Rw7G009740

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000392
Physical Location & Seq
Forward (+)
9057728 .. 9060300
2573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G009740.1

Sequence Viewer

Length: 1476 bp
ATGAATGCATCAGACCCTGAAGAATTGAACGTGAAGTGGACTGATTTCCCTAATGATTTTGTTTTTGGAGTCAGCACTGCTGCTACACAGATTGAAGGATCGGCAAAAGAAGCAGGAAAAGGACCAAGTGTTTGGGACAAGTTTGTAGAGATCCCTGAGAGAATTATGGACAAATCTAACATGTTTACGAGCATTGATTCATACAAGCGATACAAGGAAGATGTGAAGTTAGTGAAAGACCTTGGAGTTGATTCTTATAGATTTTCCATCCCCTGGACTAGGATTCTTCCGAATGGAACATTGAGTGGAGGAGTAAACCAAGAGGGTGTTGATCACTACAACTGTTTAATTGATGAACTAATCAAGAATGGCATCACACCCTATGTAACCCTATTACATTTTGATCCACCACAAGCCTTGACAGACAAGTATGATGGCGTTTTAAATCGTTCCTTTGTGAATGATTTCAAGGATTACAGTGAACTTTGTTTTAAGACCTACGGAGATAGGGTAAAAAATTGGATTACAATCAACGAGCCACTTGTAATGGCCAAAATGGGTTATGATCTTGCTGTTGCTCCACCAGGCAGGTGTTCAGTACAACTACCAAAAAGCAAGATTGGATGCAAAGTTGGTAATTCATCTACCGAACCTTACATTGTGACCCATAACCTTATCCTCTCTCATGCTACTGTTGTTAAGCTCTATAGAGAGAACTTTCAGGCAAAACAAGGTGGACAAATTGGATGGTCTCATGTAGGTCAGTATGTTGAGCCTTATTCAGATACAGAAGAAGACAAAGCTGCAGCAAAAAGAATTTTAGACTTTGAACTTGGATGGTTCATGGAACCAGTAGTATTCGGAGATTACCCAAAGATTATGAGAGATTTGGTCAAGGAAAGGCTACCCCTTTTCACTGAAGAAGAGAAAAAATTGATCAAGGGATCCTTCGATTTCATTGGCATCAACTATTATACCACGAGATATGGTAAAAACGTTCCAGCAAGTCAAGCTGAACCATTGAGTTATCACAATGACGCTTTGGCTACATCTACTGAAACAGATGCAAATGGAGTCTTAATTGGTCCTCTTGCTAATGGAAGCGTCTACATCTTCTCTTATCCACAAGGTCTTCAGAAACTTTTGGAGTTCATGAAGCAAAACTACCAAAGCCCTAAAATCTACATTTCCGAAAATGGAATTACAGAAGAAAAGGACGATAATCTTGGACTCGATGTAGCAGTCAAGGATCCACATAGAATTGAATGCATTCTTCGACACTTGTACAGGATCAAGATGGCAATGAAGAATGGGGTGAATGTCAAAGGATATTTCCACTGGACTCCATTCGATGACTTTGAGTGGGGGGAAGGCTATCTTCCAAGGAGCGGACTTTACTATGTCGACTACAAAGACAATCTCAAGCGCATTCCTAAAGAATCTGCTAAGTGGCTACCAAATTTCTTAAAGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

491

Amino Acids

55.9

Weight (kDa)

