Rw3G018030

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000392
Physical Location & Seq
Forward (+)
19881524 .. 19884029
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018030.1

Sequence Viewer

Length: 1446 bp
ATGAAATGCACCTATGAATGGTTATACCATGTGCAGATTGAAGGATCAACAAATAAAGCAGGACGAGGACCAAGTGTTTGGGACGAGTTTGTTAAGACATTCCCAGAACGCATTGCAGACCATTCTACCATGTCTGCGGCAATCGACTCATACAAGCGATACAAGGAAGATGTGAAGGCAGTGAAAGCCCTTGGAGTTGATTCTTATAGATTTTCCATCTCTTGGACCAGGATTCTTCCCAAGGGAACCTTGAGTGGAGGAATAAACCAAGAGGGTATTGATCACTACAACAACTTGATTGATGAACTAATCAAGAATGGCATCACACCCTATGTAACCATTTTACATTTTGATCCACCACAAGCCTTGACAGTCAAGTATGGAGGCTTTCTCGATCGCTCCTTTGTGAATGACTTTAAGGATTACAGTGAACTTTGCTTTAAGATCTATGGAGATAGAGTCAAAAATTGGATTACAATCAACGAGCCTTTTATTATAGCCAAAATGGGCTATGATCTTGGGGTTGCTCCACCAGGCAGGTGTTCCATACCAGGTGGTCATTTCCATTATTGCACAGCTGGAAATTCATCTACTGAACCTTACATTGTGGTTCATAACCTTCTCCTTGCCCATGCCACTGTTGTTAAGCTCTATAGAGAAAAGTTTCAGACAAAACAAGGTGGACAAATTGGATGGAGTCTTGTAGGTTCATATGTTGAGCCTTATTCAAATTCATCTAAGGACAAAGCTGCAGCAAAAAGAATTTTGGACTTTGAACTTGGATGGTTCATGGAACCAATAGTATATGGACATTATCCAAAGAGTATGAGGCGCTTGGTCAGGAAAAGGCTACCCCGTTTCACCAAAGAAGAGAAGAAATTGATTAAAGGATCCTTTGATTTCATTGGCATCAACTATTATACCTCGAGATATGGTAGAAACATTCCAGCACGTCTAGATATGCCAAACTTCTATCGCTATGATCCTCTAGCGTCATCAGAGCCCAAGAATGTAAATGGAGTCCCAATTGGCCCTCAGGCTGATGGTAGCATGTTCATCTTGTCTTATCCACAAGGTCTCAAGAAACTTTTGAAGTTCATGAAGCGAAAATACCAGAGCCCTAAGATCTACATTTCCGAAAATGGAATTACAGAGGCAAAGGACGATAAGCGTGGACTTGATGTAGCACTGAAGGATCCACATAGAATTGAATGTATTCTTCGGCATTTGTACATGATCAAGCAGGCCATAAAGAAGGGTGTGAACGTCAAAGGATATTTCCACTGGGCTCTATTTGACGATTTTGAGTGGGGGGAAGGCTATATCCCAAGGTACGGGCTTTATTATGTCGACTACAATGACAATCTCAAGCGCATTCCTAAAGAATCTGCAAAGTGGCTCCCTAAATTCCTAAAAGGCGAAGATGCGATACATGTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

481

Amino Acids

55.33

Weight (kDa)

