MD09G1099900.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000412
Physical Location & Seq
Forward (+)
7299549 .. 7302085
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1099900.v1.1.491

Sequence Viewer

Length: 1959 bp
ATGATGTTTCTCAGAGGGAGGTTAACAAGATCAAATTACGTCGTTACATGGTTTGCGAATCGCATGCTGTTTTCCATCTTCGTTTCTTATGCAGTAGCGGTCTTGTTGTGCTCATCTCACCCATTTTGCTGTGCTAGAGATACAGTAACATACGACACTCCAATTACCGACGGTGTTGAATCAGAAACTCTTGTTTCGTCTGGTGGAAGATTTGAACTCGGATTCTTTAGCCCTTCACCTGAAGGGACTCAAAGATATGTTGGCATATGGTATCACCAACCGAGTCCGAGGACAGTGGTATGGGTTGCCAACAGAGAAAAGCCAGTTGACAATTCCACTGGTAATGGAAGTCTTGCACTCCAAGATGGTAACCTCCATGTGTTGGATGCTGCTGGAAAGAGGTACTGGTCAACGGAGGTTGAAACATCCGAGTCTTTGACGCAGACTGTGACGCTCATGGATTCCGGGAACCTTGTTCTAAGCAGTGGTCATGATAAGTTGGCAGTGAATATTCTGTGGCAGAGCTTTCAAAGTCCAACCGATACATTCATTCCTGGGATGTTAATGGATAAAAGCTTGGTGTTGACTTCATGGAGAGGCGAAGATGACCCGAAAAGCGGGCAATTCATCTTCAAAAAAGATCCAGTTGGAGAGGACCAGTATGTCATCACAAAATCAAAATCAGATTTAGAATCGATTCTGTACTGGAAAGGCGGAGAACGGGCAACTGATGCATTTTACAGCTTTGATCAAATGCTTCCTGCGGTTACTTACTTACTATCAAATTTCAGCAAGAAATATGTTGAAAGAAATTACCCCAAGATTTTCGGTTCTCCGAGTCAAAACTCTTATAAAAATATAGCAATTCCGCCACAATCAGAATACAAATATACAAGGTTGGTGATTAACATTACTGGAGAGATACAGTTTCTGAGGTGGATTGAATACACAAAGCAGTGGAATTTGAATTGGTCAGCGCCAAGAGATGAATGCAGTGTGCTTAATGCTTGCGGAAACTTTGGCAGCTGCAATGATAATAATATGCCGTTTCTGAGCAAATGTTTGCCAGGTTTCAAACCCCTGTATCTAGAGAAGTGGAATTCTGGAGACTTTTCAGATGGATGCGCAAGAAAGTCCGCATTATGCAACAAGAATGATACCTTCTTGAGCTTGAAGAAGATGAAAGTGGGAAACCCAGATTCAGAAATCAACGTTGACAATGAAACAGAATGCAGGAACGTGTGCTTAAATAGGTGCCGGTGCAAGGCTTATTCATATGCTAAATCTGAAAACAATAGTTATCGAAGGGAAGCGAGTACTTCATTGTGCAGAATATGGAACGTGGGAGATCTAAATAATCTTGAAGAAGAGTATAACAATATGGGCCAAGACCTCTGTGTTCGTGTTGCGTTGTCTGATTTAGAATCAACTGTAAGAGACTGCAAGCCTTGCGGCACAACCATCATCCCTTATCCCCTGAGCACAAGACTGGGTTGCGGTGACCCTTTGTACTTCCGTTTCAAGTGCAACTACTTCACAGGTCAGGTTAGCTTTGTGGGACCGAATGACGCCTTGTTTAGAGTTATTAGCATAAACTCAAGTACCCAGAGATTTTTCATCCAAGGCTTCCATACCGAAAATGTAGATAACAGTGATCCTAGAAACAGAGCGGCTAAGCAGATCAATCCATCGTTTCCATTTAAAGTAATCAGTCCAGGTAATGCTGACCCGGGTAATTTTAGTTCTGAAGTACTGTCATCCGGAGGTCCTAAAGTAGTGGAGATGGGTTGGGAGCTACCAATGGAACCAACCTGCACAACATCTGCAGACTGCAGGGATTGGGCACATTCAATTTGCAAGCGTGCACGAGATGGAAAGAAAAGATGCCTGTGCGAAACCAATTTTCGATGGAATGACTTGTTATTAAGATGTACTCAAGAAAATTGCCATTGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

