serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 382 |
| AccI | GTMKAC | 2 cut(s) | 1212, 1352 |
| AccII | CGCG | 1 cut(s) | 1766 |
| AciI | CCGC | 7 cut(s) | 618, 702, 860, 1002, 1037, 1472, 1766 |
| AclWI | GGATC | 3 cut(s) | 1083, 2057, 2453 |
| AcsI | RAATTY | 7 cut(s) | 510, 622, 772, 799, 952, 1090, 1808 |
| AcuI | CTGAAG | 1 cut(s) | 616 |
| AfaI | GTAC | 7 cut(s) | 343, 692, 1127, 1435, 1644, 1913, 2451 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 382, 617, 1199, 2046, 2092 |
| AflII | CTTAAG | 1 cut(s) | 1865 |
| AflIII | ACRYGT | 2 cut(s) | 1236, 2193 |
| AhlI | ACTAGT | 1 cut(s) | 338 |
| AjnI | CCWGG | 3 cut(s) | 553, 1057, 1071 |
| AluBI | AGCT | 8 cut(s) | 525, 732, 1017, 1167, 1705, 1974, 2250, 2576 |
| AluI | AGCT | 8 cut(s) | 525, 732, 1017, 1167, 1705, 1974, 2250, 2576 |
| Alw26I | GTCTC | 4 cut(s) | 1092, 1461, 2202, 2338 |
| AlwI | GGATC | 3 cut(s) | 1083, 2057, 2453 |
| Ama87I | CYCGRG | 2 cut(s) | 610, 2010 |
| AoxI | GGCC | 4 cut(s) | 1034, 1363, 1724, 1753 |
| ApeKI | GCWGC | 3 cut(s) | 1014, 1017, 1039 |
| ApoI | RAATTY | 7 cut(s) | 510, 622, 772, 799, 952, 1090, 1808 |
| AseI | ATTAAT | 2 cut(s) | 897, 933 |
| AsiSI | GCGATCGC | 1 cut(s) | 2470 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 684 |
| AspLEI | GCGC | 2 cut(s) | 970, 1300 |
| AspS9I | GGNCC | 2 cut(s) | 1363, 1724 |
| AsuC2I | CCSGG | 3 cut(s) | 466, 611, 612 |
| AsuHPI | GGTGA | 6 cut(s) | 110, 228, 266, 904, 1780, 2485 |
| AsuII | TTCGAA | 1 cut(s) | 796 |
| AvaI | CYCGRG | 2 cut(s) | 610, 2010 |
| BbsI | GAAGAC | 2 cut(s) | 1434, 1573 |
| BbvI | GCAGC | 3 cut(s) | 1004, 1026, 1026 |
| BccI | CCATC | 5 cut(s) | 83, 359, 1352, 1426, 1459 |
| BceAI | ACGGC | 2 cut(s) | 169, 432 |
| BciT130I | CCWGG | 3 cut(s) | 555, 1059, 1073 |
| BciVI | GTATCC | 2 cut(s) | 379, 1712 |
| BclI | TGATCA | 3 cut(s) | 493, 736, 2490 |
| BcnI | CCSGG | 3 cut(s) | 466, 611, 612 |
| BcoDI | GTCTC | 4 cut(s) | 1092, 1461, 2202, 2338 |
| BcuI | ACTAGT | 1 cut(s) | 338 |
| BfaI | CTAG | 5 cut(s) | 135, 339, 1670, 2087, 2586 |
| BfmI | CTRYAG | 4 cut(s) | 750, 1079, 1677, 2172 |
| BfrI | CTTAAG | 1 cut(s) | 1865 |
| BfuI | GTATCC | 2 cut(s) | 379, 1712 |
| BglII | AGATCT | 1 cut(s) | 1896 |
| BisI | GCNGC | 5 cut(s) | 1003, 1015, 1018, 1037, 1040 |
| BlsI | GCNGC | 5 cut(s) | 1004, 1016, 1019, 1038, 1041 |
| BmcAI | AGTACT | 1 cut(s) | 343 |
| Bme1390I | CCNGG | 6 cut(s) | 466, 555, 611, 612, 1059, 1073 |
