Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 38 |
| Acc36I | ACCTGC | 1 cut(s) | 578 |
| AccB1I | GGYRCC | 1 cut(s) | 695 |
| AccBSI | CCGCTC | 2 cut(s) | 77, 667 |
| AciI | CCGC | 7 cut(s) | 5, 62, 77, 142, 604, 665, 706 |
| AclWI | GGATC | 5 cut(s) | 212, 225, 688, 738, 858 |
| AcoI | YGGCCR | 2 cut(s) | 78, 860 |
| AcsI | RAATTY | 1 cut(s) | 814 |
| AcuI | CTGAAG | 1 cut(s) | 187 |
| AcyI | GRCGYC | 1 cut(s) | 35 |
| AfaI | GTAC | 6 cut(s) | 41, 322, 445, 472, 545, 659 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 227, 515, 894 |
| AhlI | ACTAGT | 1 cut(s) | 394 |
| AjnI | CCWGG | 1 cut(s) | 48 |
| AluBI | AGCT | 4 cut(s) | 139, 160, 198, 773 |
| AluI | AGCT | 4 cut(s) | 139, 160, 198, 773 |
| Alw21I | GWGCWC | 1 cut(s) | 141 |
| AlwI | GGATC | 5 cut(s) | 212, 225, 688, 738, 858 |
| AoxI | GGCC | 3 cut(s) | 28, 78, 860 |
| ApeKI | GCWGC | 1 cut(s) | 773 |
| ApoI | RAATTY | 1 cut(s) | 814 |
| AspA2I | CCTAGG | 1 cut(s) | 439 |
| AsuHPI | GGTGA | 2 cut(s) | 308, 428 |
| AsuII | TTCGAA | 1 cut(s) | 874 |
| AvrII | CCTAGG | 1 cut(s) | 439 |
| BalI | TGGCCA | 1 cut(s) | 862 |
| BamHI | GGATCC | 1 cut(s) | 217 |
| BanI | GGYRCC | 1 cut(s) | 695 |
| BanII | GRGCYC | 1 cut(s) | 141 |
| BbsI | GAAGAC | 1 cut(s) | 157 |
| Bbv12I | GWGCWC | 1 cut(s) | 141 |
| BbvI | GCAGC | 1 cut(s) | 760 |
| BccI | CCATC | 3 cut(s) | 229, 707, 873 |
| BciT130I | CCWGG | 1 cut(s) | 50 |
| BcuI | ACTAGT | 1 cut(s) | 394 |
| BfaI | CTAG | 4 cut(s) | 395, 440, 926, 955 |
| BfmI | CTRYAG | 1 cut(s) | 253 |
| BfuAI | ACCTGC | 1 cut(s) | 578 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 77, 868 |
| BisI | GCNGC | 3 cut(s) | 6, 78, 774 |
| BlnI | CCTAGG | 1 cut(s) | 439 |
| BlsI | GCNGC | 3 cut(s) | 7, 79, 775 |
| Bme1390I | CCNGG | 1 cut(s) | 50 |
| BmiI | GGNNCC | 4 cut(s) | 100, 219, 697, 884 |
| BmrFI | CCNGG | 1 cut(s) | 50 |
| BmrI | ACTGGG | 1 cut(s) | 882 |
| BmsI | GCATC | 2 cut(s) | 610, 938 |
| BmuI | ACTGGG | 1 cut(s) | 882 |
| BoxI | GACNNNNGTC | 1 cut(s) | 62 |
| BpiI | GAAGAC | 1 cut(s) | 157 |
| Bpu14I | TTCGAA | 1 cut(s) | 874 |
| BsaAI | YACGTR | 1 cut(s) | 543 |
| BsaHI | GRCGYC | 1 cut(s) | 35 |
| BsaJI | CCNNGG | 1 cut(s) | 439 |
| BsaWI | WCCGGW | 1 cut(s) | 727 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 350, 380 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 227, 515, 894 |
| Bse1I | ACTGG | 1 cut(s) | 888 |