653

Amino Acids

73.67

Weight (kDa)

6.97

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 98 - 192 1.5e-25 D-mannose binding lectin
S_locus_glycop PF00954 284 - 360 1.9e-18 S-locus glycoprotein domain
PAN_2 PF08276 382 - 451 6.9e-11 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 852
AasI GACNNNNNNGTC 1 cut(s) 662
Acc16I TGCGCA 1 cut(s) 1126
Acc36I ACCTGC 1 cut(s) 1820
AccB1I GGYRCC 1 cut(s) 1254
AccB7I CCANNNNNTGG 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 1670
AccIII TCCGGA 1 cut(s) 1760
AclI AACGTT 1 cut(s) 1212
AclWI GGATC 2 cut(s) 635, 1649
AcsI RAATTY 3 cut(s) 784, 961, 1099
AcuI CTGAAG 2 cut(s) 261, 1767
AcyI GRCGYC 1 cut(s) 1569
AfaI GTAC 7 cut(s) 404, 704, 1318, 1511, 1603, 1752, 1933
AfiI CCNNNNNNNGG 3 cut(s) 382, 617, 1264
AflIII ACRYGT 1 cut(s) 1239
AjnI CCWGG 3 cut(s) 553, 1066, 1714
AluBI AGCT 7 cut(s) 525, 576, 744, 1026, 1170, 1551, 1795
AluI AGCT 7 cut(s) 525, 576, 744, 1026, 1170, 1551, 1795
Alw21I GWGCWC 3 cut(s) 113, 1484, 1867
Alw26I GTCTC 2 cut(s) 1101, 1431
Alw44I GTGCAC 1 cut(s) 1863
AlwI GGATC 2 cut(s) 635, 1649
AlwNI CAGNNNCTG 1 cut(s) 931
Ama87I CYCGRG 1 cut(s) 1729
Aor13HI TCCGGA 1 cut(s) 1760
AoxI GGCC 1 cut(s) 1384
ApaLI GTGCAC 1 cut(s) 1863
ApeKI GCWGC 3 cut(s) 389, 1023, 1026
ApoI RAATTY 3 cut(s) 784, 961, 1099
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 2 cut(s) 979, 1127
AspS9I GGNCC 4 cut(s) 655, 1384, 1559, 1766
AsuC2I CCSGG 3 cut(s) 466, 1730, 1731
AsuHPI GGTGA 5 cut(s) 110, 228, 266, 913, 1511
AvaI CYCGRG 1 cut(s) 1729
AvaII GGWCC 3 cut(s) 655, 1559, 1766
BaeGI GKGCMC 2 cut(s) 1846, 1867
BaeI ACNNNNGTAYC 4 cut(s) 1067, 1100, 1585, 1618
BanI GGYRCC 1 cut(s) 1254
BauI CACGAG 1 cut(s) 1866
Bbv12I GWGCWC 3 cut(s) 113, 1484, 1867
BbvI GCAGC 3 cut(s) 376, 1013, 1035
BccI CCATC 8 cut(s) 83, 359, 1112, 1469, 1696, 1777, 1865, 1902
BceAI ACGGC 1 cut(s) 1030
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BciT130I CCWGG 3 cut(s) 555, 1068, 1716
BclI TGATCA 1 cut(s) 748
BcnI CCSGG 3 cut(s) 466, 1730, 1731
BcoDI GTCTC 2 cut(s) 1101, 1431
BfaI CTAG 3 cut(s) 135, 1088, 1659
BfmI CTRYAG 2 cut(s) 1824, 1831
BfoI RGCGCY 1 cut(s) 980
BfuAI ACCTGC 1 cut(s) 1820
BglII AGATCT 1 cut(s) 1348
BisI GCNGC 5 cut(s) 390, 1024, 1027, 1453, 1671
