Rroxscaffold_2G00089300

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000412
Physical Location & Seq
Forward (+)
11311836 .. 11322332
10497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089300.1

Sequence Viewer

Length: 5457 bp
ATGGTATCTTACATCTTCTTCTTGTATATAGTACTATTGCTTTGCTATTATGATTACCTATTTTGTTCTGCAAGAGATACACTGACACAGGCCGATCCAGTTGTTGATGAGGAATCAGGGACTCTTGTTTCAGCAGGAGAAAAATTTGAATTGGGTTTCTTTGCCCCTACGTCTATAGTTAAAAGTAACTCGAGCGCTGATAGCAGATATGTTGGCATATGGTATCATAAGGTTATTCCAATAACAGTTGTATGGGTTGCCAACAGAGATAAACCTCTACCTGGCAATTCCACTGGAGTTCTCAAAATCGAAGATGGTAACCTCCGCGTGTTGGATAAAGTTACTGGAGAGCCTTATTGGTCCACAGAGATTGGAAATTCTTCGTCTTCTAACATGATTGTGAAGATAATGGAGTCTGGGAAACCTGGTTTGACTTCTTGGAGAGACAAAGATGACCCGAGACCAGGGAACTTCAACTTCAAACTCGGTCAGAGTAAGAACCAGTATATCGTAATGAAAAATAAGTCGCTTCCTTACTGGAAAAGTGGAGACCCAGATAATCGTTTCAGCCCATATGAAGTGCCTTCTGAACTAACTCACTTGCTATCAAATTTCAGCAGCAGCACTGATACAAGTCGACCATCTATTAATTTTATAATTGGGGATTACAATTATTCACGGTTGGTGATAGATTCTACTGGAGGGTTACAGTTTTCCACTTGGAATGAAGGCAAGAAGCAGTGGTTTCTTTCATGGTGGCAGCCAAAAGATCAGTGCAGTGTGTTTAATCCTTGTGGAAAATTTGGAAGCTGTAATATTAATAGTTGGCCTTTGGTGTGCAAATGTTTGCCCGGTTTCAAGCCCAAGTCCCTAGATAATTGGAATTCTGGCGATTTTTCAGGGGGATGTGATAGAGAGTCATCAATATCTGACAACAGCACGTTCTTGAGCTTAAAGATGATGAAAGTTGGAGAGGCAGAGTCACCGTTCGATGTGGGCAGCGAAACAGAATGCAGGAAGGAGTGCCTAGAGAACCCCCAGTGCCAGGCTTACTCGTATGCCGTTCTAGCTGAAGACGGAGCTCAAAGGGGTACTACTACTACTACTACTACCAACACTTCCTCGTGCTGGACATGGTTGGAAGATTTAAATAATGTTGTAGAAGAGTATGGCAGGGGCCACAATATCTCTGTTCGAGTTGCCTCGTCTGATATAGAATCTACTGTAAGAGACTGTGAGCCTTGTGGCACAACGATGATACCCTATCCCCTGAGCACCGGAGTGGACTGTGGTGACCCCATGTACTTCCGTTTCAACTGCAACACTTTGACAGGCCAGGTTAGCTTCATGGGACCGAATGACACCTTTAGAGTTATAAGCATCAAACCAAGTATGCAAAAATTTGTACTCAAAGGGTTGCCAGTCAAAGAGGTCGATTATAAGTGTGATCCTAGAAACAGAGACGAAACTCTGCAGTTCAACACATCGTTCCCATTTAAGATAAGTAGCCCATTTAAGCTAAGTAGTTGGTGCGATGTTGACCTTGGAGGCTCTAGTTCTGAAGTATTTTCTTCTGGAGACCTTGATGTAATAGTGCTGGGTTGGGAACTACCGCTGGGAACCAGCCTGCAATACATCAGCAGACTGCAAGGGGGTTGGCCAAATTCAACCTGCAATCCAGCAAGAGATGGAATGAAAAGATGCCTTTGCAACAAAAATTTCCAATGGAATGCCAATTACTTCAATTGTACTCAAGATCAAATAAGTCGTGCACAATTTGATAGTGAAAGACGAGTCAAGGAGTTGATAGACACAAGTGAGTTCAACGAAGAAGATGAGAAAGGTATAGATGTACCCTTTTTTGATTTGCAAAGCATCCTGGAAGCTACAGATAATTTCTCAGATGCTAACAAGCTAGGACAAGGGGGATATGGGCCTGTTTACAAGGGGAAGTTTTTTGGAGGTCAAGAAATTGCAGTCAAGAGGCTATCAAAGGTTTCAGGACAAGGGTTACAAGAATTTAAAATGAGGTTGAGGATTATTCATAGAGATTTAAAAACCAGCAACGTACTTTTGGATGAGGAGATGAACCCCAAAATATCCGACTTTGGCTTGGCCAGGATTGTTGGAGGCAAAGAAACAGAGGCAAACACAAATACAGTAGTAGGAACCTATGGCTACATGTCTCCAGAGTATGCATTAGATGGAACTTTCTCAGTCAAATCAGATGTCTTTAGTTTTGGTGTGGTTCTTCTTGAGATAATCAGTGGAAAAAAGAATGCAGGATTTTTTCAGTCCAAACAAACTTTCAGCCTCCTTGGTTATGCATGGAAACTCTGGACAGAAGACAAGGTGTTGGATTTAATGGACAAGAATTTGGAAGAAAGTTGTAACAGAAGCGAATTTATAAAGTGTGTAAATGTTGGGCTCGTATGCGTTCAAGAAGATCCGGTTGATCGCCCTACAATGTCACATGTTATAACCTTGCTTGACAGTGAAACTGCAATCCCTGCAAATACAATAGCATATAACAGTTTGATTAGTGATGACAAAGGTGATACTATTGTTTCATCAGGAGAAAAATTTGAACTTGGGTTCTTTACTCCTAATGGAACCTCTGGTACTAGAAGATATGTTGGAATTTGGTATTACAGATCGAATCCACAGACTGTTGTGTGGGTTGCAAATAGAGACAACCCTCTTTCAGATACTCGTGGAGTTTTCACTATTGCAGAAGATGGAAATCTTAAGGTGTTGGATGGCAATAGAAAAACTTACTGGTCATCAAGTCTTGAAAGTTCATCATCAACCATTAGGACAGCAAAGCTCATGGACACTGGTAATCTTGTTGTGAGCAATAGAGAGCAAGGAAACAACTCGGTTCAAATTGTCTGGCAGAGTTTTGAAAATCCAACCGACACATTTCTTCCTGGCATGAAAATGAGCGGAAAATTAGTACTGAGTTCATGGAAGAGTTATAATGATCCAGCAACAGGAAATTTCACATTTCAGCAAGACCAAGAGGAAGCGAACCACTTTGTTATCTGGAAAAGATCGAGAAGGTACTGGAAGAGTGAAGATTATGGCAACTTCATTAGTTCTGATGAGATGGCTTCTGCAATTCTCTACTTGCTATCAAACTTCACCTCTACAGCAGTCCACAATGACTCTGTGCCATATCTCACCTCCTCATTATACACTTCTACAAGGCTGGTTATGAGTTTTTCGGGCCAGATTCAGTATCTGATGTGGGATAGTGAGAAGGTTTGGTCTTTGATATGGGCAGATCCTAGAGATAGATGCAGTGTGTATAATGCATGTGGAAATTTTGGTAGCTGTAACAGCAAAAATGGTTTGGTTTGCAAGTGTTTGCCTGGTTTTAAGCCTAGTTCACCGGATAATTGGAATCATGGAGATTATTCAGGCGGATGCACTAGGAAGTCAACATTATGTGGCAACAATGCTGAGACTGACACATTCTTGAGCTTAAAGATGATGAAAGTGGGAAACCCAGATTCCCAATTTAATGCCAAAAGTGAAGTGGAATGCAGAGTAGAGTGCCTTAACAACTGTGAGTGTCAAGCTTATTTCTATGAAGAGGTGGAGAACAAAAAAACGGGTGGGAGTAGTAGTTCAACATGTTGGATTTGGTCACAAGATGTCACCAATCTTCAGGAGGACTATGACGGTGGCCAGAATCTACAGGTTCGTGTAGCAGTTTCAGATATAGAATCAACAGCGAGGAGCTGTGGAAGTTGTGGCACAAACCTGATCCCTTATCCCTTAAGCACTGGACCAAAGTGTGGTGATGTCACCTACTATAGTTTCCACTGCAATATTTCAACTGGCCAGCTGAGCTTTGAGGCACCAAGCGGCACCTACCATGTCACAAGCATCAACGCGGACACACAAACATTTGTCATCCAAGCTAATGATGCAGATGAATGTAGAGATAAAAAATTTTTGAAGCTCATCCAGTCTTCTCCATATAATGTGACAAACATGTGCAATGGTGATCCGACCCGTTTTAGTCCTGACTTGTCATTTAAAGGAGGATATGAAGTTGAAGTTGCTTGGAAGTCACCCTTGGAACCACCTTGTTCCTCATCTACCGACTGCAAGGACTGGCCTCGTTCGATATGTGATGCTGCTCTAGATGGGAAGAATAGGTGTCTTTGCCCTGGAAACTCAAAATGGGATAGCAGGAGTATAAATTGTACTCAAGAAGTTGGCCACAGAAGGCAAACTGGTGAGCAAGGGAAGATGACCCTAGCTCTAATCATTGCAGTAACTTGTATAAGTGTAGCTGTTCTAGCAATTCTTTCAAGTACCTTTGTTTATGCTTATATATGGAGAAGAAGGCGCATTAAGACACAAGAAGGAAGGGCCAATCTTCAAAAGTGTTCAACACTTTATCACTTTTATGACAGTGAGAGAAAAGTCAAGAACTTGATTGAATCAGGCCGATTTAAGGATGATGATACGGAGGGCATTGATGTACCCTCTTTTGATTTGGAAAGCATACTGGTAGCTACAACATACTTCTCCAATGCAAATAAACTTGGACAAGGAGGATTTGGTCCTGTTTACAAGGGTAAACTTCCGGGAGGAGAAGAAATCGCTGTAAAGAGGCTCTCGAGTTGTTCAGGCCAAGGCTTAGAGGAATTCAAAAATGAAGTTTTGTTAATTGCCAAACTTCAACATCGGAATCTGGTTCGACTTTTGGGCTATTGTGCTGAGGGAGATGAAAAGATGTTAATCTATGAATACATGGCCAACAAAAGCTTAGACTCTTTCATCTTTGGAATTGCTCGGGGGCTTCTTTATCTTCACCAAGATTCTAGATTAAGGGTTATTCATAGAGATCTGAAAACCAGCAACATTCTACTGAGTGAAGAGATGAACCCCAAAATATCAGACTTCGGTTTGGCAAGGATCTTTGGAGGCAATGAAACTTCAGCAAACACCAATAGAGTAGTGGGAACATACGGCTATATGTCTCCAGAGTATGCATTAGATGGGTTATTCTCAGTAAAATCTGATGTTTTTAGCTTTGGTGTAGTTGTGATTGAAATCATCACTGGGAAAAGGAACACAGGATTTTATCAGCCTGAAAGATCTTTAAGTCTTCTTGGCTATGCATGGCATTTGTGGAAAGAACAAAAGGCGTTAGATTTGCTAGAACAAACACTTGGTCACAGCTGCAACAAGGATGAGTACTTCAAGTGTGTTAATGTTGGGCTCTTATGTGTACAAGAAGATCCAGGTGATCGGCCAACCATGTCACAAGTGGTTTTCATGCTCGGAAGTGAAACTGCAACAATTCCAACCCCCAAACAACCCGCTTTCGTTGTTAGGCGATGCCCTTCTAGTTCTAGCAGGGCTTCTAATTCTTCAAGCAAACCAGAAACGGTTTCGAACAATGGGTTAACTGTCACCTTAGAAGATGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin GUB_WAK_bind PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1818

Amino Acids

202.86

Weight (kDa)

5.39

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 82 - 141 1.3e-10 D-mannose binding lectin
S_locus_glycop PF00954 180 - 290 1.1e-20 S-locus glycoprotein domain
PAN_2 PF08276 310 - 385 1.9e-06 PAN-like domain
PK_Tyr_Ser-Thr PF07714 678 - 828 3.4e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 678 - 826 2.9e-22 Protein kinase domain
B_lectin PF01453 890 - 984 3.9e-30 D-mannose binding lectin
S_locus_glycop PF00954 1030 - 1130 5.7e-22 S-locus glycoprotein domain
PAN_2 PF08276 1151 - 1223 3.4e-10 PAN-like domain
GUB_WAK_bind PF13947 1251 - 1308 5.9e-06 Wall-associated receptor kinase galacturonan-binding
Pkinase PF00069 1516 - 1764 7e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1518 - 1766 2.3e-40 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 1770 - 1818 1.4e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230 AT4G03230
fragaria_vesca FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44243 FvH4_6g44244 FvH4_6g44245 FvH4_6g44245 FvH4_6g44260
malus_domestica MD04G1084700.v1.1 MD09G1099700.v1.1 MD09G1099800.v1.1 MD09G1099900.v1.1 MD09G1100000.v1.1 MD09G1100100.v1.1 MD17G1087900.v1.1
prunus_persica Prupe.3G226000_v2.0.a1 Prupe.3G226200_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226300_v2.0.a1 Prupe.3G226600_v2.0.a1 Prupe.3G226700_v2.0.a1
pyrus_communis pycom02g23070 pycom07g15320 pycom09g02310 pycom09g02330 pycom09g02340 pycom09g02350 pycom09g02360 pycom09g02400 pycom09g02410 pycom09g02430 pycom10g07020 pycom17g08490
rosa_chinensis RchiOBHm_Chr1g0328701 RchiOBHm_Chr2g0161021 RchiOBHm_Chr2g0161031 RchiOBHm_Chr2g0161081 RchiOBHm_Chr2g0161101
rosa_laevigata RLG00000021249 RLG00000021250 RLG00000021251 RLG00000021253 RLG00000021257 RLG00000029922
rosa_multiflora Rmu_sc0000955.1_g000023 Rmu_sc0000955.1_g000025 Rmu_sc0000955.1_g000041 Rmu_sc0002312.1_g000012 Rmu_sc0002481.1_g000021 Rmu_sc0002481.1_g000026 Rmu_sc0002481.1_g000028 Rmu_sc0002481.1_g000039 Rmu_sc0006420.1_g000017
rosa_roxburghii Rroxscaffold_2G00089160 Rroxscaffold_2G00089200 Rroxscaffold_2G00089220 Rroxscaffold_2G00089270 Rroxscaffold_2G00089300 Rroxscaffold_4G00321430
rosa_rugosa Rorug02G0485900 Rorug02G0486100 Rorug03G0345100
rosa_samantha Rh1AG094100 Rh1DG097200 Rh2AG552000 Rh2AG552100 Rh2AG552200 Rh2AG552300 Rh2AG552500 Rh2BG566400 Rh2BG566500 Rh2CG535800 Rh2CG535900 Rh2CG536100 Rh2CG536200 Rh2DG575000 Rh2DG575100 Rh2DG575400 Rh2DG575500 Rh2DG575600 Rh4AG077200 Rh4CG082400
rosa_wichuraiana Rw2G045690 Rw2G045700 Rw2G045710 Rw4G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 6 cut(s) 656, 1376, 1440, 2408, 2480, 2979
Acc36I ACCTGC 1 cut(s) 1679
AccB1I GGYRCC 2 cut(s) 3865, 3875
AccB7I CCANNNNNTGG 1 cut(s) 3803
AccBSI CCGCTC 1 cut(s) 2946
AccI GTMKAC 1 cut(s) 637
AccII CGCG 2 cut(s) 327, 3902
AciI CCGC 7 cut(s) 325, 1613, 2946, 3426, 3873, 3902, 5343
AclWI GGATC 9 cut(s) 89, 1442, 2441, 2978, 3281, 3766, 4010, 4943, 5255
AcoI YGGCCR 7 cut(s) 1658, 2115, 3691, 3847, 4231, 4773, 5273
AcuI CTGAAG 4 cut(s) 1092, 1581, 3656, 4941
AfeI AGCGCT 1 cut(s) 196
AfiI CCNNNNNNNGG 8 cut(s) 281, 331, 464, 1045, 1128, 2993, 3803, 4471
AflII CTTAAG 2 cut(s) 2747, 3784
AflIII ACRYGT 4 cut(s) 2181, 2473, 3638, 4002
AjuI GAANNNNNNNTTGG 6 cut(s) 2291, 2323, 4070, 4102, 5175, 5207
AleI CACNNNNGTG 1 cut(s) 1280
AloI GAACNNNNNNTCC 2 cut(s) 3616, 3648
Alw21I GWGCWC 3 cut(s) 1084, 1277, 1774
Alw44I GTGCAC 1 cut(s) 1770
AlwI GGATC 9 cut(s) 89, 1442, 2441, 2978, 3281, 3766, 4010, 4943, 5255
AlwNI CAGNNNCTG 1 cut(s) 3244
Ama87I CYCGRG 4 cut(s) 190, 457, 4636, 4812
Aor51HI AGCGCT 1 cut(s) 196
ApaLI GTGCAC 1 cut(s) 1770
ApeKI GCWGC 6 cut(s) 618, 621, 760, 999, 4148, 5202
ArsI GACNNNNNNTTYG 4 cut(s) 2214, 2246, 5179, 5211
AseI ATTAAT 2 cut(s) 648, 819
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 2 cut(s) 197, 4365
AspS9I GGNCC 8 cut(s) 360, 1177, 1352, 1934, 3229, 3794, 4386, 4580
AsuC2I CCSGG 2 cut(s) 852, 4605
AsuII TTCGAA 1 cut(s) 5417
AvaI CYCGRG 4 cut(s) 190, 457, 4636, 4812
AvaII GGWCC 4 cut(s) 360, 1352, 3794, 4580
BaeGI GKGCMC 1 cut(s) 1774
BaeI ACNNNNGTAYC 2 cut(s) 2550, 2583
BalI TGGCCA 6 cut(s) 1660, 2117, 3693, 3849, 4233, 4775
BanI GGYRCC 2 cut(s) 3865, 3875
BanII GRGCYC 3 cut(s) 1084, 2430, 5244
BauI CACGAG 2 cut(s) 1123, 2712
BbsI GAAGAC 5 cut(s) 378, 1080, 2352, 3972, 5120
Bbv12I GWGCWC 3 cut(s) 1084, 1277, 1774
BbvCI CCTCAGC 1 cut(s) 4737
BbvI GCAGC 6 cut(s) 630, 633, 772, 1011, 4135, 5189
BceAI ACGGC 2 cut(s) 1046, 5005
BcnI CCSGG 2 cut(s) 852, 4605
BfmI CTRYAG 6 cut(s) 174, 1472, 1887, 3150, 3701, 3820
BfoI RGCGCY 1 cut(s) 198
BfrI CTTAAG 2 cut(s) 2747, 3784
BfuAI ACCTGC 1 cut(s) 1679
BglII AGATCT 2 cut(s) 4864, 5117
BisI GCNGC 7 cut(s) 619, 622, 761, 1000, 3874, 4149, 5203
