RchiOBHm_Chr2g0161081

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000412
Physical Location & Seq
Reverse (-)
76942955 .. 76950184
7230 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52945

Sequence Viewer

Length: 3438 bp
ATGCAAGCTGTAGAAGCTAGATTTATGATGACTTTGTATAACAACGTGGAGTCTTACATCTTTTTCTTGTATGTGGTACTATTGCTGTGCTCTTATGACAACCTATTTTGTTCTGCTAGAGATACACCAACACATGACAATCCAGTTATCGATGATGGATCAGATGCTCTTGTTTCAGCAGGAGGAGAGTTTGAACTGGGTTTCTTCCCTACTACTACATGGAAAACATATGTGGGAATATGGTATCATAAGGTCACTCCAAGAAAAGTTGTATGGGTTGCCAACAGAGACGACCCTCTACCTGCCAATTCCATTGGAGTTCTTGCAATCCAAGAAGGTAATCTCCGCTTGTTGGACAATGTTACTCGAAAGTCTCATTGGTCCACAGACATTGGAACATCTTCGTCTTCTAACATGATTGTGAAGATAATGGAGTCTGGAAACCTGGTGTTAAGGGATACTGGTGAGTTGACAGTGGATCCTTTGTGGCAGAGCTTTCAAAATCCAACTGATACATTCATTCCTGGGATGCTAATGGATAGTAACTTGGGGTTGACTTCATGGAGCGACGAAGATGACCCAAGAACTGGGAACTTCACCTTCAAACTAGATCAACAAAGTCTGTTTATTATCTTAAAAAATTCAATGCCTTACTGGAAAACGGGAGGACCAGGTGAGGTTTTCAGGTCAGTTGAAATGCCTTGGGTAGTAACTTACTTATTATCAAATTTCAGCAACAGCACTGTTACAAGTATAGGGATTGGTAAGTTCAACAAGAGCACTGTTACTAGTCCTCCCCGATTTGATTGCACTCATGCAAGATTGGTGATATCCCCTAGTGGAGAGCTGAAGATTTCAACTTGGATTGAAGACTTGCAGTGGGTTCCGGTTTGGTCAGAGCCAAAAGATCAATGCAGTGTGGATAATGCTTGTGGGAACTTTGGCAGCTGTAATACTAATAACAACCCGGTGGTGTGCAAATGTTTGCCAGGTTTTAAGCCCCAGTCTCTAGATAAATGGACTTCTGGAGACTTCTCAGATGGATACACAAAAGACTTGTCAATATGTGGGCATGATGACACCTTTTTGAGTTTGAAGATGATGAAAGTGGGTAACCCAGACACAGAGATTGTTGTTTACGATGAAATAGAATGCAAGAACAAGTGCCTCAACAGCTGCCAGTGCCGGGCATACTCATATGCTGCACCTGAAAGCATAGCTCAAAGGGATCTTCGTAAAACTTCCTTGTGCAGGGTATGGCTTAAGGACTTAAATAGTCTTGTAGAAGAGGATGACAATAGTCATGACATTGGTGCTCGAGTTGCCTTCTCTGATATAGAATCAACTATAAGAGACTGCAGCCCTTGTGGCACAACTACAATTCCTTATCCTCTGAGCACTAGATCAGATTGTGGCGACGCCATGTACTTAAGTTTCGCGTGCAATGCCTCCATTAATGAATTTAGCTTCCTGGGACCAGATGGCACCTCCTTTAGAGATATAAATATCAGCCGAAGTACACAACGATTCATTATCAAATCCAAAAATATGAATTATTGTGATTCTAGAAACCGAGTGCTGAACATTTCCTTCCTGTTTAAGGTAATCAGTTGGTGTAGCTCTGACCTGATGAGGAATTTTATTTCCAGCATAAATTCATCCAGCGGTCCTTCTGTAATAGAGATGGGTTGGGAGCCACCATCGGAAGCAAGCTGCACTACCTTAGAAGACTACAGGGATTGGCCTATTCAACCTGCAATCCATGAAAGACGAGTCAAAGAGCTCATAGACACGAGTGACTTCAAAGAAGAAGATGACAAAGGCATAGATGTACCTTTTTTTGATTTGCAAAGCATACTAGACTCTACAGATAATTTCTCAATTGGTAAATTAGAAGAACTTGGGATCTTTCCTGGAGGTCAAAAAATTGCTGTAAAGAGGCTATCAAGAGCTTCAGGACAAGGCTTACAGGAGTTTAAGAATGAGGTGGTGCTAATTGCCAAAATTCAACATCGTAATCTTGTAAGACTCAGAGGATATTGCATGAAAGGAGAAGAAAAGATTTTACTCTACGACTACATGCCTAACAAAAGCTTAGATTCCTTTATATTTGATTCCAGGTTGAGGATCATTCATAGAGATTTGAAAACCAGCAACGTTCTTTTCGATGAGGAGATGAACCCCAAGATGTCTGACTTCGGCTTGGCCAGGATTGTTGGAGGCAAAGAAACCGAGGCAAACACAAATACAGTAGTAGGAACCTATGGTTACATGTCGCCGGAATATGCATTAGATGGAAAAATCTCTGTCAAATCAGATGTCCTTAGTTTCGGTGTGGTTCTTCTAGAGATAATCAGCGGAAAAAAGAATGCAGGATTTTATCAGTCAAAACAAACTTTCAGTCTCCTTAGTTATACATGGGGACTCTGGACAGAAAACAAGGTGTTGGATTTAATGGACAACAATTTGGAAGAAAGTTGCAACAGAAATACTACCTCCTTTAGAGTTATAAATATCAGCCGAAGTACAGGACGATTCATTATCAAATCCAAAAATATGGATTATTGTGATTCTAGAAGCAGAGTGCTGAATATTTCCCTCCCGTTTAAGGTAATCAGTTGGTGCAGTTCTGACCTGATGAAGAATATTAATTGCAGCGTAAATTCATCCAGCGGTCCTTCTGTAATGGAGATTGGTTGGGAGCCACCCTCGGAACCAACCTGCACTACATTAAAAGACTGCAGGGATTGGCCAAATTTAACTTGCAATCTAGTAAGAGGTGGAAAGAAAAAATGCCTTTGCAATACAAATTTTCAATGGAACGGCTCTAACTTAAATTGTACTCAAGAAGGAAATCTACAAAAGCTATCAAGTAAGAGTTCAAGGAAAAAATTGTTATCGTCTCTGGTTGTTGTGGCAGTCATAAGTGGGATTTTTCTGGCATGCAGCATTGGATTTTGTATGTGGAGAAGAAATATGACTAAGAGACGAGTGAGTTGTTTGCAGCTTGATAGTGAAAGACAAGTCAAAGAGCTCATAGACACGAGTGACTTCAAGGAAGTAGATGATAAAGGTGTAGATGTACCTTTTTTTGATTTGCAAAGCATACTAGATGCTACAGATAATTTTTCAATTGCAAACAAGCTTGGACAAGGGAGGTATGGCCCTGTTTACAAGGGGATCTTTCCTGGAGGTCAAGAAATTGCTGTAAAGAGGCTGTCAAGAGTTTCAGGACAAGTCTTACAGGAATTTAAGAATGAGGTGGTGCCAATCGCCATACTTCAACATCGTAATCTTGTTAGACTCGGAGGATATTGCATGAGAGGAGAAGAAAAGATTTTACTTTATGAGTACATGCACAACAAAAGCTTAGATTCCTTTATATTTGGTTGGCTTCTACTCCTCAGTTTTACTTTCTTTCTTTTTTATCCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1145

Amino Acids

128.99

Weight (kDa)

5.69

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 90 - 182 3.2e-22 D-mannose binding lectin
S_locus_glycop PF00954 263 - 335 5.3e-17 S-locus glycoprotein domain
PAN_2 PF08276 356 - 426 4.4e-11 PAN-like domain
PK_Tyr_Ser-Thr PF07714 641 - 802 1.5e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 709 - 790 6e-20 Protein kinase domain
Pkinase PF00069 1044 - 1128 3.4e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1045 - 1128 4.7e-11 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2510
Acc36I ACCTGC 3 cut(s) 310, 1762, 2729
AccB1I GGYRCC 2 cut(s) 1484, 3265
AccB7I CCANNNNNTGG 1 cut(s) 587
AccII CGCG 1 cut(s) 1439
AciI CCGC 4 cut(s) 346, 1665, 2358, 2673
AclI AACGTT 1 cut(s) 2157
AclWI GGATC 7 cut(s) 166, 473, 486, 1236, 1913, 2135, 3188
