RLG00000021253

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000412
Physical Location & Seq
Reverse (-)
73406681 .. 73408853
2173 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021253

Sequence Viewer

Length: 882 bp
ATGATGATTTGTATCTTGAGACCTTGGAGGGGGACAAGGCAATCTCGACTGCATCGGGGTCTTGTCGTGTGTATGGCCCCAGAAGGAGAGGAGAAACTTACTCTCATCTCGCTGCTGCAGCACTCTCGATTTTGCGACATACTATTGCTGTGCTCTTATGACAACCTATTTTGTTCTGCTAGAGATACACTAACACATGACAATCCAGTTATCGATGATGGATCAAACGCTCTTGTTTCAGCAGGAGGAGAGTTTGAACTGGGTTTCTTCCCTACTACTACAGGGAAAACATATGTGGGAATATGGTATCATAAGTTCACTCCAAGAAAAGTTGTATGGGTTGCCAACAGAGATGACCCTCTACCTGCCAATTCCATTGGATTTCTTGCAATCGAAGAAGGTAATCTCCGCCTGTTGGACAATGTTACTCGAAAGTCTTACTGGTCCACAGACATTGGAACATCTTCGTCTTCTAACATGATTGTGAAGATAATGGAGTCTGGAAACCTGGTGTTAAGGGATACTGGTGAGTTGACAGCGGATCCTTTGTGGCAGAGCTTTCAAAATCCAACTGATACATTCATTCCTGGGATGCTAATGGATAGTAACTTGGGGTTGACTTCATGGAGCGACGAAGATGACCCAAGAACTGGGAACTTCACCTTCAAACTAGATCAACAAAGTCAGTTTATTATCTTAAAAAAGTCAATGCCTTACTGGAAAACGGGAGGACCAGGTGAGGTTTTCAGGTCAGTTGAAATGCCTCGGGTAGTAACTTACTTATCAAATTTCAGCAACAGCACTGTTACAAGTATAGGGGTTGGTAAGTTCAACAAGAGCACTGTTACTAGTCCTCCCCAATTTGATTTTTTTAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

294

Amino Acids

32.83

Weight (kDa)

