Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 759 |
| Acc36I | ACCTGC | 1 cut(s) | 759 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 363 |
| AccBSI | CCGCTC | 1 cut(s) | 1581 |
| AccI | GTMKAC | 3 cut(s) | 484, 1299, 1596 |
| AciI | CCGC | 1 cut(s) | 1581 |
| AclI | AACGTT | 1 cut(s) | 1176 |
| AclWI | GGATC | 8 cut(s) | 106, 145, 578, 1119, 1132, 1436, 1449, 1490 |
| AcoI | YGGCCR | 1 cut(s) | 729 |
| AcsI | RAATTY | 2 cut(s) | 996, 1651 |
| AcuI | CTGAAG | 3 cut(s) | 39, 1119, 1310 |
| AfaI | GTAC | 2 cut(s) | 780, 1478 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 363, 369, 1580 |
| AflIII | ACRYGT | 1 cut(s) | 270 |
| AgsI | TTSAA | 6 cut(s) | 28, 95, 649, 1010, 1421, 1457 |
| AjnI | CCWGG | 3 cut(s) | 154, 362, 763 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 216, 248, 513, 545 |
| AluBI | AGCT | 3 cut(s) | 883, 983, 1193 |
| AluI | AGCT | 3 cut(s) | 883, 983, 1193 |
| Alw26I | GTCTC | 2 cut(s) | 544, 936 |
| AlwI | GGATC | 8 cut(s) | 106, 145, 578, 1119, 1132, 1436, 1449, 1490 |
| AoxI | GGCC | 1 cut(s) | 729 |
| ApeKI | GCWGC | 4 cut(s) | 80, 83, 983, 986 |
| ApoI | RAATTY | 2 cut(s) | 996, 1651 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 114, 146 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 644, 1461 |
| AspLEI | GCGC | 1 cut(s) | 1620 |
| AspS9I | GGNCC | 2 cut(s) | 122, 1265 |
| AsuHPI | GGTGA | 1 cut(s) | 1519 |
| AvaII | GGWCC | 2 cut(s) | 122, 1265 |
| BalI | TGGCCA | 1 cut(s) | 731 |
| BamHI | GGATCC | 2 cut(s) | 1124, 1441 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1340, 1372 |
| BauI | CACGAG | 1 cut(s) | 1159 |
| BbsI | GAAGAC | 2 cut(s) | 981, 1316 |
| BbvI | GCAGC | 4 cut(s) | 67, 70, 970, 998 |
| BccI | CCATC | 6 cut(s) | 202, 365, 608, 921, 1011, 1483 |
| BciT130I | CCWGG | 3 cut(s) | 156, 364, 765 |
| BclI | TGATCA | 2 cut(s) | 421, 1116 |
| BcoDI | GTCTC | 2 cut(s) | 544, 936 |
| BfaI | CTAG | 1 cut(s) | 369 |
| BfmI | CTRYAG | 2 cut(s) | 886, 984 |
| BfuAI | ACCTGC | 1 cut(s) | 759 |
| BisI | GCNGC | 4 cut(s) | 81, 84, 984, 987 |
| BlsI | GCNGC | 4 cut(s) | 82, 85, 985, 988 |
| Bme1390I | CCNGG | 3 cut(s) | 156, 364, 765 |
| Bme18I | GGWCC | 2 cut(s) | 122, 1265 |
| BmgT120I | GGNCC | 2 cut(s) | 122, 1265 |
| BmiI | GGNNCC | 3 cut(s) | 1029, 1126, 1443 |
| BmrFI | CCNGG | 3 cut(s) | 156, 364, 765 |
| BmsI | GCATC | 4 cut(s) | 17, 561, 794, 1152 |
| BpiI | GAAGAC | 2 cut(s) | 981, 1316 |
| BpuEI | CTTGAG | 1 cut(s) | 1598 |
| BsaBI | GATNNNNATC | 1 cut(s) | 707 |
| BsaI | GGTCTC | 1 cut(s) | 936 |