9.35

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 12 - 472 6.6e-143 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25630 AT2G44450 AT2G44450 AT3G60130 AT3G60130 AT3G60130 AT5G42260 AT5G44640
fragaria_vesca FvH4_5g07160 FvH4_5g07180 FvH4_5g07811 FvH4_5g07831 FvH4_5g07831 FvH4_5g07832 FvH4_6g19950 FvH4_6g19950 FvH4_6g19950
malus_domestica MD01G1120900.v1.1 MD06G1144600.v1.1 MD06G1144800.v1.1 MD06G1145700.v1.1 MD06G1146100.v1.1 MD06G1147000.v1.1 MD08G1072600.v1.1 MD08G1142300.v1.1 MD08G1191100.v1.1 MD14G1159400.v1.1 MD14G1160300.v1.1 MD14G1160600.v1.1 MD14G1160700.v1.1 MD14G1161400.v1.1
prunus_persica Prupe.1G525400_v2.0.a1 Prupe.1G583900_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G153200_v2.0.a1 Prupe.5G156500_v2.0.a1
pyrus_communis pycom06g13390 pycom06g13520 pycom06g13560 pycom06g13640 pycom08g12030 pycom14g13310 pycom14g13430
rosa_chinensis RchiOBHm_Chr3g0475401 RchiOBHm_Chr7g0190551 RchiOBHm_Chr7g0190631 RchiOBHm_Chr7g0190641 RchiOBHm_Chr7g0191241 RchiOBHm_Chr7g0191251 RchiOBHm_Chr7g0191261 RchiOBHm_Chr7g0202591
rosa_laevigata RLG00000004507 RLG00000004508 RLG00000004516 RLG00000023846 RLG00000035079
rosa_multiflora Rmu_co8005672.1_g000001 Rmu_co8018538.1_g000001 Rmu_sc0000526.1_g000002 Rmu_sc0001786.1_g000011 Rmu_sc0006709.1_g000007 Rmu_sc0006709.1_g000008 Rmu_sc0016637.1_g000004
rosa_roxburghii Rroxscaffold_1G00018560 Rroxscaffold_1G00024050 Rroxscaffold_3G00264440 Rroxscaffold_3G00264450 Rroxscaffold_4G00306640 Rroxscaffold_6G00406340
rosa_rugosa Rorug03G0295600 Rorug05G0298500 Rorug06G0072900 Rorug06G0505100 Rorug06G0505800 Rorug06G0505900 Rorug06G0506000
rosa_samantha Rh1CG344200 Rh2AG107900 Rh3AG198700 Rh3CG223000 Rh5AG369500 Rh5BG469700 Rh5BG469800 Rh5DG483000 Rh7AG111700 Rh7AG112400 Rh7AG112500 Rh7AG112600 Rh7CG116400 Rh7CG117400 Rh7CG117500 Rh7CG117600 Rh7CG122100 Rh7CG122200 Rh7DG115600 Rh7DG116400 Rh7DG116500
rosa_wichuraiana Rw3G018030 Rw7G009740 Rw7G009810 Rw7G009820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 528
AccB7I CCANNNNNTGG 1 cut(s) 222
AccI GTMKAC 1 cut(s) 1350
AciI CCGC 1 cut(s) 137
AclWI GGATC 7 cut(s) 52, 347, 885, 898, 977, 1190, 1203
AcsI RAATTY 4 cut(s) 583, 730, 762, 1406
AcuI CTGAAG 1 cut(s) 1211
AfaI GTAC 2 cut(s) 1232, 1334
AfiI CCNNNNNNNGG 3 cut(s) 18, 222, 1334
AflIII ACRYGT 1 cut(s) 1432
AgsI TTSAA 5 cut(s) 41, 729, 776, 1093, 1211
AjiI CACGTC 1 cut(s) 953
AjnI CCWGG 3 cut(s) 227, 532, 550
AluBI AGCT 4 cut(s) 578, 649, 749, 1442
AluI AGCT 4 cut(s) 578, 649, 749, 1442
Alw26I GTCTC 1 cut(s) 1082
AlwI GGATC 7 cut(s) 52, 347, 885, 898, 977, 1190, 1203
Ama87I CYCGRG 1 cut(s) 925
AoxI GGCC 2 cut(s) 1030, 1245
ApeKI GCWGC 2 cut(s) 749, 752
ApoI RAATTY 4 cut(s) 583, 730, 762, 1406
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
Asp700I GAANNNNTTC 2 cut(s) 663, 1215
AspLEI GCGC 2 cut(s) 834, 1374
AspS9I GGNCC 3 cut(s) 68, 225, 1031
AsuHPI GGTGA 1 cut(s) 853
AvaI CYCGRG 1 cut(s) 925
AvaII GGWCC 2 cut(s) 68, 225
