Rroxscaffold_2G00089220

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000412
Physical Location & Seq
Forward (+)
11171584 .. 11175981
4398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089220.1

Sequence Viewer

Length: 2607 bp
ATGGTATCTTACGTCTTTTTCTTGTATGTAGTACTATTGCTGTGCGCTTATGACAACCTATTTTGTTCTGCTAGAGATACACTAACATATGACGATCCAGTTATCGATGATGGATCAGAAGCTCTAGTTTCAGCAGGAGGTGAGTTTGAACTGGGTTTCTTCTCTACCACTACGGGGAAAAGATATGTGGGCATATGGTATCATAAGGTCACTCCAAGAACAGTTGTATGGGTTGCTAACAGAGATGAACCTCTACCTGCCAATTCCATTGGAGTTCTTGCAATTGATGAAGGTAATCTCCGCGTGTTGGACAGTGTTACTCGAAAGCTTTATTGGTCAACAGATATTGGAAAATCTTCACCTTTTAACAAGACAGTGAAGATAATGGATTCAGGGAACCTAGTGTTAAGGGATACTGGTGAGTTGACAGCGGATCCTTTGTGGCAGAGCTTTCAATATCCAACTGATACATTCATTCCTGGGATGCGAATGGATAGTAACTTGAAGTTGACTTCATGGAGCGACGAAGATGACCGAAGAACTGGGAACTACACCTTCGGACTAGATCAAGAAGGGAAGTCAGAGAACCAGTTTACCATCTTAAATAATTCGTTTCCTTACTGGAAAAGTGGAGAGCCGGGAAAATATTTAAGCTCAGATGAGATGCGTCCTGAGGTAGCTTACTTGTTATCAAATTTCAGCAAGAATTCTTCTCTTTATACAACTCAGCTGCAATTTGATTACAATTATACAAGATTGGCGATGAATTCTACTGGAGTGCTCAATGGTTTGACTTGGAATGACACCATTAAGCAGTGGGTTGTGTTTTTCTCTGCGCCAAAAGATCAATGCAGTGTGATTAATGCTTGTGGAAACTTTGGCAGCTGCAATGTTGATAATACCCCGCTGGTGTGCAAATGTTTGCCAGGTTTCAACCCCCAGTTTTTAGAGAAATGGAAATATGGAGATTTTTCAGGTGGATGCAAAAGACAGTCGTACATATGTGGCAAGAATGAAACCTTCTTAAGCATAAAGGTGAACAAAGCGGGAAGCCCAGATTCATCGACCAATGTTACCGATGATACAGAATGCGAGAATAAGTGCCTTGGTAATTGCAACTGCCAGGCTTACTCATTTGACGTCACTGCTCGAAGGGATAATCTGATCACTTGTTATCAATGGTCCACGGATCTAAATAATCTTGTAGAGGAGTCTGGTGACAATGGCAAGAATCTCAATGCTCGAGTTCCCGTGCCAGATTTAGAATCAACTACAAGAGACTGCAACCCTTGTGGCACAACTACAATTCCTTATCCTCTGAGCACTAGAATAGATTGTGGCGACCCCACGTACAAGAGTTTCGCGTGCAATACCTCCATTAATGAATTTAGCTTCCTGGGACCAGATGGCCACTCCTTTAGAGTTATAAATATCAGCCGAAAAGGAAATCTACAAGAGCTATCAAATAAGAGTTCAAGGAAAAAATTGTTATTGTCTCTGGTTGTCGTGGCAGTCATAAGTGGGATTTTTCTGGCATGCATCATTGGTTTTTGGATATGGAGAAGAAAGATTACTGACAGACGAGATCAAGTAAGTCGTCCGCAGCTCGATAGTGAAAGACGAATGAAAGAACTCATAGACACGAGGGACTTTAATGAAGAAGATGACAAAGGCATAGATGTACCTTTTTTTGATTTGCAAAGCATACTAGACGCTACAGATAATTTCTCAATTGGAAACAAGCTTGGACAAGGGGGGTATGGGCCTGTTTACAAGGGGATCTTTCCTGGAGGTCAAGAAATTGCTGTAAAGAGGCTATCAAGAGTTTCGGGACAAGGCTTACAGGAGTTTAAGAATGAGGTGGTGCTAATCGCCAAACTTCAACATCGTAATCTTGTAAGACTCAGAGGATATTGCATGAAAGGAGAAGAAAAGATTTTACTCTATGAGTACATGCCTAACAAAAGCTTAGATTCCTTCATATTTGGACTTCTTTATCTTCATCAAGATTCCAGGTTGAGGATCATTCATAGAGATTTGAAAACCAGCAATGTTCTTTTGGATGAGGAGATGAACCCCAAAATATCTGACTTCGGCTTGGCCAGGATTGTTGGAGGCAAAGAAACAGAGGCAAACACAAATACAGTAGTAGGAACCTATGGTTACATGTCACCGGAATATGCATTGGATGGAAATTTCTCTGTCAAATCAGATGTCTTTAGTTTCGGTGTGGTTCTTCTAGAGATAATCAGCGGAAAAAAGAATGCAGGATTTTATCAGTCCAAACAAACTTTCAGTCTCCTTAGTTATACATGGGGACTCTGGACAGAAAACAAGGTGTCGGATTTAATGGACAACAATTTGGAAGAAAGTTGCAACAGAAGTGAATTTATAAAGTGTGTAAATGTTGGGCTCTTATGCGTACAAGAAGATCCGGTTGATCGCCCTACAATGTCAAATGTTGTTACCTTGCTTGACAGTGAAACTGCAATCCCACCAACTCCTAAACAACCAGCGTTTTTTATACGGAGAGGCAACTCTAGCACAGTTTCCTCTTCTACTAAAGCAGAAACATACTCTGAAATAACTACTCTGGAAGGTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

868

Amino Acids

97.46

Weight (kDa)

5.25

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 169 7.9e-25 D-mannose binding lectin
S_locus_glycop PF00954 214 - 314 9.8e-22 S-locus glycoprotein domain
PAN_2 PF08276 335 - 400 8.2e-10 PAN-like domain
Pkinase PF00069 577 - 661 1e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 578 - 661 6e-13 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 663 - 824 4e-24 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 663 - 822 5e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1427, 2393
AatII GACGTC 1 cut(s) 1143
Acc36I ACCTGC 1 cut(s) 265
AccII CGCG 2 cut(s) 303, 1364
AciI CCGC 6 cut(s) 301, 431, 905, 1046, 1601, 2253
AclWI GGATC 8 cut(s) 89, 121, 428, 441, 1197, 1787, 2030, 2426
AcoI YGGCCR 2 cut(s) 1408, 2100
AcsI RAATTY 6 cut(s) 694, 706, 766, 1385, 2194, 2387
AcyI GRCGYC 1 cut(s) 1140
AfaI GTAC 6 cut(s) 33, 998, 1352, 1683, 1952, 2424
AfiI CCNNNNNNNGG 3 cut(s) 174, 307, 2019
AflII CTTAAG 1 cut(s) 1024
AflIII ACRYGT 1 cut(s) 2166
AgsI TTSAA 7 cut(s) 149, 455, 505, 934, 1476, 1883, 2041
AjnI CCWGG 7 cut(s) 478, 925, 1122, 1395, 1786, 2012, 2102
AjuI GAANNNNNNNTTGG 4 cut(s) 316, 348, 2276, 2308
Alw21I GWGCWC 2 cut(s) 783, 1325
Alw26I GTCTC 3 cut(s) 1272, 1500, 2303
AlwI GGATC 8 cut(s) 89, 121, 428, 441, 1197, 1787, 2030, 2426
Ama87I CYCGRG 1 cut(s) 1242
AoxI GGCC 3 cut(s) 1408, 1763, 2100
ApeKI GCWGC 4 cut(s) 730, 882, 885, 1603
ApoI RAATTY 6 cut(s) 694, 706, 766, 1385, 2194, 2387
ArsI GACNNNNNNTTYG 4 cut(s) 2199, 2231, 2281, 2313
AseI ATTAAT 2 cut(s) 861, 1380
Asp700I GAANNNNTTC 1 cut(s) 355
AspLEI GCGC 2 cut(s) 47, 838
