Belongs to the glycosyl hydrolase 1 family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Glyco_hydro_1 | PF00232 | 65 - 549 | 8.2e-144 | Glycosyl hydrolase family 1 |
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 323 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 250 |
| Acc36I | ACCTGC | 1 cut(s) | 797 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 429 |
| AccII | CGCG | 2 cut(s) | 297, 887 |
| AciI | CCGC | 4 cut(s) | 38, 415, 885, 887 |
| AclWI | GGATC | 2 cut(s) | 297, 737 |
| AcoI | YGGCCR | 1 cut(s) | 1295 |
| AcsI | RAATTY | 8 cut(s) | 115, 148, 335, 406, 639, 867, 1145, 1434 |
| AcuI | CTGAAG | 1 cut(s) | 267 |
| AfaI | GTAC | 1 cut(s) | 283 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 429, 435 |
| AjnI | CCWGG | 2 cut(s) | 428, 739 |
| AluBI | AGCT | 8 cut(s) | 206, 456, 501, 572, 895, 1634, 1664, 1671 |
| AluI | AGCT | 8 cut(s) | 206, 456, 501, 572, 895, 1634, 1664, 1671 |
| Alw26I | GTCTC | 3 cut(s) | 786, 1469, 1521 |
| AlwI | GGATC | 2 cut(s) | 297, 737 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 704, 1315 |
| Ama87I | CYCGRG | 2 cut(s) | 78, 1659 |
| AoxI | GGCC | 1 cut(s) | 1295 |
| ApeKI | GCWGC | 5 cut(s) | 69, 110, 998, 1306, 1668 |
| ApoI | RAATTY | 8 cut(s) | 115, 148, 335, 406, 639, 867, 1145, 1434 |
| AseI | ATTAAT | 2 cut(s) | 471, 1158 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 620 |
| AspA2I | CCTAGG | 1 cut(s) | 1024 |
| AspLEI | GCGC | 2 cut(s) | 1218, 1602 |
| AspS9I | GGNCC | 4 cut(s) | 275, 809, 1279, 1423 |
| AsuC2I | CCSGG | 4 cut(s) | 79, 80, 142, 803 |
| AsuHPI | GGTGA | 3 cut(s) | 839, 941, 1096 |
| AsuII | TTCGAA | 2 cut(s) | 953, 1206 |
| AsuNHI | GCTAGC | 1 cut(s) | 1664 |
| AvaI | CYCGRG | 2 cut(s) | 78, 1659 |
| AvaII | GGWCC | 4 cut(s) | 275, 809, 1279, 1423 |
| AvrII | CCTAGG | 1 cut(s) | 1024 |
| BaeGI | GKGCMC | 1 cut(s) | 1293 |
| BbvI | GCAGC | 4 cut(s) | 56, 122, 1010, 1293 |
| BccI | CCATC | 6 cut(s) | 56, 95, 431, 1023, 1253, 1312 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1382, 1416 |
| BciT130I | CCWGG | 2 cut(s) | 430, 741 |
| BciVI | GTATCC | 1 cut(s) | 1496 |
| BclI | TGATCA | 5 cut(s) | 487, 516, 1128, 1411, 1648 |
| BcnI | CCSGG | 4 cut(s) | 79, 80, 142, 803 |
| BcoDI | GTCTC | 3 cut(s) | 786, 1469, 1521 |
| BfaI | CTAG | 6 cut(s) | 834, 1025, 1142, 1182, 1271, 1665 |
| BfmI | CTRYAG | 4 cut(s) | 342, 631, 705, 1307 |
| BfuAI | ACCTGC | 1 cut(s) | 797 |
| BfuI | GTATCC | 1 cut(s) | 1496 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 887, 1303 |
| BisI | GCNGC | 6 cut(s) | 70, 111, 888, 999, 1307, 1669 |
| BlnI | CCTAGG | 1 cut(s) | 1024 |
| BlpI | GCTNAGC | 1 cut(s) | 891 |