5.73

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 15 - 488 1.5e-145 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25630 AT2G44450 AT2G44450 AT3G60130 AT3G60130 AT3G60130 AT5G42260 AT5G44640
fragaria_vesca FvH4_5g07160 FvH4_5g07180 FvH4_5g07811 FvH4_5g07831 FvH4_5g07831 FvH4_5g07832 FvH4_6g19950 FvH4_6g19950 FvH4_6g19950
malus_domestica MD01G1120900.v1.1 MD06G1144600.v1.1 MD06G1144800.v1.1 MD06G1145700.v1.1 MD06G1146100.v1.1 MD06G1147000.v1.1 MD08G1072600.v1.1 MD08G1142300.v1.1 MD08G1191100.v1.1 MD14G1159400.v1.1 MD14G1160300.v1.1 MD14G1160600.v1.1 MD14G1160700.v1.1 MD14G1161400.v1.1
prunus_persica Prupe.1G525400_v2.0.a1 Prupe.1G583900_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G156500_v2.0.a1
pyrus_communis pycom06g13390 pycom06g13520 pycom06g13560 pycom06g13640 pycom08g12030 pycom14g13310 pycom14g13430
rosa_chinensis RchiOBHm_Chr3g0475401 RchiOBHm_Chr7g0190551 RchiOBHm_Chr7g0190631 RchiOBHm_Chr7g0190641 RchiOBHm_Chr7g0191241 RchiOBHm_Chr7g0191251 RchiOBHm_Chr7g0191261 RchiOBHm_Chr7g0202591
rosa_laevigata RLG00000004507 RLG00000004508 RLG00000004516 RLG00000023846 RLG00000035079
rosa_multiflora Rmu_co8005672.1_g000001 Rmu_co8018538.1_g000001 Rmu_sc0000526.1_g000002 Rmu_sc0001786.1_g000011 Rmu_sc0006709.1_g000007 Rmu_sc0006709.1_g000008 Rmu_sc0016637.1_g000004
rosa_roxburghii Rroxscaffold_1G00018560 Rroxscaffold_1G00024050 Rroxscaffold_3G00264440 Rroxscaffold_3G00264450 Rroxscaffold_4G00306640 Rroxscaffold_6G00406340
rosa_rugosa Rorug03G0295600 Rorug05G0298500 Rorug06G0072900 Rorug06G0505100 Rorug06G0505800 Rorug06G0505900 Rorug06G0506000
rosa_samantha Rh1CG344200 Rh2AG107900 Rh3AG198700 Rh3CG223000 Rh5AG369500 Rh5BG469700 Rh5BG469800 Rh5DG483000 Rh7AG111700 Rh7AG112400 Rh7AG112500 Rh7AG112600 Rh7CG116400 Rh7CG117400 Rh7CG117500 Rh7CG117600 Rh7CG122100 Rh7CG122200 Rh7DG115600 Rh7DG116400 Rh7DG116500
rosa_wichuraiana Rw3G018030 Rw7G009740 Rw7G009810 Rw7G009820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 579
Acc36I ACCTGC 1 cut(s) 579
AccB7I CCANNNNNTGG 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 1389
AccI GTMKAC 2 cut(s) 1107, 1404
AciI CCGC 1 cut(s) 1389
AclI AACGTT 1 cut(s) 996
AclWI GGATC 8 cut(s) 106, 145, 398, 939, 952, 1244, 1257, 1298
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 2 cut(s) 816, 1459
AcuI CTGAAG 3 cut(s) 39, 939, 1118
AfaI GTAC 2 cut(s) 600, 1286
AfiI CCNNNNNNNGG 3 cut(s) 273, 279, 1388
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 5 cut(s) 28, 95, 469, 830, 1265
AjnI CCWGG 2 cut(s) 272, 583
AluBI AGCT 3 cut(s) 703, 803, 1013
AluI AGCT 3 cut(s) 703, 803, 1013
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 8 cut(s) 106, 145, 398, 939, 952, 1244, 1257, 1298
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 80, 803, 806
ApoI RAATTY 2 cut(s) 816, 1459
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
Asp700I GAANNNNTTC 2 cut(s) 464, 1269
AspLEI GCGC 1 cut(s) 1428