AspS9I GGNCC 3 cut(s) 1182, 1400, 1763
AsuC2I CCSGG 1 cut(s) 639
AsuHPI GGTGA 6 cut(s) 152, 351, 431, 1048, 1229, 2163
AvaI CYCGRG 1 cut(s) 1242
AvaII GGWCC 2 cut(s) 1182, 1400
AxyI CCTNAGG 1 cut(s) 672
BalI TGGCCA 2 cut(s) 1410, 2102
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 2415
BarI GAAGNNNNNNTAC 2 cut(s) 1555, 1587
BauI CACGAG 1 cut(s) 1642
Bbv12I GWGCWC 2 cut(s) 783, 1325
BbvI GCAGC 4 cut(s) 717, 872, 894, 1615
BccI CCATC 4 cut(s) 104, 605, 1400, 2183
BciT130I CCWGG 7 cut(s) 480, 927, 1124, 1397, 1788, 2014, 2104
BciVI GTATCC 1 cut(s) 406
BclI TGATCA 1 cut(s) 1164
BcnI CCSGG 1 cut(s) 639
BcoDI GTCTC 3 cut(s) 1272, 1500, 2303
BfaI CTAG 8 cut(s) 72, 125, 401, 563, 1326, 1709, 2240, 2541
BfmI CTRYAG 1 cut(s) 1716
BfrI CTTAAG 1 cut(s) 1024
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 4 cut(s) 731, 883, 886, 1604
BlsI GCNGC 4 cut(s) 732, 884, 887, 1605
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 8 cut(s) 480, 639, 927, 1124, 1397, 1788, 2014, 2104
Bme18I GGWCC 2 cut(s) 1182, 1400
BmeT110I CYCGRG 1 cut(s) 1242
BmgT120I GGNCC 3 cut(s) 1182, 1400, 1763
BmiI GGNNCC 4 cut(s) 398, 435, 1401, 2155
BmrFI CCNGG 8 cut(s) 480, 639, 927, 1124, 1397, 1788, 2014, 2104
BmrI ACTGGG 3 cut(s) 161, 552, 934
BmsI GCATC 4 cut(s) 474, 654, 971, 1548
BmuI ACTGGG 3 cut(s) 161, 552, 934
BpmI CTGGAG 2 cut(s) 795, 1809
BpuMI CCSGG 1 cut(s) 639
Bsa29I ATCGAT 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 1350
BsaHI GRCGYC 1 cut(s) 1140
BsaJI CCNNGG 4 cut(s) 479, 1105, 1185, 1396
BsaWI WCCGGW 2 cut(s) 2173, 2434
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 307, 2019
Bse1I ACTGG 8 cut(s) 98, 156, 421, 547, 589, 626, 778, 940
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 2 cut(s) 895, 2056
BseBI CCWGG 7 cut(s) 480, 927, 1124, 1397, 1788, 2014, 2104
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 4 cut(s) 479, 1105, 1185, 1396
BseGI GGATG 4 cut(s) 489, 986, 2068, 2194
BseLI CCNNNNNNNGG 3 cut(s) 174, 307, 2019
BseMI GCAATG 2 cut(s) 895, 2056
BseMII CTCAG 5 cut(s) 663, 669, 740, 1310, 1918
BseNI ACTGG 8 cut(s) 98, 156, 421, 547, 589, 626, 778, 940
BseRI GAGGAG 2 cut(s) 1223, 2081
BseXI GCAGC 4 cut(s) 717, 872, 894, 1615
Bsh1236I CGCG 2 cut(s) 303, 1364
BshFI GGCC 3 cut(s) 1410, 1765, 2102