AxyI CCTNAGG 1 cut(s) 1035
BamHI GGATCC 2 cut(s) 890, 1195
BanII GRGCYC 3 cut(s) 1005, 1121, 1291
BarI GAAGNNNNNNTAC 4 cut(s) 1094, 1126, 1413, 1445
BbvI GCAGC 2 cut(s) 736, 764
BccI CCATC 4 cut(s) 224, 687, 777, 1037
BciT130I CCWGG 3 cut(s) 229, 534, 552
BclI TGATCA 2 cut(s) 280, 1236
BcoDI GTCTC 1 cut(s) 1082
BfaI CTAG 2 cut(s) 956, 989
BfmI CTRYAG 2 cut(s) 652, 750
BfoI RGCGCY 1 cut(s) 835
BfuAI ACCTGC 1 cut(s) 528
BglII AGATCT 2 cut(s) 444, 1125
BisI GCNGC 3 cut(s) 138, 750, 753
BlsI GCNGC 3 cut(s) 139, 751, 754
Bme1390I CCNGG 3 cut(s) 229, 534, 552
Bme18I GGWCC 2 cut(s) 68, 225
BmeT110I CYCGRG 1 cut(s) 925
BmgBI CACGTC 1 cut(s) 953
BmgT120I GGNCC 3 cut(s) 68, 225, 1031
BmiI GGNNCC 5 cut(s) 247, 795, 892, 1197, 1400
BmrFI CCNGG 3 cut(s) 229, 534, 552
BmrI ACTGGG 1 cut(s) 1294
BmsI GCATC 3 cut(s) 330, 918, 1414
BmuI ACTGGG 1 cut(s) 1294
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpuEI CTTGAG 3 cut(s) 271, 1064, 1352
BsaBI GATNNNNATC 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 1082
BsaJI CCNNGG 3 cut(s) 190, 240, 1328
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 222, 1334
Bse1I ACTGG 1 cut(s) 1289
Bse21I CCTNAGG 1 cut(s) 1035
Bse3DI GCAATG 1 cut(s) 111
Bse8I GATNNNNATC 1 cut(s) 476
BseBI CCWGG 3 cut(s) 229, 534, 552
BseDI CCNNGG 3 cut(s) 190, 240, 1328
BseGI GGATG 2 cut(s) 698, 788
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 3 cut(s) 18, 222, 1334
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 1049
BseNI ACTGG 1 cut(s) 1289
BseXI GCAGC 2 cut(s) 736, 764
BsgI GTGCAG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 1032, 1247
BsiEI CGRYCG 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 925
BslFI GGGAC 2 cut(s) 95, 1007
BslI CCNNNNNNNGG 3 cut(s) 18, 222, 1334
BsmAI GTCTC 1 cut(s) 1082
BsmFI GGGAC 2 cut(s) 95, 1007
BsmI GAATGC 1 cut(s) 1374
BsnI GGCC 2 cut(s) 1032, 1247
Bso31I GGTCTC 1 cut(s) 1082
BsoBI CYCGRG 1 cut(s) 925
Bsp1286I GDGCHC 3 cut(s) 1005, 1121, 1291
Bsp1407I TGTACA 1 cut(s) 1230
BspACI CCGC 1 cut(s) 137
BspANI GGCC 2 cut(s) 1032, 1247
BspCNI CTCAG 1 cut(s) 1048
BspHI TCATGA 1 cut(s) 1098
BspLI GGNNCC 5 cut(s) 247, 795, 892, 1197, 1400
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 528
BspPI GGATC 7 cut(s) 52, 347, 885, 898, 977, 1190, 1203
BspTNI GGTCTC 1 cut(s) 1082
BsrDI GCAATG 1 cut(s) 111
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 1 cut(s) 1289
BssECI CCNNGG 3 cut(s) 190, 240, 1328
BssT1I CCWWGG 3 cut(s) 190, 240, 1328
Bst2UI CCWGG 3 cut(s) 229, 534, 552
Bst4CI ACNGT 3 cut(s) 373, 428, 640
Bst6I CTCTTC 1 cut(s) 864
BstAUI TGTACA 1 cut(s) 1230
BstC8I GCNNGC 1 cut(s) 1245
BstDEI CTNAG 4 cut(s) 738, 1035, 1122, 1443
BstF5I GGATG 2 cut(s) 698, 788
BstH2I RGCGCY 1 cut(s) 835
BstHHI GCGC 2 cut(s) 834, 1374
BstMAI GTCTC 1 cut(s) 1082
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 3 cut(s) 229, 534, 552
BstNSI RCATGY 2 cut(s) 1054, 1436
BstSCI CCNGG 3 cut(s) 227, 532, 550
BstSFI CTRYAG 2 cut(s) 652, 750