5.62

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 111 - 203 1.8e-23 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 373
AccB7I CCANNNNNTGG 1 cut(s) 650
AciI CCGC 2 cut(s) 409, 539
AclWI GGATC 3 cut(s) 229, 536, 549
AcsI RAATTY 1 cut(s) 787
AfiI CCNNNNNNNGG 3 cut(s) 29, 415, 650
AgsI TTSAA 5 cut(s) 257, 563, 667, 758, 832
AhlI ACTAGT 1 cut(s) 848
AjnI CCWGG 3 cut(s) 507, 586, 733
AluBI AGCT 1 cut(s) 558
AluI AGCT 1 cut(s) 558
Alw21I GWGCWC 2 cut(s) 155, 842
Alw26I GTCTC 1 cut(s) 13
AlwI GGATC 3 cut(s) 229, 536, 549
Ama87I CYCGRG 1 cut(s) 765
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 112, 115, 118
ApoI RAATTY 1 cut(s) 787
Asp700I GAANNNNTTC 1 cut(s) 463
AspS9I GGNCC 3 cut(s) 76, 444, 731
AsuHPI GGTGA 3 cut(s) 539, 652, 749
AvaI CYCGRG 1 cut(s) 765
AvaII GGWCC 2 cut(s) 444, 731
BamHI GGATCC 1 cut(s) 541
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 2 cut(s) 155, 842
BbvI GCAGC 3 cut(s) 99, 102, 130
BccI CCATC 1 cut(s) 212
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 3 cut(s) 509, 588, 735
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 13
BcuI ACTAGT 1 cut(s) 848
BfaI CTAG 3 cut(s) 180, 671, 849
BfmI CTRYAG 2 cut(s) 116, 279
BfuAI ACCTGC 1 cut(s) 373
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 3 cut(s) 113, 116, 119
BlsI GCNGC 3 cut(s) 114, 117, 120
Bme1390I CCNGG 3 cut(s) 509, 588, 735
Bme18I GGWCC 2 cut(s) 444, 731
BmeT110I CYCGRG 1 cut(s) 765
BmgT120I GGNCC 3 cut(s) 76, 444, 731
BmiI GGNNCC 2 cut(s) 78, 543
BmrFI CCNGG 3 cut(s) 509, 588, 735
BmrI ACTGGG 2 cut(s) 269, 660
BmsI GCATC 2 cut(s) 61, 582
BmuI ACTGGG 2 cut(s) 269, 660
BpiI GAAGAC 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 11
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 3 cut(s) 23, 587, 764
BsaXI ACNNNNNCTCC 2 cut(s) 838, 868
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 415, 650
Bse1I ACTGG 6 cut(s) 206, 264, 446, 529, 655, 722
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 3 cut(s) 509, 588, 735
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 23, 587, 764
BseGI GGATG 1 cut(s) 597
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 3 cut(s) 29, 415, 650
BseNI ACTGG 6 cut(s) 206, 264, 446, 529, 655, 722
BseRI GAGGAG 2 cut(s) 104, 261
BseXI GCAGC 3 cut(s) 99, 102, 130
BshFI GGCC 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 213
BsiHKAI GWGCWC 2 cut(s) 155, 842
BsiHKCI CYCGRG 1 cut(s) 765
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 3 cut(s) 29, 415, 650
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 1 cut(s) 77
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 765
Bsp1286I GDGCHC 2 cut(s) 155, 842
Bsp143I GATC 3 cut(s) 221, 541, 673
BspACI CCGC 2 cut(s) 409, 539
BspANI GGCC 1 cut(s) 77
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 2 cut(s) 78, 543
BspMAI CTGCAG 1 cut(s) 120
BspMI ACCTGC 1 cut(s) 373
BspPI GGATC 3 cut(s) 229, 536, 549
BspTNI GGTCTC 1 cut(s) 13
BsrI ACTGG 6 cut(s) 206, 264, 446, 529, 655, 722
BssECI CCNNGG 3 cut(s) 23, 587, 764
BssMI GATC 3 cut(s) 221, 541, 673
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 3 cut(s) 509, 588, 735
Bst4CI ACNGT 2 cut(s) 805, 844
BstF5I GGATG 1 cut(s) 597
BstKTI GATC 3 cut(s) 224, 544, 676
BstMAI GTCTC 1 cut(s) 13
BstMBI GATC 3 cut(s) 221, 541, 673
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 3 cut(s) 509, 588, 735
BstSCI CCNGG 3 cut(s) 507, 586, 733
BstSFI CTRYAG 2 cut(s) 116, 279
BstV1I GCAGC 3 cut(s) 99, 102, 130
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
Bsu15I ATCGAT 1 cut(s) 213
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 77
BsuTUI ATCGAT 1 cut(s) 213
BtsCI GGATG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 801, 840
BveI ACCTGC 1 cut(s) 373
Cfr13I GGNCC 3 cut(s) 76, 444, 731
