EgeI GGCGCC 1 cut(s) 915
EheI GGCGCC 1 cut(s) 915
FaeI CATG 9 cut(s) 67, 377, 457, 540, 1259, 1882, 1918, 2008, 2239
FaqI GGGAC 1 cut(s) 256
FatI CATG 9 cut(s) 63, 373, 453, 536, 1255, 1878, 1914, 2004, 2235
FauI CCCGC 2 cut(s) 557, 1450
FauNDI CATATG 3 cut(s) 148, 263, 1216
FbaI TGATCA 1 cut(s) 682
FblI GTMKAC 1 cut(s) 1319
Fnu4HI GCNGC 6 cut(s) 93, 387, 949, 961, 964, 1195
FokI GGATG 7 cut(s) 395, 409, 517, 1071, 1262, 1363, 1544
Fsp4HI GCNGC 6 cut(s) 93, 387, 949, 961, 964, 1195
FspAI RTGCGCAY 1 cut(s) 1254
FspBI CTAG 4 cut(s) 132, 336, 1670, 2129
FspI TGCGCA 1 cut(s) 1254
GlaI GCGC 4 cut(s) 154, 915, 1254, 1260
GluI GCNGC 6 cut(s) 93, 387, 949, 961, 964, 1195
GsuI CTGGAG 2 cut(s) 873, 1062
HaeII RGCGCY 2 cut(s) 156, 917
HaeIII GGCC 3 cut(s) 1462, 1726, 1755
HapII CCGG 4 cut(s) 342, 462, 557, 2243
HgaI GACGC 4 cut(s) 445, 457, 1682, 2384
HhaI GCGC 4 cut(s) 155, 916, 1255, 1261
Hin1I GRCGYC 1 cut(s) 914
Hin1II CATG 9 cut(s) 67, 377, 457, 540, 1259, 1882, 1918, 2008, 2239
Hin6I GCGC 4 cut(s) 153, 914, 1253, 1259
HinP1I GCGC 4 cut(s) 153, 914, 1253, 1259
HincII GTYRAC 3 cut(s) 24, 1084, 1156
HindII GTYRAC 3 cut(s) 24, 1084, 1156
HindIII AAGCTT 1 cut(s) 1703
HpaI GTTAAC 1 cut(s) 24
HpaII CCGG 4 cut(s) 342, 462, 557, 2243
HphI GGTGA 5 cut(s) 107, 263, 428, 850, 1780
Hpy166II GTNNAC 5 cut(s) 24, 1084, 1156, 1320, 1732
Hpy8I GTNNAC 5 cut(s) 24, 1084, 1156, 1320, 1732
Hpy99I CGWCG 2 cut(s) 170, 2400
HpyAV CCTTC 5 cut(s) 240, 479, 1111, 1239, 1625
HpyCH4III ACNGT 7 cut(s) 170, 292, 445, 864, 1168, 1374, 2215
HpyCH4IV ACGT 3 cut(s) 39, 872, 1152
HpyF10VI GCNNNNNNNGC 2 cut(s) 957, 1191
HpyF3I CTNAG 4 cut(s) 11, 278, 2196, 2269
HpySE526I ACGT 3 cut(s) 39, 872, 1152
Hsp92I GRCGYC 1 cut(s) 914
Hsp92II CATG 9 cut(s) 67, 377, 457, 540, 1259, 1882, 1918, 2008, 2239
HspAI GCGC 4 cut(s) 153, 914, 1253, 1259
KasI GGCGCC 1 cut(s) 913
Kpn2I TCCGGA 1 cut(s) 2242
Ksp22I TGATCA 1 cut(s) 682
KspAI GTTAAC 1 cut(s) 24
Kzo9I GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
LmnI GCTCC 2 cut(s) 160, 2245
Lsp1109I GCAGC 5 cut(s) 104, 373, 950, 972, 1181
LweI GCATC 8 cut(s) 56, 373, 668, 1049, 1240, 1493, 2013, 2043
MaeI CTAG 4 cut(s) 132, 336, 1670, 2129