AcoI YGGCCR 2 cut(s) 2205, 2750
AcuI CTGAAG 2 cut(s) 869, 1938
AcyI GRCGYC 1 cut(s) 1419
AfaI GTAC 8 cut(s) 78, 1427, 1519, 1833, 2527, 2842, 3084, 3353
AfiI CCNNNNNNNGG 7 cut(s) 352, 587, 1186, 1251, 1600, 2124, 2608
AflII CTTAAG 2 cut(s) 1262, 1429
AflIII ACRYGT 1 cut(s) 2271
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 9 cut(s) 444, 523, 670, 988, 1470, 1912, 2117, 2207, 3187
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
Alw21I GWGCWC 6 cut(s) 92, 782, 1318, 1400, 1785, 3036
Alw26I GTCTC 8 cut(s) 282, 378, 1011, 1023, 1347, 2408, 2907, 2980
AlwI GGATC 7 cut(s) 166, 473, 486, 1236, 1913, 2135, 3188
Ama87I CYCGRG 1 cut(s) 1317
AoxI GGCC 4 cut(s) 1742, 2205, 2750, 3163
ApeKI GCWGC 8 cut(s) 945, 1176, 1202, 1359, 1713, 2655, 2946, 3004
ArsI GACNNNNNNTTYG 6 cut(s) 1043, 1075, 2304, 2336, 2386, 2418
AseI ATTAAT 2 cut(s) 1455, 2649
Asp700I GAANNNNTTC 1 cut(s) 400
AspS9I GGNCC 6 cut(s) 381, 668, 1475, 1667, 2675, 3164
AsuC2I CCSGG 2 cut(s) 968, 1187
AsuHPI GGTGA 4 cut(s) 476, 589, 686, 838
AvaI CYCGRG 1 cut(s) 1317
AvaII GGWCC 5 cut(s) 381, 668, 1475, 1667, 2675
BalI TGGCCA 2 cut(s) 2207, 2752
BamHI GGATCC 1 cut(s) 478
BanI GGYRCC 2 cut(s) 1484, 3265
BanII GRGCYC 2 cut(s) 1785, 3036
BarI GAAGNNNNNNTAC 2 cut(s) 2841, 2873
BauI CACGAG 2 cut(s) 1792, 3043
BbsI GAAGAC 3 cut(s) 399, 876, 1734
Bbv12I GWGCWC 6 cut(s) 92, 782, 1318, 1400, 1785, 3036
BbvI GCAGC 8 cut(s) 957, 1163, 1189, 1371, 1700, 2667, 2958, 3016
BccI CCATC 6 cut(s) 149, 1034, 1475, 1678, 1708, 2288
BceAI ACGGC 1 cut(s) 2839
BcgI CGANNNNNNTGC 2 cut(s) 789, 823
BciT130I CCWGG 9 cut(s) 446, 525, 672, 990, 1472, 1914, 2119, 2209, 3189
BciVI GTATCC 2 cut(s) 451, 1037
BcnI CCSGG 2 cut(s) 968, 1187
BcoDI GTCTC 8 cut(s) 282, 378, 1011, 1023, 1347, 2408, 2907, 2980
BcuI ACTAGT 1 cut(s) 788
BfmI CTRYAG 6 cut(s) 9, 1357, 1732, 1866, 2740, 3117
BfrI CTTAAG 2 cut(s) 1262, 1429
BfuAI ACCTGC 3 cut(s) 310, 1762, 2729
BfuI GTATCC 2 cut(s) 451, 1037
BglI GCCNNNNNGGC 1 cut(s) 1368
BisI GCNGC 8 cut(s) 946, 1177, 1203, 1360, 1714, 2656, 2947, 3005
BlsI GCNGC 8 cut(s) 947, 1178, 1204, 1361, 1715, 2657, 2948, 3006
Bme18I GGWCC 5 cut(s) 381, 668, 1475, 1667, 2675
BmeT110I CYCGRG 1 cut(s) 1317
BmgT120I GGNCC 6 cut(s) 381, 668, 1475, 1667, 2675, 3164
