MD14G1160300.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000392
Physical Location & Seq
Reverse (-)
25409475 .. 25411723
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1160300.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGATGGGGGCTCATGTTGATGAAACCTATGCAGAGGGAAGCATAAGTGGTGGAGTTAACCAACTTGGTGTAGATCATTACAATCGTCTGATCAACGAATTAATAAGAAATGGCATTGAGCCTTTTGTCACGATCTATCACTTTGACTTGCCCCAAGTTCTGCAAGAGAAGTATGGAGGCTTGATGAATCACATGTTTGTGAAAGACTTCAGCGATTATGCTGAGCTCTGTTTCAAATTGTTTGGTGATAGAGTTAGGCATTGGTTCACCATTAATGAGCCAAATGTTCTTGCACAATATGGCTACGAGATTGGGATTGCCCCACCAGGAAGGTGCTCGCTTCCACAAGGACGTTGTGGGTTGGGATCTCCACCTCGATGTATGGTGCCCGTCGGTCCCTGTAACTTTGGTGGTAACTCGTCCACGGAGCCTTACATTGCTGCCCATCACATCATCCTTGCTCACGCCACTGCTGTGAAGCTATATAAAGAGAGATACCAGGTTAAACTTATAGAAACCACGTTCATCTCTATTCAAAGTTCAAAACTCATTGAAGGAATCTTGATAAAGTTTGTTTTGAATGTCGTCTTGCAGGTAGAGCAAAATGGAGAGATCGGAATCGTCCTTGCTACTAAGTATTTTAAGCCATATTCTAATTCACAGGAAGATCAAGCTGCAGCAAAGAGACTGTTTGATTTTTATCTAGGGTGGTTTATGGGACCGTTGGTATTTGGAAAATACCCACAAAGCATGAAAGAGCTGGTGAAGGAAAGGCTGCCCACATTTTCCGCTGAAGAGAAATCTCTTGTCAAGGGGAATTTAGGCTTTGTTGGGATAAACTATTATGAATCAGTTCATGCGAAATATAGGCCTCCTCCTCCTGCAGAGAATCTGAGCTATTCCTTCGATTCATGGGCGGAAGAAAAAGATATGAAAGATGTTCCTGGTGTGTCCGAGAACCATTGGCCACAAGGGCTGCAGAAGCTCATGAACTATGTGAAGAATGAATATCGTAGCCCACAAATCTACATTTCCGAAAATGGATATGGTAATCCAAGAAATGACAGACTTCCGCTCGATGAACAAGCAAAAGACCCAAATCGAATCTCCTATATCGCTCGGCATTTGTATTGGCTCAATAAGGCAATGAAGAATGGAGCAAATGTCAAAGGGTACTTTTGTTGGGCTCTGTTCGATGACTTTGAATGGGGTATAGGGTTGTCTCAACAAGTTGGACTCTATTACGTTGATTTTGATGACAACTACAAGCGCTACCCCAAGCAATCAGCTAAGTGGTTCCGTGATTTCAACCACAACAGTGCTGCAATTAGTAGATCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

448

Amino Acids

51.12

Weight (kDa)

6.87

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 16 - 173 3.7e-41 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 197 - 439 6.9e-59 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25630 AT2G44450 AT2G44450 AT3G60130 AT3G60130 AT3G60130 AT5G42260 AT5G44640
fragaria_vesca FvH4_5g07160 FvH4_5g07180 FvH4_5g07811 FvH4_5g07831 FvH4_5g07831 FvH4_5g07832 FvH4_6g19950 FvH4_6g19950 FvH4_6g19950
malus_domestica MD01G1120900.v1.1 MD06G1144600.v1.1 MD06G1144800.v1.1 MD06G1145700.v1.1 MD06G1146100.v1.1 MD06G1147000.v1.1 MD08G1072600.v1.1 MD08G1142300.v1.1 MD08G1191100.v1.1 MD14G1159400.v1.1 MD14G1160300.v1.1 MD14G1160600.v1.1 MD14G1160700.v1.1 MD14G1161400.v1.1
prunus_persica Prupe.1G525400_v2.0.a1 Prupe.1G583900_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G156500_v2.0.a1
pyrus_communis pycom06g13390 pycom06g13520 pycom06g13560 pycom06g13640 pycom08g12030 pycom14g13310 pycom14g13430