MaeII ACGT 3 cut(s) 39, 872, 1152
MaeIII GTNAC 3 cut(s) 445, 700, 2231
MalI GATC 9 cut(s) 31, 588, 684, 1296, 1554, 1832, 1898, 1992, 2093
MboI GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
MflI RGATCY 3 cut(s) 586, 1294, 1896
MhlI GDGCHC 1 cut(s) 110
MlsI TGGCCA 1 cut(s) 1462
MluNI TGGCCA 1 cut(s) 1462
Mly113I GGCGCC 1 cut(s) 914
MlyI GAGTC 5 cut(s) 238, 289, 781, 1599, 1928
MmeI TCCRAC 5 cut(s) 360, 574, 2161, 2181, 2200
Mox20I TGGCCA 1 cut(s) 1462
Mph1103I ATGCAT 1 cut(s) 2254
MroI TCCGGA 1 cut(s) 2242
MroXI GAANNNNTTC 1 cut(s) 630
MscI TGGCCA 1 cut(s) 1462
MseI TTAA 8 cut(s) 23, 237, 879, 939, 1187, 1866, 2108, 2419
Msp20I TGGCCA 1 cut(s) 1462
MspA1I CMGCKG 3 cut(s) 410, 963, 2322
MspCI CTTAAG 1 cut(s) 1865
MspI CCGG 4 cut(s) 342, 462, 557, 2243
MspR9I CCNGG 4 cut(s) 463, 501, 557, 1005
MvaI CCWGG 2 cut(s) 501, 1005
MvnI CGCG 1 cut(s) 1766
MwoI GCNNNNNNNGC 2 cut(s) 957, 1191
NarI GGCGCC 1 cut(s) 914
NciI CCSGG 2 cut(s) 463, 557
NdeI CATATG 3 cut(s) 148, 263, 1216
NdeII GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
NlaIII CATG 9 cut(s) 67, 377, 457, 540, 1259, 1882, 1918, 2008, 2239
NlaIV GGNNCC 6 cut(s) 368, 467, 915, 1178, 1425, 2241
NmuCI GTSAC 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 1254
NsiI ATGCAT 1 cut(s) 2254
NspI RCATGY 4 cut(s) 67, 1259, 1918, 2008
NspV TTCGAA 1 cut(s) 742
PaeI GCATGC 2 cut(s) 67, 1259
PaeR7I CTCGAG 1 cut(s) 2052
PdmI GAANNNNTTC 1 cut(s) 630
PfeI GAWTC 9 cut(s) 58, 176, 219, 458, 626, 631, 1139, 2046, 2078
PflMI CCANNNNNTGG 1 cut(s) 379
PfoI TCCNGGA 1 cut(s) 461
PkrI GCNGC 6 cut(s) 94, 388, 950, 962, 965, 1196
PleI GAGTC 5 cut(s) 238, 288, 780, 1598, 1928
PluTI GGCGCC 1 cut(s) 917
PpsI GAGTC 5 cut(s) 238, 288, 780, 1598, 1928
PpuMI RGGWCCY 2 cut(s) 1336, 1385
PshBI ATTAAT 1 cut(s) 879
PsiI TTATAA 1 cut(s) 789
Psp1406I AACGTT 1 cut(s) 1152
Psp5II RGGWCCY 2 cut(s) 1336, 1385
Psp6I CCWGG 2 cut(s) 499, 1003
PspGI CCWGG 2 cut(s) 499, 1003
PspN4I GGNNCC 6 cut(s) 368, 467, 915, 1178, 1425, 2241
PspPI GGNCC 3 cut(s) 1336, 1385, 1724
PspPPI RGGWCCY 2 cut(s) 1336, 1385
PspXI VCTCGAGB 1 cut(s) 2052
PstI CTGCAG 2 cut(s) 700, 1454
PsuI RGATCY 3 cut(s) 586, 1294, 1896
PvuII CAGCTG 1 cut(s) 963