BlpI GCTNAGC 1 cut(s) 1674
BlsI GCNGC 5 cut(s) 391, 1025, 1028, 1454, 1672
BmcAI AGTACT 2 cut(s) 1318, 1752
Bme1390I CCNGG 6 cut(s) 466, 555, 1068, 1716, 1730, 1731
Bme18I GGWCC 3 cut(s) 655, 1559, 1766
BmeT110I CYCGRG 1 cut(s) 1729
BmgT120I GGNCC 4 cut(s) 655, 1384, 1559, 1766
BmiI GGNNCC 4 cut(s) 470, 1256, 1560, 1806
BmrFI CCNGG 6 cut(s) 466, 555, 1068, 1716, 1730, 1731
BmrI ACTGGG 1 cut(s) 1499
BmsI GCATC 4 cut(s) 376, 721, 1112, 1874
BmuI ACTGGG 1 cut(s) 1499
BpmI CTGGAG 2 cut(s) 936, 1125
Bpu10I CCTNAGC 1 cut(s) 1478
Bpu1102I GCTNAGC 1 cut(s) 1674
BpuEI CTTGAG 3 cut(s) 1186, 1582, 1920
BpuMI CCSGG 3 cut(s) 466, 1730, 1731
Bsa29I ATCGAT 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 1569
BsaJI CCNNGG 4 cut(s) 287, 554, 1621, 1729
BsaWI WCCGGW 1 cut(s) 1760
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 617, 1264
Bse118I RCCGGY 1 cut(s) 1257
Bse1I ACTGG 8 cut(s) 323, 343, 410, 644, 658, 710, 919, 1494
Bse3DI GCAATG 1 cut(s) 1036
BseAI TCCGGA 1 cut(s) 1760
BseBI CCWGG 3 cut(s) 555, 1068, 1716
BseCI ATCGAT 1 cut(s) 695
BseDI CCNNGG 4 cut(s) 287, 554, 1621, 1729
BseGI GGATG 7 cut(s) 391, 425, 564, 1127, 1464, 1617, 1757
BseLI CCNNNNNNNGG 3 cut(s) 382, 617, 1264
BseMI GCAATG 1 cut(s) 1036
BseMII CTCAG 4 cut(s) 25, 923, 1043, 1469
BseNI ACTGG 8 cut(s) 323, 343, 410, 644, 658, 710, 919, 1494
BseSI GKGCMC 2 cut(s) 1846, 1867
BseXI GCAGC 3 cut(s) 376, 1013, 1035
BsgI GTGCAG 2 cut(s) 1348, 1798
BshFI GGCC 1 cut(s) 1386
BshNI GGYRCC 1 cut(s) 1254
BshVI ATCGAT 1 cut(s) 695
BsiHKAI GWGCWC 3 cut(s) 113, 1484, 1867
BsiHKCI CYCGRG 1 cut(s) 1729
BsiSI CCGG 4 cut(s) 465, 1258, 1730, 1761
BslFI GGGAC 3 cut(s) 259, 1572, 1938
BslI CCNNNNNNNGG 3 cut(s) 382, 617, 1264
BsmAI GTCTC 2 cut(s) 1101, 1431
BsmFI GGGAC 3 cut(s) 259, 1572, 1938
BsmI GAATGC 2 cut(s) 995, 1235
BsnI GGCC 1 cut(s) 1386
BsoBI CYCGRG 1 cut(s) 1729
Bsp1286I GDGCHC 4 cut(s) 113, 1484, 1846, 1867
Bsp13I TCCGGA 1 cut(s) 1760
Bsp143I GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
Bsp1720I GCTNAGC 1 cut(s) 1674
BspANI GGCC 1 cut(s) 1386
BspCNI CTCAG 4 cut(s) 24, 924, 1044, 1470
BspDI ATCGAT 1 cut(s) 695
BspEI TCCGGA 1 cut(s) 1760
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 4 cut(s) 470, 1256, 1560, 1806