| BsaJI | CCNNGG | 4 cut(s) | 154, 331, 362, 1574 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 675, 705 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 363, 369, 1580 |
| Bse1I | ACTGG | 1 cut(s) | 1535 |
| Bse3DI | GCAATG | 2 cut(s) | 473, 1500 |
| Bse8I | GATNNNNATC | 1 cut(s) | 707 |
| BseBI | CCWGG | 3 cut(s) | 156, 364, 765 |
| BseDI | CCNNGG | 4 cut(s) | 154, 331, 362, 1574 |
| BseGI | GGATG | 4 cut(s) | 357, 809, 932, 1022 |
| BseJI | GATNNNNATC | 1 cut(s) | 707 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 363, 369, 1580 |
| BseMI | GCAATG | 2 cut(s) | 473, 1500 |
| BseNI | ACTGG | 1 cut(s) | 1535 |
| BseRI | GAGGAG | 1 cut(s) | 414 |
| BseXI | GCAGC | 4 cut(s) | 67, 70, 970, 998 |
| BsgI | GTGCAG | 1 cut(s) | 69 |
| BshFI | GGCC | 1 cut(s) | 731 |
| BslFI | GGGAC | 2 cut(s) | 149, 183 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 363, 369, 1580 |
| BsmAI | GTCTC | 2 cut(s) | 544, 936 |
| BsmFI | GGGAC | 2 cut(s) | 149, 183 |
| BsmI | GAATGC | 4 cut(s) | 10, 1461, 1463, 1620 |
| BsnI | GGCC | 1 cut(s) | 731 |
| Bso31I | GGTCTC | 1 cut(s) | 936 |
| Bsp1407I | TGTACA | 1 cut(s) | 1476 |
| Bsp143I | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| BspACI | CCGC | 1 cut(s) | 1581 |
| BspANI | GGCC | 1 cut(s) | 731 |
| BspHI | TCATGA | 1 cut(s) | 1344 |
| BspLI | GGNNCC | 3 cut(s) | 1029, 1126, 1443 |
| BspMAI | CTGCAG | 1 cut(s) | 988 |
| BspMI | ACCTGC | 1 cut(s) | 759 |
| BspPI | GGATC | 8 cut(s) | 106, 145, 578, 1119, 1132, 1436, 1449, 1490 |
| BspTNI | GGTCTC | 1 cut(s) | 936 |
| BsrBI | CCGCTC | 1 cut(s) | 1581 |
| BsrDI | GCAATG | 2 cut(s) | 473, 1500 |
| BsrGI | TGTACA | 1 cut(s) | 1476 |
| BsrI | ACTGG | 1 cut(s) | 1535 |
| BssECI | CCNNGG | 4 cut(s) | 154, 331, 362, 1574 |
| BssMI | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| BssNAI | GTATAC | 1 cut(s) | 485 |
| BssSI | CACGAG | 1 cut(s) | 1159 |
| BssT1I | CCWWGG | 2 cut(s) | 331, 1574 |
| Bst1107I | GTATAC | 1 cut(s) | 485 |
| Bst2BI | CACGAG | 1 cut(s) | 1159 |
| Bst2UI | CCWGG | 3 cut(s) | 156, 364, 765 |
| Bst4CI | ACNGT | 3 cut(s) | 434, 659, 874 |
| Bst6I | CTCTTC | 1 cut(s) | 1098 |
| BstAUI | TGTACA | 1 cut(s) | 1476 |
| BstC8I | GCNNGC | 1 cut(s) | 550 |
| BstDEI | CTNAG | 1 cut(s) | 1638 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 367 |
| BstF5I | GGATG | 4 cut(s) | 357, 809, 932, 1022 |
| BstHHI | GCGC | 1 cut(s) | 1620 |
| BstKTI | GATC | 9 cut(s) | 101, 153, 424, 586, 748, 1119, 1127, 1444, 1485 |
| BstMAI | GTCTC | 2 cut(s) | 544, 936 |
| BstMBI | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 110, 1190 |
