Rroxscaffold_2G00089200

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000412
Physical Location & Seq
Forward (+)
11106302 .. 11116600
10299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089200.1

Sequence Viewer

Length: 2346 bp
ATGGAATCTTACATCTTTTTCTTGTATGTAGTACTATTGCTGTGCTCTTATGACAACCTATTTTGTTCTGCTAGAGATGCACTAACATATGACAATCCAGTTATCGATGATGGAAATGAAGCTCTTGTTTCAGCAGGAGGAGAATTTGAACTGGGTTTCTTTACCCCTACTCATACATGGAAAAGATATGTGGGCATATGGTATCATAAGGTCACTCCAAGAACAGTTGTATGGGTTGCCAACAGAGATGACCCTCTACCTGCCAATTCCAATGGAGTTCTTGCAATCAAAGAAGGTAAGCTCAGCGTGTTGGACAGTGTTACTCGAAAGCCTTATTGGTCAACAGACATTGGAAAATCTTCATCTTTTAACAAGACAGTGAAGCTAATGGATTCAGGGAACCTGGTGTTAAGGGATTCTGGTGAGTTGACAGCGGATCCTTTGTGGCAGAGCTTTCAAAATCCAACTGATACATTCATTCCTGGGATGCTAATGGATAGTAACTTGAAGTTGACTTCATGGAGCGACGAAGATGACCGAAGAACTGGGAACTACACCTTCAGACTAGATCAAGGAGAGAAGTCAGGGAACCTGTTTACCATCTTAAATAATTCGTTTCCTTACTGGATAAGTGGAGAGCCAGGTAAATATTTAAGCTCAGACAAGATGCCTGCTGACGTAGCTTACTTGTTATCAAATTTTAGCCAGAATTCTTCTCTTTATACAACTCCGCGACAATTTGATTACAATTATCCAAGATTGGTGATAAATTCTACTGGAGCGCTCAATGGTTTGACCTGGAATGACACAATTAAGCAGTGGGTTGTATTTTTCTCTGAGCCAAAAGATGAATGCAGTGTGATTAATGCTTGTGGAAACTTTGGCAGCTGCAATATTGATAATACCCCGCTGGTGTGCAAATGTTTGCCAGGTTTCAACCCCCAGTTTTTAGAGAACTGGCAATCTGGAGATTTTTCGGGCGGATGCAAAAGACAGTCGTCCATATGTGGCGAGAATGACGCCTTCTTAAGCATAAAGGTGAACAAAGCGGGAAGCCCAGATTCATCGACCAATGTTACCAGTGATACAGAATGCAAGAATAAGTGCATTGGTAATTGCAAGTGCCAGGCTTACTCATCTGATTCACAGAATGGTCGAAGGGATAATCAGAATATCACTTGTTATCAATGGTCCACGGATCTAAAAAATCTTGTAGAAGAGTCTGGTGACAATGGCAAGAACCTCAATGCTCGAGTTCCCCGGCCTGATTTAGAATCAACTACAAGAGACTGCGAGCCTTGTGGCACAACCACCGTTCCTTATCCCCTGAGCACCCGAACAAATTGCGGCGACCCCATGTACTTCAGATTCTGGTGCAATACCTCCAGTATTGAATTTAACATCCCGGGATCAGATACTACCTCCTTTAGAGTTATAAATATCAGCCGAAGTACACGACGATTCATTATCAAATCCAAAAATATGGATTATTGTGATTCTAGAAACAGAGTGCTGAATATTCCCCTCCCGTTTAAGGTAATCAGTCGGTGCAGTTCGGACCTGATGAAGAATATTAATTCCGGCATAAATTCATCCAGCGGTCCTTCTGTAATAGAGATTGGTTGGGAGCCACCCTCGGAAACAACCTGCACTACACTAAAAGACTGCAGGGATTGGCCACATTCTACTTGCAATCCAGGAAGAGATGGACAGAAAAGATGCATTTGCGATACAAATTTTCAATGGAACGGCACTGACTTAAATTGTACTCAAGAGCTATCAACTAAGAGATCAAGGAAAAAATTGTTATTGTCTCTGGTTGTCGTGGCAGTCATAAGTGGGATATTTCTGGCATGCATCATTGGTTTTTGGATATGGAGAAGAAAGATTACTAAGAGACGAGATCAAGTAAGTCGTCCGCAGCTCGATAGTGAAAGACGAGTCAAAGAGTTTATAGACACGAGTGACTTCAATGAAGAAGATGACAAAGGCATAGATGTACCTTTTTTTGATTTGCAAAGCATACTAGACGCTACAGATAATTTCTCAATTGGAAACAAGCTTGGACAAGGGGGGTATGGGCCTGTTTACAAGGGGATCTTTACTGGAGGTCAAGAAATTGCTGTAAAGAGGCTATCAAGAGTTTCAGGACAAGGCTTACAGGAGTTTAAGAATGAGGTGGTGCTAATTGCCAAACTTCAACATCGTAATCTTGTAAGACTCAGAGGATATTGCATGAAAGGAGAAGAAAAGATTTTACTCTATGAGTACATGCCTAACAAAAGCTTAGATTCCTTTATATTTGGTTCGCTTCTAATCCTTGGTTTTACTTTTTTTACCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