rosa_chinensis RchiOBHm_Chr3g0475401 RchiOBHm_Chr7g0190551 RchiOBHm_Chr7g0190631 RchiOBHm_Chr7g0190641 RchiOBHm_Chr7g0191241 RchiOBHm_Chr7g0191251 RchiOBHm_Chr7g0191261 RchiOBHm_Chr7g0202591
rosa_laevigata RLG00000004507 RLG00000004508 RLG00000004516 RLG00000023846 RLG00000035079
rosa_multiflora Rmu_co8005672.1_g000001 Rmu_co8018538.1_g000001 Rmu_sc0000526.1_g000002 Rmu_sc0001786.1_g000011 Rmu_sc0006709.1_g000007 Rmu_sc0006709.1_g000008 Rmu_sc0016637.1_g000004
rosa_roxburghii Rroxscaffold_1G00018560 Rroxscaffold_1G00024050 Rroxscaffold_3G00264440 Rroxscaffold_3G00264450 Rroxscaffold_4G00306640 Rroxscaffold_6G00406340
rosa_rugosa Rorug03G0295600 Rorug05G0298500 Rorug06G0072900 Rorug06G0505100 Rorug06G0505800 Rorug06G0505900 Rorug06G0506000
rosa_samantha Rh1CG344200 Rh2AG107900 Rh3AG198700 Rh3CG223000 Rh5AG369500 Rh5BG469700 Rh5BG469800 Rh5DG483000 Rh7AG111700 Rh7AG112400 Rh7AG112500 Rh7AG112600 Rh7CG116400 Rh7CG117400 Rh7CG117500 Rh7CG117600 Rh7CG122100 Rh7CG122200 Rh7DG115600 Rh7DG116400 Rh7DG116500
rosa_wichuraiana Rw3G018030 Rw7G009740 Rw7G009810 Rw7G009820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 385
AccBSI CCGCTC 1 cut(s) 1075
AciI CCGC 3 cut(s) 789, 917, 1073
AclWI GGATC 1 cut(s) 373
AcoI YGGCCR 1 cut(s) 965
AcsI RAATTY 1 cut(s) 817
AcuI CTGAAG 2 cut(s) 193, 813
AfaI GTAC 1 cut(s) 1175
AfeI AGCGCT 1 cut(s) 1271
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 7 cut(s) 235, 536, 543, 554, 580, 1205, 1309
AjnI CCWGG 3 cut(s) 325, 498, 943
AleI CACNNNNGTG 2 cut(s) 473, 1317
AluBI AGCT 7 cut(s) 226, 481, 674, 760, 897, 985, 1289
AluI AGCT 7 cut(s) 226, 481, 674, 760, 897, 985, 1289
Alw21I GWGCWC 2 cut(s) 228, 338
Alw26I GTCTC 2 cut(s) 679, 1227
AlwI GGATC 1 cut(s) 373
Aor51HI AGCGCT 1 cut(s) 1271
AoxI GGCC 2 cut(s) 869, 965
ApeKI GCWGC 6 cut(s) 440, 674, 677, 775, 976, 1322
ApoI RAATTY 1 cut(s) 817
AseI ATTAAT 2 cut(s) 101, 273
Asp700I GAANNNNTTC 2 cut(s) 206, 852
AspLEI GCGC 1 cut(s) 1272
AspS9I GGNCC 2 cut(s) 395, 719
AsuHPI GGTGA 3 cut(s) 257, 259, 775
AvaII GGWCC 2 cut(s) 395, 719
BaeGI GKGCMC 1 cut(s) 390
BaeI ACNNNNGTAYC 2 cut(s) 1165, 1198
BalI TGGCCA 1 cut(s) 967
BanI GGYRCC 1 cut(s) 385
BanII GRGCYC 3 cut(s) 13, 228, 1189
Bbv12I GWGCWC 2 cut(s) 228, 338
BbvI GCAGC 6 cut(s) 427, 661, 689, 762, 963, 1309
BccI CCATC 1 cut(s) 453
BciT130I CCWGG 3 cut(s) 327, 500, 945
BclI TGATCA 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 679, 1227
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 3 cut(s) 675, 882, 977
BfoI RGCGCY 1 cut(s) 1273
BfuAI ACCTGC 1 cut(s) 583
BglI GCCNNNNNGGC 1 cut(s) 973
BisI GCNGC 6 cut(s) 441, 675, 678, 776, 977, 1323
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 6 cut(s) 442, 676, 679, 777, 978, 1324
Bme1390I CCNGG 3 cut(s) 327, 500, 945
Bme18I GGWCC 2 cut(s) 395, 719
