Belongs to the glycosyl hydrolase 1 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 746 |
| AarI | CACCTGC | 1 cut(s) | 780 |
| Acc36I | ACCTGC | 1 cut(s) | 780 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 474 |
| AccI | GTMKAC | 1 cut(s) | 1605 |
| AccII | CGCG | 1 cut(s) | 1181 |
| AciI | CCGC | 2 cut(s) | 773, 1179 |
| AclI | AACGTT | 1 cut(s) | 1197 |
| AclWI | GGATC | 8 cut(s) | 217, 256, 599, 1140, 1153, 1445, 1458, 1499 |
| AcoI | YGGCCR | 1 cut(s) | 750 |
| AcsI | RAATTY | 5 cut(s) | 155, 1017, 1349, 1401, 1660 |
| AcuI | CTGAAG | 3 cut(s) | 150, 1140, 1319 |
| AfaI | GTAC | 3 cut(s) | 801, 807, 1487 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 474, 480 |
| AflIII | ACRYGT | 1 cut(s) | 381 |
| AgsI | TTSAA | 5 cut(s) | 76, 139, 206, 670, 1466 |
| AjnI | CCWGG | 3 cut(s) | 265, 473, 784 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 327, 359 |
| AluBI | AGCT | 3 cut(s) | 904, 1004, 1214 |
| AluI | AGCT | 3 cut(s) | 904, 1004, 1214 |
| Alw21I | GWGCWC | 1 cut(s) | 11 |
| Alw26I | GTCTC | 1 cut(s) | 92 |
| AlwI | GGATC | 8 cut(s) | 217, 256, 599, 1140, 1153, 1445, 1458, 1499 |
| AoxI | GGCC | 1 cut(s) | 750 |
| ApeKI | GCWGC | 4 cut(s) | 191, 194, 776, 1004 |
| ApoI | RAATTY | 5 cut(s) | 155, 1017, 1349, 1401, 1660 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 225, 257 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 665, 1470 |
| AspLEI | GCGC | 1 cut(s) | 1629 |
| AspS9I | GGNCC | 2 cut(s) | 233, 1286 |
| AsuHPI | GGTGA | 2 cut(s) | 3, 1528 |
| AsuII | TTCGAA | 1 cut(s) | 1029 |
| AvaII | GGWCC | 2 cut(s) | 233, 1286 |
| BalI | TGGCCA | 1 cut(s) | 752 |
| BamHI | GGATCC | 2 cut(s) | 1145, 1450 |
| BanII | GRGCYC | 1 cut(s) | 1546 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1349, 1381 |
| BbsI | GAAGAC | 2 cut(s) | 1002, 1325 |
| Bbv12I | GWGCWC | 1 cut(s) | 11 |
| BbvI | GCAGC | 4 cut(s) | 178, 181, 763, 991 |
| BccI | CCATC | 7 cut(s) | 313, 476, 629, 942, 1032, 1292, 1492 |
| BciT130I | CCWGG | 3 cut(s) | 267, 475, 786 |
| BclI | TGATCA | 2 cut(s) | 532, 1137 |
| BcoDI | GTCTC | 1 cut(s) | 92 |
| BfaI | CTAG | 1 cut(s) | 480 |
| BfmI | CTRYAG | 1 cut(s) | 907 |
| BfuAI | ACCTGC | 1 cut(s) | 780 |
| BisI | GCNGC | 5 cut(s) | 192, 195, 774, 777, 1005 |
| BlsI | GCNGC | 5 cut(s) | 193, 196, 775, 778, 1006 |
| Bme1390I | CCNGG | 3 cut(s) | 267, 475, 786 |
| Bme18I | GGWCC | 2 cut(s) | 233, 1286 |
| BmgT120I | GGNCC | 2 cut(s) | 233, 1286 |
| BmiI | GGNNCC | 3 cut(s) | 1050, 1147, 1452 |
| BmrFI | CCNGG | 3 cut(s) | 267, 475, 786 |
| BmrI | ACTGGG | 1 cut(s) | 1549 |
| BmsI | GCATC | 5 cut(s) | 128, 582, 815, 1173, 1314 |
| BmuI | ACTGGG | 1 cut(s) | 1549 |
| BpiI | GAAGAC | 2 cut(s) | 1002, 1325 |
| Bpu14I | TTCGAA | 1 cut(s) | 1029 |