BspMAI CTGCAG 2 cut(s) 1828, 1835
BspMI ACCTGC 1 cut(s) 1820
BspPI GGATC 2 cut(s) 635, 1649
BspT107I GGYRCC 1 cut(s) 1254
BsrBI CCGCTC 1 cut(s) 1670
BsrDI GCAATG 1 cut(s) 1036
BsrFI RCCGGY 1 cut(s) 1257
BsrI ACTGG 8 cut(s) 323, 343, 410, 644, 658, 710, 919, 1494
BssAI RCCGGY 1 cut(s) 1257
BssECI CCNNGG 4 cut(s) 287, 554, 1621, 1729
BssMI GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
BssNI GRCGYC 1 cut(s) 1569
BssSI CACGAG 1 cut(s) 1866
BssT1I CCWWGG 1 cut(s) 1621
Bst2BI CACGAG 1 cut(s) 1866
Bst2UI CCWGG 3 cut(s) 555, 1068, 1716
Bst4CI ACNGT 8 cut(s) 145, 173, 295, 448, 927, 1432, 1652, 1755
Bst6I CTCTTC 1 cut(s) 1362
BstACI GRCGYC 1 cut(s) 1569
BstAPI GCANNNNNTGC 2 cut(s) 731, 1449
BstC8I GCNNGC 6 cut(s) 65, 620, 1009, 1445, 1859, 1863
BstDEI CTNAG 6 cut(s) 11, 479, 932, 1052, 1478, 1674
BstEII GGTNACC 2 cut(s) 368, 1499
BstF5I GGATG 7 cut(s) 391, 425, 564, 1127, 1464, 1617, 1757
BstH2I RGCGCY 1 cut(s) 980
BstHHI GCGC 2 cut(s) 979, 1127
BstKTI GATC 6 cut(s) 32, 643, 751, 1351, 1657, 1683
BstMAI GTCTC 2 cut(s) 1101, 1431
BstMBI GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
BstMWI GCNNNNNNNGC 2 cut(s) 731, 1449
BstNI CCWGG 3 cut(s) 555, 1068, 1716
BstNSI RCATGY 1 cut(s) 67
BstPI GGTNACC 2 cut(s) 368, 1499
BstSCI CCNGG 6 cut(s) 464, 553, 1066, 1714, 1728, 1729
BstSFI CTRYAG 2 cut(s) 1824, 1831
BstSLI GKGCMC 2 cut(s) 1846, 1867
BstV1I GCAGC 3 cut(s) 376, 1013, 1035
BstX2I RGATCY 2 cut(s) 640, 1348
BstYI RGATCY 2 cut(s) 640, 1348
Bsu15I ATCGAT 1 cut(s) 695
BsuRI GGCC 1 cut(s) 1386
BsuTUI ATCGAT 1 cut(s) 695
BtsCI GGATG 7 cut(s) 391, 425, 564, 1127, 1464, 1617, 1757
BtsI GCAGTG 4 cut(s) 490, 510, 962, 1000
BtsIMutI CAGTG 7 cut(s) 300, 336, 490, 510, 962, 1000, 1657
BveI ACCTGC 1 cut(s) 1820
Cac8I GCNNGC 6 cut(s) 65, 620, 1009, 1445, 1859, 1863
CaiI CAGNNNCTG 1 cut(s) 931
CciI TCATGA 1 cut(s) 490
CfoI GCGC 2 cut(s) 979, 1127
Cfr10I RCCGGY 1 cut(s) 1257
Cfr13I GGNCC 4 cut(s) 655, 1384, 1559, 1766
Cfr9I CCCGGG 1 cut(s) 1729
ClaI ATCGAT 1 cut(s) 695
CseI GACGC 3 cut(s) 448, 460, 1577
Csp6I GTAC 7 cut(s) 403, 703, 1317, 1510, 1602, 1751, 1932
CviAII CATG 6 cut(s) 48, 64, 377, 457, 491, 591
CviQI GTAC 7 cut(s) 403, 703, 1317, 1510, 1602, 1751, 1932
DdeI CTNAG 6 cut(s) 11, 479, 932, 1052, 1478, 1674