| BmeT110I | CYCGRG | 2 cut(s) | 610, 2010 |
| BmgT120I | GGNCC | 2 cut(s) | 1363, 1724 |
| BmiI | GGNNCC | 3 cut(s) | 470, 1364, 2529 |
| BmrFI | CCNGG | 6 cut(s) | 466, 555, 611, 612, 1059, 1073 |
| BmsI | GCATC | 7 cut(s) | 56, 1103, 1247, 1285, 1512, 1971, 2001 |
| BpiI | GAAGAC | 2 cut(s) | 1434, 1573 |
| BpmI | CTGGAG | 4 cut(s) | 222, 1116, 2184, 2551 |
| Bpu14I | TTCGAA | 1 cut(s) | 796 |
| BpuEI | CTTGAG | 2 cut(s) | 1183, 2288 |
| BpuMI | CCSGG | 3 cut(s) | 466, 611, 612 |
| BsaJI | CCNNGG | 4 cut(s) | 287, 554, 610, 1071 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 382, 617, 1199, 2046, 2092 |
| Bse1I | ACTGG | 6 cut(s) | 349, 410, 658, 698, 910, 2413 |
| Bse3DI | GCAATG | 1 cut(s) | 2523 |
| BseBI | CCWGG | 3 cut(s) | 555, 1059, 1073 |
| BseDI | CCNNGG | 4 cut(s) | 287, 554, 610, 1071 |
| BseGI | GGATG | 4 cut(s) | 564, 1118, 1300, 1503 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 382, 617, 1199, 2046, 2092 |
| BseMI | GCAATG | 1 cut(s) | 2523 |
| BseMII | CTCAG | 3 cut(s) | 1984, 2168, 2241 |
| BseNI | ACTGG | 6 cut(s) | 349, 410, 658, 698, 910, 2413 |
| BseRI | GAGGAG | 1 cut(s) | 2108 |
| BseXI | GCAGC | 3 cut(s) | 1004, 1026, 1026 |
| BseYI | CCCAGC | 1 cut(s) | 1974 |
| BsgI | GTGCAG | 1 cut(s) | 1333 |
| Bsh1236I | CGCG | 1 cut(s) | 1766 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2470 |
| BshFI | GGCC | 4 cut(s) | 1036, 1365, 1726, 1755 |
| BsiEI | CGRYCG | 1 cut(s) | 2470 |
| BsiHKCI | CYCGRG | 2 cut(s) | 610, 2010 |
| BsiSI | CCGG | 3 cut(s) | 465, 611, 2525 |
| BsiWI | CGTACG | 1 cut(s) | 1433 |
| BslFI | GGGAC | 2 cut(s) | 259, 1337 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 382, 617, 1199, 2046, 2092 |
| BsmAI | GTCTC | 4 cut(s) | 1092, 1461, 2202, 2338 |
| BsmFI | GGGAC | 2 cut(s) | 259, 1337 |
| BsmI | GAATGC | 1 cut(s) | 1232 |
| BsnI | GGCC | 4 cut(s) | 1036, 1365, 1726, 1755 |
| BsoBI | CYCGRG | 2 cut(s) | 610, 2010 |
| Bsp119I | TTCGAA | 1 cut(s) | 796 |
| BspACI | CCGC | 7 cut(s) | 618, 702, 860, 1002, 1037, 1472, 1766 |
| BspANI | GGCC | 4 cut(s) | 1036, 1365, 1726, 1755 |
| BspCNI | CTCAG | 3 cut(s) | 1983, 2167, 2240 |
| BspFNI | CGCG | 1 cut(s) | 1766 |
| BspLI | GGNNCC | 3 cut(s) | 470, 1364, 2529 |
| BspMAI | CTGCAG | 1 cut(s) | 754 |
| BspPI | GGATC | 3 cut(s) | 1083, 2057, 2453 |
| BspT104I | TTCGAA | 1 cut(s) | 796 |
| BspTI | CTTAAG | 1 cut(s) | 1865 |
| BsrDI | GCAATG | 1 cut(s) | 2523 |
| BsrI | ACTGG | 6 cut(s) | 349, 410, 658, 698, 910, 2413 |
