Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 762 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 357 |
| AccBSI | CCGCTC | 1 cut(s) | 1389 |
| AciI | CCGC | 3 cut(s) | 14, 1021, 1389 |
| AclWI | GGATC | 4 cut(s) | 187, 656, 1322, 1335 |
| AcsI | RAATTY | 5 cut(s) | 233, 578, 638, 894, 1338 |
| AcuI | CTGAAG | 2 cut(s) | 1158, 1337 |
| AfaI | GTAC | 2 cut(s) | 1244, 1364 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 357, 1290 |
| AflII | CTTAAG | 1 cut(s) | 1322 |
| AflIII | ACRYGT | 1 cut(s) | 264 |
| AgsI | TTSAA | 6 cut(s) | 176, 553, 686, 908, 1343, 1439 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 968 |
| AjnI | CCWGG | 2 cut(s) | 130, 362 |
| AluBI | AGCT | 6 cut(s) | 500, 781, 881, 944, 1124, 1523 |
| AluI | AGCT | 6 cut(s) | 500, 781, 881, 944, 1124, 1523 |
| Alw26I | GTCTC | 4 cut(s) | 157, 955, 998, 1242 |
| AlwI | GGATC | 4 cut(s) | 187, 656, 1322, 1335 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 725 |
| AoxI | GGCC | 2 cut(s) | 273, 520 |
| ApeKI | GCWGC | 3 cut(s) | 161, 881, 884 |
| ApoI | RAATTY | 5 cut(s) | 233, 578, 638, 894, 1338 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 195, 227 |
| AseI | ATTAAT | 1 cut(s) | 626 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 548 |
| AspLEI | GCGC | 2 cut(s) | 763, 1506 |
| AspS9I | GGNCC | 6 cut(s) | 77, 203, 274, 360, 1023, 1163 |
| AsuHPI | GGTGA | 3 cut(s) | 3, 146, 985 |
| AvaII | GGWCC | 5 cut(s) | 77, 203, 360, 1023, 1163 |
| AxyI | CCTNAGG | 1 cut(s) | 375 |
| BamHI | GGATCC | 1 cut(s) | 1327 |
| BanII | GRGCYC | 1 cut(s) | 1423 |
| BbsI | GAAGAC | 1 cut(s) | 879 |
| BbvI | GCAGC | 3 cut(s) | 148, 868, 896 |
| BccI | CCATC | 5 cut(s) | 359, 909, 1006, 1142, 1369 |
| BceAI | ACGGC | 2 cut(s) | 32, 288 |
| BciT130I | CCWGG | 2 cut(s) | 132, 364 |
| BclI | TGATCA | 1 cut(s) | 415 |
| BcoDI | GTCTC | 4 cut(s) | 157, 955, 998, 1242 |
| BfaI | CTAG | 2 cut(s) | 1223, 1304 |
| BfmI | CTRYAG | 5 cut(s) | 784, 882, 1105, 1192, 1211 |
| BfrI | CTTAAG | 1 cut(s) | 1322 |
| BisI | GCNGC | 3 cut(s) | 162, 882, 885 |
| BlsI | GCNGC | 3 cut(s) | 163, 883, 886 |
| Bme1390I | CCNGG | 2 cut(s) | 132, 364 |
| Bme18I | GGWCC | 5 cut(s) | 77, 203, 360, 1023, 1163 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 968 |
| BmgT120I | GGNCC | 6 cut(s) | 77, 203, 274, 360, 1023, 1163 |
| BmiI | GGNNCC | 4 cut(s) | 382, 927, 1329, 1532 |
| BmrFI | CCNGG | 2 cut(s) | 132, 364 |
| BmrI | ACTGGG | 1 cut(s) | 1426 |
| BmsI | GCATC | 3 cut(s) | 98, 465, 478 |
| BmuI | ACTGGG | 1 cut(s) | 1426 |
| BpiI | GAAGAC | 1 cut(s) | 879 |
| BpuEI | CTTGAG | 3 cut(s) | 84, 406, 1484 |
| Bsa29I | ATCGAT | 1 cut(s) | 1482 |
| BsaBI | GATNNNNATC | 2 cut(s) | 611, 1379 |
| BsaI | GGTCTC | 1 cut(s) | 1242 |