| Bse3DI | GCAATG | 1 cut(s) | 369 |
| BseBI | CCWGG | 1 cut(s) | 50 |
| BseDI | CCNNGG | 1 cut(s) | 439 |
| BseGI | GGATG | 2 cut(s) | 221, 669 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 227, 515, 894 |
| BseMI | GCAATG | 1 cut(s) | 369 |
| BseMII | CTCAG | 2 cut(s) | 765, 834 |
| BseNI | ACTGG | 1 cut(s) | 888 |
| BseXI | GCAGC | 1 cut(s) | 760 |
| BsgI | GTGCAG | 1 cut(s) | 759 |
| Bsh1285I | CGRYCG | 2 cut(s) | 46, 604 |
| BshFI | GGCC | 3 cut(s) | 30, 80, 862 |
| BshNI | GGYRCC | 1 cut(s) | 695 |
| BsiEI | CGRYCG | 2 cut(s) | 46, 604 |
| BsiHKAI | GWGCWC | 1 cut(s) | 141 |
| BsiSI | CCGG | 4 cut(s) | 24, 72, 102, 728 |
| BsiWI | CGTACG | 1 cut(s) | 39 |
| BslFI | GGGAC | 2 cut(s) | 400, 781 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 227, 515, 894 |
| BsmFI | GGGAC | 2 cut(s) | 400, 781 |
| BsmI | GAATGC | 1 cut(s) | 708 |
| BsnI | GGCC | 3 cut(s) | 30, 80, 862 |
| Bsp119I | TTCGAA | 1 cut(s) | 874 |
| Bsp1286I | GDGCHC | 1 cut(s) | 141 |
| Bsp143I | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| BspACI | CCGC | 7 cut(s) | 5, 62, 77, 142, 604, 665, 706 |
| BspANI | GGCC | 3 cut(s) | 30, 80, 862 |
| BspCNI | CTCAG | 2 cut(s) | 764, 833 |
| BspHI | TCATGA | 1 cut(s) | 712 |
| BspLI | GGNNCC | 4 cut(s) | 100, 219, 697, 884 |
| BspMI | ACCTGC | 1 cut(s) | 578 |
| BspPI | GGATC | 5 cut(s) | 212, 225, 688, 738, 858 |
| BspT104I | TTCGAA | 1 cut(s) | 874 |
| BspT107I | GGYRCC | 1 cut(s) | 695 |
| BsrBI | CCGCTC | 2 cut(s) | 77, 667 |
| BsrDI | GCAATG | 1 cut(s) | 369 |
| BsrI | ACTGG | 1 cut(s) | 888 |
| BssECI | CCNNGG | 1 cut(s) | 439 |
| BssMI | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| BssNI | GRCGYC | 1 cut(s) | 35 |
| BssT1I | CCWWGG | 1 cut(s) | 439 |
| Bst2UI | CCWGG | 1 cut(s) | 50 |
| Bst4CI | ACNGT | 3 cut(s) | 320, 325, 406 |
| Bst6I | CTCTTC | 3 cut(s) | 134, 318, 909 |
| BstACI | GRCGYC | 1 cut(s) | 35 |
| BstBAI | YACGTR | 1 cut(s) | 543 |
| BstBI | TTCGAA | 1 cut(s) | 874 |
| BstC8I | GCNNGC | 2 cut(s) | 82, 563 |
| BstDEI | CTNAG | 3 cut(s) | 751, 820, 899 |
| BstF5I | GGATG | 2 cut(s) | 221, 669 |
| BstKTI | GATC | 7 cut(s) | 46, 220, 416, 683, 733, 853, 949 |
| BstMBI | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| BstMCI | CGRYCG | 2 cut(s) | 46, 604 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 68, 77, 204, 466, 868 |
| BstNI | CCWGG | 1 cut(s) | 50 |
