Rorug02G0486100

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000412
Physical Location & Seq
Reverse (-)
61568900 .. 61571705
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0486100.1

Sequence Viewer

Length: 2223 bp
ATGAAATCCCTAGTTCATCATATGATATCTATTATGGTTTTATGTTTATGGAGCTTGAGTACTAGACCAGCAGTAGCAGCAGAATCAGGAACAGCGCTGCCGATAGCAAAGCCGAATTGCCAAGAGAAATGCGGAAATGTCAGCATCCCGTACCCTTTCGGAATCGGGCCTGATAAAGATTGCTACCTTGAAGAGTGGTTTGAAATAGACTGCAACCAGTCTACTAGGCACAAGCCTTTCTTGAGGCTTACACAACAGGAGGTGCTGAGCATTTCTATCAAAGACGGCACGCTTCAGGTGAACAGCCCCGTTACTTTCTTCTGCAACGTAACAGGAAGTAGCCAGCCAGCAAATCTGACCGGAAGCCCTTTCGTGTACTCTCAGAGACAAAACAGATTCACCGCAGCGACTTGCGGCTTTGTCTCCTTGGTGAGCTCAGATCAATCGGTGGTTGGTGGGTGCAGGTCAATTTGTGATAAGCAGAATACCGGTAGATACTCTGATGGCTGTGATATTGGTACTAATTGTTGCCAGACGAGTATTCCTCCGTATCTCAGGGTTGTCACTGCTAGCGTAATGGTGGATGGACCAGCCAACATGACCGGCCGAAATGACAGTGGAAAGCCGGATTGCAGTGACTATGCATTCCTGGTTGATAAAGATTGGTTTGAGACTGCATCAAATTATAGCGCCGTTAGGAATATGCTCCATGTTCCTGTGGCACTAGAATGGAGTGTAATCAATGACACTAGTTCGTCGTTTGCATTTCAAGGAAATAAGAAATGCGATTTTGACACTTCTTTGGCATTTAATCGGTCAGTGCTTTCTTGCCGCTGCCCACAAGGGTTTGATGGAAATCCCTATCTTCTCCAACCTTGTCAAGACATTGATGAGTGCATAGATGGTACTAAACGATGCTGGCCCGGCAGCAAGTGCATGAACCTTCCTGGTTCGTTCAAATGCGAGGACGAAAAGGATACAATTAAATTGGCACTTGCAGGTCTTGGCTCGAGTCTTGGACTGTTATTATTACTTATTGCTGCATGGTGGGTACACAAACTGGTGAAGAAAAGGAAAAGCATTAAACGCAAGGAAATGTCATTCAAAAGAAACGGAGGTTTGTTGTTAGAAAGACAGTTATCATCTGGTGAAGTTAATCTTGAGAAAATTAAGTTGTTCAAATCAAAAGAGTTGGAGAAGTCCACTGATAATTTTCATGTTGATAGAATCCTTGGCAAAGGTGGCCAAGGGACTGTCTACAAAGGTATGCTAACAGATGGAAGAATCGTTGCAGTAAAAAGGTCTACAAAAGTGGACGAAGGTAGACTTTCACAGTTTATAAATGAGGTTGTTATTCTTTCACAAATCAACCACAGGAATGTGGTTAAACTATTGGGTTGTTGTTTGGAGACAGAGGTTCCTCTTTTGGTCTATGAGTTTATTCCGAATGGATCCCTTTCCCAATATATCCATGAGCAGCACGAGGATTTTCCACTTACGTGGGAATTGCGCTTAAGGATTGCCACGGAAATTGCAGGAGCTCTTTCCTACTTACACGGTGCAGCTTCAATTCCCATATTTCATAGAGACATCAAGTCGACAAATATATTGCTAGATGAAAAATACAGAGCAAAAATTGCCGACTTCGGAACTTCTAGATCAGTTGCCATAGACCAAACTCACTTGACCACACTTGTATATGGGACATTTGGTTACTTGGACCCAGAATACTTCCAGTCGAGCCAATTTACGGATAAAAGTGATGTGTATAGCTTTGGAGTGGTTCTTGTTGAACTCTTGAGTGGACAAAAACCAATCTCTGTGACAAGATCACAAGAAGAAGGTCGGAGTCTTGCAACACATTTCATTATTTTGATGCAGGATGATCGGCTATTTGAAGTTGTTGATGCTCGAGTTTTGAATGAAGCCTCTAAAGAAGATATCTTACTAGTTGCCAACCTTGCAAGAAGATGTTTGCATTTGAATGGTAGGAACCGCCCCACAATGAGAGAGGTAACAGCAGAGCTGGAGGGGATTCAAATGTCACAAAAAACAACTAATGGTGAGCAAAATTATGAAGAGGTTGATATTGTCAGGTATGATTCAATTGAGCCATGGGATGTTGCTTCAACTTCAACAGGAACCGTAACAGGAACAAGGTCAGGTGTTGCTGCTGGTCCGCCTTCATCACCATCGCAAGGAATTGCACTGTTATCTCTCTAG

Protein Analysis

740

Amino Acids

81.9

Weight (kDa)

6.31