BmiI GGNNCC 9 cut(s) 480, 885, 1476, 1486, 1695, 2260, 2703, 2715, 3267
BmrI ACTGGG 3 cut(s) 206, 597, 997
BmsI GCATC 3 cut(s) 154, 519, 3103
BmuI ACTGGG 3 cut(s) 206, 597, 997
BoxI GACNNNNGTC 1 cut(s) 1299
BpiI GAAGAC 3 cut(s) 399, 876, 1734
BplI GAGNNNNNCTC 2 cut(s) 2693, 2725
BpmI CTGGAG 3 cut(s) 1047, 1935, 3210
BpuEI CTTGAG 1 cut(s) 2829
BpuMI CCSGG 2 cut(s) 968, 1187
Bsa29I ATCGAT 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 1419
BsaJI CCNNGG 5 cut(s) 524, 701, 1471, 2232, 2709
BsaWI WCCGGW 1 cut(s) 886
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 7 cut(s) 352, 587, 1186, 1251, 1600, 2124, 2608
Bse1I ACTGG 7 cut(s) 143, 201, 466, 592, 659, 1003, 1180
Bse3DI GCAATG 1 cut(s) 1450
BseBI CCWGG 9 cut(s) 446, 525, 672, 990, 1472, 1914, 2119, 2209, 3189
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 5 cut(s) 524, 701, 1471, 2232, 2709
BseGI GGATG 4 cut(s) 534, 1297, 1658, 2666
BseLI CCNNNNNNNGG 7 cut(s) 352, 587, 1186, 1251, 1600, 2124, 2608
BseMI GCAATG 1 cut(s) 1450
BseMII CTCAG 4 cut(s) 1050, 1385, 2044, 3418
BseNI ACTGG 7 cut(s) 143, 201, 466, 592, 659, 1003, 1180
BseRI GAGGAG 4 cut(s) 198, 2186, 3339, 3392
BseXI GCAGC 8 cut(s) 957, 1163, 1189, 1371, 1700, 2667, 2958, 3016
BsgI GTGCAG 5 cut(s) 1188, 1270, 1699, 2644, 2707
Bsh1236I CGCG 1 cut(s) 1439
BshFI GGCC 4 cut(s) 1744, 2207, 2752, 3165
BshNI GGYRCC 2 cut(s) 1484, 3265
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 6 cut(s) 92, 782, 1318, 1400, 1785, 3036
BsiHKCI CYCGRG 1 cut(s) 1317
BsiSI CCGG 4 cut(s) 887, 968, 1186, 2279
BslFI GGGAC 2 cut(s) 1488, 2436
BslI CCNNNNNNNGG 7 cut(s) 352, 587, 1186, 1251, 1600, 2124, 2608
BsmAI GTCTC 8 cut(s) 282, 378, 1011, 1023, 1347, 2408, 2907, 2980
BsmBI CGTCTC 3 cut(s) 282, 2907, 2980
BsmFI GGGAC 2 cut(s) 1488, 2436
BsmI GAATGC 2 cut(s) 1157, 2374
BsnI GGCC 4 cut(s) 1744, 2207, 2752, 3165
BsoBI CYCGRG 1 cut(s) 1317
Bsp1286I GDGCHC 6 cut(s) 92, 782, 1318, 1400, 1785, 3036
Bsp143I GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
BspACI CCGC 4 cut(s) 346, 1665, 2358, 2673
BspANI GGCC 4 cut(s) 1744, 2207, 2752, 3165
BspCNI CTCAG 4 cut(s) 1049, 1386, 2043, 3417
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 1 cut(s) 1439
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 9 cut(s) 480, 885, 1476, 1486, 1695, 2260, 2703, 2715, 3267
