Rh2CG535800

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000412
Physical Location & Seq
Reverse (-)
71256575 .. 71261883
5309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG535800.1

Sequence Viewer

Length: 3063 bp
ATGTTCACTAACTTCTTTCTGTATTCATTCTTGTTATGCACTTTTCTTCATTGTTGCATTGCTAAAGATACAATAGCATATAACAGTTTGATTAGTGATGACAAAGGTGATACTATTGTTTCATCAGGAGAAAAATTTGAACTTGGGTTCTTTACTTCTAATGGAAGCTCTGGTACTAGAAGATATGTTGGAATTTGGTATTACAGATCGAATCCACAGACTGTTGTGTGGCTTGCAAATAGAGACAACCCTCTTTCAGATACTCGTGGAGTTTTCACTATTGCAGAAGATGGAAATCTTAAGGTGCTGGATGGCAAAAGAAAAACCTACTGGTCATCAAGTCTTGAAAGTTCATCATCAACAATTAGGACAGCAAAGCTCATGGACACTGGTAATCTTGTTGTGATCAATAGAGAGCAAGGAAACAACTCGGTTCAAATTGTCTGGCAGAGTTTTGAAAATCCAACCGACACATTTCTTCCTGGCATGAAAATGAGCGGAAAATTAGCACTGAGTTCATGGAAGAGTTACAATGATCCAGCAACAGGAAATTTCACATTTCAGCAAGACCAAGAGGATGCGAACCACTTTGTTATCTGGAAAAGATCAAGAAGGTACTGGAAGAGTGAAGATTATGGAAACTTCATTAGTTCTGATGAGATGGCTTCTGCAATTCTCTACTTGCTATCAAACTTCACATCTACAGCAGTCCACAATGACTCTGTGCCATATCTCACATCCTCATTATACACTTCTACAAGGCTGGTTATGAGTTTTTCAGGCCAGATTCAGTATCTGATGTGGGATAGTGAGAAGGTTTGGTCTTTGATATGGGCAGATCCTAGAGATAGATGCAGTGTGTATAATGCATGTGGAAATTTTGGTAGCTGTAACAGCAAAAATGGTTTGGTTTGCAAGTGTTTGCCTGGTTTTAAGCCTAGTTCACCGGATAATTGGAATCATGGAGATTATTCGGGCGGATGCACTAGGAAGTCAACATTATGTGGCAACAATGCTGAGAGTGACACATTCTTGAGCTTAAAGATGATGAAAGTGGGAAATCCAGATTCCCAATTTAATGCCAAAAGTGAAGTGGAATGCAGAGTAGAGTGCCTTAACAACTGTGAGTGTCAAGCTTATTTCTATGAAGAGGTGGGGAACAAAAAAACGGGTGGGAGTAGTAGTTCAACATGTTGGATTTGGTCACAAGATGTCACCAATCTTCAGGAGGACTATGACGGTGGCCAGAATCTACAGGTTCGTGTGGCAGTTTCAGATATAGAATCAACAGCGAGGAGCTGTGGAAGTTGTGGCACAAACCTGATCCCTTATCCCTTAAGCACTGGTCCAAAGTGTGGTGATGTCACCTACTATAGTTTCCACTGCAATATTTCAACCGGCCAGCTGAGCTTTGAGGCACCAAGCGGCACCTACCATGTCACAAGCATCAACGCAGACACACAAACATTTGTCATCCAAGCTAATGATGCAGATGAATGTAGAGATAAAAAATTTCTGAAGCTCATCCATTCTTCTCCATATAATGTGACAAACATGTGCAATGCTGATCCGACCCGTTTTAGTCCTGATTTGTCATTTAAAGGAGGATATGAAGTTGAAGTTGCTTGGGAGTCACCCTTGGAACCACCTTGTTCCTCATCTACAGACTGCAAGGACTGGCCTCGTTCGATATGTGATGCTGCTCTAGATGGGAAGAATAGGTGTCTTTGCCCTGCAAACTCAAAATGGGATAGCAGGAGTTTAAATTGTACTCAAGAAGTTGGCCACATAAAGCAAACTGGTGAGCAAGGGAAGATGACCCTAGCTCTAATCATTGCAGTAACTTGTATAAGTGTAGCTGTTCTAGCAATTCTGTCAAGTACCTTTGTTTATGCTTATATATGGAGAAGAAGGCGCATTAAGACACAAGAAGGCAGGGCAAATCTTCAAAAGTGTTCAACACTCAATCACTTTTATGACTGTGAGAGAAAAGTCAAGAACTTGATTGAATCAGGCCGATTTAAGGATGACGATACGGAGGGCATTGATGTACCCTCTTTTGATTTGGAAAGCATACTGGTAGCTACAACATACTTCTCCAATGCAAATAAACTTGGACAAGGAGGATTTGGTCCTGTTTACAAGGGTAAGCTTCCGGGAGGAGAAGAAATCGCTGTAAAGAGGCTCTCGAGTTGTTCAGGCCAAGGCTTAGAGGAATTCAAAAATGAAGTTTTGTTAATTGCCAAACTTCAACATCGGAATCTGGTTCGACTTTTGGGCTATTGTGCTGAGGGAGATGAAAAGATGTTAATCTATGAATACATGGCCAACAAAAGCTTAGACTCTTTCATCTTTGATCGAAAAGTATGTGTATCATTGGACTGGAATACACGCTTTAACATCATCTTAGGAATTGCTCGGGGGCTTCTTTATCTTCACCAAGATTCTAGATTAAGGGTTATTCATAGAGATCTGAAAACCAGCAACATTCTACTGAGTGAAGAGATGAACCCCAAAATATCAGACTTCGGTTTGGCAAGGATCTTTGGAGGCAATGAAACTTCAGCAAACACCAATAGAGTAGTGGGAACATACGGCTATATGTCTCCAGAGTATGCATTAGATGGGTTATTCTCAGTAAAATCTGATGTTTTTAGCTTTGGTGTAGTTGTGATTGAAATCATCACTGGGAAAAGGAACACAGGATTTTATCAGCCTGAAAGATCTTTGAGTCTTCTTGGCTATGCATGGCATTTGTGGAAAGAACAAAAGGCATTGGATTTGCTAGAACAAACACTTGGTCACAGCTGCAACAAGGATGAGTATTTCAAGTGTGTTAATGTTGGGCTCTTATGTGTACAAGAAGATCCAGGTGATCGGCCAACCATGTCACAAGTGGTTTTCATGCTCGGAAGTGAAACTGCAACAATTCCAACCCCCAAACAACCCGCTTTCGTTGTTAGGCGATGCCCTTCTAGTTCTAGCAGGGCTTCTAATTCTTCAAGCAAACCAGAAACGGTTTCGAACAATGGGTTAACTGTCACCTTAGAAGATGGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1020

Amino Acids

113.79

Weight (kDa)

6.06

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 168 1.2e-29 D-mannose binding lectin
S_locus_glycop PF00954 214 - 314 1.9e-22 S-locus glycoprotein domain
PAN_2 PF08276 338 - 407 4e-10 PAN-like domain
GUB_WAK_bind PF13947 435 - 492 3e-06 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 700 - 966 6.7e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 702 - 968 2.1e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 972 - 1020 7.2e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1417, 1427
AccB7I CCANNNNNTGG 1 cut(s) 1355
AccBSI CCGCTC 1 cut(s) 498
AciI CCGC 4 cut(s) 498, 978, 1425, 2949
AclWI GGATC 6 cut(s) 530, 833, 1318, 1562, 2549, 2861
AcoI YGGCCR 5 cut(s) 1243, 1399, 1783, 2325, 2879
AcsI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2216
AcuI CTGAAG 3 cut(s) 1208, 1538, 2547
AfaI GTAC 6 cut(s) 175, 617, 1771, 1882, 2052, 2859
AfiI CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
AflII CTTAAG 2 cut(s) 299, 1336
AflIII ACRYGT 2 cut(s) 1190, 1554
AjnI CCWGG 3 cut(s) 481, 925, 2869
AjuI GAANNNNNNNTTGG 2 cut(s) 2781, 2813
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
Alw26I GTCTC 2 cut(s) 237, 2610
AlwI GGATC 6 cut(s) 530, 833, 1318, 1562, 2549, 2861
AlwNI CAGNNNCTG 1 cut(s) 796
Ama87I CYCGRG 2 cut(s) 2188, 2418
AoxI GGCC 9 cut(s) 781, 1243, 1399, 1679, 1783, 2014, 2200, 2325, 2879
ApeKI GCWGC 2 cut(s) 1700, 2808
ApoI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2216