| BssECI | CCNNGG | 4 cut(s) | 287, 554, 610, 1071 |
| BssNAI | GTATAC | 1 cut(s) | 1353 |
| Bst1107I | GTATAC | 1 cut(s) | 1353 |
| Bst2UI | CCWGG | 3 cut(s) | 555, 1059, 1073 |
| Bst4CI | ACNGT | 6 cut(s) | 173, 295, 918, 1433, 2173, 2507 |
| Bst6I | CTCTTC | 3 cut(s) | 841, 1341, 1620 |
| BstAFI | CTTAAG | 1 cut(s) | 1865 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 53 |
| BstBI | TTCGAA | 1 cut(s) | 796 |
| BstC8I | GCNNGC | 5 cut(s) | 65, 1000, 1501, 1802, 2541 |
| BstDEI | CTNAG | 6 cut(s) | 281, 479, 1559, 1970, 2154, 2227 |
| BstEII | GGTNACC | 1 cut(s) | 368 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2090 |
| BstF5I | GGATG | 4 cut(s) | 564, 1118, 1300, 1503 |
| BstFNI | CGCG | 1 cut(s) | 1766 |
| BstHHI | GCGC | 2 cut(s) | 970, 1300 |
| BstMAI | GTCTC | 4 cut(s) | 1092, 1461, 2202, 2338 |
| BstMCI | CGRYCG | 1 cut(s) | 2470 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 53, 1011, 2409, 2568 |
| BstNI | CCWGG | 3 cut(s) | 555, 1059, 1073 |
| BstNSI | RCATGY | 4 cut(s) | 67, 1918, 1966, 2197 |
| BstPI | GGTNACC | 1 cut(s) | 368 |
| BstSCI | CCNGG | 6 cut(s) | 464, 553, 609, 610, 1057, 1071 |
| BstSFI | CTRYAG | 4 cut(s) | 750, 1079, 1677, 2172 |
| BstUI | CGCG | 1 cut(s) | 1766 |
| BstV1I | GCAGC | 3 cut(s) | 1004, 1026, 1026 |
| BstV2I | GAAGAC | 2 cut(s) | 1434, 1573 |
| BstX2I | RGATCY | 3 cut(s) | 1075, 1896, 2458 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2623 |
| BstYI | RGATCY | 3 cut(s) | 1075, 1896, 2458 |
| BstZ17I | GTATAC | 1 cut(s) | 1353 |
| BsuI | GTATCC | 2 cut(s) | 379, 1712 |
| BsuRI | GGCC | 4 cut(s) | 1036, 1365, 1726, 1755 |
| BtgZI | GCGATG | 1 cut(s) | 504 |
| BtsCI | GGATG | 4 cut(s) | 564, 1118, 1300, 1503 |
| BtsI | GCAGTG | 5 cut(s) | 99, 490, 510, 953, 1091 |
| Cac8I | GCNNGC | 5 cut(s) | 65, 1000, 1501, 1802, 2541 |
| CfoI | GCGC | 2 cut(s) | 970, 1300 |
| Cfr13I | GGNCC | 2 cut(s) | 1363, 1724 |
| Cfr9I | CCCGGG | 1 cut(s) | 610 |
| CseI | GACGC | 2 cut(s) | 460, 1682 |
| Csp6I | GTAC | 7 cut(s) | 342, 691, 1126, 1434, 1643, 1912, 2450 |
| CviQI | GTAC | 7 cut(s) | 342, 691, 1126, 1434, 1643, 1912, 2450 |
| DdeI | CTNAG | 6 cut(s) | 281, 479, 1559, 1970, 2154, 2227 |
| Eam1104I | CTCTTC | 3 cut(s) | 841, 1341, 1620 |
| EarI | CTCTTC | 3 cut(s) | 841, 1341, 1620 |
| EciI | GGCGGA | 1 cut(s) | 717 |
| Eco57I | CTGAAG | 1 cut(s) | 616 |
| Eco88I | CYCGRG | 2 cut(s) | 610, 2010 |