BspMAI CTGCAG 2 cut(s) 1361, 2744
BspMI ACCTGC 3 cut(s) 310, 1762, 2729
BspPI GGATC 7 cut(s) 166, 473, 486, 1236, 1913, 2135, 3188
BspT107I GGYRCC 2 cut(s) 1484, 3265
BspTI CTTAAG 2 cut(s) 1262, 1429
BsrDI GCAATG 1 cut(s) 1450
BsrI ACTGG 7 cut(s) 143, 201, 466, 592, 659, 1003, 1180
BssECI CCNNGG 5 cut(s) 524, 701, 1471, 2232, 2709
BssMI GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
BssNI GRCGYC 1 cut(s) 1419
BssSI CACGAG 2 cut(s) 1792, 3043
BssT1I CCWWGG 1 cut(s) 701
Bst2BI CACGAG 2 cut(s) 1792, 3043
Bst2UI CCWGG 9 cut(s) 446, 525, 672, 990, 1472, 1914, 2119, 2209, 3189
Bst4CI ACNGT 4 cut(s) 475, 745, 784, 2251
Bst6I CTCTTC 1 cut(s) 1281
BstACI GRCGYC 1 cut(s) 1419
BstAFI CTTAAG 2 cut(s) 1262, 1429
BstC8I GCNNGC 4 cut(s) 6, 1441, 1711, 2944
BstEII GGTNACC 1 cut(s) 1112
BstENI CCTNNNNNAGG 2 cut(s) 1249, 1598
BstF5I GGATG 4 cut(s) 534, 1297, 1658, 2666
BstFNI CGCG 1 cut(s) 1439
BstKTI GATC 9 cut(s) 161, 481, 613, 910, 1231, 1406, 1908, 2130, 3183
BstMAI GTCTC 8 cut(s) 282, 378, 1011, 1023, 1347, 2408, 2907, 2980
BstMBI GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
BstMWI GCNNNNNNNGC 6 cut(s) 14, 1173, 1182, 1322, 1368, 1445
BstNI CCWGG 9 cut(s) 446, 525, 672, 990, 1472, 1914, 2119, 2209, 3189
BstNSI RCATGY 4 cut(s) 2083, 2275, 2946, 3358
BstPAI GACNNNNGTC 1 cut(s) 1299
BstPI GGTNACC 1 cut(s) 1112
BstSFI CTRYAG 6 cut(s) 9, 1357, 1732, 1866, 2740, 3117
BstUI CGCG 1 cut(s) 1439
BstV1I GCAGC 8 cut(s) 957, 1163, 1189, 1371, 1700, 2667, 2958, 3016
BstV2I GAAGAC 3 cut(s) 399, 876, 1734
BstX2I RGATCY 4 cut(s) 478, 1228, 1905, 3180
BstXI CCANNNNNNTGG 1 cut(s) 2557
BstYI RGATCY 4 cut(s) 478, 1228, 1905, 3180
Bsu15I ATCGAT 1 cut(s) 150
BsuI GTATCC 2 cut(s) 451, 1037
BsuRI GGCC 4 cut(s) 1744, 2207, 2752, 3165
BsuTUI ATCGAT 1 cut(s) 150
BtsCI GGATG 4 cut(s) 534, 1297, 1658, 2666
BtsI GCAGTG 2 cut(s) 884, 922
BtsIMutI CAGTG 6 cut(s) 480, 741, 780, 884, 922, 1187
BveI ACCTGC 3 cut(s) 310, 1762, 2729
Cac8I GCNNGC 4 cut(s) 6, 1441, 1711, 2944
CciI TCATGA 1 cut(s) 1303
Cfr13I GGNCC 6 cut(s) 381, 668, 1475, 1667, 2675, 3164
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 1 cut(s) 1427
CsiI ACCWGGT 2 cut(s) 444, 670
Csp6I GTAC 8 cut(s) 77, 1426, 1518, 1832, 2526, 2841, 3083, 3352