| BstNI | CCWGG | 3 cut(s) | 156, 364, 765 |
| BstNSI | RCATGY | 1 cut(s) | 274 |
| BstSCI | CCNGG | 3 cut(s) | 154, 362, 763 |
| BstSFI | CTRYAG | 2 cut(s) | 886, 984 |
| BstV1I | GCAGC | 4 cut(s) | 67, 70, 970, 998 |
| BstV2I | GAAGAC | 2 cut(s) | 981, 1316 |
| BstX2I | RGATCY | 3 cut(s) | 150, 1124, 1441 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 132 |
| BstYI | RGATCY | 3 cut(s) | 150, 1124, 1441 |
| BstZ17I | GTATAC | 1 cut(s) | 485 |
| BsuRI | GGCC | 1 cut(s) | 731 |
| BtsCI | GGATG | 4 cut(s) | 357, 809, 932, 1022 |
| BtsI | GCAGTG | 1 cut(s) | 75 |
| BtsIMutI | CAGTG | 4 cut(s) | 75, 664, 1095, 1528 |
| BveI | ACCTGC | 1 cut(s) | 759 |
| Cac8I | GCNNGC | 1 cut(s) | 550 |
| CciI | TCATGA | 1 cut(s) | 1344 |
| CfoI | GCGC | 1 cut(s) | 1620 |
| Cfr13I | GGNCC | 2 cut(s) | 122, 1265 |
| CseI | GACGC | 2 cut(s) | 1226, 1285 |
| Csp6I | GTAC | 2 cut(s) | 779, 1477 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 579, 614 |
| CviAII | CATG | 5 cut(s) | 271, 866, 935, 1024, 1345 |
| CviQI | GTAC | 2 cut(s) | 779, 1477 |
| DdeI | CTNAG | 1 cut(s) | 1638 |
| DpnI | GATC | 9 cut(s) | 100, 152, 423, 585, 747, 1118, 1126, 1443, 1484 |
| DpnII | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| DraI | TTTAAA | 1 cut(s) | 624 |
| EaeI | YGGCCR | 1 cut(s) | 729 |
| Eam1104I | CTCTTC | 1 cut(s) | 1098 |
| EarI | CTCTTC | 1 cut(s) | 1098 |
| Eco130I | CCWWGG | 2 cut(s) | 331, 1574 |
| Eco31I | GGTCTC | 1 cut(s) | 936 |
| Eco47I | GGWCC | 2 cut(s) | 122, 1265 |
| Eco57I | CTGAAG | 3 cut(s) | 39, 1119, 1310 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 367 |
| EcoRII | CCWGG | 3 cut(s) | 154, 362, 763 |
| EcoT14I | CCWWGG | 2 cut(s) | 331, 1574 |
| EcoT22I | ATGCAT | 2 cut(s) | 10, 1463 |
| ErhI | CCWWGG | 2 cut(s) | 331, 1574 |
| FaeI | CATG | 5 cut(s) | 274, 869, 938, 1027, 1348 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1112, 1144, 1554, 1586 |
| FaqI | GGGAC | 2 cut(s) | 149, 183 |
| FatI | CATG | 5 cut(s) | 270, 865, 934, 1023, 1344 |
| FbaI | TGATCA | 2 cut(s) | 421, 1116 |
| FblI | GTMKAC | 3 cut(s) | 484, 1299, 1596 |
| Fnu4HI | GCNGC | 4 cut(s) | 81, 84, 984, 987 |
| FokI | GGATG | 4 cut(s) | 344, 816, 939, 1029 |
| Fsp4HI | GCNGC | 4 cut(s) | 81, 84, 984, 987 |
| FspBI | CTAG | 1 cut(s) | 369 |
| GlaI | GCGC | 1 cut(s) | 1619 |
| GluI | GCNGC | 4 cut(s) | 81, 84, 984, 987 |
| HaeIII | GGCC | 1 cut(s) | 731 |
| HgaI | GACGC | 2 cut(s) | 1226, 1285 |
| HhaI | GCGC | 1 cut(s) | 1620 |
| Hin1II | CATG | 5 cut(s) | 274, 869, 938, 1027, 1348 |