| BsaJI | CCNNGG | 5 cut(s) | 17, 130, 325, 950, 1402 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 579, 609 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 357, 1290 |
| Bse118I | RCCGGY | 1 cut(s) | 767 |
| Bse1I | ACTGG | 1 cut(s) | 1421 |
| Bse21I | CCTNAGG | 1 cut(s) | 375 |
| Bse8I | GATNNNNATC | 2 cut(s) | 611, 1379 |
| BseBI | CCWGG | 2 cut(s) | 132, 364 |
| BseCI | ATCGAT | 1 cut(s) | 1482 |
| BseDI | CCNNGG | 5 cut(s) | 17, 130, 325, 950, 1402 |
| BseGI | GGATG | 4 cut(s) | 45, 89, 920, 1153 |
| BseJI | GATNNNNATC | 2 cut(s) | 611, 1379 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 357, 1290 |
| BseMII | CTCAG | 1 cut(s) | 717 |
| BseNI | ACTGG | 1 cut(s) | 1421 |
| BseXI | GCAGC | 3 cut(s) | 148, 868, 896 |
| BshFI | GGCC | 2 cut(s) | 275, 522 |
| BshVI | ATCGAT | 1 cut(s) | 1482 |
| BsiSI | CCGG | 1 cut(s) | 768 |
| BslFI | GGGAC | 3 cut(s) | 230, 852, 1307 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 357, 1290 |
| BsmAI | GTCTC | 4 cut(s) | 157, 955, 998, 1242 |
| BsmBI | CGTCTC | 1 cut(s) | 998 |
| BsmFI | GGGAC | 3 cut(s) | 230, 852, 1307 |
| BsmI | GAATGC | 2 cut(s) | 1347, 1506 |
| BsnI | GGCC | 2 cut(s) | 275, 522 |
| Bso31I | GGTCTC | 1 cut(s) | 1242 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1423 |
| Bsp143I | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| Bsp19I | CCATGG | 1 cut(s) | 17 |
| BspACI | CCGC | 3 cut(s) | 14, 1021, 1389 |
| BspANI | GGCC | 2 cut(s) | 275, 522 |
| BspCNI | CTCAG | 1 cut(s) | 718 |
| BspDI | ATCGAT | 1 cut(s) | 1482 |
| BspLI | GGNNCC | 4 cut(s) | 382, 927, 1329, 1532 |
| BspMAI | CTGCAG | 2 cut(s) | 886, 1215 |
| BspPI | GGATC | 4 cut(s) | 187, 656, 1322, 1335 |
| BspTI | CTTAAG | 1 cut(s) | 1322 |
| BspTNI | GGTCTC | 1 cut(s) | 1242 |
| BsrBI | CCGCTC | 1 cut(s) | 1389 |
| BsrFI | RCCGGY | 1 cut(s) | 767 |
| BsrI | ACTGG | 1 cut(s) | 1421 |
| BssAI | RCCGGY | 1 cut(s) | 767 |
| BssECI | CCNNGG | 5 cut(s) | 17, 130, 325, 950, 1402 |
| BssMI | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| BssT1I | CCWWGG | 3 cut(s) | 17, 325, 950 |
| Bst2UI | CCWGG | 2 cut(s) | 132, 364 |
| Bst4CI | ACNGT | 2 cut(s) | 966, 1196 |
| Bst6I | CTCTTC | 1 cut(s) | 193 |
| BstAFI | CTTAAG | 1 cut(s) | 1322 |
| BstC8I | GCNNGC | 3 cut(s) | 765, 980, 1080 |
| BstDEI | CTNAG | 4 cut(s) | 375, 726, 1524, 1536 |
| BstDSI | CCRYGG | 1 cut(s) | 17 |
| BstF5I | GGATG | 4 cut(s) | 45, 89, 920, 1153 |
| BstHHI | GCGC | 2 cut(s) | 763, 1506 |
| BstKTI | GATC | 8 cut(s) | 182, 256, 418, 652, 664, 1117, 1330, 1371 |
| BstMAI | GTCTC | 4 cut(s) | 157, 955, 998, 1242 |
| BstMBI | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 191, 497, 1065 |
| BstNI | CCWGG | 2 cut(s) | 132, 364 |
| BstNSI | RCATGY | 2 cut(s) | 268, 767 |
| BstSCI | CCNGG | 2 cut(s) | 130, 362 |