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 783, 1325
BsiHKCI CYCGRG 1 cut(s) 1242
BsiSI CCGG 3 cut(s) 638, 2174, 2435
BslFI GGGAC 4 cut(s) 1413, 1661, 1845, 2331
BslI CCNNNNNNNGG 3 cut(s) 174, 307, 2019
BsmAI GTCTC 3 cut(s) 1272, 1500, 2303
BsmFI GGGAC 4 cut(s) 1413, 1661, 1845, 2331
BsmI GAATGC 2 cut(s) 1094, 2269
BsnI GGCC 3 cut(s) 1410, 1765, 2102
BsoBI CYCGRG 1 cut(s) 1242
Bsp1286I GDGCHC 3 cut(s) 783, 1325, 2415
BspACI CCGC 6 cut(s) 301, 431, 905, 1046, 1601, 2253
BspANI GGCC 3 cut(s) 1410, 1765, 2102
BspCNI CTCAG 5 cut(s) 664, 668, 739, 1311, 1917
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 2 cut(s) 303, 1364
BspLI GGNNCC 4 cut(s) 398, 435, 1401, 2155
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 8 cut(s) 89, 121, 428, 441, 1197, 1787, 2030, 2426
BspTI CTTAAG 1 cut(s) 1024
BsrDI GCAATG 2 cut(s) 895, 2056
BsrI ACTGG 8 cut(s) 98, 156, 421, 547, 589, 626, 778, 940
BssECI CCNNGG 4 cut(s) 479, 1105, 1185, 1396
BssNI GRCGYC 1 cut(s) 1140
BssSI CACGAG 1 cut(s) 1642
BssT1I CCWWGG 1 cut(s) 1105
Bst2BI CACGAG 1 cut(s) 1642
Bst2UI CCWGG 7 cut(s) 480, 927, 1124, 1397, 1788, 2014, 2104
Bst4CI ACNGT 7 cut(s) 223, 314, 376, 993, 2146, 2480, 2548
Bst6I CTCTTC 1 cut(s) 2560
BstACI GRCGYC 1 cut(s) 1140
BstAFI CTTAAG 1 cut(s) 1024
BstBAI YACGTR 1 cut(s) 1350
BstC8I GCNNGC 2 cut(s) 1366, 1537
BstDEI CTNAG 7 cut(s) 655, 672, 726, 1319, 1904, 1969, 2303
BstDSI CCRYGG 1 cut(s) 1185
BstF5I GGATG 4 cut(s) 489, 986, 2068, 2194
BstFNI CGCG 2 cut(s) 303, 1364
BstHHI GCGC 2 cut(s) 47, 838
BstMAI GTCTC 3 cut(s) 1272, 1500, 2303
BstMWI GCNNNNNNNGC 1 cut(s) 2541
BstNI CCWGG 7 cut(s) 480, 927, 1124, 1397, 1788, 2014, 2104
BstNSI RCATGY 3 cut(s) 1539, 1957, 2170
BstSCI CCNGG 8 cut(s) 478, 637, 925, 1122, 1395, 1786, 2012, 2102
BstSFI CTRYAG 1 cut(s) 1716
BstUI CGCG 2 cut(s) 303, 1364
BstV1I GCAGC 4 cut(s) 717, 872, 894, 1615
BstX2I RGATCY 4 cut(s) 433, 1189, 1779, 2431
BstYI RGATCY 4 cut(s) 433, 1189, 1779, 2431
Bsu15I ATCGAT 1 cut(s) 105
Bsu36I CCTNAGG 1 cut(s) 672
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 3 cut(s) 1410, 1765, 2102
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 1185
BtgZI GCGATG 1 cut(s) 776
BtsCI GGATG 4 cut(s) 489, 986, 2068, 2194
BtsI GCAGTG 3 cut(s) 821, 859, 1143
BtsIMutI CAGTG 6 cut(s) 319, 381, 821, 859, 1143, 2485