| Eco91I | GGTNACC | 1 cut(s) | 368 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2090 |
| EcoO65I | GGTNACC | 1 cut(s) | 368 |
| EcoRI | GAATTC | 1 cut(s) | 1090 |
| EcoRII | CCWGG | 3 cut(s) | 553, 1057, 1071 |
| EcoT22I | ATGCAT | 2 cut(s) | 2212, 2341 |
| FaqI | GGGAC | 2 cut(s) | 259, 1337 |
| FauI | CCCGC | 1 cut(s) | 611 |
| FauNDI | CATATG | 2 cut(s) | 266, 1273 |
| FbaI | TGATCA | 3 cut(s) | 493, 736, 2490 |
| FblI | GTMKAC | 2 cut(s) | 1212, 1352 |
| Fnu4HI | GCNGC | 5 cut(s) | 1003, 1015, 1018, 1037, 1040 |
| FokI | GGATG | 4 cut(s) | 571, 1125, 1307, 1490 |
| Fsp4HI | GCNGC | 5 cut(s) | 1003, 1015, 1018, 1037, 1040 |
| FspBI | CTAG | 5 cut(s) | 135, 339, 1670, 2087, 2586 |
| GlaI | GCGC | 2 cut(s) | 969, 1299 |
| GluI | GCNGC | 5 cut(s) | 1003, 1015, 1018, 1037, 1040 |
| GsaI | CCCAGC | 1 cut(s) | 1978 |
| GsuI | CTGGAG | 4 cut(s) | 222, 1116, 2184, 2551 |
| HaeIII | GGCC | 4 cut(s) | 1036, 1365, 1726, 1755 |
| HapII | CCGG | 3 cut(s) | 465, 611, 2525 |
| HgaI | GACGC | 2 cut(s) | 460, 1682 |
| HhaI | GCGC | 2 cut(s) | 970, 1300 |
| Hin6I | GCGC | 2 cut(s) | 968, 1298 |
| HinP1I | GCGC | 2 cut(s) | 968, 1298 |
| HincII | GTYRAC | 4 cut(s) | 24, 411, 585, 1213 |
| HindII | GTYRAC | 4 cut(s) | 24, 411, 585, 1213 |
| HindIII | AAGCTT | 1 cut(s) | 1703 |
| HpaI | GTTAAC | 1 cut(s) | 24 |
| HpaII | CCGG | 3 cut(s) | 465, 611, 2525 |
| HphI | GGTGA | 6 cut(s) | 110, 228, 266, 904, 1780, 2485 |
| Hpy166II | GTNNAC | 6 cut(s) | 24, 411, 585, 1213, 1353, 1732 |
| Hpy8I | GTNNAC | 6 cut(s) | 24, 411, 585, 1213, 1353, 1732 |
| Hpy99I | CGWCG | 3 cut(s) | 44, 173, 454 |
| HpyAV | CCTTC | 5 cut(s) | 243, 1168, 1284, 1625, 2624 |
| HpyCH4III | ACNGT | 6 cut(s) | 173, 295, 918, 1433, 2173, 2507 |
| HpyCH4IV | ACGT | 2 cut(s) | 39, 1236 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 53, 1011, 2409, 2568 |
| HpyF3I | CTNAG | 6 cut(s) | 281, 479, 1559, 1970, 2154, 2227 |
| HpySE526I | ACGT | 2 cut(s) | 39, 1236 |
| HspAI | GCGC | 2 cut(s) | 968, 1298 |
| Ksp22I | TGATCA | 3 cut(s) | 493, 736, 2490 |
| KspAI | GTTAAC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 2533 |
| Lsp1109I | GCAGC | 3 cut(s) | 1004, 1026, 1026 |
| LweI | GCATC | 7 cut(s) | 56, 1103, 1247, 1285, 1512, 1971, 2001 |
| MaeI | CTAG | 5 cut(s) | 135, 339, 1670, 2087, 2586 |
| MaeII | ACGT | 2 cut(s) | 39, 1236 |
| MaeIII | GTNAC | 6 cut(s) | 368, 401, 754, 1139, 1330, 2189 |
| MflI | RGATCY | 3 cut(s) | 1075, 1896, 2458 |
| MlyI | GAGTC | 5 cut(s) | 835, 1599, 2340, 2380, 2433 |