| BstSFI | CTRYAG | 5 cut(s) | 784, 882, 1105, 1192, 1211 |
| BstV1I | GCAGC | 3 cut(s) | 148, 868, 896 |
| BstV2I | GAAGAC | 1 cut(s) | 879 |
| BstX2I | RGATCY | 1 cut(s) | 1327 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 213 |
| BstYI | RGATCY | 1 cut(s) | 1327 |
| Bsu15I | ATCGAT | 1 cut(s) | 1482 |
| Bsu36I | CCTNAGG | 1 cut(s) | 375 |
| BsuRI | GGCC | 2 cut(s) | 275, 522 |
| BsuTUI | ATCGAT | 1 cut(s) | 1482 |
| BtgI | CCRYGG | 1 cut(s) | 17 |
| BtsCI | GGATG | 4 cut(s) | 45, 89, 920, 1153 |
| BtsIMutI | CAGTG | 2 cut(s) | 723, 1414 |
| Cac8I | GCNNGC | 3 cut(s) | 765, 980, 1080 |
| CaiI | CAGNNNCTG | 1 cut(s) | 725 |
| CfoI | GCGC | 2 cut(s) | 763, 1506 |
| Cfr10I | RCCGGY | 1 cut(s) | 767 |
| Cfr13I | GGNCC | 6 cut(s) | 77, 203, 274, 360, 1023, 1163 |
| ClaI | ATCGAT | 1 cut(s) | 1482 |
| Csp6I | GTAC | 2 cut(s) | 1243, 1363 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 483, 518 |
| CviAII | CATG | 4 cut(s) | 18, 265, 699, 764 |
| CviQI | GTAC | 2 cut(s) | 1243, 1363 |
| DdeI | CTNAG | 4 cut(s) | 375, 726, 1524, 1536 |
| DpnI | GATC | 8 cut(s) | 181, 255, 417, 651, 663, 1116, 1329, 1370 |
| DpnII | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| DraI | TTTAAA | 1 cut(s) | 577 |
| DriI | GACNNNNNGTC | 1 cut(s) | 968 |
| Eam1104I | CTCTTC | 1 cut(s) | 193 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 968 |
| EarI | CTCTTC | 1 cut(s) | 193 |
| Eco130I | CCWWGG | 3 cut(s) | 17, 325, 950 |
| Eco147I | AGGCCT | 1 cut(s) | 522 |
| Eco24I | GRGCYC | 1 cut(s) | 1423 |
| Eco31I | GGTCTC | 1 cut(s) | 1242 |
| Eco47I | GGWCC | 5 cut(s) | 77, 203, 360, 1023, 1163 |
| Eco57I | CTGAAG | 2 cut(s) | 1158, 1337 |
| Eco81I | CCTNAGG | 1 cut(s) | 375 |
| EcoO109I | RGGNCCY | 1 cut(s) | 77 |
| EcoRI | GAATTC | 1 cut(s) | 1338 |
| EcoRII | CCWGG | 2 cut(s) | 130, 362 |
| EcoT14I | CCWWGG | 3 cut(s) | 17, 325, 950 |
| EcoT22I | ATGCAT | 2 cut(s) | 91, 1349 |
| EcoT38I | GRGCYC | 1 cut(s) | 1423 |
| ErhI | CCWWGG | 3 cut(s) | 17, 325, 950 |
| Esp3I | CGTCTC | 1 cut(s) | 998 |
| FaeI | CATG | 4 cut(s) | 21, 268, 702, 767 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 368, 400, 1010, 1042 |
| FaqI | GGGAC | 3 cut(s) | 230, 852, 1307 |
| FatI | CATG | 4 cut(s) | 17, 264, 698, 763 |
| FauNDI | CATATG | 1 cut(s) | 466 |
| FbaI | TGATCA | 1 cut(s) | 415 |
| Fnu4HI | GCNGC | 3 cut(s) | 162, 882, 885 |
| FokI | GGATG | 4 cut(s) | 52, 76, 927, 1160 |
| FriOI | GRGCYC | 1 cut(s) | 1423 |
| Fsp4HI | GCNGC | 3 cut(s) | 162, 882, 885 |
| FspBI | CTAG | 2 cut(s) | 1223, 1304 |
| FspI | TGCGCA | 1 cut(s) | 762 |
| GlaI | GCGC | 2 cut(s) | 762, 1505 |
| GluI | GCNGC | 3 cut(s) | 162, 882, 885 |
| HaeIII | GGCC | 2 cut(s) | 275, 522 |
| HapII | CCGG | 1 cut(s) | 768 |
| HhaI | GCGC | 2 cut(s) | 763, 1506 |