| BpuEI | CTTGAG | 1 cut(s) | 1607 |
| BsaBI | GATNNNNATC | 1 cut(s) | 728 |
| BsaI | GGTCTC | 1 cut(s) | 92 |
| BsaJI | CCNNGG | 2 cut(s) | 265, 442 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 696, 726 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 474, 480 |
| Bse1I | ACTGG | 1 cut(s) | 1544 |
| Bse3DI | GCAATG | 1 cut(s) | 1509 |
| Bse8I | GATNNNNATC | 1 cut(s) | 728 |
| BseBI | CCWGG | 3 cut(s) | 267, 475, 786 |
| BseDI | CCNNGG | 2 cut(s) | 265, 442 |
| BseGI | GGATG | 4 cut(s) | 45, 830, 953, 1043 |
| BseJI | GATNNNNATC | 1 cut(s) | 728 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 474, 480 |
| BseMI | GCAATG | 1 cut(s) | 1509 |
| BseMII | CTCAG | 1 cut(s) | 87 |
| BseNI | ACTGG | 1 cut(s) | 1544 |
| BseRI | GAGGAG | 1 cut(s) | 525 |
| BseXI | GCAGC | 4 cut(s) | 178, 181, 763, 991 |
| BsgI | GTGCAG | 1 cut(s) | 180 |
| Bsh1236I | CGCG | 1 cut(s) | 1181 |
| BshFI | GGCC | 1 cut(s) | 752 |
| BsiHKAI | GWGCWC | 1 cut(s) | 11 |
| BslFI | GGGAC | 1 cut(s) | 260 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 474, 480 |
| BsmAI | GTCTC | 1 cut(s) | 92 |
| BsmFI | GGGAC | 1 cut(s) | 260 |
| BsmI | GAATGC | 5 cut(s) | 97, 121, 1470, 1472, 1629 |
| BsnI | GGCC | 1 cut(s) | 752 |
| Bso31I | GGTCTC | 1 cut(s) | 92 |
| Bsp119I | TTCGAA | 1 cut(s) | 1029 |
| Bsp1286I | GDGCHC | 2 cut(s) | 11, 1546 |
| Bsp1407I | TGTACA | 1 cut(s) | 1485 |
| Bsp143I | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| BspACI | CCGC | 2 cut(s) | 773, 1179 |
| BspANI | GGCC | 1 cut(s) | 752 |
| BspCNI | CTCAG | 1 cut(s) | 88 |
| BspFNI | CGCG | 1 cut(s) | 1181 |
| BspHI | TCATGA | 1 cut(s) | 1353 |
| BspLI | GGNNCC | 3 cut(s) | 1050, 1147, 1452 |
| BspMI | ACCTGC | 1 cut(s) | 780 |
| BspPI | GGATC | 8 cut(s) | 217, 256, 599, 1140, 1153, 1445, 1458, 1499 |
| BspT104I | TTCGAA | 1 cut(s) | 1029 |
| BspTNI | GGTCTC | 1 cut(s) | 92 |
| BsrDI | GCAATG | 1 cut(s) | 1509 |
| BsrGI | TGTACA | 1 cut(s) | 1485 |
| BsrI | ACTGG | 1 cut(s) | 1544 |
| BssECI | CCNNGG | 2 cut(s) | 265, 442 |
| BssMI | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| BssT1I | CCWWGG | 1 cut(s) | 442 |
| Bst2UI | CCWGG | 3 cut(s) | 267, 475, 786 |
| Bst4CI | ACNGT | 3 cut(s) | 545, 680, 895 |
| Bst6I | CTCTTC | 1 cut(s) | 1119 |
| BstAUI | TGTACA | 1 cut(s) | 1485 |
| BstBI | TTCGAA | 1 cut(s) | 1029 |
| BstDEI | CTNAG | 2 cut(s) | 96, 1647 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 478 |
| BstF5I | GGATG | 4 cut(s) | 45, 830, 953, 1043 |
| BstFNI | CGCG | 1 cut(s) | 1181 |
| BstHHI | GCGC | 1 cut(s) | 1629 |
| BstKTI | GATC | 9 cut(s) | 212, 264, 535, 607, 769, 1140, 1148, 1453, 1494 |
| BstMAI | GTCTC | 1 cut(s) | 92 |
| BstMBI | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 92, 221, 1211 |