| BlsI | GCNGC | 6 cut(s) | 71, 112, 889, 1000, 1308, 1670 |
| Bme1390I | CCNGG | 6 cut(s) | 79, 80, 142, 430, 741, 803 |
| Bme18I | GGWCC | 4 cut(s) | 275, 809, 1279, 1423 |
| BmeT110I | CYCGRG | 2 cut(s) | 78, 1659 |
| BmgT120I | GGNCC | 4 cut(s) | 275, 809, 1279, 1423 |
| BmiI | GGNNCC | 2 cut(s) | 1041, 1425 |
| BmrFI | CCNGG | 6 cut(s) | 79, 80, 142, 430, 741, 803 |
| BmsI | GCATC | 2 cut(s) | 304, 1611 |
| BmtI | GCTAGC | 1 cut(s) | 1668 |
| BpmI | CTGGAG | 3 cut(s) | 451, 596, 768 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 891 |
| Bpu14I | TTCGAA | 2 cut(s) | 953, 1206 |
| BpuEI | CTTGAG | 2 cut(s) | 478, 740 |
| BpuMI | CCSGG | 4 cut(s) | 79, 80, 142, 803 |
| Bsa29I | ATCGAT | 1 cut(s) | 1578 |
| BsaAI | YACGTR | 1 cut(s) | 964 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1584 |
| BsaI | GGTCTC | 2 cut(s) | 786, 1521 |
| BsaJI | CCNNGG | 4 cut(s) | 78, 740, 885, 1024 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 404, 434, 569, 599, 651, 681, 756, 786 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 429, 435 |
| Bse1I | ACTGG | 2 cut(s) | 1298, 1517 |
| Bse3DI | GCAATG | 1 cut(s) | 60 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1584 |
| BseBI | CCWGG | 2 cut(s) | 430, 741 |
| BseCI | ATCGAT | 1 cut(s) | 1578 |
| BseDI | CCNNGG | 4 cut(s) | 78, 740, 885, 1024 |
| BseGI | GGATG | 6 cut(s) | 187, 1034, 1142, 1234, 1264, 1602 |
| BseJI | GATNNNNATC | 1 cut(s) | 1584 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 429, 435 |
| BseMI | GCAATG | 1 cut(s) | 60 |
| BseMII | CTCAG | 1 cut(s) | 1611 |
| BseNI | ACTGG | 2 cut(s) | 1298, 1517 |
| BseRI | GAGGAG | 1 cut(s) | 593 |
| BseSI | GKGCMC | 1 cut(s) | 1293 |
| BseXI | GCAGC | 4 cut(s) | 56, 122, 1010, 1293 |
| BseYI | CCCAGC | 1 cut(s) | 202 |
| BsgI | GTGCAG | 2 cut(s) | 55, 1095 |
| Bsh1236I | CGCG | 2 cut(s) | 297, 887 |
| BshFI | GGCC | 1 cut(s) | 1297 |
| BshVI | ATCGAT | 1 cut(s) | 1578 |
| BsiHKCI | CYCGRG | 2 cut(s) | 78, 1659 |
| BsiSI | CCGG | 5 cut(s) | 79, 126, 141, 803, 1298 |
| BslFI | GGGAC | 1 cut(s) | 785 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 429, 435 |
| BsmAI | GTCTC | 3 cut(s) | 786, 1469, 1521 |
| BsmBI | CGTCTC | 1 cut(s) | 1469 |
| BsmFI | GGGAC | 1 cut(s) | 785 |
| BsnI | GGCC | 1 cut(s) | 1297 |
| Bso31I | GGTCTC | 2 cut(s) | 786, 1521 |
| BsoBI | CYCGRG | 2 cut(s) | 78, 1659 |
| Bsp119I | TTCGAA | 2 cut(s) | 953, 1206 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1293 |
| Bsp143I | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 891 |
| BspACI | CCGC | 4 cut(s) | 38, 415, 885, 887 |
| BspANI | GGCC | 1 cut(s) | 1297 |
| BspCNI | CTCAG | 1 cut(s) | 1612 |
