FvH4_6g44244

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000412
Physical Location & Seq
Reverse (-)
34103739 .. 34107959
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44244.t1

Sequence Viewer

Length: 3057 bp
ATGATAGCAAGCACACATTTATTCATTACTTGGTTTGCATATAGCATGGTATCTTACATCTTGTTCTTGTATGTTGTACTATTGCTATGCTCTTATGACAGCCTATTTTGTGCTGCTAGAGATGAATTAACATATGATGATCCAGTTATCGATAATGGATCAGATGCTCTTGTTTCAGTAGGAGGAGAATTTGAACTAGGTTTCTTTCCTACTACTACAGGGAAAAGATATGTGGGCATATGGTATCATAAGATCAAACCAAGAACAGTTGTATGGGTTGCCAACCGAGAGGGACTACCAGCCAATTCCACTGGAGTTCTTACAATTGATGAAGAAGGTAACCTCTGCGTGTTGGATAGAGATACTGGAAAGCTTTATTGGTCAGCAGAAGTTGGAACATCTTCGTCTTTTAACATGACAGTGATGACAGTACAGATAACGGATTATGGGAACCTGGTTTTGAGGGAAACTGGTGATGAGTTGACAGCAGATCCTTTGTGGCAGAGCTTTCAATACCCAACTGATACATTCATTCCTGGGATGCTAATGGATGCTAACTTGGAGTTGACTTCATGGAGCGACAAAGATGACCCAAGAACTGGGAACTACACCTTCAAGCTAGATCAGGGAGGGAACCTGTTTACCATCTTAAACAAATCGGTTCCTTACTGGAAAAGTGGAGAGCCAGGTATATACTTGAGCTCAGATGAGATGCGTCCTGAGGTAGCTTACTTGTTATTAAACTTAAGCAAGAATTCTCTTTATAGAACTCAGTCACAATTTAACTACAGTTATACAAGATTGGTGATCAATTCTAATGGAGTGCTCAATGGTTTGACCTGGATCGACACCATTAAGCAGTGGAGTGTCTTCTTGTCTGAGCCAAAAGATCAATGCAGTGTGATAAATGCATGTGGAAACTTTGGCAGCTGTAATATTGATAATACCCCGCTAGTGTGCAAATGCTTGCCAGGTTTCAACCCCCAGTTTATAGAGAAGTGGAAATCCGGAGATTTTTCGGGTGGTTGCACAAGACAGTCGTCATGTAGCGAGAATGACACCTTCTTAAGCATAAAGGTGAAAAAGGCAGGAAGATATGGTTCATCATCCTATGTTACCAGTGATACAGAATGCAAGAATACGTGCTTTGGTAGTTGCCAGTGCCAGGCTTACACATCTACTTCAGCTGTAAACTTCACTGCTCGAAGGGATATTCTCAAGAATGTCATTTGTTATATATGGACCACGGATCTAAATAATCTGGTAGAGGACTCTGGTAACAATGGCAAGAATCTCAATGTTCGAGTTTCCCCGTCTGATTTAGGATCTATTGTAAGAGACTGTCAGCCTTGTGGCACAACCACCATTCCTTATCCCCTGAGCACTAGATCAGATTGTGGTGACCCCATGTATTTAAGTTTCTGGTGCAACACCTCCAGTAGTGAATTCATCTTCCATGGATCAGATGACACCTCCTTTAGAGTTATAAATATCAGCCGAAGTACACAACAATTCATTATCAAATCCAAAAATAAGGATTATTGTGATTCTAGAAACAGAAGACCAAACATTTCCCTTCCGTTTAAGGTAAACACTTGGTGCAGTTCTGACCTGACGAGGAATTTTAATTCTGCCATAGACTCATCCAGCAGTCCTTCTGTAATAGAGATGGGTTGGGAGCCACCCCTGGAACCAACCTGCACTACATTAAAAGACTGCAGGGACTGGCCAAATTCTACTTGCAATCCAGTAAGCGGAGGAAAGAAAAAATGCTTTTGCAATACAAATTTTCAATGGAACGGCATCACTTTAAGCTGTACTCAAGTAGGAAGTCTACAAGCGCTATCAAATAAGAGTTCAAGGAAAAAATTGCTATTGCCTCTGCTTGTTGTGGCAGCTATAAGTGGGATAATTCTAGCATGCATCATTGGTTTTTGTATATGGAGAAGAAAGATGACTAAGAGACAAGATCAAGTAAGTCGTCCGCAGCTTGATAGTGAAAGAAGAGTCAAAGAGCTCATAGACACAAGTGACTTCAATGAAGAAGCTGATAAAGGCATAGATGTACCTTTTTTTGATTTGCAAACAATACTAGTTGCTACAGATAATTTCTCAATTGCAAACAAGCTTGGACAAGGGGGGTATGGGCCTGTTTACAAGGGGATCTTTCCTGGAGGTCAAGAAATTGCTGTAAAGAGGCTATCAAAAGTTTCAGGACAAGGCTTACAGGAATTTAAGAATGAGGTGGTGCTAATCGCCAAACTACAACATCGTAATCTTGTTAGACTCAGAGGATATTGCATGAAAGGAGAAGAAAAGATTTTACTGTACGAGTACATGCCTAACAAAAGCTTAGATTCCTTTATATTTGATCATACCCAAAGCCTGTTTCTAAATTGGGAGATGCGGTTTAACATTATTTTGGGAATTGCTCGAGGACTTGTTTATCTTCATCAAGATTCCAGGTTGAGGATCATTCACAGAGATTTGAAAACCAGCAATGTTCTTTTGGATGAAGAAATGAACCCCAAAATATCTGACTTCGGCTTGGCCAGGATTGTTGGAGGCAAAGAAACCGAGGCAAACACAAATACAGTTGTAGGAACCTATGGTTATATGTCACCAGAATATGCATTAGATGGAAATTTCTCTGTCAAATCAGATGTCTTTAGTTTTGGTGTGGTTCTTCTTGAGATAATCAGTGGAAAAAAGAATGCAGGATTTTATCAGTCCAAACAGACCTTCAGTCTACTTAGTTATACATGGGGACTCTGGACAGAAGACAAGGTGTTGGATTTAATGGACAAGAATTTGGAAGAAAGTTGTAACAGAAGTGAATTTATGAAGTGTGTAAATGTTGGGCTATTATGCGTACAAGAAGATCCCGTTGATCGCCCTACAATGTCAAATGTCATCACCTTGCTTGACAGTGAAGCTGCAATCCCTGCAACTCCTAAACAACCAGCCTTTTTCATAAGGAGAGGCAACTCTAGCACAGCTTCCTCTTCTACTAAGCCAGAACCAAATTCTGAAATAACTATTCTGGAAGGTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1019

Amino Acids

113.95

Weight (kDa)

5.74

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 89 - 187 1.3e-22 D-mannose binding lectin
S_locus_glycop PF00954 248 - 329 1.3e-19 S-locus glycoprotein domain
PAN_2 PF08276 349 - 420 4e-07 PAN-like domain
Pkinase PF00069 705 - 971 8.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 706 - 973 6.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1487
Acc36I ACCTGC 1 cut(s) 1706
AccB7I CCANNNNNTGG 1 cut(s) 599
AccI GTMKAC 2 cut(s) 1834, 2749
AccIII TCCGGA 1 cut(s) 1007
AciI CCGC 4 cut(s) 950, 1755, 1985, 2407
AcoI YGGCCR 2 cut(s) 1727, 2550
AcuI CTGAAG 2 cut(s) 1167, 2728
AdeI CACNNNGTG 1 cut(s) 1599
AfaI GTAC 8 cut(s) 78, 432, 1504, 1819, 2067, 2330, 2336, 2874
AfeI AGCGCT 1 cut(s) 1842
AfiI CCNNNNNNNGG 4 cut(s) 599, 1165, 1754, 2469
AflII CTTAAG 2 cut(s) 745, 1066
AgsI TTSAA 8 cut(s) 194, 512, 616, 979, 1793, 1860, 2038, 2491
AhdI GACNNNNNGTC 1 cut(s) 2745
AhlI ACTAGT 1 cut(s) 2092
AjuI GAANNNNNNNTTGG 6 cut(s) 361, 393, 2373, 2405, 2726, 2758
Alw21I GWGCWC 4 cut(s) 704, 828, 1385, 2019
