Rmu_sc0000955.1_g000023

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000955.1
OG0000412
Physical Location & Seq
Reverse (-)
87292 .. 94073
6782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000955.1_g000023.1.cds

Sequence Viewer

Length: 3468 bp
atgttcactaacttctttctgtattcattcctgttatgcacttttcttcattgtttcattgctaaagatacaatagcatataacagtttgattagtgatgacaaaggtgatactattgtttcatcaggagaaaaatttgaacttgggttctttactcctaatggaagctctggtactagaagatatgttggaatttggtattacagatcgaatccacagactgttgtttggcttgcaaatagagacaaccctctttcagatactcgtggagttttcactattgcagaagatggaaatcttaaggtgctggatggcaaaagaaaaacttactggtcatcaagtcttgaaagttcatcatcaacaattaggacagcaaagctcatggacactggtaatcttgttgtgatcaatagagagcaaggaaacaactcggttcaaattgtctggcagagttttgaaaatccaaccgacacatttcttcctggcatgaaaatgagtggaaaattagtactgagttcatggaagagttacaatgatccagcaacaggaaatttcacatttcagcaagaccaagaggatgcgaaccactttgttatctggaaaagatcgagaaggtactggaagagtgaagattatggcaacttcattagttctgatgagatggcttctgcaattctctacttgctatcaaacttcacctctacagcagtccacaatgactctgtgccatatctcacctcctcattatacacttctacaaggctggttatgagtttttcaggccagattcagtatctgatgtgggatagtgagaaggtttggtctttgatatgggcagatcctagagatagatgcagtgtgtataatgcatgtggaaattttggtagctgtaacagcaaaaatggtttggtttgcaagtgtttgcctggttttaagcctagttcaccggataattggaatcatggagattattcgggtggatgcactaggaagtcaacattatgtggcagcaatgctgagagtgacacattcttgagcttaaagatgatgaaagtgggaaatccagattcccaatttaatgccaaaagtgaagtggaatgcagagtagagtgccttaacaactgtgagtgtcaagcttatttctatgaagaggtggagaacaaaaaaacgggtgggagtagtagttcaacatgttggatttggtcacaagatgtcaccaatcttcaggaggactatgacggtggccagaatctacaggttcgtgtagcagtttcagatatagaatcaacagcgagaagctgtggaagttgtggcacaaacctgatcccttatcccttaagcactggaccaaagtgtggtgatgtcacctactatagtttccactgcaatatttcaactggccagctgagctttgaggcaccaagcggcacctaccatgtcacaagcatcaacgcagacacacaaacatttgtcatccaagctaatgatgcagatgaatgtagagataaaaaatttctgaagctcatacagtcttctccatataatgtgacaaacatgtgcaatgctgatccgacccgttttagtcctgatttgtcatttaaaggaggatatgaagttgaagttgcttgggagtcacccttggaaccaccttgttcctcatctacagactgcaaggactggcctcgttcgatatgtgatgctgctctagatgggaagaataggtgtctttgccctgcaaactcaaaatgggatagcaggagtttaaattgtactcaagaagttggccacagaaagcaaactggtgagcaagggaagatgaccctagctctaatcattgcagtaacttgtataagtgtagctgttctagcaattctttcaagtacctttgtttatgcttatatatggagaagaaggcgcattaagacacaaggccgatttaaggatgacgatacggagggcattgatgtaccctcttttgatttggaaagcatactagtagctacaacatacttctccaatgcaaataaacttggacaaggaggatttggtcctgtttacaagggtaagcttccaggaggagaagaaatcgctgtaaagaggctctcgagttgttcaggccaaggcttagaggaattcaaaaatgaagttttgttaattgccaaacttcaacatcggaatctggttcgacttttgggctattgtgctgagggagatgaaaagatgttaatctatgaatacatggccaacaaaagcttagactctttcatctttggaattgctcgggggcttctttatcttcaccaagattctagattaagggttattcatagagatctgaaaaccagcaacattctactgagtgaagagatgaaccccaaaatatcagacttcggtttggcaaggatctttggaggcaatgaaacttcagcaaacaccaatagagtagtgggaacatacggctatatgtctccagagtatgcattagatgggttattctcagtaaaatctgatgtttttagctttggtgtagttgtgattgaaatcatcactgggaaaaggaacacaggattttatcagcctgaaagatctttgagtcttcttggctatgcatggcatttgtggaaagaacaaaaggcgttagatttgctagaacaaacacttggtcacagctgcaacaaggatgagtacttcaagtgtgttaatgttgggctcttatgtgtacaagaagacccaggtgatcggccaaccatgtcacaagtagttttcatgctcggaagtgaaactgcaacgattccaacccctaaacaacccgctttcgttgttaggcgatgcccttctagttctagcagggcttctaattcttcaagcaaaccagaaacgccaccaatccgatttcactgtccctccaaaacccacagcagcatatcgaagcgcaacgattcactgcatcgcaagcccacctgcctttgcgtccctccaacccactgcaacccgccgagcgataccagcccaggccaccgaactccagcagcagcagccccggcctccgacctgcagcagcccaagcctccgatcagccttcctccgcatagcccgcgcaccagccccctctgcacaccggcgaactgcagaagaaccagaggaagaaaaactgagaaacatgataacatactcgtgaggaattataattcaaggcccattggaactaagagcgttcatgaggcgaattataatgcacctaaaggaaggcgcaaggagcggtaccctaagaatgggggacaagagggacgtatgggcccatataaccgccctaaccaggaaggaaaccgcaagtttggtgtggatacacgtggtggtaatgccacacgtgggagagggggtcgtggtatccctagcagtaatggtggctcaatgggtcgtggtagtggcaccaaccctcctagggaacgcccacaacgtgcaccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1155

Amino Acids

128.26

Weight (kDa)

8.6

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3186, 3231
AarI CACCTGC 1 cut(s) 2969
Acc36I ACCTGC 2 cut(s) 2969, 3060
Acc65I GGTACC 1 cut(s) 3261
AccB1I GGYRCC 4 cut(s) 1417, 1427, 3261, 3428
AccB7I CCANNNNNTGG 1 cut(s) 1355
AccBSI CCGCTC 1 cut(s) 3259
AccII CGCG 1 cut(s) 3097
AciI CCGC 8 cut(s) 1425, 2811, 2993, 3086, 3095, 3259, 3307, 3328
AclWI GGATC 5 cut(s) 530, 833, 1318, 1562, 2411
AcoI YGGCCR 5 cut(s) 1243, 1399, 1783, 2241, 2741
AcsI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2132
AcuI CTGAAG 3 cut(s) 1208, 1538, 2409
AcvI CACGTG 2 cut(s) 3350, 3368
AdeI CACNNNGTG 2 cut(s) 3353, 3458
AfaI GTAC 9 cut(s) 175, 510, 617, 1771, 1882, 1968, 2687, 2721, 3263
AfiI CCNNNNNNNGG 7 cut(s) 545, 1355, 1939, 3272, 3316, 3369, 3442
AflII CTTAAG 2 cut(s) 299, 1336
AflIII ACRYGT 4 cut(s) 1190, 1554, 3347, 3365
AhlI ACTAGT 1 cut(s) 1993
AjnI CCWGG 6 cut(s) 481, 925, 2071, 2731, 3010, 3315
AjuI GAANNNNNNNTTGG 2 cut(s) 2643, 2675
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
Alw21I GWGCWC 1 cut(s) 3463
Alw26I GTCTC 2 cut(s) 237, 2472