| BspDI | ATCGAT | 1 cut(s) | 1578 |
| BspFNI | CGCG | 2 cut(s) | 297, 887 |
| BspLI | GGNNCC | 2 cut(s) | 1041, 1425 |
| BspMAI | CTGCAG | 2 cut(s) | 709, 1311 |
| BspMI | ACCTGC | 1 cut(s) | 797 |
| BspOI | GCTAGC | 1 cut(s) | 1668 |
| BspPI | GGATC | 2 cut(s) | 297, 737 |
| BspT104I | TTCGAA | 2 cut(s) | 953, 1206 |
| BspTNI | GGTCTC | 2 cut(s) | 786, 1521 |
| BsrDI | GCAATG | 1 cut(s) | 60 |
| BsrI | ACTGG | 2 cut(s) | 1298, 1517 |
| BssECI | CCNNGG | 4 cut(s) | 78, 740, 885, 1024 |
| BssMI | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| BssT1I | CCWWGG | 1 cut(s) | 1024 |
| Bst2UI | CCWGG | 2 cut(s) | 430, 741 |
| Bst4CI | ACNGT | 5 cut(s) | 158, 635, 700, 794, 1283 |
| Bst6I | CTCTTC | 1 cut(s) | 1526 |
| BstBAI | YACGTR | 1 cut(s) | 964 |
| BstBI | TTCGAA | 2 cut(s) | 953, 1206 |
| BstC8I | GCNNGC | 2 cut(s) | 67, 1666 |
| BstDEI | CTNAG | 2 cut(s) | 891, 1620 |
| BstDSI | CCRYGG | 1 cut(s) | 885 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 433 |
| BstF5I | GGATG | 6 cut(s) | 187, 1034, 1142, 1234, 1264, 1602 |
| BstFNI | CGCG | 2 cut(s) | 297, 887 |
| BstHHI | GCGC | 2 cut(s) | 1218, 1602 |
| BstKTI | GATC | 9 cut(s) | 194, 292, 490, 519, 732, 1131, 1414, 1588, 1651 |
| BstMAI | GTCTC | 3 cut(s) | 786, 1469, 1521 |
| BstMBI | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 44, 53, 62, 233, 887, 1303 |
| BstNI | CCWGG | 2 cut(s) | 430, 741 |
| BstSCI | CCNGG | 6 cut(s) | 77, 78, 140, 428, 739, 801 |
| BstSFI | CTRYAG | 4 cut(s) | 342, 631, 705, 1307 |
| BstSLI | GKGCMC | 1 cut(s) | 1293 |
| BstUI | CGCG | 2 cut(s) | 297, 887 |
| BstV1I | GCAGC | 4 cut(s) | 56, 122, 1010, 1293 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 285 |
| Bsu15I | ATCGAT | 1 cut(s) | 1578 |
| BsuI | GTATCC | 1 cut(s) | 1496 |
| BsuRI | GGCC | 1 cut(s) | 1297 |
| BsuTUI | ATCGAT | 1 cut(s) | 1578 |
| BtgI | CCRYGG | 1 cut(s) | 885 |
| BtgZI | GCGATG | 2 cut(s) | 37, 278 |
| BtsCI | GGATG | 6 cut(s) | 187, 1034, 1142, 1234, 1264, 1602 |
| BtsIMutI | CAGTG | 2 cut(s) | 640, 1291 |
| BveI | ACCTGC | 1 cut(s) | 797 |
| Cac8I | GCNNGC | 2 cut(s) | 67, 1666 |
| CaiI | CAGNNNCTG | 2 cut(s) | 704, 1315 |
| CfoI | GCGC | 2 cut(s) | 1218, 1602 |
| Cfr13I | GGNCC | 4 cut(s) | 275, 809, 1279, 1423 |
| Cfr42I | CCGCGG | 1 cut(s) | 888 |
| Cfr9I | CCCGGG | 1 cut(s) | 78 |
| ClaI | ATCGAT | 1 cut(s) | 1578 |
| Csp6I | GTAC | 1 cut(s) | 282 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 805, 840 |
| CviAII | CATG | 5 cut(s) | 285, 813, 878, 1276, 1652 |
| CviQI | GTAC | 1 cut(s) | 282 |
| DdeI | CTNAG | 2 cut(s) | 891, 1620 |
| DpnI | GATC | 9 cut(s) | 193, 291, 489, 518, 731, 1130, 1413, 1587, 1650 |