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 2 cut(s) 1366, 1537
CfoI GCGC 2 cut(s) 47, 838
Cfr13I GGNCC 3 cut(s) 1182, 1400, 1763
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 2 cut(s) 656, 1721
Csp6I GTAC 6 cut(s) 32, 997, 1351, 1682, 1951, 2423
CspCI CAANNNNNGTGG 2 cut(s) 1273, 1308
CviAII CATG 6 cut(s) 516, 1536, 1918, 1954, 2167, 2313
CviQI GTAC 6 cut(s) 32, 997, 1351, 1682, 1951, 2423
DdeI CTNAG 7 cut(s) 655, 672, 726, 1319, 1904, 1969, 2303
EaeI YGGCCR 2 cut(s) 1408, 2100
Eam1104I CTCTTC 1 cut(s) 2560
EarI CTCTTC 1 cut(s) 2560
Eco130I CCWWGG 1 cut(s) 1105
Eco24I GRGCYC 1 cut(s) 2415
Eco47I GGWCC 2 cut(s) 1182, 1400
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 1242
EcoRI GAATTC 2 cut(s) 706, 766
EcoRII CCWGG 7 cut(s) 478, 925, 1122, 1395, 1786, 2012, 2102
EcoT14I CCWWGG 1 cut(s) 1105
EcoT22I ATGCAT 2 cut(s) 1541, 2185
EcoT38I GRGCYC 1 cut(s) 2415
ErhI CCWWGG 1 cut(s) 1105
FaeI CATG 6 cut(s) 519, 1539, 1921, 1957, 2170, 2316
FalI AAGNNNNNCTT 2 cut(s) 1766, 1798
FaqI GGGAC 4 cut(s) 1413, 1661, 1845, 2331
FatI CATG 6 cut(s) 515, 1535, 1917, 1953, 2166, 2312
FauI CCCGC 2 cut(s) 912, 1039
FauNDI CATATG 3 cut(s) 88, 194, 1001
FbaI TGATCA 1 cut(s) 1164
Fnu4HI GCNGC 4 cut(s) 731, 883, 886, 1604
FokI GGATG 4 cut(s) 496, 993, 2075, 2201
FriOI GRGCYC 1 cut(s) 2415
Fsp4HI GCNGC 4 cut(s) 731, 883, 886, 1604
FspBI CTAG 8 cut(s) 72, 125, 401, 563, 1326, 1709, 2240, 2541
GlaI GCGC 2 cut(s) 46, 837
GluI GCNGC 4 cut(s) 731, 883, 886, 1604
GsuI CTGGAG 2 cut(s) 795, 1809
HaeIII GGCC 3 cut(s) 1410, 1765, 2102
HapII CCGG 3 cut(s) 638, 2174, 2435
HgaI GACGC 2 cut(s) 656, 1721
HhaI GCGC 2 cut(s) 47, 838
Hin1I GRCGYC 1 cut(s) 1140
Hin1II CATG 6 cut(s) 519, 1539, 1921, 1957, 2170, 2316
Hin6I GCGC 2 cut(s) 45, 836
HinP1I GCGC 2 cut(s) 45, 836
HincII GTYRAC 3 cut(s) 339, 426, 510
HindII GTYRAC 3 cut(s) 339, 426, 510
HindIII AAGCTT 3 cut(s) 326, 1742, 1966
HinfI GANTC 9 cut(s) 389, 1058, 1211, 1231, 1265, 1902, 1973, 2009, 2319
HpaII CCGG 3 cut(s) 638, 2174, 2435
HphI GGTGA 6 cut(s) 152, 351, 431, 1048, 1229, 2163
Hpy166II GTNNAC 7 cut(s) 339, 426, 510, 594, 1039, 1185, 1771
Hpy188III TCNNGA 9 cut(s) 569, 671, 1796, 1821, 1830, 2006, 2240, 2323, 2594
Hpy8I GTNNAC 7 cut(s) 339, 426, 510, 594, 1039, 1185, 1771