BlpI GCTNAGC 1 cut(s) 3854
BlsI GCNGC 7 cut(s) 620, 623, 762, 1001, 3875, 4150, 5204
BmcAI AGTACT 3 cut(s) 33, 2958, 5219
Bme18I GGWCC 4 cut(s) 360, 1352, 3794, 4580
BmeT110I CYCGRG 4 cut(s) 190, 457, 4636, 4812
BmgT120I GGNCC 8 cut(s) 360, 1177, 1352, 1934, 3229, 3794, 4386, 4580
BmiI GGNNCC 8 cut(s) 1178, 1353, 1621, 2170, 2614, 3867, 3877, 4092
BmrI ACTGGG 2 cut(s) 1033, 5091
BmuI ACTGGG 2 cut(s) 1033, 5091
BpiI GAAGAC 5 cut(s) 378, 1080, 2352, 3972, 5120
BpmI CTGGAG 6 cut(s) 315, 366, 720, 1596, 2172, 4986
Bpu10I CCTNAGC 2 cut(s) 1271, 4737
Bpu1102I GCTNAGC 1 cut(s) 3854
Bpu14I TTCGAA 1 cut(s) 5417
BpuEI CTTGAG 5 cut(s) 967, 1737, 2276, 3502, 4206
BpuMI CCSGG 2 cut(s) 852, 4605
BsaBI GATNNNNATC 3 cut(s) 2742, 4757, 5072
BsaI GGTCTC 3 cut(s) 454, 543, 1572
BsaJI CCNNGG 6 cut(s) 464, 1543, 2317, 4086, 4180, 4651
BsaWI WCCGGW 3 cut(s) 1277, 2449, 3394
BsaXI ACNNNNNCTCC 2 cut(s) 3616, 3646
Bsc4I CCNNNNNNNGG 8 cut(s) 281, 331, 464, 1045, 1128, 2993, 3803, 4471
Bse3DI GCAATG 3 cut(s) 4015, 4281, 4954
Bse8I GATNNNNATC 3 cut(s) 2742, 4757, 5072
BseDI CCNNGG 6 cut(s) 464, 1543, 2317, 4086, 4180, 4651
BseGI GGATG 9 cut(s) 911, 1875, 2083, 2764, 3434, 3921, 3972, 4480, 5218
BseJI GATNNNNATC 3 cut(s) 2742, 4757, 5072
BseLI CCNNNNNNNGG 8 cut(s) 281, 331, 464, 1045, 1128, 2993, 3803, 4471
BseMI GCAATG 3 cut(s) 4015, 4281, 4954
BseMII CTCAG 9 cut(s) 1262, 1914, 2229, 2951, 3456, 3845, 4728, 4880, 5043
BseRI GAGGAG 4 cut(s) 2096, 3178, 3757, 4623
BseSI GKGCMC 1 cut(s) 1774
BseXI GCAGC 6 cut(s) 630, 633, 772, 1011, 4135, 5189
BseYI CCCAGC 2 cut(s) 1597, 1615
BsgI GTGCAG 1 cut(s) 796
Bsh1236I CGCG 2 cut(s) 327, 3902
BshNI GGYRCC 2 cut(s) 3865, 3875
BsiHKAI GWGCWC 3 cut(s) 1084, 1277, 1774
BsiHKCI CYCGRG 4 cut(s) 190, 457, 4636, 4812
BsiSI CCGG 5 cut(s) 852, 1278, 2450, 3395, 4604
BslFI GGGAC 3 cut(s) 133, 853, 1365
BslI CCNNNNNNNGG 8 cut(s) 281, 331, 464, 1045, 1128, 2993, 3803, 4471
BsmBI CGTCTC 1 cut(s) 1455
BsmFI GGGAC 3 cut(s) 133, 853, 1365
BsmI GAATGC 4 cut(s) 1016, 1735, 2284, 3551
Bso31I GGTCTC 3 cut(s) 454, 543, 1572
BsoBI CYCGRG 4 cut(s) 190, 457, 4636, 4812
Bsp119I TTCGAA 1 cut(s) 5417
Bsp1286I GDGCHC 5 cut(s) 1084, 1277, 1774, 2430, 5244
Bsp1407I TGTACA 1 cut(s) 5251
Bsp1720I GCTNAGC 1 cut(s) 3854
BspACI CCGC 7 cut(s) 325, 1613, 2946, 3426, 3873, 3902, 5343
BspCNI CTCAG 9 cut(s) 1263, 1913, 2228, 2952, 3457, 3846, 4729, 4881, 5042
BspFNI CGCG 2 cut(s) 327, 3902
BspLI GGNNCC 8 cut(s) 1178, 1353, 1621, 2170, 2614, 3867, 3877, 4092
BspMAI CTGCAG 1 cut(s) 1476
BspMI ACCTGC 1 cut(s) 1679
BspPI GGATC 9 cut(s) 89, 1442, 2441, 2978, 3281, 3766, 4010, 4943, 5255
BspT104I TTCGAA 1 cut(s) 5417