Alw44I GTGCAC 1 cut(s) 3459
AlwI GGATC 5 cut(s) 530, 833, 1318, 1562, 2411
AlwNI CAGNNNCTG 1 cut(s) 796
Ama87I CYCGRG 2 cut(s) 2104, 2280
ApaI GGGCCC 1 cut(s) 3299
ApaLI GTGCAC 1 cut(s) 3459
ApeKI GCWGC 9 cut(s) 1008, 1700, 2670, 2919, 3029, 3032, 3035, 3055, 3058
ApoI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2132
ArsI GACNNNNNNTTYG 2 cut(s) 2647, 2679
Asp718I GGTACC 1 cut(s) 3261
AspA2I CCTAGG 1 cut(s) 3440
AspLEI GCGC 4 cut(s) 1917, 2934, 3099, 3252
AspS9I GGNCC 5 cut(s) 1346, 2048, 3195, 3295, 3296
AsuC2I CCSGG 1 cut(s) 3041
AvaI CYCGRG 2 cut(s) 2104, 2280
AvaII GGWCC 2 cut(s) 1346, 2048
AvrII CCTAGG 1 cut(s) 3440
BaeGI GKGCMC 2 cut(s) 3299, 3463
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BalI TGGCCA 4 cut(s) 1245, 1401, 1785, 2243
BanI GGYRCC 4 cut(s) 1417, 1427, 3261, 3428
BanII GRGCYC 2 cut(s) 2712, 3299
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BauI CACGAG 2 cut(s) 264, 3173
BbrPI CACGTG 2 cut(s) 3350, 3368
BbsI GAAGAC 3 cut(s) 1524, 2588, 2733
Bbv12I GWGCWC 1 cut(s) 3463
BbvCI CCTCAGC 1 cut(s) 2205
BbvI GCAGC 9 cut(s) 1020, 1687, 2657, 2931, 3041, 3044, 3047, 3067, 3070
BccI CCATC 5 cut(s) 284, 305, 655, 1703, 2480
BceAI ACGGC 1 cut(s) 2473
BciT130I CCWGG 6 cut(s) 483, 927, 2073, 2733, 3012, 3317
BciVI GTATCC 2 cut(s) 3337, 3398
BclI TGATCA 1 cut(s) 405
BcnI CCSGG 1 cut(s) 3041
BcoDI GTCTC 2 cut(s) 237, 2472
BcuI ACTAGT 1 cut(s) 1993
BfmI CTRYAG 6 cut(s) 702, 1253, 1372, 1662, 3053, 3127
BfrI CTTAAG 2 cut(s) 299, 1336
BfuAI ACCTGC 2 cut(s) 2969, 3060
BfuI GTATCC 2 cut(s) 3337, 3398
BglII AGATCT 2 cut(s) 2332, 2585
BlnI CCTAGG 1 cut(s) 3440
BlpI GCTNAGC 1 cut(s) 1406
BmcAI AGTACT 2 cut(s) 510, 2687
Bme1390I CCNGG 7 cut(s) 483, 927, 2073, 2733, 3012, 3041, 3317
Bme18I GGWCC 2 cut(s) 1346, 2048
BmeT110I CYCGRG 2 cut(s) 2104, 2280
BmgT120I GGNCC 5 cut(s) 1346, 2048, 3195, 3295, 3296
BmiI GGNNCC 6 cut(s) 1419, 1429, 1644, 3263, 3297, 3430
BmrFI CCNGG 7 cut(s) 483, 927, 2073, 2733, 3012, 3041, 3317
BmrI ACTGGG 1 cut(s) 2559
BmsI GCATC 8 cut(s) 568, 842, 971, 1455, 1477, 1687, 2819, 2956
BmuI ACTGGG 1 cut(s) 2559
BpiI GAAGAC 3 cut(s) 1524, 2588, 2733
BpmI CTGGAG 2 cut(s) 2454, 3009
Bpu10I CCTNAGC 1 cut(s) 2205
Bpu1102I GCTNAGC 1 cut(s) 1406
BpuEI CTTGAG 2 cut(s) 1054, 1758
BpuMI CCSGG 1 cut(s) 3041
BsaAI YACGTR 2 cut(s) 3350, 3368
BsaBI GATNNNNATC 3 cut(s) 294, 2225, 2540
BsaJI CCNNGG 6 cut(s) 1638, 2119, 2731, 3010, 3039, 3440