DpnI GATC 6 cut(s) 31, 642, 750, 1350, 1656, 1682
DpnII GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
DraI TTTAAA 1 cut(s) 1702
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
Eam1104I CTCTTC 1 cut(s) 1362
EarI CTCTTC 1 cut(s) 1362
EciI GGCGGA 2 cut(s) 729, 858
Eco130I CCWWGG 1 cut(s) 1621
Eco47I GGWCC 3 cut(s) 655, 1559, 1766
Eco57I CTGAAG 2 cut(s) 261, 1767
Eco88I CYCGRG 1 cut(s) 1729
Eco91I GGTNACC 2 cut(s) 368, 1499
EcoO109I RGGNCCY 1 cut(s) 1766
EcoO65I GGTNACC 2 cut(s) 368, 1499
EcoRI GAATTC 1 cut(s) 1099
EcoRII CCWGG 3 cut(s) 553, 1066, 1714
EcoT14I CCWWGG 1 cut(s) 1621
EcoT22I ATGCAT 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 1621
FaeI CATG 6 cut(s) 51, 67, 380, 460, 494, 594
FaqI GGGAC 3 cut(s) 259, 1572, 1938
FatI CATG 6 cut(s) 47, 63, 376, 456, 490, 590
FauI CCCGC 1 cut(s) 611
FauNDI CATATG 2 cut(s) 266, 1276
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 5 cut(s) 390, 1024, 1027, 1453, 1671
FokI GGATG 7 cut(s) 398, 412, 571, 1134, 1451, 1604, 1744
Fsp4HI GCNGC 5 cut(s) 390, 1024, 1027, 1453, 1671
FspBI CTAG 3 cut(s) 135, 1088, 1659
FspI TGCGCA 1 cut(s) 1126
GlaI GCGC 2 cut(s) 978, 1126
GluI GCNGC 5 cut(s) 390, 1024, 1027, 1453, 1671
GsuI CTGGAG 2 cut(s) 936, 1125
HaeII RGCGCY 1 cut(s) 980
HaeIII GGCC 1 cut(s) 1386
HapII CCGG 4 cut(s) 465, 1258, 1730, 1761
HgaI GACGC 3 cut(s) 448, 460, 1577
HhaI GCGC 2 cut(s) 979, 1127
Hin1I GRCGYC 1 cut(s) 1569
Hin1II CATG 6 cut(s) 51, 67, 380, 460, 494, 594
Hin6I GCGC 2 cut(s) 977, 1125
HinP1I GCGC 2 cut(s) 977, 1125
HincII GTYRAC 5 cut(s) 24, 328, 411, 585, 1216
HindII GTYRAC 5 cut(s) 24, 328, 411, 585, 1216
HindIII AAGCTT 1 cut(s) 574
HpaI GTTAAC 1 cut(s) 24
HpaII CCGG 4 cut(s) 465, 1258, 1730, 1761
HphI GGTGA 5 cut(s) 110, 228, 266, 913, 1511
Hpy166II GTNNAC 6 cut(s) 24, 328, 411, 585, 1216, 1865
Hpy188III TCNNGA 7 cut(s) 491, 1088, 1104, 1165, 1361, 1761, 1937
Hpy8I GTNNAC 6 cut(s) 24, 328, 411, 585, 1216, 1865
Hpy99I CGWCG 2 cut(s) 44, 173
HpyAV CCTTC 4 cut(s) 236, 243, 1171, 1299
HpyCH4III ACNGT 8 cut(s) 145, 173, 295, 448, 927, 1432, 1652, 1755
HpyCH4IV ACGT 4 cut(s) 39, 1212, 1239, 1341
HpyF10VI GCNNNNNNNGC 2 cut(s) 731, 1449
HpyF3I CTNAG 6 cut(s) 11, 479, 932, 1052, 1478, 1674