| MmeI | TCCRAC | 5 cut(s) | 363, 560, 2119, 2158, 2416 |
| Mph1103I | ATGCAT | 2 cut(s) | 2212, 2341 |
| MroXI | GAANNNNTTC | 1 cut(s) | 684 |
| MseI | TTAA | 9 cut(s) | 23, 437, 897, 933, 993, 1244, 1481, 1866, 2375 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1149 |
| MspA1I | CMGCKG | 3 cut(s) | 1017, 1039, 1974 |
| MspCI | CTTAAG | 1 cut(s) | 1865 |
| MspI | CCGG | 3 cut(s) | 465, 611, 2525 |
| MspR9I | CCNGG | 6 cut(s) | 466, 555, 611, 612, 1059, 1073 |
| Mva1269I | GAATGC | 1 cut(s) | 1232 |
| MvaI | CCWGG | 3 cut(s) | 555, 1059, 1073 |
| MvnI | CGCG | 1 cut(s) | 1766 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 53, 1011, 2409, 2568 |
| NciI | CCSGG | 3 cut(s) | 466, 611, 612 |
| NdeI | CATATG | 2 cut(s) | 266, 1273 |
| NlaIV | GGNNCC | 3 cut(s) | 470, 1364, 2529 |
| NmuCI | GTSAC | 1 cut(s) | 1139 |
| NsiI | ATGCAT | 2 cut(s) | 2212, 2341 |
| NspI | RCATGY | 4 cut(s) | 67, 1918, 1966, 2197 |
| NspV | TTCGAA | 1 cut(s) | 796 |
| PaeI | GCATGC | 1 cut(s) | 67 |
| PaeR7I | CTCGAG | 1 cut(s) | 2010 |
| PciI | ACATGT | 1 cut(s) | 2193 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 446 |
| PctI | GAATGC | 1 cut(s) | 1232 |
| PdmI | GAANNNNTTC | 1 cut(s) | 684 |
| PfeI | GAWTC | 7 cut(s) | 179, 461, 685, 1196, 1203, 2004, 2036 |
| Pfl23II | CGTACG | 1 cut(s) | 1433 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 382 |
| PfoI | TCCNGGA | 1 cut(s) | 464 |
| PkrI | GCNGC | 5 cut(s) | 1004, 1016, 1019, 1038, 1041 |
| Ple19I | CGATCG | 1 cut(s) | 2470 |
| PleI | GAGTC | 5 cut(s) | 834, 1598, 2340, 2380, 2433 |
| PpsI | GAGTC | 5 cut(s) | 834, 1598, 2340, 2380, 2433 |
| PscI | ACATGT | 1 cut(s) | 2193 |
| PshBI | ATTAAT | 2 cut(s) | 897, 933 |
| Psp6I | CCWGG | 3 cut(s) | 553, 1057, 1071 |
| PspEI | GGTNACC | 1 cut(s) | 368 |
| PspFI | CCCAGC | 1 cut(s) | 1974 |
| PspGI | CCWGG | 3 cut(s) | 553, 1057, 1071 |
| PspLI | CGTACG | 1 cut(s) | 1433 |
| PspN4I | GGNNCC | 3 cut(s) | 470, 1364, 2529 |
| PspPI | GGNCC | 2 cut(s) | 1363, 1724 |
| PspXI | VCTCGAGB | 1 cut(s) | 2010 |
| PstI | CTGCAG | 1 cut(s) | 754 |
| PsuI | RGATCY | 3 cut(s) | 1075, 1896, 2458 |
| PvuI | CGATCG | 1 cut(s) | 2470 |
| PvuII | CAGCTG | 2 cut(s) | 1017, 1974 |
| RgaI | GCGATCGC | 1 cut(s) | 2470 |
| RsaI | GTAC | 7 cut(s) | 343, 692, 1127, 1435, 1644, 1913, 2451 |
| RsaNI | GTAC | 7 cut(s) | 342, 691, 1126, 1434, 1643, 1912, 2450 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1149 |