ArsI GACNNNNNNTTYG 2 cut(s) 2785, 2817
AspLEI GCGC 1 cut(s) 1917
AspS9I GGNCC 2 cut(s) 1346, 2132
AsuC2I CCSGG 1 cut(s) 2157
AsuII TTCGAA 1 cut(s) 3023
AvaI CYCGRG 2 cut(s) 2188, 2418
AvaII GGWCC 2 cut(s) 1346, 2132
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BalI TGGCCA 3 cut(s) 1245, 1785, 2327
BanI GGYRCC 2 cut(s) 1417, 1427
BanII GRGCYC 1 cut(s) 2850
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 2726
BbvCI CCTCAGC 1 cut(s) 2289
BbvI GCAGC 2 cut(s) 1687, 2795
BccI CCATC 6 cut(s) 284, 305, 655, 1703, 2618, 3047
BceAI ACGGC 1 cut(s) 2611
BciT130I CCWGG 3 cut(s) 483, 927, 2871
BclI TGATCA 1 cut(s) 405
BcnI CCSGG 1 cut(s) 2157
BcoDI GTCTC 2 cut(s) 237, 2610
BfmI CTRYAG 4 cut(s) 702, 1253, 1372, 1662
BfrI CTTAAG 2 cut(s) 299, 1336
BglII AGATCT 2 cut(s) 2470, 2723
BisI GCNGC 3 cut(s) 1426, 1701, 2809
BlpI GCTNAGC 1 cut(s) 1406
BlsI GCNGC 3 cut(s) 1427, 1702, 2810
Bme1390I CCNGG 4 cut(s) 483, 927, 2157, 2871
Bme18I GGWCC 2 cut(s) 1346, 2132
BmeT110I CYCGRG 2 cut(s) 2188, 2418
BmgT120I GGNCC 2 cut(s) 1346, 2132
BmiI GGNNCC 3 cut(s) 1419, 1429, 1644
BmrFI CCNGG 4 cut(s) 483, 927, 2157, 2871
BmrI ACTGGG 1 cut(s) 2697
BmsI GCATC 7 cut(s) 568, 842, 971, 1455, 1477, 1687, 2957
BmuI ACTGGG 1 cut(s) 2697
BpiI GAAGAC 1 cut(s) 2726
BpmI CTGGAG 1 cut(s) 2592
Bpu10I CCTNAGC 1 cut(s) 2289
Bpu1102I GCTNAGC 1 cut(s) 1406
Bpu14I TTCGAA 1 cut(s) 3023
BpuEI CTTGAG 2 cut(s) 1054, 1758
BpuMI CCSGG 1 cut(s) 2157
BsaBI GATNNNNATC 3 cut(s) 294, 2309, 2678
BsaJI CCNNGG 2 cut(s) 1638, 2203
BsaWI WCCGGW 1 cut(s) 946
BsaXI ACNNNNNCTCC 2 cut(s) 1168, 1198
Bsc4I CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
Bse118I RCCGGY 1 cut(s) 1397
Bse1I ACTGG 9 cut(s) 335, 394, 623, 1348, 1682, 1804, 2082, 2387, 2692
Bse3DI GCAATG 4 cut(s) 57, 1567, 1833, 2560
Bse8I GATNNNNATC 3 cut(s) 294, 2309, 2678
BseBI CCWGG 3 cut(s) 483, 927, 2871
BseDI CCNNGG 2 cut(s) 1638, 2203
BseGI GGATG 8 cut(s) 316, 583, 737, 986, 1473, 1524, 2032, 2824
BseJI GATNNNNATC 3 cut(s) 294, 2309, 2678
BseLI CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
BseMI GCAATG 4 cut(s) 57, 1567, 1833, 2560
BseMII CTCAG 6 cut(s) 503, 1008, 1397, 2280, 2486, 2649
BseNI ACTGG 9 cut(s) 335, 394, 623, 1348, 1682, 1804, 2082, 2387, 2692
BseRI GAGGAG 2 cut(s) 1309, 2175
BseXI GCAGC 2 cut(s) 1687, 2795
BshFI GGCC 9 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327, 2881
BshNI GGYRCC 2 cut(s) 1417, 1427