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 7 cut(s) 284, 565, 566, 1030, 1146, 1987, 2591
HpyCH4III ACNGT 7 cut(s) 223, 314, 376, 993, 2146, 2480, 2548
HpyCH4IV ACGT 3 cut(s) 12, 1140, 1349
HpyF10VI GCNNNNNNNGC 1 cut(s) 2541
HpyF3I CTNAG 7 cut(s) 655, 672, 726, 1319, 1904, 1969, 2303
HpySE526I ACGT 3 cut(s) 12, 1140, 1349
Hsp92I GRCGYC 1 cut(s) 1140
Hsp92II CATG 6 cut(s) 519, 1539, 1921, 1957, 2170, 2316
HspAI GCGC 2 cut(s) 45, 836
Ksp22I TGATCA 1 cut(s) 1164
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 4 cut(s) 717, 872, 894, 1615
LweI GCATC 4 cut(s) 474, 654, 971, 1548
MaeI CTAG 8 cut(s) 72, 125, 401, 563, 1326, 1709, 2240, 2541
MaeII ACGT 3 cut(s) 12, 1140, 1349
MaeIII GTNAC 9 cut(s) 208, 316, 497, 1072, 1141, 1217, 2162, 2169, 2464
MfeI CAATTG 2 cut(s) 282, 1731
MflI RGATCY 4 cut(s) 433, 1189, 1779, 2431
MhlI GDGCHC 3 cut(s) 783, 1325, 2415
MlsI TGGCCA 2 cut(s) 1410, 2102
MluNI TGGCCA 2 cut(s) 1410, 2102
MlyI GAGTC 3 cut(s) 1220, 1896, 2313
MmeI TCCRAC 4 cut(s) 288, 485, 2092, 2322
Mox20I TGGCCA 2 cut(s) 1410, 2102
Mph1103I ATGCAT 2 cut(s) 1541, 2185
MroXI GAANNNNTTC 1 cut(s) 355
MscI TGGCCA 2 cut(s) 1410, 2102
MslI CAYNNNNRTG 1 cut(s) 1034
Msp20I TGGCCA 2 cut(s) 1410, 2102
MspA1I CMGCKG 5 cut(s) 431, 730, 885, 907, 2253
MspCI CTTAAG 1 cut(s) 1024
MspI CCGG 3 cut(s) 638, 2174, 2435
MspR9I CCNGG 8 cut(s) 480, 639, 927, 1124, 1397, 1788, 2014, 2104
MunI CAATTG 2 cut(s) 282, 1731
Mva1269I GAATGC 2 cut(s) 1094, 2269
MvaI CCWGG 7 cut(s) 480, 927, 1124, 1397, 1788, 2014, 2104
MvnI CGCG 2 cut(s) 303, 1364
MwoI GCNNNNNNNGC 1 cut(s) 2541
NciI CCSGG 1 cut(s) 639
NdeI CATATG 3 cut(s) 88, 194, 1001
NlaIII CATG 6 cut(s) 519, 1539, 1921, 1957, 2170, 2316
NlaIV GGNNCC 4 cut(s) 398, 435, 1401, 2155
NmuCI GTSAC 4 cut(s) 208, 1141, 1217, 2169
NsiI ATGCAT 2 cut(s) 1541, 2185
NspI RCATGY 3 cut(s) 1539, 1957, 2170
PaeI GCATGC 1 cut(s) 1539
PaeR7I CTCGAG 1 cut(s) 1242
PciI ACATGT 1 cut(s) 2166
PctI GAATGC 2 cut(s) 1094, 2269
PdmI GAANNNNTTC 1 cut(s) 355
PfeI GAWTC 6 cut(s) 389, 1058, 1231, 1265, 1973, 2009
PfoI TCCNGGA 1 cut(s) 1786
PkrI GCNGC 4 cut(s) 732, 884, 887, 1605
PleI GAGTC 3 cut(s) 1219, 1896, 2313
PpsI GAGTC 3 cut(s) 1219, 1896, 2313
Ppu21I YACGTR 1 cut(s) 1350
PscI ACATGT 1 cut(s) 2166
