Rh3AG198700

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000392
Physical Location & Seq
Forward (+)
19451338 .. 19454462
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG198700.1

Sequence Viewer

Length: 1230 bp
ATGGAGTTTTCACCAACATGGTTAAGGATTTCACAGAGGATGATGTGTGCTATTGCTTTCATTGCCTGTTTTTCACCAGTTACGGTGAATGCTAAGATAAAGTTCCAGGCATTGCACATCAATATATCAAACCCTAAAGAATTAAAAGTGAAGTGGTCCGACTTCCCCAATGATTTTGCATTTGGAGTCAGCACTGCTGCTGCACAGATTGAAGGATCAACAAATAAAGCAGGACGAGGACCAAGTGTTTGGGACGAGTTTGTTAAGACATTCCCAGAACGCATTGCAGACCATTCTACCATGTCTACCGCAATCGACTCATACAAGCGATACAAGGAAGATGTGAAGGCAGTGAAAGCCCTTGGAGTTGATTCTTATAGATTTTCCATCTCTTGGACCAGGATTCTCCCCAAGGGAACCTTGAGTGGAGGAATAAACCAAGAGGGTATTGATCACTACAACAACTTGATTGATGAACTAATCAAGAATGGTTCCTATGTTGAGCCTTATTCAAATTCATCTGAGGACAAAGCTGCAGCAAAAAGAATTTTGGACTTTGAACTTGGATGGTTCATGGAACCAATAGTATATGGACATTATCCAAAGAGTATGAGGCGCTTGGTCAGGAAAAGGCTACCCCGTTTCACCAAAGAAGAGAAGAAATTGATTAAAGGATCCTTTGATTTCATTGGCATCAACTATTATACCTCGAGATATGGTAGAAACATTCCAGCACGTCTAGATATGCCAAACTTCTATCGCTATGATCCTCTAGCGTCATCAGAGCGCAAGAATGTAAATGGAGTCCCAATTGGTCCTCAGGCTGATGGTAGCATGTTCATCTTGTCTTATCCACAAGGTCTCAAGAAACTTTTGAAGTTCATGAAGCGAAAATACCAGAGCCCTAAGATCTACATTTCCGAAAATGGAATTACAGAGGCAAAGGACGATAAGCGTGGACTTGATGTAGCACTGAAGGATCCACATAGAATTGAATGTATTCTTCGGCATTTGTACATGATCAAGCAGGCCATAAAGAAGGGTGTGAACGTCAAAGGATATTTCCACTGGGCTTTATTTGACGATTTTGAGTGGGGGGAAGGCTATATCCCAAGGTACGGGCTTTATTATGTCGACTACAATGACAATCTCAAGCGCATTCCTAAGGAATCTGCAAAGTGGCTCCCTAAATTCCTAAAAGGCGAAGATGCGATACATGTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

409

Amino Acids

47.14

Weight (kDa)