| BstNSI | RCATGY | 2 cut(s) | 190, 565 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 62 |
| BstSCI | CCNGG | 1 cut(s) | 48 |
| BstSFI | CTRYAG | 1 cut(s) | 253 |
| BstSNI | TACGTA | 1 cut(s) | 543 |
| BstV1I | GCAGC | 1 cut(s) | 760 |
| BstV2I | GAAGAC | 1 cut(s) | 157 |
| BstX2I | RGATCY | 2 cut(s) | 217, 850 |
| BstYI | RGATCY | 2 cut(s) | 217, 850 |
| BsuRI | GGCC | 3 cut(s) | 30, 80, 862 |
| BtsCI | GGATG | 2 cut(s) | 221, 669 |
| BveI | ACCTGC | 1 cut(s) | 578 |
| Cac8I | GCNNGC | 2 cut(s) | 82, 563 |
| CciI | TCATGA | 1 cut(s) | 712 |
| CseI | GACGC | 1 cut(s) | 267 |
| Csp6I | GTAC | 6 cut(s) | 40, 321, 444, 471, 544, 658 |
| CviAII | CATG | 9 cut(s) | 187, 208, 248, 447, 562, 684, 713, 781, 911 |
| CviQI | GTAC | 6 cut(s) | 40, 321, 444, 471, 544, 658 |
| DdeI | CTNAG | 3 cut(s) | 751, 820, 899 |
| DpnI | GATC | 7 cut(s) | 45, 219, 415, 682, 732, 852, 948 |
| DpnII | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| EaeI | YGGCCR | 2 cut(s) | 78, 860 |
| Eam1104I | CTCTTC | 3 cut(s) | 134, 318, 909 |
| EarI | CTCTTC | 3 cut(s) | 134, 318, 909 |
| Ecl136II | GAGCTC | 1 cut(s) | 139 |
| Eco105I | TACGTA | 1 cut(s) | 543 |
| Eco130I | CCWWGG | 1 cut(s) | 439 |
| Eco147I | AGGCCT | 1 cut(s) | 30 |
| Eco24I | GRGCYC | 1 cut(s) | 141 |
| Eco53kI | GAGCTC | 1 cut(s) | 139 |
| Eco57I | CTGAAG | 1 cut(s) | 187 |
| EcoICRI | GAGCTC | 1 cut(s) | 139 |
| EcoRI | GAATTC | 1 cut(s) | 814 |
| EcoRII | CCWGG | 1 cut(s) | 48 |
| EcoT14I | CCWWGG | 1 cut(s) | 439 |
| EcoT38I | GRGCYC | 1 cut(s) | 141 |
| ErhI | CCWWGG | 1 cut(s) | 439 |
| FaeI | CATG | 9 cut(s) | 190, 211, 251, 450, 565, 687, 716, 784, 914 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 137, 169, 149, 181 |
| FaqI | GGGAC | 2 cut(s) | 400, 781 |
| FatI | CATG | 9 cut(s) | 186, 207, 247, 446, 561, 683, 712, 780, 910 |
| FauI | CCCGC | 1 cut(s) | 672 |
| Fnu4HI | GCNGC | 3 cut(s) | 6, 78, 774 |
| FokI | GGATG | 2 cut(s) | 208, 656 |
| FriOI | GRGCYC | 1 cut(s) | 141 |
| Fsp4HI | GCNGC | 3 cut(s) | 6, 78, 774 |
| FspBI | CTAG | 4 cut(s) | 395, 440, 926, 955 |
| GluI | GCNGC | 3 cut(s) | 6, 78, 774 |
| HaeIII | GGCC | 3 cut(s) | 30, 80, 862 |
| HapII | CCGG | 4 cut(s) | 24, 72, 102, 728 |
| HgaI | GACGC | 1 cut(s) | 267 |
| Hin1I | GRCGYC | 1 cut(s) | 35 |
| Hin1II | CATG | 9 cut(s) | 190, 211, 251, 450, 565, 687, 716, 784, 914 |
| HinfI | GANTC | 1 cut(s) | 940 |
| HpaII | CCGG | 4 cut(s) | 24, 72, 102, 728 |