| Hin1II | CATG | 4 cut(s) | 21, 268, 702, 767 |
| Hin6I | GCGC | 2 cut(s) | 761, 1504 |
| HinP1I | GCGC | 2 cut(s) | 761, 1504 |
| HindIII | AAGCTT | 1 cut(s) | 498 |
| HinfI | GANTC | 6 cut(s) | 150, 281, 367, 863, 1152, 1464 |
| HpaII | CCGG | 1 cut(s) | 768 |
| HphI | GGTGA | 3 cut(s) | 3, 146, 985 |
| Hpy166II | GTNNAC | 5 cut(s) | 270, 400, 566, 815, 1134 |
| Hpy188I | TCNGA | 4 cut(s) | 124, 801, 862, 1403 |
| Hpy188III | TCNNGA | 7 cut(s) | 101, 448, 1089, 1176, 1223, 1372, 1384 |
| Hpy8I | GTNNAC | 5 cut(s) | 270, 400, 566, 815, 1134 |
| HpyAV | CCTTC | 4 cut(s) | 170, 304, 1000, 1036 |
| HpyCH4III | ACNGT | 2 cut(s) | 966, 1196 |
| HpyCH4V | TGCA | 9 cut(s) | 89, 251, 481, 491, 635, 884, 1082, 1213, 1347 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 191, 497, 1065 |
| HpyF3I | CTNAG | 4 cut(s) | 375, 726, 1524, 1536 |
| Hsp92II | CATG | 4 cut(s) | 21, 268, 702, 767 |
| HspAI | GCGC | 2 cut(s) | 761, 1504 |
| Ksp22I | TGATCA | 1 cut(s) | 415 |
| Kzo9I | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| LmnI | GCTCC | 3 cut(s) | 539, 941, 1536 |
| Lsp1109I | GCAGC | 3 cut(s) | 148, 868, 896 |
| LweI | GCATC | 3 cut(s) | 98, 465, 478 |
| MaeI | CTAG | 2 cut(s) | 1223, 1304 |
| MaeIII | GTNAC | 1 cut(s) | 469 |
| MalI | GATC | 8 cut(s) | 181, 255, 417, 651, 663, 1116, 1329, 1370 |
| MbiI | CCGCTC | 1 cut(s) | 1389 |
| MboI | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| MboII | GAAGA | 6 cut(s) | 210, 314, 331, 362, 884, 1343 |
| MflI | RGATCY | 1 cut(s) | 1327 |
| MhlI | GDGCHC | 1 cut(s) | 1423 |
| MlyI | GAGTC | 1 cut(s) | 159 |
| MmeI | TCCRAC | 2 cut(s) | 117, 285 |
| MnlI | CCTC | 8 cut(s) | 108, 194, 287, 512, 533, 1176, 1199, 1397 |
| Mph1103I | ATGCAT | 2 cut(s) | 91, 1349 |
| MroXI | GAANNNNTTC | 1 cut(s) | 548 |
| MseI | TTAA | 6 cut(s) | 68, 576, 626, 777, 1233, 1323 |
| MslI | CAYNNNNRTG | 4 cut(s) | 16, 486, 697, 768 |
| MspCI | CTTAAG | 1 cut(s) | 1322 |
| MspI | CCGG | 1 cut(s) | 768 |
| MspR9I | CCNGG | 2 cut(s) | 132, 364 |
| Mva1269I | GAATGC | 2 cut(s) | 1347, 1506 |
| MvaI | CCWGG | 2 cut(s) | 132, 364 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 191, 497, 1065 |
| NcoI | CCATGG | 1 cut(s) | 17 |
| NdeI | CATATG | 1 cut(s) | 466 |
| NdeII | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| NlaIII | CATG | 4 cut(s) | 21, 268, 702, 767 |
| NlaIV | GGNNCC | 4 cut(s) | 382, 927, 1329, 1532 |
| NmuCI | GTSAC | 1 cut(s) | 469 |
| NsbI | TGCGCA | 1 cut(s) | 762 |
| NsiI | ATGCAT | 2 cut(s) | 91, 1349 |
| NspI | RCATGY | 2 cut(s) | 268, 767 |
| PaeI | GCATGC | 1 cut(s) | 767 |
| PceI | AGGCCT | 1 cut(s) | 522 |
| PciI | ACATGT | 1 cut(s) | 264 |
| PctI | GAATGC | 2 cut(s) | 1347, 1506 |
| PdmI | GAANNNNTTC | 1 cut(s) | 548 |