9.51

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 54 - 164 1.8e-41 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 164 - 400 3.6e-61 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25630 AT2G44450 AT2G44450 AT3G60130 AT3G60130 AT3G60130 AT5G42260 AT5G44640
fragaria_vesca FvH4_5g07160 FvH4_5g07180 FvH4_5g07811 FvH4_5g07831 FvH4_5g07831 FvH4_5g07832 FvH4_6g19950 FvH4_6g19950 FvH4_6g19950
malus_domestica MD01G1120900.v1.1 MD06G1144600.v1.1 MD06G1144800.v1.1 MD06G1145700.v1.1 MD06G1146100.v1.1 MD06G1147000.v1.1 MD08G1072600.v1.1 MD08G1142300.v1.1 MD08G1191100.v1.1 MD14G1159400.v1.1 MD14G1160300.v1.1 MD14G1160600.v1.1 MD14G1160700.v1.1 MD14G1161400.v1.1
prunus_persica Prupe.1G525400_v2.0.a1 Prupe.1G583900_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G156500_v2.0.a1
pyrus_communis pycom06g13390 pycom06g13520 pycom06g13560 pycom06g13640 pycom08g12030 pycom14g13310 pycom14g13430
rosa_chinensis RchiOBHm_Chr3g0475401 RchiOBHm_Chr7g0190551 RchiOBHm_Chr7g0190631 RchiOBHm_Chr7g0190641 RchiOBHm_Chr7g0191241 RchiOBHm_Chr7g0191251 RchiOBHm_Chr7g0191261 RchiOBHm_Chr7g0202591
rosa_laevigata RLG00000004507 RLG00000004508 RLG00000004516 RLG00000023846 RLG00000035079
rosa_multiflora Rmu_co8005672.1_g000001 Rmu_co8018538.1_g000001 Rmu_sc0000526.1_g000002 Rmu_sc0001786.1_g000011 Rmu_sc0006709.1_g000007 Rmu_sc0006709.1_g000008 Rmu_sc0016637.1_g000004
rosa_roxburghii Rroxscaffold_1G00018560 Rroxscaffold_1G00024050 Rroxscaffold_3G00264440 Rroxscaffold_3G00264450 Rroxscaffold_4G00306640 Rroxscaffold_6G00406340
rosa_rugosa Rorug03G0295600 Rorug05G0298500 Rorug06G0072900 Rorug06G0505100 Rorug06G0505800 Rorug06G0505900 Rorug06G0506000
rosa_samantha Rh1CG344200 Rh2AG107900 Rh3AG198700 Rh3CG223000 Rh5AG369500 Rh5BG469700 Rh5BG469800 Rh5DG483000 Rh7AG111700 Rh7AG112400 Rh7AG112500 Rh7AG112600 Rh7CG116400 Rh7CG117400 Rh7CG117500 Rh7CG117600 Rh7CG122100 Rh7CG122200 Rh7DG115600 Rh7DG116400 Rh7DG116500
rosa_wichuraiana Rw3G018030 Rw7G009740 Rw7G009810 Rw7G009820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 393
AccI GTMKAC 2 cut(s) 305, 1134
AciI CCGC 1 cut(s) 309
AclWI GGATC 6 cut(s) 223, 669, 682, 761, 974, 987
AcsI RAATTY 3 cut(s) 514, 546, 1190
AcuI CTGAAG 1 cut(s) 995
AfaI GTAC 2 cut(s) 1016, 1118
AfiI CCNNNNNNNGG 2 cut(s) 393, 1118
AflIII ACRYGT 1 cut(s) 1216
AgsI TTSAA 5 cut(s) 212, 513, 560, 877, 995
AjiI CACGTC 1 cut(s) 737
AjnI CCWGG 2 cut(s) 105, 398
AluBI AGCT 2 cut(s) 533, 1226
AluI AGCT 2 cut(s) 533, 1226
Alw26I GTCTC 1 cut(s) 866
AlwI GGATC 6 cut(s) 223, 669, 682, 761, 974, 987
Ama87I CYCGRG 1 cut(s) 709
AoxI GGCC 1 cut(s) 1029
ApeKI GCWGC 4 cut(s) 197, 200, 533, 536
ApoI RAATTY 3 cut(s) 514, 546, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
Asp700I GAANNNNTTC 1 cut(s) 999
AspLEI GCGC 3 cut(s) 618, 789, 1158
AspS9I GGNCC 4 cut(s) 156, 239, 396, 815
AsuHPI GGTGA 4 cut(s) 3, 66, 97, 637
AvaI CYCGRG 1 cut(s) 709
AvaII GGWCC 4 cut(s) 156, 239, 396, 815
AxyI CCTNAGG 2 cut(s) 819, 1164
BamHI GGATCC 2 cut(s) 674, 979