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 8 cut(s) 1168, 1198, 2888, 2918, 3364, 3394, 3421, 3451
Bsc4I CCNNNNNNNGG 7 cut(s) 545, 1355, 1939, 3272, 3316, 3369, 3442
Bse118I RCCGGY 1 cut(s) 3118
Bse1I ACTGG 8 cut(s) 335, 394, 623, 1348, 1402, 1682, 1804, 2554
Bse3DI GCAATG 5 cut(s) 57, 1018, 1567, 1833, 2422
Bse8I GATNNNNATC 3 cut(s) 294, 2225, 2540
BseBI CCWGG 6 cut(s) 483, 927, 2073, 2733, 3012, 3317
BseDI CCNNGG 6 cut(s) 1638, 2119, 2731, 3010, 3039, 3440
BseGI GGATG 6 cut(s) 316, 583, 986, 1473, 1948, 2686
BseJI GATNNNNATC 3 cut(s) 294, 2225, 2540
BseLI CCNNNNNNNGG 7 cut(s) 545, 1355, 1939, 3272, 3316, 3369, 3442
BseMI GCAATG 5 cut(s) 57, 1018, 1567, 1833, 2422
BseMII CTCAG 7 cut(s) 503, 1008, 1397, 2196, 2348, 2511, 3144
BseNI ACTGG 8 cut(s) 335, 394, 623, 1348, 1402, 1682, 1804, 2554
BseRI GAGGAG 2 cut(s) 730, 2091
BseSI GKGCMC 2 cut(s) 3299, 3463
BseXI GCAGC 9 cut(s) 1020, 1687, 2657, 2931, 3041, 3044, 3047, 3067, 3070
BsgI GTGCAG 1 cut(s) 3097
Bsh1236I CGCG 1 cut(s) 3097
BshNI GGYRCC 4 cut(s) 1417, 1427, 3261, 3428
BsiHKAI GWGCWC 1 cut(s) 3463
BsiHKCI CYCGRG 2 cut(s) 2104, 2280
BsiSI CCGG 3 cut(s) 947, 3041, 3119
BslFI GGGAC 4 cut(s) 2886, 2957, 3291, 3300
BslI CCNNNNNNNGG 7 cut(s) 545, 1355, 1939, 3272, 3316, 3369, 3442
BsmAI GTCTC 2 cut(s) 237, 2472
BsmFI GGGAC 4 cut(s) 2886, 2957, 3291, 3300
BsmI GAATGC 1 cut(s) 1103
BsoBI CYCGRG 2 cut(s) 2104, 2280
Bsp120I GGGCCC 1 cut(s) 3295
Bsp1286I GDGCHC 3 cut(s) 2712, 3299, 3463
Bsp1407I TGTACA 1 cut(s) 2719
Bsp1720I GCTNAGC 1 cut(s) 1406
BspACI CCGC 8 cut(s) 1425, 2811, 2993, 3086, 3095, 3259, 3307, 3328
BspCNI CTCAG 7 cut(s) 504, 1009, 1398, 2197, 2349, 2510, 3145
BspFNI CGCG 1 cut(s) 3097
BspHI TCATGA 1 cut(s) 3217
BspLI GGNNCC 6 cut(s) 1419, 1429, 1644, 3263, 3297, 3430
BspMAI CTGCAG 2 cut(s) 3057, 3131
BspMI ACCTGC 2 cut(s) 2969, 3060
BspPI GGATC 5 cut(s) 530, 833, 1318, 1562, 2411
BspT107I GGYRCC 4 cut(s) 1417, 1427, 3261, 3428
BspTI CTTAAG 2 cut(s) 299, 1336
BsrBI CCGCTC 1 cut(s) 3259
BsrDI GCAATG 5 cut(s) 57, 1018, 1567, 1833, 2422
BsrFI RCCGGY 1 cut(s) 3118
BsrGI TGTACA 1 cut(s) 2719
BsrI ACTGG 8 cut(s) 335, 394, 623, 1348, 1402, 1682, 1804, 2554
BssAI RCCGGY 1 cut(s) 3118
BssECI CCNNGG 6 cut(s) 1638, 2119, 2731, 3010, 3039, 3440
BssSI CACGAG 2 cut(s) 264, 3173
BssT1I CCWWGG 3 cut(s) 1638, 2119, 3440
Bst2BI CACGAG 2 cut(s) 264, 3173
Bst2UI CCWGG 6 cut(s) 483, 927, 2073, 2733, 3012, 3317
Bst4CI ACNGT 6 cut(s) 86, 223, 1124, 1241, 1530, 2900
Bst6I CTCTTC 4 cut(s) 518, 617, 1143, 2358