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 40 - 101 1.2e-13 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 322 2.7e-06 Calcium-binding EGF domain
Pkinase PF00069 405 - 673 1.4e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 406 - 676 1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1340
Acc36I ACCTGC 2 cut(s) 453, 989
AccI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1598
AciI CCGC 6 cut(s) 132, 402, 414, 832, 1996, 2180
AclWI GGATC 2 cut(s) 1446, 1459
AcoI YGGCCR 2 cut(s) 604, 1243
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 6 cut(s) 61, 152, 377, 520, 907, 1053
AfeI AGCGCT 1 cut(s) 96
AfiI CCNNNNNNNGG 2 cut(s) 1750, 2198
AflII CTTAAG 1 cut(s) 1513
AgeI ACCGGT 1 cut(s) 488
AhdI GACNNNNNGTC 1 cut(s) 1594
AhlI ACTAGT 2 cut(s) 749, 1948
AjnI CCWGG 2 cut(s) 648, 946
AleI CACNNNNGTG 1 cut(s) 1498
AluBI AGCT 6 cut(s) 54, 435, 1541, 1565, 1773, 2026
AluI AGCT 6 cut(s) 54, 435, 1541, 1565, 1773, 2026
Alw21I GWGCWC 2 cut(s) 437, 1543
Alw26I GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
AlwI GGATC 2 cut(s) 1446, 1459
Ama87I CYCGRG 2 cut(s) 1009, 1911
Aor51HI AGCGCT 1 cut(s) 96
AoxI GGCC 4 cut(s) 167, 604, 920, 1243
ApeKI GCWGC 9 cut(s) 77, 97, 404, 834, 927, 1040, 1477, 1562, 2171
AsiGI ACCGGT 1 cut(s) 488
AspLEI GCGC 3 cut(s) 97, 692, 1512
AspS9I GGNCC 5 cut(s) 167, 587, 921, 1720, 2177
AsuC2I CCSGG 1 cut(s) 924
AsuHPI GGTGA 7 cut(s) 310, 391, 442, 1075, 1160, 2075, 2181
AsuNHI GCTAGC 1 cut(s) 569
AvaI CYCGRG 2 cut(s) 1009, 1911
AvaII GGWCC 3 cut(s) 587, 1720, 2177
BalI TGGCCA 1 cut(s) 1245
BamHI GGATCC 1 cut(s) 1451
BanII GRGCYC 2 cut(s) 437, 1543
BarI GAAGNNNNNNTAC 2 cut(s) 1929, 1961
BauI CACGAG 1 cut(s) 1481
Bbv12I GWGCWC 2 cut(s) 437, 1543
BbvI GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
BccI CCATC 6 cut(s) 497, 578, 845, 896, 1271, 2200
BceAI ACGGC 2 cut(s) 301, 677
BcgI CGANNNNNNTGC 2 cut(s) 1868, 1902
BciT130I CCWGG 2 cut(s) 650, 948
BciVI GTATCC 1 cut(s) 970
BcnI CCSGG 1 cut(s) 924
BcoDI GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
BcuI ACTAGT 2 cut(s) 749, 1948
BfoI RGCGCY 2 cut(s) 98, 693
BfrI CTTAAG 1 cut(s) 1513
BfuAI ACCTGC 2 cut(s) 453, 989
BfuI GTATCC 1 cut(s) 970
BlpI GCTNAGC 1 cut(s) 266
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 3 cut(s) 650, 924, 948
Bme18I GGWCC 3 cut(s) 587, 1720, 2177
BmeRI GACNNNNNGTC 1 cut(s) 1594
BmeT110I CYCGRG 2 cut(s) 1009, 1911
BmgT120I GGNCC 5 cut(s) 167, 587, 921, 1720, 2177
BmiI GGNNCC 5 cut(s) 1419, 1453, 1722, 1994, 2143
BmrFI CCNGG 3 cut(s) 650, 924, 948
BmsI GCATC 5 cut(s) 153, 686, 905, 1866, 1897
BmtI GCTAGC 1 cut(s) 573