Alw26I GTCTC 2 cut(s) 1332, 1957
AlwNI CAGNNNCTG 1 cut(s) 1725
Ama87I CYCGRG 1 cut(s) 2433
Aor13HI TCCGGA 1 cut(s) 1007
Aor51HI AGCGCT 1 cut(s) 1842
AoxI GGCC 3 cut(s) 1727, 2147, 2550
ApeKI GCWGC 5 cut(s) 113, 927, 1895, 1987, 2936
ArsI GACNNNNNNTTYG 2 cut(s) 2649, 2681
Asp700I GAANNNNTTC 1 cut(s) 400
AspLEI GCGC 1 cut(s) 1843
AspS9I GGNCC 2 cut(s) 1242, 2147
AsuHPI GGTGA 6 cut(s) 485, 817, 1090, 1412, 2613, 2908
AvaI CYCGRG 1 cut(s) 2433
AvaII GGWCC 1 cut(s) 1242
AxyI CCTNAGG 1 cut(s) 720
BalI TGGCCA 2 cut(s) 1729, 2552
BanII GRGCYC 2 cut(s) 704, 2019
BbsI GAAGAC 3 cut(s) 862, 1567, 2787
Bbv12I GWGCWC 4 cut(s) 704, 828, 1385, 2019
BbvI GCAGC 5 cut(s) 100, 939, 1907, 1999, 2923
BccI CCATC 3 cut(s) 653, 1663, 2633
BceAI ACGGC 1 cut(s) 1816
BclI TGATCA 2 cut(s) 807, 2371
BcoDI GTCTC 2 cut(s) 1332, 1957
BcuI ACTAGT 1 cut(s) 2092
BfaI CTAG 9 cut(s) 117, 197, 620, 953, 1386, 1551, 1916, 2093, 2991
BfmI CTRYAG 4 cut(s) 216, 787, 1717, 2100
BfoI RGCGCY 1 cut(s) 1844
BfrI CTTAAG 2 cut(s) 745, 1066
BfuAI ACCTGC 1 cut(s) 1706
BisI GCNGC 5 cut(s) 114, 928, 1896, 1988, 2937
BlsI GCNGC 5 cut(s) 115, 929, 1897, 1989, 2938
Bme18I GGWCC 1 cut(s) 1242
BmeRI GACNNNNNGTC 1 cut(s) 2745
BmeT110I CYCGRG 1 cut(s) 2433
BmgT120I GGNCC 2 cut(s) 1242, 2147
BmiI GGNNCC 6 cut(s) 452, 635, 663, 1680, 1692, 2605
BmrI ACTGGG 2 cut(s) 609, 979
BmsI GCATC 7 cut(s) 154, 531, 541, 702, 1812, 1932, 2394
BmuI ACTGGG 2 cut(s) 609, 979
BoxI GACNNNNGTC 1 cut(s) 1039
BpiI GAAGAC 3 cut(s) 862, 1567, 2787
BpmI CTGGAG 3 cut(s) 333, 1420, 2193
Bpu10I CCTNAGC 1 cut(s) 1379
BpuEI CTTGAG 4 cut(s) 718, 1202, 1806, 2711
Bsa29I ATCGAT 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 1143
BsaJI CCNNGG 5 cut(s) 536, 1245, 1456, 1686, 2577
BsaWI WCCGGW 1 cut(s) 1007
BsaXI ACNNNNNCTCC 2 cut(s) 2393, 2423
Bsc4I CCNNNNNNNGG 4 cut(s) 599, 1165, 1754, 2469
Bse21I CCTNAGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 2506
BseAI TCCGGA 1 cut(s) 1007
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 5 cut(s) 536, 1245, 1456, 1686, 2577
BseGI GGATG 5 cut(s) 546, 556, 1106, 1643, 2518
BseLI CCNNNNNNNGG 4 cut(s) 599, 1165, 1754, 2469
BseMI GCAATG 1 cut(s) 2506