781

Amino Acids

87.9

Weight (kDa)

6.52

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 170 1.5e-24 D-mannose binding lectin
S_locus_glycop PF00954 218 - 315 2.4e-20 S-locus glycoprotein domain
PAN_2 PF08276 336 - 403 5.1e-09 PAN-like domain
Pkinase PF00069 683 - 767 3.4e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 684 - 767 1.7e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1436
Acc36I ACCTGC 2 cut(s) 268, 1655
AccII CGCG 1 cut(s) 733
AciI CCGC 9 cut(s) 434, 731, 908, 981, 1049, 1347, 1599, 1919, 2340
AclWI GGATC 5 cut(s) 431, 444, 1206, 1417, 2105
AcoI YGGCCR 1 cut(s) 1676
AcsI RAATTY 7 cut(s) 143, 697, 709, 769, 1394, 1588, 1735
AcuI CTGAAG 2 cut(s) 544, 1348
AcyI GRCGYC 1 cut(s) 1020
AfaI GTAC 6 cut(s) 33, 1361, 1453, 1768, 2001, 2270
AfeI AGCGCT 1 cut(s) 783
AfiI CCNNNNNNNGG 1 cut(s) 1534
AflII CTTAAG 1 cut(s) 1027
AgsI TTSAA 8 cut(s) 149, 458, 508, 937, 1394, 1742, 1972, 2201
AjnI CCWGG 7 cut(s) 402, 481, 640, 797, 928, 1125, 1696
AjuI GAANNNNNNNTTGG 2 cut(s) 319, 351
Alw21I GWGCWC 2 cut(s) 47, 1334
Alw26I GTCTC 3 cut(s) 1281, 1818, 1891
AlwI GGATC 5 cut(s) 431, 444, 1206, 1417, 2105
AlwNI CAGNNNCTG 1 cut(s) 1371
Ama87I CYCGRG 2 cut(s) 1251, 1405
Aor51HI AGCGCT 1 cut(s) 783
AoxI GGCC 3 cut(s) 1262, 1676, 2081
ApeKI GCWGC 3 cut(s) 885, 888, 1921
ApoI RAATTY 7 cut(s) 143, 697, 709, 769, 1394, 1588, 1735
AseI ATTAAT 2 cut(s) 864, 1575
Asp700I GAANNNNTTC 1 cut(s) 358
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 4 cut(s) 1191, 1558, 1601, 2081
AsuC2I CCSGG 3 cut(s) 1261, 1406, 1407
AsuHPI GGTGA 4 cut(s) 434, 775, 1051, 1238
AvaI CYCGRG 2 cut(s) 1251, 1405
AvaII GGWCC 3 cut(s) 1191, 1558, 1601
BalI TGGCCA 1 cut(s) 1678
BamHI GGATCC 1 cut(s) 436
BarI GAAGNNNNNNTAC 2 cut(s) 1873, 1905
BauI CACGAG 1 cut(s) 1960
Bbv12I GWGCWC 2 cut(s) 47, 1334
BbvI GCAGC 3 cut(s) 875, 897, 1933
BccI CCATC 3 cut(s) 104, 608, 1700
BceAI ACGGC 1 cut(s) 1765
BcgI CGANNNNNNTGC 2 cut(s) 1326, 1360
BciT130I CCWGG 7 cut(s) 404, 483, 642, 799, 930, 1127, 1698
BcnI CCSGG 3 cut(s) 1261, 1406, 1407
BcoDI GTCTC 3 cut(s) 1281, 1818, 1891
BfaI CTAG 4 cut(s) 72, 566, 1500, 2027
BfmI CTRYAG 2 cut(s) 1666, 2034
BfoI RGCGCY 1 cut(s) 785
BfrI CTTAAG 1 cut(s) 1027
BfuAI ACCTGC 2 cut(s) 268, 1655
BisI GCNGC 4 cut(s) 886, 889, 1348, 1922
BlpI GCTNAGC 1 cut(s) 302
BlsI GCNGC 4 cut(s) 887, 890, 1349, 1923
BmcAI AGTACT 1 cut(s) 33
Bme18I GGWCC 3 cut(s) 1191, 1558, 1601