| BstNI | CCWGG | 3 cut(s) | 267, 475, 786 |
| BstNSI | RCATGY | 1 cut(s) | 385 |
| BstSCI | CCNGG | 3 cut(s) | 265, 473, 784 |
| BstSFI | CTRYAG | 1 cut(s) | 907 |
| BstUI | CGCG | 1 cut(s) | 1181 |
| BstV1I | GCAGC | 4 cut(s) | 178, 181, 763, 991 |
| BstV2I | GAAGAC | 2 cut(s) | 1002, 1325 |
| BstX2I | RGATCY | 3 cut(s) | 261, 1145, 1450 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 243 |
| BstYI | RGATCY | 3 cut(s) | 261, 1145, 1450 |
| BsuRI | GGCC | 1 cut(s) | 752 |
| BtsCI | GGATG | 4 cut(s) | 45, 830, 953, 1043 |
| BtsI | GCAGTG | 1 cut(s) | 186 |
| BtsIMutI | CAGTG | 4 cut(s) | 186, 685, 1116, 1537 |
| BveI | ACCTGC | 1 cut(s) | 780 |
| CciI | TCATGA | 1 cut(s) | 1353 |
| CfoI | GCGC | 1 cut(s) | 1629 |
| Cfr13I | GGNCC | 2 cut(s) | 233, 1286 |
| Csp6I | GTAC | 3 cut(s) | 800, 806, 1486 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 600, 635 |
| CviAII | CATG | 7 cut(s) | 18, 112, 382, 887, 1045, 1292, 1354 |
| CviQI | GTAC | 3 cut(s) | 800, 806, 1486 |
| DdeI | CTNAG | 2 cut(s) | 96, 1647 |
| DpnI | GATC | 9 cut(s) | 211, 263, 534, 606, 768, 1139, 1147, 1452, 1493 |
| DpnII | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| DraI | TTTAAA | 1 cut(s) | 645 |
| EaeI | YGGCCR | 1 cut(s) | 750 |
| Eam1104I | CTCTTC | 1 cut(s) | 1119 |
| EarI | CTCTTC | 1 cut(s) | 1119 |
| Eco130I | CCWWGG | 1 cut(s) | 442 |
| Eco24I | GRGCYC | 1 cut(s) | 1546 |
| Eco31I | GGTCTC | 1 cut(s) | 92 |
| Eco47I | GGWCC | 2 cut(s) | 233, 1286 |
| Eco57I | CTGAAG | 3 cut(s) | 150, 1140, 1319 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 478 |
| EcoRI | GAATTC | 2 cut(s) | 155, 1349 |
| EcoRII | CCWGG | 3 cut(s) | 265, 473, 784 |
| EcoT14I | CCWWGG | 1 cut(s) | 442 |
| EcoT22I | ATGCAT | 2 cut(s) | 121, 1472 |
| EcoT38I | GRGCYC | 1 cut(s) | 1546 |
| ErhI | CCWWGG | 1 cut(s) | 442 |
| FaeI | CATG | 7 cut(s) | 21, 115, 385, 890, 1048, 1295, 1357 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1133, 1165, 1563, 1595 |
| FaqI | GGGAC | 1 cut(s) | 260 |
| FatI | CATG | 7 cut(s) | 17, 111, 381, 886, 1044, 1291, 1353 |
| FbaI | TGATCA | 2 cut(s) | 532, 1137 |
| FblI | GTMKAC | 1 cut(s) | 1605 |
| Fnu4HI | GCNGC | 5 cut(s) | 192, 195, 774, 777, 1005 |
| FokI | GGATG | 4 cut(s) | 52, 837, 960, 1050 |
| FriOI | GRGCYC | 1 cut(s) | 1546 |
| Fsp4HI | GCNGC | 5 cut(s) | 192, 195, 774, 777, 1005 |
| FspBI | CTAG | 1 cut(s) | 480 |
| GlaI | GCGC | 1 cut(s) | 1628 |
| GluI | GCNGC | 5 cut(s) | 192, 195, 774, 777, 1005 |
| HaeIII | GGCC | 1 cut(s) | 752 |
| HhaI | GCGC | 1 cut(s) | 1629 |
| Hin1II | CATG | 7 cut(s) | 21, 115, 385, 890, 1048, 1295, 1357 |
| Hin6I | GCGC | 1 cut(s) | 1627 |
| HinP1I | GCGC | 1 cut(s) | 1627 |
| HincII | GTYRAC | 2 cut(s) | 973, 1606 |