AspS9I GGNCC 2 cut(s) 122, 1085
AsuHPI GGTGA 1 cut(s) 1327
AvaII GGWCC 2 cut(s) 122, 1085
BalI TGGCCA 1 cut(s) 551
BamHI GGATCC 2 cut(s) 944, 1249
BarI GAAGNNNNNNTAC 2 cut(s) 1148, 1180
BauI CACGAG 1 cut(s) 979
BbsI GAAGAC 2 cut(s) 801, 1124
BbvI GCAGC 3 cut(s) 67, 790, 818
BccI CCATC 5 cut(s) 275, 428, 741, 831, 1291
BciT130I CCWGG 2 cut(s) 274, 585
BclI TGATCA 2 cut(s) 331, 936
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 2 cut(s) 706, 804
BfuAI ACCTGC 1 cut(s) 579
BisI GCNGC 3 cut(s) 81, 804, 807
BlsI GCNGC 3 cut(s) 82, 805, 808
Bme1390I CCNGG 2 cut(s) 274, 585
Bme18I GGWCC 2 cut(s) 122, 1085
BmgT120I GGNCC 2 cut(s) 122, 1085
BmiI GGNNCC 3 cut(s) 849, 946, 1251
BmrFI CCNGG 2 cut(s) 274, 585
BmsI GCATC 5 cut(s) 17, 381, 614, 972, 1054
BpiI GAAGAC 2 cut(s) 801, 1124
BpuEI CTTGAG 1 cut(s) 1406
BsaBI GATNNNNATC 1 cut(s) 527
BsaI GGTCTC 1 cut(s) 756
BsaJI CCNNGG 3 cut(s) 241, 272, 1382
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 279, 1388
Bse1I ACTGG 2 cut(s) 851, 1343
Bse3DI GCAATG 1 cut(s) 1308
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 2 cut(s) 274, 585
BseDI CCNNGG 3 cut(s) 241, 272, 1382
BseGI GGATG 4 cut(s) 267, 629, 752, 842
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 3 cut(s) 273, 279, 1388
BseMI GCAATG 1 cut(s) 1308
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 2 cut(s) 851, 1343
BseRI GAGGAG 1 cut(s) 324
BseXI GCAGC 3 cut(s) 67, 790, 818
BshFI GGCC 1 cut(s) 551
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 273, 279, 1388
BsmAI GTCTC 1 cut(s) 756
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 4 cut(s) 10, 1269, 1271, 1428
BsnI GGCC 1 cut(s) 551
Bso31I GGTCTC 1 cut(s) 756
Bsp1407I TGTACA 1 cut(s) 1284
Bsp143I GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
BspACI CCGC 1 cut(s) 1389
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 3 cut(s) 849, 946, 1251
BspMAI CTGCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 579
BspPI GGATC 8 cut(s) 106, 145, 398, 939, 952, 1244, 1257, 1298
BspTNI GGTCTC 1 cut(s) 756
BsrBI CCGCTC 1 cut(s) 1389
BsrDI GCAATG 1 cut(s) 1308
BsrGI TGTACA 1 cut(s) 1284
BsrI ACTGG 2 cut(s) 851, 1343
BssECI CCNNGG 3 cut(s) 241, 272, 1382
BssMI GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
BssSI CACGAG 1 cut(s) 979
BssT1I CCWWGG 2 cut(s) 241, 1382
Bst2BI CACGAG 1 cut(s) 979
Bst2UI CCWGG 2 cut(s) 274, 585
Bst4CI ACNGT 3 cut(s) 344, 479, 694
Bst6I CTCTTC 1 cut(s) 918
BstAUI TGTACA 1 cut(s) 1284
BstDEI CTNAG 2 cut(s) 156, 1446
BstENI CCTNNNNNAGG 1 cut(s) 277
BstF5I GGATG 4 cut(s) 267, 629, 752, 842
BstHHI GCGC 1 cut(s) 1428
BstKTI GATC 9 cut(s) 101, 153, 334, 406, 568, 939, 947, 1252, 1293
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
BstMWI GCNNNNNNNGC 2 cut(s) 110, 1010
BstNI CCWGG 2 cut(s) 274, 585
BstNSI RCATGY 1 cut(s) 184
BstSCI CCNGG 2 cut(s) 272, 583
BstSFI CTRYAG 2 cut(s) 706, 804
BstV1I GCAGC 3 cut(s) 67, 790, 818