BspT107I GGYRCC 2 cut(s) 3865, 3875
BspTI CTTAAG 2 cut(s) 2747, 3784
BspTNI GGTCTC 3 cut(s) 454, 543, 1572
BsrBI CCGCTC 1 cut(s) 2946
BsrDI GCAATG 3 cut(s) 4015, 4281, 4954
BsrGI TGTACA 1 cut(s) 5251
BssECI CCNNGG 6 cut(s) 464, 1543, 2317, 4086, 4180, 4651
BssSI CACGAG 2 cut(s) 1123, 2712
BssT1I CCWWGG 4 cut(s) 1543, 2317, 4086, 4651
Bst2BI CACGAG 2 cut(s) 1123, 2712
Bst6I CTCTTC 5 cut(s) 1158, 2966, 3065, 3591, 4890
BstAFI CTTAAG 2 cut(s) 2747, 3784
BstAPI GCANNNNNTGC 1 cut(s) 2510
BstAUI TGTACA 1 cut(s) 5251
BstBI TTCGAA 1 cut(s) 5417
BstC8I GCNNGC 2 cut(s) 1628, 3851
BstEII GGTNACC 2 cut(s) 317, 1292
BstF5I GGATG 9 cut(s) 911, 1875, 2083, 2764, 3434, 3921, 3972, 4480, 5218
BstFNI CGCG 2 cut(s) 327, 3902
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 2 cut(s) 197, 4365
BstMWI GCNNNNNNNGC 8 cut(s) 201, 1067, 1341, 2510, 3342, 3855, 3935, 4313
BstNSI RCATGY 5 cut(s) 2185, 2477, 3321, 3642, 4006
BstPI GGTNACC 2 cut(s) 317, 1292
BstSFI CTRYAG 6 cut(s) 174, 1472, 1887, 3150, 3701, 3820
BstSLI GKGCMC 1 cut(s) 1774
BstUI CGCG 2 cut(s) 327, 3902
BstV1I GCAGC 6 cut(s) 630, 633, 772, 1011, 4135, 5189
BstV2I GAAGAC 5 cut(s) 378, 1080, 2352, 3972, 5120
BstX2I RGATCY 6 cut(s) 2446, 3286, 4864, 4935, 5117, 5260
BstYI RGATCY 6 cut(s) 2446, 3286, 4864, 4935, 5117, 5260
BtgZI GCGATG 2 cut(s) 1548, 5374
BtsCI GGATG 9 cut(s) 911, 1875, 2083, 2764, 3434, 3921, 3972, 4480, 5218
BtsI GCAGTG 4 cut(s) 746, 784, 3310, 3829
BveI ACCTGC 1 cut(s) 1679
Cac8I GCNNGC 2 cut(s) 1628, 3851
CaiI CAGNNNCTG 1 cut(s) 3244
CfoI GCGC 2 cut(s) 197, 4365
Cfr13I GGNCC 8 cut(s) 360, 1177, 1352, 1934, 3229, 3794, 4386, 4580
CsiI ACCWGGT 1 cut(s) 424
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
DraI TTTAAA 4 cut(s) 1149, 2023, 2055, 4048
EaeI YGGCCR 7 cut(s) 1658, 2115, 3691, 3847, 4231, 4773, 5273
Eam1104I CTCTTC 5 cut(s) 1158, 2966, 3065, 3591, 4890
EarI CTCTTC 5 cut(s) 1158, 2966, 3065, 3591, 4890
EciI GGCGGA 1 cut(s) 3441
Ecl136II GAGCTC 1 cut(s) 1082
Eco130I CCWWGG 4 cut(s) 1543, 2317, 4086, 4651
Eco24I GRGCYC 3 cut(s) 1084, 2430, 5244
Eco31I GGTCTC 3 cut(s) 454, 543, 1572
Eco47I GGWCC 4 cut(s) 360, 1352, 3794, 4580
Eco47III AGCGCT 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 1082
Eco57I CTGAAG 4 cut(s) 1092, 1581, 3656, 4941
Eco88I CYCGRG 4 cut(s) 190, 457, 4636, 4812
Eco91I GGTNACC 2 cut(s) 317, 1292
EcoICRI GAGCTC 1 cut(s) 1082
EcoO65I GGTNACC 2 cut(s) 317, 1292
EcoRI GAATTC 2 cut(s) 883, 4664
EcoT14I CCWWGG 4 cut(s) 1543, 2317, 4086, 4651
EcoT22I ATGCAT 5 cut(s) 2200, 2329, 3319, 5014, 5143
EcoT38I GRGCYC 3 cut(s) 1084, 2430, 5244
ErhI CCWWGG 4 cut(s) 1543, 2317, 4086, 4651