BseMII CTCAG 6 cut(s) 711, 717, 785, 870, 1370, 2302
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 5 cut(s) 100, 939, 1907, 1999, 2923
BsgI GTGCAG 2 cut(s) 1621, 1684
BshFI GGCC 3 cut(s) 1729, 2149, 2552
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 4 cut(s) 704, 828, 1385, 2019
BsiHKCI CYCGRG 1 cut(s) 2433
BsiSI CCGG 1 cut(s) 1008
BslFI GGGAC 3 cut(s) 306, 1736, 2781
BslI CCNNNNNNNGG 4 cut(s) 599, 1165, 1754, 2469
BsmAI GTCTC 2 cut(s) 1332, 1957
BsmFI GGGAC 3 cut(s) 306, 1736, 2781
BsmI GAATGC 2 cut(s) 1136, 2719
BsnI GGCC 3 cut(s) 1729, 2149, 2552
BsoBI CYCGRG 1 cut(s) 2433
Bsp1286I GDGCHC 4 cut(s) 704, 828, 1385, 2019
Bsp13I TCCGGA 1 cut(s) 1007
Bsp19I CCATGG 1 cut(s) 1456
BspACI CCGC 4 cut(s) 950, 1755, 1985, 2407
BspANI GGCC 3 cut(s) 1729, 2149, 2552
BspCNI CTCAG 6 cut(s) 712, 716, 784, 871, 1371, 2301
BspDI ATCGAT 1 cut(s) 150
BspEI TCCGGA 1 cut(s) 1007
BspLI GGNNCC 6 cut(s) 452, 635, 663, 1680, 1692, 2605
BspMAI CTGCAG 1 cut(s) 1721
BspMI ACCTGC 1 cut(s) 1706
BspTI CTTAAG 2 cut(s) 745, 1066
BsrDI GCAATG 1 cut(s) 2506
BssECI CCNNGG 5 cut(s) 536, 1245, 1456, 1686, 2577
BssT1I CCWWGG 1 cut(s) 1456
Bst4CI ACNGT 9 cut(s) 268, 421, 430, 791, 1038, 1343, 2328, 2596, 2930
Bst6I CTCTTC 2 cut(s) 1999, 3010
BstAFI CTTAAG 2 cut(s) 745, 1066
BstAPI GCANNNNNTGC 1 cut(s) 2945
BstBAI YACGTR 1 cut(s) 1143
BstC8I GCNNGC 3 cut(s) 10, 968, 1921
BstDSI CCRYGG 2 cut(s) 1245, 1456
BstEII GGTNACC 2 cut(s) 338, 1400
BstF5I GGATG 5 cut(s) 546, 556, 1106, 1643, 2518
BstH2I RGCGCY 1 cut(s) 1844
BstHHI GCGC 1 cut(s) 1843
BstMAI GTCTC 2 cut(s) 1332, 1957
BstMWI GCNNNNNNNGC 2 cut(s) 2945, 2991
BstNSI RCATGY 3 cut(s) 915, 1923, 2341
BstPAI GACNNNNGTC 1 cut(s) 1039
BstPI GGTNACC 2 cut(s) 338, 1400
BstSFI CTRYAG 4 cut(s) 216, 787, 1717, 2100
BstV1I GCAGC 5 cut(s) 100, 939, 1907, 1999, 2923
BstV2I GAAGAC 3 cut(s) 862, 1567, 2787
BstX2I RGATCY 5 cut(s) 490, 1249, 1325, 2163, 2881
BstYI RGATCY 5 cut(s) 490, 1249, 1325, 2163, 2881
Bsu15I ATCGAT 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 3 cut(s) 1729, 2149, 2552
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 2 cut(s) 1245, 1456
BtsCI GGATG 5 cut(s) 546, 556, 1106, 1643, 2518
BtsI GCAGTG 3 cut(s) 866, 904, 1197