BplI GAGNNNNNCTC 2 cut(s) 529, 561
BpmI CTGGAG 1 cut(s) 2048
Bpu1102I GCTNAGC 1 cut(s) 266
BpuEI CTTGAG 4 cut(s) 76, 262, 1181, 1819
BpuMI CCSGG 1 cut(s) 924
BsaAI YACGTR 1 cut(s) 1500
BsaBI GATNNNNATC 1 cut(s) 855
BsaJI CCNNGG 5 cut(s) 426, 1231, 1246, 1524, 2114
BsaWI WCCGGW 2 cut(s) 359, 488
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 2 cut(s) 1750, 2198
Bse118I RCCGGY 2 cut(s) 488, 602
Bse1I ACTGG 3 cut(s) 217, 1065, 1735
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 2 cut(s) 650, 948
BseDI CCNNGG 5 cut(s) 426, 1231, 1246, 1524, 2114
BseGI GGATG 4 cut(s) 144, 589, 1888, 2125
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 2 cut(s) 1750, 2198
BseMII CTCAG 4 cut(s) 257, 395, 450, 568
BseNI ACTGG 3 cut(s) 217, 1065, 1735
BseX3I CGGCCG 1 cut(s) 604
BseXI GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
BsgI GTGCAG 2 cut(s) 481, 1581
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 4 cut(s) 169, 606, 922, 1245
BshTI ACCGGT 1 cut(s) 488
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 437, 1543
BsiHKCI CYCGRG 2 cut(s) 1009, 1911
BsiSI CCGG 5 cut(s) 360, 489, 603, 626, 924
BslFI GGGAC 2 cut(s) 1264, 1717
BslI CCNNNNNNNGG 2 cut(s) 1750, 2198
BsmAI GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
BsmFI GGGAC 2 cut(s) 1264, 1717
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 169, 606, 922, 1245
BsoBI CYCGRG 2 cut(s) 1009, 1911
Bsp1286I GDGCHC 2 cut(s) 437, 1543
Bsp143I GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
Bsp1720I GCTNAGC 1 cut(s) 266
Bsp19I CCATGG 1 cut(s) 2114
BspACI CCGC 6 cut(s) 132, 402, 414, 832, 1996, 2180
BspANI GGCC 4 cut(s) 169, 606, 922, 1245
BspCNI CTCAG 4 cut(s) 258, 394, 449, 567
BspLI GGNNCC 5 cut(s) 1419, 1453, 1722, 1994, 2143
BspMI ACCTGC 2 cut(s) 453, 989
BspOI GCTAGC 1 cut(s) 573
BspPI GGATC 2 cut(s) 1446, 1459
BspTI CTTAAG 1 cut(s) 1513
BsrFI RCCGGY 2 cut(s) 488, 602
BsrI ACTGG 3 cut(s) 217, 1065, 1735
BssAI RCCGGY 2 cut(s) 488, 602
BssECI CCNNGG 5 cut(s) 426, 1231, 1246, 1524, 2114
BssMI GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
BssSI CACGAG 1 cut(s) 1481
BssT1I CCWWGG 4 cut(s) 426, 1231, 1246, 2114
Bst2BI CACGAG 1 cut(s) 1481
Bst2UI CCWGG 2 cut(s) 650, 948
Bst4CI ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1559, 2146, 2211
Bst6I CTCTTC 2 cut(s) 186, 2073
BstAFI CTTAAG 1 cut(s) 1513
BstAPI GCANNNNNTGC 2 cut(s) 933, 1637
BstBAI YACGTR 1 cut(s) 1500
BstC8I GCNNGC 5 cut(s) 290, 344, 348, 571, 920
BstDEI CTNAG 4 cut(s) 266, 381, 436, 554
BstDSI CCRYGG 2 cut(s) 1524, 2114
BstF5I GGATG 4 cut(s) 144, 589, 1888, 2125