BmeT110I CYCGRG 2 cut(s) 1251, 1405
BmgT120I GGNCC 4 cut(s) 1191, 1558, 1601, 2081
BmiI GGNNCC 4 cut(s) 401, 438, 590, 1629
BmrI ACTGGG 3 cut(s) 161, 555, 937
BmsI GCATC 6 cut(s) 67, 477, 657, 974, 1709, 1866
BmuI ACTGGG 3 cut(s) 161, 555, 937
BoxI GACNNNNGTC 1 cut(s) 997
BplI GAGNNNNNCTC 2 cut(s) 1619, 1651
BpmI CTGGAG 4 cut(s) 798, 987, 1369, 2127
Bpu10I CCTNAGC 1 cut(s) 1328
Bpu1102I GCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 1755
BpuMI CCSGG 3 cut(s) 1261, 1406, 1407
Bsa29I ATCGAT 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 1020
BsaJI CCNNGG 6 cut(s) 482, 1194, 1259, 1405, 1635, 2320
Bsc4I CCNNNNNNNGG 1 cut(s) 1534
BseBI CCWGG 7 cut(s) 404, 483, 642, 799, 930, 1127, 1698
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 6 cut(s) 482, 1194, 1259, 1405, 1635, 2320
BseGI GGATG 4 cut(s) 492, 989, 1401, 1592
BseLI CCNNNNNNNGG 1 cut(s) 1534
BseMII CTCAG 5 cut(s) 316, 672, 828, 1319, 2236
BseRI GAGGAG 1 cut(s) 153
BseXI GCAGC 3 cut(s) 875, 897, 1933
BsgI GTGCAG 2 cut(s) 1570, 1633
Bsh1236I CGCG 1 cut(s) 733
BshFI GGCC 3 cut(s) 1264, 1678, 2083
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 47, 1334
BsiHKCI CYCGRG 2 cut(s) 1251, 1405
BsiSI CCGG 3 cut(s) 1261, 1406, 1581
BslI CCNNNNNNNGG 1 cut(s) 1534
BsmAI GTCTC 3 cut(s) 1281, 1818, 1891
BsmBI CGTCTC 1 cut(s) 1891
BsmI GAATGC 2 cut(s) 857, 1097
BsnI GGCC 3 cut(s) 1264, 1678, 2083
BsoBI CYCGRG 2 cut(s) 1251, 1405
Bsp1286I GDGCHC 2 cut(s) 47, 1334
Bsp143I GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
Bsp1720I GCTNAGC 1 cut(s) 302
BspACI CCGC 9 cut(s) 434, 731, 908, 981, 1049, 1347, 1599, 1919, 2340
BspANI GGCC 3 cut(s) 1264, 1678, 2083
BspCNI CTCAG 5 cut(s) 315, 671, 829, 1320, 2235
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 1 cut(s) 733
BspLI GGNNCC 4 cut(s) 401, 438, 590, 1629
BspMAI CTGCAG 1 cut(s) 1670
BspMI ACCTGC 2 cut(s) 268, 1655
BspPI GGATC 5 cut(s) 431, 444, 1206, 1417, 2105
BspTI CTTAAG 1 cut(s) 1027
BssECI CCNNGG 6 cut(s) 482, 1194, 1259, 1405, 1635, 2320
BssMI GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
BssNI GRCGYC 1 cut(s) 1020
BssSI CACGAG 1 cut(s) 1960
BssT1I CCWWGG 1 cut(s) 2320
Bst2BI CACGAG 1 cut(s) 1960
Bst2UI CCWGG 7 cut(s) 404, 483, 642, 799, 930, 1127, 1698
Bst4CI ACNGT 5 cut(s) 226, 317, 379, 996, 1315
Bst6I CTCTTC 2 cut(s) 1212, 1696
BstACI GRCGYC 1 cut(s) 1020
BstAFI CTTAAG 1 cut(s) 1027
BstC8I GCNNGC 3 cut(s) 672, 1295, 1855
BstDEI CTNAG 8 cut(s) 302, 658, 837, 1328, 1785, 1893, 2222, 2287
BstDSI CCRYGG 1 cut(s) 1194
BstF5I GGATG 4 cut(s) 492, 989, 1401, 1592