| DpnII | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 250 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 250 |
| EaeI | YGGCCR | 1 cut(s) | 1295 |
| Eam1104I | CTCTTC | 1 cut(s) | 1526 |
| EarI | CTCTTC | 1 cut(s) | 1526 |
| Eco130I | CCWWGG | 1 cut(s) | 1024 |
| Eco31I | GGTCTC | 2 cut(s) | 786, 1521 |
| Eco47I | GGWCC | 4 cut(s) | 275, 809, 1279, 1423 |
| Eco57I | CTGAAG | 1 cut(s) | 267 |
| Eco88I | CYCGRG | 2 cut(s) | 78, 1659 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 433 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1423 |
| EcoRII | CCWGG | 2 cut(s) | 428, 739 |
| EcoT14I | CCWWGG | 1 cut(s) | 1024 |
| ErhI | CCWWGG | 1 cut(s) | 1024 |
| Esp3I | CGTCTC | 1 cut(s) | 1469 |
| FaeI | CATG | 5 cut(s) | 288, 816, 881, 1279, 1655 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 440, 472, 1623, 1655 |
| FaqI | GGGAC | 1 cut(s) | 785 |
| FatI | CATG | 5 cut(s) | 284, 812, 877, 1275, 1651 |
| FbaI | TGATCA | 5 cut(s) | 487, 516, 1128, 1411, 1648 |
| Fnu4HI | GCNGC | 6 cut(s) | 70, 111, 888, 999, 1307, 1669 |
| FokI | GGATG | 6 cut(s) | 194, 1041, 1149, 1241, 1271, 1589 |
| Fsp4HI | GCNGC | 6 cut(s) | 70, 111, 888, 999, 1307, 1669 |
| FspBI | CTAG | 6 cut(s) | 834, 1025, 1142, 1182, 1271, 1665 |
| GlaI | GCGC | 2 cut(s) | 1217, 1601 |
| GluI | GCNGC | 6 cut(s) | 70, 111, 888, 999, 1307, 1669 |
| GsaI | CCCAGC | 1 cut(s) | 206 |
| GsuI | CTGGAG | 3 cut(s) | 451, 596, 768 |
| HaeIII | GGCC | 1 cut(s) | 1297 |
| HapII | CCGG | 5 cut(s) | 79, 126, 141, 803, 1298 |
| HhaI | GCGC | 2 cut(s) | 1218, 1602 |
| Hin1II | CATG | 5 cut(s) | 288, 816, 881, 1279, 1655 |
| Hin6I | GCGC | 2 cut(s) | 1216, 1600 |
| HinP1I | GCGC | 2 cut(s) | 1216, 1600 |
| HindIII | AAGCTT | 2 cut(s) | 454, 1632 |
| HinfI | GANTC | 8 cut(s) | 95, 132, 222, 353, 830, 977, 1079, 1657 |
| HpaII | CCGG | 5 cut(s) | 79, 126, 141, 803, 1298 |
| HphI | GGTGA | 3 cut(s) | 839, 941, 1096 |
| Hpy166II | GTNNAC | 4 cut(s) | 681, 712, 752, 1492 |
| Hpy188I | TCNGA | 8 cut(s) | 100, 196, 401, 521, 559, 659, 976, 1366 |
| Hpy188III | TCNNGA | 8 cut(s) | 92, 170, 476, 541, 575, 613, 785, 1142 |
| Hpy8I | GTNNAC | 4 cut(s) | 681, 712, 752, 1492 |
| HpyAV | CCTTC | 9 cut(s) | 242, 257, 755, 1081, 1249, 1280, 1295, 1414, 1649 |
| HpyCH4III | ACNGT | 5 cut(s) | 158, 635, 700, 794, 1283 |
| HpyCH4IV | ACGT | 2 cut(s) | 963, 1494 |
| HpyCH4V | TGCA | 9 cut(s) | 72, 317, 707, 816, 998, 1112, 1309, 1403, 1555 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 44, 53, 62, 233, 887, 1303 |
| HpyF3I | CTNAG | 2 cut(s) | 891, 1620 |
| HpySE526I | ACGT | 2 cut(s) | 963, 1494 |
| Hsp92II | CATG | 5 cut(s) | 288, 816, 881, 1279, 1655 |