BstAFI CTTAAG 2 cut(s) 299, 1336
BstAUI TGTACA 1 cut(s) 2719
BstBAI YACGTR 2 cut(s) 3350, 3368
BstC8I GCNNGC 4 cut(s) 234, 1403, 2954, 3095
BstF5I GGATG 6 cut(s) 316, 583, 986, 1473, 1948, 2686
BstFNI CGCG 1 cut(s) 3097
BstHHI GCGC 4 cut(s) 1917, 2934, 3099, 3252
BstMAI GTCTC 2 cut(s) 237, 2472
BstNI CCWGG 6 cut(s) 483, 927, 2073, 2733, 3012, 3317
BstNSI RCATGY 3 cut(s) 873, 1194, 1558
BstSCI CCNGG 7 cut(s) 481, 925, 2071, 2731, 3010, 3039, 3315
BstSFI CTRYAG 6 cut(s) 702, 1253, 1372, 1662, 3053, 3127
BstSLI GKGCMC 2 cut(s) 3299, 3463
BstUI CGCG 1 cut(s) 3097
BstV1I GCAGC 9 cut(s) 1020, 1687, 2657, 2931, 3041, 3044, 3047, 3067, 3070
BstV2I GAAGAC 3 cut(s) 1524, 2588, 2733
BstX2I RGATCY 4 cut(s) 838, 2332, 2403, 2585
BstYI RGATCY 4 cut(s) 838, 2332, 2403, 2585
BsuI GTATCC 2 cut(s) 3337, 3398
BtgZI GCGATG 2 cut(s) 2842, 2933
BtsCI GGATG 6 cut(s) 316, 583, 986, 1473, 1948, 2686
BtsI GCAGTG 4 cut(s) 862, 1381, 2942, 2983
BtsIMutI CAGTG 8 cut(s) 387, 862, 1341, 1381, 2547, 2896, 2942, 2983
BveI ACCTGC 2 cut(s) 2969, 3060
Cac8I GCNNGC 4 cut(s) 234, 1403, 2954, 3095
CaiI CAGNNNCTG 1 cut(s) 796
CciI TCATGA 1 cut(s) 3217
CfoI GCGC 4 cut(s) 1917, 2934, 3099, 3252
Cfr10I RCCGGY 1 cut(s) 3118
Cfr13I GGNCC 5 cut(s) 1346, 2048, 3195, 3295, 3296
CseI GACGC 1 cut(s) 2959
Csp6I GTAC 9 cut(s) 174, 509, 616, 1770, 1881, 1967, 2686, 2720, 3262
CviQI GTAC 9 cut(s) 174, 509, 616, 1770, 1881, 1967, 2686, 2720, 3262
DraI TTTAAA 2 cut(s) 1600, 1764
DraIII CACNNNGTG 2 cut(s) 3353, 3458
EaeI YGGCCR 5 cut(s) 1243, 1399, 1783, 2241, 2741
Eam1104I CTCTTC 4 cut(s) 518, 617, 1143, 2358
EarI CTCTTC 4 cut(s) 518, 617, 1143, 2358
Eco130I CCWWGG 3 cut(s) 1638, 2119, 3440
Eco24I GRGCYC 2 cut(s) 2712, 3299
Eco47I GGWCC 2 cut(s) 1346, 2048
Eco57I CTGAAG 3 cut(s) 1208, 1538, 2409
Eco72I CACGTG 2 cut(s) 3350, 3368
Eco88I CYCGRG 2 cut(s) 2104, 2280
EcoRI GAATTC 1 cut(s) 2132
EcoRII CCWGG 6 cut(s) 481, 925, 2071, 2731, 3010, 3315
EcoT14I CCWWGG 3 cut(s) 1638, 2119, 3440
EcoT22I ATGCAT 3 cut(s) 871, 2482, 2611
EcoT38I GRGCYC 2 cut(s) 2712, 3299
ErhI CCWWGG 3 cut(s) 1638, 2119, 3440
FalI AAGNNNNNCTT 2 cut(s) 310, 342
FaqI GGGAC 4 cut(s) 2886, 2957, 3291, 3300
FauI CCCGC 3 cut(s) 2818, 3000, 3102
FbaI TGATCA 1 cut(s) 405
FokI GGATG 6 cut(s) 323, 590, 993, 1460, 1955, 2693
FriOI GRGCYC 2 cut(s) 2712, 3299
GlaI GCGC 4 cut(s) 1916, 2933, 3098, 3251
GsuI CTGGAG 2 cut(s) 2454, 3009
HapII CCGG 3 cut(s) 947, 3041, 3119
HgaI GACGC 1 cut(s) 2959