BstFNI CGCG 1 cut(s) 733
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 7 cut(s) 439, 571, 1201, 1412, 1793, 1906, 2100
BstMAI GTCTC 3 cut(s) 1281, 1818, 1891
BstMBI GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
BstMWI GCNNNNNNNGC 2 cut(s) 77, 680
BstNI CCWGG 7 cut(s) 404, 483, 642, 799, 930, 1127, 1698
BstNSI RCATGY 2 cut(s) 1857, 2275
BstPAI GACNNNNGTC 1 cut(s) 997
BstSFI CTRYAG 2 cut(s) 1666, 2034
BstUI CGCG 1 cut(s) 733
BstV1I GCAGC 3 cut(s) 875, 897, 1933
BstX2I RGATCY 3 cut(s) 436, 1198, 2097
BstXI CCANNNNNNTGG 1 cut(s) 1483
BstYI RGATCY 3 cut(s) 436, 1198, 2097
Bsu15I ATCGAT 1 cut(s) 105
BsuRI GGCC 3 cut(s) 1264, 1678, 2083
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 1 cut(s) 1194
BtsCI GGATG 4 cut(s) 492, 989, 1401, 1592
BtsI GCAGTG 2 cut(s) 824, 862
BtsIMutI CAGTG 6 cut(s) 322, 384, 824, 862, 1087, 1752
BveI ACCTGC 2 cut(s) 268, 1655
Cac8I GCNNGC 3 cut(s) 672, 1295, 1855
CaiI CAGNNNCTG 1 cut(s) 1371
CfoI GCGC 1 cut(s) 784
Cfr13I GGNCC 4 cut(s) 1191, 1558, 1601, 2081
Cfr9I CCCGGG 1 cut(s) 1405
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 2 cut(s) 1028, 2039
CsiI ACCWGGT 1 cut(s) 402
Csp6I GTAC 6 cut(s) 32, 1360, 1452, 1767, 2000, 2269
CviAII CATG 6 cut(s) 177, 519, 1357, 1854, 2236, 2272
CviQI GTAC 6 cut(s) 32, 1360, 1452, 1767, 2000, 2269
DdeI CTNAG 8 cut(s) 302, 658, 837, 1328, 1785, 1893, 2222, 2287
DpnI GATC 7 cut(s) 438, 570, 1200, 1411, 1792, 1905, 2099
DpnII GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
EaeI YGGCCR 1 cut(s) 1676
Eam1104I CTCTTC 2 cut(s) 1212, 1696
EarI CTCTTC 2 cut(s) 1212, 1696
EciI GGCGGA 1 cut(s) 996
Eco130I CCWWGG 1 cut(s) 2320
Eco47I GGWCC 3 cut(s) 1191, 1558, 1601
Eco47III AGCGCT 1 cut(s) 783
Eco57I CTGAAG 2 cut(s) 544, 1348
Eco88I CYCGRG 2 cut(s) 1251, 1405
EcoRI GAATTC 1 cut(s) 709
EcoRII CCWGG 7 cut(s) 402, 481, 640, 797, 928, 1125, 1696
EcoT14I CCWWGG 1 cut(s) 2320
EcoT22I ATGCAT 2 cut(s) 1724, 1859
ErhI CCWWGG 1 cut(s) 2320
Esp3I CGTCTC 1 cut(s) 1891
FaeI CATG 6 cut(s) 180, 522, 1360, 1857, 2239, 2275
FalI AAGNNNNNCTT 2 cut(s) 2084, 2116
FatI CATG 6 cut(s) 176, 518, 1356, 1853, 2235, 2271
FauI CCCGC 2 cut(s) 915, 1042
FauNDI CATATG 3 cut(s) 88, 197, 1004
Fnu4HI GCNGC 4 cut(s) 886, 889, 1348, 1922
FokI GGATG 4 cut(s) 499, 996, 1388, 1579
Fsp4HI GCNGC 4 cut(s) 886, 889, 1348, 1922
FspBI CTAG 4 cut(s) 72, 566, 1500, 2027
GlaI GCGC 1 cut(s) 783
GluI GCNGC 4 cut(s) 886, 889, 1348, 1922
GsuI CTGGAG 4 cut(s) 798, 987, 1369, 2127
HaeII RGCGCY 1 cut(s) 785
HaeIII GGCC 3 cut(s) 1264, 1678, 2083