BstH2I RGCGCY 2 cut(s) 98, 693
BstHHI GCGC 3 cut(s) 97, 692, 1512
BstKTI GATC 5 cut(s) 442, 1454, 1661, 1832, 1888
BstMAI GTCTC 5 cut(s) 379, 427, 665, 1403, 1581
BstMBI GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1637, 1961
BstNI CCWGG 2 cut(s) 650, 948
BstSCI CCNGG 3 cut(s) 648, 922, 946
BstV1I GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
BstX2I RGATCY 1 cut(s) 1451
BstXI CCANNNNNNTGG 1 cut(s) 1500
BstYI RGATCY 1 cut(s) 1451
BstZI CGGCCG 1 cut(s) 604
BsuI GTATCC 1 cut(s) 970
BsuRI GGCC 4 cut(s) 169, 606, 922, 1245
BtgI CCRYGG 2 cut(s) 1524, 2114
BtgZI GCGATG 1 cut(s) 2178
BtsCI GGATG 4 cut(s) 144, 589, 1888, 2125
BtsI GCAGTG 2 cut(s) 564, 640
BtsIMutI CAGTG 6 cut(s) 564, 622, 640, 825, 1203, 2207
BveI ACCTGC 2 cut(s) 453, 989
Cac8I GCNNGC 5 cut(s) 290, 344, 348, 571, 920
CfoI GCGC 3 cut(s) 97, 692, 1512
Cfr10I RCCGGY 2 cut(s) 488, 602
Cfr13I GGNCC 5 cut(s) 167, 587, 921, 1720, 2177
Csp6I GTAC 6 cut(s) 60, 151, 376, 519, 906, 1052
CspAI ACCGGT 1 cut(s) 488
CspCI CAANNNNNGTGG 2 cut(s) 1513, 1548
CviAII CATG 7 cut(s) 598, 710, 937, 1044, 1217, 1472, 2115
CviQI GTAC 6 cut(s) 60, 151, 376, 519, 906, 1052
DdeI CTNAG 4 cut(s) 266, 381, 436, 554
DpnI GATC 5 cut(s) 441, 1453, 1660, 1831, 1887
DpnII GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
DriI GACNNNNNGTC 1 cut(s) 1594
EaeI YGGCCR 2 cut(s) 604, 1243
EagI CGGCCG 1 cut(s) 604
Eam1104I CTCTTC 2 cut(s) 186, 2073
Eam1105I GACNNNNNGTC 1 cut(s) 1594
EarI CTCTTC 2 cut(s) 186, 2073
EciI GGCGGA 1 cut(s) 2169
Ecl136II GAGCTC 2 cut(s) 435, 1541
EclXI CGGCCG 1 cut(s) 604
Eco130I CCWWGG 4 cut(s) 426, 1231, 1246, 2114
Eco24I GRGCYC 2 cut(s) 437, 1543
Eco32I GATATC 2 cut(s) 27, 1942
Eco47I GGWCC 3 cut(s) 587, 1720, 2177
Eco47III AGCGCT 1 cut(s) 96
Eco52I CGGCCG 1 cut(s) 604
Eco53kI GAGCTC 2 cut(s) 435, 1541
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 2 cut(s) 1009, 1911
EcoICRI GAGCTC 2 cut(s) 435, 1541
EcoRII CCWGG 2 cut(s) 648, 946
EcoRV GATATC 2 cut(s) 27, 1942
EcoT14I CCWWGG 4 cut(s) 426, 1231, 1246, 2114
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 2 cut(s) 437, 1543
ErhI CCWWGG 4 cut(s) 426, 1231, 1246, 2114
FaeI CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1475, 2118
FalI AAGNNNNNCTT 6 cut(s) 224, 256, 1143, 1175, 1311, 1343
FaqI GGGAC 2 cut(s) 1264, 1717
FatI CATG 7 cut(s) 597, 709, 936, 1043, 1216, 1471, 2114
FauNDI CATATG 1 cut(s) 21
FblI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1598
FokI GGATG 4 cut(s) 131, 596, 1895, 2132
FriOI GRGCYC 2 cut(s) 437, 1543
GlaI GCGC 3 cut(s) 96, 691, 1511
GsuI CTGGAG 1 cut(s) 2048