BtsIMutI CAGTG 9 cut(s) 309, 426, 866, 904, 1126, 1166, 1197, 2707, 2935
BveI ACCTGC 1 cut(s) 1706
Cac8I GCNNGC 3 cut(s) 10, 968, 1921
CaiI CAGNNNCTG 1 cut(s) 1725
CfoI GCGC 1 cut(s) 1843
Cfr13I GGNCC 2 cut(s) 1242, 2147
ClaI ATCGAT 1 cut(s) 150
CseI GACGC 1 cut(s) 704
CsiI ACCWGGT 1 cut(s) 453
Csp6I GTAC 8 cut(s) 77, 431, 1503, 1818, 2066, 2329, 2335, 2873
CviQI GTAC 8 cut(s) 77, 431, 1503, 1818, 2066, 2329, 2335, 2873
DraIII CACNNNGTG 1 cut(s) 1599
DriI GACNNNNNGTC 1 cut(s) 2745
EaeI YGGCCR 2 cut(s) 1727, 2550
Eam1104I CTCTTC 2 cut(s) 1999, 3010
Eam1105I GACNNNNNGTC 1 cut(s) 2745
EarI CTCTTC 2 cut(s) 1999, 3010
Ecl136II GAGCTC 2 cut(s) 702, 2017
Eco130I CCWWGG 1 cut(s) 1456
Eco24I GRGCYC 2 cut(s) 704, 2019
Eco47I GGWCC 1 cut(s) 1242
Eco47III AGCGCT 1 cut(s) 1842
Eco53kI GAGCTC 2 cut(s) 702, 2017
Eco57I CTGAAG 2 cut(s) 1167, 2728
Eco81I CCTNAGG 1 cut(s) 720
Eco88I CYCGRG 1 cut(s) 2433
Eco91I GGTNACC 2 cut(s) 338, 1400
EcoICRI GAGCTC 2 cut(s) 702, 2017
EcoO65I GGTNACC 2 cut(s) 338, 1400
EcoRI GAATTC 2 cut(s) 754, 1445
EcoT14I CCWWGG 1 cut(s) 1456
EcoT22I ATGCAT 3 cut(s) 913, 1925, 2635
EcoT38I GRGCYC 2 cut(s) 704, 2019
ErhI CCWWGG 1 cut(s) 1456
FalI AAGNNNNNCTT 2 cut(s) 2150, 2182
FaqI GGGAC 3 cut(s) 306, 1736, 2781
FauI CCCGC 1 cut(s) 957
FauNDI CATATG 2 cut(s) 133, 239
FbaI TGATCA 2 cut(s) 807, 2371
FblI GTMKAC 2 cut(s) 1834, 2749
Fnu4HI GCNGC 5 cut(s) 114, 928, 1896, 1988, 2937
FokI GGATG 5 cut(s) 553, 563, 1093, 1630, 2525
FriOI GRGCYC 2 cut(s) 704, 2019
Fsp4HI GCNGC 5 cut(s) 114, 928, 1896, 1988, 2937
FspBI CTAG 9 cut(s) 117, 197, 620, 953, 1386, 1551, 1916, 2093, 2991
GlaI GCGC 1 cut(s) 1842
GluI GCNGC 5 cut(s) 114, 928, 1896, 1988, 2937
GsuI CTGGAG 3 cut(s) 333, 1420, 2193
HaeII RGCGCY 1 cut(s) 1844
HaeIII GGCC 3 cut(s) 1729, 2149, 2552
HapII CCGG 1 cut(s) 1008
HgaI GACGC 1 cut(s) 704
HhaI GCGC 1 cut(s) 1843
Hin6I GCGC 1 cut(s) 1841
HinP1I GCGC 1 cut(s) 1841
HincII GTYRAC 2 cut(s) 483, 567
HindII GTYRAC 2 cut(s) 483, 567
HindIII AAGCTT 3 cut(s) 371, 2126, 2350
HinfI GANTC 9 cut(s) 1271, 1291, 1547, 1640, 2007, 2286, 2357, 2459, 2769
HpaII CCGG 1 cut(s) 1008
HphI GGTGA 6 cut(s) 485, 817, 1090, 1412, 2613, 2908
