Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 694 |
| Acc36I | ACCTGC | 1 cut(s) | 694 |
| AccB1I | GGYRCC | 1 cut(s) | 695 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 390 |
| AccI | GTMKAC | 1 cut(s) | 285 |
| AciI | CCGC | 4 cut(s) | 277, 306, 527, 552 |
| AclWI | GGATC | 3 cut(s) | 220, 515, 668 |
| AcsI | RAATTY | 1 cut(s) | 266 |
| AfaI | GTAC | 2 cut(s) | 33, 680 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 390 |
| AflIII | ACRYGT | 1 cut(s) | 297 |
| AgsI | TTSAA | 2 cut(s) | 586, 717 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 242 |
| AjnI | CCWGG | 3 cut(s) | 271, 395, 698 |
| AluBI | AGCT | 2 cut(s) | 80, 533 |
| AluI | AGCT | 2 cut(s) | 80, 533 |
| AlwI | GGATC | 3 cut(s) | 220, 515, 668 |
| ApeKI | GCWGC | 2 cut(s) | 194, 197 |
| ApoI | RAATTY | 1 cut(s) | 266 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 581 |
| AspS9I | GGNCC | 4 cut(s) | 153, 236, 355, 393 |
| AsuHPI | GGTGA | 2 cut(s) | 97, 640 |
| AvaII | GGWCC | 4 cut(s) | 153, 236, 355, 393 |
| AxyI | CCTNAGG | 1 cut(s) | 408 |
| BanI | GGYRCC | 1 cut(s) | 695 |
| BbvCI | CCTCAGC | 1 cut(s) | 81 |
| BbvI | GCAGC | 2 cut(s) | 181, 184 |
| BccI | CCATC | 2 cut(s) | 317, 392 |
| BceAI | ACGGC | 1 cut(s) | 640 |
| BciT130I | CCWGG | 3 cut(s) | 273, 397, 700 |
| BfuAI | ACCTGC | 1 cut(s) | 694 |
| BisI | GCNGC | 3 cut(s) | 195, 198, 553 |
| BlsI | GCNGC | 3 cut(s) | 196, 199, 554 |
| Bme1390I | CCNGG | 3 cut(s) | 273, 397, 700 |
| Bme18I | GGWCC | 4 cut(s) | 153, 236, 355, 393 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 242 |
| BmgT120I | GGNCC | 4 cut(s) | 153, 236, 355, 393 |
| BmiI | GGNNCC | 2 cut(s) | 415, 697 |
| BmrFI | CCNGG | 3 cut(s) | 273, 397, 700 |
| BmsI | GCATC | 2 cut(s) | 131, 564 |
| Bpu10I | CCTNAGC | 1 cut(s) | 81 |
| BpuEI | CTTGAG | 3 cut(s) | 117, 335, 439 |
| BsaJI | CCNNGG | 4 cut(s) | 271, 272, 358, 656 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 612, 642 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 390 |
| Bse21I | CCTNAGG | 1 cut(s) | 408 |
| Bse3DI | GCAATG | 2 cut(s) | 48, 110 |
| BseBI | CCWGG | 3 cut(s) | 273, 397, 700 |
| BseDI | CCNNGG | 4 cut(s) | 271, 272, 358, 656 |
| BseGI | GGATG | 2 cut(s) | 45, 555 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 390 |
| BseMI | GCAATG | 2 cut(s) | 48, 110 |
| BseMII | CTCAG | 2 cut(s) | 72, 84 |
| BseXI | GCAGC | 2 cut(s) | 181, 184 |
| BsgI | GTGCAG | 1 cut(s) | 183 |
| BshNI | GGYRCC | 1 cut(s) | 695 |