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviQI GTAC 8 cut(s) 77, 1426, 1518, 1832, 2526, 2841, 3083, 3352
DpnI GATC 9 cut(s) 160, 480, 612, 909, 1230, 1405, 1907, 2129, 3182
DpnII GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
EaeI YGGCCR 2 cut(s) 2205, 2750
Eam1104I CTCTTC 1 cut(s) 1281
EarI CTCTTC 1 cut(s) 1281
Ecl136II GAGCTC 2 cut(s) 1783, 3034
Eco130I CCWWGG 1 cut(s) 701
Eco24I GRGCYC 2 cut(s) 1785, 3036
Eco32I GATATC 1 cut(s) 831
Eco47I GGWCC 5 cut(s) 381, 668, 1475, 1667, 2675
Eco53kI GAGCTC 2 cut(s) 1783, 3034
Eco57I CTGAAG 2 cut(s) 869, 1938
Eco88I CYCGRG 1 cut(s) 1317
Eco91I GGTNACC 1 cut(s) 1112
EcoICRI GAGCTC 2 cut(s) 1783, 3034
EcoNI CCTNNNNNAGG 2 cut(s) 1249, 1598
EcoO65I GGTNACC 1 cut(s) 1112
EcoRII CCWGG 9 cut(s) 444, 523, 670, 988, 1470, 1912, 2117, 2207, 3187
EcoRV GATATC 1 cut(s) 831
EcoT14I CCWWGG 1 cut(s) 701
EcoT22I ATGCAT 1 cut(s) 2290
EcoT38I GRGCYC 2 cut(s) 1785, 3036
ErhI CCWWGG 1 cut(s) 701
Esp3I CGTCTC 3 cut(s) 282, 2907, 2980
FalI AAGNNNNNCTT 2 cut(s) 3167, 3199
FaqI GGGAC 2 cut(s) 1488, 2436
FauNDI CATATG 2 cut(s) 229, 1198
Fnu4HI GCNGC 8 cut(s) 946, 1177, 1203, 1360, 1714, 2656, 2947, 3005
FokI GGATG 4 cut(s) 541, 1304, 1645, 2653
FriOI GRGCYC 2 cut(s) 1785, 3036
Fsp4HI GCNGC 8 cut(s) 946, 1177, 1203, 1360, 1714, 2656, 2947, 3005
GluI GCNGC 8 cut(s) 946, 1177, 1203, 1360, 1714, 2656, 2947, 3005
GsuI CTGGAG 3 cut(s) 1047, 1935, 3210
HaeIII GGCC 4 cut(s) 1744, 2207, 2752, 3165
HapII CCGG 4 cut(s) 887, 968, 1186, 2279
HgaI GACGC 1 cut(s) 1427
Hin1I GRCGYC 1 cut(s) 1419
HincII GTYRAC 2 cut(s) 471, 555
HindII GTYRAC 2 cut(s) 471, 555
HindIII AAGCTT 3 cut(s) 2092, 3143, 3367
HpaII CCGG 4 cut(s) 887, 968, 1186, 2279
HphI GGTGA 4 cut(s) 476, 589, 686, 838
Hpy166II GTNNAC 6 cut(s) 384, 471, 555, 1138, 1520, 3172
Hpy8I GTNNAC 6 cut(s) 384, 471, 555, 1138, 1520, 3172
Hpy99I CGWCG 2 cut(s) 572, 1421
HpyAV CCTTC 7 cut(s) 329, 610, 1336, 1600, 1680, 2688, 2843
HpyCH4III ACNGT 4 cut(s) 475, 745, 784, 2251
HpyCH4IV ACGT 2 cut(s) 45, 2157
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 1173, 1182, 1322, 1368, 1445
HpySE526I ACGT 2 cut(s) 45, 2157
Hsp92I GRCGYC 1 cut(s) 1419
Kzo9I GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
LmnI GCTCC 3 cut(s) 564, 1693, 2701