HhaI GCGC 4 cut(s) 1917, 2934, 3099, 3252
Hin6I GCGC 4 cut(s) 1915, 2932, 3097, 3250
HinP1I GCGC 4 cut(s) 1915, 2932, 3097, 3250
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HindIII AAGCTT 3 cut(s) 1134, 2066, 2251
HpaII CCGG 3 cut(s) 947, 3041, 3119
Hpy166II GTNNAC 7 cut(s) 6, 712, 944, 996, 2056, 2720, 3461
Hpy8I GTNNAC 7 cut(s) 6, 712, 944, 996, 2056, 2720, 3461
HpyAV CCTTC 7 cut(s) 606, 808, 1905, 2844, 3089, 3240, 3314
HpyCH4III ACNGT 6 cut(s) 86, 223, 1124, 1241, 1530, 2900
HpyCH4IV ACGT 4 cut(s) 3289, 3349, 3367, 3457
HpySE526I ACGT 4 cut(s) 3289, 3349, 3367, 3457
HspAI GCGC 4 cut(s) 1915, 2932, 3097, 3250
KpnI GGTACC 1 cut(s) 3265
Ksp22I TGATCA 1 cut(s) 405
LmnI GCTCC 1 cut(s) 3256
Lsp1109I GCAGC 9 cut(s) 1020, 1687, 2657, 2931, 3041, 3044, 3047, 3067, 3070
LweI GCATC 8 cut(s) 568, 842, 971, 1455, 1477, 1687, 2819, 2956
MaeII ACGT 4 cut(s) 3289, 3349, 3367, 3457
MbiI CCGCTC 1 cut(s) 3259
MflI RGATCY 4 cut(s) 838, 2332, 2403, 2585
MhlI GDGCHC 3 cut(s) 2712, 3299, 3463
MlsI TGGCCA 4 cut(s) 1245, 1401, 1785, 2243
MluNI TGGCCA 4 cut(s) 1245, 1401, 1785, 2243
MlyI GAGTC 4 cut(s) 713, 1640, 2252, 2602
MmeI TCCRAC 7 cut(s) 169, 488, 1175, 1595, 2819, 3002, 3072
Mox20I TGGCCA 4 cut(s) 1245, 1401, 1785, 2243
Mph1103I ATGCAT 3 cut(s) 871, 2482, 2611
MscI TGGCCA 4 cut(s) 1245, 1401, 1785, 2243
MslI CAYNNNNRTG 2 cut(s) 491, 3173
Msp20I TGGCCA 4 cut(s) 1245, 1401, 1785, 2243
MspA1I CMGCKG 2 cut(s) 1405, 2670
MspCI CTTAAG 2 cut(s) 299, 1336
MspI CCGG 3 cut(s) 947, 3041, 3119
MspR9I CCNGG 7 cut(s) 483, 927, 2073, 2733, 3012, 3041, 3317
Mva1269I GAATGC 1 cut(s) 1103
MvaI CCWGG 6 cut(s) 483, 927, 2073, 2733, 3012, 3317
MvnI CGCG 1 cut(s) 3097
NciI CCSGG 1 cut(s) 3041
NlaIV GGNNCC 6 cut(s) 1419, 1429, 1644, 3263, 3297, 3430
NmeAIII GCCGAG 1 cut(s) 3021
NmuCI GTSAC 9 cut(s) 1022, 1203, 1213, 1363, 1438, 1546, 1632, 2663, 2751
NsiI ATGCAT 3 cut(s) 871, 2482, 2611
NspI RCATGY 3 cut(s) 873, 1194, 1558
PaeR7I CTCGAG 1 cut(s) 2104
PagI TCATGA 1 cut(s) 3217
PaqCI CACCTGC 1 cut(s) 2969
PciI ACATGT 2 cut(s) 1190, 1554
PcsI WCGNNNNNNNCGW 1 cut(s) 3454
PctI GAATGC 1 cut(s) 1103
PflMI CCANNNNNTGG 1 cut(s) 1355
PfoI TCCNGGA 1 cut(s) 2071
PleI GAGTC 4 cut(s) 713, 1639, 2252, 2601
PmaCI CACGTG 2 cut(s) 3350, 3368
PmlI CACGTG 2 cut(s) 3350, 3368
PpsI GAGTC 4 cut(s) 713, 1639, 2252, 2601
Ppu21I YACGTR 2 cut(s) 3350, 3368
PscI ACATGT 2 cut(s) 1190, 1554
PsiI TTATAA 2 cut(s) 3186, 3231