| HindII | GTYRAC | 2 cut(s) | 973, 1606 |
| HphI | GGTGA | 2 cut(s) | 3, 1528 |
| Hpy166II | GTNNAC | 7 cut(s) | 150, 387, 517, 683, 938, 973, 1606 |
| Hpy188I | TCNGA | 6 cut(s) | 358, 985, 1338, 1393, 1408, 1592 |
| Hpy188III | TCNNGA | 3 cut(s) | 565, 1354, 1495 |
| Hpy8I | GTNNAC | 7 cut(s) | 150, 387, 517, 683, 938, 973, 1606 |
| HpyAV | CCTTC | 3 cut(s) | 200, 1159, 1565 |
| HpyCH4III | ACNGT | 3 cut(s) | 545, 680, 895 |
| HpyCH4IV | ACGT | 3 cut(s) | 141, 1197, 1239 |
| HpyCH4V | TGCA | 7 cut(s) | 119, 197, 828, 1007, 1268, 1470, 1583 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 92, 221, 1211 |
| HpyF3I | CTNAG | 2 cut(s) | 96, 1647 |
| HpySE526I | ACGT | 3 cut(s) | 141, 1197, 1239 |
| Hsp92II | CATG | 7 cut(s) | 21, 115, 385, 890, 1048, 1295, 1357 |
| HspAI | GCGC | 1 cut(s) | 1627 |
| Ksp22I | TGATCA | 2 cut(s) | 532, 1137 |
| Kzo9I | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| LmnI | GCTCC | 2 cut(s) | 656, 784 |
| Lsp1109I | GCAGC | 4 cut(s) | 178, 181, 763, 991 |
| LweI | GCATC | 5 cut(s) | 128, 582, 815, 1173, 1314 |
| MaeI | CTAG | 1 cut(s) | 480 |
| MaeII | ACGT | 3 cut(s) | 141, 1197, 1239 |
| MaeIII | GTNAC | 3 cut(s) | 586, 862, 951 |
| MalI | GATC | 9 cut(s) | 211, 263, 534, 606, 768, 1139, 1147, 1452, 1493 |
| MboI | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| MflI | RGATCY | 3 cut(s) | 261, 1145, 1450 |
| MhlI | GDGCHC | 2 cut(s) | 11, 1546 |
| MlsI | TGGCCA | 1 cut(s) | 752 |
| MluNI | TGGCCA | 1 cut(s) | 752 |
| MlyI | GAGTC | 4 cut(s) | 189, 1284, 1381, 1425 |
| MnlI | CCTC | 5 cut(s) | 138, 503, 517, 890, 1299 |
| Mox20I | TGGCCA | 1 cut(s) | 752 |
| Mph1103I | ATGCAT | 2 cut(s) | 121, 1472 |
| MroXI | GAANNNNTTC | 2 cut(s) | 665, 1470 |
| MscI | TGGCCA | 1 cut(s) | 752 |
| MseI | TTAA | 6 cut(s) | 548, 644, 693, 900, 1280, 1667 |
| MslI | CAYNNNNRTG | 3 cut(s) | 16, 114, 1296 |
| Msp20I | TGGCCA | 1 cut(s) | 752 |
| MspR9I | CCNGG | 3 cut(s) | 267, 475, 786 |
| Mva1269I | GAATGC | 5 cut(s) | 97, 121, 1470, 1472, 1629 |
| MvaI | CCWGG | 3 cut(s) | 267, 475, 786 |
| MvnI | CGCG | 1 cut(s) | 1181 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 92, 221, 1211 |
| NdeII | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| NlaIII | CATG | 7 cut(s) | 21, 115, 385, 890, 1048, 1295, 1357 |
| NlaIV | GGNNCC | 3 cut(s) | 1050, 1147, 1452 |
| NmuCI | GTSAC | 2 cut(s) | 862, 951 |
| NsiI | ATGCAT | 2 cut(s) | 121, 1472 |
| NspI | RCATGY | 1 cut(s) | 385 |
| NspV | TTCGAA | 1 cut(s) | 1029 |
| PagI | TCATGA | 1 cut(s) | 1353 |
| PaqCI | CACCTGC | 1 cut(s) | 780 |
| PciI | ACATGT | 1 cut(s) | 381 |
| PctI | GAATGC | 5 cut(s) | 97, 121, 1470, 1472, 1629 |
| PdmI | GAANNNNTTC | 2 cut(s) | 665, 1470 |