Lsp1109I GCAGC 8 cut(s) 957, 1163, 1189, 1371, 1700, 2667, 2958, 3016
LweI GCATC 3 cut(s) 154, 519, 3103
MabI ACCWGGT 2 cut(s) 444, 670
MaeII ACGT 2 cut(s) 45, 2157
MalI GATC 9 cut(s) 160, 480, 612, 909, 1230, 1405, 1907, 2129, 3182
MboI GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
MfeI CAATTG 2 cut(s) 1881, 3132
MflI RGATCY 4 cut(s) 478, 1228, 1905, 3180
MhlI GDGCHC 6 cut(s) 92, 782, 1318, 1400, 1785, 3036
MlsI TGGCCA 2 cut(s) 2207, 2752
MluNI TGGCCA 2 cut(s) 2207, 2752
MlyI GAGTC 7 cut(s) 59, 443, 1782, 1856, 2022, 2418, 3297
MmeI TCCRAC 4 cut(s) 333, 530, 2197, 2427
Mox20I TGGCCA 2 cut(s) 2207, 2752
Mph1103I ATGCAT 1 cut(s) 2290
MroXI GAANNNNTTC 1 cut(s) 400
MscI TGGCCA 2 cut(s) 2207, 2752
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 2 cut(s) 2207, 2752
MspA1I CMGCKG 5 cut(s) 948, 1176, 1665, 2358, 2673
MspCI CTTAAG 2 cut(s) 1262, 1429
MspI CCGG 4 cut(s) 887, 968, 1186, 2279
MunI CAATTG 2 cut(s) 1881, 3132
Mva1269I GAATGC 2 cut(s) 1157, 2374
MvaI CCWGG 9 cut(s) 446, 525, 672, 990, 1472, 1914, 2119, 2209, 3189
MvnI CGCG 1 cut(s) 1439
MwoI GCNNNNNNNGC 6 cut(s) 14, 1173, 1182, 1322, 1368, 1445
NciI CCSGG 2 cut(s) 968, 1187
NdeI CATATG 2 cut(s) 229, 1198
NdeII GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
NlaIV GGNNCC 9 cut(s) 480, 885, 1476, 1486, 1695, 2260, 2703, 2715, 3267
NmuCI GTSAC 3 cut(s) 253, 1796, 3047
NsiI ATGCAT 1 cut(s) 2290
NspI RCATGY 4 cut(s) 2083, 2275, 2946, 3358
PaeI GCATGC 1 cut(s) 2946
PaeR7I CTCGAG 1 cut(s) 1317
PagI TCATGA 1 cut(s) 1303
PciI ACATGT 1 cut(s) 2271
PcsI WCGNNNNNNNCGW 1 cut(s) 2163
PctI GAATGC 2 cut(s) 1157, 2374
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 8 cut(s) 1340, 1527, 1562, 2099, 2114, 2535, 2570, 3374
PflMI CCANNNNNTGG 1 cut(s) 587
PfoI TCCNGGA 2 cut(s) 1912, 3187
PkrI GCNGC 8 cut(s) 947, 1178, 1204, 1361, 1715, 2657, 2948, 3006
PleI GAGTC 7 cut(s) 58, 442, 1781, 1856, 2022, 2418, 3297
PpsI GAGTC 7 cut(s) 58, 442, 1781, 1856, 2022, 2418, 3297
PscI ACATGT 1 cut(s) 2271
PshAI GACNNNNGTC 1 cut(s) 1299
PshBI ATTAAT 2 cut(s) 1455, 2649
PsiI TTATAA 1 cut(s) 2510
Psp124BI GAGCTC 2 cut(s) 1785, 3036
Psp1406I AACGTT 1 cut(s) 2157
Psp6I CCWGG 9 cut(s) 444, 523, 670, 988, 1470, 1912, 2117, 2207, 3187
PspEI GGTNACC 1 cut(s) 1112