HapII CCGG 3 cut(s) 1261, 1406, 1581
HgaI GACGC 2 cut(s) 1028, 2039
HhaI GCGC 1 cut(s) 784
Hin1I GRCGYC 1 cut(s) 1020
Hin1II CATG 6 cut(s) 180, 522, 1360, 1857, 2239, 2275
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 3 cut(s) 342, 429, 513
HindII GTYRAC 3 cut(s) 342, 429, 513
HindIII AAGCTT 2 cut(s) 2060, 2284
HpaII CCGG 3 cut(s) 1261, 1406, 1581
HphI GGTGA 4 cut(s) 434, 775, 1051, 1238
Hpy166II GTNNAC 8 cut(s) 342, 429, 513, 597, 1042, 1194, 1454, 2089
Hpy188III TCNNGA 6 cut(s) 966, 1500, 1772, 2114, 2139, 2148
Hpy8I GTNNAC 8 cut(s) 342, 429, 513, 597, 1042, 1194, 1454, 2089
Hpy99I CGWCG 2 cut(s) 530, 1461
HpyAV CCTTC 5 cut(s) 287, 568, 1033, 1152, 1614
HpyCH4III ACNGT 5 cut(s) 226, 317, 379, 996, 1315
HpyCH4IV ACGT 1 cut(s) 678
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 680
HpyF3I CTNAG 8 cut(s) 302, 658, 837, 1328, 1785, 1893, 2222, 2287
HpySE526I ACGT 1 cut(s) 678
Hsp92I GRCGYC 1 cut(s) 1020
Hsp92II CATG 6 cut(s) 180, 522, 1360, 1857, 2239, 2275
HspAI GCGC 1 cut(s) 782
Kzo9I GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
LmnI GCTCC 3 cut(s) 522, 779, 1627
Lsp1109I GCAGC 3 cut(s) 875, 897, 1933
LweI GCATC 6 cut(s) 67, 477, 657, 974, 1709, 1866
MabI ACCWGGT 1 cut(s) 402
MaeI CTAG 4 cut(s) 72, 566, 1500, 2027
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 6 cut(s) 211, 319, 500, 1075, 1226, 1964
MalI GATC 7 cut(s) 438, 570, 1200, 1411, 1792, 1905, 2099
MboI GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
MfeI CAATTG 1 cut(s) 2049
MflI RGATCY 3 cut(s) 436, 1198, 2097
MhlI GDGCHC 2 cut(s) 47, 1334
MlsI TGGCCA 1 cut(s) 1678
MluNI TGGCCA 1 cut(s) 1678
MlyI GAGTC 3 cut(s) 1229, 1950, 2214
MmeI TCCRAC 2 cut(s) 291, 488
Mox20I TGGCCA 1 cut(s) 1678
Mph1103I ATGCAT 2 cut(s) 1724, 1859
MroXI GAANNNNTTC 1 cut(s) 358
MscI TGGCCA 1 cut(s) 1678
MslI CAYNNNNRTG 1 cut(s) 1037
Msp20I TGGCCA 1 cut(s) 1678
MspA1I CMGCKG 4 cut(s) 434, 888, 910, 1599
MspCI CTTAAG 1 cut(s) 1027
MspI CCGG 3 cut(s) 1261, 1406, 1581
MunI CAATTG 1 cut(s) 2049
Mva1269I GAATGC 2 cut(s) 857, 1097
MvaI CCWGG 7 cut(s) 404, 483, 642, 799, 930, 1127, 1698
MvnI CGCG 1 cut(s) 733
MwoI GCNNNNNNNGC 2 cut(s) 77, 680
NciI CCSGG 3 cut(s) 1261, 1406, 1407
NdeI CATATG 3 cut(s) 88, 197, 1004
NdeII GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
NlaIII CATG 6 cut(s) 180, 522, 1360, 1857, 2239, 2275
NlaIV GGNNCC 4 cut(s) 401, 438, 590, 1629
NmuCI GTSAC 3 cut(s) 211, 1226, 1964
NsiI ATGCAT 2 cut(s) 1724, 1859
NspI RCATGY 2 cut(s) 1857, 2275
PaeI GCATGC 1 cut(s) 1857
PaeR7I CTCGAG 1 cut(s) 1251