| PfeI | GAWTC | 6 cut(s) | 304, 353, 398, 452, 484, 1640 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 474 |
| PfoI | TCCNGGA | 1 cut(s) | 473 |
| PkrI | GCNGC | 5 cut(s) | 193, 196, 775, 778, 1006 |
| PleI | GAGTC | 4 cut(s) | 188, 1283, 1380, 1425 |
| PpsI | GAGTC | 4 cut(s) | 188, 1283, 1380, 1425 |
| PscI | ACATGT | 1 cut(s) | 381 |
| PsiI | TTATAA | 1 cut(s) | 746 |
| Psp1406I | AACGTT | 1 cut(s) | 1197 |
| Psp6I | CCWGG | 3 cut(s) | 265, 473, 784 |
| PspGI | CCWGG | 3 cut(s) | 265, 473, 784 |
| PspN4I | GGNNCC | 3 cut(s) | 1050, 1147, 1452 |
| PspPI | GGNCC | 2 cut(s) | 233, 1286 |
| PsuI | RGATCY | 3 cut(s) | 261, 1145, 1450 |
| RsaI | GTAC | 3 cut(s) | 801, 807, 1487 |
| RsaNI | GTAC | 3 cut(s) | 800, 806, 1486 |
| RseI | CAYNNNNRTG | 3 cut(s) | 16, 114, 1296 |
| SalI | GTCGAC | 1 cut(s) | 1604 |
| SaqAI | TTAA | 6 cut(s) | 548, 644, 693, 900, 1280, 1667 |
| SatI | GCNGC | 5 cut(s) | 192, 195, 774, 777, 1005 |
| Sau3AI | GATC | 9 cut(s) | 209, 261, 532, 604, 766, 1137, 1145, 1450, 1491 |
| Sau96I | GGNCC | 2 cut(s) | 233, 1286 |
| SchI | GAGTC | 4 cut(s) | 189, 1284, 1381, 1425 |
| ScrFI | CCNGG | 3 cut(s) | 267, 475, 786 |
| SduI | GDGCHC | 2 cut(s) | 11, 1546 |
| SfaNI | GCATC | 5 cut(s) | 128, 582, 815, 1173, 1314 |
| SfcI | CTRYAG | 1 cut(s) | 907 |
| SfuI | TTCGAA | 1 cut(s) | 1029 |
| SinI | GGWCC | 2 cut(s) | 233, 1286 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 16, 114, 1296 |
| SmlI | CTYRAG | 1 cut(s) | 1622 |
| SmoI | CTYRAG | 1 cut(s) | 1622 |
| SsiI | CCGC | 2 cut(s) | 773, 1179 |
| SspMI | CTAG | 1 cut(s) | 480 |
| StyD4I | CCNGG | 3 cut(s) | 265, 473, 784 |
| StyI | CCWWGG | 1 cut(s) | 442 |
| TaaI | ACNGT | 3 cut(s) | 545, 680, 895 |
| TaiI | ACGT | 3 cut(s) | 144, 1200, 1242 |
| TaqI | TCGA | 7 cut(s) | 1029, 1152, 1210, 1434, 1477, 1551, 1605 |
| TatI | WGTACW | 2 cut(s) | 799, 1485 |
| TauI | GCSGC | 1 cut(s) | 776 |
| TfiI | GAWTC | 6 cut(s) | 304, 353, 398, 452, 484, 1640 |
| Tru1I | TTAA | 6 cut(s) | 548, 644, 693, 900, 1280, 1667 |
| Tru9I | TTAA | 6 cut(s) | 548, 644, 693, 900, 1280, 1667 |
| TscAI | CASTG | 4 cut(s) | 193, 685, 1123, 1544 |
| TseFI | GTSAC | 2 cut(s) | 862, 951 |
| TseI | GCWGC | 4 cut(s) | 191, 194, 776, 1004 |
| Tsp45I | GTSAC | 2 cut(s) | 862, 951 |
| TspGWI | ACGGA | 1 cut(s) | 717 |
| TspRI | CASTG | 4 cut(s) | 193, 685, 1123, 1544 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 474 |
| VpaK11BI | GGWCC | 2 cut(s) | 233, 1286 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 478 |
| XapI | RAATTY | 5 cut(s) | 155, 1017, 1349, 1401, 1660 |
| XceI | RCATGY | 1 cut(s) | 385 |
| XmiI | GTMKAC | 1 cut(s) | 1605 |
| XmnI | GAANNNNTTC | 2 cut(s) | 665, 1470 |
| XspI | CTAG | 1 cut(s) | 480 |
| Zsp2I | ATGCAT | 2 cut(s) | 121, 1472 |