Hpy166II GTNNAC 9 cut(s) 483, 567, 642, 1192, 1505, 1591, 1835, 2155, 2750
Hpy8I GTNNAC 9 cut(s) 483, 567, 642, 1192, 1505, 1591, 1835, 2155, 2750
HpyAV CCTTC 8 cut(s) 329, 622, 1072, 1200, 1586, 1665, 2752, 3041
HpyCH4III ACNGT 9 cut(s) 268, 421, 430, 791, 1038, 1343, 2328, 2596, 2930
HpyCH4IV ACGT 1 cut(s) 1142
HpyF10VI GCNNNNNNNGC 2 cut(s) 2945, 2991
HpySE526I ACGT 1 cut(s) 1142
HspAI GCGC 1 cut(s) 1841
Kpn2I TCCGGA 1 cut(s) 1007
Ksp22I TGATCA 2 cut(s) 807, 2371
LmnI GCTCC 2 cut(s) 576, 1678
Lsp1109I GCAGC 5 cut(s) 100, 939, 1907, 1999, 2923
LweI GCATC 7 cut(s) 154, 531, 541, 702, 1812, 1932, 2394
MabI ACCWGGT 1 cut(s) 453
MaeI CTAG 9 cut(s) 117, 197, 620, 953, 1386, 1551, 1916, 2093, 2991
MaeII ACGT 1 cut(s) 1142
MaeIII GTNAC 8 cut(s) 338, 774, 1114, 1277, 1400, 2030, 2619, 2825
MfeI CAATTG 2 cut(s) 324, 2115
MflI RGATCY 5 cut(s) 490, 1249, 1325, 2163, 2881
MhlI GDGCHC 4 cut(s) 704, 828, 1385, 2019
MlsI TGGCCA 2 cut(s) 1729, 2552
MluNI TGGCCA 2 cut(s) 1729, 2552
MlyI GAGTC 5 cut(s) 1265, 1634, 2016, 2280, 2763
MmeI TCCRAC 4 cut(s) 333, 373, 2542, 2772
Mox20I TGGCCA 2 cut(s) 1729, 2552
Mph1103I ATGCAT 3 cut(s) 913, 1925, 2635
MroI TCCGGA 1 cut(s) 1007
MroXI GAANNNNTTC 1 cut(s) 400
MscI TGGCCA 2 cut(s) 1729, 2552
MslI CAYNNNNRTG 2 cut(s) 419, 1076
Msp20I TGGCCA 2 cut(s) 1729, 2552
MspA1I CMGCKG 2 cut(s) 930, 1187
MspCI CTTAAG 2 cut(s) 745, 1066
MspI CCGG 1 cut(s) 1008
MunI CAATTG 2 cut(s) 324, 2115
Mva1269I GAATGC 2 cut(s) 1136, 2719
MwoI GCNNNNNNNGC 2 cut(s) 2945, 2991
NcoI CCATGG 1 cut(s) 1456
NdeI CATATG 2 cut(s) 133, 239
NlaIV GGNNCC 6 cut(s) 452, 635, 663, 1680, 1692, 2605
NmuCI GTSAC 4 cut(s) 774, 1400, 2030, 2619
NsiI ATGCAT 3 cut(s) 913, 1925, 2635
NspI RCATGY 3 cut(s) 915, 1923, 2341
PaeI GCATGC 1 cut(s) 1923
PaeR7I CTCGAG 1 cut(s) 2433
PctI GAATGC 2 cut(s) 1136, 2719
PdmI GAANNNNTTC 1 cut(s) 400
PfeI GAWTC 4 cut(s) 1291, 1547, 2357, 2459
PflMI CCANNNNNTGG 1 cut(s) 599
PfoI TCCNGGA 1 cut(s) 2170
PkrI GCNGC 5 cut(s) 115, 929, 1897, 1989, 2938
PleI GAGTC 5 cut(s) 1265, 1634, 2015, 2280, 2763
PpsI GAGTC 5 cut(s) 1265, 1634, 2015, 2280, 2763
Ppu21I YACGTR 1 cut(s) 1143
PshAI GACNNNNGTC 1 cut(s) 1039
PsiI TTATAA 1 cut(s) 1487
Psp124BI GAGCTC 2 cut(s) 704, 2019