BmgT120I GGNCC 2 cut(s) 395, 719
BmiI GGNNCC 5 cut(s) 387, 397, 429, 720, 1298
BmrFI CCNGG 3 cut(s) 327, 500, 945
Bpu1102I GCTNAGC 1 cut(s) 222
BsaBI GATNNNNATC 2 cut(s) 617, 699
BsaJI CCNNGG 1 cut(s) 423
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bse3DI GCAATG 2 cut(s) 435, 1152
Bse8I GATNNNNATC 2 cut(s) 617, 699
BseBI CCWGG 3 cut(s) 327, 500, 945
BseDI CCNNGG 1 cut(s) 423
BseGI GGATG 1 cut(s) 453
BseJI GATNNNNATC 2 cut(s) 617, 699
BseMI GCAATG 2 cut(s) 435, 1152
BseMII CTCAG 2 cut(s) 213, 884
BseRI GAGGAG 2 cut(s) 864, 867
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 6 cut(s) 427, 661, 689, 762, 963, 1309
BshFI GGCC 2 cut(s) 871, 967
BshNI GGYRCC 1 cut(s) 385
BsiHKAI GWGCWC 2 cut(s) 228, 338
BslFI GGGAC 2 cut(s) 381, 732
BsmAI GTCTC 2 cut(s) 679, 1227
BsmFI GGGAC 2 cut(s) 381, 732
BsnI GGCC 2 cut(s) 871, 967
Bsp1286I GDGCHC 5 cut(s) 13, 228, 338, 390, 1189
Bsp143I GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
Bsp1720I GCTNAGC 1 cut(s) 222
BspACI CCGC 3 cut(s) 789, 917, 1073
BspANI GGCC 2 cut(s) 871, 967
BspCNI CTCAG 2 cut(s) 214, 885
BspHI TCATGA 1 cut(s) 987
BspLI GGNNCC 5 cut(s) 387, 397, 429, 720, 1298
BspMAI CTGCAG 3 cut(s) 679, 886, 981
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 385
BsrBI CCGCTC 1 cut(s) 1075
BsrDI GCAATG 2 cut(s) 435, 1152
BssECI CCNNGG 1 cut(s) 423
BssMI GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
Bst2UI CCWGG 3 cut(s) 327, 500, 945
Bst4CI ACNGT 3 cut(s) 690, 723, 1319
Bst6I CTCTTC 1 cut(s) 789
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 5 cut(s) 222, 633, 893, 1290, 1341
BstDSI CCRYGG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 453
BstH2I RGCGCY 1 cut(s) 1273
BstHHI GCGC 1 cut(s) 1272
BstKTI GATC 7 cut(s) 76, 93, 135, 368, 615, 670, 1337
BstMAI GTCTC 2 cut(s) 679, 1227
BstMBI GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
BstMWI GCNNNNNNNGC 3 cut(s) 598, 973, 982
BstNI CCWGG 3 cut(s) 327, 500, 945
BstNSI RCATGY 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 325, 498, 943
BstSFI CTRYAG 3 cut(s) 675, 882, 977
BstSLI GKGCMC 1 cut(s) 390
BstV1I GCAGC 6 cut(s) 427, 661, 689, 762, 963, 1309
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuRI GGCC 2 cut(s) 871, 967
BtgI CCRYGG 1 cut(s) 423
BtsCI GGATG 1 cut(s) 453
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 468, 1324
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 338
CciI TCATGA 1 cut(s) 987
CfoI GCGC 1 cut(s) 1272
Cfr13I GGNCC 2 cut(s) 395, 719
CsiI ACCWGGT 1 cut(s) 498
Csp6I GTAC 1 cut(s) 1174
CviAII CATG 6 cut(s) 14, 193, 751, 857, 912, 988
CviQI GTAC 1 cut(s) 1174
DdeI CTNAG 5 cut(s) 222, 633, 893, 1290, 1341
DpnI GATC 7 cut(s) 75, 92, 134, 367, 614, 669, 1336