| Hin6I | GCGC | 1 cut(s) | 1618 |
| HinP1I | GCGC | 1 cut(s) | 1618 |
| HincII | GTYRAC | 1 cut(s) | 1597 |
| HindII | GTYRAC | 1 cut(s) | 1597 |
| HinfI | GANTC | 8 cut(s) | 69, 242, 287, 341, 373, 1254, 1534, 1631 |
| HphI | GGTGA | 1 cut(s) | 1519 |
| Hpy166II | GTNNAC | 8 cut(s) | 39, 276, 406, 485, 662, 917, 1300, 1597 |
| Hpy188I | TCNGA | 6 cut(s) | 247, 381, 964, 1043, 1329, 1384 |
| Hpy188III | TCNNGA | 4 cut(s) | 454, 1345, 1418, 1486 |
| Hpy8I | GTNNAC | 8 cut(s) | 39, 276, 406, 485, 662, 917, 1300, 1597 |
| HpyAV | CCTTC | 3 cut(s) | 89, 1138, 1556 |
| HpyCH4III | ACNGT | 3 cut(s) | 434, 659, 874 |
| HpyCH4IV | ACGT | 3 cut(s) | 30, 172, 1176 |
| HpyCH4V | TGCA | 6 cut(s) | 8, 86, 548, 986, 1247, 1461 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 110, 1190 |
| HpyF3I | CTNAG | 1 cut(s) | 1638 |
| HpySE526I | ACGT | 3 cut(s) | 30, 172, 1176 |
| Hsp92II | CATG | 5 cut(s) | 274, 869, 938, 1027, 1348 |
| HspAI | GCGC | 1 cut(s) | 1618 |
| Ksp22I | TGATCA | 2 cut(s) | 421, 1116 |
| Kzo9I | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| LmnI | GCTCC | 3 cut(s) | 635, 763, 1578 |
| Lsp1109I | GCAGC | 4 cut(s) | 67, 70, 970, 998 |
| LweI | GCATC | 4 cut(s) | 17, 561, 794, 1152 |
| MaeI | CTAG | 1 cut(s) | 369 |
| MaeII | ACGT | 3 cut(s) | 30, 172, 1176 |
| MaeIII | GTNAC | 2 cut(s) | 565, 841 |
| MalI | GATC | 9 cut(s) | 100, 152, 423, 585, 747, 1118, 1126, 1443, 1484 |
| MbiI | CCGCTC | 1 cut(s) | 1581 |
| MboI | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| MflI | RGATCY | 3 cut(s) | 150, 1124, 1441 |
| MlsI | TGGCCA | 1 cut(s) | 731 |
| MluNI | TGGCCA | 1 cut(s) | 731 |
| MlyI | GAGTC | 3 cut(s) | 78, 1263, 1528 |
| MnlI | CCTC | 5 cut(s) | 392, 406, 512, 869, 1278 |
| Mox20I | TGGCCA | 1 cut(s) | 731 |
| Mph1103I | ATGCAT | 2 cut(s) | 10, 1463 |
| MroXI | GAANNNNTTC | 2 cut(s) | 644, 1461 |
| MscI | TGGCCA | 1 cut(s) | 731 |
| MseI | TTAA | 6 cut(s) | 437, 623, 672, 879, 1259, 1658 |
| Msp20I | TGGCCA | 1 cut(s) | 731 |
| MspR9I | CCNGG | 3 cut(s) | 156, 364, 765 |
| Mva1269I | GAATGC | 4 cut(s) | 10, 1461, 1463, 1620 |
| MvaI | CCWGG | 3 cut(s) | 156, 364, 765 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 110, 1190 |
| NdeII | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| NlaIII | CATG | 5 cut(s) | 274, 869, 938, 1027, 1348 |
| NlaIV | GGNNCC | 3 cut(s) | 1029, 1126, 1443 |
| NmuCI | GTSAC | 1 cut(s) | 841 |
| NsiI | ATGCAT | 2 cut(s) | 10, 1463 |
| NspI | RCATGY | 1 cut(s) | 274 |
| PagI | TCATGA | 1 cut(s) | 1344 |
| PaqCI | CACCTGC | 1 cut(s) | 759 |