RsaI GTAC 6 cut(s) 401, 638, 1371, 1435, 1546, 1644
RsaNI GTAC 6 cut(s) 400, 637, 1370, 1434, 1545, 1643
SaqAI TTAA 8 cut(s) 23, 237, 879, 939, 1187, 1866, 2108, 2419
SatI GCNGC 6 cut(s) 93, 387, 949, 961, 964, 1195
Sau3AI GATC 9 cut(s) 29, 586, 682, 1294, 1552, 1830, 1896, 1990, 2091
Sau96I GGNCC 3 cut(s) 1336, 1385, 1724
ScaI AGTACT 1 cut(s) 1371
SchI GAGTC 5 cut(s) 238, 289, 781, 1599, 1928
ScrFI CCNGG 4 cut(s) 463, 501, 557, 1005
SduI GDGCHC 1 cut(s) 110
SfaNI GCATC 8 cut(s) 56, 373, 668, 1049, 1240, 1493, 2013, 2043
SfcI CTRYAG 4 cut(s) 696, 1234, 1450, 2214
SfoI GGCGCC 1 cut(s) 915
Sfr274I CTCGAG 1 cut(s) 2052
SfuI TTCGAA 1 cut(s) 742
SinI GGWCC 2 cut(s) 1336, 1385
SlaI CTCGAG 1 cut(s) 2052
SmlI CTYRAG 4 cut(s) 1105, 1548, 1865, 2052
SmoI CTYRAG 4 cut(s) 1105, 1548, 1865, 2052
SphI GCATGC 2 cut(s) 67, 1259
SsiI CCGC 9 cut(s) 410, 564, 648, 892, 948, 1028, 1443, 1766, 2322
SspDI GGCGCC 1 cut(s) 913
SspI AATATT 1 cut(s) 2403
SspMI CTAG 4 cut(s) 132, 336, 1670, 2129
StyD4I CCNGG 4 cut(s) 461, 499, 555, 1003
TaaI ACNGT 7 cut(s) 170, 292, 445, 864, 1168, 1374, 2215
TaiI ACGT 3 cut(s) 42, 875, 1155
TaqI TCGA 6 cut(s) 629, 742, 1243, 1525, 2053, 2398
TatI WGTACW 4 cut(s) 636, 1369, 1433, 1544
TauI GCSGC 1 cut(s) 951
TfiI GAWTC 9 cut(s) 58, 176, 219, 458, 626, 631, 1139, 2046, 2078
Tru1I TTAA 8 cut(s) 23, 237, 879, 939, 1187, 1866, 2108, 2419
Tru9I TTAA 8 cut(s) 23, 237, 879, 939, 1187, 1866, 2108, 2419
TscAI CASTG 7 cut(s) 297, 937, 1037, 1187, 1205, 2218, 2277
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 5 cut(s) 92, 386, 960, 963, 1194
Tsp45I GTSAC 1 cut(s) 445
TspGWI ACGGA 1 cut(s) 2274
TspRI CASTG 7 cut(s) 297, 937, 1037, 1187, 1205, 2218, 2277
Van91I CCANNNNNTGG 1 cut(s) 379
Vha464I CTTAAG 1 cut(s) 1865
VpaK11BI GGWCC 2 cut(s) 1336, 1385
VspI ATTAAT 1 cut(s) 879
XagI CCTNNNNNAGG 1 cut(s) 2132
XapI RAATTY 7 cut(s) 718, 745, 801, 1036, 1808, 1955, 1980
XceI RCATGY 4 cut(s) 67, 1259, 1918, 2008
XhoI CTCGAG 1 cut(s) 2052
XmiI GTMKAC 1 cut(s) 1319
XmnI GAANNNNTTC 1 cut(s) 630
XspI CTAG 4 cut(s) 132, 336, 1670, 2129
ZrmI AGTACT 1 cut(s) 1371
Zsp2I ATGCAT 1 cut(s) 2254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.