| BslFI | GGGAC | 1 cut(s) | 263 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 390 |
| BsmFI | GGGAC | 1 cut(s) | 263 |
| BsmI | GAATGC | 1 cut(s) | 94 |
| Bsp1407I | TGTACA | 1 cut(s) | 31 |
| Bsp143I | GATC | 3 cut(s) | 212, 520, 660 |
| BspACI | CCGC | 4 cut(s) | 277, 306, 527, 552 |
| BspCNI | CTCAG | 2 cut(s) | 73, 85 |
| BspLI | GGNNCC | 2 cut(s) | 415, 697 |
| BspMI | ACCTGC | 1 cut(s) | 694 |
| BspPI | GGATC | 3 cut(s) | 220, 515, 668 |
| BspT107I | GGYRCC | 1 cut(s) | 695 |
| BsrDI | GCAATG | 2 cut(s) | 48, 110 |
| BsrGI | TGTACA | 1 cut(s) | 31 |
| BssECI | CCNNGG | 4 cut(s) | 271, 272, 358, 656 |
| BssMI | GATC | 3 cut(s) | 212, 520, 660 |
| BssT1I | CCWWGG | 1 cut(s) | 358 |
| Bst2UI | CCWGG | 3 cut(s) | 273, 397, 700 |
| Bst4CI | ACNGT | 2 cut(s) | 596, 722 |
| Bst6I | CTCTTC | 2 cut(s) | 143, 226 |
| BstAUI | TGTACA | 1 cut(s) | 31 |
| BstC8I | GCNNGC | 1 cut(s) | 531 |
| BstDEI | CTNAG | 4 cut(s) | 17, 81, 93, 408 |
| BstDSI | CCRYGG | 1 cut(s) | 656 |
| BstF5I | GGATG | 2 cut(s) | 45, 555 |
| BstKTI | GATC | 3 cut(s) | 215, 523, 663 |
| BstMBI | GATC | 3 cut(s) | 212, 520, 660 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 224 |
| BstNI | CCWGG | 3 cut(s) | 273, 397, 700 |
| BstNSI | RCATGY | 1 cut(s) | 301 |
| BstSCI | CCNGG | 3 cut(s) | 271, 395, 698 |
| BstV1I | GCAGC | 2 cut(s) | 181, 184 |
| BstX2I | RGATCY | 1 cut(s) | 212 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 246 |
| BstYI | RGATCY | 1 cut(s) | 212 |
| Bsu36I | CCTNAGG | 1 cut(s) | 408 |
| BtgI | CCRYGG | 1 cut(s) | 656 |
| BtsCI | GGATG | 2 cut(s) | 45, 555 |
| BtsIMutI | CAGTG | 1 cut(s) | 601 |
| BveI | ACCTGC | 1 cut(s) | 694 |
| Cac8I | GCNNGC | 1 cut(s) | 531 |
| Cfr13I | GGNCC | 4 cut(s) | 153, 236, 355, 393 |
| Csp6I | GTAC | 2 cut(s) | 32, 679 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 722 |
| CviAII | CATG | 3 cut(s) | 298, 664, 730 |
| CviJI | RGCY | 4 cut(s) | 80, 109, 533, 655 |
| CviKI_1 | RGCY | 4 cut(s) | 80, 109, 533, 655 |
| CviQI | GTAC | 2 cut(s) | 32, 679 |
| DdeI | CTNAG | 4 cut(s) | 17, 81, 93, 408 |
| DpnI | GATC | 3 cut(s) | 214, 522, 662 |
| DpnII | GATC | 3 cut(s) | 212, 520, 660 |
| DraI | TTTAAA | 1 cut(s) | 610 |
| DriI | GACNNNNNGTC | 1 cut(s) | 242 |
| Eam1104I | CTCTTC | 2 cut(s) | 143, 226 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 242 |
| EarI | CTCTTC | 2 cut(s) | 143, 226 |
| EciI | GGCGGA | 1 cut(s) | 292 |
| Eco130I | CCWWGG | 1 cut(s) | 358 |
| Eco47I | GGWCC | 4 cut(s) | 153, 236, 355, 393 |