HaeII RGCGCY 2 cut(s) 98, 693
HaeIII GGCC 4 cut(s) 169, 606, 922, 1245
HapII CCGG 5 cut(s) 360, 489, 603, 626, 924
HhaI GCGC 3 cut(s) 97, 692, 1512
Hin1II CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1475, 2118
Hin6I GCGC 3 cut(s) 95, 690, 1510
HinP1I GCGC 3 cut(s) 95, 690, 1510
HincII GTYRAC 1 cut(s) 1599
HindII GTYRAC 1 cut(s) 1599
HinfI GANTC 9 cut(s) 83, 162, 396, 1012, 1227, 1284, 1849, 2035, 2102
HpaII CCGG 5 cut(s) 360, 489, 603, 626, 924
HphI GGTGA 7 cut(s) 310, 391, 442, 1075, 1160, 2075, 2181
Hpy188I TCNGA 8 cut(s) 161, 357, 384, 439, 502, 1446, 1649, 1848
Hpy188III TCNNGA 6 cut(s) 87, 241, 881, 1160, 1656, 1798
Hpy99I CGWCG 1 cut(s) 760
HpyAV CCTTC 4 cut(s) 953, 1313, 1835, 2193
HpyCH4III ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1559, 2146, 2211
HpyCH4IV ACGT 2 cut(s) 327, 1499
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1637, 1961
HpyF3I CTNAG 4 cut(s) 266, 381, 436, 554
HpySE526I ACGT 2 cut(s) 327, 1499
Hsp92II CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1475, 2118
HspAI GCGC 3 cut(s) 95, 690, 1510
Kzo9I GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
LmnI GCTCC 3 cut(s) 51, 711, 1538
Lsp1109I GCAGC 9 cut(s) 84, 89, 416, 821, 939, 1027, 1489, 1574, 2158
LweI GCATC 5 cut(s) 153, 686, 905, 1866, 1897
MaeII ACGT 2 cut(s) 327, 1499
MaeIII GTNAC 9 cut(s) 310, 328, 562, 635, 1712, 1822, 2014, 2043, 2146
MalI GATC 5 cut(s) 441, 1453, 1660, 1831, 1887
MboI GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
MboII GAAGA 9 cut(s) 203, 310, 857, 1078, 1293, 1850, 1949, 1980, 2090
MfeI CAATTG 1 cut(s) 2106
MflI RGATCY 1 cut(s) 1451
MhlI GDGCHC 2 cut(s) 437, 1543
MlsI TGGCCA 1 cut(s) 1245
MluNI TGGCCA 1 cut(s) 1245
MlyI GAGTC 2 cut(s) 1021, 1858
MmeI TCCRAC 3 cut(s) 895, 1173, 1826
Mox20I TGGCCA 1 cut(s) 1245
Mph1103I ATGCAT 1 cut(s) 646
MscI TGGCCA 1 cut(s) 1245
MseI TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
MslI CAYNNNNRTG 4 cut(s) 727, 1377, 1498, 1983
Msp20I TGGCCA 1 cut(s) 1245
MspA1I CMGCKG 1 cut(s) 834
MspCI CTTAAG 1 cut(s) 1513
MspI CCGG 5 cut(s) 360, 489, 603, 626, 924
MspR9I CCNGG 3 cut(s) 650, 924, 948
MunI CAATTG 1 cut(s) 2106
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 2 cut(s) 650, 948
MwoI GCNNNNNNNGC 7 cut(s) 77, 924, 933, 1086, 1242, 1637, 1961
NciI CCSGG 1 cut(s) 924
NcoI CCATGG 1 cut(s) 2114
NdeI CATATG 1 cut(s) 21
NdeII GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
NheI GCTAGC 1 cut(s) 569
NlaIII CATG 7 cut(s) 601, 713, 940, 1047, 1220, 1475, 2118
NlaIV GGNNCC 5 cut(s) 1419, 1453, 1722, 1994, 2143
NmuCI GTSAC 4 cut(s) 562, 635, 1822, 2043