Esp3I CGTCTC 1 cut(s) 1455
FalI AAGNNNNNCTT 2 cut(s) 4070, 4102
FaqI GGGAC 3 cut(s) 133, 853, 1365
FauI CCCGC 1 cut(s) 5350
FauNDI CATATG 2 cut(s) 218, 574
FblI GTMKAC 1 cut(s) 637
Fnu4HI GCNGC 7 cut(s) 619, 622, 761, 1000, 3874, 4149, 5203
FokI GGATG 9 cut(s) 918, 1862, 2090, 2771, 3441, 3908, 3959, 4487, 5225
FriOI GRGCYC 3 cut(s) 1084, 2430, 5244
Fsp4HI GCNGC 7 cut(s) 619, 622, 761, 1000, 3874, 4149, 5203
GlaI GCGC 2 cut(s) 196, 4364
GluI GCNGC 7 cut(s) 619, 622, 761, 1000, 3874, 4149, 5203
GsaI CCCAGC 2 cut(s) 1601, 1619
GsuI CTGGAG 6 cut(s) 315, 366, 720, 1596, 2172, 4986
HaeII RGCGCY 1 cut(s) 198
HapII CCGG 5 cut(s) 852, 1278, 2450, 3395, 4604
HhaI GCGC 2 cut(s) 197, 4365
Hin6I GCGC 2 cut(s) 195, 4363
HinP1I GCGC 2 cut(s) 195, 4363
HincII GTYRAC 4 cut(s) 638, 1540, 3444, 5430
HindII GTYRAC 4 cut(s) 638, 1540, 3444, 5430
HindIII AAGCTT 2 cut(s) 3582, 4783
HpaI GTTAAC 1 cut(s) 5430
HpaII CCGG 5 cut(s) 852, 1278, 2450, 3395, 4604
HpyCH4IV ACGT 3 cut(s) 170, 941, 2067
HpyF10VI GCNNNNNNNGC 8 cut(s) 201, 1067, 1341, 2510, 3342, 3855, 3935, 4313
HpySE526I ACGT 3 cut(s) 170, 941, 2067
HspAI GCGC 2 cut(s) 195, 4363
KspAI GTTAAC 1 cut(s) 5430
LmnI GCTCC 2 cut(s) 1079, 3744
Lsp1109I GCAGC 6 cut(s) 630, 633, 772, 1011, 4135, 5189
MabI ACCWGGT 1 cut(s) 424
MaeII ACGT 3 cut(s) 170, 941, 2067
MbiI CCGCTC 1 cut(s) 2946
MfeI CAATTG 1 cut(s) 1744
MflI RGATCY 6 cut(s) 2446, 3286, 4864, 4935, 5117, 5260
MhlI GDGCHC 5 cut(s) 1084, 1277, 1774, 2430, 5244
MlsI TGGCCA 6 cut(s) 1660, 2117, 3693, 3849, 4233, 4775
MluNI TGGCCA 6 cut(s) 1660, 2117, 3693, 3849, 4233, 4775
MlyI GAGTC 7 cut(s) 115, 422, 926, 989, 1803, 3161, 4784
Mox20I TGGCCA 6 cut(s) 1660, 2117, 3693, 3849, 4233, 4775
Mph1103I ATGCAT 5 cut(s) 2200, 2329, 3319, 5014, 5143
MroXI GAANNNNTTC 1 cut(s) 379
MscI TGGCCA 6 cut(s) 1660, 2117, 3693, 3849, 4233, 4775
MslI CAYNNNNRTG 5 cut(s) 398, 1253, 1280, 2939, 4422
Msp20I TGGCCA 6 cut(s) 1660, 2117, 3693, 3849, 4233, 4775
MspA1I CMGCKG 3 cut(s) 1615, 3853, 5202
MspCI CTTAAG 2 cut(s) 2747, 3784
MspI CCGG 5 cut(s) 852, 1278, 2450, 3395, 4604
MunI CAATTG 1 cut(s) 1744
Mva1269I GAATGC 4 cut(s) 1016, 1735, 2284, 3551
MvnI CGCG 2 cut(s) 327, 3902
MwoI GCNNNNNNNGC 8 cut(s) 201, 1067, 1341, 2510, 3342, 3855, 3935, 4313
NciI CCSGG 2 cut(s) 852, 4605
NdeI CATATG 2 cut(s) 218, 574
NlaIV GGNNCC 8 cut(s) 1178, 1353, 1621, 2170, 2614, 3867, 3877, 4092
NsiI ATGCAT 5 cut(s) 2200, 2329, 3319, 5014, 5143
NspI RCATGY 5 cut(s) 2185, 2477, 3321, 3642, 4006
NspV TTCGAA 1 cut(s) 5417
OliI CACNNNNGTG 1 cut(s) 1280
PaeR7I CTCGAG 2 cut(s) 190, 4636
PciI ACATGT 4 cut(s) 2181, 2473, 3638, 4002
PctI GAATGC 4 cut(s) 1016, 1735, 2284, 3551