| HspAI | GCGC | 2 cut(s) | 1216, 1600 |
| Ksp22I | TGATCA | 5 cut(s) | 487, 516, 1128, 1411, 1648 |
| KspI | CCGCGG | 1 cut(s) | 888 |
| Kzo9I | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| LmnI | GCTCC | 1 cut(s) | 739 |
| Lsp1109I | GCAGC | 4 cut(s) | 56, 122, 1010, 1293 |
| LweI | GCATC | 2 cut(s) | 304, 1611 |
| MaeI | CTAG | 6 cut(s) | 834, 1025, 1142, 1182, 1271, 1665 |
| MaeII | ACGT | 2 cut(s) | 963, 1494 |
| MaeIII | GTNAC | 4 cut(s) | 184, 541, 593, 1654 |
| MalI | GATC | 9 cut(s) | 193, 291, 489, 518, 731, 1130, 1413, 1587, 1650 |
| MboI | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| MboII | GAAGA | 5 cut(s) | 185, 386, 998, 1253, 1513 |
| MfeI | CAATTG | 3 cut(s) | 348, 1211, 1415 |
| MhlI | GDGCHC | 1 cut(s) | 1293 |
| MlyI | GAGTC | 3 cut(s) | 231, 1088, 1651 |
| MnlI | CCTC | 7 cut(s) | 122, 241, 260, 266, 472, 571, 978 |
| MroXI | GAANNNNTTC | 1 cut(s) | 620 |
| MseI | TTAA | 5 cut(s) | 381, 471, 669, 1158, 1479 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1560 |
| MspA1I | CMGCKG | 2 cut(s) | 206, 887 |
| MspI | CCGG | 5 cut(s) | 79, 126, 141, 803, 1298 |
| MspR9I | CCNGG | 6 cut(s) | 79, 80, 142, 430, 741, 803 |
| MunI | CAATTG | 3 cut(s) | 348, 1211, 1415 |
| MvaI | CCWGG | 2 cut(s) | 430, 741 |
| MvnI | CGCG | 2 cut(s) | 297, 887 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 44, 53, 62, 233, 887, 1303 |
| NciI | CCSGG | 4 cut(s) | 79, 80, 142, 803 |
| NdeII | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| NheI | GCTAGC | 1 cut(s) | 1664 |
| NlaIII | CATG | 5 cut(s) | 288, 816, 881, 1279, 1655 |
| NlaIV | GGNNCC | 2 cut(s) | 1041, 1425 |
| NmuCI | GTSAC | 2 cut(s) | 541, 1654 |
| NspV | TTCGAA | 2 cut(s) | 953, 1206 |
| PaeR7I | CTCGAG | 1 cut(s) | 1659 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1491 |
| PdmI | GAANNNNTTC | 1 cut(s) | 620 |
| PfeI | GAWTC | 5 cut(s) | 95, 132, 353, 830, 977 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 429 |
| PfoI | TCCNGGA | 1 cut(s) | 428 |
| PkrI | GCNGC | 6 cut(s) | 71, 112, 889, 1000, 1308, 1670 |
| PleI | GAGTC | 3 cut(s) | 230, 1087, 1651 |
| PpsI | GAGTC | 3 cut(s) | 230, 1087, 1651 |
| Ppu21I | YACGTR | 1 cut(s) | 964 |
| PpuMI | RGGWCCY | 1 cut(s) | 1423 |
| PshBI | ATTAAT | 2 cut(s) | 471, 1158 |
| PsiI | TTATAA | 1 cut(s) | 323 |
| Psp5II | RGGWCCY | 1 cut(s) | 1423 |
| Psp6I | CCWGG | 2 cut(s) | 428, 739 |
| PspFI | CCCAGC | 1 cut(s) | 202 |
| PspGI | CCWGG | 2 cut(s) | 428, 739 |
| PspN4I | GGNNCC | 2 cut(s) | 1041, 1425 |
| PspPI | GGNCC | 4 cut(s) | 275, 809, 1279, 1423 |
| PspPPI | RGGWCCY | 1 cut(s) | 1423 |
| PspXI | VCTCGAGB | 1 cut(s) | 1659 |