PshBI ATTAAT 2 cut(s) 861, 1380
PsiI TTATAA 2 cut(s) 1427, 2393
Psp6I CCWGG 7 cut(s) 478, 925, 1122, 1395, 1786, 2012, 2102
PspGI CCWGG 7 cut(s) 478, 925, 1122, 1395, 1786, 2012, 2102
PspN4I GGNNCC 4 cut(s) 398, 435, 1401, 2155
PspPI GGNCC 3 cut(s) 1182, 1400, 1763
PspXI VCTCGAGB 1 cut(s) 1242
PsuI RGATCY 4 cut(s) 433, 1189, 1779, 2431
PvuII CAGCTG 2 cut(s) 730, 885
RsaI GTAC 6 cut(s) 33, 998, 1352, 1683, 1952, 2424
RsaNI GTAC 6 cut(s) 32, 997, 1351, 1682, 1951, 2423
RseI CAYNNNNRTG 1 cut(s) 1034
SatI GCNGC 4 cut(s) 731, 883, 886, 1604
Sau96I GGNCC 3 cut(s) 1182, 1400, 1763
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 3 cut(s) 1220, 1896, 2313
ScrFI CCNGG 8 cut(s) 480, 639, 927, 1124, 1397, 1788, 2014, 2104
SduI GDGCHC 3 cut(s) 783, 1325, 2415
SfaNI GCATC 4 cut(s) 474, 654, 971, 1548
SfcI CTRYAG 1 cut(s) 1716
Sfr274I CTCGAG 1 cut(s) 1242
SinI GGWCC 2 cut(s) 1182, 1400
SlaI CTCGAG 1 cut(s) 1242
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 2 cut(s) 1024, 1242
SmoI CTYRAG 2 cut(s) 1024, 1242
SphI GCATGC 1 cut(s) 1539
SsiI CCGC 6 cut(s) 301, 431, 905, 1046, 1601, 2253
SspI AATATT 1 cut(s) 647
SspMI CTAG 8 cut(s) 72, 125, 401, 563, 1326, 1709, 2240, 2541
StyD4I CCNGG 8 cut(s) 478, 637, 925, 1122, 1395, 1786, 2012, 2102
StyI CCWWGG 1 cut(s) 1105
TaaI ACNGT 7 cut(s) 223, 314, 376, 993, 2146, 2480, 2548
TaiI ACGT 3 cut(s) 15, 1143, 1352
TaqI TCGA 6 cut(s) 105, 322, 1064, 1150, 1243, 1608
TaqII GACCGA 1 cut(s) 549
TatI WGTACW 2 cut(s) 31, 1950
TfiI GAWTC 6 cut(s) 389, 1058, 1231, 1265, 1973, 2009
TscAI CASTG 6 cut(s) 319, 381, 821, 859, 1150, 2485
TseFI GTSAC 4 cut(s) 208, 1141, 1217, 2169
TseI GCWGC 4 cut(s) 730, 882, 885, 1603
Tsp45I GTSAC 4 cut(s) 208, 1141, 1217, 2169
TspGWI ACGGA 2 cut(s) 1202, 2542
TspRI CASTG 6 cut(s) 319, 381, 821, 859, 1150, 2485
Vha464I CTTAAG 1 cut(s) 1024
VpaK11BI GGWCC 2 cut(s) 1182, 1400
VspI ATTAAT 2 cut(s) 861, 1380
XapI RAATTY 6 cut(s) 694, 706, 766, 1385, 2194, 2387
XbaI TCTAGA 1 cut(s) 2239
XceI RCATGY 3 cut(s) 1539, 1957, 2170
XhoI CTCGAG 1 cut(s) 1242
XmnI GAANNNNTTC 1 cut(s) 355
XspI CTAG 8 cut(s) 72, 125, 401, 563, 1326, 1709, 2240, 2541
ZraI GACGTC 1 cut(s) 1141
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 2 cut(s) 1541, 2185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.