ClaI ATCGAT 1 cut(s) 213
CsiI ACCWGGT 2 cut(s) 507, 733
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 3 cut(s) 197, 478, 624
CviJI RGCY 2 cut(s) 77, 558
CviKI_1 RGCY 2 cut(s) 77, 558
DpnI GATC 3 cut(s) 223, 543, 675
DpnII GATC 3 cut(s) 221, 541, 673
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 13
Eco47I GGWCC 2 cut(s) 444, 731
Eco88I CYCGRG 1 cut(s) 765
EcoRII CCWGG 3 cut(s) 507, 586, 733
EcoT14I CCWWGG 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 3 cut(s) 200, 481, 627
FaqI GGGAC 1 cut(s) 46
FatI CATG 3 cut(s) 196, 477, 623
FauNDI CATATG 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 113, 116, 119
FokI GGATG 1 cut(s) 604
Fsp4HI GCNGC 3 cut(s) 113, 116, 119
FspBI CTAG 3 cut(s) 180, 671, 849
GluI GCNGC 3 cut(s) 113, 116, 119
HaeIII GGCC 1 cut(s) 77
Hin1II CATG 3 cut(s) 200, 481, 627
HincII GTYRAC 2 cut(s) 534, 618
HindII GTYRAC 2 cut(s) 534, 618
HinfI GANTC 1 cut(s) 497
HphI GGTGA 3 cut(s) 539, 652, 749
Hpy166II GTNNAC 4 cut(s) 318, 447, 534, 618
Hpy188III TCNNGA 5 cut(s) 16, 45, 126, 501, 879
Hpy8I GTNNAC 4 cut(s) 318, 447, 534, 618
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 3 cut(s) 77, 392, 673
HpyCH4III ACNGT 2 cut(s) 805, 844
HpyCH4V TGCA 3 cut(s) 52, 118, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
Hsp92II CATG 3 cut(s) 200, 481, 627
Kzo9I GATC 3 cut(s) 221, 541, 673
LmnI GCTCC 1 cut(s) 627
Lsp1109I GCAGC 3 cut(s) 99, 102, 130
LweI GCATC 2 cut(s) 61, 582
MabI ACCWGGT 2 cut(s) 507, 733
MaeI CTAG 3 cut(s) 180, 671, 849
MaeIII GTNAC 5 cut(s) 424, 605, 772, 805, 844
MalI GATC 3 cut(s) 223, 543, 675
MboI GATC 3 cut(s) 221, 541, 673
MboII GAAGA 6 cut(s) 259, 407, 456, 462, 499, 647
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 155, 842
MluCI AATT 3 cut(s) 370, 787, 860
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 2 cut(s) 396, 593
MnlI CCTC 8 cut(s) 21, 82, 239, 369, 722, 733, 774, 864
MroXI GAANNNNTTC 1 cut(s) 463
MseI TTAA 2 cut(s) 515, 698
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 539
MspR9I CCNGG 3 cut(s) 509, 588, 735
MvaI CCWGG 3 cut(s) 509, 588, 735
MwoI GCNNNNNNNGC 1 cut(s) 118
NdeI CATATG 1 cut(s) 292
NdeII GATC 3 cut(s) 221, 541, 673
NlaIII CATG 3 cut(s) 200, 481, 627
NlaIV GGNNCC 2 cut(s) 78, 543
PdmI GAANNNNTTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 3 cut(s) 114, 117, 120
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
Psp6I CCWGG 3 cut(s) 507, 586, 733
PspGI CCWGG 3 cut(s) 507, 586, 733
PspN4I GGNNCC 2 cut(s) 78, 543
PspPI GGNCC 3 cut(s) 76, 444, 731
PsrI GAACNNNNNNTAC 2 cut(s) 299, 331
PstI CTGCAG 1 cut(s) 120
PsuI RGATCY 1 cut(s) 541
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 515, 698
SatI GCNGC 3 cut(s) 113, 116, 119
Sau3AI GATC 3 cut(s) 221, 541, 673
Sau96I GGNCC 3 cut(s) 76, 444, 731
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 3 cut(s) 509, 588, 735
SduI GDGCHC 2 cut(s) 155, 842
SexAI ACCWGGT 2 cut(s) 507, 733
SfaNI GCATC 2 cut(s) 61, 582
SfcI CTRYAG 2 cut(s) 116, 279
SinI GGWCC 2 cut(s) 444, 731
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
SpeI ACTAGT 1 cut(s) 848
Sse9I AATT 3 cut(s) 370, 787, 860
SsiI CCGC 2 cut(s) 409, 539
SspMI CTAG 3 cut(s) 180, 671, 849
StyD4I CCNGG 3 cut(s) 507, 586, 733
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 2 cut(s) 805, 844
TaqI TCGA 5 cut(s) 46, 127, 213, 393, 430
TasI AATT 3 cut(s) 370, 787, 860
Tru1I TTAA 2 cut(s) 515, 698
Tru9I TTAA 2 cut(s) 515, 698
TscAI CASTG 2 cut(s) 808, 847
TseI GCWGC 3 cut(s) 112, 115, 118
TspDTI ATGAA 2 cut(s) 571, 612
TspRI CASTG 2 cut(s) 808, 847
Van91I CCANNNNNTGG 1 cut(s) 650
VpaK11BI GGWCC 2 cut(s) 444, 731
XapI RAATTY 1 cut(s) 787
XmnI GAANNNNTTC 1 cut(s) 463
XspI CTAG 3 cut(s) 180, 671, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.