NsiI ATGCAT 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 1498
PaeR7I CTCGAG 2 cut(s) 1009, 1911
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 7 cut(s) 83, 162, 396, 1227, 1284, 2035, 2102
PinAI ACCGGT 1 cut(s) 488
PleI GAGTC 2 cut(s) 1020, 1857
PpsI GAGTC 2 cut(s) 1020, 1857
Ppu21I YACGTR 1 cut(s) 1500
PsiI TTATAA 1 cut(s) 1340
Psp124BI GAGCTC 2 cut(s) 437, 1543
Psp6I CCWGG 2 cut(s) 648, 946
PspGI CCWGG 2 cut(s) 648, 946
PspN4I GGNNCC 5 cut(s) 1419, 1453, 1722, 1994, 2143
PspPI GGNCC 5 cut(s) 167, 587, 921, 1720, 2177
PspXI VCTCGAGB 2 cut(s) 1009, 1911
PsuI RGATCY 1 cut(s) 1451
RsaI GTAC 6 cut(s) 61, 152, 377, 520, 907, 1053
RsaNI GTAC 6 cut(s) 60, 151, 376, 519, 906, 1052
RseI CAYNNNNRTG 4 cut(s) 727, 1377, 1498, 1983
SacI GAGCTC 2 cut(s) 437, 1543
SalI GTCGAC 1 cut(s) 1597
SaqAI TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
Sau3AI GATC 5 cut(s) 439, 1451, 1658, 1829, 1885
Sau96I GGNCC 5 cut(s) 167, 587, 921, 1720, 2177
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 2 cut(s) 1021, 1858
ScrFI CCNGG 3 cut(s) 650, 924, 948
SduI GDGCHC 2 cut(s) 437, 1543
SfaNI GCATC 5 cut(s) 153, 686, 905, 1866, 1897
Sfr274I CTCGAG 2 cut(s) 1009, 1911
SinI GGWCC 3 cut(s) 587, 1720, 2177
SlaI CTCGAG 2 cut(s) 1009, 1911
SmiMI CAYNNNNRTG 4 cut(s) 727, 1377, 1498, 1983
SmlI CTYRAG 7 cut(s) 55, 241, 1009, 1160, 1513, 1798, 1911
SmoI CTYRAG 7 cut(s) 55, 241, 1009, 1160, 1513, 1798, 1911
SpeI ACTAGT 2 cut(s) 749, 1948
SsiI CCGC 6 cut(s) 132, 402, 414, 832, 1996, 2180
SstI GAGCTC 2 cut(s) 437, 1543
StyD4I CCNGG 3 cut(s) 648, 922, 946
StyI CCWWGG 4 cut(s) 426, 1231, 1246, 2114
TaaI ACNGT 8 cut(s) 617, 1023, 1137, 1255, 1335, 1559, 2146, 2211
TaiI ACGT 2 cut(s) 330, 1502
TaqI TCGA 4 cut(s) 1010, 1598, 1739, 1912
TaqII GACCGA 1 cut(s) 804
TatI WGTACW 2 cut(s) 59, 375
TauI GCSGC 2 cut(s) 417, 834
TfiI GAWTC 7 cut(s) 83, 162, 396, 1227, 1284, 2035, 2102
Tru1I TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
Tru9I TTAA 7 cut(s) 810, 984, 1083, 1155, 1170, 1386, 1514
TscAI CASTG 6 cut(s) 571, 622, 640, 825, 1210, 2214
TseFI GTSAC 4 cut(s) 562, 635, 1822, 2043
TseI GCWGC 9 cut(s) 77, 97, 404, 834, 927, 1040, 1477, 1562, 2171
Tsp45I GTSAC 4 cut(s) 562, 635, 1822, 2043
TspGWI ACGGA 4 cut(s) 537, 1128, 1541, 1766
TspRI CASTG 6 cut(s) 571, 622, 640, 825, 1210, 2214
Vha464I CTTAAG 1 cut(s) 1513
VpaK11BI GGWCC 3 cut(s) 587, 1720, 2177
XbaI TCTAGA 1 cut(s) 1655
XhoI CTCGAG 2 cut(s) 1009, 1911
XmiI GTMKAC 5 cut(s) 221, 1257, 1304, 1324, 1598
ZrmI AGTACT 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.