BsiHKCI CYCGRG 2 cut(s) 2188, 2418
BsiSI CCGG 3 cut(s) 947, 1398, 2156
BslI CCNNNNNNNGG 3 cut(s) 545, 1355, 2023
BsmAI GTCTC 2 cut(s) 237, 2610
BsmI GAATGC 1 cut(s) 1103
BsnI GGCC 9 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327, 2881
BsoBI CYCGRG 2 cut(s) 2188, 2418
Bsp119I TTCGAA 1 cut(s) 3023
Bsp1286I GDGCHC 1 cut(s) 2850
Bsp1407I TGTACA 1 cut(s) 2857
Bsp1720I GCTNAGC 1 cut(s) 1406
BspACI CCGC 4 cut(s) 498, 978, 1425, 2949
BspANI GGCC 9 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327, 2881
BspCNI CTCAG 6 cut(s) 504, 1009, 1398, 2281, 2487, 2648
BspLI GGNNCC 3 cut(s) 1419, 1429, 1644
BspPI GGATC 6 cut(s) 530, 833, 1318, 1562, 2549, 2861
BspT104I TTCGAA 1 cut(s) 3023
BspT107I GGYRCC 2 cut(s) 1417, 1427
BspTI CTTAAG 2 cut(s) 299, 1336
BsrBI CCGCTC 1 cut(s) 498
BsrDI GCAATG 4 cut(s) 57, 1567, 1833, 2560
BsrFI RCCGGY 1 cut(s) 1397
BsrGI TGTACA 1 cut(s) 2857
BsrI ACTGG 9 cut(s) 335, 394, 623, 1348, 1682, 1804, 2082, 2387, 2692
BssAI RCCGGY 1 cut(s) 1397
BssECI CCNNGG 2 cut(s) 1638, 2203
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 2 cut(s) 1638, 2203
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 483, 927, 2871
Bst4CI ACNGT 7 cut(s) 86, 223, 1124, 1241, 1982, 3019, 3040
Bst6I CTCTTC 4 cut(s) 518, 617, 1143, 2496
BstAFI CTTAAG 2 cut(s) 299, 1336
BstAUI TGTACA 1 cut(s) 2857
BstBI TTCGAA 1 cut(s) 3023
BstC8I GCNNGC 2 cut(s) 234, 1403
BstF5I GGATG 8 cut(s) 316, 583, 737, 986, 1473, 1524, 2032, 2824
BstHHI GCGC 1 cut(s) 1917
BstMAI GTCTC 2 cut(s) 237, 2610
BstMWI GCNNNNNNNGC 4 cut(s) 894, 1407, 1487, 1865
BstNI CCWGG 3 cut(s) 483, 927, 2871
BstNSI RCATGY 3 cut(s) 873, 1194, 1558
BstSCI CCNGG 4 cut(s) 481, 925, 2155, 2869
BstSFI CTRYAG 4 cut(s) 702, 1253, 1372, 1662
BstV1I GCAGC 2 cut(s) 1687, 2795
BstV2I GAAGAC 1 cut(s) 2726
BstX2I RGATCY 5 cut(s) 838, 2470, 2541, 2723, 2866
BstYI RGATCY 5 cut(s) 838, 2470, 2541, 2723, 2866
BsuRI GGCC 9 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327, 2881
BtgZI GCGATG 1 cut(s) 2980
BtsCI GGATG 8 cut(s) 316, 583, 737, 986, 1473, 1524, 2032, 2824
BtsI GCAGTG 2 cut(s) 862, 1381
BtsIMutI CAGTG 6 cut(s) 387, 509, 862, 1341, 1381, 2685
Cac8I GCNNGC 2 cut(s) 234, 1403
CaiI CAGNNNCTG 1 cut(s) 796
CfoI GCGC 1 cut(s) 1917
Cfr10I RCCGGY 1 cut(s) 1397
Cfr13I GGNCC 2 cut(s) 1346, 2132
Csp6I GTAC 6 cut(s) 174, 616, 1770, 1881, 2051, 2858
CviQI GTAC 6 cut(s) 174, 616, 1770, 1881, 2051, 2858