BanII GRGCYC 1 cut(s) 905
BarI GAAGNNNNNNTAC 4 cut(s) 878, 910, 1197, 1229
BbvI GCAGC 4 cut(s) 184, 187, 520, 548
BccI CCATC 3 cut(s) 395, 561, 821
BciT130I CCWGG 2 cut(s) 107, 400
BclI TGATCA 2 cut(s) 451, 1020
BcoDI GTCTC 1 cut(s) 866
BfaI CTAG 2 cut(s) 740, 773
BfmI CTRYAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 619
BglII AGATCT 1 cut(s) 909
BisI GCNGC 4 cut(s) 198, 201, 534, 537
BlsI GCNGC 4 cut(s) 199, 202, 535, 538
Bme1390I CCNGG 2 cut(s) 107, 400
Bme18I GGWCC 4 cut(s) 156, 239, 396, 815
BmeT110I CYCGRG 1 cut(s) 709
BmgBI CACGTC 1 cut(s) 737
BmgT120I GGNCC 4 cut(s) 156, 239, 396, 815
BmiI GGNNCC 6 cut(s) 418, 493, 579, 676, 981, 1184
BmrFI CCNGG 2 cut(s) 107, 400
BmrI ACTGGG 1 cut(s) 1078
BmsI GCATC 2 cut(s) 702, 1198
BmuI ACTGGG 1 cut(s) 1078
BpuEI CTTGAG 3 cut(s) 442, 848, 1136
BsaI GGTCTC 1 cut(s) 866
BsaJI CCNNGG 3 cut(s) 361, 411, 1112
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 1118
Bse1I ACTGG 2 cut(s) 77, 1073
Bse21I CCTNAGG 2 cut(s) 819, 1164
Bse3DI GCAATG 3 cut(s) 60, 110, 282
BseBI CCWGG 2 cut(s) 107, 400
BseDI CCNNGG 3 cut(s) 361, 411, 1112
BseGI GGATG 2 cut(s) 45, 572
BseLI CCNNNNNNNGG 2 cut(s) 393, 1118
BseMI GCAATG 3 cut(s) 60, 110, 282
BseMII CTCAG 2 cut(s) 513, 833
BseNI ACTGG 2 cut(s) 77, 1073
BseXI GCAGC 4 cut(s) 184, 187, 520, 548
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 1 cut(s) 1031
BsiHKCI CYCGRG 1 cut(s) 709
BslFI GGGAC 2 cut(s) 266, 791
BslI CCNNNNNNNGG 2 cut(s) 393, 1118
BsmAI GTCTC 1 cut(s) 866
BsmFI GGGAC 2 cut(s) 266, 791
BsmI GAATGC 2 cut(s) 94, 1158
BsnI GGCC 1 cut(s) 1031
Bso31I GGTCTC 1 cut(s) 866
BsoBI CYCGRG 1 cut(s) 709
Bsp1286I GDGCHC 1 cut(s) 905
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
BspACI CCGC 1 cut(s) 309
BspANI GGCC 1 cut(s) 1031
BspCNI CTCAG 2 cut(s) 514, 832
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 6 cut(s) 418, 493, 579, 676, 981, 1184
BspMAI CTGCAG 1 cut(s) 538
BspPI GGATC 6 cut(s) 223, 669, 682, 761, 974, 987
BspTNI GGTCTC 1 cut(s) 866
BsrDI GCAATG 3 cut(s) 60, 110, 282
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 2 cut(s) 77, 1073
BssECI CCNNGG 3 cut(s) 361, 411, 1112
BssMI GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
BssT1I CCWWGG 3 cut(s) 361, 411, 1112
Bst2UI CCWGG 2 cut(s) 107, 400
Bst4CI ACNGT 1 cut(s) 85
Bst6I CTCTTC 1 cut(s) 648
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 1 cut(s) 1029
BstDEI CTNAG 6 cut(s) 93, 522, 819, 906, 1164, 1227
BstF5I GGATG 2 cut(s) 45, 572
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 3 cut(s) 618, 789, 1158
BstKTI GATC 7 cut(s) 218, 454, 677, 769, 912, 982, 1023
BstMAI GTCTC 1 cut(s) 866
BstMBI GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
BstMWI GCNNNNNNNGC 2 cut(s) 62, 356
BstNI CCWGG 2 cut(s) 107, 400
BstNSI RCATGY 2 cut(s) 838, 1220
BstSCI CCNGG 2 cut(s) 105, 398
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 4 cut(s) 184, 187, 520, 548
BstX2I RGATCY 3 cut(s) 674, 909, 979
BstXI CCANNNNNNTGG 1 cut(s) 249