PspGI CCWGG 9 cut(s) 444, 523, 670, 988, 1470, 1912, 2117, 2207, 3187
PspN4I GGNNCC 9 cut(s) 480, 885, 1476, 1486, 1695, 2260, 2703, 2715, 3267
PspPI GGNCC 6 cut(s) 381, 668, 1475, 1667, 2675, 3164
PspXI VCTCGAGB 1 cut(s) 1317
PstI CTGCAG 2 cut(s) 1361, 2744
PsuI RGATCY 4 cut(s) 478, 1228, 1905, 3180
PvuII CAGCTG 2 cut(s) 948, 1176
RsaI GTAC 8 cut(s) 78, 1427, 1519, 1833, 2527, 2842, 3084, 3353
RsaNI GTAC 8 cut(s) 77, 1426, 1518, 1832, 2526, 2841, 3083, 3352
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 2 cut(s) 1785, 3036
SatI GCNGC 8 cut(s) 946, 1177, 1203, 1360, 1714, 2656, 2947, 3005
Sau3AI GATC 9 cut(s) 158, 478, 610, 907, 1228, 1403, 1905, 2127, 3180
Sau96I GGNCC 6 cut(s) 381, 668, 1475, 1667, 2675, 3164
SchI GAGTC 7 cut(s) 59, 443, 1782, 1856, 2022, 2418, 3297
SduI GDGCHC 6 cut(s) 92, 782, 1318, 1400, 1785, 3036
SexAI ACCWGGT 2 cut(s) 444, 670
SfaNI GCATC 3 cut(s) 154, 519, 3103
SfcI CTRYAG 6 cut(s) 9, 1357, 1732, 1866, 2740, 3117
Sfr274I CTCGAG 1 cut(s) 1317
SinI GGWCC 5 cut(s) 381, 668, 1475, 1667, 2675
SlaI CTCGAG 1 cut(s) 1317
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 4 cut(s) 1262, 1317, 1429, 2844
SmoI CTYRAG 4 cut(s) 1262, 1317, 1429, 2844
SpeI ACTAGT 1 cut(s) 788
SphI GCATGC 1 cut(s) 2946
SsiI CCGC 4 cut(s) 346, 1665, 2358, 2673
SspI AATATT 2 cut(s) 2593, 2647
SstI GAGCTC 2 cut(s) 1785, 3036
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 4 cut(s) 475, 745, 784, 2251
TaiI ACGT 2 cut(s) 48, 2160
TaqI TCGA 4 cut(s) 150, 367, 1318, 2166
TatI WGTACW 5 cut(s) 1425, 1517, 2525, 2840, 3351
TfiI GAWTC 8 cut(s) 1340, 1527, 1562, 2099, 2114, 2535, 2570, 3374
TscAI CASTG 6 cut(s) 480, 748, 787, 884, 922, 1187
TseFI GTSAC 3 cut(s) 253, 1796, 3047
TseI GCWGC 8 cut(s) 945, 1176, 1202, 1359, 1713, 2655, 2946, 3004
Tsp45I GTSAC 3 cut(s) 253, 1796, 3047
TspRI CASTG 6 cut(s) 480, 748, 787, 884, 922, 1187
Van91I CCANNNNNTGG 1 cut(s) 587
Vha464I CTTAAG 2 cut(s) 1262, 1429
VpaK11BI GGWCC 5 cut(s) 381, 668, 1475, 1667, 2675
VspI ATTAAT 2 cut(s) 1455, 2649
XagI CCTNNNNNAGG 2 cut(s) 1249, 1598
XbaI TCTAGA 4 cut(s) 1009, 1565, 2344, 2573
XceI RCATGY 4 cut(s) 2083, 2275, 2946, 3358
XhoI CTCGAG 1 cut(s) 1317
XmnI GAANNNNTTC 1 cut(s) 400
Zsp2I ATGCAT 1 cut(s) 2290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.