| PstI | CTGCAG | 2 cut(s) | 709, 1311 |
| PstNI | CAGNNNCTG | 2 cut(s) | 704, 1315 |
| PvuII | CAGCTG | 1 cut(s) | 206 |
| RsaI | GTAC | 1 cut(s) | 283 |
| RsaNI | GTAC | 1 cut(s) | 282 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1560 |
| SacII | CCGCGG | 1 cut(s) | 888 |
| SaqAI | TTAA | 5 cut(s) | 381, 471, 669, 1158, 1479 |
| SatI | GCNGC | 6 cut(s) | 70, 111, 888, 999, 1307, 1669 |
| Sau3AI | GATC | 9 cut(s) | 191, 289, 487, 516, 729, 1128, 1411, 1585, 1648 |
| Sau96I | GGNCC | 4 cut(s) | 275, 809, 1279, 1423 |
| SchI | GAGTC | 3 cut(s) | 231, 1088, 1651 |
| ScrFI | CCNGG | 6 cut(s) | 79, 80, 142, 430, 741, 803 |
| SduI | GDGCHC | 1 cut(s) | 1293 |
| SfaNI | GCATC | 2 cut(s) | 304, 1611 |
| SfcI | CTRYAG | 4 cut(s) | 342, 631, 705, 1307 |
| Sfr274I | CTCGAG | 1 cut(s) | 1659 |
| Sfr303I | CCGCGG | 1 cut(s) | 888 |
| SfuI | TTCGAA | 2 cut(s) | 953, 1206 |
| SgrBI | CCGCGG | 1 cut(s) | 888 |
| SinI | GGWCC | 4 cut(s) | 275, 809, 1279, 1423 |
| SlaI | CTCGAG | 1 cut(s) | 1659 |
| SmaI | CCCGGG | 1 cut(s) | 80 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1560 |
| SmlI | CTYRAG | 3 cut(s) | 457, 755, 1659 |
| SmoI | CTYRAG | 3 cut(s) | 457, 755, 1659 |
| SsiI | CCGC | 4 cut(s) | 38, 415, 885, 887 |
| SspMI | CTAG | 6 cut(s) | 834, 1025, 1142, 1182, 1271, 1665 |
| StyD4I | CCNGG | 6 cut(s) | 77, 78, 140, 428, 739, 801 |
| StyI | CCWWGG | 1 cut(s) | 1024 |
| TaaI | ACNGT | 5 cut(s) | 158, 635, 700, 794, 1283 |
| TaiI | ACGT | 2 cut(s) | 966, 1497 |
| TaqI | TCGA | 7 cut(s) | 953, 1014, 1206, 1524, 1578, 1584, 1660 |
| TatI | WGTACW | 1 cut(s) | 281 |
| TauI | GCSGC | 1 cut(s) | 890 |
| TfiI | GAWTC | 5 cut(s) | 95, 132, 353, 830, 977 |
| Tru1I | TTAA | 5 cut(s) | 381, 471, 669, 1158, 1479 |
| Tru9I | TTAA | 5 cut(s) | 381, 471, 669, 1158, 1479 |
| TscAI | CASTG | 2 cut(s) | 640, 1298 |
| TseFI | GTSAC | 2 cut(s) | 541, 1654 |
| TseI | GCWGC | 5 cut(s) | 69, 110, 998, 1306, 1668 |
| Tsp45I | GTSAC | 2 cut(s) | 541, 1654 |
| TspDTI | ATGAA | 6 cut(s) | 345, 735, 1088, 1268, 1410, 1428 |
| TspMI | CCCGGG | 1 cut(s) | 78 |
| TspRI | CASTG | 2 cut(s) | 640, 1298 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 429 |
| VpaK11BI | GGWCC | 4 cut(s) | 275, 809, 1279, 1423 |
| VspI | ATTAAT | 2 cut(s) | 471, 1158 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 433 |
| XapI | RAATTY | 8 cut(s) | 115, 148, 335, 406, 639, 867, 1145, 1434 |
| XbaI | TCTAGA | 1 cut(s) | 1141 |
| XhoI | CTCGAG | 1 cut(s) | 1659 |
| XmaI | CCCGGG | 1 cut(s) | 78 |
| XmaJI | CCTAGG | 1 cut(s) | 1024 |
| XmnI | GAANNNNTTC | 1 cut(s) | 620 |
| XspI | CTAG | 6 cut(s) | 834, 1025, 1142, 1182, 1271, 1665 |