DpnII GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
EaeI YGGCCR 1 cut(s) 965
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
EciI GGCGGA 1 cut(s) 932
Ecl136II GAGCTC 1 cut(s) 226
Eco147I AGGCCT 1 cut(s) 871
Eco24I GRGCYC 3 cut(s) 13, 228, 1189
Eco47I GGWCC 2 cut(s) 395, 719
Eco47III AGCGCT 1 cut(s) 1271
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 193, 813
EcoICRI GAGCTC 1 cut(s) 226
EcoRII CCWGG 3 cut(s) 325, 498, 943
EcoT38I GRGCYC 3 cut(s) 13, 228, 1189
FaeI CATG 6 cut(s) 17, 196, 754, 860, 915, 991
FaqI GGGAC 2 cut(s) 381, 732
FatI CATG 6 cut(s) 13, 192, 750, 856, 911, 987
FbaI TGATCA 1 cut(s) 90
Fnu4HI GCNGC 6 cut(s) 441, 675, 678, 776, 977, 1323
FokI GGATG 1 cut(s) 440
FriOI GRGCYC 3 cut(s) 13, 228, 1189
Fsp4HI GCNGC 6 cut(s) 441, 675, 678, 776, 977, 1323
FspBI CTAG 1 cut(s) 704
GlaI GCGC 1 cut(s) 1271
GluI GCNGC 6 cut(s) 441, 675, 678, 776, 977, 1323
HaeII RGCGCY 1 cut(s) 1273
HaeIII GGCC 2 cut(s) 871, 967
HhaI GCGC 1 cut(s) 1272
Hin1II CATG 6 cut(s) 17, 196, 754, 860, 915, 991
Hin6I GCGC 1 cut(s) 1270
HinP1I GCGC 1 cut(s) 1270
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 8 cut(s) 187, 558, 618, 848, 889, 908, 1104, 1236
HpaI GTTAAC 1 cut(s) 58
HphI GGTGA 3 cut(s) 257, 259, 775
Hpy166II GTNNAC 3 cut(s) 58, 267, 423
Hpy188I TCNGA 5 cut(s) 90, 617, 894, 955, 1036
Hpy188III TCNNGA 3 cut(s) 130, 562, 988
Hpy8I GTNNAC 3 cut(s) 58, 267, 423
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 4 cut(s) 324, 548, 760, 913
HpyCH4III ACNGT 3 cut(s) 690, 723, 1319
HpyCH4IV ACGT 3 cut(s) 352, 521, 1245
HpyCH4V TGCA 8 cut(s) 32, 163, 293, 592, 677, 884, 979, 1325
HpyF10VI GCNNNNNNNGC 3 cut(s) 598, 973, 982
HpyF3I CTNAG 5 cut(s) 222, 633, 893, 1290, 1341
HpySE526I ACGT 3 cut(s) 352, 521, 1245
Hsp92II CATG 6 cut(s) 17, 196, 754, 860, 915, 991
HspAI GCGC 1 cut(s) 1270
Ksp22I TGATCA 1 cut(s) 90
KspAI GTTAAC 1 cut(s) 58
Kzo9I GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
LmnI GCTCC 2 cut(s) 427, 1157
Lsp1109I GCAGC 6 cut(s) 427, 661, 689, 762, 963, 1309
MabI ACCWGGT 1 cut(s) 498
MaeI CTAG 1 cut(s) 704
MaeII ACGT 3 cut(s) 352, 521, 1245
MaeIII GTNAC 3 cut(s) 127, 401, 413
MalI GATC 7 cut(s) 75, 92, 134, 367, 614, 669, 1336
MbiI CCGCTC 1 cut(s) 1075
MboI GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
MboII GAAGA 5 cut(s) 677, 806, 932, 1012, 1162
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 5 cut(s) 13, 228, 338, 390, 1189
MlsI TGGCCA 1 cut(s) 967
MluCI AATT 5 cut(s) 98, 236, 655, 817, 1326
MluNI TGGCCA 1 cut(s) 967
MlyI GAGTC 1 cut(s) 1230
MmeI TCCRAC 1 cut(s) 1213
MnlI CCTC 6 cut(s) 28, 170, 384, 882, 885, 888
Mox20I TGGCCA 1 cut(s) 967
MroXI GAANNNNTTC 2 cut(s) 206, 852