BstV2I GAAGAC 2 cut(s) 801, 1124
BstX2I RGATCY 3 cut(s) 150, 944, 1249
BstXI CCANNNNNNTGG 1 cut(s) 132
BstYI RGATCY 3 cut(s) 150, 944, 1249
BsuRI GGCC 1 cut(s) 551
BtsCI GGATG 4 cut(s) 267, 629, 752, 842
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 4 cut(s) 75, 484, 915, 1336
BveI ACCTGC 1 cut(s) 579
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 1 cut(s) 1152
CfoI GCGC 1 cut(s) 1428
Cfr13I GGNCC 2 cut(s) 122, 1085
CseI GACGC 2 cut(s) 1046, 1093
Csp6I GTAC 2 cut(s) 599, 1285
CspCI CAANNNNNGTGG 2 cut(s) 399, 434
CviAII CATG 5 cut(s) 181, 686, 755, 844, 1153
CviQI GTAC 2 cut(s) 599, 1285
DdeI CTNAG 2 cut(s) 156, 1446
DpnI GATC 9 cut(s) 100, 152, 333, 405, 567, 938, 946, 1251, 1292
DpnII GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
DraI TTTAAA 1 cut(s) 444
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
Eco130I CCWWGG 2 cut(s) 241, 1382
Eco31I GGTCTC 1 cut(s) 756
Eco47I GGWCC 2 cut(s) 122, 1085
Eco57I CTGAAG 3 cut(s) 39, 939, 1118
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRII CCWGG 2 cut(s) 272, 583
EcoT14I CCWWGG 2 cut(s) 241, 1382
EcoT22I ATGCAT 2 cut(s) 10, 1271
ErhI CCWWGG 2 cut(s) 241, 1382
FaeI CATG 5 cut(s) 184, 689, 758, 847, 1156
FalI AAGNNNNNCTT 4 cut(s) 932, 964, 1362, 1394
FaqI GGGAC 1 cut(s) 149
FatI CATG 5 cut(s) 180, 685, 754, 843, 1152
FbaI TGATCA 2 cut(s) 331, 936
FblI GTMKAC 2 cut(s) 1107, 1404
Fnu4HI GCNGC 3 cut(s) 81, 804, 807
FokI GGATG 4 cut(s) 254, 636, 759, 849
Fsp4HI GCNGC 3 cut(s) 81, 804, 807
FspBI CTAG 1 cut(s) 279
GlaI GCGC 1 cut(s) 1427
GluI GCNGC 3 cut(s) 81, 804, 807
HaeIII GGCC 1 cut(s) 551
HgaI GACGC 2 cut(s) 1046, 1093
HhaI GCGC 1 cut(s) 1428
Hin1II CATG 5 cut(s) 184, 689, 758, 847, 1156
Hin6I GCGC 1 cut(s) 1426
HinP1I GCGC 1 cut(s) 1426
HincII GTYRAC 1 cut(s) 1405
HindII GTYRAC 1 cut(s) 1405
HinfI GANTC 8 cut(s) 69, 197, 251, 283, 1074, 1230, 1342, 1439
HphI GGTGA 1 cut(s) 1327
Hpy166II GTNNAC 7 cut(s) 39, 186, 316, 482, 737, 1108, 1405
Hpy188I TCNGA 6 cut(s) 13, 291, 784, 863, 1137, 1192
Hpy188III TCNNGA 3 cut(s) 364, 1153, 1294
Hpy8I GTNNAC 7 cut(s) 39, 186, 316, 482, 737, 1108, 1405
HpyAV CCTTC 3 cut(s) 89, 958, 1364
HpyCH4III ACNGT 3 cut(s) 344, 479, 694
HpyCH4IV ACGT 2 cut(s) 30, 996
HpyCH4V TGCA 5 cut(s) 8, 627, 806, 1067, 1269
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 1010
HpyF3I CTNAG 2 cut(s) 156, 1446
HpySE526I ACGT 2 cut(s) 30, 996
Hsp92II CATG 5 cut(s) 184, 689, 758, 847, 1156
HspAI GCGC 1 cut(s) 1426
Ksp22I TGATCA 2 cut(s) 331, 936
Kzo9I GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
LmnI GCTCC 2 cut(s) 583, 1386
Lsp1109I GCAGC 3 cut(s) 67, 790, 818
LweI GCATC 5 cut(s) 17, 381, 614, 972, 1054
MaeI CTAG 1 cut(s) 279
MaeII ACGT 2 cut(s) 30, 996
MaeIII GTNAC 2 cut(s) 385, 661
MalI GATC 9 cut(s) 100, 152, 333, 405, 567, 938, 946, 1251, 1292
MbiI CCGCTC 1 cut(s) 1389
MboI GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
MflI RGATCY 3 cut(s) 150, 944, 1249