DraI TTTAAA 2 cut(s) 1600, 1764
EaeI YGGCCR 5 cut(s) 1243, 1399, 1783, 2325, 2879
Eam1104I CTCTTC 4 cut(s) 518, 617, 1143, 2496
EarI CTCTTC 4 cut(s) 518, 617, 1143, 2496
EciI GGCGGA 1 cut(s) 993
Eco130I CCWWGG 2 cut(s) 1638, 2203
Eco24I GRGCYC 1 cut(s) 2850
Eco47I GGWCC 2 cut(s) 1346, 2132
Eco57I CTGAAG 3 cut(s) 1208, 1538, 2547
Eco88I CYCGRG 2 cut(s) 2188, 2418
EcoRI GAATTC 1 cut(s) 2216
EcoRII CCWGG 3 cut(s) 481, 925, 2869
EcoT14I CCWWGG 2 cut(s) 1638, 2203
EcoT22I ATGCAT 3 cut(s) 871, 2620, 2749
EcoT38I GRGCYC 1 cut(s) 2850
ErhI CCWWGG 2 cut(s) 1638, 2203
FauI CCCGC 1 cut(s) 2956
FbaI TGATCA 1 cut(s) 405
Fnu4HI GCNGC 3 cut(s) 1426, 1701, 2809
FokI GGATG 8 cut(s) 323, 590, 724, 993, 1460, 1511, 2039, 2831
FriOI GRGCYC 1 cut(s) 2850
Fsp4HI GCNGC 3 cut(s) 1426, 1701, 2809
GlaI GCGC 1 cut(s) 1916
GluI GCNGC 3 cut(s) 1426, 1701, 2809
GsuI CTGGAG 1 cut(s) 2592
HaeIII GGCC 9 cut(s) 783, 1245, 1401, 1681, 1785, 2016, 2202, 2327, 2881
HapII CCGG 3 cut(s) 947, 1398, 2156
HhaI GCGC 1 cut(s) 1917
Hin6I GCGC 1 cut(s) 1915
HinP1I GCGC 1 cut(s) 1915
HincII GTYRAC 2 cut(s) 996, 3036
HindII GTYRAC 2 cut(s) 996, 3036
HindIII AAGCTT 3 cut(s) 1134, 2150, 2335
HpaI GTTAAC 1 cut(s) 3036
HpaII CCGG 3 cut(s) 947, 1398, 2156
Hpy166II GTNNAC 7 cut(s) 6, 712, 944, 996, 2140, 2858, 3036
Hpy8I GTNNAC 7 cut(s) 6, 712, 944, 996, 2140, 2858, 3036
HpyAV CCTTC 5 cut(s) 606, 808, 1905, 1925, 2982
HpyCH4III ACNGT 7 cut(s) 86, 223, 1124, 1241, 1982, 3019, 3040
HpyF10VI GCNNNNNNNGC 4 cut(s) 894, 1407, 1487, 1865
HspAI GCGC 1 cut(s) 1915
Ksp22I TGATCA 1 cut(s) 405
KspAI GTTAAC 1 cut(s) 3036
LmnI GCTCC 1 cut(s) 1296
Lsp1109I GCAGC 2 cut(s) 1687, 2795
LweI GCATC 7 cut(s) 568, 842, 971, 1455, 1477, 1687, 2957
MbiI CCGCTC 1 cut(s) 498
MflI RGATCY 5 cut(s) 838, 2470, 2541, 2723, 2866
MhlI GDGCHC 1 cut(s) 2850
MlsI TGGCCA 3 cut(s) 1245, 1785, 2327
MluNI TGGCCA 3 cut(s) 1245, 1785, 2327
MlyI GAGTC 4 cut(s) 713, 1640, 2336, 2740
MmeI TCCRAC 5 cut(s) 169, 488, 1175, 1595, 2957
Mox20I TGGCCA 3 cut(s) 1245, 1785, 2327
Mph1103I ATGCAT 3 cut(s) 871, 2620, 2749
MscI TGGCCA 3 cut(s) 1245, 1785, 2327
MslI CAYNNNNRTG 2 cut(s) 491, 1974
Msp20I TGGCCA 3 cut(s) 1245, 1785, 2327
MspA1I CMGCKG 2 cut(s) 1405, 2808
MspCI CTTAAG 2 cut(s) 299, 1336
MspI CCGG 3 cut(s) 947, 1398, 2156
MspR9I CCNGG 4 cut(s) 483, 927, 2157, 2871
Mva1269I GAATGC 1 cut(s) 1103
MvaI CCWGG 3 cut(s) 483, 927, 2871
MwoI GCNNNNNNNGC 4 cut(s) 894, 1407, 1487, 1865