MscI TGGCCA 1 cut(s) 967
MseI TTAA 5 cut(s) 57, 101, 273, 504, 642
MslI CAYNNNNRTG 5 cut(s) 18, 197, 376, 473, 1317
Msp20I TGGCCA 1 cut(s) 967
MspA1I CMGCKG 1 cut(s) 791
MspR9I CCNGG 3 cut(s) 327, 500, 945
MvaI CCWGG 3 cut(s) 327, 500, 945
MwoI GCNNNNNNNGC 3 cut(s) 598, 973, 982
NdeII GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
NlaIII CATG 6 cut(s) 17, 196, 754, 860, 915, 991
NlaIV GGNNCC 5 cut(s) 387, 397, 429, 720, 1298
NmeAIII GCCGAG 1 cut(s) 1099
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 196
OliI CACNNNNGTG 2 cut(s) 473, 1317
PagI TCATGA 1 cut(s) 987
PceI AGGCCT 1 cut(s) 871
PciI ACATGT 1 cut(s) 192
PdmI GAANNNNTTC 2 cut(s) 206, 852
PfeI GAWTC 7 cut(s) 187, 558, 618, 848, 889, 908, 1104
PkrI GCNGC 6 cut(s) 442, 676, 679, 777, 978, 1324
PleI GAGTC 1 cut(s) 1230
PpsI GAGTC 1 cut(s) 1230
PscI ACATGT 1 cut(s) 192
PshBI ATTAAT 2 cut(s) 101, 273
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 325, 498, 943
PspGI CCWGG 3 cut(s) 325, 498, 943
PspN4I GGNNCC 5 cut(s) 387, 397, 429, 720, 1298
PspPI GGNCC 2 cut(s) 395, 719
PstI CTGCAG 3 cut(s) 679, 886, 981
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 1 cut(s) 1175
RsaNI GTAC 1 cut(s) 1174
RseI CAYNNNNRTG 5 cut(s) 18, 197, 376, 473, 1317
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 5 cut(s) 57, 101, 273, 504, 642
SatI GCNGC 6 cut(s) 441, 675, 678, 776, 977, 1323
Sau3AI GATC 7 cut(s) 73, 90, 132, 365, 612, 667, 1334
Sau96I GGNCC 2 cut(s) 395, 719
SchI GAGTC 1 cut(s) 1230
ScrFI CCNGG 3 cut(s) 327, 500, 945
SduI GDGCHC 5 cut(s) 13, 228, 338, 390, 1189
SexAI ACCWGGT 1 cut(s) 498
SfcI CTRYAG 3 cut(s) 675, 882, 977
SinI GGWCC 2 cut(s) 395, 719
SmiMI CAYNNNNRTG 5 cut(s) 18, 197, 376, 473, 1317
Sse9I AATT 5 cut(s) 98, 236, 655, 817, 1326
SseBI AGGCCT 1 cut(s) 871
SsiI CCGC 3 cut(s) 789, 917, 1073
SspMI CTAG 1 cut(s) 704
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 871
StyD4I CCNGG 3 cut(s) 325, 498, 943
TaaI ACNGT 3 cut(s) 690, 723, 1319
TaiI ACGT 3 cut(s) 355, 524, 1248
TaqI TCGA 6 cut(s) 376, 906, 1077, 1102, 1194, 1337
TaqII GACCGA 1 cut(s) 383
TasI AATT 5 cut(s) 98, 236, 655, 817, 1326
TfiI GAWTC 7 cut(s) 187, 558, 618, 848, 889, 908, 1104
Tru1I TTAA 5 cut(s) 57, 101, 273, 504, 642
Tru9I TTAA 5 cut(s) 57, 101, 273, 504, 642
TscAI CASTG 2 cut(s) 475, 1324
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 6 cut(s) 440, 674, 677, 775, 976, 1322
Tsp45I GTSAC 1 cut(s) 127
TspGWI ACGGA 2 cut(s) 440, 1289
TspRI CASTG 2 cut(s) 475, 1324
VpaK11BI GGWCC 2 cut(s) 395, 719
VspI ATTAAT 2 cut(s) 101, 273
XapI RAATTY 1 cut(s) 817
XceI RCATGY 1 cut(s) 196
XmnI GAANNNNTTC 2 cut(s) 206, 852
XspI CTAG 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.