| PciI | ACATGT | 1 cut(s) | 270 |
| PctI | GAATGC | 4 cut(s) | 10, 1461, 1463, 1620 |
| PdmI | GAANNNNTTC | 2 cut(s) | 644, 1461 |
| PfeI | GAWTC | 5 cut(s) | 242, 287, 341, 373, 1631 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 363 |
| PkrI | GCNGC | 4 cut(s) | 82, 85, 985, 988 |
| PleI | GAGTC | 3 cut(s) | 77, 1262, 1528 |
| PpsI | GAGTC | 3 cut(s) | 77, 1262, 1528 |
| PscI | ACATGT | 1 cut(s) | 270 |
| Psp1406I | AACGTT | 1 cut(s) | 1176 |
| Psp6I | CCWGG | 3 cut(s) | 154, 362, 763 |
| PspGI | CCWGG | 3 cut(s) | 154, 362, 763 |
| PspN4I | GGNNCC | 3 cut(s) | 1029, 1126, 1443 |
| PspPI | GGNCC | 2 cut(s) | 122, 1265 |
| PstI | CTGCAG | 1 cut(s) | 988 |
| PsuI | RGATCY | 3 cut(s) | 150, 1124, 1441 |
| RsaI | GTAC | 2 cut(s) | 780, 1478 |
| RsaNI | GTAC | 2 cut(s) | 779, 1477 |
| SalI | GTCGAC | 1 cut(s) | 1595 |
| SaqAI | TTAA | 6 cut(s) | 437, 623, 672, 879, 1259, 1658 |
| SatI | GCNGC | 4 cut(s) | 81, 84, 984, 987 |
| Sau3AI | GATC | 9 cut(s) | 98, 150, 421, 583, 745, 1116, 1124, 1441, 1482 |
| Sau96I | GGNCC | 2 cut(s) | 122, 1265 |
| SchI | GAGTC | 3 cut(s) | 78, 1263, 1528 |
| ScrFI | CCNGG | 3 cut(s) | 156, 364, 765 |
| SfaNI | GCATC | 4 cut(s) | 17, 561, 794, 1152 |
| SfcI | CTRYAG | 2 cut(s) | 886, 984 |
| SinI | GGWCC | 2 cut(s) | 122, 1265 |
| SmlI | CTYRAG | 1 cut(s) | 1613 |
| SmoI | CTYRAG | 1 cut(s) | 1613 |
| SsiI | CCGC | 1 cut(s) | 1581 |
| SspMI | CTAG | 1 cut(s) | 369 |
| StyD4I | CCNGG | 3 cut(s) | 154, 362, 763 |
| StyI | CCWWGG | 2 cut(s) | 331, 1574 |
| TaaI | ACNGT | 3 cut(s) | 434, 659, 874 |
| TaiI | ACGT | 3 cut(s) | 33, 175, 1179 |
| TaqI | TCGA | 6 cut(s) | 519, 1131, 1425, 1468, 1542, 1596 |
| TatI | WGTACW | 2 cut(s) | 778, 1476 |
| TfiI | GAWTC | 5 cut(s) | 242, 287, 341, 373, 1631 |
| Tru1I | TTAA | 6 cut(s) | 437, 623, 672, 879, 1259, 1658 |
| Tru9I | TTAA | 6 cut(s) | 437, 623, 672, 879, 1259, 1658 |
| TscAI | CASTG | 4 cut(s) | 82, 664, 1102, 1535 |
| TseFI | GTSAC | 1 cut(s) | 841 |
| TseI | GCWGC | 4 cut(s) | 80, 83, 983, 986 |
| Tsp45I | GTSAC | 1 cut(s) | 841 |
| TspGWI | ACGGA | 1 cut(s) | 696 |
| TspRI | CASTG | 4 cut(s) | 82, 664, 1102, 1535 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 363 |
| VpaK11BI | GGWCC | 2 cut(s) | 122, 1265 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 367 |
| XapI | RAATTY | 2 cut(s) | 996, 1651 |
| XceI | RCATGY | 1 cut(s) | 274 |
| XmiI | GTMKAC | 3 cut(s) | 484, 1299, 1596 |
| XmnI | GAANNNNTTC | 2 cut(s) | 644, 1461 |
| XspI | CTAG | 1 cut(s) | 369 |
| Zsp2I | ATGCAT | 2 cut(s) | 10, 1463 |