BstYI RGATCY 3 cut(s) 674, 909, 979
Bsu36I CCTNAGG 2 cut(s) 819, 1164
BsuRI GGCC 1 cut(s) 1031
BtrI CACGTC 1 cut(s) 737
BtsCI GGATG 2 cut(s) 45, 572
BtsI GCAGTG 2 cut(s) 192, 357
BtsIMutI CAGTG 4 cut(s) 192, 357, 971, 1066
Cac8I GCNNGC 1 cut(s) 1029
CciI TCATGA 1 cut(s) 882
CfoI GCGC 3 cut(s) 618, 789, 1158
Cfr13I GGNCC 4 cut(s) 156, 239, 396, 815
CseI GACGC 1 cut(s) 765
Csp6I GTAC 2 cut(s) 1015, 1117
CviAII CATG 7 cut(s) 18, 301, 574, 835, 883, 1018, 1217
CviQI GTAC 2 cut(s) 1015, 1117
DdeI CTNAG 6 cut(s) 93, 522, 819, 906, 1164, 1227
DpnI GATC 7 cut(s) 217, 453, 676, 768, 911, 981, 1022
DpnII GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Eco130I CCWWGG 3 cut(s) 361, 411, 1112
Eco24I GRGCYC 1 cut(s) 905
Eco31I GGTCTC 1 cut(s) 866
Eco47I GGWCC 4 cut(s) 156, 239, 396, 815
Eco57I CTGAAG 1 cut(s) 995
Eco81I CCTNAGG 2 cut(s) 819, 1164
Eco88I CYCGRG 1 cut(s) 709
EcoRII CCWGG 2 cut(s) 105, 398
EcoT14I CCWWGG 3 cut(s) 361, 411, 1112
EcoT38I GRGCYC 1 cut(s) 905
ErhI CCWWGG 3 cut(s) 361, 411, 1112
FaeI CATG 7 cut(s) 21, 304, 577, 838, 886, 1021, 1220
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FaqI GGGAC 2 cut(s) 266, 791
FatI CATG 7 cut(s) 17, 300, 573, 834, 882, 1017, 1216
FbaI TGATCA 2 cut(s) 451, 1020
FblI GTMKAC 2 cut(s) 305, 1134
Fnu4HI GCNGC 4 cut(s) 198, 201, 534, 537
FokI GGATG 2 cut(s) 52, 579
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 4 cut(s) 198, 201, 534, 537
FspBI CTAG 2 cut(s) 740, 773
GlaI GCGC 3 cut(s) 617, 788, 1157
GluI GCNGC 4 cut(s) 198, 201, 534, 537
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 1 cut(s) 1031
HgaI GACGC 1 cut(s) 765
HhaI GCGC 3 cut(s) 618, 789, 1158
Hin1II CATG 7 cut(s) 21, 304, 577, 838, 886, 1021, 1220
Hin6I GCGC 3 cut(s) 616, 787, 1156
HinP1I GCGC 3 cut(s) 616, 787, 1156
HincII GTYRAC 1 cut(s) 1135
HindII GTYRAC 1 cut(s) 1135
HindIII AAGCTT 1 cut(s) 1224
HinfI GANTC 6 cut(s) 186, 317, 371, 403, 804, 1169
HphI GGTGA 4 cut(s) 3, 66, 97, 637
Hpy166II GTNNAC 4 cut(s) 306, 959, 1048, 1135
Hpy188I TCNGA 4 cut(s) 160, 523, 784, 922
Hpy188III TCNNGA 6 cut(s) 484, 625, 711, 740, 865, 883
Hpy8I GTNNAC 4 cut(s) 306, 959, 1048, 1135
HpyAV CCTTC 5 cut(s) 206, 340, 970, 1033, 1094
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 2 cut(s) 736, 1050
HpyCH4V TGCA 6 cut(s) 115, 179, 203, 287, 536, 1175
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 356
HpyF3I CTNAG 6 cut(s) 93, 522, 819, 906, 1164, 1227
HpySE526I ACGT 2 cut(s) 736, 1050
Hsp92II CATG 7 cut(s) 21, 304, 577, 838, 886, 1021, 1220
HspAI GCGC 3 cut(s) 616, 787, 1156
Ksp22I TGATCA 2 cut(s) 451, 1020
Kzo9I GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
LmnI GCTCC 1 cut(s) 1188
Lsp1109I GCAGC 4 cut(s) 184, 187, 520, 548
LweI GCATC 2 cut(s) 702, 1198
MaeI CTAG 2 cut(s) 740, 773
MaeII ACGT 2 cut(s) 736, 1050
MaeIII GTNAC 1 cut(s) 79
MalI GATC 7 cut(s) 217, 453, 676, 768, 911, 981, 1022
MboI GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
MboII GAAGA 5 cut(s) 350, 665, 670, 995, 1217