| HphI | GGTGA | 2 cut(s) | 308, 428 |
| Hpy166II | GTNNAC | 2 cut(s) | 291, 923 |
| Hpy188I | TCNGA | 3 cut(s) | 184, 601, 619 |
| Hpy188III | TCNNGA | 5 cut(s) | 225, 673, 713, 758, 944 |
| Hpy8I | GTNNAC | 2 cut(s) | 291, 923 |
| Hpy99I | CGWCG | 6 cut(s) | 13, 40, 98, 283, 301, 831 |
| HpyAV | CCTTC | 2 cut(s) | 100, 163 |
| HpyCH4III | ACNGT | 3 cut(s) | 320, 325, 406 |
| HpyCH4IV | ACGT | 3 cut(s) | 35, 123, 542 |
| HpyCH4V | TGCA | 3 cut(s) | 521, 575, 776 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 68, 77, 204, 466, 868 |
| HpyF3I | CTNAG | 3 cut(s) | 751, 820, 899 |
| HpySE526I | ACGT | 3 cut(s) | 35, 123, 542 |
| Hsp92I | GRCGYC | 1 cut(s) | 35 |
| Hsp92II | CATG | 9 cut(s) | 190, 211, 251, 450, 565, 687, 716, 784, 914 |
| Kzo9I | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| LmnI | GCTCC | 3 cut(s) | 74, 104, 144 |
| Lsp1109I | GCAGC | 1 cut(s) | 760 |
| LweI | GCATC | 2 cut(s) | 610, 938 |
| MaeI | CTAG | 4 cut(s) | 395, 440, 926, 955 |
| MaeII | ACGT | 3 cut(s) | 35, 123, 542 |
| MaeIII | GTNAC | 4 cut(s) | 239, 400, 434, 493 |
| MalI | GATC | 7 cut(s) | 45, 219, 415, 682, 732, 852, 948 |
| MbiI | CCGCTC | 2 cut(s) | 77, 667 |
| MboI | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| MboII | GAAGA | 8 cut(s) | 121, 157, 193, 259, 305, 362, 888, 926 |
| MflI | RGATCY | 2 cut(s) | 217, 850 |
| MhlI | GDGCHC | 1 cut(s) | 141 |
| MlsI | TGGCCA | 1 cut(s) | 862 |
| MluCI | AATT | 3 cut(s) | 522, 793, 814 |
| MluNI | TGGCCA | 1 cut(s) | 862 |
| MmeI | TCCRAC | 2 cut(s) | 207, 624 |
| MnlI | CCTC | 8 cut(s) | 20, 95, 125, 137, 182, 221, 352, 829 |
| Mox20I | TGGCCA | 1 cut(s) | 862 |
| MscI | TGGCCA | 1 cut(s) | 862 |
| MseI | TTAA | 1 cut(s) | 330 |
| MslI | CAYNNNNRTG | 1 cut(s) | 375 |
| Msp20I | TGGCCA | 1 cut(s) | 862 |
| MspA1I | CMGCKG | 1 cut(s) | 773 |
| MspI | CCGG | 4 cut(s) | 24, 72, 102, 728 |
| MspR9I | CCNGG | 1 cut(s) | 50 |
| Mva1269I | GAATGC | 1 cut(s) | 708 |
| MvaI | CCWGG | 1 cut(s) | 50 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 68, 77, 204, 466, 868 |
| NdeII | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| NlaIII | CATG | 9 cut(s) | 190, 211, 251, 450, 565, 687, 716, 784, 914 |
| NlaIV | GGNNCC | 4 cut(s) | 100, 219, 697, 884 |
| NmuCI | GTSAC | 2 cut(s) | 400, 434 |
| NspI | RCATGY | 2 cut(s) | 190, 565 |
| NspV | TTCGAA | 1 cut(s) | 874 |
| PaeI | GCATGC | 1 cut(s) | 565 |