HpySE526I ACGT 4 cut(s) 39, 1212, 1239, 1341
Hsp92I GRCGYC 1 cut(s) 1569
Hsp92II CATG 6 cut(s) 51, 67, 380, 460, 494, 594
HspAI GCGC 2 cut(s) 977, 1125
Kpn2I TCCGGA 1 cut(s) 1760
Ksp22I TGATCA 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 24
Kzo9I GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
LmnI GCTCC 1 cut(s) 1792
Lsp1109I GCAGC 3 cut(s) 376, 1013, 1035
LweI GCATC 4 cut(s) 376, 721, 1112, 1874
MaeI CTAG 3 cut(s) 135, 1088, 1659
MaeII ACGT 4 cut(s) 39, 1212, 1239, 1341
MaeIII GTNAC 6 cut(s) 43, 145, 368, 448, 766, 1499
MalI GATC 6 cut(s) 31, 642, 750, 1350, 1656, 1682
MbiI CCGCTC 1 cut(s) 1670
MboI GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
MboII GAAGA 8 cut(s) 70, 219, 614, 622, 1186, 1189, 1376, 1379
MflI RGATCY 2 cut(s) 640, 1348
MhlI GDGCHC 4 cut(s) 113, 1484, 1846, 1867
MlyI GAGTC 4 cut(s) 241, 292, 440, 847
MmeI TCCRAC 3 cut(s) 363, 560, 628
Mph1103I ATGCAT 1 cut(s) 736
MroI TCCGGA 1 cut(s) 1760
MroXI GAANNNNTTC 1 cut(s) 696
MseI TTAA 7 cut(s) 23, 563, 906, 1002, 1247, 1701, 1925
MspA1I CMGCKG 1 cut(s) 1026
MspI CCGG 4 cut(s) 465, 1258, 1730, 1761
MspR9I CCNGG 6 cut(s) 466, 555, 1068, 1716, 1730, 1731
Mva1269I GAATGC 2 cut(s) 995, 1235
MvaI CCWGG 3 cut(s) 555, 1068, 1716
MwoI GCNNNNNNNGC 2 cut(s) 731, 1449
NciI CCSGG 3 cut(s) 466, 1730, 1731
NdeI CATATG 2 cut(s) 266, 1276
NdeII GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
NlaIII CATG 6 cut(s) 51, 67, 380, 460, 494, 594
NlaIV GGNNCC 4 cut(s) 470, 1256, 1560, 1806
NmuCI GTSAC 2 cut(s) 448, 1499
NsbI TGCGCA 1 cut(s) 1126
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 1 cut(s) 67
PaeI GCATGC 1 cut(s) 67
PagI TCATGA 1 cut(s) 490
PctI GAATGC 2 cut(s) 995, 1235
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 8 cut(s) 58, 179, 222, 461, 692, 697, 1199, 1424
PflMI CCANNNNNTGG 1 cut(s) 382
PfoI TCCNGGA 1 cut(s) 464
PkrI GCNGC 5 cut(s) 391, 1025, 1028, 1454, 1672
PleI GAGTC 4 cut(s) 241, 291, 439, 846
PpsI GAGTC 4 cut(s) 241, 291, 439, 846
PpuMI RGGWCCY 1 cut(s) 1766
PsiI TTATAA 1 cut(s) 852
Psp1406I AACGTT 1 cut(s) 1212
Psp5II RGGWCCY 1 cut(s) 1766
Psp6I CCWGG 3 cut(s) 553, 1066, 1714
PspEI GGTNACC 2 cut(s) 368, 1499
PspGI CCWGG 3 cut(s) 553, 1066, 1714
PspN4I GGNNCC 4 cut(s) 470, 1256, 1560, 1806