PdmI GAANNNNTTC 1 cut(s) 379
PflMI CCANNNNNTGG 1 cut(s) 3803
PfoI TCCNGGA 2 cut(s) 1878, 4603
PkrI GCNGC 7 cut(s) 620, 623, 762, 1001, 3875, 4150, 5204
PleI GAGTC 7 cut(s) 115, 421, 925, 988, 1802, 3161, 4784
PpsI GAGTC 7 cut(s) 115, 421, 925, 988, 1802, 3161, 4784
PscI ACATGT 4 cut(s) 2181, 2473, 3638, 4002
PshBI ATTAAT 2 cut(s) 648, 819
PsiI TTATAA 6 cut(s) 656, 1376, 1440, 2408, 2480, 2979
Psp124BI GAGCTC 1 cut(s) 1084
PspEI GGTNACC 2 cut(s) 317, 1292
PspFI CCCAGC 2 cut(s) 1597, 1615
PspN4I GGNNCC 8 cut(s) 1178, 1353, 1621, 2170, 2614, 3867, 3877, 4092
PspPI GGNCC 8 cut(s) 360, 1177, 1352, 1934, 3229, 3794, 4386, 4580
PspXI VCTCGAGB 1 cut(s) 190
PsrI GAACNNNNNNTAC 2 cut(s) 2605, 2637
PstI CTGCAG 1 cut(s) 1476
PstNI CAGNNNCTG 1 cut(s) 3244
PsuI RGATCY 6 cut(s) 2446, 3286, 4864, 4935, 5117, 5260
PvuII CAGCTG 2 cut(s) 3853, 5202
RseI CAYNNNNRTG 5 cut(s) 398, 1253, 1280, 2939, 4422
SacI GAGCTC 1 cut(s) 1084
SalI GTCGAC 1 cut(s) 636
SatI GCNGC 7 cut(s) 619, 622, 761, 1000, 3874, 4149, 5203
Sau96I GGNCC 8 cut(s) 360, 1177, 1352, 1934, 3229, 3794, 4386, 4580
ScaI AGTACT 3 cut(s) 33, 2958, 5219
SchI GAGTC 7 cut(s) 115, 422, 926, 989, 1803, 3161, 4784
SduI GDGCHC 5 cut(s) 1084, 1277, 1774, 2430, 5244
SexAI ACCWGGT 1 cut(s) 424
SfcI CTRYAG 6 cut(s) 174, 1472, 1887, 3150, 3701, 3820
Sfr274I CTCGAG 2 cut(s) 190, 4636
SfuI TTCGAA 1 cut(s) 5417
SinI GGWCC 4 cut(s) 360, 1352, 3794, 4580
SlaI CTCGAG 2 cut(s) 190, 4636
SmiI ATTTAAAT 1 cut(s) 1149
SmiMI CAYNNNNRTG 5 cut(s) 398, 1253, 1280, 2939, 4422
SmlI CTYRAG 9 cut(s) 190, 946, 1752, 2255, 2747, 3481, 3784, 4221, 4636
SmoI CTYRAG 9 cut(s) 190, 946, 1752, 2255, 2747, 3481, 3784, 4221, 4636
SsiI CCGC 7 cut(s) 325, 1613, 2946, 3426, 3873, 3902, 5343
SspI AATATT 2 cut(s) 817, 3838
SstI GAGCTC 1 cut(s) 1084
StyI CCWWGG 4 cut(s) 1543, 2317, 4086, 4651
SwaI ATTTAAAT 1 cut(s) 1149
TaiI ACGT 3 cut(s) 173, 944, 2070
TaqII GACCGA 2 cut(s) 476, 1369
TatI WGTACW 8 cut(s) 31, 1302, 1405, 1748, 2956, 4217, 5217, 5251
TauI GCSGC 1 cut(s) 3876
TseI GCWGC 6 cut(s) 618, 621, 760, 999, 4148, 5202
TspGWI ACGGA 3 cut(s) 1092, 1298, 4499
Van91I CCANNNNNTGG 1 cut(s) 3803
Vha464I CTTAAG 2 cut(s) 2747, 3784
VneI GTGCAC 1 cut(s) 1770
VpaK11BI GGWCC 4 cut(s) 360, 1352, 3794, 4580
VspI ATTAAT 2 cut(s) 648, 819
XbaI TCTAGA 2 cut(s) 4153, 4841
XceI RCATGY 5 cut(s) 2185, 2477, 3321, 3642, 4006
XcmI CCANNNNNNNNNTGG 3 cut(s) 3538, 4975, 5287
XhoI CTCGAG 2 cut(s) 190, 4636
XmiI GTMKAC 1 cut(s) 637
XmnI GAANNNNTTC 1 cut(s) 379
ZrmI AGTACT 3 cut(s) 33, 2958, 5219
Zsp2I ATGCAT 5 cut(s) 2200, 2329, 3319, 5014, 5143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.