| PagI | TCATGA | 1 cut(s) | 712 |
| PceI | AGGCCT | 1 cut(s) | 30 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 180 |
| PctI | GAATGC | 1 cut(s) | 708 |
| PfeI | GAWTC | 1 cut(s) | 940 |
| Pfl23II | CGTACG | 1 cut(s) | 39 |
| PkrI | GCNGC | 3 cut(s) | 7, 79, 775 |
| Ple19I | CGATCG | 1 cut(s) | 46 |
| Ppu21I | YACGTR | 1 cut(s) | 543 |
| PshAI | GACNNNNGTC | 1 cut(s) | 62 |
| Psp124BI | GAGCTC | 1 cut(s) | 141 |
| Psp6I | CCWGG | 1 cut(s) | 48 |
| PspGI | CCWGG | 1 cut(s) | 48 |
| PspLI | CGTACG | 1 cut(s) | 39 |
| PspN4I | GGNNCC | 4 cut(s) | 100, 219, 697, 884 |
| PsuI | RGATCY | 2 cut(s) | 217, 850 |
| PvuI | CGATCG | 1 cut(s) | 46 |
| PvuII | CAGCTG | 1 cut(s) | 773 |
| RsaI | GTAC | 6 cut(s) | 41, 322, 445, 472, 545, 659 |
| RsaNI | GTAC | 6 cut(s) | 40, 321, 444, 471, 544, 658 |
| RseI | CAYNNNNRTG | 1 cut(s) | 375 |
| SacI | GAGCTC | 1 cut(s) | 141 |
| SaqAI | TTAA | 1 cut(s) | 330 |
| SatI | GCNGC | 3 cut(s) | 6, 78, 774 |
| Sau3AI | GATC | 7 cut(s) | 43, 217, 413, 680, 730, 850, 946 |
| ScrFI | CCNGG | 1 cut(s) | 50 |
| SduI | GDGCHC | 1 cut(s) | 141 |
| SfaNI | GCATC | 2 cut(s) | 610, 938 |
| SfcI | CTRYAG | 1 cut(s) | 253 |
| SfuI | TTCGAA | 1 cut(s) | 874 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 375 |
| SnaBI | TACGTA | 1 cut(s) | 543 |
| SpeI | ACTAGT | 1 cut(s) | 394 |
| SphI | GCATGC | 1 cut(s) | 565 |
| Sse9I | AATT | 3 cut(s) | 522, 793, 814 |
| SseBI | AGGCCT | 1 cut(s) | 30 |
| SsiI | CCGC | 7 cut(s) | 5, 62, 77, 142, 604, 665, 706 |
| SspI | AATATT | 1 cut(s) | 741 |
| SspMI | CTAG | 4 cut(s) | 395, 440, 926, 955 |
| SstI | GAGCTC | 1 cut(s) | 141 |
| StuI | AGGCCT | 1 cut(s) | 30 |
| StyD4I | CCNGG | 1 cut(s) | 48 |
| StyI | CCWWGG | 1 cut(s) | 439 |
| TaaI | ACNGT | 3 cut(s) | 320, 325, 406 |
| TaiI | ACGT | 3 cut(s) | 38, 126, 545 |
| TaqI | TCGA | 2 cut(s) | 826, 874 |
| TasI | AATT | 3 cut(s) | 522, 793, 814 |
| TauI | GCSGC | 2 cut(s) | 8, 80 |
| TfiI | GAWTC | 1 cut(s) | 940 |
| Tru1I | TTAA | 1 cut(s) | 330 |
| Tru9I | TTAA | 1 cut(s) | 330 |
| TseFI | GTSAC | 2 cut(s) | 400, 434 |
| TseI | GCWGC | 1 cut(s) | 773 |
| Tsp45I | GTSAC | 2 cut(s) | 400, 434 |
| TspDTI | ATGAA | 4 cut(s) | 97, 359, 701, 927 |
| XapI | RAATTY | 1 cut(s) | 814 |
| XceI | RCATGY | 2 cut(s) | 190, 565 |
| XmaJI | CCTAGG | 1 cut(s) | 439 |
| XspI | CTAG | 4 cut(s) | 395, 440, 926, 955 |
| ZraI | GACGTC | 1 cut(s) | 36 |