BstV1I GCAGC 2 cut(s) 736, 764
BstX2I RGATCY 4 cut(s) 444, 890, 1125, 1195
BstXI CCANNNNNNTGG 1 cut(s) 78
BstYI RGATCY 4 cut(s) 444, 890, 1125, 1195
Bsu36I CCTNAGG 1 cut(s) 1035
BsuRI GGCC 2 cut(s) 1032, 1247
BtrI CACGTC 1 cut(s) 953
BtsCI GGATG 2 cut(s) 698, 788
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 5 cut(s) 186, 433, 636, 1187, 1282
BveI ACCTGC 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 1245
CciI TCATGA 1 cut(s) 1098
CfoI GCGC 2 cut(s) 834, 1374
Cfr13I GGNCC 3 cut(s) 68, 225, 1031
CseI GACGC 1 cut(s) 981
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 2 cut(s) 1231, 1333
CspCI CAANNNNNGTGG 2 cut(s) 348, 383
CviAII CATG 8 cut(s) 29, 130, 632, 790, 1051, 1099, 1234, 1433
CviQI GTAC 2 cut(s) 1231, 1333
DdeI CTNAG 4 cut(s) 738, 1035, 1122, 1443
Eam1104I CTCTTC 1 cut(s) 864
EarI CTCTTC 1 cut(s) 864
Eco130I CCWWGG 3 cut(s) 190, 240, 1328
Eco24I GRGCYC 3 cut(s) 1005, 1121, 1291
Eco31I GGTCTC 1 cut(s) 1082
Eco47I GGWCC 2 cut(s) 68, 225
Eco57I CTGAAG 1 cut(s) 1211
Eco81I CCTNAGG 1 cut(s) 1035
Eco88I CYCGRG 1 cut(s) 925
EcoRII CCWGG 3 cut(s) 227, 532, 550
EcoT14I CCWWGG 3 cut(s) 190, 240, 1328
EcoT38I GRGCYC 3 cut(s) 1005, 1121, 1291
ErhI CCWWGG 3 cut(s) 190, 240, 1328
FaeI CATG 8 cut(s) 32, 133, 635, 793, 1054, 1102, 1237, 1436
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 2 cut(s) 95, 1007
FatI CATG 8 cut(s) 28, 129, 631, 789, 1050, 1098, 1233, 1432
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 2 cut(s) 280, 1236
FblI GTMKAC 1 cut(s) 1350
Fnu4HI GCNGC 3 cut(s) 138, 750, 753
FokI GGATG 2 cut(s) 705, 795
FriOI GRGCYC 3 cut(s) 1005, 1121, 1291
Fsp4HI GCNGC 3 cut(s) 138, 750, 753
FspBI CTAG 2 cut(s) 956, 989
GlaI GCGC 2 cut(s) 833, 1373
GluI GCNGC 3 cut(s) 138, 750, 753
HaeII RGCGCY 1 cut(s) 835
HaeIII GGCC 2 cut(s) 1032, 1247
HgaI GACGC 1 cut(s) 981
HhaI GCGC 2 cut(s) 834, 1374
Hin1II CATG 8 cut(s) 32, 133, 635, 793, 1054, 1102, 1237, 1436
Hin6I GCGC 2 cut(s) 832, 1372
HinP1I GCGC 2 cut(s) 832, 1372
HincII GTYRAC 1 cut(s) 1351
HindII GTYRAC 1 cut(s) 1351
HindIII AAGCTT 1 cut(s) 1440
HinfI GANTC 7 cut(s) 146, 200, 232, 459, 697, 1020, 1385
HphI GGTGA 1 cut(s) 853
Hpy166II GTNNAC 5 cut(s) 431, 683, 1175, 1264, 1351
Hpy188I TCNGA 3 cut(s) 669, 1000, 1138
Hpy188III TCNNGA 7 cut(s) 313, 392, 841, 927, 956, 1081, 1099
Hpy8I GTNNAC 5 cut(s) 431, 683, 1175, 1264, 1351
HpyAV CCTTC 6 cut(s) 35, 169, 629, 1186, 1249, 1310
HpyCH4III ACNGT 3 cut(s) 373, 428, 640
HpyCH4IV ACGT 2 cut(s) 952, 1266
HpyCH4V TGCA 6 cut(s) 9, 34, 116, 573, 752, 1391
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 4 cut(s) 738, 1035, 1122, 1443
HpySE526I ACGT 2 cut(s) 952, 1266
Hsp92II CATG 8 cut(s) 32, 133, 635, 793, 1054, 1102, 1237, 1436
HspAI GCGC 2 cut(s) 832, 1372
Ksp22I TGATCA 2 cut(s) 280, 1236
LmnI GCTCC 3 cut(s) 404, 532, 1404
Lsp1109I GCAGC 2 cut(s) 736, 764
LweI GCATC 3 cut(s) 330, 918, 1414
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 2 cut(s) 956, 989