PctI GAATGC 2 cut(s) 857, 1097
PdmI GAANNNNTTC 1 cut(s) 358
PfoI TCCNGGA 1 cut(s) 1696
PkrI GCNGC 4 cut(s) 887, 890, 1349, 1923
PleI GAGTC 3 cut(s) 1228, 1949, 2214
PpsI GAGTC 3 cut(s) 1228, 1949, 2214
PshAI GACNNNNGTC 1 cut(s) 997
PshBI ATTAAT 2 cut(s) 864, 1575
PsiI TTATAA 1 cut(s) 1436
Psp6I CCWGG 7 cut(s) 402, 481, 640, 797, 928, 1125, 1696
PspGI CCWGG 7 cut(s) 402, 481, 640, 797, 928, 1125, 1696
PspN4I GGNNCC 4 cut(s) 401, 438, 590, 1629
PspPI GGNCC 4 cut(s) 1191, 1558, 1601, 2081
PspXI VCTCGAGB 1 cut(s) 1251
PstI CTGCAG 1 cut(s) 1670
PstNI CAGNNNCTG 1 cut(s) 1371
PsuI RGATCY 3 cut(s) 436, 1198, 2097
PvuII CAGCTG 1 cut(s) 888
RsaI GTAC 6 cut(s) 33, 1361, 1453, 1768, 2001, 2270
RsaNI GTAC 6 cut(s) 32, 1360, 1452, 1767, 2000, 2269
RseI CAYNNNNRTG 1 cut(s) 1037
SatI GCNGC 4 cut(s) 886, 889, 1348, 1922
Sau3AI GATC 7 cut(s) 436, 568, 1198, 1409, 1790, 1903, 2097
Sau96I GGNCC 4 cut(s) 1191, 1558, 1601, 2081
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 3 cut(s) 1229, 1950, 2214
SduI GDGCHC 2 cut(s) 47, 1334
SexAI ACCWGGT 1 cut(s) 402
SfaNI GCATC 6 cut(s) 67, 477, 657, 974, 1709, 1866
SfcI CTRYAG 2 cut(s) 1666, 2034
Sfr274I CTCGAG 1 cut(s) 1251
SinI GGWCC 3 cut(s) 1191, 1558, 1601
SlaI CTCGAG 1 cut(s) 1251
SmaI CCCGGG 1 cut(s) 1407
SmiMI CAYNNNNRTG 1 cut(s) 1037
SmlI CTYRAG 3 cut(s) 1027, 1251, 1770
SmoI CTYRAG 3 cut(s) 1027, 1251, 1770
SphI GCATGC 1 cut(s) 1857
SsiI CCGC 9 cut(s) 434, 731, 908, 981, 1049, 1347, 1599, 1919, 2340
SspI AATATT 4 cut(s) 650, 895, 1519, 1573
SspMI CTAG 4 cut(s) 72, 566, 1500, 2027
StyI CCWWGG 1 cut(s) 2320
TaaI ACNGT 5 cut(s) 226, 317, 379, 996, 1315
TaiI ACGT 1 cut(s) 681
TaqI TCGA 6 cut(s) 105, 325, 1067, 1156, 1252, 1926
TaqII GACCGA 1 cut(s) 552
TatI WGTACW 5 cut(s) 31, 1359, 1451, 1766, 2268
TauI GCSGC 1 cut(s) 1350
TscAI CASTG 6 cut(s) 322, 384, 824, 862, 1087, 1759
TseFI GTSAC 3 cut(s) 211, 1226, 1964
TseI GCWGC 3 cut(s) 885, 888, 1921
Tsp45I GTSAC 3 cut(s) 211, 1226, 1964
TspGWI ACGGA 1 cut(s) 1211
TspMI CCCGGG 1 cut(s) 1405
TspRI CASTG 6 cut(s) 322, 384, 824, 862, 1087, 1759
Vha464I CTTAAG 1 cut(s) 1027
VpaK11BI GGWCC 3 cut(s) 1191, 1558, 1601
VspI ATTAAT 2 cut(s) 864, 1575
XapI RAATTY 7 cut(s) 143, 697, 709, 769, 1394, 1588, 1735
XbaI TCTAGA 1 cut(s) 1499
XceI RCATGY 2 cut(s) 1857, 2275
XhoI CTCGAG 1 cut(s) 1251
XmaI CCCGGG 1 cut(s) 1405
XmnI GAANNNNTTC 1 cut(s) 358
XspI CTAG 4 cut(s) 72, 566, 1500, 2027
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 2 cut(s) 1724, 1859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.