NciI CCSGG 1 cut(s) 2157
NlaIV GGNNCC 3 cut(s) 1419, 1429, 1644
NsiI ATGCAT 3 cut(s) 871, 2620, 2749
NspI RCATGY 3 cut(s) 873, 1194, 1558
NspV TTCGAA 1 cut(s) 3023
PaeR7I CTCGAG 1 cut(s) 2188
PciI ACATGT 2 cut(s) 1190, 1554
PctI GAATGC 1 cut(s) 1103
PfeI GAWTC 9 cut(s) 211, 787, 958, 1067, 1249, 1283, 2009, 2260, 2444
PflMI CCANNNNNTGG 1 cut(s) 1355
PfoI TCCNGGA 1 cut(s) 2155
PkrI GCNGC 3 cut(s) 1427, 1702, 2810
PleI GAGTC 4 cut(s) 713, 1639, 2336, 2739
PpsI GAGTC 4 cut(s) 713, 1639, 2336, 2739
PscI ACATGT 2 cut(s) 1190, 1554
Psp6I CCWGG 3 cut(s) 481, 925, 2869
PspGI CCWGG 3 cut(s) 481, 925, 2869
PspN4I GGNNCC 3 cut(s) 1419, 1429, 1644
PspPI GGNCC 2 cut(s) 1346, 2132
PstNI CAGNNNCTG 1 cut(s) 796
PsuI RGATCY 5 cut(s) 838, 2470, 2541, 2723, 2866
PvuII CAGCTG 2 cut(s) 1405, 2808
RsaI GTAC 6 cut(s) 175, 617, 1771, 1882, 2052, 2859
RsaNI GTAC 6 cut(s) 174, 616, 1770, 1881, 2051, 2858
RseI CAYNNNNRTG 2 cut(s) 491, 1974
SatI GCNGC 3 cut(s) 1426, 1701, 2809
Sau96I GGNCC 2 cut(s) 1346, 2132
SchI GAGTC 4 cut(s) 713, 1640, 2336, 2740
ScrFI CCNGG 4 cut(s) 483, 927, 2157, 2871
SduI GDGCHC 1 cut(s) 2850
SfaNI GCATC 7 cut(s) 568, 842, 971, 1455, 1477, 1687, 2957
SfcI CTRYAG 4 cut(s) 702, 1253, 1372, 1662
Sfr274I CTCGAG 1 cut(s) 2188
SfuI TTCGAA 1 cut(s) 3023
SinI GGWCC 2 cut(s) 1346, 2132
SlaI CTCGAG 1 cut(s) 2188
SmiMI CAYNNNNRTG 2 cut(s) 491, 1974
SmlI CTYRAG 5 cut(s) 299, 1033, 1336, 1773, 2188
SmoI CTYRAG 5 cut(s) 299, 1033, 1336, 1773, 2188
SsiI CCGC 4 cut(s) 498, 978, 1425, 2949
SspI AATATT 1 cut(s) 1390
StyD4I CCNGG 4 cut(s) 481, 925, 2155, 2869
StyI CCWWGG 2 cut(s) 1638, 2203
TaaI ACNGT 7 cut(s) 86, 223, 1124, 1241, 1982, 3019, 3040
TaqI TCGA 7 cut(s) 209, 1688, 2189, 2269, 2359, 3023, 3058
TatI WGTACW 2 cut(s) 1769, 2857
TauI GCSGC 1 cut(s) 1428
TfiI GAWTC 9 cut(s) 211, 787, 958, 1067, 1249, 1283, 2009, 2260, 2444
TscAI CASTG 6 cut(s) 394, 516, 862, 1348, 1388, 2692
TseI GCWGC 2 cut(s) 1700, 2808
TspGWI ACGGA 1 cut(s) 2051
TspRI CASTG 6 cut(s) 394, 516, 862, 1348, 1388, 2692
Van91I CCANNNNNTGG 1 cut(s) 1355
Vha464I CTTAAG 2 cut(s) 299, 1336
VpaK11BI GGWCC 2 cut(s) 1346, 2132
XapI RAATTY 6 cut(s) 134, 192, 550, 877, 1511, 2216
XbaI TCTAGA 2 cut(s) 1705, 2447
XceI RCATGY 3 cut(s) 873, 1194, 1558
XcmI CCANNNNNNNNNTGG 3 cut(s) 1090, 2581, 2893
XhoI CTCGAG 1 cut(s) 2188
Zsp2I ATGCAT 3 cut(s) 871, 2620, 2749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.