Psp6I CCWGG 6 cut(s) 481, 925, 2071, 2731, 3010, 3315
PspCI CACGTG 2 cut(s) 3350, 3368
PspGI CCWGG 6 cut(s) 481, 925, 2071, 2731, 3010, 3315
PspN4I GGNNCC 6 cut(s) 1419, 1429, 1644, 3263, 3297, 3430
PspOMI GGGCCC 1 cut(s) 3295
PspPI GGNCC 5 cut(s) 1346, 2048, 3195, 3295, 3296
PstI CTGCAG 2 cut(s) 3057, 3131
PstNI CAGNNNCTG 1 cut(s) 796
PsuI RGATCY 4 cut(s) 838, 2332, 2403, 2585
PvuII CAGCTG 2 cut(s) 1405, 2670
RsaI GTAC 9 cut(s) 175, 510, 617, 1771, 1882, 1968, 2687, 2721, 3263
RsaNI GTAC 9 cut(s) 174, 509, 616, 1770, 1881, 1967, 2686, 2720, 3262
RseI CAYNNNNRTG 2 cut(s) 491, 3173
Sau96I GGNCC 5 cut(s) 1346, 2048, 3195, 3295, 3296
ScaI AGTACT 2 cut(s) 510, 2687
SchI GAGTC 4 cut(s) 713, 1640, 2252, 2602
ScrFI CCNGG 7 cut(s) 483, 927, 2073, 2733, 3012, 3041, 3317
SduI GDGCHC 3 cut(s) 2712, 3299, 3463
SfaNI GCATC 8 cut(s) 568, 842, 971, 1455, 1477, 1687, 2819, 2956
SfcI CTRYAG 6 cut(s) 702, 1253, 1372, 1662, 3053, 3127
Sfr274I CTCGAG 1 cut(s) 2104
SgrAI CRCCGGYG 1 cut(s) 3118
SinI GGWCC 2 cut(s) 1346, 2048
SlaI CTCGAG 1 cut(s) 2104
SmiMI CAYNNNNRTG 2 cut(s) 491, 3173
SmlI CTYRAG 5 cut(s) 299, 1033, 1336, 1773, 2104
SmoI CTYRAG 5 cut(s) 299, 1033, 1336, 1773, 2104
SpeI ACTAGT 1 cut(s) 1993
SsiI CCGC 8 cut(s) 1425, 2811, 2993, 3086, 3095, 3259, 3307, 3328
SspI AATATT 1 cut(s) 1390
StyD4I CCNGG 7 cut(s) 481, 925, 2071, 2731, 3010, 3039, 3315
StyI CCWWGG 3 cut(s) 1638, 2119, 3440
TaaI ACNGT 6 cut(s) 86, 223, 1124, 1241, 1530, 2900
TaiI ACGT 4 cut(s) 3292, 3352, 3370, 3460
TaqI TCGA 6 cut(s) 209, 608, 1688, 2105, 2185, 2927
TatI WGTACW 4 cut(s) 508, 1769, 2685, 2719
TauI GCSGC 1 cut(s) 1428
TscAI CASTG 8 cut(s) 394, 862, 1348, 1388, 2554, 2903, 2949, 2990
TseFI GTSAC 9 cut(s) 1022, 1203, 1213, 1363, 1438, 1546, 1632, 2663, 2751
TseI GCWGC 9 cut(s) 1008, 1700, 2670, 2919, 3029, 3032, 3035, 3055, 3058
Tsp45I GTSAC 9 cut(s) 1022, 1203, 1213, 1363, 1438, 1546, 1632, 2663, 2751
TspGWI ACGGA 1 cut(s) 1967
TspRI CASTG 8 cut(s) 394, 862, 1348, 1388, 2554, 2903, 2949, 2990
Van91I CCANNNNNTGG 1 cut(s) 1355
Vha464I CTTAAG 2 cut(s) 299, 1336
VneI GTGCAC 1 cut(s) 3459
VpaK11BI GGWCC 2 cut(s) 1346, 2048
XapI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2132
XbaI TCTAGA 2 cut(s) 1705, 2309
XceI RCATGY 3 cut(s) 873, 1194, 1558
XcmI CCANNNNNNNNNTGG 2 cut(s) 1090, 2443
XhoI CTCGAG 1 cut(s) 2104
XmaJI CCTAGG 1 cut(s) 3440
ZrmI AGTACT 2 cut(s) 510, 2687
Zsp2I ATGCAT 3 cut(s) 871, 2482, 2611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.