MlsI TGGCCA 1 cut(s) 551
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 4 cut(s) 78, 1083, 1224, 1336
MnlI CCTC 4 cut(s) 302, 316, 689, 1098
Mox20I TGGCCA 1 cut(s) 551
Mph1103I ATGCAT 2 cut(s) 10, 1271
MroXI GAANNNNTTC 2 cut(s) 464, 1269
MscI TGGCCA 1 cut(s) 551
MseI TTAA 6 cut(s) 347, 443, 492, 699, 1079, 1466
Msp20I TGGCCA 1 cut(s) 551
MspR9I CCNGG 2 cut(s) 274, 585
Mva1269I GAATGC 4 cut(s) 10, 1269, 1271, 1428
MvaI CCWGG 2 cut(s) 274, 585
MwoI GCNNNNNNNGC 2 cut(s) 110, 1010
NdeII GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
NlaIII CATG 5 cut(s) 184, 689, 758, 847, 1156
NlaIV GGNNCC 3 cut(s) 849, 946, 1251
NmuCI GTSAC 1 cut(s) 661
NsiI ATGCAT 2 cut(s) 10, 1271
NspI RCATGY 1 cut(s) 184
PagI TCATGA 1 cut(s) 1152
PaqCI CACCTGC 1 cut(s) 579
PciI ACATGT 1 cut(s) 180
PctI GAATGC 4 cut(s) 10, 1269, 1271, 1428
PdmI GAANNNNTTC 2 cut(s) 464, 1269
PfeI GAWTC 4 cut(s) 197, 251, 283, 1439
PflMI CCANNNNNTGG 1 cut(s) 273
PkrI GCNGC 3 cut(s) 82, 805, 808
PleI GAGTC 4 cut(s) 77, 1082, 1224, 1336
PpsI GAGTC 4 cut(s) 77, 1082, 1224, 1336
PscI ACATGT 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 996
Psp6I CCWGG 2 cut(s) 272, 583
PspGI CCWGG 2 cut(s) 272, 583
PspN4I GGNNCC 3 cut(s) 849, 946, 1251
PspPI GGNCC 2 cut(s) 122, 1085
PstI CTGCAG 1 cut(s) 808
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 3 cut(s) 150, 944, 1249
RsaI GTAC 2 cut(s) 600, 1286
RsaNI GTAC 2 cut(s) 599, 1285
SalI GTCGAC 1 cut(s) 1403
SaqAI TTAA 6 cut(s) 347, 443, 492, 699, 1079, 1466
SatI GCNGC 3 cut(s) 81, 804, 807
Sau3AI GATC 9 cut(s) 98, 150, 331, 403, 565, 936, 944, 1249, 1290
Sau96I GGNCC 2 cut(s) 122, 1085
SchI GAGTC 4 cut(s) 78, 1083, 1224, 1336
ScrFI CCNGG 2 cut(s) 274, 585
SfaNI GCATC 5 cut(s) 17, 381, 614, 972, 1054
SfcI CTRYAG 2 cut(s) 706, 804
SinI GGWCC 2 cut(s) 122, 1085
SmlI CTYRAG 1 cut(s) 1421
SmoI CTYRAG 1 cut(s) 1421
SsiI CCGC 1 cut(s) 1389
SspMI CTAG 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 272, 583
StyI CCWWGG 2 cut(s) 241, 1382
TaaI ACNGT 3 cut(s) 344, 479, 694
TaiI ACGT 2 cut(s) 33, 999
TaqI TCGA 5 cut(s) 951, 1233, 1276, 1350, 1404
TatI WGTACW 2 cut(s) 598, 1284
TfiI GAWTC 4 cut(s) 197, 251, 283, 1439
Tru1I TTAA 6 cut(s) 347, 443, 492, 699, 1079, 1466
Tru9I TTAA 6 cut(s) 347, 443, 492, 699, 1079, 1466
TscAI CASTG 4 cut(s) 82, 484, 922, 1343
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 3 cut(s) 80, 803, 806
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 9 cut(s) 17, 189, 369, 630, 832, 946, 1141, 1169, 1319
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 4 cut(s) 82, 484, 922, 1343
Van91I CCANNNNNTGG 1 cut(s) 273
VpaK11BI GGWCC 2 cut(s) 122, 1085
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 816, 1459
XceI RCATGY 1 cut(s) 184
XmiI GTMKAC 2 cut(s) 1107, 1404
XmnI GAANNNNTTC 2 cut(s) 464, 1269
XspI CTAG 1 cut(s) 279
Zsp2I ATGCAT 2 cut(s) 10, 1271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.