| Eco81I | CCTNAGG | 1 cut(s) | 408 |
| EcoO109I | RGGNCCY | 1 cut(s) | 355 |
| EcoRII | CCWGG | 3 cut(s) | 271, 395, 698 |
| EcoT14I | CCWWGG | 1 cut(s) | 358 |
| EcoT22I | ATGCAT | 1 cut(s) | 124 |
| ErhI | CCWWGG | 1 cut(s) | 358 |
| FaeI | CATG | 3 cut(s) | 301, 667, 733 |
| FaiI | YATR | 6 cut(s) | 299, 319, 509, 549, 665, 731 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 401, 433 |
| FaqI | GGGAC | 1 cut(s) | 263 |
| FatI | CATG | 3 cut(s) | 297, 663, 729 |
| FblI | GTMKAC | 1 cut(s) | 285 |
| Fnu4HI | GCNGC | 3 cut(s) | 195, 198, 553 |
| FokI | GGATG | 2 cut(s) | 52, 542 |
| Fsp4HI | GCNGC | 3 cut(s) | 195, 198, 553 |
| GluI | GCNGC | 3 cut(s) | 195, 198, 553 |
| Hin1II | CATG | 3 cut(s) | 301, 667, 733 |
| HindIII | AAGCTT | 1 cut(s) | 531 |
| HinfI | GANTC | 4 cut(s) | 314, 346, 368, 400 |
| HphI | GGTGA | 2 cut(s) | 97, 640 |
| Hpy166II | GTNNAC | 5 cut(s) | 286, 303, 433, 599, 642 |
| Hpy188I | TCNGA | 2 cut(s) | 157, 182 |
| Hpy188III | TCNNGA | 3 cut(s) | 134, 481, 574 |
| Hpy8I | GTNNAC | 5 cut(s) | 286, 303, 433, 599, 642 |
| HpyAV | CCTTC | 2 cut(s) | 203, 580 |
| HpyCH4III | ACNGT | 2 cut(s) | 596, 722 |
| HpyCH4V | TGCA | 6 cut(s) | 115, 122, 200, 218, 514, 733 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 224 |
| HpyF3I | CTNAG | 4 cut(s) | 17, 81, 93, 408 |
| Hsp92II | CATG | 3 cut(s) | 301, 667, 733 |
| Kzo9I | GATC | 3 cut(s) | 212, 520, 660 |
| LmnI | GCTCC | 1 cut(s) | 572 |
| LpnPI | CCDG | 9 cut(s) | 213, 232, 258, 285, 382, 409, 685, 689, 712 |
| Lsp1109I | GCAGC | 2 cut(s) | 181, 184 |
| LweI | GCATC | 2 cut(s) | 131, 564 |
| MaeIII | GTNAC | 3 cut(s) | 72, 184, 502 |
| MalI | GATC | 3 cut(s) | 214, 522, 662 |
| MboI | GATC | 3 cut(s) | 212, 520, 660 |
| MboII | GAAGA | 5 cut(s) | 160, 243, 347, 355, 395 |
| MflI | RGATCY | 1 cut(s) | 212 |
| MluCI | AATT | 2 cut(s) | 266, 634 |
| MlyI | GAGTC | 1 cut(s) | 308 |
| MmeI | TCCRAC | 1 cut(s) | 180 |
| MnlI | CCTC | 9 cut(s) | 18, 24, 76, 76, 141, 144, 227, 433, 569 |
| Mph1103I | ATGCAT | 1 cut(s) | 124 |
| MroXI | GAANNNNTTC | 1 cut(s) | 581 |
| MseI | TTAA | 1 cut(s) | 609 |
| MslI | CAYNNNNRTG | 1 cut(s) | 296 |
| MspR9I | CCNGG | 3 cut(s) | 273, 397, 700 |
| Mva1269I | GAATGC | 1 cut(s) | 94 |
| MvaI | CCWGG | 3 cut(s) | 273, 397, 700 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 224 |
| NdeII | GATC | 3 cut(s) | 212, 520, 660 |
| NlaIII | CATG | 3 cut(s) | 301, 667, 733 |
| NlaIV | GGNNCC | 2 cut(s) | 415, 697 |