PspEI GGTNACC 2 cut(s) 338, 1400
PspN4I GGNNCC 6 cut(s) 452, 635, 663, 1680, 1692, 2605
PspPI GGNCC 2 cut(s) 1242, 2147
PspXI VCTCGAGB 1 cut(s) 2433
PstI CTGCAG 1 cut(s) 1721
PstNI CAGNNNCTG 1 cut(s) 1725
PsuI RGATCY 5 cut(s) 490, 1249, 1325, 2163, 2881
PvuII CAGCTG 2 cut(s) 930, 1187
RsaI GTAC 8 cut(s) 78, 432, 1504, 1819, 2067, 2330, 2336, 2874
RsaNI GTAC 8 cut(s) 77, 431, 1503, 1818, 2066, 2329, 2335, 2873
RseI CAYNNNNRTG 2 cut(s) 419, 1076
SacI GAGCTC 2 cut(s) 704, 2019
SatI GCNGC 5 cut(s) 114, 928, 1896, 1988, 2937
Sau96I GGNCC 2 cut(s) 1242, 2147
SchI GAGTC 5 cut(s) 1265, 1634, 2016, 2280, 2763
SduI GDGCHC 4 cut(s) 704, 828, 1385, 2019
SexAI ACCWGGT 1 cut(s) 453
SfaNI GCATC 7 cut(s) 154, 531, 541, 702, 1812, 1932, 2394
SfcI CTRYAG 4 cut(s) 216, 787, 1717, 2100
Sfr274I CTCGAG 1 cut(s) 2433
SinI GGWCC 1 cut(s) 1242
SlaI CTCGAG 1 cut(s) 2433
SmiMI CAYNNNNRTG 2 cut(s) 419, 1076
SmlI CTYRAG 7 cut(s) 697, 745, 1066, 1217, 1821, 2433, 2690
SmoI CTYRAG 7 cut(s) 697, 745, 1066, 1217, 1821, 2433, 2690
SpeI ACTAGT 1 cut(s) 2092
SphI GCATGC 1 cut(s) 1923
SsiI CCGC 4 cut(s) 950, 1755, 1985, 2407
SspI AATATT 1 cut(s) 937
SspMI CTAG 9 cut(s) 117, 197, 620, 953, 1386, 1551, 1916, 2093, 2991
SstI GAGCTC 2 cut(s) 704, 2019
StyI CCWWGG 1 cut(s) 1456
TaaI ACNGT 9 cut(s) 268, 421, 430, 791, 1038, 1343, 2328, 2596, 2930
TaiI ACGT 1 cut(s) 1145
TaqI TCGA 5 cut(s) 150, 846, 1204, 1303, 2434
TatI WGTACW 5 cut(s) 76, 430, 1502, 1817, 2334
TfiI GAWTC 4 cut(s) 1291, 1547, 2357, 2459
TscAI CASTG 9 cut(s) 316, 426, 866, 904, 1126, 1166, 1204, 2707, 2935
TseFI GTSAC 4 cut(s) 774, 1400, 2030, 2619
TseI GCWGC 5 cut(s) 113, 927, 1895, 1987, 2936
Tsp45I GTSAC 4 cut(s) 774, 1400, 2030, 2619
TspGWI ACGGA 3 cut(s) 455, 1262, 1569
TspRI CASTG 9 cut(s) 316, 426, 866, 904, 1126, 1166, 1204, 2707, 2935
Van91I CCANNNNNTGG 1 cut(s) 599
Vha464I CTTAAG 2 cut(s) 745, 1066
VpaK11BI GGWCC 1 cut(s) 1242
XbaI TCTAGA 1 cut(s) 1550
XceI RCATGY 3 cut(s) 915, 1923, 2341
XhoI CTCGAG 1 cut(s) 2433
XmiI GTMKAC 2 cut(s) 1834, 2749
XmnI GAANNNNTTC 1 cut(s) 400
XspI CTAG 9 cut(s) 117, 197, 620, 953, 1386, 1551, 1916, 2093, 2991
Zsp2I ATGCAT 3 cut(s) 913, 1925, 2635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.