MaeII ACGT 2 cut(s) 952, 1266
MaeIII GTNAC 1 cut(s) 334
MboII GAAGA 6 cut(s) 179, 227, 881, 886, 1211, 1433
MfeI CAATTG 1 cut(s) 1026
MflI RGATCY 4 cut(s) 444, 890, 1125, 1195
MhlI GDGCHC 3 cut(s) 1005, 1121, 1291
MlyI GAGTC 4 cut(s) 140, 468, 706, 1029
MnlI CCTC 9 cut(s) 59, 251, 265, 377, 822, 934, 996, 1044, 1147
MroXI GAANNNNTTC 2 cut(s) 663, 1215
MseI TTAA 5 cut(s) 93, 417, 441, 645, 885
MspA1I CMGCKG 1 cut(s) 578
MspR9I CCNGG 3 cut(s) 229, 534, 552
MunI CAATTG 1 cut(s) 1026
Mva1269I GAATGC 1 cut(s) 1374
MvaI CCWGG 3 cut(s) 229, 534, 552
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeI CATATG 1 cut(s) 712
NlaIII CATG 8 cut(s) 32, 133, 635, 793, 1054, 1102, 1237, 1436
NlaIV GGNNCC 5 cut(s) 247, 795, 892, 1197, 1400
NspI RCATGY 2 cut(s) 1054, 1436
PaeR7I CTCGAG 1 cut(s) 925
PagI TCATGA 1 cut(s) 1098
PaqCI CACCTGC 1 cut(s) 528
PciI ACATGT 1 cut(s) 1432
PctI GAATGC 1 cut(s) 1374
PdmI GAANNNNTTC 2 cut(s) 663, 1215
PfeI GAWTC 3 cut(s) 200, 232, 1385
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 3 cut(s) 139, 751, 754
Ple19I CGATCG 1 cut(s) 397
PleI GAGTC 4 cut(s) 140, 467, 705, 1028
PpsI GAGTC 4 cut(s) 140, 467, 705, 1028
PscI ACATGT 1 cut(s) 1432
Psp6I CCWGG 3 cut(s) 227, 532, 550
PspGI CCWGG 3 cut(s) 227, 532, 550
PspN4I GGNNCC 5 cut(s) 247, 795, 892, 1197, 1400
PspPI GGNCC 3 cut(s) 68, 225, 1031
PstI CTGCAG 1 cut(s) 754
PsuI RGATCY 4 cut(s) 444, 890, 1125, 1195
PvuI CGATCG 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 2 cut(s) 1232, 1334
RsaNI GTAC 2 cut(s) 1231, 1333
SalI GTCGAC 1 cut(s) 1349
SaqAI TTAA 5 cut(s) 93, 417, 441, 645, 885
SatI GCNGC 3 cut(s) 138, 750, 753
Sau96I GGNCC 3 cut(s) 68, 225, 1031
SchI GAGTC 4 cut(s) 140, 468, 706, 1029
ScrFI CCNGG 3 cut(s) 229, 534, 552
SduI GDGCHC 3 cut(s) 1005, 1121, 1291
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 3 cut(s) 330, 918, 1414
SfcI CTRYAG 2 cut(s) 652, 750
Sfr274I CTCGAG 1 cut(s) 925
SinI GGWCC 2 cut(s) 68, 225
SlaI CTCGAG 1 cut(s) 925
SmlI CTYRAG 4 cut(s) 250, 925, 1079, 1367
SmoI CTYRAG 4 cut(s) 250, 925, 1079, 1367
SsiI CCGC 1 cut(s) 137
SspMI CTAG 2 cut(s) 956, 989
StyD4I CCNGG 3 cut(s) 227, 532, 550
StyI CCWWGG 3 cut(s) 190, 240, 1328
TaaI ACNGT 3 cut(s) 373, 428, 640
TaiI ACGT 2 cut(s) 955, 1269
TaqI TCGA 4 cut(s) 144, 393, 926, 1350
TatI WGTACW 1 cut(s) 1230
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 3 cut(s) 200, 232, 1385
Tru1I TTAA 5 cut(s) 93, 417, 441, 645, 885
Tru9I TTAA 5 cut(s) 93, 417, 441, 645, 885
TscAI CASTG 5 cut(s) 186, 433, 643, 1194, 1289
TseI GCWGC 2 cut(s) 749, 752
TspRI CASTG 5 cut(s) 186, 433, 643, 1194, 1289
Van91I CCANNNNNTGG 1 cut(s) 222
VpaK11BI GGWCC 2 cut(s) 68, 225
XapI RAATTY 4 cut(s) 583, 730, 762, 1406
XbaI TCTAGA 1 cut(s) 955
XceI RCATGY 2 cut(s) 1054, 1436
XhoI CTCGAG 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 1350
XmnI GAANNNNTTC 2 cut(s) 663, 1215
XspI CTAG 2 cut(s) 956, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.