PspPI GGNCC 4 cut(s) 655, 1384, 1559, 1766
PspPPI RGGWCCY 1 cut(s) 1766
PsrI GAACNNNNNNTAC 2 cut(s) 1726, 1758
PstI CTGCAG 2 cut(s) 1828, 1835
PstNI CAGNNNCTG 1 cut(s) 931
PsuI RGATCY 2 cut(s) 640, 1348
PvuII CAGCTG 1 cut(s) 1026
RsaI GTAC 7 cut(s) 404, 704, 1318, 1511, 1603, 1752, 1933
RsaNI GTAC 7 cut(s) 403, 703, 1317, 1510, 1602, 1751, 1932
SaqAI TTAA 7 cut(s) 23, 563, 906, 1002, 1247, 1701, 1925
SatI GCNGC 5 cut(s) 390, 1024, 1027, 1453, 1671
Sau3AI GATC 6 cut(s) 29, 640, 748, 1348, 1654, 1680
Sau96I GGNCC 4 cut(s) 655, 1384, 1559, 1766
ScaI AGTACT 2 cut(s) 1318, 1752
SchI GAGTC 4 cut(s) 241, 292, 440, 847
ScrFI CCNGG 6 cut(s) 466, 555, 1068, 1716, 1730, 1731
SduI GDGCHC 4 cut(s) 113, 1484, 1846, 1867
SfaNI GCATC 4 cut(s) 376, 721, 1112, 1874
SfcI CTRYAG 2 cut(s) 1824, 1831
SinI GGWCC 3 cut(s) 655, 1559, 1766
SmaI CCCGGG 1 cut(s) 1731
SmlI CTYRAG 3 cut(s) 1165, 1597, 1935
SmoI CTYRAG 3 cut(s) 1165, 1597, 1935
SphI GCATGC 1 cut(s) 67
SspI AATATT 1 cut(s) 511
SspMI CTAG 3 cut(s) 135, 1088, 1659
StyD4I CCNGG 6 cut(s) 464, 553, 1066, 1714, 1728, 1729
StyI CCWWGG 1 cut(s) 1621
TaaI ACNGT 8 cut(s) 145, 173, 295, 448, 927, 1432, 1652, 1755
TaiI ACGT 4 cut(s) 42, 1215, 1242, 1344
TaqI TCGA 3 cut(s) 695, 1303, 1906
TaqII GACCGA 1 cut(s) 1576
TatI WGTACW 5 cut(s) 702, 1316, 1509, 1750, 1931
TauI GCSGC 2 cut(s) 1455, 1673
TfiI GAWTC 8 cut(s) 58, 179, 222, 461, 692, 697, 1199, 1424
Tru1I TTAA 7 cut(s) 23, 563, 906, 1002, 1247, 1701, 1925
Tru9I TTAA 7 cut(s) 23, 563, 906, 1002, 1247, 1701, 1925
TscAI CASTG 7 cut(s) 300, 343, 490, 510, 962, 1000, 1657
TseFI GTSAC 2 cut(s) 448, 1499
TseI GCWGC 3 cut(s) 389, 1023, 1026
Tsp45I GTSAC 2 cut(s) 448, 1499
TspDTI ATGAA 9 cut(s) 538, 579, 616, 1002, 1196, 1236, 1263, 1311, 1606
TspGWI ACGGA 2 cut(s) 428, 1505
TspMI CCCGGG 1 cut(s) 1729
TspRI CASTG 7 cut(s) 300, 343, 490, 510, 962, 1000, 1657
Van91I CCANNNNNTGG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 1863
VpaK11BI GGWCC 3 cut(s) 655, 1559, 1766
XapI RAATTY 3 cut(s) 784, 961, 1099
XbaI TCTAGA 1 cut(s) 1087
XceI RCATGY 1 cut(s) 67
XmaI CCCGGG 1 cut(s) 1729
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 3 cut(s) 135, 1088, 1659
ZrmI AGTACT 2 cut(s) 1318, 1752
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.