| NmuCI | GTSAC | 3 cut(s) | 72, 184, 502 |
| NsiI | ATGCAT | 1 cut(s) | 124 |
| NspI | RCATGY | 1 cut(s) | 301 |
| PaqCI | CACCTGC | 1 cut(s) | 694 |
| PasI | CCCWGGG | 1 cut(s) | 272 |
| PciI | ACATGT | 1 cut(s) | 297 |
| PctI | GAATGC | 1 cut(s) | 94 |
| PdmI | GAANNNNTTC | 1 cut(s) | 581 |
| PfeI | GAWTC | 3 cut(s) | 346, 368, 400 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 390 |
| PkrI | GCNGC | 3 cut(s) | 196, 199, 554 |
| PleI | GAGTC | 1 cut(s) | 308 |
| PpsI | GAGTC | 1 cut(s) | 308 |
| PpuMI | RGGWCCY | 1 cut(s) | 355 |
| PscI | ACATGT | 1 cut(s) | 297 |
| Psp5II | RGGWCCY | 1 cut(s) | 355 |
| Psp6I | CCWGG | 3 cut(s) | 271, 395, 698 |
| PspGI | CCWGG | 3 cut(s) | 271, 395, 698 |
| PspN4I | GGNNCC | 2 cut(s) | 415, 697 |
| PspPI | GGNCC | 4 cut(s) | 153, 236, 355, 393 |
| PspPPI | RGGWCCY | 1 cut(s) | 355 |
| PsuI | RGATCY | 1 cut(s) | 212 |
| RsaI | GTAC | 2 cut(s) | 33, 680 |
| RsaNI | GTAC | 2 cut(s) | 32, 679 |
| RseI | CAYNNNNRTG | 1 cut(s) | 296 |
| SaqAI | TTAA | 1 cut(s) | 609 |
| SatI | GCNGC | 3 cut(s) | 195, 198, 553 |
| Sau3AI | GATC | 3 cut(s) | 212, 520, 660 |
| Sau96I | GGNCC | 4 cut(s) | 153, 236, 355, 393 |
| SchI | GAGTC | 1 cut(s) | 308 |
| ScrFI | CCNGG | 3 cut(s) | 273, 397, 700 |
| SfaNI | GCATC | 2 cut(s) | 131, 564 |
| SinI | GGWCC | 4 cut(s) | 153, 236, 355, 393 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 296 |
| SmlI | CTYRAG | 3 cut(s) | 132, 350, 418 |
| SmoI | CTYRAG | 3 cut(s) | 132, 350, 418 |
| Sse9I | AATT | 2 cut(s) | 266, 634 |
| SsiI | CCGC | 4 cut(s) | 277, 306, 527, 552 |
| StyD4I | CCNGG | 3 cut(s) | 271, 395, 698 |
| StyI | CCWWGG | 1 cut(s) | 358 |
| TaaI | ACNGT | 2 cut(s) | 596, 722 |
| TaqI | TCGA | 1 cut(s) | 312 |
| TasI | AATT | 2 cut(s) | 266, 634 |
| TatI | WGTACW | 1 cut(s) | 31 |
| TauI | GCSGC | 1 cut(s) | 555 |
| TfiI | GAWTC | 3 cut(s) | 346, 368, 400 |
| Tru1I | TTAA | 1 cut(s) | 609 |
| Tru9I | TTAA | 1 cut(s) | 609 |
| TscAI | CASTG | 1 cut(s) | 601 |
| TseFI | GTSAC | 3 cut(s) | 72, 184, 502 |
| TseI | GCWGC | 2 cut(s) | 194, 197 |
| Tsp45I | GTSAC | 3 cut(s) | 72, 184, 502 |
| TspDTI | ATGAA | 1 cut(s) | 454 |
| TspRI | CASTG | 1 cut(s) | 601 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 390 |
| VpaK11BI | GGWCC | 4 cut(s) | 153, 236, 355, 393 |
| XapI | RAATTY | 1 cut(s) | 266 |
| XceI | RCATGY | 1 cut(s) | 301 |
| XmiI | GTMKAC | 1 cut(s) | 285 |
| XmnI | GAANNNNTTC | 1 cut(s) | 581 |
| Zsp2I | ATGCAT | 1 cut(s) | 124 |