| SalI | GTCGAC | 1 cut(s) | 1211 |
| SaqAI | TTAA | 9 cut(s) | 23, 437, 897, 933, 993, 1244, 1481, 1866, 2375 |
| SatI | GCNGC | 5 cut(s) | 1003, 1015, 1018, 1037, 1040 |
| Sau96I | GGNCC | 2 cut(s) | 1363, 1724 |
| ScaI | AGTACT | 1 cut(s) | 343 |
| SchI | GAGTC | 5 cut(s) | 835, 1599, 2340, 2380, 2433 |
| ScrFI | CCNGG | 6 cut(s) | 466, 555, 611, 612, 1059, 1073 |
| SfaAI | GCGATCGC | 1 cut(s) | 2470 |
| SfaNI | GCATC | 7 cut(s) | 56, 1103, 1247, 1285, 1512, 1971, 2001 |
| SfcI | CTRYAG | 4 cut(s) | 750, 1079, 1677, 2172 |
| Sfr274I | CTCGAG | 1 cut(s) | 2010 |
| SfuI | TTCGAA | 1 cut(s) | 796 |
| SgfI | GCGATCGC | 1 cut(s) | 2470 |
| SlaI | CTCGAG | 1 cut(s) | 2010 |
| SmaI | CCCGGG | 1 cut(s) | 612 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1149 |
| SmlI | CTYRAG | 4 cut(s) | 1162, 1865, 2010, 2267 |
| SmoI | CTYRAG | 4 cut(s) | 1162, 1865, 2010, 2267 |
| SpeI | ACTAGT | 1 cut(s) | 338 |
| SphI | GCATGC | 1 cut(s) | 67 |
| SsiI | CCGC | 7 cut(s) | 618, 702, 860, 1002, 1037, 1472, 1766 |
| SspI | AATATT | 3 cut(s) | 811, 901, 2080 |
| SspMI | CTAG | 5 cut(s) | 135, 339, 1670, 2087, 2586 |
| StyD4I | CCNGG | 6 cut(s) | 464, 553, 609, 610, 1057, 1071 |
| TaaI | ACNGT | 6 cut(s) | 173, 295, 918, 1433, 2173, 2507 |
| TaiI | ACGT | 2 cut(s) | 42, 1239 |
| TaqI | TCGA | 7 cut(s) | 796, 1212, 1947, 2011, 2309, 2483, 2502 |
| TatI | WGTACW | 3 cut(s) | 341, 690, 1911 |
| TauI | GCSGC | 2 cut(s) | 1005, 1039 |
| TfiI | GAWTC | 7 cut(s) | 179, 461, 685, 1196, 1203, 2004, 2036 |
| Tru1I | TTAA | 9 cut(s) | 23, 437, 897, 933, 993, 1244, 1481, 1866, 2375 |
| Tru9I | TTAA | 9 cut(s) | 23, 437, 897, 933, 993, 1244, 1481, 1866, 2375 |
| TseFI | GTSAC | 1 cut(s) | 1139 |
| TseI | GCWGC | 3 cut(s) | 1014, 1017, 1039 |
| Tsp45I | GTSAC | 1 cut(s) | 1139 |
| TspGWI | ACGGA | 1 cut(s) | 2232 |
| TspMI | CCCGGG | 1 cut(s) | 610 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 382 |
| Vha464I | CTTAAG | 1 cut(s) | 1865 |
| VspI | ATTAAT | 2 cut(s) | 897, 933 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2090 |
| XapI | RAATTY | 7 cut(s) | 510, 622, 772, 799, 952, 1090, 1808 |
| XceI | RCATGY | 4 cut(s) | 67, 1918, 1966, 2197 |
| XhoI | CTCGAG | 1 cut(s) | 2010 |
| XmaI | CCCGGG | 1 cut(s) | 610 |
| XmiI | GTMKAC | 2 cut(s) | 1212, 1352 |
| XmnI | GAANNNNTTC | 1 cut(s) | 684 |
| XspI | CTAG | 5 cut(s) | 135, 339, 1670, 2087, 2586 |
| ZrmI | AGTACT | 1 cut(s) | 343 |
| Zsp2I | ATGCAT | 2 cut(s) | 2212, 2341 |