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 3 cut(s) 674, 909, 979
MhlI GDGCHC 1 cut(s) 905
MluCI AATT 8 cut(s) 140, 514, 546, 662, 810, 930, 990, 1190
MlyI GAGTC 3 cut(s) 195, 311, 813
MmeI TCCRAC 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 999
MseI TTAA 4 cut(s) 23, 143, 264, 669
MslI CAYNNNNRTG 1 cut(s) 16
MspR9I CCNGG 2 cut(s) 107, 400
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 2 cut(s) 94, 1158
MvaI CCWGG 2 cut(s) 107, 400
MwoI GCNNNNNNNGC 2 cut(s) 62, 356
NdeII GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
NlaIII CATG 7 cut(s) 21, 304, 577, 838, 886, 1021, 1220
NlaIV GGNNCC 6 cut(s) 418, 493, 579, 676, 981, 1184
NspI RCATGY 2 cut(s) 838, 1220
PaeR7I CTCGAG 1 cut(s) 709
PagI TCATGA 1 cut(s) 882
PciI ACATGT 1 cut(s) 1216
PctI GAATGC 2 cut(s) 94, 1158
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 3 cut(s) 371, 403, 1169
PflMI CCANNNNNTGG 1 cut(s) 393
PkrI GCNGC 4 cut(s) 199, 202, 535, 538
PleI GAGTC 3 cut(s) 194, 311, 812
PpsI GAGTC 3 cut(s) 194, 311, 812
PscI ACATGT 1 cut(s) 1216
Psp6I CCWGG 2 cut(s) 105, 398
PspGI CCWGG 2 cut(s) 105, 398
PspN4I GGNNCC 6 cut(s) 418, 493, 579, 676, 981, 1184
PspPI GGNCC 4 cut(s) 156, 239, 396, 815
PstI CTGCAG 1 cut(s) 538
PsuI RGATCY 3 cut(s) 674, 909, 979
RsaI GTAC 2 cut(s) 1016, 1118
RsaNI GTAC 2 cut(s) 1015, 1117
RseI CAYNNNNRTG 1 cut(s) 16
SalI GTCGAC 1 cut(s) 1133
SaqAI TTAA 4 cut(s) 23, 143, 264, 669
SatI GCNGC 4 cut(s) 198, 201, 534, 537
Sau3AI GATC 7 cut(s) 215, 451, 674, 766, 909, 979, 1020
Sau96I GGNCC 4 cut(s) 156, 239, 396, 815
SchI GAGTC 3 cut(s) 195, 311, 813
ScrFI CCNGG 2 cut(s) 107, 400
SduI GDGCHC 1 cut(s) 905
SetI ASST 8 cut(s) 422, 535, 710, 739, 862, 1053, 1118, 1228
SfaNI GCATC 2 cut(s) 702, 1198
SfcI CTRYAG 1 cut(s) 534
Sfr274I CTCGAG 1 cut(s) 709
SinI GGWCC 4 cut(s) 156, 239, 396, 815
SlaI CTCGAG 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 16
SmlI CTYRAG 4 cut(s) 421, 709, 863, 1151
SmoI CTYRAG 4 cut(s) 421, 709, 863, 1151
Sse9I AATT 8 cut(s) 140, 514, 546, 662, 810, 930, 990, 1190
SsiI CCGC 1 cut(s) 309
SspMI CTAG 2 cut(s) 740, 773
StyD4I CCNGG 2 cut(s) 105, 398
StyI CCWWGG 3 cut(s) 361, 411, 1112
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 2 cut(s) 739, 1053
TaqI TCGA 3 cut(s) 315, 710, 1134
TasI AATT 8 cut(s) 140, 514, 546, 662, 810, 930, 990, 1190
TatI WGTACW 1 cut(s) 1014
TfiI GAWTC 3 cut(s) 371, 403, 1169
Tru1I TTAA 4 cut(s) 23, 143, 264, 669
Tru9I TTAA 4 cut(s) 23, 143, 264, 669
TscAI CASTG 4 cut(s) 199, 357, 978, 1073
TseI GCWGC 4 cut(s) 197, 200, 533, 536
TspDTI ATGAA 8 cut(s) 49, 489, 507, 562, 676, 829, 871, 899
TspRI CASTG 4 cut(s) 199, 357, 978, 1073
Van91I CCANNNNNTGG 1 cut(s) 393
VpaK11BI GGWCC 4 cut(s) 156, 239, 396, 815
XapI RAATTY 3 cut(s) 514, 546, 1190
XbaI TCTAGA 1 cut(s) 739
XceI RCATGY 2 cut(s) 838, 1220
XhoI CTCGAG 1 cut(s) 709
XmiI GTMKAC 2 cut(s) 305, 1134
XmnI GAANNNNTTC 1 cut(s) 999
XspI CTAG 2 cut(s) 740, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.