| PfeI | GAWTC | 5 cut(s) | 281, 367, 863, 1152, 1464 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 357 |
| PkrI | GCNGC | 3 cut(s) | 163, 883, 886 |
| PleI | GAGTC | 1 cut(s) | 158 |
| PpsI | GAGTC | 1 cut(s) | 158 |
| PpuMI | RGGWCCY | 1 cut(s) | 77 |
| PscI | ACATGT | 1 cut(s) | 264 |
| PshBI | ATTAAT | 1 cut(s) | 626 |
| Psp5II | RGGWCCY | 1 cut(s) | 77 |
| Psp6I | CCWGG | 2 cut(s) | 130, 362 |
| PspGI | CCWGG | 2 cut(s) | 130, 362 |
| PspN4I | GGNNCC | 4 cut(s) | 382, 927, 1329, 1532 |
| PspPI | GGNCC | 6 cut(s) | 77, 203, 274, 360, 1023, 1163 |
| PspPPI | RGGWCCY | 1 cut(s) | 77 |
| PstI | CTGCAG | 2 cut(s) | 886, 1215 |
| PstNI | CAGNNNCTG | 1 cut(s) | 725 |
| PsuI | RGATCY | 1 cut(s) | 1327 |
| RsaI | GTAC | 2 cut(s) | 1244, 1364 |
| RsaNI | GTAC | 2 cut(s) | 1243, 1363 |
| RseI | CAYNNNNRTG | 4 cut(s) | 16, 486, 697, 768 |
| SaqAI | TTAA | 6 cut(s) | 68, 576, 626, 777, 1233, 1323 |
| SatI | GCNGC | 3 cut(s) | 162, 882, 885 |
| Sau3AI | GATC | 8 cut(s) | 179, 253, 415, 649, 661, 1114, 1327, 1368 |
| Sau96I | GGNCC | 6 cut(s) | 77, 203, 274, 360, 1023, 1163 |
| SchI | GAGTC | 1 cut(s) | 159 |
| ScrFI | CCNGG | 2 cut(s) | 132, 364 |
| SduI | GDGCHC | 1 cut(s) | 1423 |
| SfaNI | GCATC | 3 cut(s) | 98, 465, 478 |
| SfcI | CTRYAG | 5 cut(s) | 784, 882, 1105, 1192, 1211 |
| SinI | GGWCC | 5 cut(s) | 77, 203, 360, 1023, 1163 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 16, 486, 697, 768 |
| SmlI | CTYRAG | 4 cut(s) | 99, 385, 1322, 1499 |
| SmoI | CTYRAG | 4 cut(s) | 99, 385, 1322, 1499 |
| SphI | GCATGC | 1 cut(s) | 767 |
| SseBI | AGGCCT | 1 cut(s) | 522 |
| SsiI | CCGC | 3 cut(s) | 14, 1021, 1389 |
| SspMI | CTAG | 2 cut(s) | 1223, 1304 |
| StuI | AGGCCT | 1 cut(s) | 522 |
| StyD4I | CCNGG | 2 cut(s) | 130, 362 |
| StyI | CCWWGG | 3 cut(s) | 17, 325, 950 |
| TaaI | ACNGT | 2 cut(s) | 966, 1196 |
| TaqI | TCGA | 3 cut(s) | 279, 1029, 1482 |
| TatI | WGTACW | 1 cut(s) | 1242 |
| TfiI | GAWTC | 5 cut(s) | 281, 367, 863, 1152, 1464 |
| Tru1I | TTAA | 6 cut(s) | 68, 576, 626, 777, 1233, 1323 |
| Tru9I | TTAA | 6 cut(s) | 68, 576, 626, 777, 1233, 1323 |
| TscAI | CASTG | 2 cut(s) | 730, 1421 |
| TseFI | GTSAC | 1 cut(s) | 469 |
| TseI | GCWGC | 3 cut(s) | 161, 881, 884 |
| Tsp45I | GTSAC | 1 cut(s) | 469 |
| TspDTI | ATGAA | 4 cut(s) | 273, 453, 1219, 1329 |
| TspRI | CASTG | 2 cut(s) | 730, 1421 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 357 |
| Vha464I | CTTAAG | 1 cut(s) | 1322 |
| VpaK11BI | GGWCC | 5 cut(s) | 77, 203, 360, 1023, 1163 |
| VspI | ATTAAT | 1 cut(s) | 626 |
| XapI | RAATTY | 5 cut(s) | 233, 578, 638, 894, 1338 |
| XbaI | TCTAGA | 1 cut(s) | 1222 |
| XceI | RCATGY | 2 cut(s) | 268, 767 |
| XmnI | GAANNNNTTC | 1 cut(s) | 548 |
| XspI | CTAG | 2 cut(s) | 1223, 1304 |
| Zsp2I | ATGCAT | 2 cut(s) | 91, 1349 |